Search

Your search keyword '"Negosanti M"' showing total 84 results
Start Over Author "Negosanti M"
84 results on '"Negosanti M"'

1. Diagnosis and management of cutaneous and anogenital lichen sclerosus: Recommendations from the Italian Society of Dermatology (SIDemaSt)

Author

Papini, M, Russo, A, Simonetti, O, Borghi, A, Corazza, M, Piaserico, S, Feliciani, C, Calzavara-Pinton, P, Amendolagine, G, Arisi, M, Babino, G, Bardazzi, F, Belloni Fortina, A, Cac-Cavale, S, Campanati, A, Campione, E, Caposiena, C, Carugno, A, Coati, I, Cinotti, E, D'Antuono, A, Dattola, A, Dika, E, Fai, D, Fusano, M, Galluzzo, M, Ganzetti, G, Gisondi, P, Hansel, K, Lora, V, Maione, V, Mandel, V, Menni, S, Negosanti, M, Neri, I, Offidani, A, Parodi, A, Patrizi, A, Peserico, A, Rossi, M, Rivetti, N, Saccani, E, Stan, T, Stingeni, L, Talamonti, M, Vascellaro, A, Vezzoli, P, Virgili, A, Zanca, A, Papini M., Russo A., Simonetti O., Borghi A., Corazza M., Piaserico S., Feliciani C., Calzavara-Pinton P., Amendolagine G., Arisi M., Babino G., Bardazzi F., Belloni Fortina A., Cac-Cavale S., Campanati A., Campione E., Caposiena C. D., Carugno A., Coati I., Cinotti E., D'Antuono A., Dattola A., Dika E., Fai D., Fusano M., Galluzzo M., Ganzetti G., Gisondi P., Hansel K., Lora V., Maione V., Mandel V. D., Menni S., Negosanti M., Neri I., Offidani A., Parodi A., Patrizi A., Peserico A., Rossi M., Rivetti N., Saccani E., Stan T. R., Stingeni L., Talamonti M., Vascellaro A., Vezzoli P., Virgili A., Zanca A., Papini, M, Russo, A, Simonetti, O, Borghi, A, Corazza, M, Piaserico, S, Feliciani, C, Calzavara-Pinton, P, Amendolagine, G, Arisi, M, Babino, G, Bardazzi, F, Belloni Fortina, A, Cac-Cavale, S, Campanati, A, Campione, E, Caposiena, C, Carugno, A, Coati, I, Cinotti, E, D'Antuono, A, Dattola, A, Dika, E, Fai, D, Fusano, M, Galluzzo, M, Ganzetti, G, Gisondi, P, Hansel, K, Lora, V, Maione, V, Mandel, V, Menni, S, Negosanti, M, Neri, I, Offidani, A, Parodi, A, Patrizi, A, Peserico, A, Rossi, M, Rivetti, N, Saccani, E, Stan, T, Stingeni, L, Talamonti, M, Vascellaro, A, Vezzoli, P, Virgili, A, Zanca, A, Papini M., Russo A., Simonetti O., Borghi A., Corazza M., Piaserico S., Feliciani C., Calzavara-Pinton P., Amendolagine G., Arisi M., Babino G., Bardazzi F., Belloni Fortina A., Cac-Cavale S., Campanati A., Campione E., Caposiena C. D., Carugno A., Coati I., Cinotti E., D'Antuono A., Dattola A., Dika E., Fai D., Fusano M., Galluzzo M., Ganzetti G., Gisondi P., Hansel K., Lora V., Maione V., Mandel V. D., Menni S., Negosanti M., Neri I., Offidani A., Parodi A., Patrizi A., Peserico A., Rossi M., Rivetti N., Saccani E., Stan T. R., Stingeni L., Talamonti M., Vascellaro A., Vezzoli P., Virgili A., and Zanca A.

Abstract

Lichen sclerosus (LS) is a disabling chronic inflammatory disease of skin and genital mucous membrane causing itch, pain, dysuria and restriction of micturition, and significant sexual dysfunction and dyspareunia both in women and men. If left untreated, LS is associated with a high degree of sclerosis and scarring, as well as with an elevated risk of cancer in the genital area. Although a central role of autoimmunity is suggested, the pathogenesis of LS is still not clearly understood and the disease remains difficult to treat. The goals of treatment of LS are to alleviate symptoms and discomfort, prevent anatomical changes and prevent malignant transformation. this guideline has been developed by an Italian group of experts. It summarizes evidence-based and expert- based recommendations. The highest level of evidence favors the use of topical high potency corticosteroids; second- and third-line treatments include topical calcineurin inhibitors and topical retinoids, respectively. Surgical treatment has become the treatment of choice in male genital LS with persistent phimosis not responsive to medical treatment. The aim of this paper is to offer evidence-based and easily applicable recommendations for the management of lS.

Published
2021

2. Diagnosis and management of cutaneous and anogenital lichen sclerosus: Recommendations from the Italian Society of Dermatology (SIDemaSt)

