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2,204 results on '"Neri, Giovanni"'

2. Autologous anti-GD2 CAR T cells efficiently target primary human glioblastoma

Author

Chiavelli, Chiara, Prapa, Malvina, Rovesti, Giulia, Silingardi, Marco, Neri, Giovanni, Pugliese, Giuseppe, Trudu, Lucia, Dall’Ora, Massimiliano, Golinelli, Giulia, Grisendi, Giulia, Vinet, Jonathan, Bestagno, Marco, Spano, Carlotta, Papapietro, Roberto Vito, Depenni, Roberta, Di Emidio, Katia, Pasetto, Anna, Nascimento Silva, Daniela, Feletti, Alberto, Berlucchi, Silvia, Iaccarino, Corrado, Pavesi, Giacomo, and Dominici, Massimo

Published
2024
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19. Incidental findings on brain MRI in patients with first-episode and chronic psychosis

Author

Bellani, Marcella, Perlini, Cinzia, Zovetti, Niccolò, Rossetti, Maria Gloria, Alessandrini, Franco, Barillari, Marco, Ricciardi, Giuseppe Kenneth, Konze, Angela, Sberna, Maurizio, Zoccatelli, Giada, Lasalvia, Antonio, Miceli, Maurizio, Neri, Giovanni, Torresani, Stefano, Mazzi, Fausto, Scocco, Paolo, D'Agostino, Armando, Imbesi, Massimiliano, Veronese, Angela, Ruggeri, Mirella, and Brambilla, Paolo

Published
2022
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25. A Phenylalanine Sensor Exploiting a Capacitive Readout Strategy Embedding a Selective Enzymatic Mechanism

Author

Petralia, Salvatore, Castorina, Salvatore, Maugeri, Ludovica, Messina, Maria Anna, Ruggieri, Martino, Neri, Giovanni, Ferlazzo, Angelo, Sardini, Emilio, Serpelloni, Mauro, Bellitti, Paolo, and Ando, Bruno

Abstract

This article introduces a cost-effective and fast-response capacitive sensor designed for quantifying phenylalanine (Phe) levels in PKU patients. The sensor comprises planar interdigitated electrodes (IDEs) on a substrate, with NH3-sensitive dielectric material, and exploits an enzymatic reaction to indirectly assess Phe levels. IDEs are created using aerosol jet printing (AJP), with two variants differing in the type of ink used. Experimental results achieved by exposing sensors to standard NH3 and Phe solutions have been reported. The proposed approach provides a capacitive readout strategy for low analyte concentrations, high specificity to NH3 using yttria-stabilized zirconia (YSZ), easy miniaturization, faster response times, and cost-effectiveness through rapid-prototyping technologies. A sensor limit of detection of $0.52~\mu $ M has been estimated for Phe detection, along with an accuracy of $6.27~\mu $ M.

Published
2024
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27. Mitochondrial Dysfunction Causes Cell Death in Patients Affected by Fragile-X-Associated Disorders

Author

Grandi, Martina, Galber, Chiara, Gatto, Cristina, Nobile, Veronica, Pucci, Cecilia, Schaldemose Nielsen, Ida, Boldrin, Francesco, Neri, Giovanni, Chiurazzi, Pietro, Solaini, Giancarlo, Baracca, Alessandra, Giorgio, Valentina, Tabolacci, Elisabetta, Chiurazzi, Pietro (ORCID:0000-0001-5104-1521), Tabolacci, Elisabetta (ORCID:0000-0002-4707-2242), Grandi, Martina, Galber, Chiara, Gatto, Cristina, Nobile, Veronica, Pucci, Cecilia, Schaldemose Nielsen, Ida, Boldrin, Francesco, Neri, Giovanni, Chiurazzi, Pietro, Solaini, Giancarlo, Baracca, Alessandra, Giorgio, Valentina, Tabolacci, Elisabetta, Chiurazzi, Pietro (ORCID:0000-0001-5104-1521), and Tabolacci, Elisabetta (ORCID:0000-0002-4707-2242)

Abstract

Mitochondria are involved in multiple aspects of neurodevelopmental processes and play a major role in the pathogenetic mechanisms leading to neuro-degenerative diseases. Fragile-X-related disorders (FXDs) are genetic conditions that occur due to the dynamic expansion of CGG repeats of the FMR1 gene encoding for the RNA-binding protein FMRP, particularly expressed in the brain. This gene expansion can lead to premutation (PM, 56-200 CGGs), full mutation (FM, >200 CGGs), or unmethylated FM (UFM), resulting in neurodegeneration, neurodevelopmental disorders, or no apparent intellectual disability, respectively. To investigate the mitochondrial mechanisms that are involved in the FXD patients, we analyzed mitochondrial morphology and bioenergetics in fibroblasts derived from patients. Donut-shaped mitochondrial morphology and excessive synthesis of critical mitochondrial proteins were detected in FM, PM, and UFM cells. Analysis of mitochondrial oxidative phosphorylation in situ reveals lower respiration in PM fibroblasts. Importantly, mitochondrial permeability transition-dependent apoptosis is sensitized to reactive oxygen species in FM, PM, and UFM models. This study elucidated the mitochondrial mechanisms that are involved in the FXD phenotypes, and indicated altered mitochondrial function and morphology. Importantly, a sensitization to permeability transition and apoptosis was revealed in FXD cells. Overall, our data suggest that mitochondria are novel drug targets to relieve the FXD symptoms.

