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Although fumonisins are identified as responsible for alterations in weight gain, little information is available on their effects on expression of growth-related genes, especially for Nile tilapia (Oreochromis niloticus) fingerlings. In this study, Nile tilapia fingerlings were treated with increasing levels of fumonisin B1 (FB1) and fumonisin B2 (FB2) (diets of 0, 20, 40, and 60 mg/kg) to evaluate their effects on weight gain (WG), feed intake (FI), feed efficiency (FE), growth hormone receptor (GHR) and insulin growth factor 1 (IGF-1) mRNA expression in liver of this fish. All variables were evaluated at 15 and 30 days of treatment. Diet containing 0 mg fumonisin/kg was used as control treatment. Treatment with 20, 40, and 60 mg fumonisin/kg of diet significantly reduced WG (P1 + FB2) affect the growth of Nile tilapia fingerlings through mechanisms that involve reduction of GHR and IGF-1 expression.

The aim of this study was to evaluate the influence of the subcutaneous fat thickness (SFT) on the regional composition of the carcass, the tissue composition of cuts, and the leg muscularity index of Santa Inês lambs. This experiment involved 24 uncastrated male lambs at approximately 100 days of age and at an average live weight of 22.70 ± 3.75 kg that were kept in the feedlot, where they received a complete pelleted diet formulated to provide a daily gain of 0.30 kg. The animals were slaughtered as they reached the pre-defined SFT of 2.00, 3.00, and 4.00 mm, evaluated by ultrasonography. Lambs slaughtered with 4.00 mm SFT had a heavier shoulder (1.80 kg) and leg (2.99 kg), differing from those slaughtered with 2.00 mm. No significant differences were observed (P > 0.05) between the treatments for the percentages of muscle in the cuts, which averaged 48.38% for the neck, 58.71% for the shoulder, 43.87% for the ribs, 53.56% for the loin, and 64.52% for the leg. Lambs slaughtered with 4.00 and 3.00 mm SFT differed from those slaughtered with 2.00 mm SFT for the percentage of total fat in the shoulder, which averaged 20.10, 19.02, and 15.79%, respectively. The animals slaughtered with 2.00 mm of fat exhibited the highest percentage of bone in the loin (20.23%). Leg muscularity was lower (0.34) in those slaughtered with 2.00 mm of subcutaneous fat. Slaughtering Santa Inês lambs with different subcutaneous fat thicknesses yields different regional compositions of the carcass, tissue compositions of cuts, and leg muscularity indices. It is recommended to slaughter Santa Inês lambs when they reach a subcutaneous fat thickness of 3.00 mm.

O efeito da maturação nas características físicas (pH, cor, perda de peso na cozedura e força de cisalhamento) e biológico (microbiologia, no que se refere aos mesófilos e psicrotrófilos e comprimento do sarcômero) do músculo Longissimus thoracic lumborum do gado Nelore adulto foi avaliado durante quatro períodos de maturação (0, 7, 14 e 21 dias) e duas espessuras de corte (2,5 e 7,5 cm). Foram adquiridos 64 músculos de Longissimus thoracic lumborum de machos castrados Nelore do Frigorífico Nutrial, Sergipe, Brasil. O estudo foi realizado utilizando um delineamento inteiramente casualizado, correspondente a um fatorial de 2 x 4 com oito repetições. A análise microbiológica revelou as condições sanitárias em que as amostras foram manipuladas e armazenadas, produzindo contagens de psicrotrófilos (2,18 x 105 UFC g-1) e mesófilos (2,4 x 105 UFC g-1) bem abaixo do nível crítico de deterioração (106 UFC g-1). O pH (5,57) e a perda no cozimento (24,13%) não foram influenciados pelas variáveis avaliadas (P > 0,05). Os outros parâmetros avaliados variaram significativamente em função do período de maturação e da espessura do corte (ambos P < 0,05). A luminosidade da carne variou tanto com a espessura do corte quanto com o período de maturação. O corte de 2,5 cm de espessura maturado durante 14 dias e o corte de 7,5 cm de espessura maturado por 21 dias foi mais escuro do que o corte de 2.5 cm de espessura maturado por 7 dias e os 7.5- que eram as amostras de cores mais claras (semelhantes para carne não maturada). A intensidade da cor vermelha da carne foi diferente após 14 dias de maturação nos cortes de 7,5 cm (P < 0,05), mas as diferenças não foram evidentes em nenhum dos cortes de 2,5 cm (P > 0,05). Em relação à intensidade b*, o corte de 2,5 cm produziu uma coloração mais escura no tempo zero, que diminuiu com o período de maturação. No entanto, o corte de 7,5 cm diferiu entre as amostras não maturadas e as maturadas. Em relação à