Author

Papini, M., Russo, A., Simonetti, O., Borghi, A., Corazza, M., Piaserico, S., Feliciani, C., Calzavara-Pinton, P., Amendolagine, G., Arisi, M., Babino, G., Bardazzi, F., Belloni Fortina, A., Cac-Cavale, S., Campanati, A., Campione, E., Caposiena, C. D., Carugno, A., Coati, I., Cinotti, E., D'Antuono, A., Dattola, A., Dika, E., Fai, D., Fusano, M., Galluzzo, M., Ganzetti, G., Gisondi, P., Hansel, K., Lora, V., Maione, V., Mandel, V. D., Menni, S., Negosanti, M., Neri, I., Offidani, A., Parodi, A., Patrizi, A., Peserico, A., Rossi, M., Rivetti, N., Saccani, E., Stan, T. R., Stingeni, L., Talamonti, M., Vascellaro, A., Vezzoli, P., Virgili, A., Zanca, A., Papini, M., Russo, A., Simonetti, O., Borghi, A., Corazza, M., Piaserico, S., Feliciani, C., Calzavara-Pinton, P., Amendolagine, G., Arisi, M., Babino, G., Bardazzi, F., Belloni Fortina, A., Caccavale, S., Campanati, A., Campione, E., Caposiena, C. D., Carugno, A., Coati, I., Cinotti, E., D'Antuono, A., Dattola, A., Dika, E., Fai, D., Fusano, M., Galluzzo, M., Ganzetti, G., Gisondi, P., Hansel, K., Lora, V., Maione, V., Mandel, V. D., Menni, S., Negosanti, M., Neri, I., Offidani, A., Parodi, A., Patrizi, A., Peserico, A., Rossi, M., Rivetti, N., Saccani, E., Stan, T. R., Stingeni, L., Talamonti, M., Vascellaro, A., Vezzoli, P., Virgili, A., Zanca, A., Papini, M, Russo, A, Simonetti, O, Borghi, A, Corazza, M, Piaserico, S, Feliciani, C, Calzavara-Pinton, P, Amendolagine, G, Arisi, M, Babino, G, Bardazzi, F, Belloni Fortina, A, Cac-Cavale, S, Campanati, A, Campione, E, Caposiena, C, Carugno, A, Coati, I, Cinotti, E, D'Antuono, A, Dattola, A, Dika, E, Fai, D, Fusano, M, Galluzzo, M, Ganzetti, G, Gisondi, P, Hansel, K, Lora, V, Maione, V, Mandel, V, Menni, S, Negosanti, M, Neri, I, Offidani, A, Parodi, A, Patrizi, A, Peserico, A, Rossi, M, Rivetti, N, Saccani, E, Stan, T, Stingeni, L, Talamonti, M, Vascellaro, A, Vezzoli, P, Virgili, A, Zanca, A, Papini M., Russo A., Simonetti O., Borghi A., Corazza M., Piaserico S., Feliciani C., Calzavara-Pinton P., Amendolagine G., Arisi M., Babino G., Bardazzi F., Belloni Fortina A., Cac-Cavale S., Campanati A., Campione E., Caposiena C.D., Carugno A., Coati I., Cinotti E., D'Antuono A., Dattola A., Dika E., Fai D., Fusano M., Galluzzo M., Ganzetti G., Gisondi P., Hansel K., Lora V., Maione V., Mandel V.D., Menni S., Negosanti M., Neri I., Offidani A., Parodi A., Patrizi A., Peserico A., Rossi M., Rivetti N., Saccani E., Stan T.R., Stingeni L., Talamonti M., Vascellaro A., Vezzoli P., Virgili A., and Zanca A.

Subjects
Male, medicine.medical_specialty, Calcineurin Inhibitors, Socio-culturale, Disease, Dermatology, Lichen sclerosus, Guideline, Settore MED/35, Lichen sclerosus et atrophicu, medicine, Dysuria, Humans, Sex organ, Drug therapy, Lichen sclerosus et atrophicus, Operative surgical procedures, Rare diseases, Vulvar lichen sclerosus, Female, Italy, Mometasone Furoate, Lichen Sclerosus et Atrophicus, Calcineurin Inhibitor, business.industry, Mucous membrane, Evidence-based medicine, medicine.disease, Infectious Diseases, medicine.anatomical_structure, Sexual dysfunction, Operative surgical procedure, Vulvar lichen sclerosu, medicine.symptom, business, Rare disease, Human
Abstract

Lichen sclerosus (LS) is a disabling chronic inflammatory disease of skin and genital mucous membrane causing itch, pain, dysuria and restriction of micturition, and significant sexual dysfunction and dyspareunia both in women and men. If left untreated, LS is associated with a high degree of sclerosis and scarring, as well as with an elevated risk of cancer in the genital area. Although a central role of autoimmunity is suggested, the pathogenesis of LS is still not clearly understood and the disease remains difficult to treat. The goals of treatment of LS are to alleviate symptoms and discomfort, prevent anatomical changes and prevent malignant transformation. This guideline has been developed by an Italian group of experts. It summarizes evidence-based and expert- based recommendations. The highest level of evidence favors the use of topical high potency corticosteroids; second- and third-line treatments include topical calcineurin inhibitors and topical retinoids, respectively. Surgical treatment has become the treatment of choice in male genital LS with persistent phimosis not responsive to medical treatment. The aim of this paper is to offer evidence-based and easily applicable recommendations for the management of LS.

Published
2021

9. Valaciclovir versus aciclovir in patient initiated treatment of recurrent genital herpes: A randomised, double blind clinical trial

