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2. Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes

Author

Benjamin B. Johnson, Marie-Victoire Cosson, Lorenza I. Tsansizi, Terri L. Holmes, Tegan Gilmore, Katherine Hampton, Ok-Ryul Song, Nguyen T.N. Vo, Aishah Nasir, Alzbeta Chabronova, Chris Denning, Mandy J. Peffers, Catherine L.R. Merry, John Whitelock, Linda Troeberg, Stuart A. Rushworth, Andreia S. Bernardo, and James G.W. Smith

Subjects
CP: Developmental biology, CP: Cell biology, Biology (General), QH301-705.5
Abstract

Summary: Perlecan (HSPG2), a heparan sulfate proteoglycan similar to agrin, is key for extracellular matrix (ECM) maturation and stabilization. Although crucial for cardiac development, its role remains elusive. We show that perlecan expression increases as cardiomyocytes mature in vivo and during human pluripotent stem cell differentiation to cardiomyocytes (hPSC-CMs). Perlecan-haploinsuffient hPSCs (HSPG2+/−) differentiate efficiently, but late-stage CMs have structural, contractile, metabolic, and ECM gene dysregulation. In keeping with this, late-stage HSPG2+/− hPSC-CMs have immature features, including reduced ⍺-actinin expression and increased glycolytic metabolism and proliferation. Moreover, perlecan-haploinsuffient engineered heart tissues have reduced tissue thickness and force generation. Conversely, hPSC-CMs grown on a perlecan-peptide substrate are enlarged and display increased nucleation, typical of hypertrophic growth. Together, perlecan appears to play the opposite role of agrin, promoting cellular maturation rather than hyperplasia and proliferation. Perlecan signaling is likely mediated via its binding to the dystroglycan complex. Targeting perlecan-dependent signaling may help reverse the phenotypic switch common to heart failure.

Published
2024
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4. Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes

Author

Johnson, Benjamin B., Cosson, Marie-Victoire, Tsansizi, Lorenza I., Holmes, Terri L., Gilmore, Tegan, Hampton, Katherine, Song, Ok-Ryul, Vo, Nguyen T.N., Nasir, Aishah, Chabronova, Alzbeta, Denning, Chris, Peffers, Mandy J., Merry, Catherine L.R., Whitelock, John, Troeberg, Linda, Rushworth, Stuart A., Bernardo, Andreia S., and Smith, James G.W.

Published
2024
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6. OPTIMIZATION OF SCALABLE rAAV PRODUCTION FOR GENE THERAPY – LEVERAGING AT-LINE AMINO ACID MEASUREMENTS FOR BIOPROCESS MODELING-DRIVEN APPROACHES

Author

Srinivasan, P., primary, Piras, G., additional, Neffling, M., additional, Josephs, J., additional, Nguyen, T.N., additional, Anderson-Czajkowski, J., additional, Miller, S., additional, Sinskey, A., additional, Braatz, R., additional, Wolfrum, J., additional, Barone, P., additional, and Springs, S., additional

Published
2024
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8. CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes

Author

Alexander Kondrashov, Nurul A.N. Mohd Yusof, Alveera Hasan, Joëlle Goulding, Thusharika Kodagoda, Duc M. Hoang, Nguyen T.N. Vo, Tony Melarangi, Nazanin Dolatshad, Julia Gorelik, Stephen J. Hill, Sian E. Harding, and Chris Denning

Subjects
Genetics, QH426-470, Cytology, QH573-671
Abstract

During normal- and patho-physiological situations, the behavior of the beta2-adrenoreceptor (β2AR) is influenced by polymorphic variants. The functional impact of such polymorphisms has been suggested from data derived from genetic association studies, in vitro experiments with primary cells, and transgenic overexpression models. However, heterogeneous genetic background and non-physiological transgene expression levels confound interpretation, leading to conflicting mechanistic conclusions. To overcome these limitations, we used CRISPR/Cas9 gene editing technology in human pluripotent stem cells (hPSCs) to create a unique suite of four isogenic homozygous variants at amino acid positions 16(G/R) and 27(G/Q), which reside in the N terminus of the β2AR. By producing cardiomyocytes from these hPSC lines, we determined that at a functional level β2AR signaling dominated over β1AR . Examining changes in beat rates and responses to isoprenaline, Gi coupling, cyclic AMP (cAMP) production, downregulation, and desensitization indicated that responses were often heightened for the GE variant, implying differential dominance of both polymorphic location and amino acid substitution. This finding was corroborated, since GE showed hypersensitivity to doxorubicin-induced cardiotoxicity relative to GQ and RQ variants. Thus, understanding the effect of β2AR polymorphisms on cardiac response to anticancer therapy may provide a route for personalized medicine and facilitate immediate clinical impact.

