38 results on '"Nemcova J"'
Search Results
2. Renal angiomyoadenomatous tumor: morphologic, immunohistochemical, and molecular genetic study of a distinct entity
- Author
-
Michal, M., Hes, O., Nemcova, J., Sima, R., Kuroda, N., Bulimbasic, S., Franco, M., Sakaida, N., Danis, D., Kazakov, D. V., Ohe, C., and Hora, M.
- Published
- 2009
- Full Text
- View/download PDF
3. EP265 The role of methylation silencing of tumor suppressor genes in cervical HSIL: a prospective cytologic-histologic correlation study of 70 cases
- Author
-
Ondic, O, primary, Bouda, J, additional, Nemcova, J, additional, Kinkorova-Lunackova, I, additional, Chytra, J, additional, Presl, J, additional, Alaghehbandan, R, additional, Cerna, K, additional, Gomolcakova, B, additional, Kostun, J, additional, Sidlova, H, additional, Vlasak, P, additional, and Majek, O, additional
- Published
- 2019
- Full Text
- View/download PDF
4. Significance of transcriptionally-active high-risk human papillomavirus in sinonasal squamous cell carcinoma: Case series and a meta-analysis.
- Author
-
SVAJDLER, M., NEMCOVA, J., DUBINSKY, P., METELKOVA, A., SVAJDLER, P., STRAKA, L., SAKAR, R., DAUM, O., MICHAL, M., SKALOVA, A., and MEZENCEV, R.
- Subjects
META-analysis ,SQUAMOUS cell carcinoma ,HUMAN papillomavirus vaccines ,DNA ,IMMUNOHISTOCHEMISTRY ,POLYMERASE chain reaction - Abstract
Sinonasal cancers represent a highly heterogeneous group of head and neck cancers, for which etiological and prognostic significance of high-risk human papillomavirus (HPV) infections has not yet been conclusively established. We investigated the presence of transcriptionally-active high-risk HPV in a series of 34 sinonasal squamous cell cancer (SNSCC) cases and evaluated the effect of transcriptionally-active HPV on the overall survival. In addition, we performed a meta-analysis of previously published studies, including this study, to summarize the prevalence of HPV positivity across histological subtypes of SNSCC. The presence of transcriptionally-active HPV was detected by HPV mRNA using the polymerase chain reaction (PCR) or in situ hybridization (ISH). p16 expression was evaluated as a surrogate marker for transcriptionallyactive HPV infection by immunohistochemistry (IHC), the presence of high-risk HPV DNA was tested by PCR and the HPV genotypes were determined by sequencing of PCR amplicons. Transcriptionally-active HPV infections were found in ~25% of the SNSCC cases. The role of HPV infection in keratinizing SNSCC may be higher than previously reported (~32% in our study vs. ~0-6.3% in all other studies). Patients with transcriptionally-active HPV-positive SNSCCs were more likely to be diagnosed at earlier stages (p<0.05) and displayed better mean overall survival, although the difference between HPV-positive and HPV-negative groups was not statistically significant. In contrast to other non-oropharyngeal squamous cell carcinomas (non-OPSCCs) of the head and neck, in SNSCCs, p16/IHC and p16/IHC+HPV DNA displayed high specificity as surrogate markers of transcriptionally-active HPV infections. However, p16/IHC may have significantly lower sensitivity as a surrogate marker of transcriptionally-active HPV in SNSCCs compared to OPSCCs. Furthermore, in our group of SNSCCs, all cases positive for high-risk HPV DNA by PCR were also transcriptionally-active (causative) infections with positive HPV mRNA by ISH. Our results imply a possible different role of HPV-mediated carcinogenesis of squamous cell epithelium in oropharyngeal and sinonasal sites with the latter displaying a lower proportion of causative HPV infections; nevertheless, most cases positive for high-risk HPV DNA, p16/IHC or combination thereof were also found positive for transcriptionally-active HPV. The prognostic significance of HPV status in SNSCCs remains inconclusive and future studies should investigate the presence of transcriptionally-active HPV by direct HPV testing. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
5. Using Water Jet in Orthopaedic Surgery
- Author
-
Hloch, S., Foldyna, J., Kľoc, J., Magurová, D., Kozak, D., Ruggiero, A., Tozan, H., Monka, P., Monková, K., Hvizdoš, P., Chattopadyaya S., Zeleňák, M., Nemcova J., Živić, Marija, and Galeta, Tomislav
- Subjects
water jet ,bone cement ,bone ,orthopedic ,nursing - Abstract
Paper refers to possibilities of the water jet technology utilization during (re)implantations of total endoprostheses of large joints aimed at minimizing the bone losses and thermo-mechanical damage to the bone tissue. Outlined are main problems and potential improvement by application of progressive technique water jet. Paper summarizes up-to-date research of the team. Reason of the research is to develop surgical technique, without heat and mechanical damage of large joints, with minimal traumatizing effects on the patient and a maximum yield for the society.
- Published
- 2012
6. Mucinous tubular and spindle cell carcinoma with Fuhrman nuclear grade 3, A histological, immunohistochemical, ultrastructural and FISH study
- Author
-
Kuroda, Naoto, Hes, O., Michal, Michal, Nemcova, J., Gal, V., Yamaguchi, T., Kawada, T., Imamura, Y., Hayashi, Yoshihiro, and Lee, Gang-Hong
- Subjects
616.6 - Patología del sistema genitourinario ,Kidney ,Renal cell carcinoma - Abstract
Mucinous tubular and spindle cell carcinoma (MTSCC) of the kidney generally shows low nuclear grade. MTSCC with high nuclear grade is relatively rare. In this article, we report two cases of MTSCC with Fuhrman grade 3. One case occurred in a 57-year-old Japanese female and the second case in a 49-year-old Caucasian female. Histologically, the tumors were composed of neoplastic cells with cuboidal or columnar and spindle morphology, and Fuhrman nuclear grade 3. The myxoid stroma was also observed. This stroma was positive for Alcian blue stain. Immunohistochemically, neoplastic cells of both cases were positive for AMACR, but negative for CD10 and RCC Ma. Ultrastructurally, tumorous cells of one case contained numerous mitochondria. In FISH analysis, many neoplastic cells of both cases demonstrated monosomy of chromosomes 15 and 22 and disomy of chromosomes 7 and 17. One of the two patients died of respiratory failure due to pleuritis carcinomatosa 48 months postoperatively. Finally, the pathologist should recognize that high grade MTSCC exists despite its rare frequency. FISH analysis may be helpful in establishing the diagnosis of this entity. Furthermore, we present the first report of a patient with MTSCC dying of distant metastasis.
- Published
- 2008
7. MUSEUM AND GALLERY EDUCATION AND ITS APLICATION IN THE CONTEXT OF PRE-PRIMARY EDUCATION
- Author
-
Hrubá Miriama, Kačírek Ľuboš, Nemcová Jana, Osaďan Róbert, and Szentesiová Lenka
- Subjects
MUSEUM ,CHILDREN’S MUSEUM ,PRE-PRIMARY EDUCATION CURRICULUM ,MUSEUM PRACTICE ,Museums. Collectors and collecting ,AM1-501 - Abstract
The presented article deals with the issue of cooperation among museum and gallery institutions and pre-primary education. Its content is structured into a theoretical and a research part that are closely connected. In the theoretical part, there is an analysis of the curriculum of pre-primary education and an analysis of the possibility of a cooperation among the pre-primary schools and the museum institutions with regard to the (educational) needs and interests of the child visitor. This connection is then specified in the chapter devoted to the phenomenon of the children’s museum, set especially in the European context. In the theoretical part, we also checked the offer of Slovak museums and galleries intended for pre-school children (performances, workshops, interactive exhibitions). The research part of the article, based on interviews with teachers and directors of nursery schools, examines not only the possibilities of cooperation between the pre-primary school and museums and galleries, but also the specific needs and requirements of pre-primary practice towards these institutions in order to fulfill their educational potential to the fullest possible extent.