Author

Bodsworth, N.J., Crooks, R.J., Borelli, S., Vejlsgaard, G., Paavonen, J., Worm, A.M., Uexkull, N., Esmann, J., Strand, A., Ingamells, A.J., Gibb, A. (A.), Barton, S.E. (Simon), Beylot, C. (C.), Bingham, J. (J.), Bojs, G. (G.), Cheetham, D. (D.), Curless, E. (E.), Czarnetzki, B. (B.), Davies, S. (S.), Eichmann, A. (A.), Goh, B., Goldmeir, D. (D.), Gross, G., Haustein, U.F., Kinghorn, G. (G.), Lauharanta, J., Law, C., Luzzi, G. (G.), McMillan, A. (A.), Meaden, J. (J.), Montemagno, U. (U.), Morel, P., Negosanti, M., Nielsen, J.E. (Jorgen), Nilsen, A., Ong, E-K., Ortonne, J.P., Patel, R., Patten, J., Petzold, D., Rufli, T., Saari, S., Shahmanesh, M., Simpanen, A. (A.), Soltz-Szots, J., Stahl, J.P., Stolz, E. (Ernst), Thelin, I., Uexkull, N. von, Wikstrom, A., Woolley, P., Bodsworth, N.J., Crooks, R.J., Borelli, S., Vejlsgaard, G., Paavonen, J., Worm, A.M., Uexkull, N., Esmann, J., Strand, A., Ingamells, A.J., Gibb, A. (A.), Barton, S.E. (Simon), Beylot, C. (C.), Bingham, J. (J.), Bojs, G. (G.), Cheetham, D. (D.), Curless, E. (E.), Czarnetzki, B. (B.), Davies, S. (S.), Eichmann, A. (A.), Goh, B., Goldmeir, D. (D.), Gross, G., Haustein, U.F., Kinghorn, G. (G.), Lauharanta, J., Law, C., Luzzi, G. (G.), McMillan, A. (A.), Meaden, J. (J.), Montemagno, U. (U.), Morel, P., Negosanti, M., Nielsen, J.E. (Jorgen), Nilsen, A., Ong, E-K., Ortonne, J.P., Patel, R., Patten, J., Petzold, D., Rufli, T., Saari, S., Shahmanesh, M., Simpanen, A. (A.), Soltz-Szots, J., Stahl, J.P., Stolz, E. (Ernst), Thelin, I., Uexkull, N. von, Wikstrom, A., and Woolley, P.

Abstract

Objective: To compare the efficacy and safety of twice daily valaciclovir with five times daily aciclovir in the treatment of an episode of recurrent genital herpes simplex virus (HSV) infection in immunocompetent individuals. Methods: 739 patients with a history of recurrent genital HSV infection received either oral valaciclovir (500 mg twice daily) or aciclovir (200 mg five times daily) for 5 days for treatment of their next recurrent episode in a controlled, randomised, double blind trial. Patients self initiated therapy at the first signs and/or symptoms of the HSV recurrence, then were assessed in clinic on five occasions over 7 days, and twice weekly thereafter until lesions had healed. Safety was evaluated through adverse experience reports and haematology and biochemistry monitoring. Results: No significant differences were detected between valaciclovir and aciclovir for the primary endpoint, the duration of all signs and symptoms which included lesion healing and pain/discomfort. The hazard ratio [95% confidence interval] for valaciclovir v aciclovir was 0 93 [0.79, 1.08]. Lesion healing time was similar in each treatment group (hazard ratio valaciclovir v aciclovir 0.96 [0.80, 1.14]). The odds ratio of valaciclovir v aciclovir in preventing the development of vesicular/ulcerative lesions was 1-08 [0.82, 1.42]. Percentages of patients in whom all HSV cultures were negative were similar in the valaciclovir and aciclovir groups at 59% and 54% respectively; for patients having equal to or more than one positive culture result after treatment initiation, cessation of virus shedding was similarly rapid for the two treatments (hazard ratio 0-98 [0.75, 1 .27]). The safety profiles of valaciclovir and aciclovir were comparable with adverse experiences being infrequent and generally mild. Conclusion: This study has demonstrated that valaciclovir 500 mg twice daily is equivalent in efficacy to aciclovir 200 mg five times daily as episodic treatment of recurrent geni

Published
1997
Full Text
View/download PDF

13. Western immunoblotting with five Treponema pallidum recombinant antigens for serologic diagnosis of syphilis.

Author

Sambri, V, Marangoni, A, Eyer, C, Reichhuber, C, Soutschek, E, Negosanti, M, D'Antuono, A, and Cevenini, R

Abstract

Five immunodominant Treponema pallidum recombinant polypeptides (rTpN47, rTmpA, rTpN37, rTpN17, and rTpN15) were blotted onto strips, and 450 sera (200 from blood donors, 200 from syphilis patients, and 50 potentially cross-reactive) were tested to evaluate the diagnostic performance of recombinant Western blotting (recWB) in comparison with in-house whole-cell lysate antigen-based immunoblotting (wclWB) and T. pallidum hemagglutination (MHA-TP) for the laboratory diagnosis of syphilis. None of the serum specimens from blood donors or from potential cross-reactors gave a positive result when evaluated by recWB, wclWB, or MHA-TP. The evaluation of the immunoglobulin G immune response by recWB in sera from patients with different stages of syphilis showed that rTmpA was the most frequently identified antigen (95%), whereas only 41% of the specimens were reactive to rTpN37. The remaining recombinant polypeptides were recognized as follows: rTpN47, 92.5%; rTpN17, 89.5%; and rTpN15, 67.5%. The agreement between recWB and MHA-TP was 95.0% (100% with sera from patients with latent and late disease), and the concordance between wclWB and MHA-TP was 92.0%. The overall concordance between recWB and wclWB was 97.5% (100% with sera from patients with secondary and late syphilis and 94.6 and 98.6% with sera from patients with primary and latent syphilis, respectively). The overall sensitivity of recWB was 98.8% and the specificity was 97.1% with MHA-TP as the reference method. These values for sensitivity and specificity were slightly superior to those calculated for wclWB (sensitivity, 97.1%, and specificity, 96.1%). With wclWB as the standard test, the sensitivity and specificity of recWB were 98.9 and 99.3%, respectively. These findings suggest that the five recombinant polypeptides used in this study could be used as substitutes for the whole-cell lysate T. pallidum antigens and that this newly developed recWB test is a good, easy-to-use confirmatory method for the detection of syphilis antibodies in serum.

