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El presente estudio tuvo como objetivo evaluar las relaciones entre ideación suicida, riesgo suicida, resiliencia y autoestima en una muestra de 59 adolescentes indígenas zenúes del Resguardo de San Andrés de Sotavento, en el departamento de Córdoba (Colombia), con una edad media de 17 años, a quienes se aplicó la Escala de Autoestima de Rosenberg, el Inventario de Ideación Suicida Positiva y Negativa (PANSI), la Escala de Afrontamiento Breve Resiliente y la Escala para la Evaluación del Riesgo Suicida. Se encontraron niveles altos en el PANSI positivo y bajos en el negativo; medio altos en resiliencia; moderados en autoestima, y en riesgo suicida se observó una buena percepción al tener niveles bajos. Finalmente, el análisis de correlación mostró que la ideación suicida correlacionó negativamente con autoestima y la dimensión de riesgo suicida con autolesión; la resiliencia se asoció negativamente con riesgo suicida y, por último, la autoestima correlacionó negativamente con riesgo suicida. En conclusión, los niveles resiliencia y autoestima influyen directamente en el riesgo e ideación suicida en los jóvenes indígenas, hallándose una relación inversa entre estas variables.

The emergence of the Internet of Things (IoT) has the potential to reframe interactions among tourists, destinations, and service providers in multiple ways. Yet, there is scant empirical evidence on how individuals develop their relationships with IoT devices and how this technology can serve tourists in planning and deciding on particular destinations or services. Drawing on the assemblage theory, we investigated tourists’ interactive experiences when planning trips with and without smart speakers. Methodologically, we employed an interactionist/performative approach that included three qualitative studies to examine tourists’ information production, expressive roles, and information processing styles during interactions with smart speakers in the pre-visit stage. The analysis was driven by grounded theory and utilized computerized psycholinguistic techniques to enrich our research implications for theory, methodology, and tourism management.

PurposeAlthough value co-creation has been widely analyzed in digital contexts and various types of services (tourism, healthcare, etc.), it has received less study in the area of retail distribution. This study proposes that trust in the retailer and perceived support can encourage co-creation behavior on various levels: a basic level related to communication of service errors and a moderate-high level related to participation in service innovation. This study also proposes modeling for two different segments according to the participation in a loyalty program and according to the relationship duration.Design/methodology/approachA survey (N = 644) was used to test the model in the context of the consumer goods retail industry. Confirmatory factor analysis and multigroup structural equation modeling techniques were used to assess the proposed model.FindingsThe results show differences in the formation of co-creation behaviors depending on the customers analyzed. To encourage communication of service errors, customers affiliated to the program and customers with the longest customer-firm relationships must trust the distributor. Perceived support is crucial in encouraging feedback on service errors among non-affiliated and new customers. For promoting service innovation, the most significant antecedent is perceived support, followed by trust, independently of whether or not the customer belongs to the loyalty program. Customers with the longest relationship participate in co-innovation motivated equally by trust and perceived support. Customers with shorter relationship duration only participate in co-innovation if they perceived support.Originality/valueThis study contributes to deepening knowledge of co-creation behavior in the field of retail distribution. To date, research in this context has not considered the existence of various levels of co-creation: the basic level related to feedback on service errors and the moderate/high level related to participation in service co-innovation. Nor have studies tested the influence of trust and perceived support on these co-creation behaviors. Further, this study is the first study to integrate two significant variables that moderate retailers' strategy in the same model: membership in a loyalty program and duration of customer-firm relationship.

The Coronavirus 2019 (COVID-19) outbreak represents the first time that an international health crisis of this level has been experienced in the digital age. This research aims to determine the cau...

Purpose: The purpose of this paper is to analyse the impact of consumers’ perceptions of the enjoyment and originality of firm-generated content (FGC) posted on Instagram on affective customer engagement (CE). In addition, an examination is undertaken of affective CE as a driver of customer behaviour. Design/methodology/approach: The paper takes a quantitative approach using a sample of 334 women followers of an eco-friendly restaurant Instagram account. After validation of the measurement scales, the hypotheses were tested through structural equation modelling. Drawing on the stimuli-organism-response framework the authors posit that consumers’ perceptions of the enjoyment and originality of Instagram posts generate affective CE, which, in turn, influences customer behaviour. Findings: The results showed that the perceived enjoyment and perceived originality of Instagram posts generated by an eco-friendly restaurant have a positive influence on affective CE, which, in turn, affects consumers’ recommendation behaviours, intention to follow the restaurant’s advice on Instagram and intention to revisit the restaurant. Originality/value: This research provides novel insights into how the perceived enjoyment and originality of FGC posted on Instagram increases women’s affective engagement and expands knowledge of how affective CE might increase positive electronic word-of-mouth, intention to follow the restaurant’s advice and repurchase intentions., The authors gratefully acknowledge the financial support of the University of Valencia (Spain) under Grant UV-INV-AE19-1212255.