Published
2021
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13. Stent-Assisted Coiling in the Treatment of Unruptured Intracranial Aneurysms: A Randomized Clinical Trial

Author

Boisseau, W., primary, Darsaut, T.E., additional, Fahed, R., additional, Drake, B., additional, Lesiuk, H., additional, Rempel, J.L., additional, Gentric, J.-C., additional, Ognard, J., additional, Nico, L., additional, Iancu, D., additional, Roy, D., additional, Weill, A., additional, Chagnon, M., additional, Zehr, J., additional, Lavoie, P., additional, Nguyen, T.N., additional, and Raymond, J., additional

Published
2023
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18. Effect of thickness of polyethylene packaging and temperature on quality of solar-dried oyster mushroom (Pleurotus sajor-caju)

Author

Nguyen T.N Giang, Tran V Khai, and Nguyen M Thuy

Subjects
Ecology, Plant Science, Biochemistry, Genetics and Molecular Biology (miscellaneous), Ecology, Evolution, Behavior and Systematics
Abstract

Pleurotus sajor-caju is evaluated as an edible fungi with high nutritional and medicinal value, but fresh mushrooms are easily damaged after harvest due to many reasons. Drying methods can be taken to maintain mushroom quality, reduce losses and prolong postharvest storage time. The objective of this study was to evaluate the effects of polyethylene (PE) packaging thickness (91.70 µm; 81.30 µm and 53.50 µm), temperature (28 oC-30 oC and 3 oC-5 oC) (with air humidity of 60-62% and 76-78% respectively), to the quality of oyster mushrooms dried by solar energy, during storage. During the storage period, the total sugar and protein contents of all treatments decreased. Besides, the colour (through the difference in lightness and darkness (?L) value) and firmness of the solar-dried oyster mushrooms also decreased, so, oyster mushrooms were darkened and softened. After 6 months, the lowest protein, total sugar and lipid loss was found in PE packaging of 91.70 µm thickness at storage temperature of 3 oC-5 oC. In addition, the water activity of dried mushrooms was lower (less than 0.7), so it ensures microbiological safety.

Published
2022

19. Therapeutic Trends of Cerebrovascular Disease during the COVID-19 Pandemic and Future Perspectives

Author

Siegler, J.E., Abdalkader, M., Michel, P., and Nguyen, T.N.

Subjects
COVID-19, Epidemiology, Intracranial hemorrhages, Outcomes research, Stroke
Abstract

As of May 2022, there have been more than 400 million cases (including re-infections) of the systemic acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), and nearly 5 million deaths worldwide. Not only has the coronavirus disease 2019 (COVID-19) pandemic been responsible for diagnosis and treatment delays of a wide variety of conditions, and overwhelmed the allocation of healthcare resources, it has impacted the epidemiology and management of cerebrovascular disease. In this narrative review, we summarize the changing paradigms and latest data regarding the complex relationship between COVID-19 and cerebrovascular disease. Paradoxically, although SARS-CoV-2 has been associated with many thrombotic complications-including ischemic stroke-there have been global declines in ischemic stroke and other cerebrovascular diseases. These epidemiologic shifts may be attributed to patient avoidance of healthcare institutions due to fear of contracting the novel human coronavirus, and also related to declines in other transmissible infectious illnesses which may trigger ischemic stroke. Despite the association between SARS-CoV-2 and thrombotic events, there are inconsistent data regarding targeted antithrombotics to prevent venous and arterial events. In addition, we provide recommendations for the conduct of stroke research and clinical trial planning during the ongoing COVID-19 pandemic, and for future healthcare crises.

Published
2022

20. CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes

Author

Stephen J. Hill, Tony Melarangi, Nazanin F. Dolatshad, Alveera Hasan, Duc M. Hoang, Thusharika Kodagoda, Nguyen T.N. Vo, Sian E. Harding, Julia Gorelik, Nurul A N Mohd Yusof, Joelle Goulding, Chris Denning, Alexander Kondrashov, and British Heart Foundation

Subjects
0301 basic medicine, chemistry.chemical_classification, Genetics, lcsh:QH426-470, Cas9, lcsh:Cytology, Transgene, Biology, Amino acid, 03 medical and health sciences, lcsh:Genetics, 030104 developmental biology, 0302 clinical medicine, Genome editing, chemistry, Downregulation and upregulation, 030220 oncology & carcinogenesis, Molecular Medicine, CRISPR, Original Article, lcsh:QH573-671, Induced pluripotent stem cell, Molecular Biology, Genetic association
Abstract

