29 results on '"Naoto Matsuyama"'
Search Results
2. Serum selenoprotein P, but not selenium, predicts future hyperglycemia in a general Japanese population
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Swe Mar Oo, Takeshi Urabe, Seiji Kato, Yoshiro Saito, Toshinari Takamura, Naoto Matsuyama, Masatoshi Nakagen, Mutsumi Tanaka, Yuki Kita, Takehiro Kanamori, Hirofumi Misu, Yumie Takeshita, Shuichi Kaneko, Toru Nagano, and Hiroaki Takayama
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0301 basic medicine ,Blood Glucose ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,lcsh:Medicine ,Type 2 diabetes ,Article ,Impaired glucose tolerance ,03 medical and health sciences ,Selenium ,Insulin resistance ,Japan ,Diabetes mellitus ,Internal medicine ,Selenoprotein P ,medicine ,Humans ,lcsh:Science ,Multidisciplinary ,030102 biochemistry & molecular biology ,Receiver operating characteristic ,business.industry ,Insulin ,lcsh:R ,Fasting ,Middle Aged ,medicine.disease ,Prognosis ,030104 developmental biology ,Endocrinology ,Hyperglycemia ,Female ,lcsh:Q ,business ,Homeostasis ,Follow-Up Studies - Abstract
We aimed to test the hypothesis that selenoprotein P (SELENOP), a hepatokine involved in the development of both insulin resistance and impaired insulin production in mice, is related to future onset of hyperglycemia in humans. 76 healthy non-pregnant human subjects without diabetes underwent oral glucose tolerance test (OGTT) at baseline and 4-years follow-up. Nine subjects developed either impaired glucose tolerance or type 2 diabetes at follow-up. At baseline, SELENOP concentrations correlated negatively with insulinogenic index, but not with homeostasis model assessment-estimated insulin resistance (HOMA-IR). Multivariate analysis showed that baseline SELENOP predicted fasting plasma glucose at follow-up independently of the other parameters. The receiver operating characteristic (ROC) curve analysis showed that baseline concentrations of serum SELENOP, but not of selenium, were a reliable test to predict future onset of glucose intolerance. In conclusion, elevation of circulating SELENOP, but not of circulating selenium, was positively and independently associated with future onset of glucose intolerance in a general Japanese population.
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- 2018
3. A novel sol particle immunoassay for fecal calprotectin in inflammatory bowel disease patients
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Yuko Okuyama, Naoto Matsuyama, Takayuki Yamamoto, Yosuke Doi, and Michiyo Uchino
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Adult ,Male ,Time Factors ,Clinical Biochemistry ,Enzyme-Linked Immunosorbent Assay ,Biochemistry ,Inflammatory bowel disease ,Feces ,03 medical and health sciences ,0302 clinical medicine ,Limit of Detection ,medicine ,Humans ,Measurement method ,Chromatography ,medicine.diagnostic_test ,Chemistry ,Biochemistry (medical) ,General Medicine ,Middle Aged ,Inflammatory Bowel Diseases ,medicine.disease ,030220 oncology & carcinogenesis ,Immunoassay ,Calibration ,Immunology ,Linear Models ,Female ,030211 gastroenterology & hepatology ,Calprotectin ,Leukocyte L1 Antigen Complex - Abstract
Background We introduce a new assay method to measure the concentration of fecal calprotectin that can be applied in exclusive analyzers. The assay method uses gold colloidal reagents. In addition, we report performance evaluation results for the new method and the results of comparisons with enzyme-linked immunosorbent assay (ELISA) methods. Methods We evaluated the new method by linearity tests and within-run tests. In addition, we collected specimens from patients with a definitive diagnosis of inflammatory bowel disease (n = 566) and examined them using the new method. The results were compared with those from 2 commercially available ELISA kits. Results In the linearity tests, the correlation coefficients between the measured values and the theoretical values were 0.9980–0.9990. In the within-run tests, the CVs were 3.4–4.3%. The correlation coefficients for our method and the 2 ELISA kits showed high correlations of 0.945 and 0.942. Conclusions Our assay is capable of measuring calprotectin concentrations in feces, and has a similar performance to commercially available ELISA methods. Our method is an automated assay system, which is an easier, cheaper, and quicker measurement method than conventional ELISA kits. Therefore, our assay is suitable for daily clinical use.
