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306 results on '"Nap, Jan Peter"'

9. Towards recommendations for metadata and data handling in plant phenotyping

Author

Krajewski, Paweł, Chen, Dijun, Ćwiek, Hanna, van Dijk, Aalt D.J., Fiorani, Fabio, Kersey, Paul, Klukas, Christian, Lange, Matthias, Markiewicz, Augustyn, Nap, Jan Peter, van Oeveren, Jan, Pommier, Cyril, Scholz, Uwe, van Schriek, Marco, Usadel, Björn, and Weise, Stephan

Published
2015

25. Assembly of two transgenes in an artificial chromatin domain gives assembly highly coordinated expression in tobacco

Author

Mlynarova, Ludmila, Loonen, Annelies, Mietkiewska, Elzbieta, Jansen, Ritsert C., and Nap, Jan-Peter

Subjects
Tobacco -- Genetic aspects, Chromatin -- Research, Gene expression -- Research, Genetically modified plants -- Research, Biological sciences
Abstract

The chromatin loop model predicts that genes within the same chromatin domain exhibit coordinated regulation. We here present the first direct experimental support for this model in plants. Two reporter genes, the E. coli [beta]-glucuronidase gene and the firefly luciferase gene, driven by different promoters, were placed between copies of the chicken lysozyme A element, a member of the matrix-associated region (MAR) group of chromatin boundary elements, and introduced in tobacco (Nicotiana tabacum). In plants carrying A elements, quantitative enzyme activities and mRNA levels of both genes show high correlations compared to control plants. The A element thus creates an artificial chromatin domain that yields coordinated expression. Surprisingly, enzyme activities correlated poorly with their respective mRNA levels. We hypothesize that this indicates the occurrence of 'error pipelines' in data generation: systematic errors of a given analytical method will point in the same direction and cancel out in correlation analysis, resulting in better correlations. In combining different methods of analysis, however, such errors do not cancel out and as a result relevant correlations can be masked. Such error pipelines will have to be taken into account when different types of (e.g., whole-genome) data sets are combined in quantitative analyses.

Published
2002

33. Laudatio Promotie Sven Warris

Author

Nap, Jan Peter

Subjects
Biofysica, Science, Bioinformatica, Biophysics, Computertechnologie
Published
2019

34. Startbijeenkomst WCM

Author

Hofstede, Gert, Faber, Folkert, Zwart, Kor, and Nap, Jan Peter

Subjects
Energy, Rumen, Alternative Gasses And Hybrid Fuels, Lignine, Science, Methaan, Weggras, Hernieuwbare Energie, Duurzaamheid En Het Milieu, Renewable Energy, Sustainability And The Environment, Professional Practice &Amp; Society, Vfa, Lignin, Education, Pens, Alternatieve Gassen En Hybride Brandstoffen, Roadgras, Arr, Energie, Methane
Abstract

Startbijeenkomst WCM

Published
2019

35. Slotbijeenkomst Bio-P2G

Author

Nap, Jan Peter, Hofstede, Gert, Faber, Folkert, and Bekkering, Jan

Subjects
Chemische Technologie (Diversen), Energy, Groen Gas, Energietransitie, Alternative Gasses And Hybrid Fuels, Hybrid Energy System Design And Management, Science, Energy Engineering And Power Technology, Methaan, Energy Transition, Hernieuwbare Energie, Duurzaamheid En Het Milieu, Renewable Energy, Sustainability And The Environment, Professional Practice &Amp; Society, Education, Biologische Methanisering, Ontwerp En Beheer Van Hybride Energie Systemen, Energieopslag, Energietechniek En Krachttechnologie, Duurzaamheid, Alternatieve Gassen En Hybride Brandstoffen, Hybride Energie Systemen, Energie, Chemical Engineering (Miscellaneous)
Abstract

Ter afsluiting van het Bio-P2G project is een eindsymposium georganiseerd waarbij terug wordt geblikt op de afgelopen 4 jaar. De resultaten werden besproken maar ook samenwerkingspartners verzorgden presentaties rondom de energietransitie en biologische methanisering.