Published
2001
Full Text
View/download PDF

14. Treponema pallidum surface immunofluorescence assay for serologic diagnosis of syphilis.

Author

Marangoni, A, Sambri, V, Storni, E, D'Antuono, A, Negosanti, M, and Cevenini, R

Abstract

A surface immunofluorescence assay (SIFA) using live spirochetes was analyzed and compared with Western blot (WB), fluorescent treponemal antibody absorption (FTA-ABS), microhemagglutination (MHA-TP), and Treponema pallidum immobilization (TPI) assays for detecting serum antibodies to T. pallidum in patients with syphilis, in disease controls, and in healthy subjects. SIFA and WB were 99% sensitive (99 of 100 positive specimens) and specific (140 of 140 negative specimens); FTA-ABS showed a sensitivity and a specificity of 90 and 89% (90 of 100 positive and 125 of 140 negative specimens), respectively. MHA-TP showed a sensitivity of 84% (84 of 100 positive specimens) and a specificity of 98.5% (138 of 140 negative specimens). Finally, TPI had a sensitivity of 52% (52 of 100 positive specimens) and a specificity of 100% (140 of 140 negative specimens). The T. pallidum SIFA was therefore highly specific, showing no equivocal reactivities with control sera, and sensitive. The results suggest the possible use of SIFA as a confirmatory test in the serologic diagnosis of syphilis.

Published
2000

19. Assessment of the 'Amplicor' PCR test in the diagnosis of Chlamydia trachomatis infection

Author

Rumpianesi F, MICHELANGELO LA PLACA, D'Antuono A, Negosanti M, and Pavan G

Subjects
Adult, Male, Adolescent, Evaluation Studies as Topic, Culture Techniques, Humans, Chlamydia trachomatis, Female, Chlamydia Infections, Middle Aged, Polymerase Chain Reaction, Sensitivity and Specificity
Abstract

We compared a commercially available PCR assay (Amplicor, Roche, Switzerland) and tissue culture isolation for the detection of C. trachomatis in urethral and/or endocervical swabs. Of the 200 patients studied (130 men and 70 women) PCR and tissue culture gave concordant results in 199 cases; in one case PCR was positive and culture negative. The Amplicor PCR assay proved fast and sensitive and suitable for routine use in most clinical microbiology laboratories.

22. IgE Serum Levels in Psoriasis

Author

Negosanti, M., primary, Fanti, P.A., additional, Gasponi, A., additional, Orlandi, G., additional, Liverani, L., additional, and Tosti, A., additional

Published
1981
Full Text
View/download PDF

25. Immune response against surface-exposed epitopes of Borrelia: A specific tool for serodiagnosis

Author

F. Massaria, Michelangelo La Placa, Alessandra Moroni, Roberto Cevenini, A Farencena, M. Negosanti, Antonella Marangoni, Vittorio Sambri, Lucia D'Apote, Sambri V., Massaria F., Marangoni A., Farencena A., D'Apote L., Moroni A., Negosanti M., La Placa M., and Cevenini R.

Subjects
Immune system, Borrelia burgdorferi, Borrelia, Lyme disease, Immunology and Allergy, Biology, biology.organism_classification, Epitope, Microbiology
Abstract

Not present

Published
1994
Full Text
View/download PDF

26. Adapting a Vacuum Assisted Closure dressing to challenging wounds: negative pressure treatment for perineal necrotizing fasciitis with rectal prolapse in a newborn affected by acute myeloid leukaemia

Author

Luigi Corvaglia, Tommaso Bianchi, Massimino Negosanti, Giacomo Faldella, Paolo Morselli, Arianna Aceti, Luca Negosanti, Annalisa Patrizi, Francesca Negosanti, Riccardo Cipriani, Rossella Sgarzani, Negosanti L, Aceti A, Bianchi T, Corvaglia L, Negosanti F, Sgarzani R, Morselli PG, Cipriani R, Negosanti M, Patrizi A, and Faldella G.

Subjects
medicine.medical_specialty, Myeloid, Vacuum, medicine.medical_treatment, Dermatology, Perineum, Wound care, Negative-pressure wound therapy, medicine, Humans, Pseudomonas Infections, Fasciitis, Necrotizing, Fasciitis, Wound Healing, Debridement, business.industry, Infant, Newborn, Rectal Prolapse, medicine.disease, Bandages, Surgery, Rectal prolapse, Neonatal infection, Leukemia, Myeloid, Acute, medicine.anatomical_structure, Female, business, Negative-Pressure Wound Therapy
Abstract

We report the case of a newborn with acute myeloid leukaemia, who developed perineal necrotizing fasciitis due to Pseudomonas Aeruginosa, after twenty days of life. Following surgical debridement, she was effectively treated with topical negative pressure therapy (V.A.C.(R) device) with silver foam dressings, this achieved complete closure in thirteen days. Negative pressure therapy should be considered when conventional wound care fails to achieve complete wound closure, even in neonates.

Published
2010

27. Un raro caso di tinea corporis

Author

BERTONCELLI, MARCO, STARACE, MICHELA, INFUSINO, SALVATORE DOMENICO, NEGOSANTI, MASSIMINO, NERI, IRIA, PATRIZI, ANNALISA, Bertoncelli R., Bertoncelli M, Starace M, Infusino S, Negosanti M, Neri I, Patrizi A, and Bertoncelli R

Published
2009

28. Acne inversa treated with Infliximab: different outcome in 2 patients

Author

Luca Negosanti, Ivano Iozzo, Claudio Varotti, Angela Antonucci, Massimino Negosanti, Antonucci A, Negosanti M, Negosanti L, Iozzo I, and Varotti C.

