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The present article describes some characteristics of the effect of essential oil (EO) extracted from Ziziphora clinopodioides harvested from Armenian highlands on microglial cell lines (BV-2 wild-type (WT) and acyl-CoA oxidase1 (ACOX1)-deficient (Acox1–/–) cells). The mutant cell line was used as a model to investigate cellular oxidative damage following EO treatment. The main components of the tested EO were pulegone, isomenthone, 1,8-cineole, piperitone, and neomenthole, with concentrations of 42.1%, 9.7%, 8.22%, 7.35%, and 5.9%, respectively, in plants harvested from the high-altitude Armenian landscape. The IC50 value of the EO in the DPPH assay was 7.025 uL/mL. The sub-cytotoxic concentrations (based on the MTT assay) for both cell lines were 5 × 10–1 uL/mL. The catalase activity of the WT cells was decreased following 24-h treatment with the EO, but that of Acox1–/– BV-2 cellswas increased. ACOX1 activity was decreased (up to 49%) at 72hof treatment. These results show the protective effect of the tested EO on Acox1–/– mutantcells.

Compounds of plant origin are considered promising alternative approaches in the development of medicines for the prevention and treatment of cancer. The large diversity of herbal species still requires careful exploration as a source for new anticancer compounds. The goal of the study was to screen different herbal extracts traditionally used in Armenian folk medicine for their cytotoxic effect against some cancer cell lines, and to find the prospective plant species among them. The cytotoxicity of the plant ethanol extracts was evaluated with MTT test against HeLa (human cervical carcinoma) and A549 (human lung adenocarcinoma) cells. Antioxidant properties were assessed with DPPH free radical scavenging assay. Five of the tested ten herbal extracts exhibited significant growth-inhibiting activity on HeLa cells. Moreover, Alchemilla smirnovii and Hypericum alpestre extracts also showed potent cytotoxicity on human lung adenocarcinoma cells. These two plants possessed high antiradical activity as well. Their DPPH stoichiometric values were 0.4234 and 0.14437 respectively, meaning that 1 µg of plant extract brought the reduction of DPPH equal to the respective stoichiometric values in µg. Thus, A. smirnovii and H. alpestre extracts expressed themselves as potent cytotoxic and antioxidant agents and could have promising anticancer potential. Further evaluation of their in vivo anticancer properties has much interest.

Adverse effect of oxidative stress is a huge problem in medicine. In several circumstances, exogenous antioxidants are needed to regulate the amount of reactive species in the body. Plants are considered as a promising source for new antioxidant compounds. The goal of this study was to evaluate the antioxidant potential of extracts of the following herbs: Agrimonia eupatoria, Hypericum alpestre, Rumex obtusifolius and Sanguisorba officinalis using different chemical-based tests. GC-MS technique was used for identification of plant’s volatile bio-active constituents. The results revealed good potential of the tested herbs as sources for new antioxidant compounds.

Polyphenols are a group of plant secondary metabolites characterized with the presence of multiples of phenol structural units. Tannins are plant-originated phenolic substances, which play an important role in plant defense system as well as have found applications in medicine, industry, and other fields of human activity. Despite these, the mechanisms of bioavailability of these substances are poorly studied and even there are very few amounts of sources gathering recent studies concerning this issue. So the main purpose of this review is to focus on the known peculiarities and possible ways of the bioavailability of these oligomeric substances. Due to the ability of tannins to bind proteins and other macromolecules, they are responsible for astringency or bitterness of red wines and also for the anti-nutritional effects, which can be reduced by the action of tannase. In nutriceutical industry, tannins are used as natural preservers and antioxidants. They have also a wide range of medicinal application as anti-hemorrhagic and hemostatic, anti-malarial and antitumor, antimicrobial, antioxidant, anti-tyrosinase, anthelmintic, antiviral, and anti-inflammatory activities among others. It is suggested that absorption of tannins is correlated with their polymerization degree and the activity of these components depends on their absorption in the gut, metabolism and, therefore, the bioavailability. Despite numerous promising applications, there are still plenty of open spaces to fully understand determinants of the bioavailability of tannins and other oligomeric polyphenols pivotal for the expression of their biological activity.