During normal- and patho-physiological situations, the behavior of the beta2-adrenoreceptor (β2AR) is influenced by polymorphic variants. The functional impact of such polymorphisms has been suggested from data derived from genetic association studies, in vitro experiments with primary cells, and transgenic overexpression models. However, heterogeneous genetic background and non-physiological transgene expression levels confound interpretation, leading to conflicting mechanistic conclusions. To overcome these limitations, we used CRISPR/Cas9 gene editing technology in human pluripotent stem cells (hPSCs) to create a unique suite of four isogenic homozygous variants at amino acid positions 16(G/R) and 27(G/Q), which reside in the N terminus of the β2AR. By producing cardiomyocytes from these hPSC lines, we determined that at a functional level β2AR signaling dominated over β1AR . Examining changes in beat rates and responses to isoprenaline, Gi coupling, cyclic AMP (cAMP) production, downregulation, and desensitization indicated that responses were often heightened for the GE variant, implying differential dominance of both polymorphic location and amino acid substitution. This finding was corroborated, since GE showed hypersensitivity to doxorubicin-induced cardiotoxicity relative to GQ and RQ variants. Thus, understanding the effect of β2AR polymorphisms on cardiac response to anticancer therapy may provide a route for personalized medicine and facilitate immediate clinical impact., Graphical Abstract, During normal- and patho-physiological situations, the behavior of beta2-adrenoreceptor (β2AR) is determined by its polymorphic variants. A human-based isogenic model system represents a promising tool for systematic analysis of mechanisms of β2AR variant-mediated cellular response under normal and stressed conditions. This allows important subtleties of polymorphisms in β2AR to be unraveled.

Published
2021

25. Bloodstream Infections among HIV-Infected Outpatients, Southeast Asia

Author

Jay K. Varma, Kimberly D. McCarthy, Theerawit Tasaneeyapan, Patama Monkongdee, Michael E. Kimerling, Eng Buntheoun, Delphine Sculier, Chantary Keo, Praphan Phanuphak, Nipat Teeratakulpisarn, Nibondh Udomsantisuk, Nguyen H. Dung, Nguyen T.N. Lan, Nguyen T.B. Yen, and Kevin P. Cain

Subjects
Tuberculosis and other mycobacteria, HIV/AIDS and other retroviruses, diagnosis, bacteria, Salmonella, cryptoccoci, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

Bloodstream infections (BSIs) are a major cause of illness in HIV-infected persons. To evaluate prevalence of and risk factors for BSIs in 2,009 HIV-infected outpatients in Cambodia, Thailand, and Vietnam, we performed a single Myco/F Lytic blood culture. Fifty-eight (2.9%) had a clinically significant BSI (i.e., a blood culture positive for an organism known to be a pathogen). Mycobacterium tuberculosis accounted for 31 (54%) of all BSIs, followed by fungi (13 [22%]) and bacteria (9 [16%]). Of patients for whom data were recorded about antiretroviral therapy, 0 of 119 who had received antiretroviral therapy for >14 days had a BSI, compared with 3% of 1,801 patients who had not. In multivariate analysis, factors consistently associated with BSI were fever, low CD4+ T-lymphocyte count, abnormalities on chest radiograph, and signs or symptoms of abdominal illness. For HIV-infected outpatients with these risk factors, clinicians should place their highest priority on diagnosing tuberculosis.

Published
2010
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29. Noninvasive Angiographic Results of Clipped or Coiled Intracranial Aneurysms: An Inter- and Intraobserver Reliability Study

Author

Benomar, A., primary, Farzin, B., additional, Gevry, G., additional, Boisseau, W., additional, Roy, D., additional, Weill, A., additional, Iancu, D., additional, Guilbert, F., additional, Létourneau-Guillon, L., additional, Jacquin, G., additional, Chaalala, C., additional, Bojanowski, M.W., additional, Labidi, M., additional, Fahed, R., additional, Volders, D., additional, Nguyen, T.N., additional, Gentric, J.-C., additional, Magro, E., additional, Boulouis, G., additional, Forestier, G., additional, Hak, J.-F., additional, Ghostine, J.S., additional, Kaderali, Z., additional, Shankar, J.J., additional, Kotowski, M., additional, Darsaut, T.E., additional, and Raymond, J., additional

Published
2021
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31. Rheological Properties and Shear Induced Texture Development of Thermotopic Liquid Crystalline Polymer Melts

Author

Nguyen T.N., Geiger K., and Fritz H.-G.

Subjects
liquid crystalline polymers (lcps(, capillary rheometer, convergent flow, shear induced texture, polarized light microscopy, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract

The flow behavior of two commercial liquid crystalline polymers (LCPs), trade name Vectra B 950 and Vectra L 950 supplied by Ticona, was investigated using a capillary rheometer with a special double slit die. The pressure drops in convergent and divergent wedge passage between the two slit sections, which are influenced by the curvature strains in nematic melts, were determined and compared with the pressure drops of a conventional polystyrene (PS) with flexible polymer chains. Furthermore the extensional viscositiy was determined from the pressure drop in convergent wedge passage. The development of the shear induced texture as a function of shear rate was investigated by use of a rapidly coolable double slit die with the same gap design as the double slit die used for viscosity measurement. The structural analysis was performed using polarized light microscopy.

