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1. Polymerase chain reaction (PCR) identification of terverticillate Penicillium species isolated from agricultural soils in eskişehir province

Author

Rasime Demirel, Nalan Yilmaz Sariozlu, and Semra İlhan

Subjects
Penicillium, identification methods, PCR, ITS, Biotechnology, TP248.13-248.65
Abstract

In the present study, nine terverticillate Penicillium isolates (P. griseofulfum, P. puberulum, P. crustosum, P. aurantiogriseum, P. chrysogenum, P. primulinum, P. expansum, P. viridicatum, Eupenicillium egyptiacum) from 56 soil samples were characterized genetically by a PCR method. The DNAs of the strains were isolated using the glass beads and vortexing extraction method and then used for PCR amplification with the internal transcribed spacer 1 (ITS1) and ITS4 universal fungal specific primers. The ITS regions of fungal ribosomal DNA (rDNA) were sequenced through the CEQ 8000 Genetic Analysis System. ITS-5.8S sequences obtained were compared with those deposited in the GenBank Database. The results indicated that the identification of Penicillium species with PCR based methods provided significant information about the solution to taxonomy and improve food safety and to protect the users from harmful contaminants such as mycotoxins, which must be controlled during the production of agricultural materials as well as during the processing of food and feed.

Published
2013

2. Mycotoxigenic moulds and mycotoxins in flours consumed in Turkey

Author

Rasime Demirel and Nalan Yılmaz Sarıözlü

Subjects
Ochratoxin A, Aflatoxin, Aspergillus, Nutrition and Dietetics, Microfungi, biology, Mycotoxin contamination, Contamination, biology.organism_classification, chemistry.chemical_compound, chemistry, Penicillium, Botany, Food science, Mycotoxin, Agronomy and Crop Science, Food Science, Biotechnology
Abstract

BACKGROUND Aflatoxins (AFs) and ochratoxin A (OTA) are metabolites produced by several fungi of the genera Aspergillus and Penicillium and have been found to contaminate human foods and animal feeds. The aim of this study was to investigate the abundance and diversity of total microfungi and mycotoxigenic fungi in 25 samples of different grain-based flours from four regions of Turkey (Thrace and Central, Northwest and West Anatolia) and to evaluate the level of AF and OTA contamination. Microscopic and polymerase chain reaction analyses were used to identify fungi, while high-performance liquid chromatography was used for the detection of AFs and OTA. RESULTS A total of 551 fungal strains were obtained from the samples and identified morphologically and by multi-locus gene sequencing. All samples were contaminated with fungi ((2–4.8) × 104 colony-forming units g−1) and three of them exceeded the European Commission (EC) limits. The data also revealed that 70.5 and 14.7% of the fungal isolates were positive for AF and OTA production respectively. In addition, 21 samples were contaminated by AFs (14.98 and 22.4 µg kg−1 for AFB1) and OTA (3.02 and 4.76 µg kg−1) and three of them exceeded the EC limits. CONCLUSION This study is the first report on problems with the occurrence of microfungi, mycotoxin contamination, AFs and OTA in different grain-based flour samples from Turkey and highlights developable points of current limits for food and public health safety. © 2013 Society of Chemical Industry

Published
2013

3. Nanosensors having dipicolinic acid imprinted nanoshell for Bacillus cereus spores detection

Author

Arzu Ersöz, Rıdvan Say, Nalan Yılmaz Sarıözlü, Aytaç Gültekin, Adil Denizli, Anadolu Üniversitesi, Fen Fakültesi, Fizik Bölümü, Ersöz, Arzu, and Say, Rıdvan

Subjects
Dipicolinic Acid, Materials science, Bioengineering, Nanotechnology, Nanoclusters, Gold-Silver Nanoclusters Sensor, chemistry.chemical_compound, Molecularly Imprinted Polymers, Monolayer, General Materials Science, Photoluminescence, Bacillus Cereus Spores Recognition, chemistry.chemical_classification, fungi, Molecularly imprinted polymer, General Chemistry, Polymer, Condensed Matter Physics, Dipicolinic acid, Combinatorial chemistry, Atomic and Molecular Physics, and Optics, Nanoshell, Monomer, chemistry, Modeling and Simulation, Molecular imprinting
Abstract

WOS: 000280240600013, Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP nanoclusters have started to appear in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamido-cysteine (MAC) attached to gold-silver nanoclusters, reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for recognition. In this method, methacryloylamidoantipyrine-terbium ((MAAP)(2)-Tb(III)) has been used as a new metal-chelating monomer via metal coordination-chelation interactions and dipicolinic acid (DPA) which is main participant of Bacillus cereus spores used as a model. Nanoshell sensors with templates give a cavity that is selective for DPA. The DPA can simultaneously chelate to Tb(III) metal ion and fit into the shape-selective cavity. Thus, the interaction between Tb(III) ion and free coordination spheres has an effect on the binding ability of the gold-silver nanoclusters nanosensor. The binding affinity of the DPA imprinted nanoclusters has been investigated by using the Langmuir and Scatchard methods, and the respective affinity constants (K (affinity)) determined were found to be 1.43 x 10(4) and 9.1 x 10(6) mol L(-1).