Aims: This work aimed to reveal some mechanisms of influence of three basil EO on the microglial cells, as recently, research data stated that these oils have anti-aging and neuroprotective properties, and they are found to be effective against some forms of neurodegeneration. Background: The microglial cells play a pivotal role as the neuroprotective agents against neuroinflammation. Ocimum subspecies are a rich source of essential oils (EO) and used to be applied since antiquity for different purposes, including the prevention and treatment of various diseases. Objective: In this study, the influence of the essential oils extracted from three basil cultivars (O. basilicum var. purpureum, O. basilicum var. thyrsiflora, and O. x citriodorum), possessing remarkable antioxidant activity, on the activity of the main antioxidant enzymes in microglial BV-2 wild type (WT) and Acetyl-CoA oxidase deficient cell lines (Acox1-/-) was evaluated. Method: All manipulations were carried out using murine microglial BV-2 cell lines (BV-2, Acyl- CoA oxidase type 1 (ACOX1) deficient mutants (Acox1-/-), and WT cells). Result: Data included in the present article state that plant origin substances can play a role in the regulation of enzymatic antioxidant activity of cells. EOs extracted from the Ocimum different cultivars are able to trigger the activity of acetyl-CoA oxidase type 1 (or palmitoyl-CoA oxidase type 1), which can serve as a basis for the regulation of redox deviation in WT cells. Conclusion: Thus, it can be suggested to apply them for the prevention of some processes, which can influence aging, as the process of ageing is commonly associated with mitochondrial dysfunction, oxidative stress caused by the increased level of free radical production, dysfunction of the microglia, high blood pressure, and so on. Practical Applications: The microglial cells play a pivotal role as the neuroprotective agents against neuroinflammation. Different data included in the present article described that plant origin substances can play a role in regulating the enzymatic antioxidant activity of cells. EOs extracted from different cultivars of Ocimum are able to trigger the activity of acetyl-CoA oxidase type 1 (palmitoyl-CoA oxidase type 1), which can serve as a basis for the regulation of redox deviation in WT cells. Therefore, it can be proposed to apply them as prevention of some processes, which can influence aging, since the process of aging is commonly associated with mitochondrial dysfunction, oxidative stress caused by the increased level of free radical production, dysfunction of the microglia, high blood pressure and so on.

Essential oils are of great interest for their analgesic and anti-inflammatory properties. We aimed to study the content of the essential oil of the Origanum vulgare of the Armenian highlands (OVA) in different periods of vegetation and to investigate its antinociceptive and anti-inflammatory effects in mice (in vivo) and cytotoxic action in cultured cells (in vitro). OVA essential oil was extracted from fresh plant material by hydro-distillation.For OVA essential oil contents determination the gas chromatography-mass spectrometry method was used. Formalin and hot plate tests and analysis of cell viability using the methyl-thiazolyl-tetrazolium (MTT) assay were used.The maximal content of β-caryophyllene and β-caryophyllene oxide in OVA essential oil was revealed in the period of blossoming (8.18% and 13.36%, correspondently). In the formalin test, 4% OVA essential oil solution (3.5 mg/mouse) exerts significant antinociceptive and anti-inflammatory effects (P = 0.003). MTT assay shows approximately 60% cytotoxicity in HeLa and Vero cells for 2.0 μL/mL OVA essential oil in media.The wild oregano herb of Armenian highlands, harvested in the blossoming period, may be considered as a valuable source for developing pain-relieving preparations.