cor vermelha e amarela da gordura da carne (a* e b*, respectivamente), a* intensidade mais leve aos 7 dias de maturação ou corte de 2,5 cm (P < 0,05), enquanto a intensidade de b* foi semelhante a 7 dias de maturação em ambos os cortes (P > 0,05). A força de cisalhamento da carne diminuiu para 4,49 kgf aos 21 dias de maturação. O comprimento do sarcômero variou para o corte de 2,5 cm (P < 0,05), mas não para o corte de 7,5 cm, que permaneceu constante (P > 0,05). O músculo Longissimus thoracic lumborum de Nelore adulto deve ser maturado por no mínimo 14 dias, à vácuo, para resultar numa carne com qualidade aprimorada. The effect of maturation on the physical (pH, color, weight loss on cooking, and shear force) and biological (microbiology, concerning mesophiles and psychrotrophiles, and sarcomere length) characteristics of the Longissimus thoracic lumborum muscle of adult Nellore cattle was assessed over four maturation periods (0, 7, 14, and 21 days) and two cut thicknesses (2.5 and 7.5 cm). Sixty-four Longissimus thoracic lumborum muscles from castrated Nellore males were purchased from Frigorífico Nutrial, Sergipe, Brazil. The study was performed using a completely randomized design, corresponding to a 2 x 4 factorial with eight replicates. The microbiological analysis revealed the sanitary conditions in which the samples were handled and stored, yielding counts of psychrotrophiles (2.18 x 105 CFU g-1) and mesophiles (2.4 x 105 CFU g-1) well below the critical deterioration level (106 CFU g-1). The pH (5.57) and the loss on cooking (24.13%) were not influenced by the evaluated variables (P > 0.05). The other evaluated parameters significantly varied as a function of the maturation period and the cut thickness (both P < 0.05). Meat luminosity varied with both the cut thickness and maturation period. The 2.5-cm-thick cut matured for 14 days and the 7.5-cm-thick cut matured for 21 days were darker than the 2.5-cm-thick cut matured for 7 days and the 7.5-cm-thick cut matured for 14 days, which were the lighter-colored samples (similar to non-matured meat). The intensity of the red color of the meat was different beginning after 14 days of maturation in the 7.5-cm cuts (P < 0.05) but differences were not evident in any of the 2.5-cm cuts (P > 0.05). Regarding the b* intensity, the 2.5-cm cut yielded a darker staining at time zero, which decreased with the maturation period. However, the 7.5-cm cut differed between the non-matured and the matured samples. Regarding the red and yellow color of the meat fat (a* and b*, respectively), a* displayed lighter intensity at 7 days of maturation or the 2.5-cm cut (P < 0.05), while the intensity of b* was similar from 7 days of maturation onwards for both cuts (P > 0.05). The shear force of the meat decreased to 4.49 kgf at 21 days of maturation. The sarcomere length varied for the 2.5-cm cut (P < 0.05) but not for the 7.5-cm cut, which remained constant (P > 0.05). Longissimus thoracic lumborum muscle of adult Nellore cattle should be matured as a larger, vacuumpacked cut for a minimum of 14 days to produce meat with improved quality. Londrina, PR

Physical (pH, color, tenderness, and cooking weight loss), chemical (moisture, crude protein, ash, and lipids) and sensory (odor, flavor, juiciness, and overall acceptance) characteristics of meat from Santa Inês lambs slaughtered with 2.0, 3.0, and 4.0 mm of subcutaneous fat thickness (SFT) were evaluated. Samples of the longissimus dorsi muscles were removed from 24 male uncastrated lambs distributed in a completely randomized design with three treatments and eight replicates. Weightings and ultrasound evaluations were performed every 14 days. No significant differences were observed for pH0h, which averaged 6.24. Lambs slaughtered with 4.0 mm SFT differed from the treatments with 2.0 and 3.0 mm as to the pH24h values. The meat from lambs slaughtered with 3.0 and 4.0 mm SFT showed a greater lightness (L*) value. Meat tenderness (2.21 kgf/cm2), cooking weight loss (13.90%), moisture (74.39%), and crude protein (22.10%) contents were not affected by the SFT. For the total lipid contents of the meat, significant differences were observed between the lambs slaughtered with 2.0 mm and those with 3.0 and 4.0 mm SFT. The sensory traits were not affected by the SFT with which the lambs were slaughtered. Lambs slaughtered with 4.0 mm SFT remained 33 days longer in feedlot and showed similar live weights at slaughter to those with 3.00 mm. It is recommended to slaughter Santa Inês lambs with a subcutaneous fat thickness of 3.00 mm.