Published
2019

36. Biomethane from hydrogen and carbon dioxide

Author

Nap, Jan Peter, Bekkering, Jan, Hofstede, Gert, Faber, Folkert, Wedema, Ronald, Zwart, Kor, Lammers, Gerard, Life Sciences & Renewable Energy, and ALIFE

Subjects
bio energy, energy transition, kooldioxide, energy storage, methane, hydrogen, carbon dioxide, alternative gasses, waterstof, hybrid energy system, energietransitie
Abstract

The project BioP2M came to a close in June 2019 after a consortium of stakeholders in the field of energy transition worked together to research the diverse role of Methane. In this report the results are presented and future plans are discussed.

Published
2019

37. Improving the sustainability of anaerobic digestion through the use of green hydrogen integration for increased green gas production through the use of a symbiotic approach

Author

Pierie, Frank, Bekkering, Jan, Nap, Jan Peter, Energy Transition, and Life Sciences & Renewable Energy

Subjects
energy transition, biogas, energietransitie
Abstract

One potential renewable energy resource is green gas production through anaerobic digestion (AD). However, only part of the biogas produced (up to 50-60%) contains the combustible methane; the remainder are incombustible gasses with the biggest being carbon dioxide. These gasses are often not used and expelled in the atmosphere. Through the use of BIO-P2M where hydrogen is mixed with the remaining CO2 additional methane can be produced, increasing the yield and using the feedstocks more effectively. Within this research the environmental sustainability and effectiveness of BIO-P2M is evaluated using the MEFA and aLCA method, expressed in; net green gas production, efficiency in (P)EROI, emissions in GWP100, and environmental impact in Ecopoints. The functional unit is set as a normal cubic meter of Groningen quality natural gas. Results indicate a net improvement of all indicators when applying BIO-P2M in several configurations (in situ, ex situ). When allocating the production of renewable energy to the BIO-P2M system environmental impacts for wind the results are still positive; however, when using solar PV as an energy source the environmental impact in Ecopoints exceeds that of the reference case of Groningen natural gas. An additional option for improving the indicators is optimization of the process. When using BIO-P2M combined with heat and power unit for producing the internal electricity and heat demands all indicators are improved substantially. On a national scale when utilizing al available waste materials for the BIO-P2M system around 1217 MNm3/a of green gas can be produced, which is 3% of the total yearly consumption in the Netherlands and around 60% more than when using normal AD systems. Within the context BIO-P2M is an interesting option for increasing green gas output and improving the overall sustainability of the AD process. However, the source of green electricity needs to be taken into account and process optimization can ensure better environmental performance.

Published
2019

42. Dissection of a synthesized quantitative trait to characterize transgene interactions

Author

Nap, Jan-Peter, Conner, Anthony J., Mlynarova, Ludmila, Stiekema, Willem J., and Jansen, Ritsert C.

Subjects
Genetically modified plants -- Analysis, Biological sciences
Abstract

Six transgenic tobacco lines, each homozygous for the [Beta]-glucuronidase (GUS) gene at a different locus, and wild type were selfed and intercrossed to evaluate GUS activity in all possible hemizygous, homozygous and dihybrid combinations of GUS alleles. The transgenic lines are characterized by their GUS activity (two low, three intermediate, one high), T-DNA complexity (four single-copy, two more complex single-locus) and the presence of the chicken lysozyme matrix-associated region (MAR) around the full T-DNA (two lines). Gene action and interaction was analyzed by weighted linear regression with parameters for additivity, dominance and epistasis. The analysis showed that each of the four single-copy lines acted fully additively. In contrast, the two complex single-locus lines showed classical single-locus overdominance and were epistatic dominant over all other GUS alleles. The latter is manifested in severe suppression of GUS activity in dihybrid lines, irrespective of the presence of MAR elements around the GUS gene. Such elements apparently do not protect against epistatic dominance. The quantitative data suggested that the epistatic dominance and overdominance are based on the same molecular mechanism. Our approach of a genetic analysis of quantitative variation in well-characterized transgenic lines provides a powerful tool to gain insight into complex plant traits.

Published
1997

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