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Abstract September–November (e.g., autumn) 2023 produced the fewest western North Pacific (WNP) tropical cyclones (TCs) since 1951, likely as a joint response to El Niño and a warm phase of the North Pacific Mode (NPM). Decreases in both TC genesis frequency and two genesis potential indices (GPIs) over the western WNP were likely the result of El Niño-induced and warm NPM-induced negative low-level relative vorticity anomalies. Over the eastern WNP, TC genesis and GPI reductions were also associated with vorticity decreases over the eastern WNP, where the TC-suppressing effect of the warm NPM surpassed the TC-favoring effect of El Niño. The changes in vorticity were further linked to anomalous anticyclones centered over the South China Sea and the midlatitude central North Pacific. A linear combination of the responses to El Niño and a warm NPM can explain the changes in TC genesis and low-level circulation over most of the WNP in 2023, except east of 160°E where other climate modes may have played more of a role.

Abstract Hydrate formation in foam drainage gas recovery wells and the shut in accidents caused by plugging have become an important problem that restricts the safe production of natural gas. The blockage and accumulation of hydrates is a gradual problem. This research goes beyond predicting the formation of hydrates and delves deeper into examining the rate of hydrate formation and the degree of pipeline blockage at different wellbore locations. First, the temperature model, pressure model, multiphase flow model, and hydrate plugging model of hydrate formation process are established from the equations of mass conservation, energy conservation, and momentum conservation. Second, an iterative approach is employed to solve the model, with a maximum error of 6.86% in model validation. Finally, sensitivity analysis shows that wellhead temperature, wellhead pressure, and foam viscosity have different effects on hydrate formation, maximum plugging position, and plugging degree. At the same time, combined with the actual drainage and gas production process, and the characteristics of hydrate blockage, proposed hydrate prevention measures can be taken to achieve safe production of natural gas. The research results indicate that a decrease in temperature signifies an increase in undercooling, resulting in an accelerated rate of hydrate formation and an elevated risk of hydrate blockage. The decrease in wellhead pressure leads to a decrease in the rate of hydrate formation and an increase in production, which is beneficial for the hydrate prevention. However, larger pressure differences and gas production rates will put higher requirements on equipment such as well control devices. An increase in foam viscosity will lead to increased pressure, foam compression, reduced drainage capacity, and intensified hydrate generation. Therefore, foam viscosity should be kept as small as possible to keep the foam stable.

Natural gas hydrates represents a huge source of energy. At the same time substantial leakages of natural gas from hydrates contributes significantly to climate changes. One of the most important reasons for these natural gas fluxes is leakage of seawater in to the hydrates from seafloor, through fracture systems. Hydrate dissociates if surrounding seawater is less than hydrate stability limit. Another interesting aspect of natural gas hydrates is the potential for safe CO2 storage. These different aspects of hydrates in natural sediments put demands on thermodynamic models. In addition to accurate description of pressure temperature hydrate stability there also a need to describe hydrate dissociation in concentration gradients towards surrounding water or surrounding gas as two examples. In this work we present new experimental data and an extensive thermodynamic model for hydrate. In contrast to conventional thermodynamic models for hydrate the model is consistent since all thermodynamic properties are derived from the Gibbs free energy. In this work we examine mixtures of CH4, C2H6, N2, CO2 from the China Sea and some synthetic mixtures, using this model. Maximum CO2 content in these mixtures are 60 mol% and the rest is dominated by CH4. Agreement between experimental data and model calculations are generally good and average deviations are below 5.5% for all the systems and conditions examined. Another aspect of the model is the ability for incorporation of effects of mineral surfaces. Specifically it is illustrated that adsorption of water on rust dominates liquid water drop out from gas as compared to water dew-point. Production of natural gas with such high CO2 content requires a strategy for CO2 separation and storage. It is proposed that the CH4 is separated from the C2H6, CO2 and N2 and cracked to H2 and CO2 using steam. Thermodynamic analysis indicates a significant potential for safe CO2 storage in natural gas hydrate and H2 as the only export product.

Most of the implemented marine gas hydrate test exploitation in the world adopt the depressurization method to break down the hydrate in the reservoir into natural gas and then extract it, but because the gas production results are still a certain distance away from the commercial exploitation, and it mainly stays in the stage of theoretical research and trial exploitation. Based on two trial productions in the Shenhu area of the South China Sea, this study established a model for hydrate exploitation and investigated the impact of different well types on the recovery rates of hydrates and free gas in different development layers during depressurization. For the Class 1 hydrate reservoirs, horizontal wells are the optimal solution to extract hydrate and free gas simultaneously when exploiting the hydrate three-phase layer. Meanwhile, the effect of different well spacing in vertical wells on the recovery rate of hydrate and free gas was studied. It is found that the best recovery efficiency is achieved when the spacing between two wells is 80 m. The lower the bottom flow pressure of the well, the higher the production capacity, but its influence is limited.

Mechanical forces affect periodontal health through multiple mechanisms. Normally, mechanical forces can boost soft and hard tissue metabolism. However, excessive forces may damage the periodontium or result in irreversible inflammation, whereas absence of occlusion forces also leads to tissue atrophy and bone resorption. We systemically searched the PubMed and Web of Science databases and found certain mechanisms of mechanical forces on immune defence, extracellular matrix (ECM) metabolism, specific proteins, bone metabolism, characteristic periodontal ligament stem cells (PDLSCs) and non-coding RNAs (ncRNAs) as these factors contribute to periodontal homeostasis. The immune defence functions change under forces; genes, signalling pathways and proteinases are altered under forces to regulate ECM metabolism; several specific proteins are separately discussed due to their important functions in mechanotransduction and tissue metabolism. Functions of osteocytes, osteoblasts, and osteoclasts are activated to maintain bone homeostasis. Additionally, ncRNAs have the potential to influence gene expression and thereby, modify tissue metabolism. This review summarizes all these mechanisms of mechanical forces on periodontal homeostasis. Identifying the underlying causes, this review provides a new perspective of the mechanisms of force on periodontal health and guides for some new research directions of periodontal homeostasis.

Abstract This study investigates the influence of the Northeast China cold vortex (NCCV) on the northward‐moving typhoons (NTCs) over the western North Pacific (WNP). There is a significant inverse relationship between the NCCV during June–September and simultaneous NTCs during 1981–2021. Fewer (more) NTCs are observed during NCCV active (inactive) year a combination of less (more) NTC genesis, particularly over the central Pacific region of 10°–30° N and 130°–150° E, and fewer (more) NTCs moving northwestward and making landfall in coastal regions of the Yellow Sea and Bohai Sea. These regions are characterized by significantly decreased low‐level vorticity and mid‐level humidity, which impedes NTC genesis and notably enhances the deep‐layer subtropical straight westerly steering flow, thus blocking the northward movement of NTCs. These remarkable environmental changes during different NCCV years are clearly linked with the changes of an anomalous anticyclone in the subtropics (20°–30° N, 120°–160° E). In short, more (less) NCCV activity strengthens (weakens) the anomalous anticyclone, resulting in fewer (more) NTCs.

Taking a slope as a unit, the regional vulnerability assessment based on potential disaster intensity is one of the important problems to be solved urgently. In this paper, the city of Xiangxiang in Hunan is selected as the research area. On the basis of susceptibility regionalization with the weighted information value method, the elevation, slope height, slope, slope direction and monthly average rainfall of the highest prone points of slope units are extract one by one as the characteristic parameters, which are put into the BP neural network, PSO-BP neural network, random forest and support vector machine model, respectively. A landslide volume prediction model based on BP neural network algorithm optimized by PSO is constructed through training and precision test comparison. A comprehensive vulnerability evaluation model is established with disaster volume as disaster intensity index and building density, population density and property density as vulnerability indexes. Regional vulnerability evaluation based on potential disaster intensity is carried out for the study area. The divisions of high-vulnerable areas (1.5% of the total area), medium-vulnerable areas (28.5% of the total area) and low-vulnerable areas (70% of the total area) are completed, which realize the organic combination of the disaster intensity of the disaster-causing body and the vulnerability of the disaster-bearing body in the process of regional vulnerability evaluation, and enhance the objectivity and scientific nature of the evaluation.