Subjects
medicine.medical_specialty, medicine.drug_class, business.industry, medicine.medical_treatment, Antibiotics, Apocrine, Dermatology, General Medicine, Disease, Acne inversa, medicine.disease, Infliximab, Pathogenesis, Incision and drainage, medicine, Etiology, business, Acne, medicine.drug
Abstract

Sir, Acne inversa, also known as Verneuil’s disease or hidradenitis suppurativa, is a common disorder resulting in a chronic, recurrent and debilitating disease. This condition usually presents with painful, inflamed lesions in the apocrine-gland-bearing areas of the body, most commonly the axillary, inguinal and anogenital areas. The disease usually occurs after puberty and before the age of 40 years, suggesting a hormonal influence on the pathogenesis of the disease. Occlusion of the apocrine duct by a keratinous plug and defects of the follicular epithelium have usually been considered the aetiology of acne inversa. Contributing factors include friction from axillary adiposity, sweat, heat, stress, tight clothing and genetic and hormonal components (1). Acne inversa can be treated with antibiotics, retinoids, corticosteroids, cyclosporine, incision and drainage, local wound care, local excision, radiation and laser therapy. Although there are a wide range of therapies suggested for the treatment of acne inversa, the disease is often resistant and the psychological impact on the patient can be great (2), encompassing social, personal and occupational challenges. We describe here 2 case reports of patients affected by acne inversa resistant to traditional therapies, who were treated with infliximab.

Published
2008

29. Detection of Chlamydia trachomatis by a ligase chain reaction amplification method

Author

Roberto Cevenini, Manuela Donati, M. Negosanti, Antonietta D'Antuono, F Rumpianesi, M. La Placa, Rumpianesi F., Donati M., Negosanti M., D'Antuono A., La Placa M., and Cevenini R.

Subjects
Microbiology (medical), Sexually transmitted disease, DNA, Bacterial, Male, Cervicitis, Chlamydia trachomatis, Dermatology, medicine.disease_cause, Sensitivity and Specificity, Chlamydia Infection, Ligases, Chlamydia trachomati, medicine, Humans, Chlamydiaceae, Ligase chain reaction, Direct fluorescent antibody, Chlamydia, biology, business.industry, Public Health, Environmental and Occupational Health, Chlamydia Infections, biology.organism_classification, medicine.disease, Ligase, Virology, Infectious Diseases, Chlamydiales, Immunology, Female, business, Nucleic Acid Amplification Techniques, Human
Abstract

Background and Objectives: The ligase chain reaction is an in vitro DNA amplification technique that exponentially amplifies selected DNA sequences. Goal: To evaluate a ligase chain reaction assay for the detection of Chlamydia trachomatis cryptic plasmid DNA (LCx Chlamydia) in patients routinely attending a sexually transmitted disease center in Italy. Study Design: Urethral or cervical swabs were obtained from 501 consecutive patients (334 men and 167 women). The samples were assayed in parallel with LCx Chlamydia and conventional tissue culture; discordant results were further assayed by direct immunofluorescence and a ligase chain reaction with alternate primers. Results: After resolution of discordant results, the LCx method showed a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 99.3%, 96.7%, and 100% in men; 100%, 100%, 100%, and 100% in women; and 100%, 99.5%, 97.1%, and 100% overall, respectively. By comparison, the sensitivity of tissue culture was 81.4% in men, 50% in women, and 77.6% overall. Conclusions: The automated LCx method is sensitive, fast, and accurate and represents a useful diagnostic tool for C. trachomatis infection, even in low and medium prevalence populations.

Published
1996

30. Use of the ligase chain reaction on urine of men and their female sexual partners for detection of genital Chlamydia trachomatis infection

Author

Roberto Cevenini, Massinzo Negosanti, Michelangelo La Placa, Manuela Donati, Fabio Rurnpianesi, Antonietta D'Antuono, Rumpianesi F., Donati M., La Placa M., Negosanti M., D'Antuono A., and Cevenini R.

Subjects
Gynecology, Chlamydia trachomatis infection, Microbiology (medical), medicine.medical_specialty, media_common.quotation_subject, Tissue/cell culture, Chlamydia trachomatis, General Medicine, Urine, Biology, medicine.disease, medicine.disease_cause, Urination, Infectious Diseases, Chlamydia trachomati, Immunology, LCR, medicine, Sex organ, Urethritis, Ligase chain reaction, media_common
Abstract

Objective The purpose of the present study was to evaluate an in vitro DNA amplification assay named the ligase chain reaction (LCR) for the detection of Chlamydia trachomatis cryptic plasmid DNA in urine from men and women, in comparison with urethral swab culture in men and cervical swab culture in women. Methods 591 patients (394 men with urethritis and 197 female sex partners) attending a center for sexually transmitted diseases in northern Italy between January 1994 and January 1995 were enrolled in this study. A cervical swab was collected from women and a urethral swab from men for standard tissue cell culture. From each patient 20 mL of the first stream of the urine (FVU), taken at least 2 h after the last urination, were collected for LCR analysis. Discrepant results were further analyzed by direct fluorescence and a LCR with alternative primers. Results In men the prevalence of C. trachomatis infection by urethral culture was 13.45% and, after resolution of discordant results, the LCR method performed on FVU showed a sensitivity, specificity, positive predictive value and negative predictive value of 89.4%, 100%, 100% and 98.2%, respectively; the sensitivity of tissue cell culture was 92.8%. In female sex partners, the prevalence of C. trachomatis infection by cervical culture was 3.04%; LCR detected eight true positive samples, two more than tissue cell culture, and no false-negative results. Conclusion LCR analysis of FVU is a rapid, non-invasive technique and represents a good alternative to tissue cell culture. Further study is needed to investigate possible LCR inhibitors present in urine samples.