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- 2016
4. Pressure Modulation of Backbone Conformation and Intermolecular Distance of Conjugated Polymers Toward Understanding the Dynamism of π-Figuration of their Conjugated System
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Masataka Kumano, Motohiro Nakano, Yuki Noguchi, Naohisa Hirao, Sho Yamanaka, Naoto Matsuyama, Yasuo Ohishi, Shu Seki, Takenori Fujiwara, Tsuneaki Sakurai, and Akinori Saeki
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Organic electronics ,Materials science ,Photoconductivity ,Intermolecular force ,Conjugated system ,Conductivity ,Polymer solar cell ,Diamond anvil cell ,Surfaces, Coatings and Films ,symbols.namesake ,Chemical physics ,Polymer chemistry ,Materials Chemistry ,symbols ,Physical and Theoretical Chemistry ,Raman spectroscopy - Abstract
Continuous tuning of the backbone conformation and interchain distance of a π-conjugated polymer is an essential prerequisite to unveil the inherent electrical and optical features of organic electronics. To this end, applying pressure in a hydrostatic medium or diamond anvil cell is a facile approach without the need for side-chain synthetic engineering. We report the development of high-pressure, time-resolved microwave conductivity (HP-TRMC) and evaluation of transient photoconductivity in the regioregular poly(3-hexylthiophene) (P3HT) film and its bulk heterojunction blend with methanofullerene (PCBM). X-ray diffraction experiments under high pressure were performed to detail the pressure dependence of π-stacking and interlamellar distances in P3HT crystallites and PCBM aggregates. The HP-TRMC results were further correlated with high-pressure Raman spectroscopy and density functional theory calculation. The increased HP-TRMC conductivity of P3HT under pressure was found to be relevant to the planarity of the backbone conformation and intramolecular hole mobility. The effects of pressure on the backbone planarity are estimated to be ∼0.3 kJ mol(-1) based on the compressibility derived from the X-ray diffraction under high pressure, suggesting the high enough energy to cause modulation of the planarity in terms of the Landau-de Gennes free energy of isolated P3HT chains as 0.23 kJ mol(-1). In contrast, the P3HT:PCBM blend showed a simple decrease in photoconductivity irrespective of the identical compressive behavior of P3HT. A mechanistic insight into the interplay of intra- and intermolecular mobilities is a key to tailoring the dynamic π-figuration associated with electrical properties, which may lead to the use of HP-TRMC for exploring divergent π-conjugated materials at the desired molecular arrangement and conformation.
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- 2015
5. Development of a Sol Particle Homogeneous Immunoassay for Measuring Full‐Length Selenoprotein P in Human Serum
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Masatoshi Nakagen, Kazuhiko Takahashi, Yoshiro Saito, Toshinari Takamura, Naoto Matsuyama, Takeshi Urabe, Hirofumi Misu, Shuichi Kaneko, Yuki Kita, Yumie Takeshita, Mutsumi Tanaka, Seiji Kato, Takehiro Kanamori, and Toru Nagano
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0301 basic medicine ,Microbiology (medical) ,medicine.drug_class ,Clinical Biochemistry ,chemistry.chemical_element ,Enzyme-Linked Immunosorbent Assay ,Gold Colloid ,Monoclonal antibody ,03 medical and health sciences ,Limit of Detection ,Selenoprotein P ,medicine ,Humans ,Immunology and Allergy ,Immunoassay ,chemistry.chemical_classification ,Chromatography ,medicine.diagnostic_test ,Hemagglutination ,Biochemistry (medical) ,Public Health, Environmental and Occupational Health ,Anticoagulants ,Original Articles ,Hematology ,Molecular biology ,Medical Laboratory Technology ,030104 developmental biology ,chemistry ,Homogeneous ,Colloidal gold ,Calibration ,Proteolysis ,Particle ,Kallikreins ,Selenoprotein ,Selenium - Abstract
Background Selenoprotein P (SeP), a selenium-rich extracellular glycoprotein, is the primary selenoprotein in the plasma. SeP plays an important role in the maintenance of selenium levels in the peripheral tissues. We developed a new sol particle homogeneous immunoassay (SPIA) for measuring full-length SeP (FL-SeP) levels in the human serum. Methods We used colloidal gold particles coated with two types of anti-SeP monoclonal antibodies, one recognizing the N-terminal side domain of SeP and the other recognizing the C-terminal side domain. Results The assay range was 0.2–9 mg/l, and the linearity was excellent. The within-day and between-day coefficients of variation ranged from 0.73% to 2.24% and 0.45% to 1.11%, respectively. Serum samples (n = 200) were examined using the newly developed assay system (employing a Model 7070 Hitachi automatic clinical analyzer) and the conventional enzyme-linked immunosorbent assay. These two methods were compared using the Passing–Bablok regression analysis; the resulting regression equation and correlation coefficient were y = 0.940x + 0.165 and r = 0.954, respectively. Conclusions Our new SPIA assay is a fully automated homogeneous immunoassay that can be used in conjunction with various commercial analyzers. The assay was sensitive, precise, and suitable for clinical measurement of the FL-SeP in the human serum.
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- 2014
6. Disilanyl Double-Pillared Bisternaphthyl (SiDPBT): Synthesis and Interfused Packing Structures with Herringbone and π-Stack Motifs
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Naoto Matsuyama, Waka Nakanishi, Daisuke Hara, Shu Seki, Hiroyuki Isobe, Akinori Saeki, and Shunpei Hitosugi
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Silicon ,Chemistry ,business.industry ,Organic Chemistry ,Arylene ,chemistry.chemical_element ,General Chemistry ,Crystal engineering ,Biochemistry ,Microwave conductivity ,Crystallography ,Semiconductor ,Stack (abstract data type) ,Molecule ,business ,Single crystal - Abstract
A silacyclophane molecule with two disilanyl pillars and two oligoarylene units was synthesized. The molecule was packed in a single crystal with a new motif interfusing π-stack and herringbone packing structures. The hole transporting ability of the solid was revealed by using the flash-photolysis time-resolved microwave conductivity method. The molecular structure, albeit a singly-bonded arylene macrocycle, was rigidified by the unique packing array, which favorably contributed to the hole transporting ability of the solid via the small reorganization energy through the charge transport.
