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The regulatory mechanisms of azoximera bromide on the characteristics of cell's phases experimental microbic inflammation with indicate concentrations of interleukin-1β, interleukin-10, corticosterone are presented. The inflammation of staphylococcus is a chronic process, however azoximera bromide shorten time cell's phases of inflammation, activate functions of inflammatory cells, optimizate synthesis of interleukins and corticosterone, preclude from chronization inflammation.

The characteristics of cell's phases experimental aseptic inflammation with indicate levels of interleukin-1β, interleukin-10, corticosterone are presented. The area of aseptic inflammation, have logical relay of cell's populations, IL-1β, IL-10, corticosterone are regulate cell's processes. And by 20 days inflammatory process complete formation dense connective capsule.

The problem of frequent comorbidity between depression and metabolic syndrome has been known to medicine for a long time. However, there is more and more data on the joint course of these states. The narrative review presents current scientific data on the relationship between metabolic syndrome and depression, as well as the results of studies on the effect of antidepressants on the development of metabolic disorders. The review is based on an analysis of 75 publications published in the bibliographic databases of the RSCI and Medline. Despite numerous research results proving the comorbidity of depression and metabolic syndrome, the question of their causal relationship remains open. The presented materials complement the understanding of the associations of metabolic syndrome and depression, and also allow us to take into account the mechanisms of comorbidity of these conditions when choosing antidepressants.

Background. Research results on the immune response and cytokine profile in children with the new coronavirus infection SARS-CoV-2 are contradictory and insufficient, which necessitates the need to understand the nature of the course of the disease depending on age and severity of the infection. The importance of assessing risks and predicting the consequences of a new coronavirus infection in childhood makes the study of serum concentrations of pro-inflammatory and anti-inflammatory cytokines relevant.The aim. To study the level of pro-inflammatory and anti-inflammatory cytokines in newborns during the acute period of a new coronavirus infection to determine the characteristics of the immune response and identify prognostic criteria for the course of the infectious process.Materials and methods. The article presents the results of a study of the level of pro-inflammatory, anti-inflammatory cytokines and C-reactive protein (CRP) in newborns (n = 44) hospitalized with a confirmed new coronavirus infection of varying severity. The study was conducted during the acute period of COVID-19.Results. Cytokine concentrations in the blood serum of newborns were determined by enzyme immunoassay. In newborns in the acute period of a new coronavirus infection, regardless of the severity, there is a decrease in serum concentrations of interleukin-1β, interleukin-4, interleukin-6, interleukin-8, tumor necrosis factor α, interferon (INF) γ and CRP compared with the control group. The level of INF-α in the blood serum of newborns of both the main and control groups did not have significant differences.Conclusions. The cytokine profile in newborns with the new coronavirus infection SARS-CoV-2 may be due to inhibition of the innate immune system, which requires further research in this direction and increased pediatric attention to this category of children.

Background. Even small amounts of alcohol have an extremely negative effect on the fetus, especially in the early stages of gestation. It is known that ethanol promotes the formation of free radicals, but there is no data on the nature of the oxidative stress development in pregnant women depending on its blood level.The aim. To assess the level lipid peroxidation products and superoxide dismutase activity in the first trimester of pregnancy for women, who consumed alcoholic bevera ges, depending on the phosphatidylethanol (PEth) level in the blood.Materials and methods. The study included 165 women in the first trimester of pregnancy aged 18 to 40 years. To identify the fact and amount of alcohol consumption, the direct biomarker of alcohol 16:0/18:1PEth in blood plasma was performed using high-performance liquid chromatography with tandem mass spectrometry. Depending on the concentration of PEth, groups of women consuming different doses of alcohol were identified: group 1 – PEth concentration < 8 ng/ml (non-drinkers; n = 63); group 2 – PEth concentration from 8 to 45 ng/ml (drinkers of less than 1 dose; n = 66); group 3 – PEth concentration ≥ 45 ng/ml (drinkers of more than 1 dose; n = 36). The content of lipid peroxidation products and superoxide dismutase activity was determined by spectrophotometric methods.Results. It was found that lipid peroxidation intermediates level in the groups of women who consume alcohol, regardless of PEth 16:0/18:1 level in blood plasma, was significantly higher compared with the control (p < 0.05). The superoxide dismutase activity was higher in the group of pregnant women who consumed less than 1 dose of alcohol compared with the control (p < 0.05) and the group who consumed one or more doses of alcohol (p < 0.05).Conclusion. The obtained results indicate the activation of lipid peroxidation processes in the first trimester of pregnancy regardless of the dose of alcohol-containing products consumed.

Objective. Currently, in Russia, as well as all over the world, an increase in morbidity and mortality from pathologies of the cardiovascular system is registered. Employees of petrochemical production are exposed to a large number of additional factors of the production environment, which in turn increases the cardiovascular risk in this cohort of the population. Petrochemistry is also one of the main industries of great economic and social importance for our country, which makes the study of this problem more relevant.Results. According to the results of many studies, the most common cardiovascular pathology in the studied population is arterial hypertension. Many researchers associate the high prevalence of this nosology not only with the direct influence of production factors, but also with the number of years worked at petrochemical enterprises. The main production factors contributing to the development of circulatory system diseases in petrochemicals employees were identified, for example, noise, exposure to chemical harmful substances, increased stress levels and tension during the work process. These factors act on the worker's body constantly and contribute to an increased risk of developing cardiovascular diseases. The main aspects of the pathological influence of production factors on the health of the workers' organism are analyzed. With the help of the obtained data, ways to solve the problem under study were proposed. The presented materials will help to develop measures for the prevention of the development of diseases of the circulatory system in more detail and narrowly, taking into account the influence of not only the main known risk factors, but additional impacts during the labor process.Conclusion. The proposed measures should be aimed at modifying and improving existing preventive and hygienic measures, as well as contribute to improving the health of petrochemical production workers and reducing the incidence of these nosologies, which in turn will prolong the working capacity of employees and reduce economic losses in production.

