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The increasing food crisis in times of ecological threats has challenged conventional agriculture to transform into a more efficient and sustainable agri-food system. The global priority of these activities has become, among others, the responsible and effective use of already produced food. This study aimed to assess the impact of the natural resistance of cereal grains to consumption by storage pests. The study presented here analyzed the impact of selected chemical factors from the grain of six species of cereals (wheat, triticale, rye, barley, oat, corn) on the development of one of the most dangerous storage pests—the lesser grain borer Rhyzopertha dominica F. The increased development of this beetle on the tested grain was determined based on the number of progeny, the mass of dust produced, and the loss of grain mass. Moreover, the correlations between the above-mentioned development parameters of the pest and the content in the grain of dry matter, crude ash, total protein, crude fat, starch, and water-soluble carbohydrates (WSCs) were examined. The results showed that the tested pest developed most intensively on barley and triticale grains and was least abundant on oat and corn grains. Chemical analysis of the selected grain showed that the low number of the R. dominica progeny population was correlated with a higher crude fat content and a lower total protein content in the cereal grain, and therefore, these chemical properties could affect the development of the pest. Knowledge of these relationships can be used in cereal breeding programs and when recommending cereals for more extended storage. This directly translates into improved local and global nutritional and food security. Moreover, it may also contribute to the reduction of pesticide use at the storage stage, which is one of the basic requirements for agricultural production in a sustainable agriculture system. [ABSTRACT FROM AUTHOR]

Microtus fortis is known as a non-susceptible animal host of S. japonicum. A better understanding of this animal immune defense mechanism during the early stage of infection may offer an alternative route for vaccine development or therapy. Here, we analyzed the whole blood transcriptome of M. fortis using next-generation sequencing (NGS) to identify immune genes of biological relevance that might be involved in the mechanism of its resistance. The blood samples were collected from uninfected animals (control group) and infected animals at different time points (3, 7, 10 and 14 days post-infection). We identified 5310 sequences as unigenes and successfully annotated 4636 of them. The immune response was more intense at 10 dpi. The upregulated genes at this time point were mainly activated in the TNF and NF-kappa B signaling pathways, Th1, Th2and Th17 cell differentiation as well as cytokine-cytokine receptor interaction. Based on the differentially expressed genes analysis, we report that the IF27L2B, RETN, PGRP, IFI35, TYROBP, S100A8, S100A11, CD162, CD88, CYBA, and LBP could play important roles in the mechanism of M. fortis resistance.

The research has covered the biological wood resistance in terms of reducing its wood density of Silver fir growing in the Ukrainian Carpathians. Six Silver fir stands were placed in the State Enterprise Perechyn Forestry, in which were cut 54 model trees with varying decay stages. All qualitative characteristics of Silver fir wood were diagnosed by the European norms. Altogether, 1620 wood samples were studied. The start of biological damage to wood was defined as the cessation of cambial activity and drying of the tree crown, as well as diagnosed fungal lesions. As a result, there were significant di!erences in wood density with a di!erent timeframe of decay stages of wood. The basic density of healthy wood varied from 362 kg·m-3 to 457 kg·m-3 with an average value of 392 kg·m-3, and the same figure for wood with significant damage ranged from 195 kg·m-3 to 283 kg·m-3 with an average value of 246 kg·m-3. This should be associated with the biological destruction of the cell wall a!ected by fungi from division Basidiomycota. Four significant decay stages of Silver fir stemwood taking the lesioned time by the equal variances estimated through Scheé's Method were set: (1) healthy wood without signs of biological damage by fungi; (2) initial wood damage caused by wood-staining fungi within up to 6 months; (3) medium wood damage caused by wood-destroying fungi within the timeframe from 0.5 to 2 years; and (4) substantial wood damage caused by wood-destroying fungi for more than 2 years. [ABSTRACT FROM AUTHOR]

There have been studied the influence of feeding plant raw material obtained from Hungarian sainfoin having an immunostimulating effect on the growth rate of dairy calves, as well as on morphological and biochemical blood parameters and natural resistance of the animal organism. Calves of the I and II experimental groups in addition to the main diet were fed a phyto supplement made of Hungarian sainfoin mixed with concentrates in doses of 150 and 300 g per head per day during 92 days. The use of herbal supplements in feeding calves ensured an increase in live weight in the animals of experimental groups by 11.0 kg (6.55 %) and by 11.7 kg (6.96 %) (p

Grapevine trunk diseases (GTDs) are taking the forefront of winegrowers' concerns, as they cause considerable damage in vineyards not only quantitatively but also qualitatively. Furthermore, in the last three decades, an increasing incidence of the socioeconomic impact of these fungal pathologies has been observed. To date, no effective control strategies or curative treatments are available for these diseases that can replace the effectiveness offered by a series of chemical synthesis fungicides currently prohibited by European legislation. In this scenario, screening for less sensitive cultivars is regarded as a sustainable approach for GTDs management. In the study presented herein, the tolerance/susceptibility of 25 cultivars from the Grapevine Germplasm Bank of Aragon (Movera, Zaragoza, Spain) including commercial, local, or minority germplasm, was tested against two pathogens associated with Botryosphaeria dieback (viz. Neofusicoccum parvum and Diplodia seriata), which were inoculated in a detached cutting assay under open-air conditions. Based on lesion length development after eight months, significant differences were detected among the cultivars in the length of internal (vascular) necroses. In general terms, all cultivars were susceptible to fungal infection, but 'Macabeo' and one of the 'Garnacha Tinta' ecotypes under study (from Villanueva de Huerva, Zaragoza) would be the least susceptible white and red cultivars, respectively. On the other hand, 'Monegrina', 'Grumel', and 'Torcijón' would be among the least tolerant cultivars to fungal infection. [ABSTRACT FROM AUTHOR]

Using probiotic preparations and their complexes in poultry farming is an important step towards increasing poultry resistance and productivity, and thus producing environmentally friendly products. The purpose of the study was to investigate the effect of the complex action of symbiotic drugs and biocidal agents by investigating the clinical and biochemical parameters of blood and mineral metabolism in the body of broiler chickens under the conditions of their use according to the developed scheme. Hematologic, biochemical and immunologic blood parameters were explored by conventional methods, micro- and macroelements by optical emission spectrometry with inductively coupled plasma. Using the proposed complex of symbiotic and biocidal agents in the technology of rearing broiler chickens activates the processes of erythropoiesis, haemoglobin production, restoration of protein metabolism and normalisation of mineral status within physiological limits, which indicates the strengthening of the body's defences, increased resistance to stress factors and adverse environmental conditions, and will further have a positive effect on the growth and productivity of the target bird. The pronounced effect of the complex combination of drugs on the body of broiler chickens of experimental B is a better assimilation of many inorganic elements (by increasing the content of Calcium, Phosphorus, Cuprum, Ferrum and Selenium, Magnesium, Manganum); and, accordingly, causes the sustainable development of metabolic processes due to the prevalence of anabolic over catabolic processes (by increasing the level of total proteins against the background of a physiological increase in the number of globulin fractions; including the induction of the endogenous detoxification system – by reducing the level of production of toxic metabolites of purine metabolism: uric acid and creatinine; reduction of Chromium content; normalisation of transamination processes and deceleration of alkaline phosphatase activity) and immunobiological reactions (by enhancing erythropoiesis and haemoglobin production; induction of lysozyme, bactericidal and phagocytic activity), respectively. The proposed system of rearing young poultry using a complex of symbiotic preparations in combination with biocidal agents promotes the synergistic effect of the latter and their effective action on the body of experimental broiler chickens, which increases nonspecific resistance and restores metabolic reactions