- Published
- 2019
8. Renal angiomyoadenomatous tumor: morphologic, immunohistochemical, and molecular genetic study of a distinct entity
- Author
-
Michal, M., primary, Hes, O., additional, Nemcova, J., additional, Sima, R., additional, Kuroda, N., additional, Bulimbasic, S., additional, Franco, M., additional, Sakaida, N., additional, Danis, D., additional, Kazakov, D. V., additional, Ohe, C., additional, and Hora, M., additional
- Published
- 2008
- Full Text
- View/download PDF
9. PREEMPTIVE VALGANCICLOVIR THERAPY IS NOT ASSOCIATED WITH INCREASE IN CYTOMEGALOVIRUS (CMV) DNA IN RENAL ALLOGRAFT BIOPSY SPECIMENS COMPARED WITH VALACYCLOVIR PROPHYLAXIS
- Author
-
Reischig, T, primary, Nemcova, J, additional, Vanecek, T, additional, Jindra, P, additional, Hes, O, additional, Bouda, M, additional, and Treska, V, additional
- Published
- 2008
- Full Text
- View/download PDF
10. Realization theory of Nash systems.
- Author
-
Nemcova, J., Petreczky, M., and van Schuppen, J.H.
- Published
- 2009
- Full Text
- View/download PDF
11. Monoclonal antibody to human chronic myeloid leukemia cell line MOLM-7 specifically reacts with an antigen of apoptotic cells
- Author
-
Hradcova, M., Marinov, I., Novak, J. T., Nemcova, J., and Stockbauer, P.
- Published
- 2002
- Full Text
- View/download PDF
12. Our Experience with MRI Monitoring of Multiple Sclerosis Patients in Clinical Practice
- Author
-
Vaneckova, M., Seidl, Z., Krasensky, J., Horakova, D., Havrdova, E., Nemcova, J., and Danes, J.
13. Retrospective Study of Magnetic Resonance Imaging of the Brain and Spine in Neuromyelitis Optica
- Author
-
Vaneckova, M., Horakova, D., Havrdova, E., Petra Nytrova, Nemcova, J., and Seidl, Z.
14. Human Papillomavirus (HPV) in breast tumors: prevalence in a group of Mexican patients
- Author
-
Cetina Lucely, Villavicencio Verónica, Turcios Elmer, Mykyskova Iva, Nemcova Jana, Montiel Delia, de León David, Coronel Alberto, and Hes Ondraj
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Breast cancer is one of the main health problems in developed countries, occupying first place in mortality in women. It is well-known that there are risk factors associated with breast cancer development. Nonetheless, in 50–80% of cases known risk factors have not been identified, this has generated the attempt to identify new factors related with this neoplasia as viral infections. The aim of this work is investigate the prevalence of HPV DNA in patients with breast lesions at the Instituto Nacional de Cancerologia de Mexico. Methods Fifty-one cases of breast cancer were selected from the files of the institute and compared by age and tumor size with 43 cases of non malignant breast lesions (fibroadenoma, fibrocystic disease and phyllodes tumor). Paraffin embedded specimens were selected, HPV DNA was analyzed by polymerase chain reaction (PCR) and sequenced for different types of HPV in case of positivity for HPV-DNA. Descriptive analysis of clinical and pathological variables was performed and comparisons between positive and negative cases was done. Results All patients were mexican, mean age was 53.3, median age of menarche was 13 and median tumor size 9 cms. Cervicovaginal cytology was performed to all patients, 1 patient (1.9%) of cancer group had HPV and none in the other group, no cases were diagnosed with cervical dysplasia. In the group of carcinomas 36 (70.5%) were negative and 15 (29.4%) were positive to HPV-DNA, 10(66.6%) were positive for HPV 16, 3(20%) for HPV 18, two cases (13.4%) were positive for both. In the group of benign conditions all were negative to HPV-DNA. Conclusion Presence of HPV in breast cancer in our group of cases is high in comparison to other authors; larger numbers of cases need to be analyzed in order to establish the exact role of this virus in the pathogenesis of breast cancer.
- Published
- 2009
- Full Text
- View/download PDF
15. A Randomized Trial of Valganciclovir Prophylaxis Versus Preemptive Therapy in Kidney Transplant Recipients.
- Author
-
Reischig T, Vlas T, Kacer M, Pivovarcikova K, Lysak D, Nemcova J, Drenko P, Machova J, Bouda M, Sedivcova M, and Kormunda S
- Subjects
- Humans, Valganciclovir adverse effects, Antiviral Agents adverse effects, Cytomegalovirus genetics, Transplant Recipients, Kidney Transplantation adverse effects, Cytomegalovirus Infections epidemiology, Neutropenia chemically induced, Neutropenia complications
- Abstract
Significance Statement: Although cytomegalovirus (CMV) infection is an important factor in the pathogenesis of kidney allograft rejection, previous studies have not determined the optimal CMV prevention strategy to avoid indirect effects of the virus. In this randomized trial involving 140 kidney transplant recipients, incidence of acute rejection at 12 months was not lower with valganciclovir prophylaxis (for at least 3 months) compared with preemptive therapy initiated after detection of CMV DNA in whole blood. However, prophylaxis was associated with a lower risk of subclinical rejection at 3 months. Although both regimens were effective in preventing CMV disease, the incidence of CMV DNAemia (including episodes with higher viral loads) was significantly higher with preemptive therapy. Further research with long-term follow-up is warranted to better compare the two approaches., Background: The optimal regimen for preventing cytomegalovirus (CMV) infection in kidney transplant recipients, primarily in reducing indirect CMV effects, has not been defined., Methods: This open-label, single-center, randomized clinical trial of valganciclovir prophylaxis versus preemptive therapy included kidney transplant recipients recruited between June 2013 and May 2018. After excluding CMV-seronegative recipients with transplants from seronegative donors, we randomized 140 participants 1:1 to receive valganciclovir prophylaxis (900 mg, daily for 3 or 6 months for CMV-seronegative recipients who received a kidney from a CMV-seropositive donor) or preemptive therapy (valganciclovir, 900 mg, twice daily) that was initiated after detection of CMV DNA in whole blood (≥1000 IU/ml) and stopped after two consecutive negative tests (preemptive therapy patients received weekly CMV PCR tests for 4 months). The primary outcome was the incidence of biopsy-confirmed acute rejection at 12 months. Key secondary outcomes included subclinical rejection, CMV disease and DNAemia, and neutropenia., Results: The incidence of acute rejection was lower with valganciclovir prophylaxis than with preemptive therapy (13%, 9/70 versus 23%, 16/70), but the difference was not statistically significant. Subclinical rejection at 3 months was lower in the prophylaxis group (13% versus 29%, P = 0.027). Both regimens prevented CMV disease (in 4% of patients in both groups). Compared with prophylaxis, preemptive therapy resulted in significantly higher rates of CMV DNAemia (44% versus 75%, P < 0.001) and a higher proportion of patients experiencing episodes with higher viral load (≥2000 IU/ml), but significantly lower valganciclovir exposure and neutropenia., Conclusion: Among kidney transplant recipients, the use of valganciclovir prophylaxis did not result in a significantly lower incidence of acute rejection compared with the use of preemptive therapy., Clinical Trial Registry Name and Registration Number: Optimizing Valganciclovir Efficacy in Renal Transplantation (OVERT Study), ACTRN12613000554763 ., (Copyright © 2023 by the American Society of Nephrology.)