Background: Origanum vulgare is used in Armenian cuisine as well as in folk medicine. The aim of this investigation was to determine the peculiarities of the chemical composition of Armenian flora’s oregano essential oil (EO) and ethanol extract (EE) and to characterize mainly antioxidant and antibacterial properties in vitro. Methods: EO of plant aerial parts was extracted by the hydro-distillation method, and the composition was analyzed by gas-chromatography (GC). EE was prepared using ethanol as a solvent. Antioxidant, antibacterial and other biological activity of EO and EE was determined using appropriate methods. Results: The terpenes were found to be the greatest part of oregano EO (β-caryophyllene epoxide - 13.3 %; β- caryophyllene -8.2 %; ο-cymene - 5.2 %). The flavonoid content of EE was 3.9±0.7 mg g-1 catechin equivalents. Antiradical activities of EO and EE expressed with half-maximum inhibitory concentrations were 1057 µg mL-1 and 19.97 µg mL-1. The antioxidant index in the case of using extract (1000 µg mL-1) was 77.3±1.5 %. The metal chelating activity of EE was 74.5±0.2 %. The tyrosinase inhibitory activities of EO, EE and arbutin were 26.5±0.3 %, 6.5±0.2 % and 50±0.1 %, respectively. Both EO and EE of oregano were able to suppress the growth of testmicroorganisms including clinical pathogen isolates, but have moderate antimicrobial activity. EO antibacterial activity against ampicillin-resistant Escherichia coli was similar to the activity against non-resistant strain. EE was not active against ampicillin-resistant E. coli in contrast to non-resistant one. Conclusion: The results indicate high antioxidant and moderate antibacterial as well as high antiradical, metal chelating, tyrosinase inhibitory activity of oregano EO and EE, which can be used as a natural source of terpenes, flavonoids and other phytochemicals in medicine, pharmaceutics, cosmetics and food industry. EO has antibacterial activity also against ampicillin-resistant E. coli.

Overcoming the antibiotic resistance is nowadays a challenge. There is still no clear strategy to combat this problem. Therefore, the urgent need to find new sources of antibacterial agents exists. According to some literature, substances of plant origin are able to overcome bacterial resistance against antibiotics. Alkanna species plants are among the valuable producers of these metabolites. But there is a problem of obtaining the standardized product. So, this review is focused on the discussion of the possibilities of biotechnological production of antimicrobial agents from Alkanna genus species against some microorganisms including antibiotic resistant bacterial strains.

Investigation of dietary biologically active phytochemicals is of interest due to the availability, low cost, and low rate of side effects of these substances. The main objective of this work was to investigate the influence of the essential oil (EO) extracted from the aerial parts of Artemisia dracunculus on the antioxidant capacity of cells as this plant is one of the most available and widely used as spice and in folk medicine. For this, BV-2 microglial wild type (WT) and acyl-CoA oxidase type 1 (ACOX1) deficient cells (Acox1-/- ) were used. Acox1-/- cells were applied as the model of cellular oxidative damage. The main component of EO of A. dracunculus was estragole, which was reaching 84.9% in plants cultivated at high altitude Armenian landscape. IC50 value of EO in 1,1-diphenyl-2-picrylhydrazyl assay was 94.2 µg/ml. Sub-cytotoxic concentration in the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test for both BV-2 WT and Acox1-/- cell lines was 5.10-1 µg/ml. Seventy-two-hours treatment with EO leads to the increased viability (up to 12% in WT and up to 14% -in BV-2 Acox1-/- cells). The 48-hr treatment increased the ACOX1 activity up to 70% in WT cells. Catalase and superoxide dismutase activities of both cell lines increased following the 24-48-hr treatment. These results indicate that A. dracunculus EO can be considered as a potential protective agent useful in preventive medicine.