This paper addresses the issue of uncertainty in the grading of geological hazard susceptibility evaluation factors and introduces the adaptive expansion factor fuzzy coverage grading method (AIFFC) to optimize the grading of geological hazard susceptibility evaluation factors. Taking Xiangxiang City, Hunan Province as the research area, nine evaluation factors, including slope, slope direction, elevation and average annual rainfall, normalized difference vegetation index for land use, roads, faults, lithology, were extracted. The AIFFC method and the natural breakpoint method were used to grade continuous factors. These graded factors were then incorporated into a weighted information model and random forest model to obtain a susceptibility zoning map for the study area. The superiority of the AIFFC classification method was tested through the comparison of single-factor grading results, disaster product ratio analysis,and ROC curve comparison of susceptibility zoning results. Based on AIFFC, the hazard accumulation ratio of the random forest model and the weighted information entropy model in the high susceptibility areas increased by 56.3% and 74.6%, respectively, while in the low susceptibility areas, it decreased by 48% and 58.1%, respectively. The AUC values increased by 7.6% and 2.7%, respectively. The AIFFC classification method is used to optimize the evaluation factor classification of geological disaster susceptibility, which significantly improves the rationality of the evaluation of geological disaster susceptibility.

Plastic waste has caused a global environmental crisis. Biocatalytic depolymerization mediated by enzymes has emerged as an efficient and sustainable alternative for plastic treatment and recycling. However, it is challenging and time-consuming to discover novel plastic-degrading enzymes using conventional cultivation-based or omics methods. There is a growing interest in developing effective computational methods to identify new enzymes with desirable plastic degradation functionalities by exploring the ever-increasing databases of protein sequences. In this study, we designed an innovative machine learning-based framework, named PEZy-Miner, to mine for enzymes with high potential in degrading plastics of interest. Two datasets integrating information from experimentally verified enzymes and homologs with unknown plastic-degrading activity were created respectively, covering eleven types of plastic substrates. Protein language models and binary classification models were developed to predict enzymatic degradation of plastics along with confidence and uncertainty estimation. PEZy-Miner exhibited high prediction accuracy and stability when validated on experimentally verified enzymes. Furthermore, by masking the experimentally verified enzymes and blending them into homolog dataset, PEZy-Miner effectively concentrated the experimentally verified entries by 14∼30 times while shortlisting promising plastic-degrading enzyme candidates. We applied PEZy-Miner to 0.1 million putative sequences, out of which 27 new sequences were identified with high confidence. This study provided a new computational tool for mining and recommending promising new plastic-degrading enzymes.

Purpose: To aim of this study is to assess the impact of an internet-enabled nursing model, led by specialized nurses within a framework of multidisciplinary collaboration, on both, patients diagnosed with hypertension, and their respective caregivers. Methods: A total of 158 patients with hypertension, along with their corresponding caregivers, were meticulously selected and paired. By using a random number table method, participants were allocated into either a control group or an observation group. The control group received conventional health education, blood pressure monitoring, and routine telephone follow-ups administered by designated nurses. Conversely, patients in the observation group underwent treatment within an internet-enabled nursing model, led by specialist nurses within a multidisciplinary collaborative framework. Parameters including systolic and diastolic blood pressure readings of the patients, as well as their scores in compliance with the hypertension treatment, quality of life, and caregiving proficiency of family members, which were meticulously documented prior to intervention (T0), as well as at 3- and 6-month intervals post-intervention (T1 and T2). Results: Statistically significant differences were observed in both systolic and diastolic blood pressure levels among patients, as well as in their scores reflecting compliance with hypertension treatment, quality of life, and caregiving proficiency of family members, when comparing pre- and post-intervention periods within each group, across groups, and within the interaction effect (p

Introduction: Chronic hepatitis B (CHB) is a worldwide infectious disease caused by hepatitis B virus (HBV). Optimizing antiviral treatment strategies could improve the functional cure (FC) rate of patients with CHB. This study aims to systematically review the FC rate of the de novo combination of nucleos(t)ide analogs (NAs) and pegylated interferon α (PEG-IFNα) versus that of PEG-IFNα monotherapy for CHB.Methods: Databases were searched until 31 December 2023. Selected studies included randomized controlled trials on the de novo combination of NAs and PEG-IFNα versus PEG-IFNα monotherapy for 48 weeks in patients with CHB to achieve FC, which was defined as hepatitis B surface antigen (HBsAg) loss and/or HBsAg seroconversion. Meta-analysis was conducted in accordance with the efficacy at the end of treatment and different time points during follow-up.Results: A total of 10 studies, encompassing 2,339 patients in total, were included. Subgroup analysis was conducted in accordance with whether first-line NAs were used. It found no statistically significant difference between HBsAg loss and HBsAg seroconversion at the end of treatment. Serum HBV DNA

As an increasingly well-known derivative of coumarin, daphnetin (7,8-dithydroxycoumarin) has demonstrated various pharmacological activities, including anti-inflammation, anti-cancer, anti-autoimmune diseases, antibacterial, organ protection, and neuroprotection properties. Various studies have been conducted to explore the action mechanisms and synthetic methods of daphnetin, given its therapeutic potential in clinical. Despite these initial insights, the precise mechanisms underlying the pharmacological activities of daphnetin remain largely unknown. In order to address this knowledge gap, we explore the molecular effects from the perspectives of signaling pathways, NOD-like receptor protein 3 (NLRP3) inflammasome and inflammatory factors; and try to find out how these mechanisms can be utilized to inform new combined therapeutic strategies.

Abstract Natural gas hydrate saturation (NGHS) in reservoirs is one of the critical parameters for evaluating natural gas hydrate resource reserves. Current widely-accepted evaluation methods developed for evaluating conventional natural gas saturation in reservoirs, to some extents, are not sufficient enough to obtain accurate predicted results. In light of the equivalent medium theory, the natural gas hydrate is regarded as the fluid (Mode A) when NGHS is relatively low, while it is regarded as the rock matrix (Mode B) when NGHS is high. Two mathematical model are then developed for evaluating NGHS at Mode A and B. Experimental verification shows that R 2 of the predicted results based upon the proposed model is 0.968, and the average absolute relative error percentage is 8.90%. The error of the predicted results gradually decreases with increasing NGHS, whereas increases with increasing confining pressure. In addition, the proposed model has been applied to the 142.9–147.7 m well section of Well DK-1 in the permafrost region, Qilian Mountains. The results show that the error of the predicted results is less than 13.92%, with its average error being 10.51%. The predicted value gradually increases with its error decreasing as the depth continues to increase, which is consistent with the change behavior of measured data. NGHS evaluation method proposed in this paper fully considers the occurrence form of natural gas hydrate in reservoirs. The model parameters are easy to determine and the predicted results are reliable.

AbstractContext Polygonum hydropiper L. (Polygonaceae) (PH) is a traditional Chinese traditional medicine with a pungent flavor and mild drug properties. PH is mainly distributed in the channel tropism in the stomach and large intestine. PH has multiple uses and can be used to treat a variety of diseases for a long time.Objective This review summarizes the phytochemical and pharmacological activities, and applications of PH from 1980 to 2022. We also provide suggestions for promoting further research and developing additional applications of PH.Methods The data and information on PH from 1980 to 2022 reviewed in this article were obtained from scientific databases, including Science Direct, PubMed, Science Citation Index, SciFinder Scholar (SciFinder), Springer, American Chemical Society (ACS) Publications, and China National Knowledge Infrastructure (CNKI), etc. Some information was obtained from classic literature on traditional Chinese medicines. The search terms were Polygonum hydropiper, phytochemistry compositions of Polygonum hydropiper, pharmacological activities of Polygonum hydropiper, and applications of Polygonum hydropiper.Results The comprehensive analysis of the literature resulted in 324 compounds being isolated, identified, and reported from PH. Regarding traditional uses, the majority of phytochemical and pharmacological studies have indicated the diverse bioactivities of PH extracts, flavonoids, and volatile oil elements, including antibacterial, antifungal, insecticidal, antioxidant, and anti-inflammatory.Conclusions PH has a long history of diversified medicinal uses, some of which have been verified in modern pharmacological studies. Further detailed studies are required to establish scientific and reasonable quality evaluation standards and action mechanisms of active constituents from PH.

