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27 results on '"Nahrendorf, Wiebke"'

4. A Behavior-Based Circuit Model of How Outcome Expectations Organize Learned Behavior in Larval 'Drosophila'

Author

Schleyer, Michael, Saumweber, Timo, and Nahrendorf, Wiebke

Abstract

Drosophila larvae combine a numerically simple brain, a correspondingly moderate behavioral complexity, and the availability of a rich toolbox for transgenic manipulation. This makes them attractive as a study case when trying to achieve a circuit-level understanding of behavior organization. From a series of behavioral experiments, we suggest a circuitry of chemosensory processing, odor-tastant memory trace formation, and the "decision" process to behaviorally express these memory traces--or not. The model incorporates statements about the neuronal organization of innate vs. conditioned chemosensory behavior, and the types of interaction between olfactory and gustatory pathways during the establishment as well as the behavioral expression of odor-tastant memory traces. It in particular suggests that innate olfactory behavior is responsive in nature, whereas conditioned olfactory behavior is captured better when seen as an action in pursuit of its outcome. It incorporates the available neuroanatomical and behavioral data and thus should be useful as scaffold for the ongoing investigations of the chemo-behavioral system in larval "Drosophila".

Published
2011
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5. Repeat controlled human malaria infection of healthy UK adults with blood-stage Plasmodium falciparum: Safety and parasite growth dynamics

Author

Salkeld, Jo, primary, Themistocleous, Yrene, additional, Barrett, Jordan R., additional, Mitton, Celia H., additional, Rawlinson, Thomas A., additional, Payne, Ruth O., additional, Hou, Mimi M., additional, Khozoee, Baktash, additional, Edwards, Nick J., additional, Nielsen, Carolyn M., additional, Sandoval, Diana Muñoz, additional, Bach, Florian A., additional, Nahrendorf, Wiebke, additional, Ramon, Raquel Lopez, additional, Baker, Megan, additional, Ramos-Lopez, Fernando, additional, Folegatti, Pedro M., additional, Quinkert, Doris, additional, Ellis, Katherine J., additional, Poulton, Ian D., additional, Lawrie, Alison M., additional, Cho, Jee-Sun, additional, Nugent, Fay L., additional, Spence, Philip J., additional, Silk, Sarah E., additional, Draper, Simon J., additional, and Minassian, Angela M., additional

Published
2022
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6. Protection against malaria after immunization by chloroquine prophylaxis and sporozoites is mediated by preerythrocytic immunity

Author

Bijker, Else M., Bastiaens, Guido J. H., Teirlinck, Anne C., van Gemert, Geert-Jan, Graumans, Wouter, van de Vegte-Bolmer, Marga, Siebelink-Stoter, Rianne, Arens, Theo, Teelen, Karina, Nahrendorf, Wiebke, Remarque, Edmond J., Roeffen, Will, Jansens, Annemieke, Zimmerman, Dunja, Vos, Martijn, van Schaijk, Ben C. L., Wiersma, Jorien, van der Ven, André J. A. M., de Mast, Quirijn, van Lieshout, Lisette, Verweij, Jaco J., Hermsen, Cornelus C., Scholzen, Anja, and Sauerwein, Robert W.

Published
2013

7. Repeat controlled human malaria infection of healthy UK adults with blood-stagePlasmodium falciparum: safety and parasite growth dynamics

Author

Salkeld, Jo, primary, Themistocleous, Yrene, additional, Barrett, Jordan R., additional, Mitton, Celia H., additional, Rawlinson, Thomas A., additional, Payne, Ruth O., additional, Hou, Mimi M., additional, Khozoee, Baktash, additional, Edwards, Nick J., additional, Nielsen, Carolyn M., additional, Sandoval, Diana Muñoz, additional, Bach, Florian A., additional, Nahrendorf, Wiebke, additional, Ramon, Raquel Lopez, additional, Baker, Megan, additional, Ramos-Lopez, Fernando, additional, Folegatti, Pedro M., additional, Quinkert, Doris, additional, Ellis, Katherine J., additional, Poulton, Ian D., additional, Lawrie, Alison M., additional, Cho, Jee-Sun, additional, Nugent, Fay L., additional, Spence, Philip J., additional, Silk, Sarah E., additional, Draper, Simon J., additional, and Minassian, Angela M., additional

Published
2022
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9. Adaptive T cells regulate disease tolerance in human malaria

Author

Sandoval, Diana Muñoz, primary, Bach, Florian, additional, Nahrendorf, Wiebke, additional, Ivens, Alasdair, additional, Mazurczyk, Michalina, additional, Themistocleous, Yrene, additional, Silk, Sarah E., additional, Barrett, Jordan R., additional, Edwards, Nick J., additional, Napolitani, Giorgio, additional, Minassian, Angela M., additional, Draper, Simon J., additional, and Spence, Philip J., additional

Published
2021
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11. Mapping immune variation and var gene switching in naive hosts infected with Plasmodium falciparum