Plant extracts serve as reducing and coating agents and are, therefore, commonly employed for the generation of silver (Ag) nanoparticles (NPs). Plant extract mediated synthesis of Ag NPs is a green, environmentally friendly and cost-effective technique which offers a new and potential alternative to chemically synthesized NPs, decreasing the utilization of hazardous and toxic chemicals and protecting the environment. Origanum vulgare L. extracts were evaluated for total flavonoid and phenol content. The free radical scavenging activity was determined employing 2,2-diphenyl-1-picrylhydrazyl assay. Ag NPs were produced exploiting ethanolic extracts of O. vulgare L. leaves. The generation of Ag NPs was carried out both in light and dark conditions. The biosynthesized Ag NPs were characterized employing microscopic and spectroscopic techniques. Antibacterial activities of Ag NPs were determined following appropriate methods. The results revealed that energy of photons was required to reduce Ag+ to Ag0. According to scanning electron microscopy reports, biologically formed Ag NPs ranged in size from 1 to 50 nmand were presented instability causing aggregation. They indicated that O. vulgare L. extracts were rich in flavonoids and phenols and exhibited strong antioxidant activity. Ag NPs exhibited good antibacterial activity immediately after production. Gram-positive strains showed higher sensitivity to Ag NPs compared to Gram-negative stains. Ag NPs can serve as an effective antibacterial agent against antibiotic-resistant strains. The kanamycin-resistant strain was more sensitive to Ag NPs than the ampicillin-resistant strain. Thus, Origanum extract-mediated synthesized Ag NPs can be recommended as alternative effective antibacterial agents, but their activity depended on bacterial species and strains.

The collapse of the old order in the Russian Empire in 1917 gave an opportunity to intellectuals on imperial borders to draft and implement their own visions of the future. The language debate in Dagestan was crucial in these plans given the multi-national character of the country. While Muslim scholars agreed on the importance of language, their opinions differed in the choice of Arabic, Turkic, various Dagestani languages or Russian. On the basis of Muslim periodicals, historical tracts of the period and official reports of political meetings, this paper examines the positions of Dagestaniʿulamāʾon the political significance of language. I argue that at a time of revolution different concepts of the state demanded a certain language profile at schools. There is a direct link between the choice of a political project and a preferred language of instruction.

The history of the Caucasus is replete with numerous scenarios of hegemonic struggles between empires and repeated rebellions among the region’s indigenous nations. In the context of resistance aga...

Cellular redox homeostasis is a state of balance between the formation of Usually Reactive Oxygen and / or Nitrogen Species (ROS/RNS), endogenous antioxidant defence systems, and exogenous dietary antioxidants. The disturbance of redox homeostasis, by the overproduction of endogenous ROS/RNS, may increase the risk of development of so-called civilisation diseases. The solution seems to be either the increased production of endogenous or consumption of exogenous antioxidants. Plant-borne antioxidants act via different chemical and molecular mechanisms, such as decreasing the level of oxidative damage in cells directly by reacting with ROS/RNS or indirectly - by inhibition of the activity and expression of free radical generating enzymes or by enhancing the activity or expression of intracellular antioxidant defence enzymes. Despite the fact that the Caucasian flora is rich of health promoting edible/medicinal plants, recent studies concerning the biological activity of these plants are very scarce. This review is summarising the state-of-art on the health-promoting potential of plants representing the Caucasian flora, whose antioxidant capacity have been investigated in various

The diversity of plants in Armenia is due to the singularity of natural environment. However, biochemical activity of these plants has not been studied well. The goal was to investigate biological activities as well as antihyperglycemic properties of Rumex obtusifolius L. in rabbits with hyperglycemia. According to obtained data, R. obtusifolius extracts possessed high antioxidant activity in chemical-based tests. GS/MS analysis of its methanol extracts allowed identification of several compounds, which could have high contribution on biological activities. The sub-cytotoxic concentration of investigated extract was 10 mgmL-1on Murine microglial BV-2 WT cell lines. Oral administration of ethanol extract showed significant effect on hyperglycemia, reducing fasting glucose levels (57.3%, pR. obtusifolius extracts could be source of new biologically active compounds and recommended for treatment of diabetes mellitus.

