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7 results on '"Nara, Seia"'

1. Design of an Artificial Peptide Inspired by Transmembrane Mitochondrial Protein for Escorting Exogenous DNA into the Mitochondria to Restore their Functions by Simultaneous Multiple Gene Expression (Adv. Funct. Mater. 8/2024)

Author

Yoshinaga, Naoto, primary, Miyamoto, Takaaki, additional, Odahara, Masaki, additional, Takeda‐Kamiya, Noriko, additional, Toyooka, Kiminori, additional, Nara, Seia, additional, Nishimura, Haruna, additional, Ling, Feng, additional, Su'etsugu, Masayuki, additional, Yoshida, Minoru, additional, and Numata, Keiji, additional

Published
2024
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2. Design of an Artificial Peptide Inspired by Transmembrane Mitochondrial Protein for Escorting Exogenous DNA into the Mitochondria to Restore their Functions by Simultaneous Multiple Gene Expression

Author

Yoshinaga, Naoto, primary, Miyamoto, Takaaki, additional, Odahara, Masaki, additional, Takeda‐Kamiya, Noriko, additional, Toyooka, Kiminori, additional, Nara, Seia, additional, Nishimura, Haruna, additional, Ling, Feng, additional, Su'etsugu, Masayuki, additional, Yoshida, Minoru, additional, and Numata, Keiji, additional

Published
2023
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7. In vitroamplification of whole large plasmids via transposon-mediated oriCinsertion

Author

Nara, Seia and Su'etsugu, Masayuki

Abstract

DNA amplification is a fundamental technique in molecular biology. The replication cycle reaction is a new method for amplification of large circular DNA having oriCsequences, which is a replication initiation site of the Escherichia colichromosome. We here developed a replication cycle reaction-based method useful for amplification of various circular DNAs lacking oriC, even in the absence of any sequence information, via transposon-mediated oriCinsertion to the circular DNA template. A 15-kb non-oriCplasmid was amplified from a very small amount of starting DNA (50 fg, 1 fM). The method was also applicable to GC-rich plasmid (69%) or large F-plasmid (230 kb). This method thus provides a powerful tool to amplify various environmental circular DNAs.

Published
2021
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