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7. GM Technology and Fiber Traits

Author

Latif, Ayesha, Ahad, Ammara, Ahmed, Mukhtar, Akhtar, Sidra, Yasmeen, Aneela, Shahid, Ahmad Ali, Rao, Abdul Qayyum, Nasir, Idrees Ahmad, Husnain, Tayyab, Rahman, Mehboob-ur-, editor, Zafar, Yusuf, editor, and Zhang, Tianzhen, editor

Published
2021
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20. Biotechniques

Author

Tabassum, Bushra, primary, Nasir, Idrees Ahmad, additional, and Husnain, Tayyab, additional

Published
2018
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25. Science Behind Cotton Transformation

Author

Rao, Abdul Qayyum, primary, Ali, Muhammad Azam, additional, Khan, Muhammad Azmat Ullah, additional, Bajwa, Kamran Shahzad, additional, Iqbal, Adnan, additional, Iqbal, Tahir, additional, Shahid, Ahmad Ali, additional, Nasir, Idrees Ahmad, additional, and Husnain, Tayyab, additional

Published
2016
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28. Genomics of Salinity Tolerance in Plants

Author

Rao, Abdul Qayyum, primary, ud Din, Salah, additional, Akhtar, Sidra, additional, Sarwar, Muhammad Bilal, additional, Ahmed, Mukhtar, additional, Rashid, Bushra, additional, Khan, Muhammad Azmat Ullah, additional, Qaisar, Uzma, additional, Shahid, Ahmad Ali, additional, Nasir, Idrees Ahmad, additional, and Husnain, Tayyab, additional

Published
2016
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35. Comparative Silencing Effect of Different siRNA Fragments on Potato Virus X Coat Protein in Transient Transfection Assays.

Author

Khan, Anwar, Nosheen, Fareeha, Tabassum, Bushra, Adeyinka, Olawale Samuel, Shehzad, Khurram, Shahid, Naila, Khan, Arif Muhammad, and Nasir, Idrees Ahmad

Abstract

Coat protein gene of potato virus X is a structural protein that plays a vital role in viral transmission and pathogenesis. RNA interference or post transcriptional gene silencing is a regulatory conserved mechanism that uses small interfering RNAs to control the expression of desired gene by inhibiting mRNA transcription. The present study aims to investigate the potential role of different siRNAs in down-regulating the mRNA expression of potato virus X in cell culture assays. Three specific siRNAs against CP-PVX gene were designed in which siRNA 3 significantly reduced the CP-PVX mRNA expression in HepG 2 cells upto 91.91% (12.5 fold) as compared to siRNA 1 and siRNA 2 where knockdown percentages were only 20 and 73.04%, respectively as revealed by realtime PCR. Maximum knockdown values were obtained at 100 nM concentration of siRNA 1 and 2, while for siRNA 3 promising knockdown was obtained at 50 nM concentration. For long lasting effect of siRNAs, short hairpin RNA corresponding to siRNA3 was designed and tested against CP-PVX which resulted in a similar pattern of inhibition on RNA expression of target gene as synthetic siRNA3. It was found that mRNA expression of targeted gene was reduced to 91% depicting strong RNAi response generated by shRNA. Our findings support the possibility of using consensus siRNA and shRNA-based approach as a promising strategy in effective inhibition of PVX in in-vitro assay. Down regulation of potato virus X through coat protein gene targeted siRNA is a potential way of inhibiting invading virus and to effectively stop its systemic spread. [ABSTRACT FROM AUTHOR]

Published
2022
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39. IDENTIFICATION OF NOVEL QTLS CONTROLLING SUGARCANE SMUT RESISTANCE AND YIELD TRAITS.

