1. Genome-wide view and characterization of natural antisense transcripts in Cannabis Sativa L.
- Author
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Zhang, Chang, Jiang, Mei, Liu, Jingting, Wu, Bin, and Liu, Chang
- Abstract
Natural Antisense Transcripts (NATs) are a kind of complex regulatory RNAs that play crucial roles in gene expression and regulation. However, the NATs in Cannabis Sativa L., a widely economic and medicinal plant rich in cannabinoids remain unknown. In this study, we comprehensively predicted C. sativa NATs genome-wide using strand-specific RNA sequencing (ssRNA-Seq) data, and validated the expression profiles by strand-specific quantitative reverse transcription PCR (ssRT-qPCR). Consequently, a total of 307 NATs were predicted in C. sativa, including 104 cis- and 203 trans- NATs. Functional enrichment analysis demonstrated the potential involvement of the C. sativa NATs in DNA polymerase activity, RNA-DNA hybrid ribonuclease activity, and nucleic acid binding. Finally, 18 cis- and 376 trans- NAT-ST pairs were predicted to produce 621 cis- and 5,679 trans- small interfering RNA (nat-siRNAs), respectively. These nat-siRNAs were potentially involved in the biosynthesis of cannabinoids and cellulose. All these results will shed light on the regulation of NATs and nat-siRNAs in C. sativa.Key message: In C. sativa, NATs potentially participated in genetic information processes, growth and development, stress resistance, and the biosynthesis of compounds. Nat-siRNAs were potentially involved in the biosynthesis of CBDs and cellulose. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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