The objective of this work was to evaluate the allometric growth, the correlation of the cuts with the half carcasses, and the muscularity index of the leg of ½ Dorper + ½ Santa Inês lambs slaughtered with 2.0, 3.0, and 4.0 mm of subcutaneous fat thickness (SFT). Twenty-four non-castrated male lambs were used, being distributed according to their weight into three collective pens. Slaughtering occurred as the lambs reached the SFT predetermined by ultrasound. The half carcasses were weighed, dissected, and separated into five cuts: neck, shoulders, rib, loin, and leg. These cuts were dissected and weighed into muscle, subcutaneous fat, intermuscular fat, and bone. In the shoulders, lambs with 2.0 and 4.0 mm SFT showed early growth. In the rib, this early precocity was observed in lambs with 4.0 mm SFT. The leg showed isogonic growth in lambs with 3.0 and 4.0 mm SFT and was the cut that best correlated with the half carcass, regardless of the SFT. The slaughter of ½ Dorper + ½ Santa Inês lambs with 4.0 mm of subcutaneous fat allows obtaining a better allometric growth of the shoulder, rib, and leg cuts, as well as a better leg muscularity index. O objetivo deste trabalho foi avaliar o crescimento alométrico, a correlação dos cortes com as meia-carcaças e o índice de musculosidade da perna de cordeiros ½ Dorper + ½ Santa Inês abatidos com 2,0, 3,0 e 4,0 mm de espessura de gordura subcutânea (EGS). Utilizaram-se 24 cordeiros machos, não castrados, distribuídos de acordo com seu peso, em três baias coletivas. Os abates ocorreram à medida que os cordeiros atingiram a EGS pré-determinada por ultrassonografia. As meia-carcaças foram pesadas, dissecadas e separadas em cinco cortes: pescoço, paleta, costilhar, lombo e perna. Estes cortes foram dissecados e pesados em músculo, gordura subcutânea, gordura intermuscular e osso. Na paleta, cordeiros com 2,0 e 4,0 mm de EGS mostraram crescimento precoce. No costilhar, observou-se essa precocidade em cordeiros com 4,0 mm de EGS. A perna mostrou crescimento isogônico em cordeiros com 3,0 e 4,0 mm de EGS e foi o corte que melhor correlacionou-se com a meia-carcaça, independentemente da EGS. O abate de cordeiros ½ Dorper + ½ Santa Inês com 4,0 mm de espessura de gordura subcutânea permite obter melhor crescimento alométrico dos cortes paleta, costilhar e perna, bem como melhor índice de musculosidade da perna. To Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes), for partial financing (Finance Code 001); to Programa de Estímulo à Mobilidade e ao Aumento da Cooperação Acadêmica da Pós- Graduação em Sergipe (Promob), for support (Capes/ Fapitec/SE numbers 08/2013 and 10/2016); and to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), for financial support. info:eu-repo/semantics/publishedVersion