Published
2024

28. Novel Bioplastic Based on PVA Functionalized with Anthocyanins: Synthesis, Biochemical Properties and Food Applications.

Author

Patanè, Giuseppe Tancredi, Calderaro, Antonella, Putaggio, Stefano, Ginestra, Giovanna, Mandalari, Giuseppina, Cirmi, Santa, Barreca, Davide, Russo, Annamaria, Gervasi, Teresa, Neri, Giovanni, Chelly, Meryam, Visco, Annamaria, Scolaro, Cristina, Mancuso, Francesca, Ficarra, Silvana, Tellone, Ester, and Laganà, Giuseppina

Subjects
FOURIER transform infrared spectroscopy, CHEMICAL processes, CIRCULAR economy, FOOD packaging, RESPONSE surfaces (Statistics), ANTHOCYANINS
Abstract

Over the last ten years, researchers' efforts have aimed to replace the classic linear economy model with the circular economy model, favoring green chemical and industrial processes. From this point of view, biologically active molecules, coming from plants, flowers and biomass, are gaining considerable value. In this study, firstly we focus on the development of a green protocol to obtain the purification of anthocyanins from the flower of Callistemon citrinus, based on simulation and on response surface optimization methodology. After that, we utilize them to manufacture and add new properties to bioplastics belonging to class 3, based on modified polyvinyl alcohol (PVA) with increasing amounts from 0.10 to 1.00%. The new polymers are analyzed to monitor morphological changes, optical properties, mechanical properties and antioxidant and antimicrobial activities. Fourier transform infrared spectroscopy (FTIR) spectra of the new materials show the characteristic bands of the PVA alone and a modification of the band at around 1138 cm−1 and 1083 cm−1, showing an influence of the anthocyanins' addition on the sequence with crystalline and amorphous structures of the starting materials, as also shown by the results of the mechanical tests. These last showed an increase in thickening (from 29.92 μm to approx. 37 μm) and hydrophobicity with the concomitant increase in the added anthocyanins (change in wettability with water from 14° to 31°), decreasing the poor water/moisture resistance of PVA that decreases its strength and limits its application in food packaging, which makes the new materials ideal candidates for biodegradable packaging to extend the shelf-life of food. The functionalization also determines an increase in the opacity, from 2.46 to 3.42 T%/mm, the acquisition of antioxidant activity against 2,2-diphenyl-1-picrylhdrazyl and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radicals and, in the ferric reducing power assay, the antimicrobial (bactericidal) activity against different Staphylococcus aureus strains at the maximum tested concentration (1.00% of anthocyanins). On the whole, functionalization with anthocyanins results in the acquisition of new properties, making it suitable for food packaging purposes, as highlighted by a food fresh-keeping test. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Choriocapillaris in Age-Related Macular Degeneration.

Author

Neri, Giovanni, Olivieri, Chiara, Serafino, Sonia, Viggiano, Pasquale, Marolo, Paola, Reibaldi, Michele, and Borrelli, Enrico

Subjects
*UVEA, *VASCULAR endothelial growth factors, *VISION disorders, *RETINAL degeneration, *OPTICAL coherence tomography, *AGE distribution, *ANGIOGRAPHY, *BLOOD circulation, *PATHOLOGIC neovascularization, *PHOTORECEPTORS, *DISEASE risk factors, *DISEASE complications
Abstract

Age-related macular degeneration (AMD) is a multifactorial disease characterized by progressive alterations of different retinal structures ultimately leading to vision loss. Among these, the choriocapillaris (CC) has been found to be affected in different stages of AMD. In this review we provide a discussion on the different stages of AMD, focusing particularly on the alterations involving the CC. This has been possible thanks to the introduction of optical coherence tomography-angiography, a recently developed imaging technique which allows the detection of blood flow in choroidal vessels. Therefore, the aim of this review is to provide a description of the various alterations involving the CC in the different stages of AMD. [ABSTRACT FROM AUTHOR]

Published
2024
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30. Modified Gold Screen-Printed Electrodes for the Determination of Heavy Metals.