Value co-creation by users in a virtual community is a key element to encouraging the community’s brand equity. This study analyzes the effect of the functional value provided by the virtual community on the two value-co-creation behaviors that occur within it: (1) self-value co-creation and (2) communal value co-creation. Through self-value co-creation, participants co-create value to their own benefit by becoming involved in co-designing their experience. By communal value co-creation, participants co-create value through evaluations, recommendations, and ideas that benefit others. This study also asks whether multichannelity—using various channels to access the virtual community (website and mobile app), as opposed to using a single channel (website or mobile app)—has a moderating effect on the relationships proposed between value co-creation and brand equity of the virtual community. The analysis is contrasted empirically for the virtual community Tripadvisor with data collected by a research institute via telephone interview. Confirmatory factor analysis and multi-group structural equation modeling techniques were used to assess the proposed model. The study enriches two significant lines of scholarly research, value co-creation and brand equity. It does so in multi-brand virtual contexts in which variety of service brands coexists with the brand of the virtual community, and with users who access the virtual community through one or various channels. The study also contributes to the formulation of business strategies oriented to increasing the brand equity of virtual communities, Spanish Ministry of Science and Innovation, grant number PID2020-113561RB-I00 and benefited from the Professorship Excellence Program in accordance with the multi-year agreement signed by the Government of Madrid and the Autonomous University of Madrid

Purpose The evolution of private labels (PL) is a recent trend in the retail industry: many retailers now manage a PL portfolio that includes multiple value propositions, as well as various brand name strategies. Little research has been done, however, on how this combination of PL strategies conditions the results of the retailer that manages them. This study aims to examine the formation of PL brand equity and its effect on store loyalty for retailers with differently tiered PL programs (a “better” program with standard PL vs a full PL quality spectrum with economy, standard and premium PLs) and different PL naming strategies (store-banner name or stand-alone brand name). Design/methodology/approach A survey (N = 644) was used to test the model in the context of the consumer goods retail industry. Exploratory factor analysis, confirmatory factor analysis and multi-group structural equation modelling techniques were used to assess the proposed model. Findings The results show differences in the formation of PL loyalty based on whether the retailer has a tiered PL program. In portfolios with economy, standard and premium PLs, PL associations have a stronger effect than PL awareness in the formation of PL loyalty. Portfolios with a standard PL show balanced effects of PL associations and PL awareness on PL loyalty formation. As to the positive effect of PL brand equity on store loyalty, this study also shows a stronger effect of PL brand equity on store loyalty in chains that choose to use their store banner name in their PLs. Practical implications Retailers that manage multi-tier PL portfolios (as opposed to those that commercialise a standard PL) can increase loyalty to the PL portfolio significantly by constructing highly differentiated images of their economy, standard and premium PLs to ensure that consumers truly perceive the different value propositions of their PL tiers. As to PL naming strategy, the authors recommend that retailers that use the same retail chain name for one or several of their PLs invest in their corporate reputation to strengthen the brand equity achieved by their PLs and thus increase loyalty to the retail chain. Retailers must perform specific communication and advertising campaigns for PLs with the stand-alone brand name. Originality/value Today, any reference to PLs as a whole is overly simplistic, but no research has assessed empirically differences in the influences of a multi-tiered vs a standard PL program on the PL loyalty formation for PL portfolios. Nor has any empirical research incorporated the influence of PL naming strategy on store loyalty. This study fills these gaps, integrating into the same model two significant moderating variables of retailers’ strategy: their PL tier strategy and their PL naming strategy.