AbstractContext Schisandrin B (Sch B), an active ingredient from Schisandrae chinensis (Turcz.) Baill. (Schisandraceae) Fructus, possesses diverse pharmacological activities including antitumor, anti-inflammation, and hepatoprotection.Objective To explore the effect of Sch B on activated HSCs senescence in hepatic fibrosis and the mechanisms implicated.Materials and methods ICR mice with CCl4-induced hepatic fibrosis were supplemented with Sch B (40 mg/kg) for 30 d and LX2 cells were treated with Sch B (5, 10 and 20 μM) for 24 h. Cellular senescence was assessed by senescence-related indicators senescence-associated β-galactosidase (SA-β-gal) activity and the expression of p16, p21, p53, γ-H2AX, H3K9me3, TERT, TRF1, and TRF2. Ferric ammonium citrate (FAC) and NCOA4 siRNA were used to evaluate the mechanisms underlying Sch B’s regulation of cellular senescence.Results Sch B (40 mg/kg) reduced serum levels of AST and ALT (53.2% and 63.6%), alleviated hepatic collagen deposition, and promoted activated HSCs senescence in mice. Treatment with Sch B (20 μM) decreased cell viability to 80.38 ± 4.87% and elevated SA-β-gal activity, with the levels of p16, p21 and p53 increased by 4.5-, 2.9-, and 3.5-fold and the levels of TERT, TRF1 and TRF2 decreased by 2.4-, 2.7-, and 2.6-fold in LX2 cells. FAC (400 μM) enhanced Sch B’s effect mentioned above. NCOA4 siRNA weakened the effects of Sch B on iron deposition and HSCs senescence.Conclusions Sch B could ameliorate hepatic fibrosis through the promotion of activated HSCs senescence, which might be attributed to its induction of NCOA4-mediated ferritinophagy and subsequent iron overload.

AbstractContext Our previous studies have found that total flavonoid of Alpinia officinarum Hance (Zingiberaceae) (F.AOH) had protective effects on gastric ulcer (GU).Objective To investigate the protective mechanism of F.AOH on acetic acid-induced chronic GUs in rats and ethanol-induced GES-1 cells damage.Materials and methods In vivo: Gastric damage was induced in SD rats by administering acetic acid after oral treatment with F-AOH at 54, 27 and 13.5 mg/kg (2 weeks of continuous gavage). After a comprehensive evaluation of rats’ serum and gastric tissue-related indicators, gene transcriptome sequencing, qPCR and Western blotting were used to investigate the mechanism further. In vivo: GES-1 cells were incubated with F-AOH (8, 4 and 2 μg/mL) for 16 h and treated with 7% ethanol for 4 h. Transwell and flow cytometry were employed to detect migration and apoptosis of cells.Results F.AOH effectively reduced the area of GUs in rats (from 11.2 ± 1.89 to 2.19 ± 0.95), reversing ethanol-induced cells apoptosis (from 23 ± 1.3 to 8.11 ± 0.93%). It also inhibited the expression of endothelin-1 (ET-1) and iNOS proteins, decreasing the levels of TNF-α IL-6 in serum, improving oxidative stress levels and increasing the expression of Bcl-2/Bax dimer genes. In addition, 4005 differentially expressed genes between the acetic acid model and the drug groups. Through experimental verification, F.AOH can inhibit the activation of TLR4/NF-κB signalling pathway and TRPV1 receptor.Conclusions F.AOH, as an effective gastric protective plant component, had potential therapeutic value in anti-inflammatory pain and antioxidative stress gastrointestinal diseases.

There is limited research on the relationship between membrane fouling and microbial metabolites in the nitrogen removal process coupled with membrane bioreactors (MBRs). In this study, we compared anoxic-oxic (AO) and partial nitritation–anammox (PNA), which were selected as representative heterotrophic and autotrophic biological nitrogen removal–coupled MBR processes for their fouling behavior. At the same nitrogen loading rate of 100 mg/L and mixed liquor suspended solids (MLSS) concentration of 4000 mg/L, PNA-MBR exhibited more severe membrane fouling compared to AO-MBR, as evidenced by monitoring changes in transmembrane pressure (TMP). In the autotrophic nitrogen removal process, without added organic carbon, the supernatant of PNA-MBR had higher concentrations of protein, polysaccharides, and low-molecular-weight humic substances, leading to a rapid flux decline. Extracellular polymeric substances (EPS) extracted from suspended sludge and cake sludge in PNA-MBR also contributed to more severe membrane fouling than in AO-MBR. The EPS subfractions of PNA-MBR exhibited looser secondary structures in protein and stronger surface hydrophobicity, particularly in the cake sludge, which contained higher contents of humic substances with lower molecular weights. The higher abundances of Candidatus Brocadia and Chloroflexi in PNA-MBR could lead to the production of more hydrophobic organics and humic substances. Hydrophobic metabolism products as well as anammox bacteria were deposited on the hydrophobic membrane surface and formed serious fouling. Therefore, hydrophilic membrane modification is more urgently needed to mitigate membrane fouling when running PNA–MBR than AO–MBR.

In this study, the effects of variations in the height (h) and bottom radius (r) of black silicon microstructures on their absorptance and photoelectric response efficiency were analyzed. By using the relation cot⁡θ2=hr to combine the parameters, it was found that changes in morphology affected the absorptance of black silicon microstructures, with h being directly proportional to the absorptance, while r was inversely proportional. A positive correlation was observed between cot⁡θ2 and absorptance. However, the correlation between cot⁡θ2 and photoelectric response efficiency was not significant. Through Raman spectroscopy analysis of the samples, it was concluded that as the laser ablation energy density increased, more lattice defects were introduced, weakening the charge carrier transport efficiency. This study further elucidated the mechanism by which microstructural changes impacted the absorptance and energy density of black silicon, providing valuable insights for optimizing its energy density.

Controlling the total amount of river pollutant discharge is an important means of water resource protection and management, and it is also a necessary condition for ensuring the normal functioning of water areas. The total amount of pollutant discharge is closely related to the water environmental capacity (WEC). Shifting from the traditional method of calculating WEC to dynamic analyses and calculations, concerning practical applications, in this paper, a dynamic adaptive calculation method is proposed for the river WEC that considers the changes in adaptive demand and hydrological conditions. In this method, the dynamic WEC is represented by intervals based on dynamic changes in different spatial and temporal scales, various calculation methods, hydrological conditions, and parameters. According to the calculation results for the WEC, a variable interval was formed. Taking the Shaanxi section of the main stream of the Wei River as the research object, with the support of an integrated platform, the dynamic adaptive calculation of the WEC in the Shaanxi section of the Wei River was realized, and a corresponding simulation system was constructed. The verification results show that (1) the dynamic calculation of WEC can be realized by freely combining different model methods and calculation conditions; (2) the WEC is described using a variable interval, which has strong applicability and operability; and (3) the simulation system can quickly adapt to the changing needs of practical applications and provide managers with visual and credible decision support. The research results provide a theoretical basis for river water environment pollution prevention and environmental management decision-making and help in the high-quality development of the river basin.

