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1. BODIPY-based dye for no-wash live-cell staining and imaging

Author

Alexey A. Pakhomov, Igor E. Deyev, Natalia M. Ratnikova, Stepan P. Chumakov, Veronika B. Mironiuk, Yuriy N. Kononevich, Aziz M. Muzafarov, and Vladimir I. Martynov

Subjects
fluorophore, aggregation-caused quenching, BODIPY, live-cell imaging, flow cytometry, fluorescence microscopy, Biology (General), QH301-705.5
Abstract

In nonpolar solvents, hydrophobic organic fluorophores often show bright fluorescence, whereas in polar media, they usually suffer from aggregation-caused quenching (ACQ). Here, we harnessed this solvatochromic behavior of a 1,3,5,7-tetramethyl-BODIPY derivative for cell staining and applied it to live-cell imaging and flow cytometry. As opposed to commercially available dyes, this BODIPY derivative showed excellent contrast immediately after staining and did not require any wash-off.

Published
2017
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2. BODIPY-based dye for no-wash live-cell staining and imaging

Author

Veronika B. Mironiuk, Vladimir I. Martynov, Yuriy N. Kononevich, Stepan P. Chumakov, I. E. Deyev, Aziz M. Muzafarov, Natalia M. Ratnikova, and Alexey A. Pakhomov

Subjects
Boron Compounds, Fluorophore, Cell Survival, 010402 general chemistry, Photochemistry, 01 natural sciences, Methylation, General Biochemistry, Genetics and Molecular Biology, chemistry.chemical_compound, Live cell imaging, Fluorescence microscope, Humans, Fluorescent Dyes, Quenching (fluorescence), Staining and Labeling, 010405 organic chemistry, Chemistry, Solvatochromism, Optical Imaging, Flow Cytometry, Fluorescence, 0104 chemical sciences, Staining, Microscopy, Fluorescence, BODIPY, Biotechnology, HeLa Cells
Abstract

In nonpolar solvents, hydrophobic organic fluorophores often show bright fluorescence, whereas in polar media, they usually suffer from aggregation-caused quenching (ACQ). Here, we harnessed this solvatochromic behavior of a 1,3,5,7-tetramethyl-BODIPY derivative for cell staining and applied it to live-cell imaging and flow cytometry. As opposed to commercially available dyes, this BODIPY derivative showed excellent contrast immediately after staining and did not require any wash-off.

Published
2017

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