Currently, there is no common understanding of the bioelement status of obese children and adolescents. In this regard, we have summarized the available data on open access and presented information on the role and content of bioelements involved in the regulation of free radical homeostasis in the genesis of childhood obesity. The literature review revealed modern ideas about obesity as a metabolic pathology. The multifactorial nature of the development of oxidative stress in obesity, including in children and adolescents, is considered, the role of bioelements in the regulation of free radical homeostasis is revealed and it is shown that they can act as cofactors of antioxidant enzymes and/or be initiators of oxidative reactions. The most significant bioelements involved in the regulation of free radical processes are copper, zinc, manganese, selenium and iron. The analysis of the literature shows that children and adolescents with obesity experience changes in the metabolism of the main bioelements involved in the regulation of redox homeostasis. The content of copper, zinc, selenium, manganese and iron may vary depending on the gender and age of the subjects, as well as the type of biomaterial being studied.

Influenza and acute respiratory viral infections (ARVI) impose a substantial damage to the population health in the Russian Federation due to their seasonal circulation and predominantly affect young children. A very few data on the cytokine profile, the nonspecific LPO — AOD system and their relationships with clinical characteristics in such diseases in preschool children are available. The aim of this study was to assess the cytokine profile, LPO — AOD system parameters and their relationship with the clinical and laboratory characteristics of diseases in preschool children with influenza and other ARVI. 86 preschool children (3–6 years old) were examined: with an established diagnosis of influenza (n = 31), non-influenza ARVI (n = 28), apparently healthy children (control group (n = 27). All pediatric samples were analyzed by enzyme-linked immunosorbent assay assessing blood serum concentrations of C-reactive protein and cytokines IL-1β, IL-4, IL-6, IL-8, TNFα, IFNα, IFNγ. Spectrophotometric, fluorometric and enzyme immunoassay methods to assess the state of the “LPO — AOD” system were used. In the group of children with influenza vs other ARVI, a higher incidence of intoxication syndrome was revealed. Cytokine profile in children from both clinical groups compared with control cohort was featured with higher indicators of both pro-inflammatory and anti-inflammatory origin. Children with non-influenza ARVI, had increased magnitude of LPO final products in the nonspecific LPO — AOD system along with lowered concentration of fat-soluble vitamins, general antioxidant activity, GSH level, and SOD activity. In the group with influenza, the level of primary and final lipid peroxidation products was increased, whereas that of for retinol,α-tocopherol, and total antioxidant activity was decreased paralleled with higher GSSG and SOD levels. Numerous correlations were noted in the group of children with ARVI: IL-1β/ketones, IL-6/ketones, IL-8/ketones, TNFα/ketones, IL-4/ketones, IFNg/shortness of breath, IFNα/cough, double bonds/fever, double bonds/AST, SO/intoxication, retinol/fever, GSSG/cough. The influenza group differed in the following relationships: IL-4/ketones, IL-4/fever, IFNα/ketones, CDs/AST. It can be concluded that in preschool children with ARVI and influenza, changes in the cytokine profile are accompanied by increased pro- and anti-inflammatory cytokine levels, increased intensity of lipid peroxidation reactions along with reduced magnitude of antioxidant factors. In the group with ARVI, there was a relationship between the final toxic products of lipid peroxidation — Schiff bases — and the intoxication index, as well as the presence of protective mechanisms in the form of connections between interferons and disease clinical manifestations. The group with influenza was distinguished by the presence of protective relations, which may have a beneficial effect in the context of developing pathological process. The data obtained will help expand the understanding of the pathogenetic mechanisms related to immune reactivity and nonspecific lipid peroxidation reactions in preschool patients and formulate appropriate measures for correction.

Background. In recent years, special attention has been paid to the studying the consortia of probiotic bacteria. In these associations, the properties of individual microorganisms can be enhanced, in particular, their antagonistic activity which is an effective indicator for screening of probiotic potential. The development of probiotics based on such consortia with antibacterial properties is critical in the light of the growing problem of drug resistance in microorganisms.The aim of the work. To study the antagonistic activity of monocultures and consortia of lactobacilli against multidrug-resistant isolates of opportunistic bacteria. Materials and methods. The antagonistic activity of lactobacilli monocultures and their consortia was assessed simultaneously by two methods: the cross streak method and the well diffusion method.Results. All strains of lactobacilli and their consortia, depending on the research method, had varying degrees of antagonistic activity. Five consortia had stronger antagonism to test cultures as compared to monocultures, while in one consortium, the effect of antagonistic activity was reduced compared to monocultures. The results of studying the antagonistic activity of two consortia (Limosilactobacillus fermentum 44/1 and Lacticaseibacillus rhamnosus 12L, Latilactobacillus curvatus LCR-111-1 and Lactiplantibacillus plantarum 8PAZ) contradict data on the biocompatibility of strains in these consortia. Differences in the degree of antagonistic effects of lactobacilli on gram-positive and gram-negative species of opportunistic bacteria were revealed.Conclusion. The study showed that both the biocompatibility of the probiotic strains and the antagonistic activity of the consortium are the important requirements for creating a probiotic consortium with effective probiotic potential. To study the antagonistic properties of lactobacilli, the number of isolates of target gram-positive and gram-negative bacteria and normobiota should be increased. This will allow us to determine effective strategies for using probiotics in conditions of the spread of drug resistance of microorganisms.

