Search

Surface plasmon resonance (SPR) is a key technique in developing sensor platforms for clinical diagnostics, drug discovery, food quality, and environmental monitoring applications. While prism‐coupled (Kretschmann) SPR remains a "gold‐standard" for laboratory work‐flows due to easier fabrication, handling and high through put, other configurations such as grating‐coupled SPR (GC‐SPR) and wave‐guide mode SPR are yet to fulfil their technology potential. This work evaluates the technical aspects influencing the performance of GC‐SPR and reviews recent progress in the fabrication of such platforms. In principle, the GC‐SPR involves the illumination of the plasmonic metal film with periodic gratings to excite the surface plasmons (SP) via diffraction‐based phase matching. The real performance of the GC‐SPR is, however, heavily influenced by the topography of the grating structures produced via top‐down lithography techniques. This review discusses latest in approaches to achieve consistent plasmonic gratings with uniform features and periodicity over a large scale and explores the choice of plasmon‐active and substrate material for enhanced performance. The review also provides insights into the different GC‐SPR measurement configurations and highlights on opportunities with their potential applications as biosensors with translational capabilities. [ABSTRACT FROM AUTHOR]

This article offers information on a study which examined the use of recombinant hexon protein as a serodiagnostic agent in rapid latex agglutination test for fowl adenovirus serotype 4 (FAV4). This virus is said to be the cause of hydropericardium hepatitis syndrome (HPS), an emerging poultry disease first observed in a broiler flock in Pakistan during 1987. As explained, the development of a recombinant antigen against the hexon gene of FAV4 prevents the cross reaction with other serotypes of fowl adenovirus.

The article presents a study which examines the utilization of Flinders Technology Associates (FTA)-polymerase chain reaction (PCR) for the molecular detection of Newcastle disease virus (NDV). Researchers used FTA filter papers for the storage of NDV, infected allantoic fluid (AF) and tissue samples. They observed that NDV was inactivated when its comes to contact with FTA. They concluded that the virus inactivated by FTA is an effective source of viral RNA for molecular detection and characterization.