Published
2009

4. Separation and purification of hyaluronic acid by glucuronic acid imprinted microbeads

Author

Arzu Ersöz, H.Açelya Akdamar, Nalan Yılmaz Sarıözlü, Ayça Atılır Özcan, Rıdvan Say, Adil Denizli, Anadolu Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Ersöz, Arzu, and Say, Rıdvan

Subjects
Chromatography, Materials science, Substrate (chemistry), Bioengineering, Streptococcus Equi, Glucuronic acid, Hyaluronic Acid (Ha), Biomaterials, chemistry.chemical_compound, Monomer, Adsorption, Molecular Imprinted Polymers (Mip), chemistry, Mechanics of Materials, Hyaluronic acid, Amine gas treating, Suspension polymerization, Fourier transform infrared spectroscopy
Abstract

WOS: 000269139900058, The purification of hyaluronic acid (HA) is relatively significant to use in biomedical applications. The structure of HA is formed by the repetitive units of glucuronic acid and N-acetyl glucosamine. In this study, glucuronic acid-imprinted microbeads have been supplied for the purification of HA from cell culture (Streptococcus equi). Histidine-functional monomer. methacryloylamidohistidine (MAH) was chosen as the metal-complexing monomer. The glucuronic acid-imprinted poly(ethyleneglycoldimethacrylate-MAH-Copper(II)) [p(EDMA-MAH-Cu2+)] microbeads have been synthesized by typical suspension polymerization procedure. The template glucuronic acid has been removed by employing 5 M methanolic KOH solution. p (EDMA-MAH-Cu2+) microbeads have been characterized by elemental analysis, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) images and swelling studies. Moreover, HA adsorption experiments have been performed in a batch experimental set-up. Purification of HA from cell culture supernatant has been also investigated by determining the hyaluronidase activity using purified HA as substrate. The glucuronic acid imprinted p(EDMA-MAH-Cu2+) particles can be used many times with no significant loss in adsorption capacities. Also, the selectivity of prepared molecular imprinted polymers (MIP) has been examined. Results have showed that MIP particles are 19 times more selective for glucuronic acid than N-acetylglucose amine

Published
2009

5. Gallnuts (Quercus infectoria Oliv. and Rhus chinensis Mill.) and Their Usage in Health

Author

Nalan Yılmaz Sarıözlü, Merih Kıvanç, Preedy, VR, Watson, RR, Patel, VB, Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, and Kıvanç, Merih

Subjects
chemistry.chemical_classification, Rhus chinensis, Astringent, Traditional medicine, biology, Chemistry, Antimicrobial, biology.organism_classification, chemistry.chemical_compound, Tannin, Gallic acid, Gallotannin, Quercus infectoria, Ellagic acid
Abstract

WOS: 000311222400061, …

Published
2011

6. Gold nanoparticles having dipicolinic acid imprinted nanoshell for Bacillus cereus spores recognition

Author

Arzu Ersöz, Deniz Hür, Rıdvan Say, Adil Denizli, Nalan Yılmaz Sarıözlü, Aytaç Gültekin, Anadolu Üniversitesi, Fen Fakültesi, Fizik Bölümü, Ersöz, Arzu, Hür, Deniz, and Say, Rıdvan

Subjects
Dipicolinic Acid, Materials science, Quenching (fluorescence), Bacillus Spores Recognition, fungi, Molecularly imprinted polymer, Analytical chemistry, General Physics and Astronomy, Nanoparticle, Surfaces and Interfaces, General Chemistry, Condensed Matter Physics, Photochemistry, Dipicolinic acid, Nanoshell, Surfaces, Coatings and Films, chemistry.chemical_compound, chemistry, Molecularly Imprinted Polymers, Colloidal gold, Monolayer, Molecular imprinting, Gold Nanoparticles Sensor, Photoluminescence
Abstract

WOS: 000270679500028, Taking into account the recognition element for sensors linked to molecular imprinted polymers (MIPs), a proliferation of interest has been witnessed by those who are interested in this subject. Indeed, MIP nanoparticles are theme which recently has come to light in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamidocysteine (MAC) attached to gold nanoparticles, reminiscent of a self-assembled monolayer. Furthermore, a surface shell by synthetic host polymers based on molecular imprinting method for recognition has been reconstructed. In this method, methacryloyl iminodiacetic acid-chrome (MAIDA-Cr(III)) has been used as a new metal-chelating monomer via metal coordination-chelation interactions and dipicolinic acid (DPA) which is the main participant of Bacillus cereus spores has been used as a template. Nanoshell sensors with templates produce a cavity that is selective for DPA. The DPA can simultaneously chelate to Cr(III) metal ion and fit into the shape-selective cavity. Thus, the interaction between Cr(III) ion and free coordination spheres has an effect on the binding ability of the gold nanoparticles nanosensor. The interactions between DPA and MIP particles were studied observing fluorescence measurements. DPA addition caused significant decreases in fluorescence intensity because they induced photoluminescence emission from Au nanoparticles through the specific binding to the recognition sites of the crosslinked nanoshell polymer matrix. The binding affinity of the DPA imprinted nanoparticles has been explored by using the Langmuir and Scatchard methods and the analysis of the quenching results has been performed in terms of the Stern-Volmer equation

Published
2009

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