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- 2014
7. Deficiency of the hepatokine selenoprotein P increases responsiveness to exercise in mice through upregulation of reactive oxygen species and AMP-activated protein kinase in muscle
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Noriko Noguchi, Kazuhiro Takekoshi, Seiji Maeda, Hiroaki Takayama, Seiichi Matsugo, Akihiro Kikuchi, Keita Chikamoto, Shuichi Kaneko, Fei Lan, Yuichiro Mita, Yuya Yoshioka, Takehiro Kanamori, Hirofumi Misu, Kumpei Tokuyama, Yumie Takeshita, Kiyo-aki Ishii, Toshinari Takamura, Nobuhiko Akazawa, Kaito Iwayama, Naoto Matsuyama, Masao Honda, Yoshiro Saito, Mutsumi Tanaka, Natsumi Tajima, and Seiji Kato
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0301 basic medicine ,medicine.medical_specialty ,Muscle Fibers, Skeletal ,Biology ,AMP-Activated Protein Kinases ,General Biochemistry, Genetics and Molecular Biology ,Antioxidants ,03 medical and health sciences ,Mice ,Downregulation and upregulation ,AMP-activated protein kinase ,Internal medicine ,Physical Conditioning, Animal ,Selenoprotein P ,medicine ,Animals ,Humans ,Phosphorylation ,Receptor ,Muscle, Skeletal ,Exercise ,chemistry.chemical_classification ,Mice, Knockout ,Reactive oxygen species ,integumentary system ,Tumor Suppressor Proteins ,AMPK ,General Medicine ,LRP1 ,Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha ,Acetylcysteine ,Up-Regulation ,030104 developmental biology ,Endocrinology ,chemistry ,Receptors, LDL ,biology.protein ,Physical Endurance ,Reactive Oxygen Species ,Low Density Lipoprotein Receptor-Related Protein-1 ,Physical Conditioning, Human - Abstract
Exercise has numerous health-promoting effects in humans; however, individual responsiveness to exercise with regard to endurance or metabolic health differs markedly. This 'exercise resistance' is considered to be congenital, with no evident acquired causative factors. Here we show that the anti-oxidative hepatokine selenoprotein P (SeP) causes exercise resistance through its muscle receptor low-density lipoprotein receptor-related protein 1 (LRP1). SeP-deficient mice showed a 'super-endurance' phenotype after exercise training, as well as enhanced reactive oxygen species (ROS) production, AMP-activated protein kinase (AMPK) phosphorylation and peroxisome proliferative activated receptor γ coactivator (Ppargc)-1α (also known as PGC-1α; encoded by Ppargc1a) expression in skeletal muscle. Supplementation with the anti-oxidant N-acetylcysteine (NAC) reduced ROS production and the endurance capacity in SeP-deficient mice. SeP treatment impaired hydrogen-peroxide-induced adaptations through LRP1 in cultured myotubes and suppressed exercise-induced AMPK phosphorylation and Ppargc1a gene expression in mouse skeletal muscle-effects which were blunted in mice with a muscle-specific LRP1 deficiency. Furthermore, we found that increased amounts of circulating SeP predicted the ineffectiveness of training on endurance capacity in humans. Our study suggests that inhibitors of the SeP-LRP1 axis may function as exercise-enhancing drugs to treat diseases associated with a sedentary lifestyle.
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- 2016
8. Benzobisthiazole as Weak Donor for Improved Photovoltaic Performance: Microwave Conductivity Technique Assisted Molecular Engineering
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Naoto Matsuyama, Yoshiko Koizumi, Masashi Tsuji, Shu Seki, Chakkooth Vijayakumar, and Akinori Saeki
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chemistry.chemical_classification ,Materials science ,Organic solar cell ,business.industry ,Polymer ,Conjugated system ,Conductivity ,Electron acceptor ,Condensed Matter Physics ,Acceptor ,Polymer solar cell ,Electronic, Optical and Magnetic Materials ,Biomaterials ,chemistry ,Electrochemistry ,Optoelectronics ,business ,HOMO/LUMO - Abstract
New donor–acceptor-type copolymers comprised of benzobisthiazole (BBTz) as a weak donor rather than acceptor are proposed. This approach can simultaneously lead to deepening the HOMO and LUMO of the polymers with moderate energy offset against fullerene derivatives in bulk heterojunction organic photovoltaics. As a proof-of-concept, BBTz-based random copolymers conjugated with typical electron acceptors: thienopyrroledione (TPD) and benzothiadiazole (BT) based on density functional theory calculations are synthesized. Laser-flash and Xe-flash time-resolved microwave conductivity (TRMC) evaluations of polymer:[6,6]-phenyl C61 butyric acid methyl ester (PCBM) blends are conducted to screen the feasibility of the copolymers, leading to optimization of processing conditions for photovoltaic device application. According to the TMRC results, alternating BBTz-BT copolymers are designed, exhibiting extended photoabsorption up to ca. 750 nm, deep HOMO (–5.5 to –5.7 eV), good miscibility with PCBM, and inherent crystalline nature. Moreover, the maximized PCE of 3.8%, the top-class among BBTz-based polymers reported so far, is realized in an inverted cell using TiOx and MoOx as the buffer layers. This study opens up opportunities to create low-bandgap polymers with deep HOMO, and shows how the device-less TRMC evaluation is of help for decision-making on judicious molecular design.