Abstract Tropical cyclones (TCs) over the Bay of Bengal (BOB) can interact with the South Branch Trough (SBT) as they move northward and potentially amplify Rossby waves. This study evaluates the features of Rossby waves and their downstream impact on rainfall in South China. Results indicate that TC‐SBT interactions primarily occur in May and October‐November (Oct‐Nov), with probabilities of 59% and 53% respectively. Notably, the Rossby wave train associated with BOB TCs is more pronounced during Oct‐Nov due to the stronger subtropical westerly jet, in contrast to May. The downstream atmospheric response results in positive (negative) rainfall anomalies over South China in May (Oct‐Nov), particularly on the day following the maximum interaction day. Previous researches concerning TC‐extratropical flow interaction mainly focus on other basins where TCs move to higher latitudes, this study provides fresh insights into Rossby waves related to TC‐SBT interactions over the southern Tibetan Plateau.

BackgroundGender disparities in mortality have drawn great interest, with previous studies identifying various biological, social, and behavioral factors contributing to the observed gender differences. This study aims to identify the sources of gender disparities in mortality rates and quantify the extent to which these factors mediate the gender differences in all-cause mortality.MethodsData from the National Health and Nutrition Examination Survey (NHANES) conducted between 2005 and 2018 were analyzed. A total of 38,924 participants were included in the study. Gender information, socioeconomic status, lifestyle factors, and baseline disease status were obtained through questionnaires. Blood samples were collected to assess serological indicators. All-cause and cardiovascular mortality were considered as primary and secondary outcomes, respectively.ResultsThe study with an average age of 50.1 ± 17.9 years. Among the participants, 50.7% were women, and 41.8% were non-Hispanic White. The median follow-up length was 87 months [Inter-Quartile Range (IQR): 47–128]. Men showed higher rates of all-cause and cardiovascular mortality compared to women in both the general population and the population with cardiovascular disease. After adjustment for potential confounders (age, race, marital status, socioeconomic status, lifestyle level, smoking status, cardiovascular disease, hypertension, diabetes and cancer), the men: women hazard ratios (HRs) for all-cause and cardiovascular mortality were 1.58 [95% Confidence Interval (CI): 1.48–1.68] and 1.60 (95%CI:1.43–1.80) in the general population. Among individuals with cardiovascular disease, the fully adjusted HR for all-cause mortality was 1.34 (95% CI: 1.20 to 1.51), and for cardiovascular mortality, the fully adjusted HRs was 1.52 (95% CI: 1.26 to 1.83). Mediation analysis revealed that uric acid levels significantly mediated the association between gender and all-cause mortality, accounting for 17.53% (95% CI: 11.0% to 23.7%) in the general population and 27.47% (95% CI: 9.0% to 13.6%) in the population with cardiovascular disease.ConclusionsThe study highlights the complex interplay of biological and social factors contributing to gender disparities in mortality. Uric acid was identified as key mediators of the gender-mortality association. These findings can inform targeted interventions aimed at reducing gender disparities in mortality and promoting better public health outcomes.

Background: Application of individualized positive end-expiratory pressure (PEEP) based on minimum driving pressure facilitates to prevent from postoperative pulmonary complications (PPCs). Whether lung protective ventilation strategy can reduce the risk of PPCs in COVID-19 patients remains unclear. In this study, we compared the effects of driving pressure-guided ventilation with conventional mechanical ventilation on PPCs in patients with COVID-19. Methods: Patients infected COVID-19 within 30-day before surgery were retrospectively enrolled consecutively. Patients were divided into two group: driving pressure-guided lung protective ventilation strategy group (LPVS group) and conventional mechanical ventilation group (Control group). Propensity score matching for variables selected was used by logistic regression with the nearest-neighbor method. The outcomes were the incidence of PPCs and hypoxemia in post-anesthesia care unit. Results: There was no significant difference in the baseline data between both groups (P > 0.05). The incidence of PPCs (12.73 % vs 36.36 %, χ2 = 7.068, P = 0.008) and hypoxemia [18.18 % vs 38.18 %, χ2 = 4.492, P = 0.034], and lung ultrasound scores [4.68 ± 1.60 vs 8.39 ± 1.87, t = 8.383, P

Abstract Background Biological ageing is tightly linked to cardiovascular disease (CVD). We aimed to investigate the relationship between Life’s Essential 8 (LE8), a currently updated measure of cardiovascular health (CVH), and biological ageing. Methods This cross-sectional study selected adults ≥ 20 years of age from the 2005–2010 National Health and Nutrition Examination Survey. LE8 scores (range 0–100) were obtained from measurements based on American Heart Association definitions, divided into health behavior and health factor scores. Biological ageing was assessed by different methods including phenotypic age, phenotypic age acceleration (PhenoAgeAccel), biological age and biological age acceleration (BioAgeAccel). Correlations were analyzed by weighted linear regression and restricted cubic spline models. Results Of the 11,729 participants included, the mean age was 47.41 ± 0.36 years and 5983 (51.01%) were female. The mean phenotypic and biological ages were 42.96 ± 0.41 and 46.75 ± 0.39 years, respectively, and the mean LE8 score was 67.71 ± 0.35. After adjusting for potential confounders, higher LE8 scores were associated with lower phenotypic age, biological age, PhenoAgeAccel, and BioAgeAccel, with nonlinear dose–response relationships. Negative associations were also found between health behavior and health factor scores and biological ageing, and were stronger for health factors. In health factor-specific analyses, the β negativity was greater for blood glucose and blood pressure. The inverse correlations of LE8 scores with phenotypic age and biological age in the stratified analyses remained solid across strata. Conclusions LE8 and its subscale scores were strongly negatively related to biological ageing. Encouraging optimal CVH levels may be advantageous in preventing and slowing down ageing.

Phenotypic variation analysis and comprehensive evaluation are important tools for selecting provenances of forest trees, which, in turn, is crucial for genetic improvement of forest trees. The study analyzed eight needle-leaf and fourteen tracheid trait indexes of ten provenances of Qinghai spruce forest stands. Multiple comparisons, correlation analysis, cluster analysis, and principal component analysis were used to evaluate the differences in needle-leaf and tracheid traits among the different provenances. The study found significant differences in the needle-leaf and tracheid traits among the provenances. All traits included 50 pairs of positive and 26 pairs of negative correlations. The coefficient of variation for the tracheid trait (18.86%) was higher than that for the needle-leaf trait (17.20%). A comprehensive evaluation of needle-leaf and tracheid traits was conducted using principal component analysis. The study demonstrated that the degree of variation in needle-leaf and tracheid traits among the provenances of Qinghai spruce was extensive, with a multitude of sources of variation. Therefore, it is crucial to strengthen the selection of good provenances in the early seed selection process. The results of the comprehensive evaluation can be utilized as a foundation for the selection of good provenances for carbon sequestration and timber properties.

Abstract Guillain-Barré syndrome (GBS) is an acute idiopathic polyneuropathy which is related to infection and immune mechanism. The exact pathogenesis of the disease is unknown and treatment is limited. Thus, the purpose of the study is to identify biomarkers of GBS serum and elucidate their involvement in the underlying pathogenesis of GBS that could help to treat GBS more accurately. Antibody array technology was used to detect the expression levels of 440 proteins in serum of 5 GBS group and 5 healthy control group. Sixty-seven differentially expressed proteins (DEPs) were identified by antibody array, among which FoLR1, Legumain, ErbB4, IL-1α, MIP-1α and IGF-2 were down-regulated, while 61 proteins were up-regulated. Bioinformatics analysis indicated that most DEPs were associated with leukocytes, among which IL-1α, SDF-1b, B7-1, CD40, CTLA4, IL-9, MIP-1α and CD40L were in the center of protein–protein interaction (PPI) network. Subsequently, the ability of these DEPs to distinguish GBS from healthy control was further evaluated. CD23 was identified by means of Random Forests Analysis (RFA) and verified by enzyme-linked immunosorbent assay (ELISA). The ROC curve result of CD23 respectively displayed that its sensitivity, specificity and AUC were 0.818, 0.800 and 0.824. We speculate that activation of leukocyte proliferation and migration in circulating blood might be associated with inflammatory recruitment of peripheral nerves, leading to the occurrence and development of GBS, but this conclusion still requires deeper confirmation. More importantly, central proteins may play a pivotal role in the pathogenesis of GBS. In addition, we detected IL-1α, IL-9, and CD23 in the serum of GBS patients for the first time, which may be promising biomarkers for the treatment of GBS.

