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44 results on '"Nagao, Masaki"'

1. The Effects of Ninjinyoeito on Impaired Spatial Memory and Prefrontal Cortical Synaptic Plasticity through α-Amino-3-hydroxy-5-4-isoxazole Propionic Acid Receptor Subunit in a Rat Model with Cerebral Ischemia and β-Amyloid Injection

Author

Nagao, Masaki, primary, Hatae, Akinobu, additional, Mine, Kazuma, additional, Tsutsumi, Soichiro, additional, Omori, Hiroya, additional, Hirata, Marika, additional, Arimatsu, Maaya, additional, Taniguchi, Chise, additional, Watanabe, Takuya, additional, Kubota, Kaori, additional, Katsurabayashi, Shutaro, additional, and Iwasaki, Katsunori, additional

Published
2023
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3. Astrocytic GPCR-Induced Ca 2+ Signaling Is Not Causally Related to Local Cerebral Blood Flow Changes.

Author

Ozawa, Katsuya, Nagao, Masaki, Konno, Ayumu, Iwai, Youichi, Vittani, Marta, Kusk, Peter, Mishima, Tsuneko, Hirai, Hirokazu, Nedergaard, Maiken, and Hirase, Hajime

Subjects
*CALCIUM ions, *CEREBRAL circulation, *SOMATOSENSORY cortex, *G protein coupled receptors, *BLOOD plasma, *BLOOD vessels, *TRANSGENIC mice
Abstract

Activation of Gq-type G protein-coupled receptors (GPCRs) gives rise to large cytosolic Ca2+ elevations in astrocytes. Previous in vitro and in vivo studies have indicated that astrocytic Ca2+ elevations are closely associated with diameter changes in the nearby blood vessels, which astrocytes enwrap with their endfeet. However, the causal relationship between astrocytic Ca2+ elevations and blood vessel diameter changes has been questioned, as mice with diminished astrocytic Ca2+ signaling show normal sensory hyperemia. We addressed this controversy by imaging cortical vasculature while optogenetically elevating astrocyte Ca2+ in a novel transgenic mouse line, expressing Opto-Gq-type GPCR Optoα1AR (Astro-Optoα1AR) in astrocytes. Blue light illumination on the surface of the somatosensory cortex induced Ca2+ elevations in cortical astrocytes and their endfeet in mice under anesthesia. Blood vessel diameter did not change significantly with Optoα1AR-induced Ca2+ elevations in astrocytes, while it was increased by forelimb stimulation. Next, we labeled blood plasma with red fluorescence using AAV8-P3-Alb-mScarlet in Astro-Optoα1AR mice. We were able to identify arterioles that display diameter changes in superficial areas of the somatosensory cortex through the thinned skull. Photo-stimulation of astrocytes in the cortical area did not result in noticeable changes in the arteriole diameters compared with their background strain C57BL/6. Together, compelling evidence for astrocytic Gq pathway-induced vasodiameter changes was not observed. Our results support the notion that short-term (<10 s) hyperemia is not mediated by GPCR-induced astrocytic Ca2+ signaling. [ABSTRACT FROM AUTHOR]

Published
2023
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14. Effect of Yokukansan and Yokukansankachimpihange on Aggressive Behavior, 5-HT Receptors and Arginine Vasopressin Expression in Social Isolation-Reared Mice

Author

Iba, Hikari, primary, Watanabe, Takuya, additional, Matsuzawa, Kanae, additional, Saimiya, Maki, additional, Tanaka, Masako, additional, Nagao, Masaki, additional, Moriyama, Hiroshi, additional, Kubota, Kaori, additional, Katsurabayashi, Shutaro, additional, and Iwasaki, Katsunori, additional

Published
2019
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16. Effect of Lactobacillus paracasei A221‐fermented ginseng on impaired spatial memory in a rat model with cerebral ischemia and β‐amyloid injection

Author

Nagao, Masaki, primary, Yamano, Satoshi, additional, Imagawa, Naoki, additional, Igami, Kentaro, additional, Miyazaki, Toshitsugu, additional, Ito, Hisatomi, additional, Watanabe, Takuya, additional, Kubota, Kaori, additional, Katsurabayashi, Shutaro, additional, and Iwasaki, Katsunori, additional

Published
2019
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18. The Effects of Sansoninto on the Insomnia in Socially Isolated Mice

Author

Kira, Kentaro, Takasaki, Kotaro, Nagao, Masaki, Moriyama, Hiroshi, Ogata, Ayaka, Morikawa, Hanae, Hyakutake, Yuka, Ando, Natsuki, Nishiyama, Yuko, Kubota, Kaori, Katurabayashi, Shutaro, Mishima, Kenichi, Uchida, Naoki, Nishimura, Ryoji, and Iwasaki, Katsunori

Subjects
Mice, Insomnia, Herbal Medicine, Isolated stress, Sansoninto
Published
2015

20. The Japanese Angelica acutiloba root and yokukansan increase hippocampal acetylcholine level, prevent apoptosis and improve memory in a rat model of repeated cerebral ischemia

Author

Nogami-Hara, Ai, primary, Nagao, Masaki, additional, Takasaki, Kotaro, additional, Egashira, Nobuaki, additional, Fujikawa, Risako, additional, Kubota, Kaori, additional, Watanabe, Takuya, additional, Katsurabayashi, Shutaro, additional, Hatip, Funda Bolukbasi, additional, Hatip-Al-Khatib, Izzettin, additional, and Iwasaki, Katsunori, additional

