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Your search keyword '"Nguyen, Linda T."' showing total 27 results
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27 results on '"Nguyen, Linda T."'

2. Combined TP53 mutation/3p loss correlates with decreased radiosensitivity and increased matrix-metalloproteinase activity in head and neck carcinoma

Author

Raju, Sharat C, Hauff, Samantha J, Lemieux, Aaron J, Orosco, Ryan K, Gross, Andrew M, Nguyen, Linda T, Savariar, Elamprakash, Moss, William, Whitney, Michael, Cohen, Ezra E, Lippman, Scott M, Tsien, Roger Y, Ideker, Trey, Advani, Sunil J, and Nguyen, Quyen T

Subjects
Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Cancer, Rare Diseases, Genetics, Dental/Oral and Craniofacial Disease, Acid Anhydride Hydrolases, Carcinoma, Squamous Cell, Cell Line, Tumor, Chromosome Deletion, Chromosomes, Human, Pair 3, Genes, p53, Head and Neck Neoplasms, Humans, Matrix Metalloproteinases, Mutation, Neoplasm Proteins, Radiation Tolerance, Head and neck cancer, TP53 mutation, 3p Deletion, FHIT, Matrix metalloproteinases, "double-hit'', "single-hit'', Double-stranded DNA breaks, RNA interference, Radiosensitivity, “double-hit”, “single-hit”, Dentistry, Public Health and Health Services, Oncology & Carcinogenesis, Oncology and carcinogenesis
Abstract

ObjectivePatients with head and neck squamous cell carcinoma (HNSCC) containing TP53 mutation and 3p deletion ("double-hit") have poorer prognosis compared to patients with either event alone ("single-hit"). The etiology for worse clinical outcomes in patients with "double-hit" cancers is unclear. We compared radiosensitivity of cell lines containing both TP53 mutations and deletion of Fragile Histidine Triad (FHIT, the gene most commonly associated with 3p deletion) to "single-hit" lines with only TP53 mutation. We compared radiosensitivity in a "single-hit" cell line with TP53 mutation converted to "double-hit" using RNA interference targeting FHIT. Finally, we compared matrixmetalloproteinase-2/9 (MMP-2/9) activity, a previously-established biomarker for tumor aggressiveness, in xenograft tumors derived from these cell lines.Materials/methodsTP53 mutation and FHIT deletion profiles of HNSCC lines were established using Cancer Cell Line Encyclopedia (CCLE). We used RNA-interference to convert a "single-hit" cell line (SCC4) to "double-hit". Cultured cells were examined for radiosensitivity and cisplatin sensitivity. MMP-2/9 activity was evaluated in "double-hit" versus "single-hit" tumors using ratiometric activatable cell-penetrating peptide (RACPP) in tongue (n=17) and flank xenografts (n=4).ResultsRadiotherapy caused greater double-stranded DNA breaks in "single-hit" vs naturally occurring and engineered "double-hit" cells. In-vivo, "double-hit" xenografts demonstrated higher MMP-2/9 activity compared to "single-hit" xenografts (p

Published
2015

5. Lung transcriptional unresponsiveness and loss of early influenza virus control in infected neonates is prevented by intranasal Lactobacillus rhamnosus GG

Author

Kumova, Ogan K., primary, Fike, Adam J., additional, Thayer, Jillian L., additional, Nguyen, Linda T., additional, Mell, Joshua Chang, additional, Pascasio, Judy, additional, Stairiker, Christopher, additional, Leon, Leticia G., additional, Katsikis, Peter D., additional, and Carey, Alison J., additional

Published
2019
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15. Tu2092 Contrasting Gastroparesis in Type 1 (T1DM) vs. Type 2 (T2DM) Diabetes: Clinical Course After 48 Weeks of Follow-up and Relation to Comorbidities and Health Resource Utilization

Author

Koch, Kenneth L., primary, Hasler, William L., additional, Yates, Katherine P., additional, Calles-Escandon, Jorge, additional, Parkman, Henry P., additional, Abell, Thomas L., additional, Pasricha, Pankaj J., additional, McCallum, Richard W, additional, Snape, William J., additional, Nguyen, Linda T., additional, Sarosiek, Irene, additional, Farrugia, Gianrico, additional, Lee, Linda A., additional, Tonascia, James, additional, Unalp, Aynur, additional, Hamilton, Frank A., additional, and Consortium, GpCRC, additional

Published
2013
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16. Mo2093 Complementary and Alternative Medicine Use in Patients Enrolled in Gastroparesis Registry

Author

Lee, Linda A., primary, Unalp, Aynur, additional, Corless, Erin, additional, Yates, Katherine P., additional, Parkman, Henry P., additional, Abell, Thomas L., additional, Koch, Kenneth L., additional, Hasler, William L., additional, Pasricha, Pankaj J., additional, Snape, William J., additional, Nguyen, Linda T., additional, McCallum, Richard W., additional, Sarosiek, Irene, additional, Farrugia, Gianrico, additional, Tonascia, James, additional, Hamilton, Frank A., additional, and Consortium, GpCRC, additional

Published
2013
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19. Characterization of CD31 expression on murine and human neonatal T lymphocytes during development and activation

Author

Fike, Adam J, Nguyen, Linda T, Kumova, Ogan K, and Carey, Alison J

Abstract

BackgroundCD31, expressed by the majority of the neonatal T-cell pool, is involved in modulation of T-cell receptor signaling by increasing the threshold for T-cell activation. Therefore, CD31 could modulate neonatal tolerance and adaptive immune responses.MethodsLymphocytes were harvested from murine neonates at different ages, human late preterm and term cord blood, and adult peripheral blood. Human samples were activated over a 5-day period to simulate acute inflammation. Mice were infected with influenza; lungs and spleens were harvested at days 6 and 9 post infection and analyzed by flow cytometry.ResultsCD31-expressing neonatal murine CD4+and CD8a+T cells increase over the first week of life. Upon in vitro stimulation, human infants’ CD4+and CD8a+T cells shed CD31 faster in comparison with adults. In the context of acute infection, mice infected at 3 days of age have an increased number of naive and activated CD31+T lymphocytes at the site of infection at days 6 and 9 post infection, as compared with those infected at 7 days of age; however, the opposite is true in the periphery.ConclusionDifferences in trafficking of CD31+cytotoxic T lymphocytes (CTLs) during acute influenza infection could modulate tolerance and contribute to a dampened adaptive immune response in neonates.

Published
2017
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