Author

NAZAR, Zaheer Ahmad, KHAN, Hafiz Muhammad Walayat Ali, ALI, Qurban, and NASIR, Idrees Ahmad

Subjects
SUGAR, ALLELES, GENOTYPES, SUGARCANE growing, SUGARCANE, GENES, ALTITUDES
Abstract

Copyright of Genetika (0534-0012) is the property of Serbian Genetics Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2019
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42. Molecular characterization, cloning and sequencing of coat protein gene of a Pakistani potato leaf roll virus isolate and its phylogenetic analysis

Author

Hossain, Md Belal, Nasir, Idrees Ahmad, Tabassum, Bushra, and Husnain, Tayyab

Subjects
Potato, potato leaf roll virus (PLRV), coat protein (CP) gene, reverse transcriptase polymerase chain reaction (RT-PCR), PLRV-CP Pakistani isolate
Abstract

Total RNA was extracted from potato leaf roll virus (PLRV) positive potato plants and complementary DNA were synthesized. Reverse transcriptase polymerase chain reaction (RT-PCR) based detection conditions were optimized by using coat protein (CP) gene specific primers. A 346 bp amplicon of PLRV- coat protein (CP) gene was amplified. Amplified CP-gene of PLRV was cloned in TA cloning vector, PCR ® 2.1. The clone was confirmed subsequently through restriction digestion analysis. PCR amplification used cDNA clone as a template and nucleotide sequencing. Expected nucleotide sequence of amplified PLRV-CP gene show homology of 94 to 97% when compared to the sequences already reported in GenBank database. This explored novel PLRV-CP gene was submitted at NCBI GenBank for characterization of PLRV Pakistani isolate (Accession No. JN039286). Phylogenetic analysis was also carried out and tree was made by using MEGA 4.0.Key words: Potato, potato leaf roll virus (PLRV), coat protein (CP) gene, reverse transcriptase polymerase chain reaction (RT-PCR), PLRV-CP Pakistani isolate.

Published
2016

43. Viability assessment of in vitro produced synthetic seeds of cucumber

Author

Tabassum, Bushra, Nasir, Idrees Ahmad, Farooq, Abdul Munim, Rehman, Ziaur, Latif, Zakia, and Husnain, Tayyab

Subjects
Artificial seed, Cucumis sativus, encapsulation, somatic embryogenesis, sodium-calcium alginate
Abstract

Friable, embryogenic calli of F1 cucumber (Cucumis sativus) cultivar, Royal, were induced from the hypocotyl pieces cultured on solidified MS-basal media supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) and benzyl amino purine (BAP). Embryogenic calli were transferred to liquid Murashige and Skoog (MS)-basal media supplemented with 5 ƒÊM naphthaleneacetic acid (NAA) and 1 µM BAP. The mature somatic embryos were encapsulated in sodium alginate mixture in synthetic seeds. The encapsulation mixture containing 3% sodium alginate, 100 mM calcium chloride and one-fourth volume of the cell suspension nutrient mixture containing 5x10-4 somatic embryos per ml was found the best. Synthetic seeds remain viable up to 14 weeks when stored at 4°C. Germination efficiency of synthetic seeds was decreased to 57% after 10 weeks of storage followed by rapid decrease in survival rate to 0% after 15 weeks. Genetic diversity between mother plants and in vitro produced synthetic seeds showed resemblance as assessed by amplified fragment length polymorphism (AFLP) markers.Key words: Artificial seed, Cucumis sativus, encapsulation, somatic embryogenesis, sodium-calcium alginate.

Published
2016

50. Biochemical and molecular characterization of catalase enzyme in the saprobic fungus: Sordaria fimicola.

Author

Ishfaq, Muhammad, Mahmood, Nasir, Akbar, Muhammad, Nasir, Idrees Ahmad, and Saleem, Muhammad

Abstract

Fungi have been used in modern scientific research due to their high potential for different enzymes production based on genomic features. The great proportion of soil mycoflora represented by saprobic fungi plays an important role in decomposition, thus contribute to the global carbon cycle. Sordaria fimicola strains (n= 61) collected from different environments were evaluated for catalase enzyme activity at first stage. Among all 61 isolates of S. fimicola, five strains viz. S1, S2, N7, N6 and SF13 were found to be most efficient in catalase enzyme activity. The complete catalase gene including exons and introns was amplified and sequenced from the most efficient strains of S. fimicola and then submitted in the NCBI data base under accession numbers KM282183, KM282184, KM282186, KM282185 and KM282182 for strains S1, S2, N7, N6 and SF13 respectively. The significant differences in the genes sequences and theoretically translated proteins were observed for all five strains of S. fimicola. As regards catalase enzyme activity, S. fimicola strains were found comparable to the Aspergillus niger strains, therefore being a saprophytic fungus with short life cycle S. fimicola can become a fungus of choice to produce catalase enzyme at large scale. [ABSTRACT FROM AUTHOR]

Published
2019

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