Foram utilizadas 24 cordeiras do grupo genético Pantaneiro, abatidas com 2,0; 3,0 e 4,0 mm de espessura de gordura, avaliadas por ultrassonografia no Longissimus dorsi. Avaliaram-se características quantitativas de carcaça, do lombo e índice de musculosidade da perna. As cordeiras receberam ração completa misturada calculada para ganho de peso diário de 0,300 kg. Foram realizadas avaliações por ultrassonografia (entre a 12ª e a 13ª costela) e pesagens a cada 14 dias, sendo que os abates ocorriam à medida que as cordeiras atingiam a espessura de gordura pré-determinada: 2,0; 3,0 e 4,0mm. Não houve efeito para rendimento verdadeiro da carcaça, rendimento comercial da carcaça e índice de compacidade da perna. Foi observado maior rendimento do lombo para cordeiras abatidas com 4,0mm de espessura de gordura subcutânea. As espessuras de gordura subcutânea não influenciaram a área de olho de lombo, porém animais com 4,0 mm apresentaram maiores medidas. Foram observadas diferenças nas proporções de músculo, osso e gordura da perna entre as espessuras de gordura subcutânea avaliadas. A musculosidade da perna não foi influenciada com o aumento da espessura da gordura subcutânea. Com base neste estudo e também com as exigências do mercado consumidor, recomenda-se o abate de animais com aproximadamente 3,00 mm de gordura. Twenty-four female lambs, all from the Pantaneiro genetic group, slaughtered with 2.0; 3.0 and 4.0 mm of subcutaneous fat thickness measured by ultrasound in Longissimus dorsi, were used. We evaluated quantitative carcass traits, loin and leg muscularity. The lambs were fed with total ration calculated for daily weight gain of 0.3 kg. Ultrasound evaluations (between the 12th and the 13thribs) and weightings were carried out each 14 days; the slaughter occurred as the lambs reached the predetermined fat thickness: 2.0, 3.0 and 4.0 mm regardless of body weight. There was no effect for real income carcass, commercial carcass yield and index of leg compactness. Higher yield of loin for lambs slaughtered at 4.0 mm fat thickness was observed. The thickness of subcutaneous fat did not affect loin eye area, but animals with 4.0 mm had higher measures. There were differences in the proportions of muscle, bone and fat leg between subcutaneous fat thicknesses measured. The leg musculature was not influenced by increasing the thickness of subcutaneous fat. Based on this study and also with the requirements of the consumer market, it is recommended that the slaughter of animals occur with approximately 3.00 mm fat.

A microbiologia do rumen e complexa e ampla devido ao grande numero de organismos nela presente, modificacoes na dieta promovem mudancas no ambiente e consequentemente na microbiota ali presente. Determinacoes da concentracao e composicao da fauna em ruminantes sobre diversos tipos de alimentacao, sao importantes para a elucidacao da presenca dos protozoarios no rumen e suas relacoes com o hospedeiro. Nesta revisao de literatura sao analisados os efeitos da defaunacao do ambiente ruminal, e as possiveis respostas produtivas aos ruminantes. Palavras-chave: digestao, defaunacao, microbiota

Made available in DSpace on 2018-11-12T17:26:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-09-01. Added 1 bitstream(s) on 2021-07-14T17:51:18Z : No. of bitstreams: 1 S1519-99402015000300712.pdf: 178824 bytes, checksum: a696d90e7d33f822d6a1399f5afabc54 (MD5) ResumoObjetivou-se avaliar as características quantitativas das carcaças de cordeiros da raça Santa Inês em confinamento, abatidos com três espessuras de gordura subcutâneas (EGS) diferentes. Foram utilizados 24 cordeiros, machos não castrados