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- 2013
9. Telmisartan and N-acetylcysteine Suppress Group V Secretory Phospholipase A2 Expression in TNFα-stimulated Human Endothelial Cells and Reduce Associated Atherogenicity
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Ichiro Ieiri, Masanori Iwase, Takanari Kitazono, Naoto Matsuyama, Yutaka Takata, Kazuo Sonoki, and Shigehiro Ohdo
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Male ,medicine.medical_specialty ,Angiotensin-Converting Enzyme Inhibitors ,Stimulation ,Benzoates ,Antioxidants ,Umbilical vein ,Group V Phospholipases A2 ,chemistry.chemical_compound ,Internal medicine ,Human Umbilical Vein Endothelial Cells ,medicine ,Humans ,RNA, Messenger ,Telmisartan ,Chemokine CCL2 ,Pharmacology ,Angiotensin II receptor type 1 ,Tumor Necrosis Factor-alpha ,Monocyte ,Lysophosphatidylcholines ,Middle Aged ,Atherosclerosis ,Angiotensin II ,Acetylcysteine ,Lipoproteins, LDL ,Lysophosphatidylcholine ,Endocrinology ,medicine.anatomical_structure ,Diabetes Mellitus, Type 2 ,Gene Expression Regulation ,chemistry ,Hypertension ,Benzimidazoles ,Female ,lipids (amino acids, peptides, and proteins) ,Cardiology and Cardiovascular Medicine ,medicine.drug ,Lipoprotein - Abstract
Group V secretory phospholipase A2 (sPLA2-V) hydrolyzes phosphatidylcholine in low-density lipoprotein (LDL) to increase lysophosphatidylcholine (LPC) content. Because in human umbilical vein endothelial cells (HUVEC), tumor necrosis factor alpha (TNFα)-induced sPLA2-V expression, and LPC content in LDL and monocyte chemoattractant protein-1 mRNA were enhanced by incubation of LDL with TNFα-stimulated HUVEC, we investigated whether an angiotensin II receptor type 1 blocker, telmisartan, or an antioxidant drug, N-acetylcysteine (NAC), suppressed TNFα-induced sPLA2-V expression. Telmisartan or NAC administered before and during TNFα stimulation diminished the increase of sPLA2-V mRNA in HUVEC and reduced TNFα-induced sPLA2-V protein at 3 days after TNFα stimulation. Angiotensin II did not induce sPLA2-V mRNA, and a peroxisome proliferator-activated receptor-γ antagonist, GW3335, did not influence the inhibitory effect of telmisartan on TNFα-induced sPLA2-V mRNA. At 3 days after TNFα stimulation, 30 μM telmisartan or 20 mM NAC administered before and during TNFα stimulation prevented the enhancement of LPC content in LDL and monocyte chemoattractant protein-1 mRNA by LDL incubation with TNFα-stimulated HUVEC. A 2-month treatment with telmisartan in 29 hypertensive type 2 diabetic patients significantly reduced LPC content in circulating LDL. Telmisartan's suppressive effect on TNFα-induced sPLA2-V expression may have beneficial effects in preventing proatherogenic changes of LDL.
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- 2012
10. Dehydrogenative Synthesis of C3-Azolylindoles via Copper-Promoted Annulative Direct Coupling of o-Alkynylanilines
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Naoto Matsuyama, Masahiro Miura, Koji Hirano, Tetsuya Satoh, and Yoshiro Oda
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chemistry ,Organic Chemistry ,chemistry.chemical_element ,Direct coupling ,Combinatorial chemistry ,Copper ,Catalysis - Abstract
A copper-promoted annulative direct coupling of o-alkynylaniline derivatives with 1,3,4-oxadiazoles for the synthesis of C3-azolylindoles has been developed. The copper-based system provides a new protocol for the dehydrogenative construction of indole–oxadiazole conjugations from nonhalogenated and nonmetalated starting materials.
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- 2012
11. Ligand-Controlled Cross-Dimerization and -Trimerization of Alkynes under Nickel Catalysis
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Masahiro Miura, Naoto Matsuyama, Tetsuya Satoh, and Hayato Tsurugi
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chemistry.chemical_compound ,chemistry ,Enyne ,Ligand ,Polymer chemistry ,Pyridine ,Regioselectivity ,General Chemistry ,Photochemistry ,Trimethylsilylacetylene ,Diphenylacetylene ,Cyclooctadiene ,Bond cleavage - Abstract
The cross-dimerization of diphenylacetylene with trimethylsilylacetylene via C¯H bond cleavage in the presence of a catalytic amount of bis(cyclooctadiene)nickel [Ni(cod) 2 ] together with a pyridine-based ligand efficiently proceeds to give the corresponding enyne compound with good yield. In contrast, their 1:2 cross-trimerization leading to a dienyne derivative takes place selectively using a triarylphosphine ligand. The regioselective cross-dimerization of some unsymmetrical internal arylalkynes with terminal silylacetylenes is also accomplished using the pyridine ligand.
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- 2008
12. Room Temperature Direct Alkynylation of 1,3,4-Oxadiazoles with Alkynyl Bromides under Copper Catalysis
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Naoto Matsuyama, Tetsuya Satoh, Tomikazu Kawano, Masahiro Miura, and Koji Hirano
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chemistry.chemical_compound ,Transition metal ,chemistry ,Alkynylation ,Organic Chemistry ,Oxadiazole ,chemistry.chemical_element ,Direct coupling ,Photochemistry ,Combinatorial chemistry ,Copper ,Catalysis - Abstract
The direct alkynylation reaction of 1,3,4-oxadiazoles with alkynyl bromides efficiently proceeds in the presence of a copper catalyst at room temperature to create the corresponding heteroaryl-alkynyl linkage in good yields. This direct coupling provides a rapid and convergent access to oxadiazole core pi-conjugated systems.