The aim. The research was conducted to assess the total antioxidant and glutathione status, superoxide dismutase activity in menopausal women with moderate and asymptomatic COVID-19.Materials and methods. Ninety two women 45 to 69 years old were divided into groups: women without COVID-19, not vaccinated, with no antibodies to SARS-CoV-2 (IgG) – control; women with moderate COVID-19 – main group; women with anti-SARS-CoV-2 IgG in blood but who deny any symptoms of COVID-19 in the last 12 months – asymptomatic COVID-19.Results. A lower glutathione peroxidase (GPx), superoxide dismutase (SOD) activities and a higher glutathione reductase (GR) activity, glutathione S-transferase pi (GSTpi) concentrations were detected in the patients with moderate COVID-19 as compared to control. There were statistically lower oxidized glutathione (GSSG) levels, total antioxidant status (TAS) and higher reduced glutathione (GSH) levels, as well as GSH/GSSG ratio in the group with asymptomatic COVID-19 as compared to control. Significantly a lower GPx, SOD activities and a higher TAS, GR activity, GSTpi concentrations were detected in the patients with symptomatic COVID-19 as compared to group without clinical symptoms. ROC analysis shows the diagnostic significance of TAS (AUC = 0.714; p = 0.048), GSH (AUC = 0.714; p = 0.030), GSSG (AUC = 0.712; p = 0.031), GSH/GSSG (AUC = 0.837; p < 0.001) for group with asymptomatic COVID-19 compared with controls; TAS (AUC = 0.709; p = 0.020), SOD (AUC = 0.760; p < 0.001), GSH/GSSG (AUC = 0.658; p = 0.039), GPx (AUC = 0.774; p < 0.001), GSTpi (AUC = 0.864; p < 0.001) and GR (AUC = 0.871; p < 0.001) for group with moderate COVID-19 compared asympotmatic COVID-19.Conclusions. Antioxidant defense system activity in menopausal women depends on the COVID-19 course.

In recent years, studies of the human microbiome have led to the development of the gut-brain axis theory, which proposes a relationship between the gut bacterial community and its metabolites and chemical compounds, as well as physiological and pathological processes in the brain. Melatonin is considered one of the potential links in this relationship.The review provides brief information about the synthesis, metabolism and effects of melatonin in the body. General patterns of the effect of melatonin on the intestinal microbiota under various conditions, such as oral and intraperitoneal melatonin administration, sleep deprivation, as well as the results of studying the microbial community with measurement of hormone levels are considered.Results are presented for families and individual genera of microorganisms and are obtained from studies in animal models or in the research of diseases. The article also notes some additional parameters which are supposed to be relevant to the interaction of melatonin with gut microbiome. Special attention is paid to butyrate as one of the most studied possible parts in this interaction. Studying the relationship between melatonin and microbiome is of interest for basic science and practical medicine.Literature search was carried out using electronic information resources PubMed and eLibrary.

The literature review examines the problem of obesity in modern society. It has been shown that obesity aggravates concomitant diseases, increases the probability of developing metabolic disorders and related pathologies, increases the risk of complications and mortality. The secretory function of adipose tissue, its participation in the regulation of biological processes is considered in detail. The concept of carbonyl stress and its components is revealed, the role of carbonyl compounds in the body is described, the metabolic pathways leading to the formation of carbonyl reaction products are shown, the participation of free radicals in these metabolic pathways is noted. The mechanisms of pathogenesis associated with the development of carbonyl stress in obesity are discussed; the greatest contribution to the development of car bonyl pathology in obesity is made by two types of processes: lipid peroxidation reactions resulting in the formation of carbonyl products of lipoperoxidation and the processes activated by hyperglycemia (glycolysis, polyol and hexоzamine pathways) leading to the formation of glyoxal, methylglyoxal, and active carbonyl forms of glucose. The question of the contribution of advanced glycation end products (AGEs) and advanced oxidation protein products (AOРР) to the development of carbonyl pathology in obesity remains controversial. It is assumed that AGEs and AOРР levels depend on the severity of obesity and the development of metabolic syndrome.

Introduction. Respiratory diseases occupy a leading place in the structure of pathology of childhood. The proportion of influenza and acute respiratory viral infections among all infectious diseases is 90 %. The literature describes gender differences in the immune response to respiratory infections in children, but there is a gap in the description of the cytokine profile in children with influenza depending on gender and age.The aim. To analyze clinical and laboratory parameters as well as cytokine profile parameters in children with influenza.Materials and methods. A single-stage descriptive study was conducted with the participation of 50 children from 1 to 11 years of age with a diagnosis of influenza who were on inpatient treatment at the Irkutsk Regional Infectious Diseases Clinical Hospital from December 2018 to January 2019. The clinical and laboratory features of the course of influenza in children, the duration of treatment were determined. The concentration of cytokines interleukin (IL) 1β, IL-4, IL-6, IL-8, tumor necrosis factor alpha (TNF-α), interferon alpha and gamma (INF-α, INF-γ) in blood plasma was determined by enzyme-linked immunosorbent assay (ELISA) using diagnostic test systems manufactured by Vector-Best (Novosibirsk, Russian Federation) on the analyzer Multiscan EX (Thermo Electron, Germany).The control group consisted of practically healthy children without signs of acute respiratory viral infection (n = 50; mean age 5.3 ± 2.6 years).Results. When comparing clinical and laboratory data and cytokine profile parameters in children with influenza, no gender differences were found. There was a statistically significant increase in the level of pro-inflammatory cytokines IL-1β, IL-6, IL-8, TNF-α, INF-α, as well as CRP, anti-inflammatory cytokine IL-4 in influenza in all age categories, in contrast to the control group (p < 0.05).Conclusion. Influenza in children of different sexes proceeds classically without a statistical difference in clinical and laboratory parameters and in the level of cytokines.