- Published
- 2023
- Full Text
- View/download PDF
16. Prevalence of oral and anal human papillomavirus infection in Czech predominantly HIV-positive men having sex with men - data from a previously unreported population.
- Author
-
Nemcova J, Riegert J, Cerna K, Rob F, Smahelova J, Hercogova JT, Martinek P, and Ondic O
- Subjects
- Male, Humans, Homosexuality, Male, Prevalence, Cross-Sectional Studies, Czech Republic epidemiology, Risk Factors, Papillomaviridae genetics, Anal Canal, Papillomavirus Infections diagnosis, HIV Infections complications, HIV Infections epidemiology, HIV Infections diagnosis, Sexual and Gender Minorities, HIV Seropositivity epidemiology
- Abstract
Background: HIV-positive men who have sex with men (MSM) are more likely to experience human papillomavirus (HPV) infection. The persistent HPV infection is the major factor in the development of anal and oropharyngeal neoplasms. Data on the prevalence of anal and oral HPV in MSM are almost absent from the countries of Central and Eastern Europe. We conducted a cross-sectional study focused on the prevalence of oral and anal HPV infections and the relationship between current anal and oral HPV intrapersonal infection in a Czech population of predominantly HIV-positive MSM., Methods: Oral gargle and anal swab samples from 205 predominantly HIV-positive MSM from the Czech Republic were analysed for HPV infection using PCR. Selected sociodemographic and clinical data were correlated with HPV detection using generalized linear models and multivariate analysis., Results: HPV infection was detected in 183 (96.8%) anal and 48 (23.6%) oral samples. The most common type of HR-HPV was HPV16 in both anal (25.4%) and oral (2.5%) samples. Multiple anal HPV infections and the presence of vaccine-targeted HR-HPV types were significantly correlated with abnormal anal cytology and HIV status., Conclusion: The prevalence of anal HPV infection in Czech predominantly HIV-positive MSM ranks among the highest reported, while oral HPV prevalence is consistent with MSM populations. Minimal overlap of oral and anal HPV types within a patient was observed.
- Published
- 2022
- Full Text
- View/download PDF
17. Prevalence of high-risk human papillomavirus DNA and mRNA and its association with abnormal anal cytology in the Czech male anal cancer screening cohort.
- Author
-
Nemcova J, Cerna K, Rob F, Smahelova J, Tresnak Hercogova J, Marx J, Traxmandlova I, and Ondic O
- Subjects
- Adult, Anus Neoplasms genetics, Czech Republic, Early Detection of Cancer statistics & numerical data, Female, Homosexuality, Male statistics & numerical data, Humans, Male, Middle Aged, Papillomavirus Infections pathology, Papillomavirus Infections virology, Prevalence, Sexual and Gender Minorities statistics & numerical data, Young Adult, Anus Neoplasms pathology, DNA, Viral genetics, Homosexuality, Male genetics, Papillomavirus Infections epidemiology, RNA, Messenger genetics
- Abstract
Background: Anal cancer (AC) screening is justified in high-risk populations, particularly HIV-positive men having sex with men (MSM). HR-HPV testing could improve the efficiency of cytologically based screening of AC, as in the screening of biologically analogical cervical cancer. The specificity of HR-HPV testing is influenced by the prevalence of HR-HPV infection in the screened population. Reported anal HR-HPV DNA prevalence in MSM is high, but HR-HPV mRNA reflects rather long-term infections and is more specific for high-grade lesions. However, no data were published about HR-HPV DNA and mRNA prevalence in the Czech AC screening population., Method: Results of liquid-based anal cytology of 203 predominantly HIV-positive MSM from the Czech AC screening cohort were correlated with results of DNA and E6/E7 mRNA testing of 14 HR-HPV types, and HPV16 genotyping. Eighty-one MSM underwent a standard anoscopy., Results: A total of 109 (53.7%) samples had abnormal cytology, with 12 (5.9%) ASC-H/HSIL, 67 (33.0%) samples cytologically negative, and 27 (13.3%) unsatisfactory. HR-HPV DNA was detected in 134 (66.0%) and HR-HPV RNA in 72 (35.5%) anal smears. HR-HPV mRNA and HPV16 mRNA positivity were associated with abnormal cytology (p = .0037, p = .0021). No significant association was found between HR-HPV DNA or HPV16 DNA positivity and abnormal cytology. No high-grade lesions were revealed by anoscopy., Conclusion: Prevalence of anal HR-HPV DNA among Czech MSM is high, however, the prevalence of HR-HPV mRNA is half and associated with abnormal cytology. Our results indicate an increased efficiency of cytological screening when combined with HR-HPV mRNA testing., (© 2021 Wiley Periodicals LLC.)
- Published
- 2021
- Full Text
- View/download PDF
18. BK polyomavirus and valganciclovir: Highly suspected association urgently calling for a new randomized trial.
- Author
-
Reischig T, Kacer M, Hes O, Machova J, Nemcova J, Lysak D, Jindra P, Pivovarcikova K, Kormunda S, and Bouda M
- Subjects
- Cytomegalovirus, Ganciclovir, Humans, Valganciclovir, Viremia, BK Virus, Cytomegalovirus Infections
- Published
- 2019
- Full Text
- View/download PDF
19. Viral load and duration of BK polyomavirus viraemia determine renal graft fibrosis progression: histologic evaluation of late protocol biopsies.
- Author
-
Reischig T, Kacer M, Hes O, Machova J, Nemcova J, Kormunda S, Pivovarcikova K, and Bouda M
- Subjects
- BK Virus isolation & purification, BK Virus pathogenicity, Disease Progression, Female, Fibrosis etiology, Graft Survival, Humans, Kidney Diseases etiology, Male, Middle Aged, Polyomavirus Infections virology, Prospective Studies, Transplantation, Homologous, Tumor Virus Infections virology, Viremia virology, Virus Replication, Fibrosis pathology, Kidney Diseases pathology, Kidney Transplantation adverse effects, Polyomavirus Infections complications, Tumor Virus Infections complications, Viral Load, Viremia complications
- Abstract
Background: Polyomavirus BK (BKV) infection of the renal allograft causes destructive tissue injury with inflammation and subsequent fibrosis., Methods: Using a prospective cohort of patients after kidney transplantation performed between 2003 and 2012, we investigated the role of BKV viraemia in the development and progression of interstitial fibrosis and tubular atrophy (IFTA). The primary outcome was moderate-to-severe IFTA assessed by protocol biopsy at 36 months., Results: A total of 207 consecutive recipients were enrolled. Of these, 57 (28%) developed BKV viraemia with 10 (5%) cases of polyomavirus-associated nephropathy (PVAN). Transient (<3 months) BKV viraemia occurred in 70% of patients, and persistent (≥3 months) BKV viraemia in 30%. A high viral load (≥10 000 copies/mL) was detected in 18% and a low viral load (<10 000 copies/mL) in 61%, while the viral load could not be determined in 21%. Moderate-to-severe IFTA was significantly increased in high [71%; odds ratio (OR) = 12.1; 95% confidence interval (CI) 1.62-90.0; P = 0.015] or persistent BKV viraemia (67%; OR = 6.33; 95% CI 1.19-33.7; P = 0.031) with corresponding rise in 'interstitial fibrosis + tubular atrophy' scores. Only patients with transient low BKV viraemia showed similar incidence and progression of IFTA to the no-BKV group. Persistent low BKV viraemia was uncommon yet the progression of fibrosis was significant. Only recipients with PVAN experienced inferior graft survival at 5 years., Conclusions: These data suggest that only transient low BKV viraemia does not negatively affect the progression of allograft fibrosis in contrast to excessive risk of severe fibrosis after high or persistent BKV viraemia., (© The Author(s) 2019. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