Author

Celesti, Consuelo, Giofrè, Salvatore Vincenzo, Espro, Claudia, Legnani, Laura, Neri, Giovanni, and Iannazzo, Daniela

Subjects
GOLD electrodes, HEAVY metals, METAL detectors, METAL ions, SQUARE waves
Abstract

Screen-printed electrodes (SPEs) are reliable, portable, affordable, and versatile electrochemical platforms for the real-time analytical monitoring of emerging analytes in the environmental, clinical, and agricultural fields. The aim of this study was to evaluate the electrochemical behavior of gold screen-printed electrodes (SPGEs) modified with molecules containing amino (Tr-N) or α-aminophosphonate (Tr-P) groups for the selective and sensitive detection of the toxic metal ions Pb2+ and Hg2+ in aqueous samples. After optimizing the analytical parameters (conditioning potential and time, deposition potential and time, pH and concentration of the supporting electrolyte), anodic square wave stripping voltammetry (SWASV) was used to evaluate and compare the electrochemical performance of bare or modified electrodes for the detection of Hg2+ and Pb2+, either alone or in their mixtures in the concentration range between 1 nM and 10 nM. A significative improvement in the detection ability of Pb2+ ions was recorded for the amino-functionalized gold sensor SPGE-N, while the presence of a phosphonate moiety in SPGE-P led to greater sensitivity towards Hg2+ ions. The developed sensors allow the detection of Pb2+ and Hg2+ with a limit of detection (LOD) of 0.41 nM and 35 pM, respectively, below the legal limits for these heavy metal ions in drinking water or food, while the sensitivity was 5.84 µA nM−1cm−2 and 10 µA nM−1cm−2, respectively, for Pb2+ and Hg2+. The reported results are promising for the development of advanced devices for the in situ and cost-effective monitoring of heavy metals, even in trace amounts, in water resources. [ABSTRACT FROM AUTHOR]

Published
2024
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31. Conductometric H 2 S Sensors Based on TiO 2 Nanoparticles.

Author

Alaya, Yassine, Madani, Malek, Bouguila, Noureddine, El Mir, Lassaad, Fazio, Enza, Corsaro, Carmelo, and Neri, Giovanni

Subjects
TITANIUM dioxide, RAMAN scattering, DETECTORS, FOURIER transform infrared spectroscopy, BAND gaps, CALCINATION (Heat treatment)
Abstract

High-performance hydrogen sulfide (H2S) sensors are mandatory for many industrial applications. However, the development of H2S sensors still remains a challenge for researchers. In this work, we report the study of a TiO2-based conductometric sensor for H2S monitoring at low concentrations. TiO2 samples were first synthesized using the sol-gel route, annealed at different temperatures (400 and 600 °C), and thoroughly characterized to evaluate their morphological and microstructural properties. Scanning electronic microscopy, Raman scattering, X-ray diffraction, and FTIR spectroscopy have demonstrated the formation of clusters of pure anatase in the TiO2 phase. Increasing the calcination temperature to 600 °C enhanced TiO2 crystallinity and particle size (from 11 nm to 51 nm), accompanied by the transition to the rutile phase and a slight decrease in band gap (3.31 eV for 400 °C to 3.26 eV for 600 °C). Sensing tests demonstrate that TiO2 annealed at 400 °C displays good performances (sensor response Ra/Rg of ~3.3 at 2.5 ppm and fast response/recovery of 8 and 23 s, respectively) for the detection of H2S at low concentrations in air. [ABSTRACT FROM AUTHOR]

Published
2024
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38. Mitochondrial Dysfunction Causes Cell Death in Patients Affected by Fragile-X-Associated Disorders

Author

Grandi, Martina, primary, Galber, Chiara, additional, Gatto, Cristina, additional, Nobile, Veronica, additional, Pucci, Cecilia, additional, Schaldemose Nielsen, Ida, additional, Boldrin, Francesco, additional, Neri, Giovanni, additional, Chiurazzi, Pietro, additional, Solaini, Giancarlo, additional, Baracca, Alessandra, additional, Giorgio, Valentina, additional, and Tabolacci, Elisabetta, additional

Published
2024
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47. Altered mitochondrial function in cells carrying a premutation or unmethylated full mutation of the FMR1 gene

Author

Nobile, Veronica, Palumbo, Federica, Lanni, Stella, Ghisio, Valentina, Vitali, Alberto, Castagnola, Massimo, Marzano, Valeria, Maulucci, Giuseppe, De Angelis, Claudio, De Spirito, Marco, Pacini, Laura, D’Andrea, Laura, Ragno, Rino, Stazi, Giulia, Valente, Sergio, Mai, Antonello, Chiurazzi, Pietro, Genuardi, Maurizio, Neri, Giovanni, and Tabolacci, Elisabetta

Published
2020
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48. GD2 CAR T cells against human glioblastoma

Author

Prapa, Malvina, Chiavelli, Chiara, Golinelli, Giulia, Grisendi, Giulia, Bestagno, Marco, Di Tinco, Rosanna, Dall’Ora, Massimiliano, Neri, Giovanni, Candini, Olivia, Spano, Carlotta, Petrachi, Tiziana, Bertoni, Laura, Carnevale, Gianluca, Pugliese, Giuseppe, Depenni, Roberta, Feletti, Alberto, Iaccarino, Corrado, Pavesi, Giacomo, and Dominici, Massimo

Published
2021
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