1. Novel angular naphthopyrone formation by Arp1p dehydratase involved in Aspergillus fumigatus melanin biosynthesis
- Author
-
Tomoki Yoshida, Yun C. Chang, Yutaka Ebizuka, Hiroshi Tomoda, Huei-Fung Tsai, K. J. Kwon-Chung, Natsuki Nambu, Isao Fujii, and Nobutada Tanaka
- Subjects
Melanins ,biology ,Chemistry ,Aspergillus fumigatus ,biology.organism_classification ,Agricultural and Biological Sciences (miscellaneous) ,Article ,Yeast ,Virulence factor ,Fungal Proteins ,Kinetics ,Biochemistry ,Dehydratase ,Polyketide synthase ,Gene cluster ,Escherichia coli ,biology.protein ,Humans ,Magnaporthe grisea ,Gene ,Hydro-Lyases ,Ecology, Evolution, Behavior and Systematics - Abstract
Conidial pigment is an important virulence factor in Aspergillus fumigatus, a human fungal pathogen. The biosynthetic gene cluster for 1,8-dihydroxynaphthalene (DHN)-melanin in A. fumigatus consists of six genes, alb1, ayg1, arp1, arp2, abr1, and abr2. In contrast to black DHN-melanin fungi such as Magnaporthe grisea, the polyketide synthase Alb1p in A. fumigatus produces naphthopyrone YWA1 instead of 1,3,6,8-THN (T4HN) and YWA1 is converted to T4HN by Ayg1p. The yeast transformant expressing Alb1p and Arp1p dehydratase produced an unknown compound which was identified to be a novel angular naphthopyrone named YWA3 formed from YWA1. In addition, the amount of YWA3 produced was much more than that of YWA2 formed by non-enzymatic dehydration from YWA1. To further analyze the reaction in vitro, Arp1p was overexpressed in E. coli and purified. Kinetic analysis revealed Km value of Arp1p for YWA1 to be 41 μM which is comparable with that of Ayg1p for YWA1 in conversion to T4HN. The complex structure modelling well explained the mechanism of YWA3 generation by the dehydration of angular YWA1 by Arp1p. These results indicated the possibility that polymerization of angular naphthopyrone YWA3 but not YWA2 could be involved in the characteristic bluish-green conidial pigmentation of A. fumigatus.
- Published
- 2021
- Full Text
- View/download PDF