Herbal medicine requires searching for new sources with antimicrobial activity. Alkanna sp. (Boraginaceae) is widely used in medicine due to detoxification and antibacterial effects. The aim of this study was to obtain Alkanna orientalis (L.) Boiss. plant callus extracts, to investigate antimicrobial activity of extracts against bacteria and yeast and to reveal responsible mechanisms. Callus tissue cultures have been obtained using different nutrient media. Antimicrobial activity and minimal inhibitory concentrations (MIC) were determined by the methods of extract diffusion in agar and dilution using different test-microorganisms. Quantity and quality of naphthoquinones were determined using spectrophotometric and high performance liquid chromatographic analyses. H+/K+ exchange by whole cells was assayed using selective electrodes, ATPase activity and SH-groups number of membrane vesicles—by spectrophotometric methods. It was revealed that callus extracts containing naphthoquinones (0.19 ± 0.01 %) possessed bacteriostatic activity against gram-positive bacteria including pathogenic ones (MIC were 125–750 μg/mL−1), and bactericidal activity against lactic acid bacteria (Lactobacillus acidophilus, L. rhamnosus) (MIC was 250 μg/mL). MIC against Enterococcus hirae was 250 μg/mL of callus extract or 31.25 μg/mL of shikonin. It depended on the medium content, cultivation duration and the activity remained for 10–11 years. Moreover, intact root extracts inhibited H+/K+ exchange of E. hirae but callus extracts had a stronger effect. The inhibitor, N,N′-dicyclohexylcarbodiimide (DCCD)-sensitive H+/K+ exchange was changed; ATPase activity and SH-groups number were lowered by two and more fold under the influence of the extracts. Alkanet root and callus tissues extracts were concluded to have a high antimicrobial activity. So, they might directly affect the FOF1-ATPase which in turn regulates the bacterial growth. These results are useful for further investigation of alkanet extracts composition and their application as an alternative antimicrobial agent in pharmaceutical and food industry as well as in medicine.

Background The plants belonging to the Ocimum genus of the Lamiaceae family are considered to be a rich source of essential oils which have expressed biological activity and use in different area of human activity. There is a great variety of chemotypes within the same basil species. Essential oils from three different cultivars of basil, O. basilicum var. purpureum, O. basilicum var. thyrsiflora, and O. citriodorum Vis. were the subjects of our investigations. Methods The oils were obtained by steam distillation in a Clevenger-type apparatus. The gas chromatography mass selective analysis was used to determine their chemical composition. The antioxidant activities of these essential oils were measured using 1,1-diphenyl-2-picrylhydrazyl assays; the tyrosinase inhibition abilities of the given group of oils were also assessed spectophotometrically, and the antimicrobial activity of the essential oils was determined by the agar diffusion method, minimal inhibitory concentrations were expressed. Results According to the results, the qualitative and quantitative composition of essential oils was quite different: O. basilicum var. purpureum essential oil contained 57.3% methyl-chavicol (estragol); O. basilicum var. thyrsiflora oil had 68.0% linalool. The main constituents of O. citriodorum oil were nerol (23.0%) and citral (20.7%). The highest antioxidant activity was demonstrated by O. basilicum var. thyrsiflora essential oil. This oil has also exhibited the highest tyrosinase inhibition level, whereas the oil from O. citriodorum cultivar demonstrated the highest antimicrobial activity. Conclusions The results obtained indicate that these essential oils have antioxidant, antibacterial and antifungal activity and can be used as natural antioxidant and antimicrobial agents in medicine, food industry and cosmetics.

