The purpose of this study is to optimize the Green synthesis of AgNPs using various quantities (0.5, 0.75, 1, 1.25, 1.5, 1.75 ml) of Ruta leaf extracts, the biosynthesized AgNPs were characterized using several methods such as UV-Vis spectrum, FTIR spectroscopy, X-ray diffraction (XRD), FESEM, EDX analysis, DLS and zeta potential. The examination of the visible and ultraviolet spectra revealed an absorption peak in the region of 405–420 nm. Through the use of Fourier Transform Infrared (FTIR) spectroscopy, the functional groups that were found in the Ruta plant extract were analyzed in order to locate the components that were accountable for reducing the silver nitrate. Through XRD, which occurs in the form of (111) lattice planes in the face-centered cubic (FCC) structure of metallic silver, the biosynthesized AgNPs were a semispherical shape with a nanoscale size ranging between 30–50 nm and moderately stable at −22.53 mV and −25.88 mV. From the results of the tests, it was found from results that the optimum value for the extract volume is (1.5 ml) to show the optimal properties of silver nanoparticles. [ABSTRACT FROM AUTHOR]