da raça Santa Inês, com aproximadamente 100 dias de idade e peso vivo de 22,7±3,75kg, divididos aleatoriamente em três tratamentos: abates com espessura de gordura subcutânea de 2,0; 3,0 e 4,0mm. O delineamento experimental adotado foi o inteiramente casualizado, com três tratamentos e oito repetições por tratamento. Para as análises estatísticas utilizou-se o softwareSAEG Os cordeiros abatidos com 2,0mm apresentaram maiores rendimentos do fígado (2,12%), baço (0,32%), cabeça (6,69%) e patas (3,08%), havendo um declínio nos rendimentos com o aumento da EGS. Os rendimentos de carcaça na origem, no frigorifico, comercial e verdadeiro, sofreram interferência das EGS (p

Objetivou-se avaliar os parâmetros fisiológicos e o desempenho de ovelhas da raça Santa Inês e cordeiros ½ Dorper - Santa Inês nas estações verão e inverno. Foram utilizadas 160 ovelhas da raça Santa Inês e 189 cordeiros ½ Dorper - Santa Inês em delineamento inteiramente casualizado distribuídas em dois tratamentos, verão: 80 ovelhas e 92 cordeiros e no inverno: 80 ovelhas e 97 cordeiros. Registrou-se os parâmetros ambientais, temperatura do ar (TaoC), umidade relativa (UR%), velocidade do vento (Vv m.s-1) e temperatura do globo negro (TgoC) e parâmetros fisiológicos, temperatura retal (TRoC), frequência respiratória (FR mov.min-1) e frequência cardíaca (FC bat.min-1). A condição corporal das ovelhas foi aferida ao parto. No terço final de gestação verificou-se efeito significativo (PoC, 102,58 (mov.min-1) e 128,34 (bat.min-1) com ICT 46, e no inverno foi de 38,34oC, 35,71 (mov.min-1), 77,96( bat.min-1) com ICT de 14. Os valores de TR, FR e FC ao parto e ao desmame também foram significativas entre as estações verão e inverno. Foram verificadas diferenças (P This study aimed to evaluate the physiological parameters and performance of sheep Santa Inês and Dorperlambs ½-SantaInes in summer and winter seasons. 160 Santa Inês sheep and 189 ½ Dorper lambs were used - Santa Inês completely randomized in two treatments, summer: 80 sheep and 92 lambs and winter: 80 sheep and 97 lambs.Was recorded environmental the environmental parameters of air temperature (Ta°C), relative humidity (% RH), wind speed (Vvms- 1) and black globe temperature (Tg°C) and physiological parameters of rectal temperature (Tr°C), respiratory rate (RR mov.min-1) and heart rate (HR beat.min - 1). The body condition of the ewes was measured at birth. In the final third of gestation there was a significant effect P < 0.05) for TR, FR and HR on sheep, where in summer the values were 40.63°C, 102.58 (mov.min-1) and 128,34 (beat.min-1) with 46 ICT, and winter was 38.34°C , 35.71 (mov.min-1), 77.96 (beat.min-1) with ICT of 14. The values of TR, RR and HR at birth and weaning were also significant between the summer and winter seasons. Differences were observed (P < 0.05) for TR, RR and HR of lambs at birth and weaning between summer and winter. The weight at birth (4.51 ± 2.86) and weaning (16.91 ± 3.91) were higher for those born in the summer. During the summer Santa Inês ewes were superior in body condition score, favoring births of heavier lambs and consequently higher weaning weight with higher rate of survival than those found in winter.