The review is devoted to the classification, diagnosis and differential diagnosis of transient loss of consciousness. The pathophysiology of transient loss of consciousness is described. It is concluded that the criteria for the differential diagnosis of transient disorders of consciousness need further development and validation. The problem of paroxysmal disorders of consciousness is interdisciplinary in nature and requires the combined efforts of doctors of different specialties to develop a unified algorithm for clinical, laboratory and instrumental diagnostics, determine management tactics and correct patient routing.

Own and literature data showed a change in the free radical oxidation products and indicators of the glutathione system concentration in menopausal women with insomnia. The change in the studied parameters depended on the ethnicity of the patients. The aim of the study was to assess the informative value of these indicators and their contribution to the state of free radical homeostasis in menopausal women of Russian and Buryat ethnic groups with insomnia. Material and methods. The study involved women of the menopausal period of the Russian (n = 136) and Buryat ethnos (n = 94). The women underwent a general clinical examination and a questionnaire. Then they were divided into climacteric phases, control groups and groups with insomnia. Enzyme immunoassay, spectrophotometric and fluorometric methods of analysis were used to determine values of glutathione system and biomolecule oxidation products. The informative value of the studied indicators and their contribution to the state of free-radical homeostasis was assessed with the help of discriminant analysis. Results and discussion. The most informative indicators in the group of Russian women with insomnia in perimenopause were content of advanced glycation end products (AGEs), advanced oxidation protein products (AOPP), products of DNA oxidative modification, oxidized glutathione, glutathione reductase activity; in postmenopause – concentration of AOPP, products of DNA oxidative modification, and glutathione reductase activity. In Buryat perimenopausal women the most informative values were content of AGEs, of reduced and oxidized glutathione and their ratio, and in postmenopause – content of AGEs, of oxidized glutathione and glutathione reductase activity. Mathematical models have been developed that demonstrate the prevalence of the redox balance prooxidant component contribution in Russian ethnic group and the antioxidant component in Buryat ethnic group and allow characterizing patients with insomnia with high accuracy depending on the phase of menopause and ethnicity. Conclusions. In women of the Russian ethnic group with insomnia in the menopausal period, the greatest contribution to the state of free radical homeostasis is made by the products of oxidation of biomolecules, which characterizes the predominance of the processes of disadaptation. In women of the Buryat ethnic group, in response to insomnia in the climacteric period, the components of the glutathione system make the greatest contribution to the state of free radical homeostasis, which characterizes the activation of adaptive capabilities throughout the climacteric period.

Background. Studying the problem of alcohol consumption by pregnant women using modern laboratory research methods has valuable theoretical and practical significance. The aim of the study. To determine the characteristics of the pregnancy course in women consuming alcohol in the prenatal period confirmed by survey and laboratory tests, depending on the phosphatidylethanol levels. Materials and methods. We examined 863 women under observation at the Irkutsk Regional Perinatal Center for the period from 2014 to 2021. To confirm the fact of alcohol consumption in the prenatal period, 545 women were surveyed, 318 women were examined using laboratory analysis. The diagnostic biomarker for alcohol was PEth:16:0/18:1. To assign pregnant women to the control group, a PEth concentration of ≤ 8 ng/ml was taken. If the PEth concentration was > 8 ng/ml, pregnant women were classified as heavy drinkers. Clinical and laboratory indicators of the course of pregnancy and childbirth were carried out in comparative groups. Results. It has been established that every second woman of reproductive age took alcohol before pregnancy. 24.2 % of women did not stop consuming alcohol in the prenatal period. At the same time, the risk of congenital malformations was high, since 20.4 % of women consumed alcohol in the first trimester of pregnancy. Based on the results of the survey, it was revealed that in women who consumed alcohol in the prenatal period, the following pathological conditions are statistically significantly more common: anemia, congenital heart defects in fetuses, prematurity of gestational age, labor anomalies, uterus subinvolution. Based on the results of laboratory confirmation of alcohol consumption, it was established that parity of birth, intrauterine growth retardation, and premature birth were statistically significantly more often in pregnant women who drink. Conclusion. Thus, in order to obtain the most meaningful and high-quality results, it is necessary to conduct larger studies. In addition, maternal blood biomarkers should be used to confirm levels of alcohol consumption throughout all trimesters of pregnancy.