Author

Milne, Kathryn, primary, Ivens, Alasdair, additional, Reid, Adam J, additional, Lotkowska, Magda E, additional, O'Toole, Aine, additional, Sankaranarayanan, Geetha, additional, Munoz Sandoval, Diana, additional, Nahrendorf, Wiebke, additional, Regnault, Clement, additional, Edwards, Nick J, additional, Silk, Sarah E, additional, Payne, Ruth O, additional, Minassian, Angela M, additional, Venkatraman, Navin, additional, Sanders, Mandy J, additional, Hill, Adrian VS, additional, Barrett, Michael, additional, Berriman, Matthew, additional, Draper, Simon J, additional, Rowe, J Alexandra, additional, and Spence, Philip J, additional

Published
2021
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13. Author response: Mapping immune variation and var gene switching in naive hosts infected with Plasmodium falciparum

Author

Milne, Kathryn, primary, Ivens, Alasdair, additional, Reid, Adam J, additional, Lotkowska, Magda E, additional, O'Toole, Aine, additional, Sankaranarayanan, Geetha, additional, Munoz Sandoval, Diana, additional, Nahrendorf, Wiebke, additional, Regnault, Clement, additional, Edwards, Nick J, additional, Silk, Sarah E, additional, Payne, Ruth O, additional, Minassian, Angela M, additional, Venkatraman, Navin, additional, Sanders, Mandy J, additional, Hill, Adrian VS, additional, Barrett, Michael, additional, Berriman, Matthew, additional, Draper, Simon J, additional, Rowe, J Alexandra, additional, and Spence, Philip J, additional

Published
2021
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17. Diverse outcomes of controlled human malaria infection originate from host-intrinsic immune variation and not var gene switching

Author

Milne, Kathryn, primary, Ivens, Alasdair, additional, Reid, Adam J., additional, Lotkowska, Magda E., additional, O’Toole, Áine, additional, Sankaranarayanan, Geetha, additional, Sandoval, Diana Muñoz, additional, Nahrendorf, Wiebke, additional, Regnault, Clement, additional, Edwards, Nick J., additional, Silk, Sarah E., additional, Payne, Ruth O., additional, Minassian, Angela M., additional, Venkatraman, Navin, additional, Sanders, Mandy, additional, Hill, Adrian V.S., additional, Barrett, Michael P., additional, Berriman, Matthew, additional, Draper, Simon J., additional, Rowe, J. Alexandra, additional, and Spence, Philip J., additional

Published
2020
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19. An enhanced toolkit for the generation of knockout and marker-free fluorescent Plasmodium chabaudi

Author

Marr, Edward J, primary, Milne, Rachel M, additional, Anar, Burcu, additional, Girling, Gareth, additional, Schwach, Frank, additional, Mooney, Jason P, additional, Nahrendorf, Wiebke, additional, Spence, Philip J, additional, Cunningham, Deirdre, additional, Baker, David A, additional, Langhorne, Jean, additional, Rayner, Julian C, additional, Billker, Oliver, additional, Bushell, Ellen S, additional, and Thompson, Joanne, additional

Published
2020
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20. An enhanced toolkit for the generation of knockout and marker-free fluorescent Plasmodium chabaudi

Author

Marr, Edward J, Milne, Rachel M, Anar, Burcu, Girling, Gareth, Schwach, Frank, Mooney, Jason P, Nahrendorf, Wiebke, Spence, Philip J, Cunningham, Deirdre, Baker, David A, Langhorne, Jean, Rayner, Julian C, Billker, Oliver, Bushell, Ellen S., Thompson, Joanne, Marr, Edward J, Milne, Rachel M, Anar, Burcu, Girling, Gareth, Schwach, Frank, Mooney, Jason P, Nahrendorf, Wiebke, Spence, Philip J, Cunningham, Deirdre, Baker, David A, Langhorne, Jean, Rayner, Julian C, Billker, Oliver, Bushell, Ellen S., and Thompson, Joanne

Abstract

The rodent parasite Plasmodium chabaudi is an important in vivo model of malaria. The ability to produce chronic infections makes it particularly useful for investigating the development of anti- Plasmodium immunity, as well as features associated with parasite virulence during both the acute and chronic phases of infection. P. chabaudi also undergoes asexual maturation (schizogony) and erythrocyte invasion in culture, so offers an experimentally-amenable in vivo to in vitro model for studying gene function and drug activity during parasite replication. To extend the usefulness of this model, we have further optimised transfection protocols and plasmids for P. chabaudi and generated stable, fluorescent lines that are free from drug-selectable marker genes. These mother-lines show the same infection dynamics as wild-type parasites throughout the lifecycle in mice and mosquitoes; furthermore, their virulence can be increased by serial blood passage and reset by mosquito transmission. We have also adapted the large-insert, linear PlasmoGEM vectors that have revolutionised the scale of experimental genetics in another rodent malaria parasite and used these to generate barcoded P. chabaudi gene-deletion and -tagging vectors for transfection in our fluorescent P. chabaudi mother-lines. This produces a tool-kit of P. chabaudi lines, vectors and transfection approaches that will be of broad utility to the research community.