Indian sandalwood (Santalum album L.) is a semi-parasitic tree, well known for its valuable sandalwood oil extracted from heartwood and roots. Extensive loss of heartwood as a result of its decay is observed in living trees and this has inspired the current study, to investigate the health of sandalwood in Marayur sandalwood forest reserves. Located in Kerala, India, the majority of the trees in Marayur are facing high mortality with the loss of heartwood. The current study evaluated the health status and heartwood-rot disease severity of each tree, which revealed that only 16.67 % of the total trees were healthy and 1.5 % of them were dead. Statistical analysis revealed increasing girth class of trees has a significant positive association with increased disease severity at a 0.05 level of significance. Laboratory analysis confirmed the presence of the major wood-rotters Allophoma tropica , Xylaria berteroi , Diaporthe sp. Fomes sp. Ganoderma appalanatum , and Trametes sp. and that they are collectively responsible for the heart rot. A durability assessment revealed sandalwood is highly resistant (durability class I) to white-rot fungi (viz. Trametes hirsutus and T. [ Coriolus ] versicolor) and moderately resistant (durability class II) to brown-rot fungi (Oligoporous placenta and Fomitopsis meliae). Study Implications : This study highlights the health status and heart-rot disease severity of sandalwood trees in Marayur Forest Reserves. Of the total trees surveyed, about 1.5% mortality was observed and only 17% of the trees were devoid of any heart-rot symptoms. Allophoma tropica , Ganoderma applanatum , Xylaria berteroi isolate SR52, Diaporthe sp. Fomes sp. G. appalanatum , and Trametes sp. are typical species collectively responsible for the heart rot and this was found to be the case in our study. These findings will sensitize forest managers to the loss of valuable sandalwood and death of the trees due to the heart rot, thereby enabling them to take appropriate preventive measures. Also, the natural durability of sandalwood against brown- and white-rot fungi is reported for the first time, indicating that sandalwood falls in the resistance class I (highly resistant) against white-rot fungi (Trametes hirsutus and T. (Coriolus) versicolor) and resistance class II (resistant) against brown-rot fungi (Oligoporous placenta and Fomitopsis meliae). [ABSTRACT FROM AUTHOR]

Plant viruses, as obligate intracellular parasites, rely exclusively on host machinery to complete their life cycle. Whether a virus is pathogenic or not depends on the balance between the mechanisms used by both plants and viruses during the intense encounter. Antiviral defence mechanisms in plants can be of two types, i.e., natural resistance and engineered resistance. Innate immunity, RNA silencing, translational repression, autophagy-mediated degradation, and resistance to virus movement are the possible natural defence mechanisms against viruses in plants, whereas engineered resistance includes pathogen-derived resistance along with gene editing technologies. The incorporation of various resistance genes through breeding programmes, along with gene editing tools such as CRISPR/Cas technologies, holds great promise in developing virus-resistant plants. In this review, different resistance mechanisms against viruses in plants along with reported resistance genes in major vegetable crops are discussed. [ABSTRACT FROM AUTHOR]

Tectona grandis Linn. f. (teak) is one of the most appreciated high-quality timber all over the world due to its economic value and wide array of applications. This tropical hardwood presents outstanding characteristics like pleasing aesthetic appearance, strength, lightness, ease of working, dimensional stability, and decay resistance. The latter quality is mainly ascribed to its extractives, which contain biologically active compounds (mainly quinones and anthraquinones) that confer a natural resistance against termites and fungi. This review focuses on teak secondary metabolites and the bioactivity potential of heartwood extractives. Furthermore, it covers the generalities of the teak tree and gives an overview on the approaches aimed to valorize the wastes from woodworking enterprises as a possible source of functional extractives and as an eco-friendly raw material. Notwithstanding the efforts made to elucidate the compounds present in teak wood, further research is needed to understand the chemical bases of its natural resistance to decay. Moreover, there is a lack of economic, technical, and ecotoxicity feasibility studies regarding extractives as a source of bioactive molecules for pharmaceutical, food, or cosmetics purposes.

Rapamycin is a lipophilic macrolide antibiotic which is famous for its immunosuppressive and anticancer activity. In recent years, rapamycin showed significant activity against various plant pathogenic fungi. However, the sensitivity of Colletotrichunm fungi to rapamycin is scarcely reported. In this study, we evaluated the sensitivity of 116 Colletotrichum isolates from tea-oil trees to rapamycin. Most isolates exhibited natural resistance with inhibition rates of 50 to 70% at 50 μg/mL. Three Colletotrichum camelliae isolates were found to be sensitive to rapamycin. No mutations were detected in the direct target FKBP12 and indirect target TOR-FRB domain of resistant and sensitive C. camelliae isolates. Notably, the expression of the TOR homolog (CcTOR) was higher in resistant C. camelliae isolates compared to the sensitive ones and overexpression of CcTOR in the sensitive isolate CcS1 resulted in decreased sensitivity to rapamycin. Moreover, ribosomal protein S6 phosphorylation was abolished in the sensitive isolate CcS1 but not in the resistant isolate CcR1 under rapamycin treatment. In addition, the expression levels of ribosome biogenesis genes and two other components of TORC1 were higher in CcR1 compared to CcS1 under the same treatment, which suggested that the abundance of TORC1 in CcR1 was greater than in CcS1, leading to more active TORC1 signaling in CcR1. These results provided a better understanding about natural resistance of C. camelliae isolates to rapamycin and could help for developing new TORC1 signaling-targeting fungicides. [Display omitted] • Most of the Colletotrichum isolates exhibited natural resistance to rapamycin. • No mutations were found in CcFKBP12 and CcTOR-FRB domian. • Expression of TORC1 components were higher in the resistant C. camelliae isolates. • TORC1 signaling were more active in the resistant C. camelliae isolates. [ABSTRACT FROM AUTHOR]

Due to its high degree of natural resistance to terbinafine in vitro and its tendency to spread globally from the Indian subcontinent, the emerging dermatophyte Trichophyton indotineae has become a major concern in dermatology. Herein, we present the first report of T. indotineae from mainland China. The transmission of the fungus to Guizhou Province in central China and eventual host susceptibilities were investigated. We studied 31 strains of the T. mentagrophytes complex from outpatient clinics of our hospital collected during the past 5 years. The set comprised four ITS genotypes, two of which were T. mentagrophytes genotype VIII, now known as Trichophyton indotineae; the earliest isolation in the Guiyang area appeared to date back to 2018. The isolate was derived from an Indian patient, while local Chinese patients had no dermatophytosis caused by this genotype. Reports from around the world indicated that almost all of the globally reported T. indotineae cases originated from the Indian subcontinent and surrounding countries without transmission among native populations, suggesting deviating local conditions or racial differences in immunity against this fungus.