Purpose The purpose of this paper is to introduce the concept of customer’s value to the retailer (CVR) from a marketing perspective. CVR is a broad concept that has two components: loyalty intentions to the retailer and intentions to try new products or brands that the retailer offers. This study proposes a theoretical model and considers the effect of three sources of CVR in consumer goods retailing: customer’s perceived functional service value, customer’s perceived private label brand (PLB) equity and customer’s perceived relationship value. The effects of the proposed antecedents are applied to two groups of customers: variety-seeking and non-variety-seeking consumers. Design/methodology/approach The questionnaire surveys were administered to customers of five retail firms with a nationwide presence in Spain (Carrefour, Auchan, Eroski, Mercadona, and El Corte Inglés). A total of 742 valid questionnaires was obtained. Findings The results show that the key differences between variety seekers (VS) and non-variety seekers (NVS) are imposed by PLB equity, which has a stronger relationship to both components of CVR for VS than for NVS. Relationship value has a stronger relationship to intention to try new products or brands for NVS than for VS. Finally, functional service value has a stronger relationship to intention to try new products or brands for VS than for NVS. Practical implications The results obtained for both groups have significant implications for segmentation and management of differentiated marketing strategies based on consumers’ characteristics (variety-seeking tendency). Originality/value This study establishes a new concept and measurement of CVR as determined by the customer him/herself. The paper provides a comprehensive analysis of how the relative importance of service, PLBs and relationships affect CVR. Finally, despite the fact that contemporary consumers tend to seek variety, the variety-seeking profile has not been used to date as a moderating variable in studies of CVR.

As the use of online platforms to search for information about tourist destinations grows, so does the need for destination marketing organizations (DMOs) to offer positive online destination brand (ODB) experiences. Two studies were conducted online to explore the central elements of a positive ODB experience with four official destination platforms (the website, Instagram, Facebook, and Twitter). Based on a photo-elicitation technique, the grounded theory approach, and data triangulation, this study identified six dimensions of a positive ODB experience: sensory, affective, behavioral, intellectual, interactive, and social. Thus, it extends the four-dimensional brand experience scale created by Brakus, Schmitt, and Zarantonello (2009) and proposes two other dimensions that deepen the understanding of an ODB experience: interactive and social. The results indicate that, rather than disseminating content, DMOs should focus on generating sensory, affective, behavioral, and intellectual experiences as well as fostering person-to-person connection and enabling users to create content on destination platforms.

Value co-creation continues to be a key issue in the era of marketing 4.0. Despite an increasing amount of research on value co-creation, there is still a lot of ambiguity in the use of certain terms. For example, different names are used to refer to the same concept. Even though the concept of co-innovation or co-production has nearly the same meaning as co-creation, there are certain differences between them that must be clarified. In addition, another difficulty found in the literature is that the co-creation concept is frequently applied to different objects of study, such that it might be dealing with brand value co-creation, co-creation of experience, co-creation attitude, etc. In order to clarify these issues, this paper presents a brief review of the relevant literature on value co-creation.

Made available in DSpace on 2020-12-12T01:35:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 The tick Rhipicephalus sanguineus sensu lato has great medical and veterinary importance, mainly because the ability to transmit many diseases, causing harm to pets but also risks to public health. The blood spoliation and transmission of pathogens occur because of the immunosuppressive action of these ticks' saliva, a potent mixture of bioactive substances that is secreted by the salivary glands, one of the organs responsible for their biological success, and hence the target of studies for their control. Ozone has promise for use as an alternative acaricide, due to its proven efficiency in controlling agricultural and food pests, besides posing no risk of environmental contamination or to animal and human health. Therefore, this study evaluated the acaricidal potential of exposure of females of R. sanguineus s.l. to ozonated water at many concentrations and analysed the morphophysiological alterations of the salivary glands, employing histological and light microscopic techniques. The results demonstrated that the ozonated water at the concentrations investigated caused severe alterations in the salivary glands, bringing a new perspective for control of R. sanguineus s.l., through an ecologically correct method due to the absence of harm to non-target organisms and the environment. Biology Department Bioscience Institute São Paulo State University “Júlio de Mesquita Filho” UNESP Department of Entomology and Acarology University of São Paulo USP Biology Department Bioscience Institute São Paulo State University “Júlio de Mesquita Filho” UNESP