Background. Using probiotic preparations based on consortia of microorganisms not only helps to restore the balance of the intestinal microbiota, but also increases the therapeutic effect of probiotics. Promising sources for obtaining probiotic consortia are milk products that have undergone natural fermentation with the help of spontaneously formed microbial consortia. The aim. To study the structure of five microbial consortia with probiotic properties from naturally fermented milk products and to assess in vitro their antagonistic activity against opportunistic bacteria and a representative of the human normobiota – Escherichia coli. Materials and methods. The structure of bacterial consortia was analyzed by sequencing methods. The antagonistic activity of the consortia was assessed by the disk diffusion method. Results. It has been established that the studied microbial consortiums are represented by Enterococcus spp. and Streptococcus spp. bacteria. In consortiums No. 1, No. 2, and No. 3, Enterococcus bacteria dominated, while in consortiums No. 4 and No. 5, Streptococcus dominated. Antagonistic activity was shown against four isolates of opportunistic bacteria: Klebsiella pneumoniae No. 493, Enterobacter hormaechei No. 372, Staphylococcus aureus No. 4 and Pseudomonas aeruginosa No. 25 IMB, as well as against one representative of the human normobiota – Escherichia coli No. 495. The highest growth delay zone is found in E. coli No. 495 isolate. Three test cultures (K. pneumoniae No. 509, E. coli ATCC25922 and P. aeruginosa No. 3 IMB) exhibited more dense growth around probiotic consortia. Conclusion. The results of the study showed that the effect of probiotic consortia differing in the composition of microorganisms can be neutral and bactericidal. The presence of antagonistic activity in the studied microbial consortia against multiresistant isolates of opportunistic bacteria is a prospect for creating probiotics with antibacterial properties.

The aim. To assess the thyroid status and its relationship with tumor necrosis factor alpha (TNF-alpha) in post-reproductive women in the acute phase of the COVID-19 of moderate course and 12 months after the disease.Materials and methods. 85 women aged 45 to 69 years were divided into groups: women without COVID-19, not vaccinated, with no antibodies to COVID-19 (IgG) – control group (n = 15); women in the acute phase of COVID-19 of a moderate course, accompanied by pneumonia – main group (n = 57); patients from the main group who agreed to be examined 12 months after having COVID-19 (n = 14); women with IgG in blood who deny any symptoms of COVID-19 in the last 12 months – asymptomatic COVID-19 (n = 13). Using hormone replacement therapy and the presence of thyroid disease in history were the exclusion criteria from the study.Results. 75.4 % of patients with COVID-19 had euthyroidism, 12.3 % had subclinical hyperthyroidism. An increase of free thyroxine (free T4) level in women with COVID-19 as compared to the control group (p = 0.004) and the group with asymptomatic COVID-19 (p = 0.054) was found. There was no statistically significant difference in the level of thyroid stimulating hormone between the groups. The level of C-reactive protein in women with COVID-19 was naturally higher as compared to the control group (p = 0.009) and the group of asymptomatic patients (p = 0.001). A lower TNF-alpha level was found in the group of patients without clinical signs of COVID-19 as compared to the control group (p = 0.007) and the group with COVID-19 (p = 0.00007). The analysis of correlation relationships revealed a positive correlation between of free T4 and TNF-alpha levels in women with COVID-19 (r = 0.38, p = 0.004).Conclusions. The moderate course of COVID-19 in the post-reproductive women is associated with an increase of free T4 level, which positively correlates with TNF-alpha level. Twelve months after COVID-19, thyroid status in women remains at the level of the acute phase of the disease.

The gut microbiota is the largest human microbial community, comprising more than 1000 species of various microorganisms. It has been established that these are the gut microorganisms that play a decisive role in maintaining health, participating in the metabolism of proteins, carbohydrates, lipids, bile acids and the synthesis of vitamins. It has been shown that changes in the balance in the gut microbiocenosis lead not only to pathologies of the gastrointestinal tract, but also increase the risk of developing diseases of the cardiovascular and endocrine systems, and cause disturbances in the psycho-emotional state. To restore, correct and maintain the gut microbiota, probiotic preparations are recommended. In connection with the obvious relevance of the use of probiotics, this review aimed to describe the history of the emergence of the first probiotic products, current trends in the development of the concept of functional foods enriched with probiotics, as well as the prospects for developing probiotic preparations in the Russian Federation and abroad. This article presents an analysis of the market for probiotic preparations and dietary supplements, as well as functional food products that are produced in the Siberian Federal District. For an objective assessment of the development of the market for probiotic products, a generalization of analytical data from marketing services that monitor the movement of medicines in pharmacy chains and analyze consumer reviews was carried out.

The aim of the study was to assess the functional state of glutathione system in menopausal women of the Buryat ethnic group with insomnia. Methods. The study involved 94 women aged 45 to 60 years who were divided into perimenopause (n = 44) and postmenopause (n = 50) groups. In each period, a control group and an insomniac group were identified. The reduced (GSH) and oxidized (GSSG) glutathione levels in erythrocyte lysate, the glutathione-S-transferase π concentration and glutathione reductase activity in blood serum were determined. Results. An increase of the GSH level (p < 0.05) and GSH/GSSG ratio (p < 0.05) both in perimenopausal and postmenopausal women with insomnia were found as compared to controls. There were no differences in the glutathione-depends enzymes parameters. Comparative analysis of the main groups showed an increase GSH/GSSG ratio (p < 0.05) in the postmenopausal period compared with perimenopause. Conclusion. The study results demonstrate the glutathione link activation in Buryat menopausal women with insomnia. In this regard, the appointment of glutathione preparations for the correction of free radical homeostasis in these groups of women cannot be recommended.