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- 2010
13. Synthesis and physical properties of a ball-like three-dimensional π-conjugated molecule
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Takahiro Iwamoto, Shigeru Yamago, Eiichi Kayahara, Mamoru Fujitsuka, Toshiyasu Suzuki, Naoto Matsuyama, Nobuhiro Yasuda, Shu Seki, Tetsuro Majima, and Hikaru Takaya
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Multidisciplinary ,Materials science ,Fullerene ,Supramolecular chemistry ,General Physics and Astronomy ,Molecular electronics ,Nanotechnology ,General Chemistry ,Carbon nanotube ,Conjugated system ,Combinatorial chemistry ,General Biochemistry, Genetics and Molecular Biology ,Reductive elimination ,law.invention ,chemistry.chemical_compound ,chemistry ,law ,Molecule ,Organic synthesis - Abstract
Curved π-conjugated molecules with closed and three-dimensional (3D) structures, such as fullerenes and carbon nanotubes, have been the subject of intensive research due to their potential applications in molecular electronics. However, basic molecular skeletons of 3D molecules are limited because of the lack of a rational and selective synthetic method by organic synthesis. Here we report the synthesis of a 3D π-conjugated molecule based on the platinum-mediated assembly of four molecules of a stannylated trisubstituted benzene derivative forming a hexanuclear platinum complex with an octahedral shape, from which reductive elimination of platinum gave the target molecule. As many supramolecular transition metal-ligand complexes with 3D cages and polyhedral structures have been synthesized by self-assembly of ligands and metals, the current assembly/reductive elimination strategy could provide a variety of new 3D π-conjugated molecules with different structures and topologies, which are challenging to obtain using conventional synthetic methods.
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- 2013
14. ChemInform Abstract: Dehydrogenative Synthesis of C3-Azolylindoles via Copper-Promoted Annulative Direct Coupling of o-Alkynylanilines
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Koji Hirano, Yoshiro Oda, Naoto Matsuyama, Masahiro Miura, and Tetsuya Satoh
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Bearing (mechanical) ,law ,Chemistry ,Leaving group ,chemistry.chemical_element ,Organic chemistry ,Direct coupling ,General Medicine ,Nitrogen ,Copper ,law.invention - Abstract
o-Alkynylanilines bearing a suitable leaving group (generally o-fluorobenzoyl) at the nitrogen are coupled with oxadiazoles to produce N-unsubstituted indoles directly.
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- 2012
15. New Enzymatic Colorimetric Assay for Total Homocysteine
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Koji Mizuno, Masafumi Yamaguchi, Mitsuyoshi Toyosato, Masaharu Takayama, and Naoto Matsuyama
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D-Amino-Acid Oxidase ,chemistry.chemical_classification ,Biochemistry (medical) ,Clinical Biochemistry ,Alcohol ,Methyltransferases ,Reductase ,Homocysteine S-Methyltransferase ,Blood proteins ,Dithiothreitol ,chemistry.chemical_compound ,Enzyme ,Biochemistry ,chemistry ,Humans ,Colorimetry ,Indicators and Reagents ,Thermolabile ,Homocysteine ,Methylene blue ,Cysteine - Abstract
In 1969, McCully (1) observed that increased plasma homocysteine (Hcy) was linked with vascular disease. Subsequent studies demonstrated that even mild hyperhomocysteinemia is an independent risk factor for cardiovascular, cerebrovascular, and peripheral vascular disease (2)(3)(4)(5)(6)(7)(8). Increased plasma Hcy is associated with a thermolabile variant of 5,10-methylenetetrahydrofolate reductase, smoking, lack of exercise, and excessive use of alcohol and coffee. Plasma or serum total Hcy (tHcy) concentrations are most commonly measured by HPLC (9), which is time- consuming and expensive, and by immunochemical (10)(11)(12) or enzymatic (13) methods, which may not be applicable to all colorimetric-based clinical chemistry analyzers. The thiol group of Hcy allows it to form a disulfide bond with other thiol-containing molecules, such as Hcy itself, cysteine, and the cysteine residue of plasma proteins. Biologic fluids may often contain both reduced and oxidized species of Hcy, and the sum of all the forms of Hcy is usually called total Hcy (tHcy) (14). Most clinical studies concerning Hcy have relied on the measurement of tHcy. An initial chemical reduction step of the sample is inevitable in the tHcy assay. Because reducing agents can interfere with the oxidation of redox indicators, such as Trinder’s reagents and derivatives of methylene blue generally used in diagnostic reagents, methods for tHcy that use these indicators have not been developed. The present method is a new enzymatic colorimetric assay for tHcy in biologic samples. The principle is as follows. In the first step, samples are reduced by dithiothreitol …
- Published
- 2001
16. ChemInform Abstract: Nickel- and Copper-Catalyzed Direct Alkynylation of Azoles and Polyfluoroarenes with Terminal Alkynes under O2 or Atmospheric Conditions
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Naoto Matsuyama, Koji Hirano, Masanori Kitahara, Masahiro Miura, and Tetsuya Satoh
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Nickel ,Alkynylation ,Terminal (electronics) ,Chemistry ,Polymer chemistry ,Copper catalyzed ,chemistry.chemical_element ,General Medicine ,Oxygen ,Coupling reaction ,Catalysis - Abstract
Direct cross coupling reaction of azoles, e.g. (I) and (IV), with terminal alkynes is smoothly achieved using a Ni(II)—bipyridine catalyst under oxygen.