The western corn rootworm (WCR), Diabrotica vifgirera virgifera LeConte, (Coleoptera: Chrysomelidae) causes significant economic damage in corn production each year. Resistance to insecticides and transgenic corn with Bacillus thuringiensis (Bt), Berliner toxins have been reported throughout the United States Corn Belt. Corn breeding programs for natural resistance against WCR larvae could potentially assist in rootworm management. Root damage and root regrowth of eight corn lines were evaluated in field assays at three different locations. Results indicated the inbred 'Mp708' had the greatest root damage and was significantly greater than damage for the susceptible control, B37×H84. In greenhouse assays, we evaluated four of these lines plus a hybrid expressing the mCry3A Bt toxin for damage. The results indicated that Mp708 had significantly higher root damage when compared to 'CRW3(S1)C6' and 'MIR604'. Despite previous work suggesting otherwise, we conclude that Mp708 is highly susceptible to the WCR larvae based on root damage in field and greenhouse plant assays. [ABSTRACT FROM AUTHOR]

Microtus fortis is known as a non-susceptible animal host of S. japonicum . A better understanding of this animal immune defense mechanism during the early stage of infection may offer an alternative route for vaccine development or therapy. Here, we analyzed the whole blood transcriptome of M. fortis using next-generation sequencing (NGS) to identify immune genes of biological relevance that might be involved in the mechanism of its resistance. The blood samples were collected from uninfected animals (control group) and infected animals at different time points (3, 7, 10 and 14 days post-infection). We identified 5310 sequences as unigenes and successfully annotated 4636 of them. The immune response was more intense at 10 dpi. The upregulated genes at this time point were mainly activated in the TNF and NF-kappa B signaling pathways, Th1, Th2and Th17 cell differentiation as well as cytokine-cytokine receptor interaction. Based on the differentially expressed genes analysis, we report that the IF27L2B , RETN , PGRP , IFI35 , TYROBP , S100A8 , S100A11 , CD162 , CD88 , CYBA , and LBP could play important roles in the mechanism of M. fortis resistance., Competing Interests: We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome., (© 2024 The Authors.)

Objective: The research was conducted to study the effect of a complex antimicrobial drug with an anti-inflammatory effect and an antimicrobial drug with an immunostimulating effect on the parameters of nonspecific resistance in calves. Materials and Methods: Two groups (n = 5 each) of sick calves with respiratory pathology were selected for this study. For the treatment of the first experimental group, a complex antimicrobial drug Sulfetrisan® was used. The second experimental group of the calves was intramuscularly injected with the experimental drug gentaaminoseleferon (GIA). To assess the cellular component of immunity in the blood before and after treatment, the number of white blood cells, T-lymphocytes, B-lymphocytes, phagocytic activity of leukocytes, phagocytic number, and phagocytic index (PhI) were determined. In addition, for assessing the humoral component, serum complement activity (SCA), serum lysozyme activity, serum bactericidal activity (SBA), circulating immune complexes (CIC), and total immunoglobulins (total Ig) were measured. The results were compared with the baseline parameters of healthy calves of the control group. Results: When studying the parameters of the humoral and cellular components of nonspecific resistance, it was found that in sick animals, compared with healthy ones, respiratory pathology was accompanied by an imbalance in the immune system. In the process of recovery in animals of the experimental groups under the effect of the drugs, positive changes occurred. However, many of the studied parameters did not reach the values of healthy animals. In the group of calves that received GIA, compared with the calves given Sulfetrisan®, a significant increase in PhI (p < 0.05), SBA (p < 0.006), SCA (p < 0.05), total Ig (p < 0.0005), and CIC (p < 0.05) was observed, which indi¬cated an increase in natural resistance due to the immunostimulating action. Conclusion: The use of GIA in sick animals added to an increase in the general nonspecific cellular and humoral resistance of calves, which made it possible to increase therapeutic efficacy and shorten their recovery time. [J Adv Vet Anim Res 2021; 8(3.000): 355-360]

This work compared the presence of phospholipase A 2 inhibitors (PLIs) in the serum of 19 snake species maintained at Instituto Butantan to better understand the mechanisms of venom resistance in snakes and improve the treatment of snakebite. PLI was isolated from blood of 19 snake species by one-step chromatography and identified in all samples, besides its identity was confirmed through the interaction with both phospholipase A 2 and anti-γPLI. These findings highlight the diversity of snake serum PLIs and emphasize the importance of structure-function studies. [Display omitted] • Isolation and characterization of the PLIs in 8 new Bothrops snake species. • PLIs showed only one or two bands of 25 kDa monomers in all Bothrops , Crotalus and Boa , differing to N. kaouthia pattern. • Variable numbers of the spots in 2D electrophoresis suggested a variable isoform composition of the PLIs in snake blood. • The immunoreactivity assays confirmed the proteins as PLIs, evidencing the interaction with the PLA 2 from C. d. terrificus. [ABSTRACT FROM AUTHOR]

Measuring the natural resistance of wood is fundamental for proper use. The natural durability of five tropical wood species was investigated by field decay testing during exposure for 360 days. Wood logs (length of 0,5 m; diameter of 8 cm - 12 cm) were used in this study. The mass loss and decay index were calculated and visual analysis during the exposure time was performed for all samples. The samples presented evidence of two different groups concerning natural durability. The species in the first group (Mimosa caesalpiniifolia, Mimosa ophthalmocentra, and Mimosa tenuiflora) showed the highest resistance to biodeterioration, better or similar performance compared to treated eucalyptus wood (as control). The other group (Aspidosperma pyrifolium and Cordia oncocalyx) had lower natural resistance in outdoor service, being more susceptible to decay. In general, the wood of the first group is indicated for outdoor uses that require medium or prolonged exposure, such as timber stakes and fence posts.

Objectives: Probiotics are well documented for their health benefits by developing a balanced intestinal microbiota and boosting immunity. The present study was conducted to determine the effects of a probiotic preparation EnzimsporinTM (consisting of spore-forming bacteria Bacillus subtilis B-2998D, B-3057D, and Bacillus licheniformis B-2999D) on the biochemical, hematological, immunological parameters, intestinal microbiota, and growth dynamics of sheep and lambs. Materials and methods: Enzimsporin was fed to lambs and sheep at different doses to determine the bacterias probiotic effects. Sheep were divided into three groups (six each), which received 0, 1, and 3 gm of Enzimsporin/per head/day, respectively, and two groups of lambs (10 each), who received 0 gm and 1 gm of Enzimsporin/per head/day for 30 days in addition to their regular ration. On day 30, blood samples were collected, followed by the determination of biochemical, hematological, and natural resistance indicators. Fecal samples were examined to determine the intestinal microflora, and animals were weighed daily to determine their growth dynamics. Results: Supplementation of probiotics (EnzimsporinTM) improved the lambs body weight gain by 18.8%. Analysis of the clinical parameters showed improvements in the levels of total protein, globulins, and urea by 5.3%, 10.8%, and 6.2%, respectively, in the blood of probiotic-supplemented lambs. Similarly, an increment in the total protein, albumins, and globulins was observed in the sheep with EnzimsporinTM supplementation. The decrease in bilirubin and cholesterol levels in the blood and increased bactericidal and phagocytic index in the sheep and lambs with probiotic supplementation indicated a positive influence of EnzimsporinTM on the liver function and natural resistance. Furthermore, an increase in Lactobacillus and Bifidobacterium and a decrease in the Escherichia coli, Enterococcus, and Yeast in the fecal contents of experimental sheep and lambs indicated the potentiality of EnzimsporinTM on maintaining good gut health. Conclusion: Spore-forming bacteria B. subtilis B-2998D, B-3057D, and B. licheniformis B-2999D can be used in feeding sheep and lambs of 2 months of age to increase body weight gain, improve intestinal microbiota, strengthen the immune system, and maintain normal metabolic processes. [J Adv Vet Anim Res 2021; 8(1.000): 146-157]