20. Cytomegalovirus prevention strategies and the risk of BK polyomavirus viremia and nephropathy.
- Author
-
Reischig T, Kacer M, Hes O, Machova J, Nemcova J, Lysak D, Jindra P, Pivovarcikova K, Kormunda S, and Bouda M
- Subjects
- Adult, BK Virus, Cytomegalovirus, Female, Graft Survival, Humans, Kidney Failure, Chronic complications, Male, Middle Aged, Multivariate Analysis, Premedication, Proportional Hazards Models, Prospective Studies, Randomized Controlled Trials as Topic, Risk Factors, Treatment Outcome, Valacyclovir therapeutic use, Valganciclovir therapeutic use, Cytomegalovirus Infections prevention & control, Kidney Failure, Chronic surgery, Kidney Transplantation adverse effects, Polyomavirus Infections virology, Tumor Virus Infections prevention & control, Viremia etiology
- Abstract
Polyomavirus BK (BKV) is the cause of polyomavirus-associated nephropathy resulting in premature graft loss. There are limited data regarding the role of cytomegalovirus (CMV) infection and its prevention in developing BKV viremia and PVAN. In a prospective study, we analyzed 207 consecutive renal transplant recipients previously enrolled in 2 randomized trials evaluating different CMV prevention regimens with routine screening for BKV and CMV. Of these, 59 received valganciclovir and 100 valacyclovir prophylaxis; 48 patients were managed by preemptive therapy. At 3 years, the incidence of BKV viremia and PVAN was 28% and 5%, respectively. CMV DNAemia developed in 55% and CMV disease in 6%. Both BKV viremia (42% vs 23% vs 21%, P = .006) and PVAN (12% vs 2% vs 2%, P = .011) were increased in patients treated with valganciclovir prophylaxis compared to valacyclovir and preemptive therapy. Using multivariate Cox proportional hazard regression, valganciclovir prophylaxis was independent predictor of BKV viremia (hazard ratio [HR] = 2.38, P = .002) and PVAN (HR = 4.73, P = .026). In contrast, the risk of subsequent BKV viremia was lower in patients with antecedent CMV DNAemia (HR = 0.50, P = .018). These data suggest valganciclovir prophylaxis may be associated with increased risk of BKV viremia and PVAN. CMV DNAemia did not represent a risk for BKV., (© 2019 The American Society of Transplantation and the American Society of Transplant Surgeons.)
- Published
- 2019
- Full Text
- View/download PDF
21. Syringocystadenoma Papilliferum of the Anogenital Area and Buttocks: A Report of 16 Cases, Including Human Papillomavirus Analysis and HRAS and BRAF V600 Mutation Studies.
- Author
-
Konstantinova AM, Kyrpychova L, Nemcova J, Sedivcova M, Bisceglia M, Kutzner H, Zamecnik M, Sehnalkova E, Pavlovsky M, Zateckova K, Shvernik S, Spurkova Z, Michal M, Kerl K, and Kazakov DV
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Anal Canal pathology, Buttocks pathology, Female, Genital Neoplasms, Female genetics, Genital Neoplasms, Female pathology, Genital Neoplasms, Female virology, Genital Neoplasms, Male genetics, Genital Neoplasms, Male pathology, Humans, Male, Middle Aged, Mutation, Papillomaviridae, Sweat Gland Neoplasms pathology, Sweat Gland Neoplasms virology, Tubular Sweat Gland Adenomas virology, Young Adult, Papillomavirus Infections epidemiology, Proto-Oncogene Proteins B-raf genetics, Proto-Oncogene Proteins p21(ras) genetics, Sweat Gland Neoplasms genetics, Tubular Sweat Gland Adenomas genetics, Tubular Sweat Gland Adenomas pathology
- Abstract
Syringocystadenoma papilliferum (SCAP) is a benign tumor most commonly located on the head and neck area often associated with nevus sebaceus. In its usual location, the human papillomavirus (HPV) DNA and mutations in the RAS/mitogen-activated protein kinase signaling pathway have been detected in SCAP. We studied 16 cases of SCAP in the anogenital areas and buttock where this neoplasm is rare and attempted to find out whether SCAP in these sites have different histopathological and molecular biological features. It seems that there is no significant difference between the morphology of anogenital SCAP and SCAP in other locations. Several tumors in our cohort demonstrated features resembling those seen in warts, but HPV DNA was not found in these lesions. On the contrary, we identified DNA of HPV high-risk types in some tumors without HPV-related morphology. Our study confirms the role of HRAS and BRAF V600 mutations in the pathogenesis of SCAP, including SCAP in the anogenital areas and buttock.
- Published
- 2019
- Full Text
- View/download PDF
22. Cribriform adenocarcinoma of minor salivary gland origin principally affecting the tongue: characterization of new entity.
- Author
-
Skalova A, Sima R, Kaspirkova-Nemcova J, Simpson RH, Elmberger G, Leivo I, Di Palma S, Jirasek T, Gnepp DR, Weinreb I, Perez-Ordoñez B, Mukensnabl P, Rychly B, Hrabal P, and Michal M
- Subjects
- Adenocarcinoma genetics, Adenocarcinoma mortality, Adenocarcinoma radiotherapy, Adenocarcinoma secondary, Adenocarcinoma surgery, Adenocarcinoma virology, Adult, Aged, Aged, 80 and over, Biopsy, DNA Mutational Analysis, DNA, Viral isolation & purification, Female, Humans, Immunohistochemistry, Lymphatic Metastasis, Male, Middle Aged, Neck Dissection, Neoplasm Recurrence, Local, Papillomaviridae genetics, Radiotherapy, Adjuvant, Registries, Retrospective Studies, Salivary Gland Neoplasms genetics, Salivary Gland Neoplasms mortality, Salivary Gland Neoplasms radiotherapy, Salivary Gland Neoplasms surgery, Salivary Gland Neoplasms virology, Salivary Glands, Minor surgery, Salivary Glands, Minor virology, Time Factors, Tongue Neoplasms genetics, Tongue Neoplasms mortality, Tongue Neoplasms radiotherapy, Tongue Neoplasms surgery, Tongue Neoplasms virology, Treatment Outcome, Adenocarcinoma pathology, Salivary Gland Neoplasms pathology, Salivary Glands, Minor pathology, Tongue Neoplasms pathology
- Abstract
We present a series of 23 cases of a distinctive, hitherto poorly recognized low-grade adenocarcinoma, with several histologic features reminiscent of papillary carcinoma of the thyroid, and which mostly but not exclusively occurs in the tongue. All the tumors were unencapsulated and were divided into lobules that were composed mainly of cribriform and solid growth patterns. Therefore, we propose the name "cribriform adenocarcinoma of minor salivary gland origin (CAMSG)." All the patients were adults with a mean age at diagnosis of 55.8 years (range, 25 to 85 y). Fourteen of the 23 tumors were localized in the tongue, 3 in the soft palate, 2 in the retromolar buccal mucosa, 3 in the lingual tonsils, and 1 in the upper lip. Fifteen patients of 23 had synchronous metastases in the cervical lymph nodes at the time of diagnosis, bilateral in 3 cases. In 3 patients, the nodal metastasis was the first evidence of disease, later investigation revealing primary neoplasms in the base of tongue and tonsil, respectively. In addition, 1 patient developed a cervical lymph node metastasis 8 years after excision of a primary tumor of the tongue. Data on treatment and follow-up were available in 14 cases. The patients were treated by radical excision with clear margins (12 cases) or by simple excision (2 cases). Neck dissection was performed in 10 patients; 9 received radiotherapy, but none were treated by chemotherapy. Clinical follow-up ranged from 2 months to 13 years (mean, 6 y and 5 mo). Twelve patients are alive with no evidence of recurrent or metastatic disease after treatment, 1 patient died 2 years after surgery without evidence of tumor, and 1 patient is alive with recurrent tumor of the palate.