Natália Rubio Claret Pereira,1,* Rodrigo Azevedo Loiola,1,* Stephen Fernandes Rodrigues,1 Catiuscia P de Oliveira,2 Sabrina L Büttenbender,3 Silvia S Guterres,2 Adriana R Pohlmann,3 Sandra H Farsky1 1Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, Sao Paulo, Brazil; 2Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; 3Department of Organic Chemistry, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil *These authors contributed equally to this work Purpose: The low penetration of drugs across the blood–brain barrier (BBB) compromises the delivery of chemotherapeutic agents to the brain parenchyma and contributes to the poor prognosis of glioblastoma multiforme (GBM). We investigated the efficacy of methotrexate-loaded lipid-core nanocapsules (MTX-LNC) administered by the oral route to treat murine GBM, its ability to cross the BBB, and the mechanisms of MTX-LNC uptake by cultured GL261 glioma and BV2 microglia cells. Materials and methods: Female C57B/6 mice were used in intravital microscopy assays to investigate the penetrance of rhodamine B-label MTX-LNC (RhoB/MTX-LNC) in the brain after oral or IV administration, and to evaluate the BBB integrity. Intracranial implantation of GL261 cells was undertaken to induce a murine GBM model, and the effectiveness of oral MTX or MTX-LNC treatments (started on Day 10 of GBM, every 2 days for 12 days) was quantified by tumor size, body weight, and leukogram. Pharmacological blockade of endocytic pathways was done to investigate the mechanisms of MTX-LNC uptake by cultured GL261 and microglia BV2 cells by using fluorescence microscopy. The effect of MTX-LNC or MTX on GL261 and BV2 proliferation was evaluated to compare the cytotoxicity of such compounds. Results: RhoB/MTX-LNC was detected in brain parenchyma of mice after IV or oral administration, without any damage on BBB. Oral treatment with MTX-LNC reduced tumor volume and prevented weight loss and leukopenia in comparison to MTX-treated mice. MTX-LNC uptake by GL261 is caveolae-dependent, whereas endocytosis of MTX-LNC by BV2 occurs via phagocytosis and macropinocytosis. Both MTX-LNC and MTX reduced GL261 and BV2 proliferation; however, MTX-LNC showed higher efficacy in the inhibition of glioma proliferation. Conclusion: Together, we infer that the higher ability of MTX-LNC to cross the BBB and be captured by cancer and immune brain cells by different mechanisms is responsible for the higher efficacy of oral MTX-LNC treatment in GBM. Keywords: glioma, nanomedicine, blood–brain barrier, microglia, endocytosis

The aim of this study was to analyze the reproductive behavioral responses in Australian Merino rams, to identify those related to a faster training for semen collection with an artificial vagina. Eight Australian Merino rams, aged 1.5 years and with no prior sexual experience, were randomly selected from an extensively grazed flock. One immobilized ewe with no hormone stimulation was used for rams to sexually interact and mount. The frequencies of approaching, sniffing, flehmen, pushing, pawing with chin resting, and tongue flicking were recorded during eight training and three post-training assessments periods. In addition, the duration of sniffing and flehmen responses, as well as the time from when the ram started to approach the ewe until the mount with ejaculation (completed mount) were recorded. Descriptive, correlation, and modeling analyses were performed. Amongst the rams, four mounted the ewe and ejaculated for the first time during the training phase, and three mounted and ejaculated for the first time after the training phase. The remaining ram mounted the ewe and ejaculated for the first time during the post-training evaluation in the following year. A great variability in the behavior repertoire was observed among rams. The correlation analysis indicated that the completed mount was associated with the behaviors during the approaching response. The expression of the sniffing response decreased between the training phase and post-training evaluation, while the responses of pushing the ewe and tongue flicking ceased to occur. Pawing the side of the ewe with the chin resting on the back of the ewe and flehmen responses, however, continued between the training and post-training phases. This led to a decrease in the time from when the ram started to approach the ewe until the completed mount. It is concluded that the responses of approaching the ewe, pawing the side of the ewe with chin resting on the ewe, and sniffing of the ewe (the latter occurring only during the training phase) are behavioral indicators that could be used for selection of easy-to-train rams for purposes of semen collection with an artificial vagina. Fil: Ambrosi, Claudia Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigaciones En Toxicología Ambiental y Agrobiotecnología del Comahue. Universidad Nacional del Comahue. Facultad de Cs.agrarias. Centro de Investigaciones En Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina Fil: Rubio, Brenda Natalia. Universidad Nacional del Comahue. Facultad de Economía y Administración; Argentina Fil: Giménez, Gustavo. Universidad Nacional del Comahue. Facultad de Economía y Administración; Argentina Fil: Venturino, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigaciones En Toxicología Ambiental y Agrobiotecnología del Comahue. Universidad Nacional del Comahue. Facultad de Cs.agrarias. Centro de Investigaciones En Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina Fil: Aisen, Eduardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigaciones En Toxicología Ambiental y Agrobiotecnología del Comahue. Universidad Nacional del Comahue. Facultad de Cs.agrarias. Centro de Investigaciones En Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina Fil: Lopez Armengol, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigaciones En Toxicología Ambiental y Agrobiotecnología del Comahue. Universidad Nacional del Comahue. Facultad de Cs.agrarias. Centro de Investigaciones En Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina

