1. Relationship of Inflammatory Mediators (Interleukin and Cortisol Concentrations) with Corneal Epithelial Quantifiable Metrics
- Author
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Marcony R. Santhiago, MD, PhD, Larissa R. Stival, MD, PhD, Daniella C. Araujo, PhD, Rosalia Antunes-Foschini, MD, PhD, Marcia C. Toledo, MD, Ianne L.S. Nunes, MS, Claudia R. Morgado, MD, and Newton Kara-Junior, MD, PhD
- Subjects
Epithelial map ,Cortisol, Interleukin 6 ,Keratoconus ,Ophthalmology ,RE1-994 - Abstract
Purpose: To investigate the relationship of inflammatory biomarkers with corneal epithelial quantifiable metrics in patients with keratoconus and in healthy eyes. Design: Prospective observational comparative study. Participants: This study included 100 eyes of 100 patients: 48 eyes of 48 patients with keratoconus and 52 healthy eyes of 52 healthy controls. Methods: The concentrations of tear cytokines were investigated in both groups: interleukin (IL) 1B, IL6, IL8, IL10, IL12p70, and tumor necrosis factor α (TNFα) were obtained by capillary flow and measured using flow cytometer. Cortisol concentrations were determined in both groups from the most proximal hair segment as an index of cumulative secretion and measured by liquid chromatography mass spectrometry. Epithelial variables were obtained with OCT. Pearson correlation (r) was used to measure linear dependence between 2 different variables. Main Outcome Measures: Investigating the existence, strength, and significance of any correlation between inflammatory biomarkers (IL1B, IL6, IL8, IL10, IL12p70, TNFα, and hair cortisol concentration) and OCT corneal epithelial quantifiable variables such as minimum and maximum epithelial thickness of the map, difference between the minimum and maximum (Epithelial Min-Max) and standard deviation of the epithelial thickness of the map (Epithelial Std Dev), and average epithelial thickness of the superior and inferior regions of the map. Results: Eyes with keratoconus presented statistically significantly higher levels of IL1b (P = 0.02), IL6 (P < 0.0001), IL8 (P < 0.0001), and TNFα (P < 0.0001) and hair cortisol concentration (P = 0.01) compared with healthy controls.There was a significant correlation between IL6 and measurement Epithelium Min-Max [Pearson = −0.59 (−0.69, −0.47); P
- Published
- 2025
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