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- 2010
17. ChemInform Abstract: Room Temperature Direct Alkynylation of 1,3,4-Oxadiazoles with Alkynyl Bromides under Copper Catalysis
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Koji Hirano, Naoto Matsuyama, Masahiro Miura, Tomikazu Kawano, and Tetsuya Satoh
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chemistry.chemical_compound ,Alkynylation ,Chemistry ,Oxadiazole ,chemistry.chemical_element ,Direct coupling ,General Medicine ,Copper ,Combinatorial chemistry ,Catalysis - Abstract
The direct alkynylation reaction of 1,3,4-oxadiazoles with alkynyl bromides efficiently proceeds in the presence of a copper catalyst at room temperature to create the corresponding heteroaryl−alkynyl linkage in good yields. This direct coupling provides a rapid and convergent access to oxadiazole core π-conjugated systems.
- Published
- 2010
18. Nickel- and copper-catalyzed direct alkynylation of azoles and polyfluoroarenes with terminal alkynes under O(2) or atmospheric conditions
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Masahiro Miura, Naoto Matsuyama, Masanori Kitahara, Koji Hirano, and Tetsuya Satoh
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Azoles ,Molecular Structure ,Chemistry ,Acetylene ,Atmosphere ,Organic Chemistry ,Sonogashira coupling ,chemistry.chemical_element ,Stereoisomerism ,Photochemistry ,Biochemistry ,Copper ,Combinatorial chemistry ,Catalysis ,Fluorobenzenes ,Oxygen ,Nickel ,Alkynylation ,Terminal (electronics) ,Alkynes ,Copper catalyzed ,Physical and Theoretical Chemistry - Abstract
The direct C-H alkynylation of azoles with terminal alkynes proceeds efficiently under a nickel/O(2) catalytic system. On the other hand, a copper/air catalyst enables the coupling of polyfluoroarenes with terminal alkynes. These catalyses provide new accesses to arylacetylenes through the formal direct Sonogashira coupling.
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- 2010
19. ChemInform Abstract: Nickel-Catalyzed Direct Alkynylation of Azoles with Alkynyl Bromides
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Naoto Matsuyama, Koji Hirano, Masahiro Miura, and Tetsuya Satoh
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chemistry.chemical_classification ,Silylation ,Aryl ,chemistry.chemical_element ,General Medicine ,Combinatorial chemistry ,Catalysis ,Nickel ,chemistry.chemical_compound ,chemistry ,Alkynylation ,Azole ,Direct coupling ,Alkyl - Abstract
The direct C−H alkynylation of azoles with alkynyl bromides proceeds efficiently in the presence of a nickel-based catalyst system. The reaction enables the introduction of various alkynyl groups bearing aryl, alkenyl, alkyl, and silyl substituents to the azole cores. In some cases, addition of a catalytic amount of CuI is observed to accelerate the direct coupling dramatically.
- Published
- 2010
20. ChemInform Abstract: Nickel- and Rhodium-Catalyzed Addition of Terminal Silylacetylenes to Propargyl Amines: Catalyst-Dependent Complementary Regioselectivity
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Tetsuya Satoh, Koji Hirano, Naoto Matsuyama, and Masahiro Miura
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Nickel ,Chemistry ,Polymer chemistry ,Propargyl ,Organic chemistry ,chemistry.chemical_element ,Regioselectivity ,General Medicine ,Cleavage (embryo) ,Catalysis ,Rhodium - Abstract
The cross-addition of terminal silylacetylenes to γ-arylated propargyl amines occurs efficiently via C−H cleavage by using either a nickel or rhodium catalyst. Taking advantage of the catalyst-cont...
- Published
- 2009
21. Total oxidation of toluene on Pt/CeO2-ZrO2-Bi2O3/gamma-Al2O3 catalysts prepared in the presence of polyvinyl pyrrolidone
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Nobuhito Imanaka, Hayato Imadzu, Toshiyuki Masui, and Naoto Matsuyama
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Boron Compounds ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Analytical chemistry ,chemistry.chemical_element ,Catalytic combustion ,Incineration ,Catalysis ,chemistry.chemical_compound ,Specific surface area ,Aluminum Oxide ,Environmental Chemistry ,Waste Management and Disposal ,Platinum ,Air Pollutants ,Volatile Organic Compounds ,Thermal conductivity detector ,Cerium ,Pollution ,Toluene ,Toluene oxidation ,Pyrrolidinones ,chemistry ,Polyvinyls ,Zirconium ,Bismuth ,Oxidation-Reduction ,Nuclear chemistry ,Space velocity - Abstract
Pt/CeO(2)-ZrO(2)-Bi(2)O(3)/gamma-Al(2)O(3) (Pt/CZB/Al(2)O(3)) catalysts for the catalytic combustion of toluene, which is one of the volatile organic compounds (VOCs), were prepared by the wet impregnation method in the presence of polyvinyl pyrrolidone (PVP). X-ray powder diffraction, transmission electron microscopy, and BET specific surface area measurement using N(2) adsorption have been used to characterize the catalysts. The catalytic test was conducted from room temperature in a flow of 900 ppm of toluene in air and gas hourly space velocity (GHSV) of 8000 h(-1). The catalytic activity was evaluated in terms of C(7)H(8) conversion and the gas composition after the reaction was analyzed using two gas chromatographs with a flame ionization detector (FID) and a thermal conductivity detector (TCD). The Pt/CZB/Al(2)O(3) catalysts are specific for the total toluene oxidation and CO and any toluene-derivative compounds were not detected as by-products. The specific surface area of the catalysts was increased by the addition of PVP in the preparation process. By the optimization of the amount of platinum, complete oxidation of toluene was realized at a temperature as low as 120 degrees C on a 7 wt%Pt/16 wt%Ce(0.64)Zr(0.15)Bi(0.21)O(1.895)/gamma-Al(2)O(3) catalyst.