One of the tasks in contemporary biotechnology, pharmacology and other fields of human activities is to obtain biologically active substances from plants. They are very essential in the treatment of many diseases due to their actually high therapeutic value without visible side effects. However, sometimes the possibility of obtaining the metabolites is limited due to the reduction of wild-growing plants. That is why the plant cell cultures are of great interest as alternative sources of biologically active substances. Besides, during the monitored cultivation, it is possible to obtain substances that are not synthesized by plants in nature. Isolated culture of Ajuga genevensis with high growth activity and ability of regeneration was obtained using MS nutrient medium. The agar-diffusion method showed that aqueous extracts of callus culture revealed high antimicrobial activity towards various gram-positive (Bacillus subtilis A1WT; B. mesentericus WDCM 1873; Staphylococcus aureus WDCM 5233; Staph. citreus WT) and gram-negative (Escherichia coli WKPM M-17; Salmonella typhimurium TA 100) microorganisms. The broth dilution method revealed that the minimal and half maximal inhibitory concentration values against E. coli corresponded to the 70 μg/mL and 140 μg/mL concentration of the extract respectively. According to the photochemiluminescent analysis, callus tissue extracts of leaf and root origin showed higher antioxidant activity than the same quantity of A. genevensis intact plant extract. A. genevensis intact plant and callus culture extracts showed no cytotoxic effect on K-562 suspension cell line of human chronic myeloid leukemia. The GC-MS analysis showed deep differences between the qualitative and quantitative composition of callus culture and intact plant extracts. Hexacosane (11.17%); n-hexadecanoic acid (9.33%); and 2-methoxy-4-vinylphenol (4.28%) were the main components of intact plant extracts. 10-Methylnonadecane (57.0%); methoxyacetic acid, 2-tetradecyl ester (17.75%) and 1-Bromopentadecane (14.55%) were the main components of A. genevensis callus culture extracts. Obtained data indicate that callus culture of A. genevensis can be used as an alternative source of biologically active substances., {"references":["S. Hellwig, J. Drossard, R.M. Twyman, and R. Fischer, \"Plant cell cultures for the production of recombinant proteins,\" Nat Biotech, 2004, vol. 22, pp. 1415-1422.","N. Darbinian-Sarkissian, A. Darbinyan, J. Otte, S. Radhakrishnan, B.E. Sawaya et al., \"p27SJ, a novel protein in St John's Wort, that suppresses expression of HIV-1 genome,\" Gene Therapy. 2006, vol.1, pp.288–295.","M. 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Plant cell redox homeostasis is formed as a result of the balance between the accumulation of reactive oxygen species (ROS), the functioning of the antioxidant enzymes system and antioxidants with low molecular weight. Complex of different changes occurs in plants under stress conditions which often lead to a variety of the intracellular and tissue functional disorders. Under these conditions for the survival, the functioning of the systems of homeostasis maintaining is extremely important. Understanding of the molecular mechanisms of resistance formation to adverse environmental factors is one of the most urgent issues that will help to cope with the problem of increasing plant resistance to stressors. Maintenance of cellular homeostasis in plants under the influence of various external factors is provided by a number of protective systems. Organization of metabolic pathways in plants characterized by having two main separate compartments, generating ATP and reducing equivalents: chloroplasts and mitochondria. The interaction of these two cell energetic organelles with opposite types of functions in plant involves in metabolite fluxes organizing, which is an integral controlled system specific only to the plant organism. Normally, ROS are generated by metabolic activity of the plants and act as signaling molecules for activating plant metabolic pathway. However, under environmental stresses, generation of ROS increases in different compartments of the cell such as chloroplast, peroxisomes and mitochondria. Higher accumulation of ROS leads to oxidative stress in plant causing damage to the cell membranes (lipid peroxidation) and biomolecules. To combat the harmful effect of increased ROS accumulation, plants are equipped with effective ROS-scavenging mechanisms. Plants have evolved two types of scavenging tools; enzymes (superoxide dismutase (SOD), catalase (CAT), monodehydroascorbate reductase (MDAR), dihydroascorbate reductase (DHAR), glutathione reductase (GR) and glutathione peroxidase (GP)) and antioxidant molecules like ascorbic acid, α—tocopherols, glutathione, prolin, flavonoids and carotenoids. In implementation of these reactions, vacuoles as well as cell wall and plasma membrane also play an important role.