Published
2020
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21. Mosquito transmission of the rodent malaria parasite Plasmodium chabaudi

Author

Spence Philip J, Jarra William, Lévy Prisca, Nahrendorf Wiebke, and Langhorne Jean

Subjects
Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Serial blood passage of Plasmodium increases virulence, whilst mosquito transmission inherently regulates parasite virulence within the mammalian host. It is, therefore, imperative that all aspects of experimental malaria research are studied in the context of the complete Plasmodium life cycle. Methods Plasmodium chabaudi chabaudi displays many characteristics associated with human Plasmodium infection of natural mosquito vectors and the mammalian host, and thus provides a unique opportunity to study the pathogenesis of malaria in a single infection setting. An optimized protocol that permits efficient and reproducible vector transmission of P. c. chabaudi via Anopheles stephensi was developed. Results and conclusions This protocol was utilized for mosquito transmission of genetically distinct P. c. chabaudi isolates, highlighting differential parasite virulence within the mosquito vector and the spectrum of host susceptibility to infection initiated via the natural route, mosquito bite. An apposite experimental system in which to delineate the pathogenesis of malaria is described in detail.

Published
2012
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26. Cross-stage immunity for malaria vaccine development

Author

Nahrendorf, Wiebke, Scholzen, Anja, Sauerwein, Robert W, and Langhorne, Jean

Subjects
Model organisms, FOS: Clinical medicine, parasitic diseases, Immunology, Infectious Disease, 3. Good health
Abstract

A vaccine against malaria is urgently needed for control and eventual eradication. Different approaches are pursued to induce either sterile immunity directed against pre-erythrocytic parasites or to mimic naturally acquired immunity by controlling blood-stage parasite densities and disease severity. Pre-erythrocytic and blood-stage malaria vaccines are often seen as opposing tactics, but it is likely that they have to be combined into a multi-stage malaria vaccine to be optimally safe and effective. Since many antigenic targets are shared between liver- and blood-stage parasites, malaria vaccines have the potential to elicit cross-stage protection with immune mechanisms against both stages complementing and enhancing each other. Here we discuss evidence from pre-erythrocytic and blood-stage subunit and whole parasite vaccination approaches that show that protection against malaria is not necessarily stage-specific. Parasites arresting at late liver-stages especially, can induce powerful blood-stage immunity, and similarly exposure to blood-stage parasites can afford pre-erythrocytic immunity. The incorporation of a blood-stage component into a multi-stage malaria vaccine would hence not only combat breakthrough infections in the blood should the pre-erythrocytic component fail to induce sterile protection, but would also actively enhance the pre-erythrocytic potency of this vaccine. We therefore advocate that future studies should concentrate on the identification of cross-stage protective malaria antigens, which can empower multi-stage malaria vaccine development.

27. An enhanced toolkit for the generation of knockout and marker-free fluorescent Plasmodium chabaudi

Author

Edward J. Marr, Burcu Anar, David A. Baker, Ellen Bushell, Jason P. Mooney, Philip J Spence, Oliver Billker, Wiebke Nahrendorf, Joanne Thompson, Rachel M Milne, Julian C. Rayner, Frank Schwach, Deirdre Cunningham, Jean Langhorne, Gareth Girling, Mooney, Jason P [0000-0002-2824-124X], Nahrendorf, Wiebke [0000-0002-4503-8761], Langhorne, Jean [0000-0002-2257-9733], Billker, Oliver [0000-0003-1716-168X], Thompson, Joanne [0000-0002-3343-2278], and Apollo - University of Cambridge Repository

Subjects
030231 tropical medicine, malaria, Virulence, Medicine (miscellaneous), Transfection, Plasmodium, General Biochemistry, Genetics and Molecular Biology, Schizogony, Plasmodium chabaudi, 03 medical and health sciences, Plasmid, 0302 clinical medicine, PlasmoGem, parasitic diseases, Parasite hosting, Gene, 030304 developmental biology, 0303 health sciences, biology, Articles, Method Article, biology.organism_classification, Cell biology, 3. Good health, Malaria
Abstract

The rodent parasite Plasmodium chabaudi is an important in vivo model of malaria. The ability to produce chronic infections makes it particularly useful for investigating the development of anti-Plasmodium immunity, as well as features associated with parasite virulence during both the acute and chronic phases of infection. P. chabaudi also undergoes asexual maturation (schizogony) and erythrocyte invasion in culture, so offers an experimentally-amenable in vivo to in vitro model for studying gene function and drug activity during parasite replication. To extend the usefulness of this model, we have further optimised transfection protocols and plasmids for P. chabaudi and generated stable, fluorescent lines that are free from drug-selectable marker genes. These mother-lines show the same infection dynamics as wild-type parasites throughout the lifecycle in mice and mosquitoes; furthermore, their virulence can be increased by serial blood passage and reset by mosquito transmission. We have also adapted the large-insert, linear PlasmoGEM vectors that have revolutionised the scale of experimental genetics in another rodent malaria parasite and used these to generate barcoded P. chabaudi gene-deletion and –tagging vectors for transfection in our fluorescent P. chabaudi mother-lines. This produces a tool-kit of P. chabaudi lines, vectors and transfection approaches that will be of broad utility to the research community.

Published
2020
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