- Published
- 2009
22. Nickel- and rhodium-catalyzed addition of terminal silylacetylenes to propargyl amines: catalyst-dependent complementary regioselectivity
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Koji Hirano, Naoto Matsuyama, Masahiro Miura, and Tetsuya Satoh
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Nickel ,Transition metal ,chemistry ,Organic Chemistry ,Propargyl ,Polymer chemistry ,Regioselectivity ,chemistry.chemical_element ,Amine gas treating ,Cleavage (embryo) ,Catalysis ,Rhodium - Abstract
The cross-addition of terminal silylacetylenes to gamma-arylated propargyl amines occurs efficiently via C-H cleavage by using either a nickel or rhodium catalyst. Taking advantage of the catalyst-controlled switching of regioselectivity in the reaction, both the 2- and 3-alkynylallylamines are readily accessible from the same starting materials.
- Published
- 2009
23. ChemInform Abstract: Ligand-Controlled Cross-Dimerization and -Trimerization of Alkynes under Nickel Catalysis
- Author
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Masahiro Miura, Tetsuya Satoh, Naoto Matsuyama, and Hayato Tsurugi
- Subjects
chemistry.chemical_compound ,chemistry ,Enyne ,Ligand ,Polymer chemistry ,Pyridine ,Regioselectivity ,General Medicine ,Diphenylacetylene ,Trimethylsilylacetylene ,Bond cleavage ,Cyclooctadiene - Abstract
The cross-dimerization of diphenylacetylene with trimethylsilylacetylene via C¯H bond cleavage in the presence of a catalytic amount of bis(cyclooctadiene)nickel [Ni(cod) 2 ] together with a pyridine-based ligand efficiently proceeds to give the corresponding enyne compound with good yield. In contrast, their 1:2 cross-trimerization leading to a dienyne derivative takes place selectively using a triarylphosphine ligand. The regioselective cross-dimerization of some unsymmetrical internal arylalkynes with terminal silylacetylenes is also accomplished using the pyridine ligand.
- Published
- 2009
24. Relations of lysophosphatidylcholine in low-density lipoprotein with serum lipoprotein-associated phospholipase A2, paraoxonase and homocysteine thiolactonase activities in patients with type 2 diabetes mellitus
- Author
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Masanori Iwase, Shun Higuchi, Kazuo Sonoki, Naoto Matsuyama, Nobuhiro Sasaki, Shigehiro Ohdo, and Mitsuo Iida
- Subjects
Adult ,Male ,medicine.medical_specialty ,Spectrometry, Mass, Electrospray Ionization ,Homocysteine ,Endocrinology, Diabetes and Metabolism ,Blood Pressure ,Body Mass Index ,Diabetic nephropathy ,chemistry.chemical_compound ,Endocrinology ,Reference Values ,Internal medicine ,Diabetes mellitus ,Lecithins ,Internal Medicine ,medicine ,Humans ,biology ,business.industry ,Aryldialkylphosphatase ,Lipoprotein-associated phospholipase A2 ,Cholesterol, HDL ,Paraoxonase ,Type 2 Diabetes Mellitus ,Lysophosphatidylcholines ,General Medicine ,Cholesterol, LDL ,respiratory system ,Middle Aged ,medicine.disease ,Lipoproteins, LDL ,Phospholipases A2 ,chemistry ,Diabetes Mellitus, Type 2 ,Simvastatin ,Low-density lipoprotein ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Female ,business ,Carboxylic Ester Hydrolases ,medicine.drug - Abstract
Aims We studied the relations of lysophosphatidylcholine (lyso-PC) in LDL with serum lipoprotein-associated phospholipase A 2 (Lp-PLA 2 ), paraoxonase and homocysteine thiolactonase (HTLase) activities in patients with type 2 diabetes mellitus. Methods Lyso-PC was measured by electrospray ionization-liquid chromatography/mass spectrometry. Paraoxonase and HTLase activities were measured with paraoxon and γ-thiobutyrolactone as substrates, respectively. Results Serum HTLase and paraoxonase activities were significantly suppressed in diabetic patients ( n = 96) compared with control ( n = 25), whereas serum Lp-PLA 2 did not differ in control and diabetic patients. Lyso-PC contents in LDL correlated with serum Lp-PLA 2 activity positively and with serum HTLase activity negatively. Stepwise regression analysis revealed that serum Lp-PLA 2 and HTLase activities independently contributed to lyso-PC contents in LDL. In patients with diabetic nephropathy, lyso-PC contents in LDL were increased with reduced serum HTLase and paraoxonase activities compared with control, while serum Lp-PLA 2 activity did not differ. On the other hand, 3-month treatment with simvastatin reduced both lyso-PC contents in LDL and serum Lp-PLA 2 activity in hypercholesterolemic diabetic patients, while serum HTLase or paraoxonase activities did not change. Conclusions Increased lyso-PC contents in LDL were associated with the suppressed HTLase activity, and serum Lp-PLA 2 and HTLase activities may be related to lyso-PC in type 2 diabetic patients.
