Search

Your search keyword '"Ng, Ching Ging"' showing total 12 results
Start Over Author "Ng, Ching Ging"
12 results on '"Ng, Ching Ging"'

3. The Cellular Characterization of SARS-CoV-2 Spike Protein in Virus-Infected Cells Using the Receptor Binding Domain Binding Specific Human Monoclonal Antibodies

Author

Chan, Conrad En-Zuo, primary, Ng, Ching-Ging, additional, Lim, Angeline Pei-Chew, additional, Seah, Shirley Lay-Kheng, additional, Chye, De-Hoe, additional, Wong, Steven Ka-Khuen, additional, Lim, Jie-Hui, additional, Lim, Vanessa Zi-Yun, additional, Lai, Soak-Kuan, additional, Wong, Pui-San, additional, Leong, Kok-Mun, additional, Liu, Yi-Chun, additional, Sugrue, Richard J., additional, and Tan, Boon-Huan, additional

Published
2022
Full Text
View/download PDF

4. The cellular characterisation of SARS-CoV-2 spike protein in virus-infected cells using Receptor Binding Domain-binding specific human monoclonal antibodies.

Author

Chan, Conrad En-Zuo, primary, Ng, Ching-Ging, additional, Lim, Angeline P. C., additional, Seah, Shirley Gek-Kheng, additional, Chye, De Hoe, additional, Wong, Steven K. K., additional, Lim, Jie-Hui, additional, Lim, Vanessa Zi-Yun, additional, Lai, Soak Kuan, additional, Wong, Pui-San, additional, Leong, Kok-Mun, additional, Liu, Yichun, additional, Sugrue, Richard, additional, and Tan, Boon Huan, additional

Published
2021
Full Text
View/download PDF

6. Lack of viable severe acute respiratory coronavirus virus 2 (SARS-CoV-2) among PCR-positive air samples from hospital rooms and community isolation facilities

Author

Ong, Sean Wei Xiang, primary, Tan, Yian Kim, additional, Coleman, Kristen Kelli, additional, Tan, Boon Huan, additional, Leo, Yee-Sin, additional, Wang, Dong Ling, additional, Ng, Ching Ging, additional, Ng, Oon-Tek, additional, Wong, Michelle Su Yen, additional, and Marimuthu, Kalisvar, additional

Published
2021
Full Text
View/download PDF

7. Environmental contamination in a coronavirus disease 2019 (COVID-19) intensive care unit—What is the risk?

Author

Ong, Sean Wei Xiang, primary, Lee, Pei Hua, additional, Tan, Yian Kim, additional, Ling, Li Min, additional, Ho, Benjamin Choon Heng, additional, Ng, Ching Ging, additional, Wang, Dong Ling, additional, Tan, Boon Huan, additional, Leo, Yee-Sin, additional, Ng, Oon-Tek, additional, Wong, Michelle Su Yen, additional, and Marimuthu, Kalisvar, additional

Published
2020
Full Text
View/download PDF

8. Environmental contamination in a coronavirus disease 2019 (COVID-19) intensive care unit—What is the risk?

Author

Ong, Sean Wei Xiang, Lee, Pei Hua, Tan, Yian Kim, Ling, Li Min, Ho, Benjamin Choon Heng, Ng, Ching Ging, Wang, Dong Ling, Tan, Boon Huan, Leo, Yee-Sin, Ng, Oon-Tek, Wong, Michelle Su Yen, and Marimuthu, Kalisvar

Abstract

AbstractBackground:The risk of environmental contamination by severe acute respiratory coronavirus virus 2 (SARS-CoV-2) in the intensive care unit (ICU) is unclear. We evaluated the extent of environmental contamination in the ICU and correlated this with patient and disease factors, including the impact of different ventilatory modalities.Methods:In this observational study, surface environmental samples collected from ICU patient rooms and common areas were tested for SARS-CoV-2 by polymerase chain reaction (PCR). Select samples from the common area were tested by cell culture. Clinical data were collected and correlated to the presence of environmental contamination. Results were compared to historical data from a previous study in general wards.Results:In total, 200 samples from 20 patient rooms and 75 samples from common areas and the staff pantry were tested. The results showed that 14 rooms had at least 1 site contaminated, with an overall contamination rate of 14% (28 of 200 samples). Environmental contamination was not associated with day of illness, ventilatory mode, aerosol-generating procedures, or viral load. The frequency of environmental contamination was lower in the ICU than in general ward rooms. Eight samples from the common area were positive, though all were negative on cell culture.Conclusion:Environmental contamination in the ICU was lower than in the general wards. The use of mechanical ventilation or high-flow nasal oxygen was not associated with greater surface contamination, supporting their use and safety from an infection control perspective. Transmission risk via environmental surfaces in the ICUs is likely to be low. Nonetheless, infection control practices should be strictly reinforced, and transmission risk via droplet or airborne spread remains.