The aim. To assess the general condition, laboratory parameters, quality of life of women of post-reproductive age after asymptomatic COVID-19 and 12 months after moderate COVID-19. Material and methods. We examined 47 women (45–69 years old), divided into 3 groups: those who did not have COVID-19, not vaccinated (group 1 – control; n = 15); women 12 months after moderate COVID-19, accompanied by pneumonia (group 2; n = 19); women with IgG in their blood who deny any symptoms of COVID-19 (group 3; n = 13). Results and discussion. An assessment of the general condition of women in post-reproductive age was carried out. In women 12 months after COVID-19 we found a lower level of total bilirubin compared to the group with asymptomatic COVID-19 (p = 0.004). An increase in thrombin time was determined in the groups of patients with symptomatic (p = 0.014) and asymptomatic (p = 0.025) COVID-19 course compared with the control group. Patients with asymptomatic COVID-19 course had a higher level of high-density lipoprotein cholesterol compared with the control group (p = 0.016) and the group 2 (p = 0.006). Compared with the control group, the group 2 had lower scores for general health (p = 0.006), vital activity (p = 0.013), general physical well-being (p = 0.039), physical functioning (p = 0.046); and the group 3 had higher scores in role functioning due to physical condition (p = 0.049). When comparing groups 2 and 3, lower scores in the group of women who recovered from the moderate COVID-19 were found in physical functioning (p = 0.002), pain intensity (p = 0.034), role functioning due to physical condition (p = 0.049), general health (p = 0.003), vital activity (p = 0.018), general physical well-being (p = 0.001). Conclusion. 12 months after moderate COVID-19, there is a pronounced deterioration in physical and emotional health; and in asymptomatic patients, better protective functions of an organism can be assumed.

The results of a study of the situation of light designation in akathists to Russian saints is presented in the article. The relevance of studying the phenomenon of light on this material is due, firstly, to the most important text-forming role of light designs in this hymnographic genre, and secondly, to the need for an in-depth study of the role of the verb in the organization of the Slavic sentence. The situation of light designation became the direct object of analysis, and the participants in this situation became the subject. The focus of research attention is on the relevant valence (semantic and syntactic) characteristics of the verb as the structural center of the sentence. The main research methods were: functional-semantic, contextual analysis of lexical units, statistical. As a result of the study, a linguistic corpus of light designations (3,157 units) was identified, their part-of-speech affiliation was characterized, a chronological distribution was demonstrated, and the dominant root-forming elements (svet-, siya-, zar-, blist-, svesh-) were established. Particular attention is paid to the grammatical mechanisms that provide light designation, as well as to its direct participants. The emphasis is on the main participant — the light. The description of the register of possible semantic roles of participants in the situation of light designation (Subject, Object and their subtypes) is focused on the division traditional in the Orthodox metaphysics of light: “uncreated light”, “smart light” and “sensual light”.

An increase in peroxidation activity is considered as a nonspecific process characteristic of the pathogenesis of various diseases accompanied by antioxidant deficiency. As bioregulators that can increase defense, antioxidants are important links in a multi-stage system of regulation and coordination of various body functions. The structure and function of enzymes involved in the regulation of oxidative stress can be significantly affected by genetic polymorphism. To date, the role of genes encoding the activity of enzymes of the antioxidant system in the pathogenesis of many diseases remains unexplored, which is of great interest to researchers from various fields. The article presents a review and analysis of data from modern scientific literature on the role of antioxidant defense components in the regulation of metabolic processes, their genetic determinant, and summarizes data on modern methods for the determination of some antioxidants. When writing the review, the database of the scientific electronic library eLibrary was used, the keywords are oxidative stress, free radical oxidation, antioxidant protection, antioxidants, antioxidant enzymes, enzyme genes, antioxidant response element, research methods; filters – publication years 2012–2022, publications with full text, publications available for viewing; English-language database of medical and biological publications, created by the National Center for Biotechnology Information (NCBI), keywords – lipid peroxidation, antioxidant protection, antioxidants, antioxidant enzymes, enzyme genes, oxidative stress, metabolism, methods.

BACKGROUND: Diabetic nephropathy is a specific kidney damage that affects up to 40% of type 1 diabetes mellitus patients. There is still insufficient knowledge about oxidative stress at the different levels of albuminuria.AIM: To assess the indicators of oxidative damage to lipids, DNA and antioxidant defense in men with type 1 diabetes mellitus and albuminuria different levels.MATERIALS AND METHODS: The study was conducted during 2018–2019. The main group included 56 men of reproductive age with type 1 diabetes mellitus (T1DM) divided into 2 groups: 24 patients with albuminuria level A1 (group A1) and 32 patients with albuminuria level A2 (group A2). The control group consisted of 28 healthy men. The oxidative stress indicators content, as well as the activity of antioxidant defense system various links was evaluated. Spectrophotometric, fluorimetric and enzyme immunoassay methods were used.RESULTS: In patients of the A1 group there were higher values of the median of primary — conjugated dienes, secondary — ketodienes and conjugated trienes, final — Schiff bases products and 8-hydroxy-2’-deoxyguanosine in comparison with the control. Similar changes in patients of the A2 group were found. Intergroup differences related to increased median values of TBARs and 8-hydroxy-2’-deoxyguanosine in patients of the A2 group compared with A1 group. The activity of the antioxidant defense system in A1 group relative to the control by increased values of the glutathione peroxidase, reduced glutathione, oxidized glutathione median, and retinol was characterized. A2 group had higher values of glutathione peroxidase, reduced glutathione and oxidized glutathione medians in comparison with controls. Correlation analysis in A1 group showed the relationships between the duration of the disease and the products of lipid peroxidation, glycated hemoglobin with 8-hydroxy-2’-deoxyguanosine. In A2 group, there was a relationship between the albumin/creatinine ratio and the duration of the disease, glomerular filtration rate and creatinine level.CONCLUSION: In men with T1DM, regardless of the albuminuria level, there are higher values of the oxidative damage lipids and DNA parameters, as well as the presence of close relationships between these parameters and the duration of the disease, which can be used to develop potential strategies for the prevention and early therapy of diabetic nephropathy.