Na Patagonia Norte, os ovinos tem sua estacao de acasalamento iniciada em 15 de marco, portanto, ficam sujeitos as temperaturas do verao. A exposicao de carneiros a estresse termico aumenta a prevalencia de danos microscopicos e anomalias morfologicas nos espermatozoides, que implica uma reducao na fertilidade. Este trabalho avaliou a capacidade adaptativa de carneiros Merino Australiano com la (N = 6) e tosquiados (N = 6): metade ficou ao ar livre e outra metade foi mantida em uma câmara climatica por oito horas, durante cinco dias, chegando gradualmente a uma temperatura maxima de 40 °C. Foram analisados danos microscopicos, anormalidades e alteracoes ultramicroscopicas da membrana plasmatica e do acrossoma da cabeca dos espermatozoides. Os resultados microscopicos confirmaram a existencia de diferenca significativa na porcentagem de espermatozoides sem cauda e com gota citoplasmatica proximal, entre os ejaculados pos-tratamento. A temperatura afetou os carneiros tosquiados, principalmente a cabeca de seus espermatozoides, durante a espermatogenese. Alteracoes submicroscopicas foram observados na membrana plasmatica quando ela estava presente no segmento anterior: quando nao intacta, ficava ondulada ou dilatada. Quando a membrana plasmatica estava ausente, o acrossoma podia se apresentar ondulado ou dilatado. Alem disso, sob efeito do calor, a membrana acrossomal externa pode perder completamente seu conteudo ou apresentar nucleo desnudo. A membrana plasmatica assume uma forma ondulada pelo efeito da temperatura no epididimo. Depois de dez semanas, a cabeca dos espermatozoides recuperou sua forma normal. Como demonstrado neste estudo, a cabeca sem cauda, as gotas citoplasmaticas proximais e as categorias ultramicroscopicas estudadas sao indicadores do efeito do estresse termico no semen, e os carneiros com maior cobertura de la se adaptam melhor ao estresse por calor. Alteracoes de microscopia e de microscopia eletronica de transmissao tem se mostrado excelentes indicadores de estresse por calor em carneiros Merino Australiano e podem ser ferramentas uteis para ajudar criadores de ovelhas a escolher quando comecar a epoca de acasalamento, o que ira variar de acordo com as condicoes ambientais do verao.

Purpose In a world where customer empowerment is continuously increasing and changing the service landscape, retailers must provide memorable shopping experiences to retain and attract new customers. When customers decide to go shopping in physical stores, they expect to enjoy their visit, experiencing cognitive, affective, social, and physical responses evoked by in-store stimuli. The purpose of this paper is to propose and validate a scale to measure in-store customer experience (ISCX). Design/methodology/approach This study’s theoretical review of customer experience (CX) demonstrates that a formative model provides the best structure for measuring the construct ISCX. Furthermore, the study follows the guidelines for rigorous construction of a formative scale, which include three main stages: generation of items, scale purification, and assessment of scale validity and reliability. Findings The results provide evidence that a formative third-order scale with a reflective second-order dimension (social experience) and three reflective first-order dimensions (cognitive, affective, and physical experience) has satisfactory psychometric properties. The findings also provide useful information on the effect of the ISCX scale on key performance variables such as satisfaction and loyalty to the store. Originality/value The ISCX scale proposed constitutes a useful multi-concept diagnostic tool for use by retailers to create fully experiential shopping environments with differential value for the customer. By providing a complete, robust, precise measure of CX in a retail environment, the scale gives researchers a structured way to examine the causes and consequences of CX in retail.

Within the current economic context, store brands play an important role in differentiation strategies based on assortment and positioning in terms of distributor prices. To begin with, this study identifies three determining aspects of retail loyalty and trust: satisfaction with price levels, the perceived image of the assortment and loyalty to the store brands (SB). Secondly, this study proposes a theoretical relational model among the aforementioned aspects. Finally, this study analyzes the moderating role that the strategy of choosing SB name (umbrella brand label vs. brands different from the label brand) plays in the relationship between loyalty to SB and loyalty to and trust in the retailer. By including this moderating variable, we seek to contribute to current academic research and to provide insights into the importance that SB strategy (label vs. own name) has for the effect of loyalty to SB on the relative results of the retail company. Our research results show that making the umbrella SB name the same as the label brand name increases the positive effect of customer loyalty to SB on loyalty to the retailer, but not the effect of trust on this loyalty compared to chains that use SB names different from those of the label. These results have important implications for management.