Published
1996

32. A long history of widespread asymptomatic giant plaques.

Author

Chessa MA, Sacchelli L, Misciali C, Negosanti M, Dika E, and Patrizi A

Subjects
Aged, Biopsy, Diagnosis, Differential, Erythema pathology, Exanthema pathology, Granuloma pathology, Humans, Male, Sarcoidosis pathology, Skin pathology, Erythema diagnosis, Exanthema diagnosis, Granuloma diagnosis, Sarcoidosis diagnosis
Published
2018
Full Text
View/download PDF

35. Erythematous patches and pretibial ulcers: an uncommon presentation of cutaneous sarcoidosis.

Author

Barisani A, Negosanti M, La Placa M, Infusino SD, Misciali C, Sorci M, and Patrizi A

Subjects
Diagnosis, Differential, Erythema therapy, Female, Humans, Leg Ulcer therapy, Middle Aged, Sarcoidosis therapy, Treatment Outcome, Erythema diagnosis, Erythema etiology, Leg Ulcer diagnosis, Leg Ulcer etiology, Sarcoidosis complications, Sarcoidosis diagnosis
Published
2016
Full Text
View/download PDF

36. Adapting a Vacuum Assisted Closure dressing to challenging wounds: negative pressure treatment for perineal necrotizing fasciitis with rectal prolapse in a newborn affected by acute myeloid leukaemia.

Author

Negosanti L, Aceti A, Bianchi T, Corvaglia L, Negosanti F, Sgarzani R, Morselli PG, Cipriani R, Negosanti M, Patrizi A, and Faldella G

Subjects
Bandages, Debridement, Fasciitis, Necrotizing microbiology, Female, Humans, Infant, Newborn, Pseudomonas Infections microbiology, Rectal Prolapse microbiology, Vacuum, Wound Healing, Fasciitis, Necrotizing therapy, Leukemia, Myeloid, Acute complications, Negative-Pressure Wound Therapy methods, Perineum, Pseudomonas Infections therapy, Rectal Prolapse therapy
Abstract

We report the case of a newborn with acute myeloid leukaemia, who developed perineal necrotizing fasciitis due to Pseudomonas Aeruginosa, after twenty days of life. Following surgical debridement, she was effectively treated with topical negative pressure therapy (V.A.C.(R) device) with silver foam dressings, this achieved complete closure in thirteen days. Negative pressure therapy should be considered when conventional wound care fails to achieve complete wound closure, even in neonates.

Published
2010
Full Text
View/download PDF

38. Treatment of refractory pemphigus vulgaris with anti-CD20 monoclonal antibody (rituximab): five cases.

Author

Antonucci A, Negosanti M, Tabanelli M, and Varotti C

Subjects
Adult, Antibodies, Monoclonal administration & dosage, Antibodies, Monoclonal, Murine-Derived, Drug Administration Schedule, Female, Humans, Infusions, Intravenous, Male, Pemphigus pathology, Recurrence, Rituximab, Severity of Illness Index, Antibodies, Monoclonal therapeutic use, Antigens, CD20, Pemphigus drug therapy
Abstract

Background: Pemphigus vulgaris is an autoimmune disease characterized by blisters and widespread erosions, involving skin and mucous membranes, caused by autoantibodies to desmoglein 1 and 3. This pathology is associated with increased morbidity and mortality if untreated. The treatment of pemphigus vulgaris requires multiple immunosuppressive agents, but often it is particularly resistant., Objective: To evaluate the efficacy and safety of rituximab therapy in refractory pemphigus vulgaris., Methods: Five patients diagnosed as having pemphigus vulgaris were treated with anti-CD20 monoclonal antibody (rituximab). Each patient was treated with rituximab intravenously at a dosage of 375 mg per square metre of body surface area once weekly for 4 weeks., Results: All the patients presented clinical resolution. No adverse effects were observed. It is important to observe the clinical evolution in the future, but our experience is still limited to a short lifetime and follow-up., Conclusion: In our experience rituximab has been an effective and safe treatment for refractory pemphigus vulgaris.

Published
2007
Full Text
View/download PDF

39. Western immunoblotting with five Treponema pallidum recombinant antigens for serologic diagnosis of syphilis.

Author

Sambri V, Marangoni A, Eyer C, Reichhuber C, Soutschek E, Negosanti M, D'Antuono A, and Cevenini R

Subjects
Antigens, Bacterial genetics, Blotting, Western methods, Humans, Recombinant Proteins genetics, Recombinant Proteins immunology, Serologic Tests, Syphilis diagnosis, Antigens, Bacterial immunology, Syphilis immunology, Treponema pallidum immunology
Abstract