Após a sincronização de estro e cobertura, 120 fêmeas Santa Inês foram distribuídas em quatro tratamentos de Nutrientes Digestíveis Totais (NDT): 59,68%; 66,28%; 72,93%; e 79,39%, utilizando-se ração total, de 0 a 50 dias de gestação, para avaliar o índice de prenhez em função dos níveis energéticos da dieta e da taxa de glicose circulante, bem como a absorção embrionária em função da condição corporal (CC) e da taxa de glicose circulante no momento da cobertura. No dia da cobertura e a cada dez dias, foram coletados 5 mL de sangue para determinação imediata do teor de glicose e para dosar a progesterona circulante. A partir do 25º dia pós-cobertura, realizaram-se exames de ultrassonografia, para acompanhamento da gestação, visando avaliar perdas embrionárias. A taxa de perda embrionária foi correlacionada com os níveis energéticos das dietas e com os níveis de glicose circulante e, antes dos 20 dias, utilizaram-se os níveis sanguíneos de progesterona para estimar a prenhez. As fêmeas que apresentaram valores de progesterona acima de 1 ng/mL de plasma foram consideradas prenhas aos 20 dias. A mortalidade embrionária não foi influenciada (P˃0,05) pela taxa de glicose circulante das ovelhas, no dia da cobertura. A taxa de prenhez foi influenciada (P

Twenty-four female lambs of the Pantaneiro genetic group, with approximately 100 days of age, average body of 16.24 ± 1.78 kg, were slaughtered with 2.0, 3.0 and 4.0 mm of subcutaneous fat thickness, measured by ultrasound in the Longissimus, to evaluate the allometric growth. Lambs fed a complete pelleted diet calculated to ensure a daily weight gain of 0.30 kg. Slaughtering was performed as the female lambs reached the pre-established fat thickness. After 24 hours in a cold chamber at 4ºC, chilled carcasses were cut in half and weighed. The right side was separated into five sections to determine the allometry of carcasses and cuts. Neck and rib showed isogonic growth in all treatments. Loin has remained late growth to 2.0 and 3.0 mm. Shoulder and leg were isogonic growth in the treatments 3.0 and 4.0 mm. The leg in relation to half carcass showed the greatest correlation. The cuts exhibited differentiated development in the growth rates of tissues. It is recommended to slaughter female lambs when they reach 3.0 mm of subcutaneous fat thickness, since the carcass has already reached physiological maturity. Shoulder and leg cuts are best suited for dissection for presenting the highest correlation with the carcass in females of the Pantaneiro group. Foram utilizadas 24 cordeiras do grupo genético Pantaneiro, com aproximadamente 100 dias de idade e peso corporal médio de 16,24 ± 1,78 kg, abatidas com 2,0; 3,0 e 4,0 mm de espessura de gordura subcutânea, avaliadas por ultrassonografia sobre o Longissimus, para avaliar o crescimento alométrico. As cordeiras receberam ração completa peletizada para ganho de peso diário de 0,30 kg. Os abates ocorreram à medida que as cordeiras atingiam a espessura de gordura pré-determinada. Após 24 horas refrigeradas a 4ºC, as carcaças frias foram seccionadas ao meio e pesadas. O lado direito foi separado em cinco cortes para determinação da alometria da carcaça e dos cortes. Nos cortes pescoço e costilhar, os tratamentos apresentaram crescimento isogônico. O lombo manteve-se tardio para 2,0 e 3,0 mm. A paleta e perna apresentaram crescimento isogônico nos tratamentos 3,0 e 4,0 mm. A perna em relação à meia carcaça apresentou a maior correlação. Os cortes obtiveram desenvolvimento diferenciado nas taxas de crescimento dos tecidos. Recomenda-se o abate de cordeiras Pantaneiras com 3,0 mm de espessura de gordura pela carcaça já ter atingido a sua maturidade fisiológica. Os cortes paleta e perna são os mais indicados para dissecação por apresentarem a maior correlação com a carcaça, em fêmeas do grupo Pantaneiro.