Plant viruses, as obligate intracellular parasites, rely exclusively on host machinery to complete their life cycle. Whether a virus is pathogenic or not depends on the balance between the mechanisms used by both plants and viruses during the intense encounter. Antiviral defence mechanisms in plants can be of two types, i.e., natural resistance and engineered resistance. Innate immunity, RNA silencing, translational repression, autophagy-mediated degradation, and resistance to virus movement are the possible natural defence mechanisms against viruses in plants, whereas engineered resistance includes pathogen-derived resistance along with gene editing technologies. The incorporation of various resistance genes through breeding programmes, along with gene editing tools such as CRISPR/Cas technologies, holds great promise in developing virus-resistant plants. In this review, different resistance mechanisms against viruses in plants along with reported resistance genes in major vegetable crops are discussed.

Tectona grandis Linn. f. (teak) is one of the most appreciated high-quality timber all over the world due to its economic value and wide array of applications. This tropical hardwood presents outstanding characteristics like pleasing aesthetic appearance, strength, lightness, ease of working, dimensional stability, and decay resistance. The latter quality is mainly ascribed to its extractives, which contain biologically active compounds (mainly quinones and anthraquinones) that confer a natural resistance against termites and fungi. This review focuses on teak secondary metabolites and the bioactivity potential of heartwood extractives. Furthermore, it covers the generalities of the teak tree and gives an overview on the approaches aimed to valorize the wastes from woodworking enterprises as a possible source of functional extractives and as an eco-friendly raw material. Notwithstanding the efforts made to elucidate the compounds present in teak wood, further research is needed to understand the chemical bases of its natural resistance to decay. Moreover, there is a lack of economic, technical, and ecotoxicity feasibility studies regarding extractives as a source of bioactive molecules for pharmaceutical, food, or cosmetics purposes. [ABSTRACT FROM AUTHOR]

Respiratory diseases of animals are a significant problem in veterinary medicine. They cause a lot of economic damage to farms, consisting of decreasing productivity and breeding qualities of animals and their deaths. The significant spread of respiratory diseases in animals is caused by decreasing natural resistance due to violation of the housing technology, as well as a high concentration of opportunistic and pathogenic microflora in the indoor air. The results of individual therapy in calves with nonspecific catarrhal bronchopneumonia with the use of the drug Calfmin, which is based on compounds of biogenic micro- and macroelements, and plant immunomodulators, are presented. The clinical parameters of calves, namely, habitus, condition of hair, skin, visible mucous membranes, superficial lymph nodes, body temperature, number of respiratory movements, and heart contractions were studied during bronchopneumonia and their changes under individual treatment of animals. The indicators of protein metabolism in the blood serum of clinically healthy and calves with nonspecific bronchopneumonia were also studied. The content of total protein, albumin, α-, β- and γ-globulins was examined in the blood serum of calves by nephelometric method using phosphate buffers. The dynamics of the comparative analysis of total protein content and its fractions in the blood serum between calves of separate experimental groups is shown. The results of studies indicate a decrease in the body's natural resistance and suppression of immunity in sick calves and the rapid recovery under the conditions of the application of nanoaquachelate macronutrients in the form of the drug Calfmin in combination with Echinacea

Not all individuals exposed to a pathogen develop illness: some are naturally resistant whereas others develop an asymptomatic infection. Epidemiological studies suggest that there is similar variability in susceptibility to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. We propose that natural resistance is part of the disease history in some individuals exposed to this new coronavirus. Epidemiological arguments for natural resistance to SARS-CoV-2 are the lower seropositivity of children compared to adults, studies on closed environments of ships with outbreaks, and prevalence studies in some developing countries. Potential mechanisms of natural resistance include host genetic variants, viral interference, cross-protective natural antibodies, T cell immunity, and highly effective innate immune responses. Better understanding of natural resistance can help to advance preventive and therapeutic measures against infections for improved preparedness against potential future pandemics. Natural resistance against pathogens is described for many infections. There is no reason to believe that this might be different for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. There are discrepancies between different SARS-CoV-2 infection models and the seroprevalence of antibodies in the circulation. Differences in morbidity and mortality between countries suggest that genetic and environmental differences may influence host defense against SARS-CoV-2 infection. Studies on children, adults residing in confined environments, and on individuals residing in different geographical locations suggest that natural resistance against SARS-CoV-2 is present in human populations, and may differentially impact on the course of the pandemic in such populations. Crucial for host survival is the balance between resistance mechanisms aiming to eliminate host pathogens and tolerance mechanisms aiming to avoid collateral damage induced by inflammatory processes. [ABSTRACT FROM AUTHOR]

Mycobacterium bovis is the main cause of bovine tuberculosis (BTB) in cattle and can also infect humans. Zebu cattle are considered more resistant to some infectious diseases compared with Holstein‐Friesian (HF) cattle, including BTB. However, epidemiological studies may not take into account usage differences of the two types of cattle. HF cattle may suffer greater metabolic stress due to their more or less exclusive dairy use, whereas Zebu cattle are mainly used for beef production. In experiments conducted so far, the number of animals has been too small to draw statistically robust conclusions on the resistance differences between these cattle breeds. Here, we used a BCG challenge model to compare the ability of naïve and vaccinated Zebu and HF cattle to control/kill mycobacteria. Young cattle of both breeds with similar ages were housed in the same accommodation for the duration of the experiment. After correcting for multiple comparisons, we found no difference between naïve HF and Zebu (ρ = 0.862) cattle. However, there was a trend for vaccinated HF cattle to have lower cfu numbers than non‐vaccinated HF cattle (ρ = 0.057); no such trend was observed between vaccinated and non‐vaccinated Zebu cattle (ρ = 0.560). Evaluation of antigen‐specific IFNγ secretion by PBMC indicated that Zebu and HF cattle differed in their response to mycobacteria. Thus, whilst there may be difference in immune responses, our data indicate that with the number of animals included in the study and under the conditions used in this work, we were unable to measure any differences between Zebu and HF cattle in the overall control of mycobacteria. Whilst determination of different susceptibilities between Zebu and HF cattle using the BCG challenge model will require larger numbers of animals than the number of animals used in this experiment, these data should inform future experiments. [ABSTRACT FROM AUTHOR]