- Published
- 2009
25. Organic Photovoltaics: Benzobisthiazole as Weak Donor for Improved Photovoltaic Performance: Microwave Conductivity Technique Assisted Molecular Engineering (Adv. Funct. Mater. 1/2014)
- Author
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Naoto Matsuyama, Akinori Saeki, Yoshiko Koizumi, Chakkooth Vijayakumar, Masashi Tsuji, and Shu Seki
- Subjects
Biomaterials ,Microwave conductivity ,Materials science ,Organic solar cell ,business.industry ,Photovoltaic system ,Electrochemistry ,Optoelectronics ,Condensed Matter Physics ,business ,Electronic, Optical and Magnetic Materials ,Molecular engineering - Published
- 2014
26. Polyunsaturated fatty acid anilides as inhibitors of acyl-coA: cholesterol acyltransferase (ACAT)
- Author
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Koji Mizuno, Naoto Matsuyama, Mina Fukuhara, Yasuji Soda, and Tetsuya Kosaka
- Subjects
Docosahexaenoic Acids ,Clinical Biochemistry ,Sterol O-acyltransferase ,Drug Evaluation, Preclinical ,Pharmaceutical Science ,Biochemistry ,Cell Line ,chemistry.chemical_compound ,Inhibitory Concentration 50 ,Dogs ,Species Specificity ,Microsomes ,Drug Discovery ,Adrenal Glands ,medicine ,Animals ,Humans ,Anilides ,Enzyme Inhibitors ,Molecular Biology ,chemistry.chemical_classification ,biology ,Cholesterol ,Anticholesteremic Agents ,Macrophages ,Organic Chemistry ,Intestines ,Enzyme ,medicine.anatomical_structure ,chemistry ,Eicosapentaenoic Acid ,Liver ,Enzyme inhibitor ,Acyltransferases ,Hepatocyte ,biology.protein ,Microsome ,Fatty Acids, Unsaturated ,Molecular Medicine ,Rabbits ,Polyunsaturated fatty acid ,Sterol O-Acyltransferase - Abstract
A series of polyunsaturated fatty acid anilides were synthesized and evaluated as ACAT inhibitors. Compound 24 had potent inhibitory activity against microsomal ACAT derived from U937, HepG2 and Caco-2 cell lines. Therefore, it might be expected to act as an antiarteriosclerotic and hypocholesterolemic agent. Interestingly, the ACAT inhibitory potency of 24 varied significantly depending on the source of the enzyme.
- Published
- 1999
27. Pressure Modulation of Backbone Conformation and IntermolecularDistance of Conjugated Polymers Toward Understanding the Dynamismof π-Figuration of their Conjugated System.
- Author
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Yuki Noguchi, Akinori Saeki, Takenori Fujiwara, Sho Yamanaka, Masataka Kumano, Tsuneaki Sakurai, Naoto Matsuyama, Motohiro Nakano, Naohisa Hirao, Yasuo Ohishi, and Shu Seki
- Published
- 2015
- Full Text
- View/download PDF
28. Nickel- and Copper-Catalyzed Direct Alkynylation of Azoles and Polyfluoroarenes with Terminal Alkynes under O2or Atmospheric Conditions.
- Author
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Naoto Matsuyama, Masanori Kitahara, Koji Hirano, Tetsuya Satoh, and Masahiro Miura
- Subjects
- *
TRANSITION metal catalysts , *AZOLES , *POLYFLUORENES , *ALKYNES , *OXYGEN , *CHEMICAL bonds , *ACETYLENE , *CHEMICAL reactions - Abstract
The direct C−H alkynylation of azoles with terminal alkynes proceeds efficiently under a nickel/O2catalytic system. On the other hand, a copper/air catalyst enables the coupling of polyfluoroarenes with terminal alkynes. These catalyses provide new accesses to arylacetylenes through the formal direct Sonogashira coupling. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
29. Nickel-Catalyzed Direct Alkynylation of Azoles with Alkynyl Bromides.
- Author
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Naoto Matsuyama, Koji Hirano, Tetsuya Satoh, and Masahiro Miura
- Subjects
- *
NICKEL catalysts , *AZOLES , *BROMIDES , *CHEMICAL bonds , *ADDITION reactions , *CUPROUS iodide - Abstract
The direct C−H alkynylation of azoles with alkynyl bromides proceeds efficiently in the presence of a nickel-based catalyst system. The reaction enables the introduction of various alkynyl groups bearing aryl, alkenyl, alkyl, and silyl substituents to the azole cores. In some cases, addition of a catalytic amount of CuI is observed to accelerate the direct coupling dramatically. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
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