Abstract This study investigates the interannual variability of rapid intensification (RI) magnitude of western North Pacific (WNP) tropical cyclones. There is a significant correlation between basin‐averaged RI magnitude during July–November and the simultaneous Western Pacific (WP) teleconnection index from 1982 to 2021. RI magnitude is, on average, larger (smaller) in positive (negative) WP phases. During a positive WP, RI magnitude changes exhibit a southwest‐northeast dipolar pattern, with significant increases over the southwestern quadrant of the WNP and weak decreases over the northern part of the WNP. The WP teleconnection relates to RI magnitude primarily through modulation of 850–200‐hPa vertical wind shear, with less influence from 850‐hPa relative vorticity. The changes in these two dynamic conditions can be linked to WP‐induced circulation anomalies at lower and upper levels. Our results highlight that different contributors may be responsible for changes in WNP RI occurrence and RI magnitude.

Purpose: To evaluate the survival benefit of radiation plus chemotherapy in adult females with stage IIIC endometrial cancer and to investigate whether the benefit varies according to histology. Methods: Data from adult females with International Federation of Gynecology and Obstetrics (FIGO) stage IIIC endometrial cancer, who underwent at least total hysterectomy between 2010 and 2015, were obtained from the Surveillance, Epidemiology, and End Results (SEER) database. Adjuvant treatments were categorized as chemotherapy alone, chemotherapy with external beam radiation therapy (EBRT), chemotherapy with vaginal brachytherapy (VBT), or chemotherapy with EBRT+VBT. Multivariate Cox regression models, Kaplan–Meier curves, and log-rank tests were used to assess the association between treatment modality and overall survival (OS). Results: In total, 2138 cases were identified: stage IIIC1 (n = 1299 [60.8%]) and stage IIIC2 (n = 839 [39.2%]). Median OS for all patients was 48 (interquartile range [IQR] 28-70) months. Regarding adjuvant treatment, 40.5% of patients underwent chemotherapy only, followed by chemotherapy with EBRT (35.5%). Stage IIIC patients treated with chemotherapy plus radiation exhibited a significantly reduced risk for death from endometrial cancer in both univariate and multivariate analyses ( P

A case of trichilemmal cysts is reported. A 31-year-old man presented with a 5-year history of nodule on his back with enlargement, pain and itching for 1 month. Dermatological examination showed erythema and erythematous plaques scattered on the trunk and extremities, covered with a small amount of silvery white scales. A demarcated exophytic tumor sized 2 cm×3 cm in dark red color was seen on the back, with telangiectasia on the surface. The tumor was slightly soft and tenderness (+) with poor mobility. No erosion or ulceration was observed. Histopathological examination showed a dermal cyst, which wall was composed of stratified squamous cells, and the content of cyst was keratin with focal calcification. The patient was diagnosed with trichilemmal cyst. Complete resection was given. No recurrence was observed over 4 months of follow-up.

Objective To analyze the correlations between plasma orexin⁃A and clinical manifestations of dementia with Lewy bodies (DLB). Methods A total of 51 patients with DLB from Beijing Tiantan hospital, Capital Medical University, and 46 sex, age and education matched controls were conducted from January 2019 to December 2021. Overall cognitive function was assessed by Mini⁃Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA), dementia severity was assessed by Clinical Dementia Rating Scale (CDR), activities of daily living was assessed by Activities of Daily Living Scale (ADL), depression severity was assessed by Hamilton Depression Rating Scale 17 (HAMD⁃17), neuropsychiatric symptoms was assessed by Neuropsychiatric Inventory (NPI), and plasma orexin⁃A level was measured. The univariate and multivariate Logistic regression analyses were used to screen influencing factors for DLB. Pearson and partial correlation analyses were used to explore the correlation between plasma orexin⁃A and core clinical features of DLB. Multivariate linear stepwise regression analysis was used to analyze the linear correlation between plasma orexin⁃A level and neuropsychological tests. Results The scores of MMSE (Z=⁃ 8.387, P=0.000) and MoCA (Z=⁃8.479, P=0.000) in DLB group were significantly lower than those in control group, while the scores of CDR (Z=⁃9.072, P = 0.000), ADL(Z=⁃8.692, P=0.000), HAMD⁃17 (Z=⁃7.568, P=0.000), NPI (Z=⁃8.270, P=0.000) and plasma orexin⁃A level (Z=⁃2.688, P=0.007) were significantly higher than those in control group. Plasma orexin⁃A level in DLB patients with fluctuating cognition (Z=⁃2.172, P=0.030) and with Parkinsonism (Z=⁃1.981, P=0.048) were lower than those without these symptoms, respectively. Logistic regression analysis showed drinking history was a potentially independent protective factor for DLB (OR=0.278, 95%CI:0.095-0.808; P=0.019), while increased plasma orexin⁃A level was an independent risk factor for DLB (OR=6.878, 95%CI:1.241-38.137; P=0.027). Correlation analysis showed that plasma orexin⁃A level was negatively correlated with the occurrence of Parkinsonism in patients with DLB (r=⁃ 0.322, P=0.043). Multivariate linear stepwise regression analysis showed no statistically significant relationship between the plasma orexin⁃A level and the scores of neuropsychological tests (P>0.05, for all). Conclusions The plasma orexin⁃A level in patients with DLB were higher than controls, and the elevated plasma orexin⁃A level could significantly increase the risk of DLB, while reduce the incidence of Parkinsonism. Dysfunction of orexin⁃A system might be a potential mechanism for the occurrence of DLB.

Abstract Background As a common malignant tumor, esophageal carcinoma (ESCA) has a low early diagnosis rate and poor prognosis. This study aimed to construct the prognostic features composed of ZNF family genes to effectively predict the prognosis of ESCA patients. Methods The mRNA expression matrix and clinical data were downloaded from TCGA and GEO database. Using univariate Cox analysis, lasso regression and multivariate Cox analysis, we screened six prognosis-related ZNF family genes to construct the prognostic model. We then used Kaplan-Meier plot, time-dependent receiver operating characteristic (ROC), multivariable Cox regression analysis of clinical information, and nomogram to evaluate the prognostic value within and across sets, separately and combined. We also validated the prognostic value of the six-gene signature using GSE53624 dataset. The different immune status was observed in the single sample Gene Set Enrichment Analysis (ssGSEA). Finally, real-time quantitative PCR was used to detect the expression of six prognostic ZNF genes in twelve pairs of ESCA and adjacent normal tissues. Results A six prognosis-related ZNF family genes model consisted of ZNF91, ZNF586, ZNF502, ZNF865, ZNF106 and ZNF225 was identified. Multivariable Cox regression analysis revealed that six prognosis-related ZNF family genes were independent prognostic factors for overall survival of ESCA patients in TCGA and GSE53624. Further, a prognostic nomogram including the riskScore, age, gender, T, stage was constructed, and TCGA/GSE53624-based calibration plots indicated its excellent predictive performance. Drug Sensitivity and ssGSEA analysis showed that the six genes model was closely related to immune cells infiltration and could be used as a potential predictor of chemotherapy sensitivity. Conclusion We identified six prognosis-related ZNF family genes model of ESCA, which provide evidence for individualized prevention and treatment.