Blast diseases, caused by the fungal pathogen Magnaporthe oryzae, are among the most destructive diseases that occur on at least 50 species of grasses, including cultivated cereals wheat, and rice. Although fungicidal control of blast diseases has widely been researched, development of resistance of the pathogen against commercially available products makes this approach unreliable. Novel approaches such as the application of biopesticides against the blast fungus are needed for sustainable management of this economically important disease. Antagonistic microorganisms, such as fungi and probiotic bacteria from diverse taxonomic genera were found to suppress blast fungi both in vitro and in vivo. Various classes of secondary metabolites, such as alkaloids, phenolics, and terpenoids of plant and microbial origin significantly inhibit fungal growth and may also be effective in managing blast diseases. Common modes of action of microbial biocontrol agents include: antibiosis, production of lytic enzymes, induction of systemic resistance in host plant, and competition for nutrients or space. However, the precise mechanism of biocontrol of the blast fungus by antagonistic microorganisms and/or their bioactive secondary metabolites is not well understood. Commercial formulations of biocontrol agents and bioactive natural products could be cost-effective and sustainable but their availability at this time is extremely limited. This review updates our knowledge on the infection pathway of the wheat blast fungus, catalogs naturally occurring biocontrol agents that may be effective against blast diseases, and discusses their role in sustainable management of the disease. [ABSTRACT FROM AUTHOR]

Varroa destructor is arguably the most important threat to Apis mellifera honey bees. Despite the recentness of the invasion of Varroa, A. mellifera colonies naturally resistant to the mite are being observed in a growing number of populations across Europe, South Africa and Brazil. Appearing in concert with this resistance is an increase in the ability of workers to detect mite-infested cells, which is closely associated with the recapping of such cells. However, many non-infested cells are also uncapped and then recapped which would appear to be a waste of time and energy. In this study we looked at the spatial patterns of recapping and its association with Varroa infestation to understand in what way the uncapping of non-infested cells occurs. We found that recapping occurred in clusters consisting of infested cells and their surrounding non-infested cells. This helped explain our finding that a significant positive correlation existed between levels of recapped infested and non-infested cells. Furthermore, we found that bees responded to an artificial increase in the mite infestation level by increasing their recapping behavior. We confirmed that the recapped area of non-infested cells was significantly smaller, relative to the holes made in the infested cells. Given these findings we propose that recapping behavior is stimulated either by a diffuse signal emanating from the infested cell or that cursory checks are conducted in the vicinity of an infested cell. [ABSTRACT FROM AUTHOR]

Pokkah boeng is an epidemic fungal disease that greatly affects the development of the sugarcane industry in China. Resistance to pokkah boeng disease differs among sugarcane varieties. Breeding and planting resistant cultivars is the most economic and effective measure for controlling this disease. In this study, new sugarcane varieties bred by sugarcane research units in China and the major varieties cultivated in various sugarcane areas, were screened to find new elite pokkah boeng-resistant varieties for application in sugarcane production. Sixty new varieties resistance to pokkah boeng disease were evaluated in regionalized experiments under field conditions. Meanwhile, the resistance of the 31 main cultivated varieties was analyzed under field conditions in Lincang, Puer, and Yuxi, Yunnan Province, and in Yizhou, Guangxi Province, where the incidence of pokkah boeng is particularly high. Field surveys showed that 35 (58.3%) of the 60 new sugarcane varieties were highly resistant to moderately resistant, and 25 (41.7%) were susceptible to highly susceptible. Of the 31 main cultivated varieties, 15 (48.4%) were highly resistant to moderately resistant, and 16 (51.6%) were susceptible to highly susceptible. The results suggested that, in wetter and rainy sugarcane cultivation areas with high incidence of pokkah boeng disease, it is necessary to increase efforts to eliminate the susceptible major cultivated varieties and promote the cultivation of novel resistant varieties. This will help to achieve a reasonable distribution of varieties, fundamentally control the outbreak of pokkah boeng disease, and facilitate the high-quality development of the sugarcane industry in China. [ABSTRACT FROM AUTHOR]

Objective: The research was conducted to study the effect of a complex antimicrobial drug with an anti-inflammatory effect and an antimicrobial drug with an immunostimulating effect on the parameters of nonspecific resistance in calves. Materials and Methods: Two groups (n = 5 each) of sick calves with respiratory pathology were selected for this study. For the treatment of the first experimental group, a complex antimicrobial drug Sulfetrisan® was used. The second experimental group of the calves was intramuscularly injected with the experimental drug gentaaminoseleferon (GIA). To assess the cellular component of immunity in the blood before and after treatment, the number of white blood cells, T-lymphocytes, B-lymphocytes, phagocytic activity of leukocytes, phagocytic number, and phagocytic index (PhI) were determined. In addition, for assessing the humoral component, serum complement activity (SCA), serum lysozyme activity, serum bactericidal activity (SBA), circulating immune complexes (CIC), and total immunoglobulins (total Ig) were measured. The results were compared with the baseline parameters of healthy calves of the control group. Results: When studying the parameters of the humoral and cellular components of nonspecific resistance, it was found that in sick animals, compared with healthy ones, respiratory pathology was accompanied by an imbalance in the immune system. In the process of recovery in animals of the experimental groups under the effect of the drugs, positive changes occurred. However, many of the studied parameters did not reach the values of healthy animals. In the group of calves that received GIA, compared with the calves given Sulfetrisan®, a significant increase in PhI (p < 0.05), SBA (p < 0.006), SCA (p < 0.05), total Ig (p < 0.0005), and CIC (p < 0.05) was observed, which indicated an increase in natural resistance due to the immunostimulating action. Conclusion: The use of GIA in sick animals added to an increase in the general nonspecific cellular and humoral resistance of calves, which made it possible to increase therapeutic efficacy and shorten their recovery time. [ABSTRACT FROM AUTHOR]

Casuarina equisetifolia is an important pioneer tree and suffers from bacterial wilt caused by Ralstonia solanacearum. We collected resistant (R) and susceptible (S) C. equisetifolia clones naturally infected by R. solanacearum and compared their transcriptome and metabolome with a clone (CK) from a non-infested forest, in order to study their response and resistance to bacterial wilt. We identified 18 flavonoids differentially accumulated among the three clonal groups as potential selection biomarkers against R. solanacearum. F lavonoid synthesis-related genes were up-regulated in the resistant clones, probably enhancing accumulation of flavonoids and boosting resistance against bacterial wilt. The down-regulation of auxin/indoleacetic acid-related genes and up-regulation of brassinosteroid, salicylic acid and jasmonic acid-related differentially expressed genes in the R vs CK and R vs S clonal groups may have triggered defense signals and increased expression of defense-related genes against R. solanacearum. Overall, this study provides an important insight into pathogen-response and resistance to bacterial wilt in C. equisetifolia. • Resistant and susceptible clones of Casuarina equisetifolia for Ralstonia solanacearum were identified. • Flavonoid synthesis-related genes were up-regulated in the resistant clones against bacterial wilt. • Down-regulation of auxin/indoleacetic acid-related genes was observed in the resistant clones. • Up-regulation of brassinosteroid, salicylic acid and jasmonic acid-related genes was observed in the resistant clones. • Novel insight into pathogen-response and resistance to bacterial wilt in C. equisetifolia. [ABSTRACT FROM AUTHOR]