Loyalty is one of the main assets of a brand. In today's markets, achieving and maintaining loyal customers has become an increasingly complex challenge for brands due to the widespread acceptance and adoption of diverse technologies by which customers communicate with brands. Customers use different channels (physical, web, apps, social media) to seek information about a brand, communicate with it, chat about the brand and purchase its products. Firms are thus continuously changing and adapting their processes to provide customers with agile communication channels and coherent, integrated brand experiences through the different channels in which customers are present. In this context, understanding how brand management can improve value co-creation and multichannel experience-among other issues-and contribute to improving a brand's portfolio of loyal customers constitutes an area of special interest for academics and marketing professionals. This Special Issue explores new areas of customer loyalty and brand management, providing new insights into the field. Both concepts have evolved over the last decade to encompass such concepts and practices as brand image, experiences, multichannel context, multimedia platforms and value co-creation, as well as relational variables such as trust, engagement and identification (among others).

Submitted by NATALIA RUBIO CLARET PEREIRA (natircp@gmail.com) on 2019-08-19T11:53:00Z No. of bitstreams: 1 TESE NATALIA R. C. PEREIRA.pdf: 11063985 bytes, checksum: 71838aee293b7265c14d5148edc027eb (MD5) Approved for entry into archive by Ana Paula Santulo Custódio de Medeiros null (asantulo@rc.unesp.br) on 2019-08-19T18:02:42Z (GMT) No. of bitstreams: 1 pereira_nrc_dr_rcla_par.pdf: 908239 bytes, checksum: bb8a294c4bfc14d584f8234f33e37e80 (MD5) Made available in DSpace on 2019-08-19T18:02:42Z (GMT). No. of bitstreams: 1 pereira_nrc_dr_rcla_par.pdf: 908239 bytes, checksum: bb8a294c4bfc14d584f8234f33e37e80 (MD5) Previous issue date: 2019-06-18 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) O presente trabalho teve como objetivo avaliar o efeito tóxico e terapêutico do extrato da glândula salivar (EGS) de fêmeas de carrapatos Rhipicephalus sanguineus senso lato (Acari: Ixodidae), alimentadas por 2 dias em hospedeiros, em diferentes concentrações, por meio de injeções intraperitoneais em ratas Wistar. Os resultados clínicos mostraram que na concentração de 10 μg/μL houve aumento da frequência de neutrófilos e diminuição da de linfócitos. Na análise histológica do fígado, observou-se que o extrato nessa concentração causou alguns efeitos tóxicos como vacuolização citoplasmática nos hepatócitos e desorganização estrutural dos cordões celulares. Nas concentrações de 40 e 80 μg/μL, as ratas ganharam peso corporal e tiveram o peso relativo do fígado diminuído, evidenciandoo que essas concentrações causaram toxicidade sistêmica. Nesse trabalho foi também analisado o efeito do EGS apenas na concentração de 0,04μg/μL, sobre lesões pré-neoplásicas (LPN) no fígado de ratos Wistar e observou-se aumento no peso relativo apenas do fígado nos animais dos grupos LPN e LPN+EGS quando comparados ao controle. A histologia mostrou que os animais LPN, antes da injeção do extrato, foram os que tiveram o tecido hepático com maiores danos (desorganização dos cordões, aumento de tecido conjuntivo, vacuolização citoplasmática, células apoptóticas e aumento na frequência de células de Kupffer, células de defesa deste órgão) os quais foram reduzidos quando os ratos receberam o EGS. No presente estudo realizou-se ainda a avaliação in vitro do efeito dos acaricidas timol e carvacrol, sobre células RML-15, oriundas de carrapatos R. sanguineus s.l. e observou-se que o timol nas várias concentrações testadas não alterou a viabilidade das mesmas, ao contrário do carvacrol que na maior concentração (0,3 μL/mL) matou mais de 70% das células e na de 0,06μL/mL matou 23% delas. Morfologicamente as células expostas às menores concentrações de timol não apresentaram danos e as expostas as maiores concentrações sofreram: vacuolização citoplasmática, picnose e fragmentação nuclear. O carvacrol foi o composto que causou as maiores alterações morfológicas: perda dos limites celulares, picnose e fragmentação nuclear observadas já na exposição as menores concentrações. Na maior concentração houve 100% de morte das células e na de 0,012uL/mL as células entraram em apoptose, o que caracterizou o efeito citotóxico desse acaricida. This study aimed to evaluate the toxic and pharmacological effect of salivary gland extract (SGE) of female ticks Rhipicephalus sanguineus in different concentrations through intraperitoneal injections in Wistar rats. Clinical results showed that at the concentration of 10 μg/μL there was an increase in neutrophil frequency and a decrease in lymphocytes. It was also observed that in the liver histological analysis, the extract at this concentration caused some toxic effects: cytoplasmic vacuolization in the hepatocytes and structural disorganization in the cellular cords. At concentrations of 40 and 80 μg/μL, rats body weight increased, and relative liver weight decreased, showing that these concentrations caused systemic toxicity. In this study, was analysed the effect of SGE in the concentration of 0.04 μg/μL on the pre-neoplasic lesions (PNL) in the liver of Wistar rats, and there was an increase in the relative weight of the liver in the groups PNL and PNL + SGE. The histology showed that the PNL animals had the hepatic tissue with the biggest damage (cord disorganization, increase of connective tissue, cytoplasmic vacuolization, apoptotic cells and increased Kupffer cell quantity, indicating immune response), those alterations were reduced when the mice received the SGE as treatment. In the study about the in vitro evaluation of the acaricides effect of thymol and carvacrol on RML-15 cells from R. sanguineus s.l. ticks were carried out. Thymol at all tested concentrations did not alter the cells viability, unlike carvacrol that at the highest concentration (0.3 μL/mL) killed more than 70% of the cells and at 0.06 μL/mL it killed 23% of the cells. Morphologically, the cells exposed to the lowest concentrations of thymol presented no damage and the ones exposed to the highest concentrations had cytoplasmic vacuolization, pyknose and nuclear fragmentation. Carvacrol was the compound that caused the highest morphological changes: loss of cell boundaries, pyknosis and nuclear fragmentation observed at the lowest concentrations. At the highest concentration there was 100% cell death, at 0.012 uL/mL the cells entered apoptosis, which characterized the cytotoxic effect of this acaricide. CAPES: 001