Objective To investigate the effects of β-estradiol (E2) on the phosphorylation of JAK/STAT, PI3K/AKT signaling pathways and the expression of IL-17A in mouse spleen mononuclear cells treated with IL-36β. Methods The spleens were isolated from female SPF C57BL/6 mice aged 6-8 weeks. The mononuclear cell suspensions were obtained from spleens by density gradient centrifugation. The suspensions of mononuclear cells were transferred into four petri dishes, with 1.5×107 cells/dish, followed by the treatment with either E2 (10-8 mol/L) or IL-36β (10 ng/mL) or IL-36β+E2, while untreated cells served as normal controls. Western blotting was used to assess the changes in phosphorylation of JAK/STAT and PI3K/AKT proteins 30 min after incubation with IL-36β or E2. Moreover, the mononuclear cells were also treated with IL-36β, E2, JAK inhibitor and PI3K inhibitor, respectively, followed by assessment of IL-17A mRNA levels at 6, 12 and 24 h, meanwhile expression levels of IL-17A protein were measured with Western blot at 24 h. Results IL-36β activated the phosphorylation of JAK3/STAT2 and PI3K/AKT in mouse spleen mononuclear cells, while E2 inhibited such activation induced by IL-36β. Expression levels of IL-17A mRNA differed significantly among four groups of cells at 6, 12 and 24 h (F=25.50, 127.04 and 9.76, respectively, all P

Abstract The long-term use of chemical fertilizers to maintain agricultural production has had various harmful effects on farmland and has greatly impacted agriculture's sustainable expansion. Graphene, a unique and effective nanomaterial, is used in plant-soil applications to improve plant nutrient uptake, reduce chemical fertilizer pollution by relieving inadequate soil nutrient conditions and enhance soil absorption of nutrient components. We investigated the effects of graphene amendment on nutrient content, maize growth, and soil physicochemical parameters. In each treatment, 5 graphene concentration gradients (0, 25, 50, 100, and 150 g kg−1) were applied in 2 different types (single-layer and few-layers, SL and FL). Soil aggregates, soil accessible nutrients, soil enzyme activity, plant nutrients, plant height, stem diameter, dry weight, and fresh weight were all measured throughout the maize growth to the V3 stage. Compared to the control (0 g kg−1), we found that graphene increased the percentage of large agglomerates (0.25–10 mm) in the soil and significantly increased the geometric mean diameter (GMD) and mean weight diameter (MWD) values of > 0.25 mm water-stable agglomerates as the increase of concentration. Soil available nutrient content (AN, AP, and AK) increased, peaking at 150 g kg−1. Graphene boosted nutrient absorption by maize plants, and aboveground total nitrogen (TN), total phosphorus (TP), and total potassium (TK) contents rose with the increasing application, which raised aboveground fresh weight, dry weight, plant height, and stalk thickness. The findings above confirmed our prediction that adding graphene to the soil may improve maize plant biomass by enhancing soil fertility and improving the soil environment. Given the higher manufacturing cost of single-layer graphene and the greater effect of few-layer graphene on soil and maize plants at the same concentration, single-layer graphene and few-layer graphene at a concentration of 50 g kg−1 were the optimal application rates.

The parallel reservoirs in the upper reach of the Hanjiang River are key projects for watershed management, development, and protection. The optimal operation of parallel reservoirs is a multiple-stage, multiple-objective, and multiple-decision attributes complex decision problem. Taking Jiaoyan–Shimen parallel reservoirs as an example, a method of multi-objective optimal operation decision of parallel reservoirs (MOODPR) was proposed. The multi-objective optimal operation model (MOOM) was constructed. The new algorithm coupling NSGA-II, TOPSIS, and GCA was used to solve the MOODPR problem. The method of MOODPR was formed by coupling problem identification, model construction, an optimization solution, and scheme evaluation. The results show that (1) combining the Euclidean distance with the grey correlation degree to construct a new hybrid closeness degree makes the multi-attribute decision making method more scientific and feasible. (2) The NSGA-II-TOPSIS-GCA algorithm is applied to obtain decision schemes, which provide decision support for management. (3) It can be seen from the Pareto chart that for the Jiaoyan–Shimen parallel reservoirs, the comprehensive water supply was negatively related to ecology. (4) The comprehensive water supply and ecological AAPFD value in the extraordinarily dry year was 4.212 × 108 m3 and 4.953. The number of maximum continuous water shortage periods was 4 and 6. The maximum ten-day water shortage was 4.46 × 107 m3 and 2.3 × 106 m3. The research results provide technical support and reference value to multi-objective optimal operation decisions for parallel reservoirs in the upper reach of the Hanjiang River.

The use of edible packaging films to delay food spoilage has attracted widespread attention. In this study, partridge tea extract (PTE) was added to cassia gum (CG) to prepare CG/PTE films. The microstructure, optical, mechanical, barrier, and antioxidant properties of CG/PTE films were investigated, and the effect of PTE on CG films was shown. The films had high transparency and smooth surface structure. Additionally, PTE significantly improved the elongation at break and antioxidant activity of films. At 2.5% of PTE, the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging rate of the film was 46.88% after diluting 50 times, indicating excellent antioxidant property, which could be applied to food preservation. After 9 days of storage, the thiobarbituric acid reactive substances values (TBARS) of chicken jerk packaged with films containing 0% and 2.5% PTE increased from 0.12% to 1.04% and 0.11% to 0.40%, respectively. This study suggests that CG/PTE films can be used to preserve cooked meat.

The CXCR2 chemokine receptor is known to have a significant impact on the initiation and control of inflammatory processes. However, its specific involvement in the sensation of itch is not yet fully understood. In this study, we aimed to elucidate the function of CXCR2 in the trigeminal ganglion (TG) by utilizing orofacial itch models induced by incision, chloroquine (CQ), and histamine. Our results revealed a significant up-regulation of CXCR2 mRNA and protein expressions in the primary sensory neurons of TG in response to itch stimuli. The CXCR2 inhibitor SB225002 resulted in notable decrease in CXCR2 protein expression and reduction in scratch behaviors. Distal infraorbital nerve (DION) microinjection of a specific shRNA virus inhibited CXCR2 expression in TG neurons and reversed itch behaviors. Additionally, the administration of the PI3K inhibitor LY294002 resulted in a decrease in the expressions of p-Akt, Akt, and CXCR2 in TG neurons, thereby mitigating pruritic behaviors. Collectively, we report that CXCR2 in the primary sensory neurons of trigeminal ganglion contributes to orofacial itch through the PI3K/Akt signaling pathway. These observations highlight the potential of molecules involved in the regulation of CXCR2 as viable therapeutic targets for the treatment of itch.

Alkaline ferrous slags pose global environmental issues and long-term risks to ambient environments. To explore the under-investigated microbial structure and biogeochemistry in such unique ecosystems, combined geochemical, microbial, ecological and metagenomic analyses were performed in the areas adjacent to a ferrous slag disposal plant in Sichuan, China. Different levels of exposure to ultrabasic slag leachate had resulted in a significant geochemical gradient of pH (8.0–12.4), electric potential (−126.9 to 437.9 mV), total organic carbon (TOC, 1.5–17.3 mg/L), and total nitrogen (TN, 0.17–1.01 mg/L). Distinct microbial communities were observed depending on their exposure to the strongly alkaline leachate. High pH and Ca2+ concentrations were associated with low microbial diversity and enrichment of bacterial classes Gamma-proteobacteria and Deinococci in the microbial communities exposed to the leachate. Combined metagenomic analyses of 4 leachate-unimpacted and 2-impacted microbial communities led to the assembly of one Serpentinomonas pangenome and 81 phylogenetically diversified metagenome assembled genomes (MAGs). The prevailing taxa in the leachate-impacted habitats (e.g., Serpentinomonas and Meiothermus spp.) were phylogenetically related to those in active serpentinizing ecosystems, suggesting the analogous processes between the man-made and natural systems. More importantly, they accounted for significant abundance of most functional genes associated with environmental adaptation and major element cycling. Their metabolic potential (e.g., cation/H+ antiporters, carbon fixation on lithospheric carbon source, and respiration coupling sulfur oxidization and oxygen or nitrate reduction) may support these taxa to survive and prosper in these unique geochemical niches. This study provides fundamental understandings of the adaptive strategies of microorganisms in response to the strong environmental perturbation by alkali tailings. It also contributes to a better comprehension of how to remediate environments affected by alkaline industrial material.