Foram utilizados trinta e quatro cordeiros machos não castrados, sendo 16 Santa Inês (SI) e 18 ½ Dorper + ½ Santa Inês (½ D+ ½ SI). Os animais foram abatidos com 2,0; 3,0 e 4,0mm de espessuras de gordura subcutânea, para verificar o efeito do grupo genético e espessura de gordura ao abate na composição química da carne de cordeiros. Os cordeiros foram confinados, recebendo ração completa peletizada, calculada para ganho médio diário de 0,30kg. Foram realizadas avaliações por ultrassonografia na região do lombo entre a 12ª e 13ª costelas. Para os teores de lipídeos totais da carne foram observadas diferenças entre o grupo genético (SI: 4,78g/100g; ½ D+ ½ SI: 6,45g/100g) e as espessuras de gordura ao abate (2,0mm: 4,77g/100g; 3,0mm: 5,20g/100g e 4,0mm: 6,86g/100g). Foram observadas diferenças para o teor de ácidos graxos saturados nas diferentes espessuras ao abate (2,0mm: 26,52g/100g; 3,0mm: 27,07g/100g e 4,0mm: 24,63g/100g). Constatou-se a presença de um isômero de ácido linoleico conjugado, C18:1n 9cis ,11trans. Analisando-se a concentração total dos ácidos graxos insaturados, observou-se que diferentes espessuras de gordura influenciaram significativamente as concentrações dos ácidos graxos poliinsaturados (2,0mm: 8,36g/100g; 3,0mm: 8,54g/100g e 4,0mm:11,18g/100g). A carne de cordeiros demonstrou ser altamente benéfica no teor lipídico e de um isômero de Ácido Linoleico Conjugado. Nas condições de realização do experimento a carne de cordeiros com 4,0mm de espessura de gordura subcutânea demonstraram melhor incremento de ácidos graxos poli-insaturados em relação aos demais, melhorando assim a qualidade nutricional da carne tornando-a mais adequada para a alimentação humana. Thirty-four not castrated lambs, 16 Santa Ines (SI) and 18 ½ Dorper + ½ Santa Ines (½ D + ½ SI), were slaughtered with different subcutaneous fat thicknesses to verify the effect of genotype and fat thickness on chemical composition of the meat. The lambs remained confined, from weaning to slaughter, and they were fed with complete pelleted ration calculated for daily weight gain of 0.3 kg. Ultrasound evaluations were performed between the 12th and 13th ribs in the loin area. The slaughter was carried out when the lambs reached the thickness of fat pre-determined: 2.0, 3.0 and 4.0 mm. There were differences in the total lipids of the meat between the genetic groups (SI: 4.78g / 100g; ½ D ½ + SI: 6.45g / 100g) and fat thickness at slaughter (2.0mm: 4.77g / 100g; 3.0mm: 5.20g / 100g and 4.0mm: 6.86g / 100g). The contents of fatty acids differed in the different fat thicknesses at slaughter (2.0mm: 26.52g/100g; 3.0mm: 27.07g/100g e 4.0mm: 24.63g/100g). It was observed the presence of an isomer of Conjugated Linoleic Acid, C18: 1n 9cis, 11trans. Analyzing the total concentration of unsaturated fatty acids, it was observed that the different thicknesses of fat signifycantly affected the concentration of polyunsaturated fatty acids (2.0mm: 8.36g/100g; 3.0mm: 8.54g/100g e 4.0mm: 11.18g/100g). Meat from lambs proved highly beneficial in lipid content and an isomer of CLA. Under the conditions of the experiment the lamb meat slaughtered with 4.0mm thickness of subcutaneous fat showed better increase of polyunsaturated fatty acids in relation to the other, thus improving the nutritional quality of the meat making it more suitable for human consumption.

The accuracy of the FAMACHA© method was evaluated on the identification of female sheep fed two levels of crude protein, naturally infected with Haemonchus contortus, by means of the correspondent hematocrit value. Forty-seven female sheep of the breeds Santa Inês (n = 16), Texel (n = 16) and Ile de France (n = 15) aged between eight and twelve months were assigned to two treatments, 12 or 16 % crude protein in the diet. All the animals were wormed thirty days before the first data collections, which were done fortnightly between July 2005 and March 2006. The color of the ocular conjunctiva was individually evaluated according to the precepts of the FAMACHA© method and the hematocrit value of each animal was obtained in laboratory. A correlation of 1:0.7991 was found between the hematocrit values and the classification given by the FAMACHA© method aiming to identify animals with different degrees of anemia. The method was efficient to identify animals to worm, thus representing a support in the identification of animals susceptible to Haemonchus contortus. Avaliou-se a precisão do método FAMACHA© na identificação de fêmeas ovinas alimentadas com dois níveis de proteína bruta, infectadas naturalmente por Haemonchus contortus por meio do valor de hematócrito correspondente. Foram utilizadas 47 fêmeas ovinas das raças Santa Inês (n = 16), Texel (n = 16) e Ile de France (n = 15), com idades entre oito e 12 meses. Dentro de cada raça, os animais foram distribuídos em dois tratamentos, 12 ou 16% de proteína bruta total na dieta. Todos os animais foram vermifugados 30 dias antes das primeiras coletas de dados que foram feitas quinzenalmente, no período entre julho de 2005 e março de 2006. Avaliou-se individualmente a mucosa ocular de todos os animais em concordância com o preceituado pelo método FAMACHA©, bem como se obteve o valor do hematócrito de cada animal avaliado em laboratório. Obteve-se uma correlação de 1:0,7991 dos valores de hematócrito e a classificação pelo método FAMACHA© visando a identificação de animais acometidos por diferentes graus de anemia. O método mostrou-se eficiente na identificação dos animais que necessitam de tratamento antiparasitário constituindo-se como suporte na identificação de animais susceptíveis ao Haemonchus contortus.