Objectives: Probiotics are well documented for their health benefits by developing a balanced intestinal microbiota and boosting immunity. The present study was conducted to determine the effects of a probiotic preparation EnzimsporinTM (consisting of spore-forming bacteria Bacillus subtilis B-2998D, B-3057D, and Bacillus licheniformis B-2999D) on the biochemical, hematological, immunological parameters, intestinal microbiota, and growth dynamics of sheep and lambs. Materials and methods: Enzimsporin was fed to lambs and sheep at different doses to determine the bacteria's probiotic effects. Sheep were divided into three groups (six each), which received 0, 1, and 3 gm of Enzimsporin/per head/day, respectively, and two groups of lambs (10 each), who received 0 gm and 1 gm of Enzimsporin/per head/day for 30 days in addition to their regular ration. On day 30, blood samples were collected, followed by the determination of biochemical, hematological, and natural resistance indicators. Fecal samples were examined to determine the intestinal microflora, and animals were weighed daily to determine their growth dynamics. Results: Supplementation of probiotics (EnzimsporinTM) improved the lambs' body weight gain by 18.8%. Analysis of the clinical parameters showed improvements in the levels of total protein, globulins, and urea by 5.3%, 10.8%, and 6.2%, respectively, in the blood of probiotic-supplemented lambs. Similarly, an increment in the total protein, albumins, and globulins was observed in the sheep with EnzimsporinTM supplementation. The decrease in bilirubin and cholesterol levels in the blood and increased bactericidal and phagocytic index in the sheep and lambs with probiotic supplementation indicated a positive influence of EnzimsporinTM on the liver function and natural resistance. Furthermore, an increase in Lactobacillus and Bifidobacterium and a decrease in the Escherichia coli, Enterococcus, and Yeast in the fecal contents of experimental sheep and lambs indicated the potentiality of EnzimsporinTM on maintaining good gut health. Conclusion: Spore-forming bacteria B. subtilis B-2998D, B-3057D, and B. licheniformis B-2999D can be used in feeding sheep and lambs of 2 months of age to increase body weight gain, improve intestinal microbiota, strengthen the immune system, and maintain normal metabolic processes. [ABSTRACT FROM AUTHOR]

The article presents the materials obtained in the study of the effect of additional fertilizing on the digestive processes and the natural resistance of the body of Karakul sheep with year-round pasture content. We found that sheep and goats that grazed on the pasture for a quarter of a year, regardless of their physiological state, are on half-starved rations, especially in the second half of suyagnost and at the beginning of the spring period. During these periods, the sheep of both groups compared in the winter period daily received from 0.70 to 0.78 energy feed units and from 20 to 28 g of digested protein, and at the beginning of the lactation period, these indicators were 86% of the norm, i.e., they received from 13 to 17% of nutrients. In this regard, we conducted experiments to study the effect of a household diet consisting of coarse feed: 130 g of mixed feed, 50-50% of barley and straw cutting and pasture feed. The sheep of the experimental group received an almost balanced diet for all nutrients. According to the data obtained, the composition and nutritional value of the diet consumed by sheep of the experimental group of feed practically meets their need completely, and in animals of the control group-only 82-85% of the nutritional needs. The highest concentration of total nitrogen was in the rumen of the experimental group - 123.33 mg%, while in the Queens of the control group, its concentration averaged 110.0 mg % or was 12.1% less. In the control group of Queens with a higher consumption of pasture feed, the digestibility of the main nutrients was lower compared to the animals of the experimental group. Inadequate feeding negatively affects the severity of the level of natural resistance. [ABSTRACT FROM AUTHOR]

Respiratory diseases of animals are a significant problem in veterinary medicine. They cause a lot of economic damage to farms, consisting of decreasing productivity and breeding qualities of animals and their deaths. The significant spread of respiratory diseases in animals is caused by decreasing natural resistance due to violation of the housing technology, as well as a high concentration of opportunistic and pathogenic microflora in the indoor air. The results of individual therapy in calves with nonspecific catarrhal bronchopneumonia with the use of the drug Calfmin, which is based on compounds of biogenic micro- and macroelements, and plant immunomodulators, are presented. The clinical parameters of calves, namely, habitus, condition of hair, skin, visible mucous membranes, superficial lymph nodes, body temperature, number of respiratory movements, and heart contractions were studied during bronchopneumonia and their changes under individual treatment of animals. The indicators of protein metabolism in the blood serum of clinically healthy and calves with nonspecific bronchopneumonia were also studied. The content of total protein, albumin, а-, в- and Y-globulins was examined in the blood serum of calves by nephelometric method using phosphate buffers. The dynamics of the comparative analysis of total protein content and its fractions in the blood serum between calves of separate experimental groups is shown. The results of studies indicate a decrease in the body's natural resistance and suppression of immunity in sick calves and the rapid recovery under the conditions of the application of nanoaquachelate macronutrients in the form of the drug Calfmin in combination with Echinacea. [ABSTRACT FROM AUTHOR]

This evaluated the natural resistance of wood from seven Eucalyptus trees in field decay and soil bed tests. Two 12-year-old trees were randomly sampled per species, with 2,2 m logs being obtained from the basal section of each tree. The samples were taken in two positions in the radial direction of the stem (middle heartwood and transition zone; containing heartwood and sapwood). The field decay tests were installed in three municipalities in the southern state of Espírito Santo, and the soil utilized soil from the three field decay test areas. The field decay tests were evaluated after six, 12 and 18 months after installation and the soil bed tests after six months. The Scott-Knott test (p ≤ 0,05) was used in the analysis and evaluation of the tests. The sapwood-heartwood (transition region) exhibited the greatest mass losses for the field decay and soil bed tests. On average, for the soil bed test the lowest mass losses were observed for the soil of Vargem Alta (5,00 %), with greater mass losses observed for São José do Calçado (7,05 %) and Jerônimo Monteiro (9,90 %). In the field decay test the organisms present in the soil of São José do Calçado and related to the organic matter content Eucalyptus grandis and Eucalyptus saligna more intensely.

IntroductionThe HIV-exposed seronegative (HESN) status is for individuals who remain seronegative despite repeated exposure to HIV. One of the main cohorts within this group is men who have sex with men (MSM). Studies of this cohort have revealed different immunological and genetic mechanisms that can explain the phenomenon of natural HIV resistance. NK cells’ higher effector capacity is related to natural resistance to HIV. Besides, a new population of NK cells with adaptive features was described recently. These cells are increased in some HESN cohorts and appear to be involved in better control of viral replication in primarily HIV-infected subjects. The present study evaluated the role of NK cells in the natural resistance to HIV-1 infection in MSM.MethodologyPhenotypic and functional features were evaluated in NK cells from two groups of MSM, at different risks of HIV infection, according to the number of sexual partners. The production of IFN-γ and β-chemokines was included in the analysis, as well as the cytotoxic capacity and adaptive NK cell frequency. Genetic features, such as HLA and KIR allele frequencies, were also explored.ResultsHigh-risk MSM exhibit an increased frequency of fully mature and CD57+/NKG2Chigh NK cells. These individuals also show higher cytotoxic capacity and IFN-γ production in response to K562 stimuli. NK cells with a CD107a+/IFN-γ+ functional profile were found more frequently and displayed higher IFN-γ production capacity among high-risk MSM than among low-risk MSM. The protective allele HLA-B∗18 was only present in the high-risk MSM group as well as HLA-B∗ 39. The protective phenotype KIR3DL1/S1-HLA-B∗Bw4, in a homozygous state, was particularly abundant in the high-risk population. Notably, some of these functional features were related to higher frequencies of mature and CD57+/NKG2Chigh NK cells, which, in turn, were associated with a higher number of sexual partners.ConclusionThe changes observed in the NK cell compartment can be driven by the magnitude of sexual exposure and immunological challenges of high-risk individuals, which could influence their resistance/susceptibility to HIV infection.