Published
2018
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32. (Digital Presentation) Epitaxially Grown of SiGe on Ge Microbridge and Observation of Strong Resonant Light Emission

Author

Inoue, Takahiro, Wagatsuma, Youya, Ikegaya, Reo, Odashima, Ayaka, Nagao, Masaki, and Sawano, Kentarou

Abstract

We observe strong room-temperature photoluminescence from Ge microbridges formed on Ge-on-Si (110). The Si (110) substrate is employed to fabricate the bridge along [111] direction as uniaxial strain in the [111] direction is expected to be the most effective to bring direct transition. In this study, we grow Ge-on-Si with (110) orientation and fabricate MB along the [111] directions. Due to the low etching rate of the (111) plane, however, etching of the Si under the square-shaped pads is quite difficult. By contrast, we fabricate branch-like MB, where the underneath Si was fully etched owing to the various directions of the etching. As a result, we obtained very strong resonant light emission.

Published
2022
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33. Joint PAJ/JAMA Project - Development of a JASO Gasoline Bench Engine Test for Measuring CCDs

Author

Nagao, Masaki, primary, Omata, Tatsuo, additional, Morinaga, Masataka, additional, Baba, Yukio, additional, Uchiyama, Tsutomu, additional, Kagami, Masayuki, additional, Takarabe, Akira, additional, Okazaki, Junko, additional, Fujimori, Yoshitomo, additional, Komori, Mikiyasu, additional, Shimoyama, Junya, additional, Fujiwara, Satoshi, additional, Hosono, Kyoji, additional, Takei, Yasunori, additional, and Matsudaira, Jun-ichi, additional

Published
1997
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37. Possible involvement of NADPH‐cytochrome P450 reductase and cytochrome b5on β‐ketostearoyl‐CoA reduction in microsomal fatty acid chain elongation supported by NADPH

Author

Nagao, Masaki, Ishibashi, Teruo, Okayasu, Takako, and Imai, Yoh

Abstract

The chain elongation products from [2‐14C]malonyl‐CoA and palmitoyl‐CoA by rat liver microsomes initiated by NADPH were found mainly to be 18:1 and 18:0. When anti‐NADPH‐cytochrome P450 reductase or anti‐cytochrome b5immunoglobulin G were included in this system, not only was the overall chain elongation significantly suppressed, but also a new radioactive product, which was identified as 2‐heptadecanone derived from an intermediate β‐ketostearate, is accumulated depending upon the amounts of IgG added. These results suggest that NADPH‐cytochrome P450 reductase and cytochrome b5participate in the conversion from β‐ketostearoyl‐CoA to β‐hydroxystearoyl‐CoA, which is the first reductive step of the microsomal chain elongating system.

Published
1983
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38. Characterization of Prussian Blue Analogs of the Type M3[Fe(CN)5CO]2·xH2O

Author

Inoue, Hidenari, Kawai, Takashi, Nagao, Masaki, and Yanagisawa, Saburo

Abstract

A series of compounds of the type M3[Fe(CN)5CO]2·xH2O (M=Mn, Co, Ni, Cu, and Zn) have been studied by means of the X-ray diffraction powder patterns, magnetic measurements, infrared, electronic, and M\ddotossbauer spectra. Three compounds M3[Fe(CN)5CO]2·xH2O (M=Mn, Co, and Ni) crystallize in the face-centered cubic system. Copper compound Cu3[Fe(CN)5CO]2·18.5H2O belongs to the tetragonal system, zinc compound Zn3[Fe(CN)5CO]2·13.5H2O being amorphous. The structure of these compounds is described as a three-dimensional framework of two octahedral coordination units FeC6in the low spin state and MN10⁄3O8⁄3in the high spin state. The back donation in the Fe(CN)5CO3−moiety is enhanced by the bridge formation through CN−and CO ligands, i.e.upon the formation of M3[Fe(CN)5CO]2·xH2O from the corresponding sodium salt.

Published
1982
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44. Virally induced CRISPR/Cas9-based knock-in of fluorescent albumin allows long-term visualization of cerebral circulation in infant and adult mice.

Author

Vittani M, Knak PAG, Fukuda M, Nagao M, Wang X, Kjaerby C, Konno A, Hirai H, Nedergaard M, and Hirase H

Abstract

Albumin, a protein produced by liver hepatocytes, represents the most abundant protein in blood plasma. We have previously engineered a liver-targeting adeno-associated viral vector (AAV) that expresses fluorescent protein-tagged albumin to visualize blood plasma in mice. While this approach is versatile for imaging in adult mice, transgene expression vanishes when AAV is administered in neonates due to dilution of the episomal AAV genome in the rapidly growing liver. Here, we use CRISPR/Cas9 genome editing to insert the fluorescent protein mNeonGreen (mNG) gene into the albumin (Alb) locus of hepatocytes to produce fluorescently labeled albumin (Alb-mNG). We constructed a CRISPR AAV that includes ∼1 kb homologous arms around Alb exon 14 to express Alb-mNG. Subcutaneous injection of this AAV with AAV-CMV-Cas9 in postnatal day 3 mice resulted in two-photon visualization of the cerebral cortex vasculature within ten days. The expression levels of Alb-mNG were persistent for at least three months and were so robust that vasomotion and capillary blood flow could be assessed transcranially in early postnatal mice. This knock-in approach provides powerful means for micro- and macroscopic imaging of cerebral vascular dynamics in postnatal and adult mice.

Published
2023
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