Five immunodominant Treponema pallidum recombinant polypeptides (rTpN47, rTmpA, rTpN37, rTpN17, and rTpN15) were blotted onto strips, and 450 sera (200 from blood donors, 200 from syphilis patients, and 50 potentially cross-reactive) were tested to evaluate the diagnostic performance of recombinant Western blotting (recWB) in comparison with in-house whole-cell lysate antigen-based immunoblotting (wclWB) and T. pallidum hemagglutination (MHA-TP) for the laboratory diagnosis of syphilis. None of the serum specimens from blood donors or from potential cross-reactors gave a positive result when evaluated by recWB, wclWB, or MHA-TP. The evaluation of the immunoglobulin G immune response by recWB in sera from patients with different stages of syphilis showed that rTmpA was the most frequently identified antigen (95%), whereas only 41% of the specimens were reactive to rTpN37. The remaining recombinant polypeptides were recognized as follows: rTpN47, 92.5%; rTpN17, 89.5%; and rTpN15, 67.5%. The agreement between recWB and MHA-TP was 95.0% (100% with sera from patients with latent and late disease), and the concordance between wclWB and MHA-TP was 92.0%. The overall concordance between recWB and wclWB was 97.5% (100% with sera from patients with secondary and late syphilis and 94.6 and 98.6% with sera from patients with primary and latent syphilis, respectively). The overall sensitivity of recWB was 98.8% and the specificity was 97.1% with MHA-TP as the reference method. These values for sensitivity and specificity were slightly superior to those calculated for wclWB (sensitivity, 97.1%, and specificity, 96.1%). With wclWB as the standard test, the sensitivity and specificity of recWB were 98.9 and 99.3%, respectively. These findings suggest that the five recombinant polypeptides used in this study could be used as substitutes for the whole-cell lysate T. pallidum antigens and that this newly developed recWB test is a good, easy-to-use confirmatory method for the detection of syphilis antibodies in serum.

Published
2001
Full Text
View/download PDF

40. Treponema pallidum surface immunofluorescence assay for serologic diagnosis of syphilis.

Author

Marangoni A, Sambri V, Storni E, D'Antuono A, Negosanti M, and Cevenini R

Subjects
Adult, Aged, Antibody Specificity, Antigens, Bacterial analysis, Antigens, Bacterial immunology, Antigens, Surface immunology, Blotting, Western, Female, Fluorescent Antibody Technique, Humans, Male, Middle Aged, Sensitivity and Specificity, Serologic Tests, Syphilis immunology, Treponema Immobilization Test, Treponema pallidum immunology, Antigens, Surface analysis, Syphilis diagnosis, Treponema pallidum isolation & purification
Abstract

A surface immunofluorescence assay (SIFA) using live spirochetes was analyzed and compared with Western blot (WB), fluorescent treponemal antibody absorption (FTA-ABS), microhemagglutination (MHA-TP), and Treponema pallidum immobilization (TPI) assays for detecting serum antibodies to T. pallidum in patients with syphilis, in disease controls, and in healthy subjects. SIFA and WB were 99% sensitive (99 of 100 positive specimens) and specific (140 of 140 negative specimens); FTA-ABS showed a sensitivity and a specificity of 90 and 89% (90 of 100 positive and 125 of 140 negative specimens), respectively. MHA-TP showed a sensitivity of 84% (84 of 100 positive specimens) and a specificity of 98.5% (138 of 140 negative specimens). Finally, TPI had a sensitivity of 52% (52 of 100 positive specimens) and a specificity of 100% (140 of 140 negative specimens). The T. pallidum SIFA was therefore highly specific, showing no equivocal reactivities with control sera, and sensitive. The results suggest the possible use of SIFA as a confirmatory test in the serologic diagnosis of syphilis.

Published
2000
Full Text
View/download PDF

42. IgG western blot as a confirmatory test in early syphilis.

Author

Marangoni A, Sambri V, Olmo A, D'Antuono A, Negosanti M, and Cevenini R

Subjects
Adult, Animals, Female, Fluorescent Antibody Technique, Hemagglutination Tests, Humans, Male, Middle Aged, Rabbits, Sensitivity and Specificity, Syphilis immunology, Syphilis Serodiagnosis, Treponema pallidum immunology, Antibodies, Bacterial immunology, Blotting, Western methods, Immunoglobulin G immunology, Syphilis diagnosis
Abstract

Sensitivity and specificity of IgG detection by Western blotting performed with a lysate of Treponema pallidum whole cells were compared with those of the most common assays used in the laboratory diagnosis of syphilis, i.e. fluorescent treponemal antibody absorption test (FTA-ABS) and treponemal haemagglutination assay (TPHA). Thirty-five serum samples obtained from twenty-one patients with a clinical diagnosis of early syphilis, based on the presence of typical chancre or skin or mucous membrane lesions, were studied. In addition, thirty blood samples from donors, ten sera positive for Borrelia burgdorferi and five positive for Leptospira interrogans were tested as controls. The clinical diagnosis was the reference method used to compare the performance of the serological tests. Western blotting performed with a sensitivity and specificity of 100%, whereas the corresponding sensitivity and specificity for FTA-ABS were 88.5% and 98%, respectively. The performance of TPHA showed a sensitivity of 86% and a specificity of 100%.

Published
1999
Full Text
View/download PDF

44. Detection of Chlamydia trachomatis by a ligase chain reaction amplification method.

Author

Rumpianesi F, Donati M, Negosanti M, D'Antuono A, La Placa M, and Cevenini R

Subjects
DNA, Bacterial, Female, Humans, Ligases, Male, Sensitivity and Specificity, Chlamydia Infections microbiology, Chlamydia trachomatis isolation & purification, Nucleic Acid Amplification Techniques
Abstract

Background and Objectives: The ligase chain reaction is an in vitro DNA amplification technique that exponentially amplifies selected DNA sequences., Goal: To evaluate a ligase chain reaction assay for the detection of Chlamydia trachomatis cryptic plasmid DNA (LCx Chlamydia) in patients routinely attending a sexually transmitted disease center in Italy., Study Design: Urethral or cervical swabs were obtained from 501 consecutive patients (334 men and 167 women). The samples were assayed in parallel with LCx Chlamydia and conventional tissue culture; discordant results were further assayed by direct immunofluorescence and a ligase chain reaction with alternate primers., Results: After resolution of discordant results, the LCx method showed a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 99.3%, 96.7%, and 100% in men; 100%, 100%, 100%, and 100% in women; and 100%, 99.5%, 97.1%, and 100% overall, respectively. By comparison, the sensitivity of tissue culture was 81.4% in men, 50% in women, and 77.6% overall., Conclusions: The automated LCx method is sensitive, fast, and accurate and represents a useful diagnostic tool for C. trachomatis infection, even in low and medium prevalence populations.