Root-knot nematode (RKN) species are predominant pests of crops, attacking stone fruit crops Prunus spp. under Mediterranean climate conditions worldwide. Natural resistance for rootstock breeding is a control method that is gaining interest as an alternative to the highly toxic nematicides. This review first reports an outline of the root-knot nematodes parasitizing stone fruit crops and the Prunus species and rootstocks. It then describes the main sources of resistance detected among the Prunus germplasm and focuses on the major resistance genes identified and their characteristics (spectrum, durability, histological mechanism, effect of temperature, interaction with other pests and diseases, etc.). In peach, besides the RMia reference gene, the new genes PkMi and Mf, also located on chromosome 2, need to be characterized regarding their spectrum and relationship. The two other Prunus reference genes, Ma from plum (complete spectrum) and RMja from almond (more restricted spectrum), are orthologs that belong to a TIR-NB-LRR (TNL) cluster on chromosome 7. The review finally summarizes the positional cloning of the Ma gene and the characterization of its unique TNL structure, encompassing a five-times repeated post-LRR domain. Deciphering how this structure is functionally involved in Ma’s remarkable biological properties is a real challenge for the future.

Introduction: The HIV-exposed seronegative (HESN) status is for individuals who remain seronegative despite repeated exposure to HIV. One of the main cohorts within this group is men who have sex with men (MSM). Studies of this cohort have revealed different immunological and genetic mechanisms that can explain the phenomenon of natural HIV resistance. NK cells' higher effector capacity is related to natural resistance to HIV. Besides, a new population of NK cells with adaptive features was described recently. These cells are increased in some HESN cohorts and appear to be involved in better control of viral replication in primarily HIV-infected subjects. The present study evaluated the role of NK cells in the natural resistance to HIV-1 infection in MSM. Methodology: Phenotypic and functional features were evaluated in NK cells from two groups of MSM, at different risks of HIV infection, according to the number of sexual partners. The production of IFN-γ and β-chemokines was included in the analysis, as well as the cytotoxic capacity and adaptive NK cell frequency. Genetic features, such as HLA and KIR allele frequencies, were also explored. Results: High-risk MSM exhibit an increased frequency of fully mature and CD57+/NKG2Chigh NK cells. These individuals also show higher cytotoxic capacity and IFN-γ production in response to K562 stimuli. NK cells with a CD107a+/IFN-γ+ functional profile were found more frequently and displayed higher IFN-γ production capacity among high-risk MSM than among low-risk MSM. The protective allele HLA-B∗18 was only present in the high-risk MSM group as well as HLA-B∗ 39. The protective phenotype KIR3DL1/S1-HLA-B∗Bw4 , in a homozygous state, was particularly abundant in the high-risk population. Notably, some of these functional features were related to higher frequencies of mature and CD57+/NKG2Chigh NK cells, which, in turn, were associated with a higher number of sexual partners. Conclusion: The changes observed in the NK cell compartment can be driven by the magnitude of sexual exposure and immunological challenges of high-risk individuals, which could influence their resistance/susceptibility to HIV infection. [ABSTRACT FROM AUTHOR]

Epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs) are an approved first-line therapy against unresectable or advanced non-small cell lung cancer (NSCLC) harboring EGFR gene activating mutations. However, the majority of tumors develop acquired resistance against EGFR-TKIs and some tumors exhibit natural resistance. A number of resistance mechanisms against the latest third-generation EGFR-TKIs have been reported, including tertiary EGFR C797S mutation and several gene alterations activating EGFR or other signaling pathways. The current study aimed to identify the frequency of natural EGFR-TKI resistance in pretreatment NSCLC and to predict the therapeutic effect of EGFR-TKIs. A total of 246 EGFR-TKI-naïve NSCLC patients harboring known EGFR gene mutations were identified. The presence of EGFR C797S and T790M mutations were determined using the peptide nucleic acid-locked nucleic acid PCR clamp method. ERBB2, MET, EGFR, ALK, BRAF, FGFR1, MYC, RET, CCND1, CCND2, CDK4, CDK6, MDM2 and MDM4 gene amplification, which can lead to resistance against any generation EGFR-TKIs, was determined using the multiplex ligation-dependent probe amplification assay. No concurrent C797S mutation with known EGFR mutations were identified. T790M mutation was identified in 12 patients (4.9%). ERBB2 or MET gene amplification was found in some patients (0.0-0.4%). MDM2 gene amplification was associated with tumor recurrence and shorter progression-free survival (PFS) for first- or second-generation EGFR-TKIs. De novo EGFR C797S mutation was not identified. Other resistance mechanisms against EGFR-TKIs were indicated in some patients with EGFR-TKI-naïve NSCLC. MDM2 gene amplification, which can lead to altered cell cycle, was associated with tumor recurrence and shorter PFS in EGFR-TKI therapy. [ABSTRACT FROM AUTHOR]

Sitophilus granarius (L.) is considered to be one of the major pests causing damage to cereal grain stored in silos and granaries. Using traditional methods (synthetic insecticides, mechanical, or physical methods) to control this pest is either ineffective or dangerous to people and nature. It is, therefore, necessary to develop new cultivars of cereals that will be distinguished by a high natural tolerance of the foraging by S. granarius. The aim of this study is expressed in the set research hypothesis, stating that the number of offspring of the grain weevil on stored wheat kernels can depend on the content of fatty acids in the kernels. Thus, the qualitative and quantitative composition of fatty acids was determined in kernels of 10 winter wheat cultivars, and the abundance of the beetle’s offspring generation of S. granarius that developed on the wheat grain, as well as the mass of produced dust and loss in the mass of wheat grain were determined. By applying statistical analyses (GLM, ANOVA, Pearson’s linear correlation coefficient, and analysis of redundancy), the presence and character of the dependence between the determined content of fatty acids in wheat grain and the factors describing the development of S. granarius were established. The research results indicate that fatty acids from the groups C 18:1 and C 20:1 probably play an important role as substances stimulating the increase in the number of the tested pest progeny. In contrast, fatty acids C 15:0, C 16:1, and C 18:3, which were determined in large amounts in the grain of wheat cultivars Speedway, KWS Livius, and Julius, can reduce the number of offspring of pest insect.

