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2 results on '"Neto YAAH"'

1. Chemosensitizing Effect of Cernumidine Extracted from Solanum cernuum on Bladder Cancer Cells in Vitro.

Author

Miranda MA, Mondal A, Sachdeva M, Cabral H, Neto YAAH, Khan I, Groppo M, McChesney JD, and Bastos JK

Subjects
Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic isolation & purification, Apoptosis drug effects, Caffeic Acids chemistry, Caffeic Acids isolation & purification, Cell Movement drug effects, Cell Proliferation drug effects, Drug Screening Assays, Antitumor, Guanidines chemistry, Guanidines isolation & purification, Humans, Membrane Potential, Mitochondrial drug effects, Microscopy, Fluorescence, Mitochondria drug effects, Mitochondria metabolism, Plant Leaves chemistry, Tumor Cells, Cultured, Urinary Bladder Neoplasms metabolism, Urinary Bladder Neoplasms pathology, Antineoplastic Agents, Phytogenic pharmacology, Caffeic Acids pharmacology, Guanidines pharmacology, Solanum chemistry, Urinary Bladder Neoplasms drug therapy
Abstract

Cernumidine (CER) is a guanidinic alkaloid isolated from Solanum cernuum leaves. In this work, we investigated the cytotoxicity, chemosensitizing effect of cernumidine to cisplatin (cDDP) and the possible mechanism of action of the combination on bladder cancer cells. Cernumidine showed cytotoxicity and could sensitize bladder cancer cells to cisplatin. The combination of CER+cDDP inhibited cell migration on T24 cells. CER+cDDP down-regulated MMP-2/9 and p-ERK1/2, while it increased EGFR activity corroborating the observed cell migration inhibition. Down-regulation of Bcl-2 and up-regulation pro-apoptotic Bax and further depletion of the mitochondrial membrane potential (ΔΨm) indicates that mitochondria play a central role in the combination treatment inducing the mitochondrial signaling pathway of apoptosis in T24 cells. Our data showed that the alkaloid cernumidine is worthy of further studies as a chemosensitizing agent to be used in complementary chemotherapy., (© 2019 Wiley-VHCA AG, Zurich, Switzerland.)

Published
2019
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2. Peptides with antioxidant properties identified from casein, whey, and egg albumin hydrolysates generated by two novel fungal proteases.

Author

Neto YAAH, Rosa JC, and Cabral H

Subjects
Albumins pharmacology, Animals, Antioxidants pharmacology, Caseins pharmacology, Egg Proteins pharmacology, Eupenicillium enzymology, Peptide Hydrolases chemistry, Peptides pharmacology, Proteolysis, Sordariales enzymology, Whey Proteins pharmacology, Albumins chemistry, Antioxidants chemistry, Caseins chemistry, Egg Proteins chemistry, Peptides chemistry, Whey Proteins chemistry
Abstract

There are many diseases linked to oxidative stress, including cancer. Importantly, endogenous antioxidants are insufficient to protect against this process. Peptides derived from food proteins produced by hydrolysis have been investigated as exogenous antioxidants. The present study aimed to identify novel peptides with antioxidant potential produced from egg and milk proteins hydrolysis with two new fungal proteases isolated from Eupenicillium javanicum and Myceliophthora thermophila . The degree of hydrolysis at several time points was calculated and correlated to DPPH scavenging and metal chelating assays, all hydrolysates presented antioxidant activity. Casein hydrolyzed by the M. thermophila protease showed the best antioxidant activity. The identified sequences showed that the proportions of amino acids that influence antioxidant activity support the antioxidant assay. Our data reveal the conditions necessary for the successful generation of antioxidant peptides using two novel fungal proteases. This opens a potential new avenue for the design and manufacture of antioxidant molecules.

Published
2019
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