Background. The COVID-19 pandemic has raised the importance of this problem to the first stage and has affected healthcare system around the world. Despite the more favorable COVID-19 course, the child population should be at focus of special attention, due to the active participation in its distribution. The course of COVID-19 includes a cascade of pathological processes accompanied by the generation of reactive oxygen species, which can have extremely negative consequences for the developing organism. The research of these processes in children is vital and will improve the effectiveness of preventive and therapeutic measures. The aim: to analyze changes in enzymatic and non-enzymatic links in the antioxidant defense in children and adolescents with diagnosed COVID-19 infection.Materials and methods. 17 children and adolescents (average age – 12.35 ± 4.01 years) were examined, including 8 boys (47 %) and 9 girls (53 %) with COVID-19 infection. The control group of children and adolescents (practically healthy) according to the «copy-pair» principle was selected. We used spectrophotometric methods.Results. In the group of children and adolescents with diagnosed COVID-19 infection, there were lower levels of total antioxidant activity (p < 0.0001), superoxide dismutase activity (p < 0.0001), content of reduced glutathione (p = 0.048) and retinol (p = 0.015), increase in glutathione reductase activity (p = 0.015) relative to the control.Conclusion. The obtained data indicate the insufficiency of antioxidant system components number in children and adolescents with diagnosed COVID-19 infection and indicate the advisability of antioxidant therapy using to stabilize these indicators.

Patients with substance use disorders represent a vulnerable population who are at risk of getting coronavirus due to several factors related to their clinical, psychological, and psycho-social characteristics. The goal of this study was to examine the features of a medical condition in patients with alcohol use disorder who are admitted to the addiction hospital and to describe the specific issues of the addiction care RF region during a pandemic.Material and methods. The online survey has been distributed to 55 addiction psychiatrists from the third-level medical organizations of 4th federal districts (including Northwestern, South, Volga, and North Caucasian) from June 09 to June 20, 2020. The survey consists of 17 items (13 questions were focused on the features of progress and clinical signs of alcohol dependence in hospitalized, in-patient individuals during April-May, 2020; 4 questions were focused on the organizational issues of addiction care in the RF regions during the COVID-19 pandemic).Results. Based on the expert`s assessment, the number of admissions at inpatient and outpatient clinical settings was reduced during the pandemic. In addition, the intensity of alcohol withdrawal among patients who were hospitalized during April-May, 2020 were significantly more severe compared to patients who were hospitalized during February-March, 2020 (Pearson’s chi-squared test). Moreover, alcohol withdrawal was more severe and was associated with alcohol withdrawal delirium (4,7%) or seizures (4,7%) in 9,4% of cases among patients with COVID positive status, which exceeds the data of epidemiological studies. The intensity of alcohol withdrawal delirium among patients with COVID positive status was more severe compared to those who had COVID negative status. During April-June, 2020, the few addiction clinics were temporally closed due to quarantine but a majority of clinics were not (n = 14 (25,5%) vs. n = 41 (74,5%)).Conclusion. During the COVID-19 pandemic, the state of patients hospitalized for treatment of alcohol dependence may be considered more severe, compared to the period February-April 2020.

The purpose of the study was to summarize data from foreign studies about the use of virtual reality (VR) technology in the treatment of patients with various mental disorders.Materials and methods: we selected and analyzed articles from MEDLINE / PubMed databases during the period from 2000 till 2020, as well as relevant references in the bibliography of the analyzed articles. 45 articles were included in further analysis from 575 English-language articles.Results: The use of VR technologies in the treatment of mental disorders appears to be one of the promising directions in psychiatry. The successfulness of these methods in patients with eating disorders, anxiety-phobic disorders, and obsessive-compulsive disorder is supported to be evidentiary information. There is evidence of the effectiveness of such methods in patients with schizophrenia, particularly, one of the most significant results is an increase in social functioning. The absence of side effects data makes it possible to consider t VR therapy as a safe method. It is needed to make further study the areas of application of VR therapy and conduct research to identify possible side effects of this method.Conclusion: The obtained results are important for drawing attention to the prospect of using VR technologies in Russian psychiatry and demonstrate the need for further study of this method.

Background. Insomnia occurs in more than half of menopausal women. These disorders can contribute to a change in the prooxidant-antioxidant balance, causing the damage to structural cellular elements. Currently, there is a lack of research on this issue.Aim. To carry out a comparative analysis of the level of advanced oxidation protein products in in periand postmenopausal women with insomnia.Materials and methods. The study included peri(n = 30) and postmenopausal (n = 60) women, who were divided into 2 groups (control and main groups) in each menopausal phase after being questioned using special sleep questionnaires: Insomnia Severity Index; Epworth Sleepiness Scale; Munich Chronotype Questionnaire. The advanced oxidation protein products (AOPP) levels was determined by immunoenzymatic assay using ImmunDiagnostik (German) kits on a BioTek EL×808 (USA) analyzer. Statistical analysis was performed using Mann – Whitney test.Results. Comparative analysis of the AOPP levels in control groups, depending on the menopausal periods, showed an increase in their levels in the postmenopausal period as compared to perimenopause (p < 0.05). When comparing the AOPP levels between the control and the main group in different menopausal periods, statistically significant differences were revealed only in the perimenopausal period towards a higher content in women with insomnia (p < 0.05). The presence of insomnia in postmenopausal women is accompanied by a higher AOPP levels as compared to the perimenopausal women (p < 0.05).Conclusion. The obtained results indicate the association between insomnia and oxidative proteins modification only in the perimenopausal period.