Despite an increasing amount of research on cocreation value, research on participants' value-in-use in third-party managed virtual communities remains limited. This study explores how participants perceive value-in-use through their participation in third-party managed virtual communities and the influence of the participants' value-in-use on three cocreation behaviors typical of these communities: information searching, feedback, and coinnovation activities. Participant value-in-use is a multidimensional construct consisting of five dimensions: informational value, entertainment value, social integrative value, personal integrative value, and community interactivity. We also consider whether use of different platforms (website, mobile app, or both) exerts a moderating effect on this cocreation process and identify significant differences in the relationships proposed based on the platform the participants use to access the virtual community. The research is contrasted empirically using the virtual community TripAdvisor. The PLS-SEM method is used to test the model proposed. In comparing the website and mobile app models, the results show significantly stronger effects on the relationships in the route "informational value-participants' value-in-use-information searching" for the website. Also, we find a stronger route for personal integrative value-participants' value-in-use-feedback among users of the mobile app than for website users. In the case of participants who use only one platform (website or mobile app) rather than both (website and mobile app), the weight of use of one technology rather than both is significantly greater in coinnovation. This study enriches previous studies that advance theories of cocreation value and provides companies with practical guidance to identify and encourage cocreation behaviors and enhance the perceived value-in-use of virtual community participants., The authors wish to acknowledge the financial support of the Ministry of Economy and Competitiveness (Research Project Ref. ECO2015-69103-R) and Fundación Ramón Areces (Research Project: “El Capital Cliente en Mercados Minoristas de Gran Consumo”).

Made available in DSpace on 2020-12-12T01:51:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 There are several fixative or preservative solutions for use on cadavers, and formaldehyde is the most widely used. However, this chemical may present negative effects for professionals who manipulate it. Therefore, this study aimed to identify and quantify the main microorganisms related to the fixation and preservation of dog cadavers using an alternative and formaldehyde-free solution. After arterial injection (120 mL kg-1 95% 96° GL ethyl alcohol and 5% pure glycerin), cadavers were placed in 96° GL ethyl alcohol for 30 (group 1), 60 (group 2), 90 (group 3), and 120 days (group 4). After the fixation period, they remained under preservation in a 30% aqueous sodium chloride solution for 120 days. Bacterial quantification was performed by the pour plate method. The bacterial population was present in all groups during fixation, except for group 1, but never exceeded 9 × 101 CFU mL-1 in total aerobes and 7 × 101 CFU mL-1 in total anaerobes. The microbial population was present in all groups in at least two moments during preservation and never exceeded 7 × 101 CFU mL-1 in total aerobes and anaerobes. The presence of fungi was observed in 8 out of 34 analyses. Pseudomonas sp., Escherichia coli, and Bacillus sp. were identified in the analyzed samples. Microbiological counting was low, and no signs of contamination were observed in the vats at visual inspection. Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista UNESP Pós-Graduando em Cirurgia Veterinária UNESP Departamento de Patologia Veterinária UNESP Departamento de Morfologia e Fisiologia Animal UNESP Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista UNESP Pós-Graduando em Cirurgia Veterinária UNESP Departamento de Patologia Veterinária UNESP Departamento de Morfologia e Fisiologia Animal UNESP