- Published
- 2011
- Full Text
- View/download PDF
23. Multiple (familial) trichoepitheliomas: a clinicopathological and molecular biological study, including CYLD and PTCH gene analysis, of a series of 16 patients.
- Author
-
Kazakov DV, Vanecek T, Zelger B, Carlson JA, Spagnolo DV, Schaller J, Nemcova J, Kacerovska D, Vazmitel M, Sangüeza M, Emberger M, Belousova I, Fernandez-Figueras MT, Kempf W, Meyer DR, Rütten A, Baltaci M, and Michal M
- Subjects
- Adolescent, Adult, Carcinoma, Skin Appendage pathology, Child, DNA Mutational Analysis, Deubiquitinating Enzyme CYLD, Female, Gene Expression, Gene Expression Profiling, Humans, Male, Middle Aged, Mutation, Neoplastic Syndromes, Hereditary genetics, Neoplastic Syndromes, Hereditary pathology, Patched Receptors, Patched-1 Receptor, Polymerase Chain Reaction, Skin Neoplasms pathology, Young Adult, Carcinoma, Skin Appendage genetics, Receptors, Cell Surface genetics, Skin Neoplasms genetics, Tumor Suppressor Proteins genetics
- Abstract
Multiple familial trichoepitheliomas (MFT) constitute an autosomally inherited syndrome possibly related to Brooke-Spiegler syndrome (BSS). Although some early studies suggested a role for the PTCH gene on chromosome 9q22.3 in the etiopathogenesis of MFT, recent studies of occasional patients with the MFT clinical phenotype identified mutations in the CYLD gene on chromosome 16q12-q13, a gene responsible for BSS. A systematic investigation of PTCH and CYLD mutations in patients with MFT has never been performed. Our main objective was to collect a reasonably large series of patients with MFT to (1) study the clinicopathological spectrum of the disease, (2) determine whether the PTCH gene is implicated in the pathogenesis of MFT, and if so (3) determine the relative frequency of CYLD and PTCH mutations, (4) establish if there may be any possible genotype-phenotype correlations, and (5) study the spectrum of somatic mutations. Clinical analysis including family histories, histopathological investigations, and molecular genetic studies were performed. There were 9 female and 7 male patients ranging in age from 11 to 63 years. They presented with multiple, small, discrete and sometimes confluent, skin-colored to pink, asymptomatic nodules preferentially located on the face, being especially prominent and confluent in the nasolabial folds and inner aspects of the eyebrows. A total of 66 conventional trichoepitheliomas (TEs) were studied microscopically. Aside from typical features of TE, some also exhibited variant morphological patterns including areas reminiscent of other benign adnexal neoplasms and melanocytic hyperplasia. In none of the 9 patients tested was a germline mutation of the PTCH gene identified. Germline CYLD mutations were detected in 6 of 13 patients tested (identical in 2 unrelated patients) including 2 novel mutations, whereas the remaining 7 individuals showed wild-type alleles. Two patients with germline wild-type CYLD showed, however, a somatic mutation in the gene (1 duplication, 1 substitution mutation). Neither CYLD nor PTCH germline mutations were found in the 5 patients in whom both genes were analyzed. MFT seems to be a phenotypic variant of BSS. The PTCH gene is rarely, if ever, involved in the pathogenesis of MFT. Absence of a germline mutation of the CYLD gene in cases harboring a somatic mutation may be explained by large deletions in the gene or by mutation in intronic sequences or in the promoter region. Considering our 5 patients with no mutation in either gene, the final possibility is that another, as yet undescribed gene (neither CYLD nor PTCH) is implicated in the pathogenesis of some patients with MFT.
- Published
- 2011
- Full Text
- View/download PDF
24. Pancreatic undifferentiated carcinoma with osteoclast-like giant cells masquerading as (extra)gastrointestinal stromal tumor: potential diagnostic pitfall.
- Author
-
Daum O, Ferdova E, Kural T, Grossmann P, Nemcova J, Mukensnabl P, and Michal M
- Subjects
- Aged, Diagnosis, Differential, Female, Giant Cells pathology, Humans, Osteoclasts pathology, Adenocarcinoma pathology, Gastrointestinal Stromal Tumors pathology, Pancreatic Neoplasms pathology
- Published
- 2010
- Full Text
- View/download PDF
25. Spectrum of tumors with follicular differentiation in a patient with the clinical phenotype of multiple familial trichoepitheliomas: a clinicopathological and molecular biological study, including analysis of the CYLD and PTCH genes.
- Author
-
Kazakov DV, Vanecek T, Nemcova J, Kacerovska D, Spagnolo DV, Mukensnabl P, and Michal M
- Subjects
- Adolescent, Adult, Cell Differentiation, Child, Deubiquitinating Enzyme CYLD, Female, Humans, Male, Neoplasms, Adnexal and Skin Appendage genetics, Neoplasms, Basal Cell genetics, Neoplasms, Multiple Primary genetics, Patched Receptors, Patched-1 Receptor, Pedigree, Phenotype, Skin Neoplasms genetics, Neoplasms, Adnexal and Skin Appendage pathology, Neoplasms, Basal Cell pathology, Neoplasms, Multiple Primary pathology, Receptors, Cell Surface genetics, Skin Neoplasms pathology, Tumor Suppressor Proteins genetics
- Abstract
We report a patient with multiple trichoepitheliomas whose biopsy material also demonstrated a range of other neoplasms with follicular differentiation, including small nodular trichoblastoma, small nodular basal cell carcinoma (BCC), and areas resembling infundibulocystic BCC/basaloid follicular hamartoma. These were all intimately associated with otherwise typical trichoepitheliomas that dominated the microscopic appearances. Peripheral blood and tumor tissues of the patient and his 2 daughters, who apparently had a milder phenotype, were studied for alterations in the CYLD and PTCH genes, but mutations or loss of heterozygosity was not found in either gene. The occurrence of multiple follicular neoplasms within a single lesion adds evidence that, although in most cases BCC and trichoblastoma are distinct lesions, the 2 neoplasms do encompass a morphological spectrum of follicular differentiation, which is probably more overtly expressed in syndromic patients.
- Published
- 2009
- Full Text
- View/download PDF
26. Acinic cell carcinoma with high-grade transformation: a report of 9 cases with immunohistochemical study and analysis of TP53 and HER-2/neu genes.