This review examines the variability of the glutathione system in sleep pathologies. Modern sleep theory assumes a restorative sleep function, including active utilization of oxidants and protection from excessive oxidation. In this review article, we conducted an analysis of domestic and foreign literature and summarized data relating aspects of the functioning of the glutathione system in somnological disorders. Various authors have established systemic oxidative stress in insomnia due to reduced activity of glutathione peroxidase. Also, oxidative stress in insomnia is observed in certain areas of the brain due to a decrease in glutathione levels in them. Studies of the functioning of the glutathione system genes in insomnia, whose polymorphisms may include alleles that inhibit oxidation, are arousing interest. An alternative pattern of changes is observed in obstructive sleep apnea syndrome. The apnea stage affects the indicators of the glutathione system. The values of the glutathione system indicators increase with mild to moderate apnea. This is an adaptive response mechanism. Also, the development of oxidative stress in apnea, which leads to disorders in the glutathione system, is cyclical. As a result, people with apnea do not have sufficient replenishment of the components of the glutathione system during sleep. The existing modification does not allow to fully respond to the intensification of peroxide processes and to restrain the activation of excessive oxidation.

The question of qualifying the meaning of allied means, which in traditional grammar are defined as syntactic markers of “immediate” or “fast” succession, and in the part of corpus studies are considered as connectors of “immediate succession”, is studied. Complex sentences with a conjunction “yedva” [hardly] is the object of the study. The types of taxis relations that are expressed in such sentences are analyzed. The research has shown that the grammatical semantics of sentences with the conjunction of “immediate succession” is determined by a whole complex of categorical features, the most important of which is the linguistic representation of time in events. This feature is an indicator of the category of the time order. It was found that the sentences under consideration signal “disturbance of the natural course of events”, and the marker of such a “failure” is a conjunction “yedva” [hardly]. The authors believe that its semantic function, is complicated by the meta-meaning “exaggeration”, which indicates a special communicative attitude of the speaker. The relevance of the research lies in the consistent functional and semantic interpretation of grammatical phenomena. Such interpretation takes into account the established grammatical tradition of considering syntactic units. In the context of modern linguistic research, the scientific continuity of research paradigms is extremely important.

The article presents the results of a survey of specialists from the Orenburg region who provide assistance to patients with mental and behavioral disorders, regarding the experience of using telemedicine technologies. The purpose of the study was to assess the level of awareness of specialists about new technologies, the relevance of their application in the field of psychiatry, as well as to identify problems that arise in the process of implementing telemedicine in the practice of a doctor. The data obtained indicate a high interest in new technologies among doctors who previously participated in remote consultations. At the same time, among specialists who did not have experience with the telemedicine system, a low level of awareness about its capabilities and distrust of new technologies was revealed. Using the survey, it was possible to identify the main problems of telemedicine faced by specialists of the Orenburg region.

The purpose of the research is to study the efficiency of complex medicinal product for veterinary use "RolfClub 3D shampoo” for treatment of dogs’ and cats’ ectoparasitosis.Materials and methods. Cats and dogs affected by fleas Ctenocephalides felis and C. canis, lice Felicola subrostratus and Trichodectes canis, sucking lice Linognathus setosus were selected for the experiment. Presence of specific disease symptoms (pruritus, rumpled and flat coat, scratchings, and places of hair removal) was noted at the time of visual examination of animals’ dermatic hair-coat covering. All infected animals were divided into experimental and control groups. Complex insectoacaricide drug "RolfClub 3D shampoo” ("SPC "Ecoprom") in the form of solution was administered according to the instruction. Before dressing animals’ dermatic hair-coat covering was richly washed by warm water, then the drug was applied at a rate of 1 ml per 1 kg of body weight spreading throughout surface of dermatic hair-coat covering and slightly rubbing till foaming avoiding contact with eyes, ears, and avoiding licking off drug by animals. Shampoo was carefully washed out with warm water in 5–7 minutes. Zoohygienical shampoo was used for the animals from control group. In 24 hours, 2, 5, 10 days shampoo efficiency was evaluated on the basis of calculation of amount of ectoparasites before and after single dressing by the drug "RolfClub 3D shampoo”.Results and discussion. It has been established that the drug "RolfClub 3D shampoo” has significant insecticide effect. Single animals’ dressing by the drug "RolfClub 3D shampoo” provided death of fleas, sucking lice, lice. In 24 hours, 2, 5, 10 days after beginning of experiment all treated animals were free of ectoparasites (fleas, sucking lice, lice). Therapeutic drug efficiency was 100% in the case of all ectoparasites diseases (ctenocephalosis, linognatozis, trichodectozis). Side effects and events were not identified in the case of drug administration.

The range of adverse environmental factors affecting the health of psychiatrists is quite wide. It includes biological and chemical substances, emotional exhaustion and professional burnout, permanent exposure to stress factors of an aggressive environment, forced working posture, tension of analyzer systems and many others. In addition, psychiatrists work under conditions of high emotional stress, which leads to rapid depletion of the nervous system, the development of a syndrome of professional burnout.