Abstract The international pesticide trade network (iPTN) is a key factor affecting global food production and food security. The trade relationship is a key component in iPTNs. In a complex international trade environment, we model the impacts of uncertain factors such as trade wars, economic blockades and local wars, as removing vital relationships in the trade network. There are many complex network studies on node centrality, but few on link centrality or link importance. We propose a new method for computing network link centrality. The main innovation of the method is in converting the original network into a dual graph, the nodes in the dual graph corresponding to the links of the original network. Through the dual graph, the node centrality indicators can measure the centrality of the links in the original network. We verify the effectiveness of the network link centrality indicator based on the dual graph in the iPTN, analyze the relationship between the existing network link centrality indicators and the indicator proposed in this paper, and compare their differences. It is found that the trade relationships with larger indicators (hub, outcloseness, outdegree) based on the dual graph have a greater impact on network efficiency than those based on the original pesticide trade networks.

Abstract This study aimed to investigate the effects of early-stage Alzheimer’s disease (AD) on the reach-to-grasp kinematics and kinetics with and without visual supervision of the grasping arm and hand. Seventeen patients who had been diagnosed with early-stage AD and 17 age- and gender-matched, cognitive normal (CN) adults participated in the experiment. A mirror operating system was designed to block the visual feedback of their grasping hand and forearms but to virtually show grasped targets. The target for reach-to-grasp kinematics was a reflective marker installed on a base; and the target for reach-to-grasp kinetics was a custom-made apparatus installed with two six-component force/torque transducers. Kinematics and kinetic parameters were used to quantify the reach-to-grasp performances. Results showed that the early-stage AD remarkably decreased the reaching speed, reduced the grasping accuracy and increased the transportation variability for reach-to-grasp kinematics. For kinetic analysis, early-stage AD extended the preload duration, disturbed the grip and lift forces coordination, and increased the feedforward proportion in the grasping force control. The AD-related changes in the reach-to-grasp kinematic and kinetic parameters depended on visual feedback and were associated with nervous system function according to correlation analyses with the neuropsychological testing. These results suggest that the abnormal kinematic and kinetic characteristics may correlate with the neuropsychological status of early-stage AD, and that the reach-to-grasp kinematic and kinetic maneuver could potentially be used as a novel tool for non-invasive screening or evaluation of early-stage AD.

A clear understanding of multiphase flow with phase change is of great significance in productivity evaluation, process optimization, and production analysis, which is one of the focus issues of underbalanced drilling. In the process of underbalanced drilling, the annular flow is very complex. To drill safely and protect the reservoir, the dynamic separation process of dissolved natural gas and the characteristics of downhole multiphase flow are studied in this paper. Focusing on the characteristics of live crude oil and physical parameters in the wellbore, the authors creatively consider the influence of the velocity on the solubility and establish a mathematical model of multi-physical field non-equilibrium phase change and multiphase flow considering velocity, temperature and pressure. According to the established mathematical model, the solubility diagram of natural gas is drawn, and the evolution law of flow parameters is analyzed. The results show that the influence of the three factors on solubility is that the influence of pressure is greater than that of temperature, and the influence of temperature is greater than that of flow velocity. The effect of flow velocity on annular pressure, temperature and phase content does not seem to be very great in the natural gas solubility model. However, the dissolved gas in the wellbore separates earlier when considering the velocity than when the velocity is not considered. It is concluded that the research presented here confirms that the influence of flow velocity cannot be ignored. The innovative mathematical model in this paper takes into account the effect of flow velocity on non-equilibrium multiphase flow with phase change. By using the model developed, a better understanding can be obtained of what the phase change characteristics of downhole fluid are and how the multiphase flow laws will change, which is of great significance to reservoir protection and drilling safety.

Accurate forecasting of energy consumption in office buildings is of great importance for optimal management of energy consumption and reduction of building energy consumption. A variety of combination forecasting models (FMs) have become the current research hotspots in the field of building energy consumption forecasting. For the problems of large systematic errors and poor generalization ability of existing combination FMs, this paper proposes a dynamic combination residual forecasting model (FM) with the optimal combination approach. Firstly, support vector regression (SVR) is selected as the basic FM, and the SVR residual errors are forecasted by the dynamic combination FM based on the weights, and the SVR forecast value is finally corrected. Further, the basis for the selection of the single FM in the combination model and the optimal number of combination terms are given by mathematical proof in this paper. A case study in Xi’an shows that the dynamic combined residual errors correction FM with the optimal number of terms proposed in this paper can reduce the mean absolute error (MAE) of the basic model from 1918.59 kW to 349.37 kW, the mean absolute percentage error (MAPE) from 15.80% to 2.96%, and the root mean square error (RMSE) from 2278.74 to 471.44.

Objective To explore the effect of Treponema pallidum (Tp) -induced exosomes (EXOTp) by macrophages on macrophage polarization. Methods Human monocyte-macrophages (THP-1) were induced into macrophages, followed by stimulation with or without Tp for 24 h. After changing medium, cells were cultured for additional 48 h. The macrophage exosome suspension was collected, and exosomes were isolated by differential speed centrifugation. Isolated exosomes were identified by both Western blotting and transmission electron microscopy, and were quantitated with nanoparticle tracking technology. The internalization of the macrophages in the two groups was observed under a laser confocal microscope. In the experimental group, the isolated EXOTp was incubated with M0 macrophages for 24 h. Incubation of M0 macrophages with exosomes produced by non-Tp-treated macrophages served as negative controls, while M0 macrophages incubated without exosomes served as blank controls. At 6,12, and 24 h, the expression levels of INOS, IL-12, Arg-1, IL-10 and other mRNAs in all three groups were detected by qRT-PCR. Flow cytometry was used to identify macrophage phenotypes using MHC-Ⅱ, CD86, CD206, and CD209 markers. Results qRT-PCR showed that the expression levels of INOS and IL-12 mRNA in experimental group were time-dependently up-regulated (P0.05). Flow cytometry results showed that MHC-Ⅱ was positive (P0.05). Conclusions The exosomes secreted by THP-1-derived macrophages cannot induce the polarization of M0 macrophages to M1 macrophages. Upon the stimulation with Tp, the exosomes secreted by THP-1-derived macrophages can induce polarization of M0 macrophages to M1 macrophages.

A certain flow plays a key role in preventing the shrinkage and drying of the river while maintaining good functionality. In recent years, ecological baseflow has become an important research issue in China with the emergence of ecological protection. Various ecological baseflow methods and their improvement methods were proposed and applied, but the reliability and applicability of the results are yet to be verified. This paper proposes an advanced ecological baseflow computation-dynamic thematic evaluation service model by considering the ecological protection objectives. The method was applied to five key cross-sections of the Weihe River, and a set of recommended ecological baseflow values was obtained considering the connectivity, water environment, and water-sediment balance, which is in line with the actual requirements. The analysis shows that the method has reliability and operability, which can provide support for management decisions. HIGHLIGHTS Preselection of methods is positively helpful to the results’ reliability.; Applying multi-method approach in flow processes is more convincing than single ones.; The proposed correction can improve the simulation more close to practical requires.;

Abstract As part of the International GNSS Service (IGS) multi-GNSS Pilot Project (MGEX), precise orbit and clock products for multi-GNSS constellations have been submitted by several analysis centers (ACs) since 2012. Based on the 30 s satellite clocks from 6 MGEX ACs, the multi-GNSS clocks are combined and the consistency of the AC clocks is assessed in this study. Usually, a linear transformation between the combined and AC solution is used for clock combination, and the clock residuals of the AC solution w.r.t the combined solution are used to determine the weights of the AC. However, any inconsistent satellite-specific bias or nonlinear variations in the clocks induced by the AC’s processing strategy can contaminate the linear transformation as well as the determination of the weight. In this study, the analysis center and satellite-specific bias (ASB) of the MGEX AC clock solutions is first identified and estimated by using observations from globally distributed stations. Moreover, the clock solutions with nonlinear variations induced by the reference clock or nonconstant intersystem bias (ISB) are corrected by aligning the clocks to the selected reference solution before clock combination. With the correction of the ASB, the root-mean-square of the clock residuals decreases significantly and reaches 14 $$-$$ - 26, 37 $$-$$ - 91, 33 $$-$$ - 48 and 12 $$-$$ - 44 ps for GPS, GLONASS, BDS-2 and Galileo, respectively. In general, the consistency of AC solutions w.r.t the combination reaches 8 $$-$$ - 16, 27 $$-$$ - 58, 13 $$-$$ - 27 and 9 $$-$$ - 36 ps for GPS, GLONASS, BDS-2 and Galileo in terms of the standard deviation. Finally, the individual AC and combined orbit as well as clock solutions of different constellations are assessed by precise point positioning, and the combined multi-GNSS solutions show competitive performance with the best AC solution in terms of both the positioning accuracy and stability of the reference frame parameters. However, inconsistent scale parameters of both the AC and combined solutions are identified and require more investigation. Graphical Abstract