- Author
-
Skálová A, Sima R, Vanecek T, Muller S, Korabecna M, Nemcova J, Elmberger G, Leivo I, Passador-Santos F, Walter J, Rousarova M, Jedlickova K, Curik R, Geierova M, and Michal M
- Subjects
- Adult, Aged, Biomarkers, Tumor analysis, Carcinoma, Acinar Cell metabolism, Cell Transformation, Neoplastic genetics, DNA Mutational Analysis, Female, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Male, Middle Aged, Receptor, ErbB-2 genetics, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Carcinoma, Acinar Cell genetics, Carcinoma, Acinar Cell pathology, Genes, erbB-2, Genes, p53, Parotid Neoplasms genetics, Parotid Neoplasms pathology
- Abstract
High-grade transformation of acinic cell carcinoma (AciCC) (previously referred to as dedifferentiation) is a rare phenomenon characterized by histologic progression of low-grade AciCC to high-grade adenocarcinoma or undifferentiated carcinoma. We report 9 new cases with immunohistochemical analysis and examination of HER-2/neu and p53 genes to further define the profile of this tumor. Histologically, the high-grade component was composed of polymorphic cells with a high mitotic rate arranged in glandular and solid growth patterns with comedonecrosis. The MIB-1 labeling indices were elevated in the high-grade component, as compared with the low grade conventional AciCC. The high-grade component of AciCC was characterized by strong membrane staining for CK18 and beta-catenin, and nuclear staining for cyclin-D1. HER-2/neu, androgen receptor, C-kit, and epidermal growth factor receptor were absent from both low-grade and high-grade components. In contrast, S-100 protein, alpha-1-antitrypsin, and lysozyme were lost only in high-grade foci of transformed AciCC. The median age was 61 years (with range from 43 to 76 y). Lymph node (LN) metastases were found in 5 of 9 cases (56%). Distant metastases to the lungs (n=4), pleura (n=2), brain (n=3), and peritoneum (n=1), and paraaortic, paratracheal, and mediastinal LNs (n=2) were observed. Six of 9 patients (66%) died from tumor dissemination, all with a median overall survival of 4.3 years (range: 1 to 9 y). The high propensity for LN metastases indicates the need for neck dissection at the time of diagnosis.
- Published
- 2009
- Full Text
- View/download PDF
27. Crystal-deficient alveolar soft-part sarcoma with cutaneous involvement: a case report.
- Author
-
Kacerovska D, Michal M, Nemcova J, Vanecek T, Kreuzberg B, Mrazkova P, Koudela K Jr, and Kazakov DV
- Subjects
- Angiography methods, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors genetics, Humans, Immunohistochemistry, Intracellular Signaling Peptides and Proteins, Leg, Lung Neoplasms secondary, Male, Middle Aged, Neoplasm Recurrence, Local, Oncogene Proteins, Fusion genetics, Positron-Emission Tomography, Sarcoma, Alveolar Soft Part blood supply, Sarcoma, Alveolar Soft Part genetics, Sarcoma, Alveolar Soft Part surgery, Sequence Analysis, DNA, Soft Tissue Neoplasms blood supply, Soft Tissue Neoplasms genetics, Soft Tissue Neoplasms surgery, Tomography, X-Ray Computed, Treatment Outcome, Cytoplasmic Granules pathology, Sarcoma, Alveolar Soft Part pathology, Skin pathology, Soft Tissue Neoplasms pathology
- Abstract
Alveolar soft-part sarcoma (ASPS) is a rare distinctive sarcoma, in most cases involving deep soft tissues of the extremities. It is associated with a specific unbalanced translocation, der(17)t(X;17)(p11;q25) that results in the formation of an ASPL-TFE3 fusion gene. Microscopically, it is typified by an alveolar growth of large cells containing typical periodic acid-Schiff-positive rod-shaped crystals, often serving as a diagnostic clue. Other distinctive features include nuclear immunoreactivity for transcription factor 3 (TFE3) protein and a typical ultrastructural finding of large crystals with a rectangular or rhomboid shape. The authors present an unusual case of ASPS with cutaneous involvement, which did not exhibit typical large crystals; there were striking round granules. Molecular genetic study revealed fusion transcript ASPL-TFE3, type 2. To the best of our knowledge, cutaneous involvement of a crystal-deficient ASPS has not been reported.
- Published
- 2009
- Full Text
- View/download PDF
28. Human papillomavirus (HPV) in breast tumors: prevalence in a group of Mexican patients.
- Author
-
de León DC, Montiel DP, Nemcova J, Mykyskova I, Turcios E, Villavicencio V, Cetina L, Coronel A, and Hes O
- Subjects
- Adult, Aged, Aged, 80 and over, DNA, Viral isolation & purification, Female, Humans, Mexico, Middle Aged, Papillomaviridae genetics, Retrospective Studies, Breast Neoplasms virology, Papillomaviridae isolation & purification, Papillomavirus Infections diagnosis
- Abstract
Background: Breast cancer is one of the main health problems in developed countries, occupying first place in mortality in women. It is well-known that there are risk factors associated with breast cancer development. Nonetheless, in 50-80% of cases known risk factors have not been identified, this has generated the attempt to identify new factors related with this neoplasia as viral infections. The aim of this work is investigate the prevalence of HPV DNA in patients with breast lesions at the Instituto Nacional de Cancerologia de Mexico., Methods: Fifty-one cases of breast cancer were selected from the files of the institute and compared by age and tumor size with 43 cases of non malignant breast lesions (fibroadenoma, fibrocystic disease and phyllodes tumor). Paraffin embedded specimens were selected, HPV DNA was analyzed by polymerase chain reaction (PCR) and sequenced for different types of HPV in case of positivity for HPV-DNA. Descriptive analysis of clinical and pathological variables was performed and comparisons between positive and negative cases was done., Results: All patients were mexican, mean age was 53.3, median age of menarche was 13 and median tumor size 9 cms. Cervicovaginal cytology was performed to all patients, 1 patient (1.9%) of cancer group had HPV and none in the other group, no cases were diagnosed with cervical dysplasia. In the group of carcinomas 36 (70.5%) were negative and 15 (29.4%) were positive to HPV-DNA, 10(66.6%) were positive for HPV 16, 3(20%) for HPV 18, two cases (13.4%) were positive for both. In the group of benign conditions all were negative to HPV-DNA., Conclusion: Presence of HPV in breast cancer in our group of cases is high in comparison to other authors; larger numbers of cases need to be analyzed in order to establish the exact role of this virus in the pathogenesis of breast cancer.
- Published
- 2009
- Full Text
- View/download PDF
29. Liver tuberculoma.
- Author
-
Treska V, Hes O, and Nemcova J
- Subjects
- Hepatectomy, Humans, Male, Middle Aged, Tuberculoma diagnosis, Tuberculoma surgery, Tuberculosis, Hepatic diagnosis, Tuberculosis, Hepatic surgery
- Abstract
Background: Liver tuberculosis is a fairly rare manifestation of extra-pulmonary tuberculosis. We distinguish several forms of liver affection by tuberculosis. One of these is liver tuberculoma, the incidence of which is quite rare. The authors present a case of liver tuberculoma as an occupational disease., Case Report: A 63-year-old veterinary doctor, who was diagnosed through a polymerase chain reaction with liver tuberculosis, was treated unsuccessfully with anti-tuberculosis drugs for a period of 8 months. After an earlier relapse of the focus in the liver it grew again and created an abscess (80 x 65 x 80 mm), together with a second satellite focus (20 mm). The patient was therefore indicated for a resection of 3 segments of the right liver lobe. The resection was without complications. The polymerase chain reaction, together with histology, proved the presence of a mycobacterium tuberculosis complex. Three year after the surgery, the patient is completely recovered, without any manifestations of the disease., Conclusion: Liver resection for liver tuberculoma is indicated in case of progression of the finding and long-term unsuccessful treatment with anti-tuberculosis drugs. It is a safe method with very good long-term results (Fig. 2, Ref. 12).