The chapter analyzes the components that generate users' engagement in a third-party-managed virtual community and these components' contribution to building user identification with the community. When this chapter distinguish between users who share their comments in multiple service categories to users who share comments in only one or two service categories, it is found that enjoyment benefits and participation in co-innovation increase identification with the community for both groups. The two groups differ, however, in that learning benefits improve identification with the community for participants who share comments on three or more services but not for participants who share comments on fewer services. The latter group's identification is, however, influenced by social benefits, functional benefits, and advocacy, feedback, and help to others.

Even though concepts such as brand experience and online brand credibility are critical in destination marketing strategies, there have been no previous studies that have analyzed their relationships and influence on tourists' behavioral intentions. This paper develops a multimethod approach using a projective technique, an online experiment, and a multigroup analysis with five official destination platforms (the website, Facebook, Instagram, Twitter, and YouTube). The results confirm positive direct and indirect relationships among online destination brand experience (BE), perceived online destination brand credibility (PODBC), and users’ behavioral intentions toward the destination (intentions to visit/recommend). The multigroup analysis that was conducted revealed that users who had not visited the destination shaped their behavioral intentions by assigning a greater importance to the online destination BE than did those who had physically visited the destination. Conversely, users who had visited the destination showed a higher intensity in the path between PODBC and behavioral intentions.

Tourists' intensive use of information and communication technologies when planning travel has forced destination marketing organizations to design online simulacra of destinations in multiple formats. We focus our study on official online destination platforms to analyze preliminary experiences with destination brands and the online perception of authenticity. Previous consumption studies have theorized that consumers' encounters with products/services are antecedents of their perceptions of authenticity. In the tourism literature, however, the link between online destination experiences and online destination authenticity constitutes a research gap. To fill that gap, we used a multimethod approach to develop a causal-predictive model by which we observed that the online destination brand experience directly affects destination brand authenticity. The findings also show that both of these constructs directly and indirectly influence users' behavioral intentions toward the destination. We examine the moderating role of various official online destination platforms to enrich the theoretical and managerial implications discussed.

This paper proposes a theoretical model that investigates the formation of store brandś equity (SBs) and its influence on the loyalty to the retailer considering the role that the commercial format chosen by the retailer (supermarkets versus hypermarkets) can have in this process. The results obtained reveal one important data regarding the proposed modelling between both groups. While the formation process of store brands’ equity is identical for supermarkets and hypermarkets, store brands’ equity as source of retailer loyalty is significantly more important for supermarkets. Important implications for management are derived from the results obtained. For supermarkets’ managers, generate preference for their own brands is a key issue, and, without underestimating the importance of a proper management of their own brands for hypermarkets’ managers, they should focus on the implementation of other marketing tools to generate loyalty to the retailer, such as a pleasant in-store experience, purchase convenience and a wide assortment.

Purpose The purpose of this paper is to attempt to understand the extent to which price consciousness and quality consciousness influence attitudinal loyalty to store brands (SBs) in different segments of consumers: heavy, medium and light buyers of SBs. SBs are currently consolidated among price-conscious consumers, but less established among the quality-conscious consumers. Design/methodology/approach After reviewing the literature and constructing a theoretical model, the authors performed a study on Spanish food products, a sector in which SBs have achieved a significant market share. They collected data through a personal survey and analyzed it using structural equations modeling, and they performed a multigroup analysis of heavy buyers, medium buyers and light buyers of SBs. Findings The results obtained alert retailers to the tremendous importance of price vs quality in the formation of SB value and loyalty to SBs among heavy buyers of these brands, show the balance between price and quality as components of SB value and generators of loyalty among medium buyers and recognize the need to strengthen the image of SB quality to reinforce SBs’ value and smart shopping associations to increase light buyers’ loyalty to SBs. Originality/value The study contributes new evidence and knowledge on SB loyalty among consumers who show different usage of these brands (heavy, medium and light buyers). It then assesses the short- and long-term value of each segment of customers for the retailer and recommends retail strategies adapted to each segment.