The aim of this experiment was to evaluate the productive and economic performance and quantitative traits of crossbreds half Dorper half Santa Ines (½ D-SI) lambs carcasses and purebreds Santa Ines (SI) slaughtered with different subcutaneous fat thicknesses. Thirty-four lambs were used, sixteen were SI and eighteen were ½ D-SI. The lambs were kept confined and received a diet to provide an average daily weight gain of 0.300 kg. Weekly weightings and admeasurements of subcutaneous fat thicknesses through ultrasonography (between the 12a and 13a ribs) were made, and the lambs were killed as the predetermined fat thicknesses (2.0; 3.0 and 4.0 mm) were reached. There wasn’t breed effect on the productive performance. Concerning to subcutaneous fat thicknesses, it was noticed an advantage on the animals final weight when these were killed with 4.0 mm. This advantage was of 7.86 and 3.81 kg more compared to those animals that were killed with 2.0 and 3.0 mm respectively. There was differences on food ingestion between all the fat thicknesses. Furthermore, the lambs killed with subcutaneous fat thicknesses of 2.0 and 3.0 mm had a better feed conversion rate (FCR) when compared to the ones killed with 4.0 mm. The economical evaluation expressed in absolute values provided an increased net income as the subcutaneous fat thickness would increase. The commercial and biological returns were affected by fat thicknesses at slaughter and both variables were bigger on thicknesses of 4.0 mm followed by 3.0 and finally 2.0 mm. About meat cuts, the loin yield of crossed animals was superior to Santa Ines ones, possibly due to the genetic makeup in which the crossbred animals have 50% of a genotype skilled for meat production. There wasn’t any breed difference for loin eye area (LEA), Measure C, FTU, and Measure J. However, for all variables, a higher value for animals killed with fat thickness of 4.0 mm was observed, followed by the ones of 3.0 mm and finally 2.0 mm.

Linseed plays an important role in animal nutrition as it contains long-chain fatty acids in its composition, which, once absorbed, are incorporated into meat and milk. For evaluate the concentration of fatty acids in the Longissimus dorsi muscle of culling ewes fed diets containing levels of inclusion of linseed, 88 culling ewes were used with an average initial body weight of 37.65 ± 6.98 kg were distributed in a completely randomized design with 12 treatments. Treatments consisted of the interaction between levels of linseed (0, 5, 10, and 15%) and days in feedlot (30, 45, and 60). The most present fatty acids in the composition of the muscle L. dorsi were palmitic (27.32 g 100 g-1) and stearic (17.77 g 100 g-1). Saturated acids remained at low levels as the animals were fed greater levels of linseed, demonstrating the importance of introducing quality foods in animal feeding. Oleic acid was the most present monounsaturated fatty acid, with 40 g 100 g-1. Palmitoleic and elaidic fatty acids increased linearly as the linseed inclusion in the diet was increased. Polyunsaturated acids increased with the presence of linseed in the diet. For the linolenic acid (C18: 3n3), the best result was with inclusion of 10% of linseed in the total diet. All groups of animals that received linseed obtained better n6:n3 ratios, which varied from 1.81 to 4.14. The higher CLA values obtained in this study are related to the higher amounts of inclusion of linseed in the sheep diet, varying from 1.15 to 5.72 g 100 g-1. It is recommended culling ewes supplemented with inclusion of 10% linseed, for 60 days in feedlot, because they comprise a larger number of favorable traits regarding to the profile fatty acids of Longissimus dorsi.