During scientific-industrial experiment influence of biopreparation STF 1/56 Tim made on the basis of life culture Enterococcus faecium 1/56 on newborn calves of red steppe bred was studied since 2-day age. Animals of test group take preparation daily with milk at doze of 5 ml/head (up to 15-day age) and 10 mg/head (15.20-day age). Animals of control group do not take a preparation. Total amount of eritrocites, leukocites in Goryaev’s count camera. Leucogram was get in smears stained after Romanovsky-Gimza. To determine the bactericidal activity of blood was performed staging of spontaneous and stimulated recovery test nitro blue tetrazolium (NBT-test). At 15-th day of study there were statistically significant (р < 0.001) differences in number of eosinophils (7.09%) and stab neutrophils (13.3%) in animals of experiment group in comparison with calvel of control group (4.01 and 7.31% respectively). Cell protection factors get active. Phagocytic activity of blood cells in the experiment animal was 16.5% higher in compare with control: 56% versus 49.8% respectively. Simultaneously, a statistically significant (p < 0.001) increase in blood neutrophil bactericidal activity was indicated of 57.09% in the experiment versus 46.43% in the control. Indicator of reserve capacity of bactericidal activity in the experimental group was 6.82% against 4.03% in the controls (p < 0.001). It was established that under the influence of probiotic ALS blood on the 10 and 20 day of use of a biological product was higher in calves of the experimental group and was equal to 56.06% and 57.09% against 42.12% and 46.43% in control, respectively. Conducted research and production experience, and the results obtained show that the use of the drug STF 1/56 TiM alimentary to calves of newborn period with milk has a statistically significant effect on hematologic and immunologic status of the animals.

Fusarium graminearum exhibited natural resistance to a majority of succinate dehydrogenase inhibitor fungicides (SDHIs) and the molecular mechanisms responsible for the natural resistance were still unknown. Succinate dehydrogenase subunit C ( SdhC ) is an essential gene for maintaining succinate-ubiquinone oxidoreductase (SQR) function in fungi. In F. graminearum , a paralog of FgSdhC named as FgSdhC 1 was identified. Based on RNA-Seq and qRT-PCR assay, we found that the expression level of FgSdhC was an inessential gene in normal growth but was sufficient for maintaining SQR function and conferred natural resistance or reduced sensitivity toward SDHIs. Additionally, we found that the standard 1 was very low but upregulated by SDHIs treatment. Based on reverse genetics, we demonstrated that FgSdhC 1 was an inessential gene in normal growth but was sufficient for maintaining SQR function and conferred natural resistance or reduced sensitivity toward SDHIs. Additionally, we found that the standard F. graminearum isolate PH-1 had high sensitivity to a majority of SDHIs. A single nucleotide variation (C to T) in the FgSdhC 1 of isolate PH-1, resulting in a premature termination codon (TAA) replacing the fourth amino acid glutamine (Q), led to the failure of FgSdhC 1 to perform functions of conferring nature resistance. These results established that a dispensable paralogous gene determined SDHIs resistance in natural populations of F. graminearum .

Vitamin D (VitD) may modulate anti-HIV-1 responses modifying the risk to acquire the HIV-1-infection. We performed a nested case-control exploratory study involving 413 individuals; HIV-1-exposed seropositives (cases) and seronegatives (HESN) (controls) from three cohorts: sexually-exposed from Colombia and Italy and parenterally-exposed from Spain. The association and interactions of 139 variants in 9 VitD pathway genes, and in 14 antiviral genes with resistance/susceptibility (R/S) to HIV-1 infection was evaluated. Associations between variants and mRNA levels were also analyzed in the Colombian samples. Variants and haplotypes in genes of VitD and antiviral pathways were associated with R/S, but specific associations were not reproduced in all cohorts. Allelic heterogeneity could explain such inconsistency since the associations found in all cohorts were consistently in the same genes: VDR and RXRA of the VitD pathway genes and in TLR2 and RNASE4. Remarkably, the multi-locus genotypes (interacting variants) observed in genes of VitD and antiviral pathways were present in most HESNs of all cohorts. Finally, HESNs carrying resistance-associated variants had higher levels of VitD in plasma, of VDR mRNA in blood cells, and of ELAFIN and defensins mRNA in the oral mucosa. In conclusion, despite allelic heterogeneity, most likely due to differences in the genetic history of the populations, the associations were locus dependent suggesting that genes of the VitD pathway might act in concert with antiviral genes modulating the resistance phenotype of the HESNs. Although these associations were significant after permutation test, only haplotype results remained statistically significant after Bonferroni test, requiring further replications in larger cohorts and functional analyzes to validate these conclusions. • Genetic variation in Vitamin D pathway might be associated with resistance to HIV-1. • Genetic interactions may occur between Vitamin D and antiviral pathways. • Associations with resistance to HIV-1 infection may be population-dependent. [ABSTRACT FROM AUTHOR]

Natural resistance of eight wood species from Caatinga, an exclusive Brazilian biome, was evaluated according to [ASTM Standard (2005) D 2017-05. Standard test method of accelerated laboratory test of natural decay resistance of woods.]. Samples were exposed to white rot (WR) and brown rot (BR) fungi, namely to Trametes versicolor (WR), Pycnoporus sanguineus (WR), Gloeophyllum trabeum (BR) and Gloeophyllum striatum (BR). Weight loss, specific gravity and extractive content of each wood species were evaluated. Diptychandra aurantiaca, Pterodon abruptus and Terminalia fagifolia were classified as "highly resistant", while T. fagifolia was "resistant" to T. versicolor only. Machaerium acutifolium was resistant to all fungi. Aspidosperma multiflorum was resistant to WR fungi and Combretum glaucocarpum to a BR fungus. The species Pityrocarpa moniliformis and Swartzia psilonema were moderately resistant. [ABSTRACT FROM AUTHOR]

Defensins are immune peptides - HDP (Host Defense Peptides) that present high activity against viruses, bacteria and fungi in mammals, including humans. They are an important element of natural resistance in macroorganisms, when microorganisms couldn't developed this resistance during the evolution. Defensins in vertebrate macroorganisms, have direct and indirect impact on germs, including stimulating the synthesis of immune substances such as cytokines, chemokines and growth factors. [ABSTRACT FROM AUTHOR]

The objective of this study was the evaluation of the addition of silica particles in composite on natural resistance and morphology of wood plastic composite against of white rot fungi (Trametes Versicolor). Wood flour at the ratio of 60% (w/w), polypropylene and 2 per hundred compound (phc) MAPP was mixed. Nano and micro silica at 0, 1, 3 and 5 (phc) were added as filler. The mixing process in internal mixer (HAAKE) was done and test specimens were prepared using injection molding. The test specimens were exposed to Trametes Versicolor fungi for 8, 12 and 16 weeks at 25 oC and 75% humidity according to BS 838:1961. Then the mass loss, long-term water absorption and humidity coefficient diffusion of the samples were measured. The formation of hydrogen bonds between silica and wood flour was determined by Fourier Transform Infrared (FTIR) spectroscopy and the morphology of composite was stydied using scanning electron microscopy (SEM). The result showed that with increasing period of samples exposure to fungi, the mass loss, long-term water absorption and humidity coefficient diffusion in wood plastic composite increased. However at higher silica, decay in the samples was decreased. The effect of silica nanoparticles on natural resistance of wood plastic composite was higher than sample containing silica micro particles. Infrared spectroscopy showed hydrogen bonds between wood flour and silica. The result of scanning electron microscopy showed that with increasing of exposure time to fungi, small and large cracks in composite were created. However increasing silica addition, these cracks were decreased.