- Published
- 2009
30. Lymphoepithelioma-like carcinoma of the Bartholin gland.
- Author
-
Kacerovska D, Nemcova J, Petrik R, Michal M, and Kazakov DV
- Subjects
- Epithelial Cells pathology, Female, Humans, Middle Aged, Bartholin's Glands pathology, Carcinoma diagnosis, Carcinoma pathology, Lymphocytes pathology, Vulvar Neoplasms diagnosis, Vulvar Neoplasms pathology
- Abstract
The authors report a case of lymphoepithelioma-like carcinoma originating in the Bartholin's gland in a 52-year-old woman. The tumor showed distinct morphologic features including variably sized and shaped nodules and syncytial sheets of large epithelial cells that contained large round nuclei with vesicular chromatin and prominent nucleoli and that were permeated and surrounded by small, well-differentiated lymphocytes and plasma cells. Focally, the neoplastic nodules blended with residual acini of the Bartholin gland. In the transitional areas, there were few acini that evidenced squamous cell metaplasia with partial or complete replacement of mucinous cells by squamous epithelium that showed dysplastic changes. In addition, some ducts in the transitional areas manifested lymphocyte infiltration of their walls. The uninvolved residuum of the Bartholin gland was unremarkable except for a few ruptured ducts or acini with extravasated stromal mucin. Immunohistochemically, the tumor cells were positive for p16, as were acini with metaplastic and dysplastic changes. Neither Epstein-Barr virus nor human papillomavirus was identified in the lesional tissue by polymerase chain reaction. This seems to be the first full-documented report of lymphoepithelioma-like carcinoma originating in the Bartholin gland.
- Published
- 2008
- Full Text
- View/download PDF
31. Mucinous tubular and spindle cell carcinoma with Fuhrman nuclear grade 3: a histological, immunohistochemical, ultrastructural and FISH study.
- Author
-
Kuroda N, Hes O, Michal M, Nemcova J, Gal V, Yamaguchi T, Kawada T, Imamura Y, Hayashi Y, and Lee GH
- Subjects
- Adenocarcinoma, Mucinous genetics, Adenocarcinoma, Mucinous metabolism, Carcinoma, Renal Cell genetics, Carcinoma, Renal Cell metabolism, Fatal Outcome, Female, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Kidney Neoplasms genetics, Kidney Neoplasms metabolism, Microscopy, Electron, Transmission, Middle Aged, Racemases and Epimerases biosynthesis, Adenocarcinoma, Mucinous pathology, Carcinoma, Renal Cell pathology, Kidney Neoplasms pathology
- Abstract
Mucinous tubular and spindle cell carcinoma (MTSCC) of the kidney generally shows low nuclear grade. MTSCC with high nuclear grade is relatively rare. In this article, we report two cases of MTSCC with Fuhrman grade 3. One case occurred in a 57-year-old Japanese female and the second case in a 49-year-old Caucasian female. Histologically, the tumors were composed of neoplastic cells with cuboidal or columnar and spindle morphology, and Fuhrman nuclear grade 3. The myxoid stroma was also observed. This stroma was positive for Alcian blue stain. Immunohistochemically, neoplastic cells of both cases were positive for AMACR, but negative for CD10 and RCC Ma. Ultrastructurally, tumorous cells of one case contained numerous mitochondria. In FISH analysis, many neoplastic cells of both cases demonstrated monosomy of chromosomes 15 and 22 and disomy of chromosomes 7 and 17. One of the two patients died of respiratory failure due to pleuritis carcinomatosa 48 months postoperatively. Finally, the pathologist should recognize that high grade MTSCC exists despite its rare frequency. FISH analysis may be helpful in establishing the diagnosis of this entity. Furthermore, we present the first report of a patient with MTSCC dying of distant metastasis.
- Published
- 2008
- Full Text
- View/download PDF
32. Eccrine syringofibroadenoma associated with well-differentiated squamous cell carcinoma.
- Author
-
Kacerovska D, Nemcova J, Michal M, and Kazakov DV
- Subjects
- Aged, 80 and over, Fibroadenoma diagnosis, Fibroadenoma pathology, Hand, Humans, Male, Neoplasms, Squamous Cell diagnosis, Neoplasms, Squamous Cell pathology, Skin Neoplasms diagnosis, Skin Neoplasms pathology, Sweat Gland Neoplasms diagnosis, Sweat Gland Neoplasms pathology, Syringoma diagnosis, Syringoma pathology, Fibroadenoma complications, Neoplasms, Squamous Cell complications, Skin Neoplasms complications, Sweat Gland Neoplasms complications, Syringoma complications
- Abstract
We report a case of an eccrine syringofibroadenoma (ESFA) associated with well-differentiated squamous cell carcinoma. The patient was an 85-year-old man, who had a 2.5x2.5-cm, brown-colored ulcerated nodule, with a fragile, flesh-colored bleeding surface located beyond the metacarpophalangeal joint of the second finger of his left hand. Histopathologically, there were areas of a well-differentiated squamous cell carcinoma, alternating with the typical area of ESFA characterized by anastomosing cords, strands, and columns of epithelial cells extending from the crusted epidermis into a thickened, edematous, myxoid vascular-rich dermis. Immunohistochemically, the areas with dysplastic epithelium were positive for p16, whereas the benign ESFA parts tested negative. Human papillomavirus was detected in the lesional tissue by polymerase chain reaction, and the subsequent sequencing analysis demonstrated that the virus was close to human papillomavirus type 107.
- Published
- 2008
- Full Text
- View/download PDF
33. Cutaneous and superficial soft tissue lesions associated with Albright hereditary osteodystrophy: clinicopathological and molecular genetic study of 4 cases, including a novel mutation of the GNAS gene.
- Author
-
Kacerovska D, Nemcova J, Pomahacova R, Michal M, and Kazakov DV
- Subjects
- Adult, Bone Neoplasms diagnosis, Bone Neoplasms genetics, Bone Neoplasms pathology, Calcinosis diagnosis, Calcinosis genetics, Calcinosis pathology, Child, Child, Preschool, Chromogranins, Female, Fibroma diagnosis, Fibroma genetics, Fibroma pathology, Fibrous Dysplasia, Polyostotic diagnosis, Humans, Male, Nevus diagnosis, Nevus genetics, Nevus pathology, Osteoma diagnosis, Osteoma genetics, Osteoma pathology, Skin Neoplasms diagnosis, Skin Neoplasms genetics, Skin Neoplasms pathology, Fibrous Dysplasia, Polyostotic genetics, Fibrous Dysplasia, Polyostotic pathology, GTP-Binding Protein alpha Subunits, Gs genetics, Mutation genetics
- Abstract
Albright hereditary osteodystrophy is a rare syndrome, in which cutaneous and superficial soft tissue lesions traditionally include osteomas and calcifications. We report 4 patients from 2 families affected with Albright hereditary osteodystrophy and demonstrate that the spectrum of these cutaneous and soft tissue lesions is broader than is usually defined in the literature. In addition to osteomas in the dermis and subcutis, including so-called plaque-like osteoma, we identified the following lesions: calcifying aponeurotic fibroma-like lesion, calcinosis circumscripta-like lesion, and unusual nevi with osteoid and/or peculiar intranuclear pseudoinclusions. One osteoma and the calcifying aponeurotic fibroma-like lesion were analyzed by HUMARA and proved to be clonal. In a family, a novel mutation in the GNAS gene was also identified.
- Published
- 2008
- Full Text
- View/download PDF
34. Spiradenocylindroma-like basaloid carcinoma of the anus and rectum: case report, including HPV studies and analysis of the CYLD gene mutations.