Published
2021
Full Text
View/download PDF

10. Outbreak of caliciviruses in the Singapore military, 2015.

Author

Jun Xian Neo, Freddy, Jin Phang Loh, Jimmy, Peijun Ting, Wei Xin Yeo, Qiu Han Gao, Christine, Jian Ming Lee, Vernon, Boon Huan Tan, Ching Ging Ng, Neo, Freddy Jun Xian, Loh, Jimmy Jin Phang, Ting, Peijun, Yeo, Wei Xin, Gao, Christine Qiu Han, Lee, Vernon Jian Ming, Tan, Boon Huan, and Ng, Ching Ging

Subjects
CALICIVIRUSES, DISEASE outbreaks, HEALTH of military personnel, INFECTIOUS disease transmission, BIOLOGICAL evolution, FOOD handling, GASTROENTERITIS, HAND washing, RNA viruses, MILITARY personnel, STERILIZATION (Disinfection), GENOTYPES, NOROVIRUS diseases, PREVENTION
Abstract

Background: From 31 August to 9 September 2015, a total of 150 military personnel at a military institution in Singapore were infected with acute gastroenteritis (AGE) with an attack rate of approximately 3%. This study aimed to determine the epidemiology of the outbreak, investigate its origins, and discuss measures to prevent future occurrences.Methods: After the AGE outbreak was declared on 31 August 2015, symptom surveys, hygiene inspections, and the testing of water, food, and stool samples were initiated. We collected 86 stool samples from AGE cases and 58 samples from food-handlers during the course of the outbreak and these stool samples were tested for 8 bacterial pathogens and 2 viral pathogens (i.e., norovirus and sapovirus).Results: We detected Sapovirus (SaV), group I Norovirus (NoV GI) and group II Norovirus (NoV GII) from the stool samples of AGE cases. Further sequence analyses showed that the AGE outbreak in August was caused mainly by three rarely reported calicivirus novel genotypes: NoV GI.7, NoV GII.17 and SaV GII.3. Control measures implemented focused on the escalation of personal and environmental hygiene, which included the separation of affected and unaffected soldiers, enforcement of rigorous hand-washing and hygiene, raising awareness of food and water safety, and disinfection of communal areas with bleach.Conclusions: This study identified both NoV and SaV as the causative agents for an AGE outbreak at a Singapore military camp in August 2015. This study is also the first to report SaV as one of the main causative agents, highlighting the importance of caliciviruses as causative agents of AGE outbreaks in the Singapore military. As there are no commercially available vaccines against caliciviruses, strict personal hygiene and proper disinfection of environmental surfaces remain crucial to prevent calicivirus outbreak and transmission. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

12. Antibiotic susceptibility of mammalian mitochondrial translation

Author

Taeko Komoda, Li Zhang, Takao Hanada, Tsutomu Suzuki, Ng Ching Ging, and Kimitsuna Watanabe

Subjects
medicine.drug_class, Mitochondrial translation, Antibiotics, Biophysics, Mitochondrion, Biology, Biochemistry, Ribosome, Thiostrepton, Microbiology, chemistry.chemical_compound, Structural Biology, Drug Resistance, Bacterial, Genetics, medicine, Mitochondrial ribosome, Protein biosynthesis, Escherichia coli, Animals, Molecular Biology, E. coli, Translation (biology), Cell Biology, Peptide Elongation Factors, Anti-Bacterial Agents, Mitochondria, chemistry, Protein Biosynthesis, Cattle, Protein synthesis, Ribosomes
Abstract

All medically useful antibiotics should have the potential to distinguish between target microbes (bacteria) and host cells. Although many antibiotics that target bacterial protein synthesis show little effect on the translation machinery of the eukaryotic cytoplasm, it is unclear whether these antibiotics target or not the mitochondrial translation machinery. We employed an in vitro translation system from bovine mitochondria, which consists of mitochondrial ribosomes and mitochondrial elongation factors, to estimate the effect of antibiotics on mitichondrial protein synthesis. Tetracycline and thiostrepton showed similar inhibitory effects on both Escherichia coli and mitochondrial protein synthesis. The mitochondrial system was more resistant to tiamulin, macrolides, virginiamycin, fusidic acid and kirromycin than the E. coli system. The present results, taken together with atomic structure of the ribosome, may provide useful information for the rational design of new antibiotics having less adverse effects in humans and animals.

Published
2005

Catalog

Books, media, physical & digital resources
See catalog results