Water use of the ecosystem in the Wei River has been greatly reduced because of fierce competition between social–economic and eco-environmental water use, which leads to serious eco-environmental issues. Stakeholder theory was applied to the water resources utilization, and an improved holistic method for determining the eco-environmental flow was proposed. Considering the principles of inflow of tributaries, water intake from key sections, water balance and eco-environmental function regionalization, the new method integrated several methods to determine the water demand for the sub-items of eco-environmental flow. The three-level management targets of eco-environmental flow were proposed based on the opinions of stakeholders. Taking Shaanxi Section of the Wei River as an example, the eco-environmental flow at different levels was given for five key sections. The results show that: (1) the improved holistic method integrates multiple methods and models, making the calculation results of eco-environmental flow more scientific and reasonable; (2) the three-level management targets of eco-environmental flow can adapt to development and change in practical applications, and are operable and dynamic; (3) as a representative of the public benefits of ecosystems, government participates in the discussion of stakeholders and better coordinates competition among stakeholders. The improved method provides a new idea and reference for comprehensively and scientifically promoting reservoir ecological regulation. HIGHLIGHTS The improved holistic method combines various methods and models to make up for the limitation of a single method.; The three-level management targets of eco-environmental flow are operable and dynamic.; The government represents the public benefit of the ecosystem and better coordinates water use competition among stakeholders.;

Summary: Various stress conditions are signaled through phosphorylation of translation initiation factor eukaryotic initiation factor 2α (eIF2α) to inhibit global translation while selectively activating transcription factor ATF4 to aid cell survival and recovery. However, this integrated stress response is acute and cannot resolve lasting stress. Here, we report that tyrosyl-tRNA synthetase (TyrRS), a member of the aminoacyl-tRNA synthetase family that responds to diverse stress conditions through cytosol-nucleus translocation to activate stress-response genes, also inhibits global translation. However, it occurs at a later stage than eIF2α/ATF4 and mammalian target of rapamycin (mTOR) responses. Excluding TyrRS from the nucleus over-activates translation and increases apoptosis in cells under prolonged oxidative stress. Nuclear TyrRS transcriptionally represses translation genes by recruiting TRIM28 and/or NuRD complex. We propose that TyrRS, possibly along with other family members, can sense a variety of stress signals through intrinsic properties of this enzyme and strategically located nuclear localization signal and integrate them by nucleus translocation to effect protective responses against chronic stress.

The impact of the Northeast China Cold Vortex (NCCV) on tropical cyclone (TC) intensity change was studied based on statistics, revealing a significant decrease in the rate of TC weakening and a lower probability of rapid weakening (RW) in the environments of NCCVs. This is mainly attributable to the external dynamic forcing induced by the NCCV as the eddy angular momentum import at upper levels increases significantly, which offsets the unfavorable decrease in sea surface temperature and increase in vertical wind shear. The upper-level positive potential vorticity anomaly band from the NCCV involves into the TC circulation from the southwestern quadrant, helping the development of convections triggered in the downshear right side of the TC. These results elucidate a fundamental relationship between TCs and NCCVs, and the findings contribute to a deeper comprehension of TC behavior under the influence of NCCVs, thereby providing guidance for forecasting intensity changes of TCs entering mid-latitude regions.

Global processes and their teleconnections, such as the El Niño-Southern Oscillation (ENSO), have been shown to be a large driver of interannual changes in accumulated cyclone energy (ACE) of western North Pacific (WNP) tropical cyclones (TCs), with higher ACE during El Niño and lower ACE during La Niña. However, it remains uncertain whether interannual changes in WNP TC ACE are modulated by sea surface temperature anomalies (SSTAs) in other oceans. This study finds a significant negative correlation between WNP TC ACE during the early season (April–June) and simultaneous SSTAs over the tropical south Atlantic (TSA) in 1970–2021. On average, in warm TSA years, basinwide April–June ACE is significantly lower, with significant ACE decreases mainly occurring over the region spanning 5°–30°N, 115°–150°E. This is a result of reduced TC frequency, intensity and duration, due to a remote modulation of WNP environmental conditions by TSA SSTAs. In warm TSA years, there are significant decreases in 700–500-hPa relative humidity, 850-hPa relative vorticity and 200-hPa divergence and significant increases in 850–200-hPa vertical wind shear over the portion of the WNP with significant ACE reductions. These environmental changes can be linked to an anomalous Walker circulation induced by TSA SSTAs.

PSR J1012+5307 is a millisecond pulsar with an extremely low-mass white dwarf (WD) companion in an orbit of 14.5 hr. Magnetic braking (MB) plays an important role in influencing the orbital evolution of binary systems with a low-mass (≲1–2 M _⊙ ) donor star. At present, there exist several different MB descriptions. In this paper, we investigate the formation of PSR J1012+5307 as a probe to test plausible MB models. Employing a detailed stellar evolution model with the MESA code, we find that the convection- and rotation-boosted MB and “Intermediate” MB models can reproduce the WD mass, WD radius, WD surface gravity, neutron star mass, and orbital period observed in PSR J1012+5307. However, our simulated WD has a higher effective temperature than the observations. Three other MB mechanisms, including the standard MB model, are too weak to account for the observed orbital period in the Hubble time. A long cooling timescale caused by H-shell flashes of the WD may alleviate the discrepancy between the simulated effective temperature and the observed value.

The Yongfeng composite granitic pluton, located in the southern section of the Nanling area, is composed of the Yongfeng and Longshi biotite monzonitic granites. In order to reveal the genesis of this composite granitic pluton and its relationship with mineralization, this study conducted zircon U-Pb dating, whole-rock major and trace element analysis, and biotite electron probe analysis. The results show that the Yongfeng composite granitic pluton is rich in silicon and alkali, weakly peraluminous, and poor in calcium and iron. It shows the enrichment of light rare earth elements and a significant fractionation of light and heavy rare earth elements. It also shows the enrichment of large ion lithophile elements and depletion of Ba, K, P, Eu, and Ti relative to the primitive mantle. The contents of TFe2O3, MgO, CaO, TiO2, and P2O5 are low and decrease with increasing SiO2 content. The Yongfeng composite granitic pluton does not contain alkaline dark minerals. Its average zircon saturation temperature is 776 °C, average TFe2O3/MgO is 4.81, and average Zr + Nb + Ce + Y is 280.6 ppm, which correspond to a highly fractionated I-type granite. The Yongfeng and Longshi granites were respectively formed at 152.0 ± 1.0 Ma–151.3 ± 1.1 Ma and 148.9 ± 1.2 Ma. They were formed in the extensional tectonic setting during the post-orogenic stage, under the control of the breakup or retreat of the backplate after the subduction of the Pacific Plate into the Nanling hinterland. The magmatic system of the Yongfeng composite granitic pluton is characterized by high fractionation, high content of F, high temperature, and low oxygen fugacity, which is conducive to mineralization of Sn, Mo, and fluorite.

Functional vascular tree formation is a key step in many contexts, such as cancer, and Neuropilin1 (NRP1) has been associated with adhesion receptor endocytic turnover. Here, authors show NRP1 and its mini-WARS ligand play a role in reducing endothelial permeability.