- Author
-
Kacerovska D, Szepe P, Vanecek T, Nemcova J, Michal M, Mukensnabl P, and Kazakov DV
- Subjects
- Anus Neoplasms diagnosis, Anus Neoplasms virology, Carcinoma, Adenoid Cystic diagnosis, Carcinoma, Adenoid Cystic virology, Carcinoma, Basal Cell diagnosis, Carcinoma, Basal Cell virology, Deubiquitinating Enzyme CYLD, Diagnosis, Differential, Female, Gene Expression Regulation, Neoplastic, Humans, Middle Aged, Mutation genetics, Rectal Neoplasms diagnosis, Rectal Neoplasms virology, Skin Neoplasms diagnosis, Skin Neoplasms virology, Tumor Suppressor Proteins genetics, Anus Neoplasms pathology, Carcinoma, Adenoid Cystic pathology, Carcinoma, Basal Cell pathology, Human papillomavirus 16 pathogenicity, Rectal Neoplasms pathology, Skin Neoplasms pathology, Tumor Suppressor Proteins metabolism
- Abstract
The authors report a case of basaloid carcinoma involving the anus and rectum of a 57-year-old woman. Microscopically, the tumor showed unusual morphologic features strongly resembling a spiradenocylindroma because it consisted, in most parts, of basaloid cell nodules arranged in a jigsaw-puzzle fashion containing or surrounded by eosinophilic basal membrane material; in addition, there were intratumoral lymphocytes. The overlying squamous epithelium manifested dysplastic changes compatible with in situ squamous carcinoma that gradually became invasive and blended with basaloid cell islands; additionally, there were koilocytes in the squamous epithelium. A molecular biology study identified HPV-16 in the lesional tissue. Analysis of the CYLD gene did not prove any mutation.
- Published
- 2008
- Full Text
- View/download PDF
35. Atypical histopathological features in cutaneous lymphoid hyperplasia of the scrotum.
- Author
-
Belousova IE, Nemcova J, Kacerovska D, Michal M, and Kazakov DV
- Subjects
- Adolescent, Adult, Aged, Child, Diagnosis, Differential, Humans, Lymphoma pathology, Male, Middle Aged, Pseudolymphoma pathology, Scrotum pathology, Skin Diseases pathology
- Published
- 2008
- Full Text
- View/download PDF
36. Hidradenoma papilliferum with a ductal carcinoma in situ component: case report and review of the literature.
- Author
-
Vazmitel M, Spagnolo DV, Nemcova J, Michal M, and Kazakov DV
- Subjects
- Adenoma, Sweat Gland metabolism, Adenoma, Sweat Gland virology, Adult, Carcinoma in Situ metabolism, Carcinoma in Situ virology, Carcinoma, Ductal metabolism, Carcinoma, Ductal virology, Female, Human papillomavirus 16, Humans, Immunohistochemistry, Papillomavirus Infections complications, Papillomavirus Infections metabolism, Papillomavirus Infections pathology, Polymerase Chain Reaction, Sweat Gland Neoplasms metabolism, Sweat Gland Neoplasms virology, Vulvar Neoplasms metabolism, Vulvar Neoplasms virology, Adenoma, Sweat Gland pathology, Carcinoma in Situ pathology, Carcinoma, Ductal pathology, Sweat Gland Neoplasms pathology, Vulvar Neoplasms pathology
- Abstract
Hidradenoma papilliferum (HP) is a benign cutaneous adnexal neoplasm occurring mainly in the anogenital region of adult women and has features analogous to intraductal papilloma of the breast. Malignant change in HP is extremely rare. Only a single case of ductal carcinoma in situ arising in HP has been previously reported. We present a new case of HP which, in addition to the typical appearance of HP, contained a focus of ductal carcinoma in situ that appeared as enlarged pleomorphic epithelial cells having a "blastic" appearance, exhibiting atypical mitotic figures and surrounded by myoepithelial cells. Molecular biological study identified human papillomavirus (HPV)-16, which, it may be argued, may have played a role in the development of the carcinoma.
- Published
- 2008
- Full Text
- View/download PDF
37. Human papillomavirus in lesions of anogenital mammary-like glands.
- Author
-
Kazakov DV, Nemcova J, Mikyskova I, Belousova IE, Vazmitel M, and Michal M
- Subjects
- Adult, Aged, Female, Humans, Male, Middle Aged, Papillomaviridae isolation & purification, Polymerase Chain Reaction, Anus Neoplasms virology, Exocrine Glands pathology, Genital Neoplasms, Female virology, Papillomavirus Infections epidemiology, Tumor Virus Infections epidemiology
- Abstract
Long considered as ectopic breast tissue, anogenital mammary-like glands (MLGs) have recently been suggested to represent distinctive structures located in the anogenital area. We studied 16 neoplasms of anogenital MLG for human papillomavirus (HPV) DNA using INNO-line probe assay (LiPA) HPV Genotyping kit, GP5+/6+, CP(SGB), and FAP 6085-6319 primer sets. The lesions included 3 fibroadenomas, 2 adenosis tumors, 1 invasive ductal carcinoma, 1 tubulolobular carcinoma, 2 hidradenoma papilliferum with prominent cystic change rendering a cystadenoma appearance and oxyphilic metaplasia, and 7 cases of extramammary Paget disease. All 3 fibroadenomas, both adenosis tumors, both hidradenoma papilliferum, and the tubulolobular carcinoma proved negative for HPV DNA. HPV-31 was detected by LiPA in the case of invasive ductal carcinoma. In 2 of the 7 patients with extramammary Paget disease, there was HPV DNA present in the lesional tissue, typed as HPV-6 (LiPA) and a type which was closely related to HPV-21 and HPV-24 (FAP 6085-6319), whereas the remaining 5 cases tested negative. These results coupled with those obtained from literature review suggest that HPV plays no causative role in lesions of anogenital MLG.
- Published
- 2007
- Full Text
- View/download PDF
38. Absence of Epstein-Barr virus, human papillomavirus, and simian virus 40 in patients of central european origin with lymphoepithelioma-like carcinoma of the skin.
- Author
-
Kazakov DV, Nemcova J, Mikyskova I, and Michal M
- Subjects
- Aged, Aged, 80 and over, Carcinoma, Squamous Cell secondary, Cell Nucleolus ultrastructure, Czech Republic, Ear Neoplasms virology, Ear, External virology, Epithelial Cells pathology, Facial Neoplasms virology, Female, Follow-Up Studies, Humans, Lymphatic Metastasis pathology, Lymphocytes pathology, Male, Middle Aged, Penile Neoplasms virology, Plasma Cells pathology, Scalp virology, Slovakia, Survival Rate, Alphapapillomavirus isolation & purification, Carcinoma, Squamous Cell virology, Herpesvirus 4, Human isolation & purification, Simian virus 40 isolation & purification, Skin Neoplasms virology
- Abstract
The authors report 10 cases of lymphoepithelioma-like carcinoma (LELC) of the skin and the results of a molecular biological study for HPV, EBV, and SV40 in lesional tissues. All patients originated from Central Europe. There were seven men and three women, ranging in age from 57 to 86 years. Locations included the face (n = 4), scalp (n = 2), penis (n = 2), and retroauricular area (n = 1); location was unknown for one subject. All but two patients presented with a tumor confined to the skin; in both patients with the penile carcinoma, the tumors had metastasized to an inguinal lymph node. Six patients with available follow-up included four individuals with no evidence of tumor metastasis or recurrences at 2, 3, 4, and 5 years, one patient who died with metastatic disease 7 years after diagnosis, and one patient who died of an unrelated course. Microscopically, all cases showed distinctive features of LELC characterized by variably sized and shaped nodules or syncytial sheets of epithelial cells that contained vesicular chromatic and prominent nucleoli and that were permeated and surrounded by small, well-differentiated lymphocytes and plasma cells. Because all 10 cases studied proved negative for EBV, HPV, and SV40, these viruses seem to play no causal role in LELC of the skin in patients from Central Europe.
- Published
- 2007
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.