1. Emerging microfluidic technologies for CRISPR-based diagnostics: an overview.
- Author
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Nafian F, Esfahani KS, Hobabi Aghmiuni M, Khoushab S, Illeslamllo T, Nafian S, Mohamadiyan N, Aleyasin NS, and Kamali Doust Azad B
- Abstract
In recent years, CRISPR (clustered regularly interspaced short palindromic repeats) has emerged as a detection technique with high specificity and sensitivity. However, it still needs improvements in terms of reducing cost, complexity, cross-contamination, technical requirements, and lack of quantification platforms. Microfluidic strategies can advance CRISPR-based technology and be modified to a higher level in the future. This review provides an overview of CRISPR-based detection systems (CRISPR-Dx) and their mechanism. Then, it explains how they have been optimized for fast and accurate point-of-care testing (POCT) using microfluidic devices such as SHINE, CARMEN, DNAiTECH, Dμchip, MAPnavi, FAST, and ITP. We discuss their innovations, primarily focusing on how they develop CRISPR-Dx in detection throughput, quantification, simple operation, visualization, sensitivity, specificity, and anti-contamination. more...
- Published
- 2025
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