Published
1996
Full Text
View/download PDF

45. Use of the ligase chain reaction on urine of men and their female sexual partners for detection of genital Chlamydia trachomatis infection.

Author

Rumpianesi F, Donati M, La Placa M, Negosanti M, D'Antuono A, and Cevenini R

Abstract

OBJECTIVE: The purpose of the present study was to evaluate an in vitro DNA amplification assay named the ligase chain reaction (LCR) for the detection of Chlamydia trachomatis cryptic plasmid DNA in urine from men and women, in comparison with urethral swab culture in men and cervical swab culture in women. METHODS: 591 patients (394 men with urethritis and 197 female sex partners) attending a center for sexually transmitted diseases in northern Italy between January 1994 and January 1995 were enrolled in this study. A cervical swab was collected from women and a urethral swab from men for standard tissue cell culture. From each patient 20 mL of the first stream of the urine (FVU), taken at least 2 h after the last urination, were collected for LCR analysis. Discrepant results were further analyzed by direct fluorescence and a LCR with alternative primers. RESULTS: In men the prevalence of C. trachomatis infection by urethral culture was 13.45% and, after resolution of discordant results, the LCR method performed on FVU showed a sensitivity, specificity, positive predictive value and negative predictive value of 89.4%, 100%, 100% and 98.2%, respectively; the sensitivity of tissue cell culture was 92.8%. In female sex partners, the prevalence of C. trachomatis infection by cervical culture was 3.04%; LCR detected eight true positive samples, two more than tissue cell culture, and no false-negative results. CONCLUSION: LCR analysis of FVU is a rapid, non-invasive technique and represents a good alternative to tissue cell culture. Further study is needed to investigate possible LCR inhibitors present in urine samples.

Published
1996
Full Text
View/download PDF

46. Assessment of the "Amplicor" PCR test in the diagnosis of Chlamydia trachomatis infection.

Author

Rumpianesi F, La Placa M Jr, D'Antuono A, Negosanti M, and Pavan G

Subjects
Adolescent, Adult, Chlamydia Infections microbiology, Chlamydia trachomatis genetics, Culture Techniques, Evaluation Studies as Topic, Female, Humans, Male, Middle Aged, Sensitivity and Specificity, Chlamydia Infections diagnosis, Chlamydia trachomatis isolation & purification, Polymerase Chain Reaction methods
Abstract

We compared a commercially available PCR assay (Amplicor, Roche, Switzerland) and tissue culture isolation for the detection of C. trachomatis in urethral and/or endocervical swabs. Of the 200 patients studied (130 men and 70 women) PCR and tissue culture gave concordant results in 199 cases; in one case PCR was positive and culture negative. The Amplicor PCR assay proved fast and sensitive and suitable for routine use in most clinical microbiology laboratories.

Published
1993

47. [Tungiasis. A clinical case].

Author

D'Antuono A, Gatti M, Negosanti M, Passarini B, Pauluzzi P, and Reggiani M

Subjects
Adult, Animals, Humans, Male, Ectoparasitic Infestations parasitology, Foot Dermatoses parasitology, Siphonaptera
Abstract

Tungiasis is a cutaneous infestation by the female sand flea, Tunga penetrans. Because of the increase in international travel, the disease is reported in Europe, in spite of it being formerly restricted to the equatorial zones. This report describes a case of tungiasis and discusses clinical features, diagnosis and treatment.

Published
1990

48. [Incidence of onychomycoses in ungual pathology].

Author

De Padova MP, Negosanti M, D'Antuono A, and Pizzino D

Subjects
Adolescent, Adult, Aged, Female, Humans, Italy, Male, Middle Aged, Onychomycosis microbiology, Onychomycosis epidemiology
Published
1988

50. [Thymopentin in the treatment of recurrent condylomata acuminata].

Author

Ghetti P, Negosanti M, Andriani GC, Beltrandi E, and Maldini M

Subjects
Adjuvants, Immunologic administration & dosage, Adolescent, Adult, Anus Neoplasms blood, Anus Neoplasms immunology, Condylomata Acuminata blood, Condylomata Acuminata immunology, Female, Genital Neoplasms, Female blood, Genital Neoplasms, Female immunology, Humans, Injections, Subcutaneous, Male, Middle Aged, Peptide Fragments administration & dosage, Recurrence, Thymopentin, Thymopoietins administration & dosage, Adjuvants, Immunologic therapeutic use, Anus Neoplasms drug therapy, Condylomata Acuminata drug therapy, Genital Neoplasms, Female drug therapy, Peptide Fragments therapeutic use, Thymopoietins therapeutic use, Thymus Hormones therapeutic use
Abstract

Twelve patients who had been suffering from genital and/or perianal recurrent condyloma acuminatum for a minimum of 5 to a maximum of 24 months, in spite of treatment, were studied from the immunological viewpoint and treated with 50 mg s.c. Thymopentin three times a week for 4 or 6 weeks. Six of the patients were cured at the end of treatment, five after 5 months, and one was not cured. Analysis of the clinico-laboratory data shows a significant agreement between the course of clinical signs and the immunological picture. The various cure stages are probably attributable to the basic immune arrangement which was more impaired in the 5 patients who were cured more slowly and in the non-cured case. In the latter too, however, Thymopentin permitted correcting the balance of the relationship between the various lymphocyte subpopulations.

Published
1989

Catalog

Books, media, physical & digital resources
See catalog results