Published
2002
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32. CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of ?2AR in isogenic hPSC-derived cardiomyocytes

Author

Kondrashov, Alexander, Mohd Yusof, Nurul A.N., Hasan, Alveera, Kodagoda, Thusharika, Hoang, Duc M., Vo, Nguyen T.N., Melarangi, Tony, Dolatshad, Nazanin, Gorelik, Julia, Hill, Stephen J., Harding, Sian E., and Denning, Chris

Abstract

© 2020 During normal- and patho-physiological situations, the behavior of the beta2-adrenoreceptor (β2AR) is influenced by polymorphic variants. The functional impact of such polymorphisms has been suggested from data derived from genetic association studies, in vitro experiments with primary cells, and transgenic overexpression models. However, heterogeneous genetic background and non-physiological transgene expression levels confound interpretation, leading to conflicting mechanistic conclusions. To overcome these limitations, we used CRISPR/Cas9 gene editing technology in human pluripotent stem cells (hPSCs) to create a unique suite of four isogenic homozygous variants at amino acid positions 16(G/R) and 27(G/Q), which reside in the N terminus of the β2AR. By producing cardiomyocytes from these hPSC lines, we determined that at a functional level β2AR signaling dominated over β1AR. Examining changes in beat rates and responses to isoprenaline, Gi coupling, cyclic AMP (cAMP) production, downregulation, and desensitization indicated that responses were often heightened for the GE variant, implying differential dominance of both polymorphic location and amino acid substitution. This finding was corroborated, since GE showed hypersensitivity to doxorubicin-induced cardiotoxicity relative to GQ and RQ variants. Thus, understanding the effect of β2AR polymorphisms on cardiac response to anticancer therapy may provide a route for personalized medicine and facilitate immediate clinical impact. During normal- and patho-physiological situations, the behavior of beta2-adrenoreceptor (β2AR) is determined by its polymorphic variants. A human-based isogenic model system represents a promising tool for systematic analysis of mechanisms of β2AR variant-mediated cellular response under normal and stressed conditions. This allows important subtleties of polymorphisms in β2AR to be unraveled.

Published
2021

33. The use of fluorescence correlation spectroscopy to monitor cell surface β2‐adrenoceptors at low expression levels in human embryonic stem cell‐derived cardiomyocytes and fibroblasts

Author

Stephen J. Hill, Duc M. Hoang, Joelle Goulding, Nguyen T.N. Vo, Alexander Kondrashov, Stephen J. Briddon, Chris Denning, Sarah J. Mistry, Carl W. White, and Tony Melarangi

Subjects
0301 basic medicine, Cell, Biochemistry, law.invention, 03 medical and health sciences, 0302 clinical medicine, Cell surface receptor, Confocal microscopy, law, Genetics, medicine, Humans, Myocytes, Cardiac, Progenitor cell, Primary cell, Adrenergic beta-2 Receptor Agonists, Molecular Biology, Embryonic Stem Cells, Fluorescent Dyes, Chemistry, HEK 293 cells, Membrane Proteins, Cell Differentiation, Fibroblasts, Propranolol, Embryonic stem cell, Cell biology, HEK293 Cells, Spectrometry, Fluorescence, 030104 developmental biology, medicine.anatomical_structure, Gene Expression Regulation, Cell culture, Receptors, Adrenergic, beta-2, 030217 neurology & neurosurgery, Biotechnology
Abstract

The importance of cell phenotype in determining the molecular mechanisms underlying β2 -adrenoceptor (β2AR) function has been noted previously when comparing responses in primary cells and recombinant model cell lines. Here, we have generated haplotype-specific SNAP-tagged β2AR human embryonic stem (ES) cell lines and applied fluorescence correlation spectroscopy (FCS) to study cell surface receptors in progenitor cells and in differentiated fibroblasts and cardiomyocytes. FCS was able to quantify SNAP-tagged β2AR number and diffusion in both ES-derived cardiomyocytes and CRISPR/Cas9 genome-edited HEK293T cells, where the expression level was too low to detect using standard confocal microscopy. These studies demonstrate the power of FCS in investigating cell surface β2ARs at the very low expression levels often seen in endogenously expressing cells. Furthermore, the use of ES cell technology in combination with FCS allowed us to demonstrate that cell surface β2ARs internalize in response to formoterol-stimulation in ES progenitor cells but not following their differentiation into ES-derived fibroblasts. This indicates that the process of agonist-induced receptor internalization is strongly influenced by cell phenotype and this may have important implications for drug treatment with long-acting β2AR agonists.

Published
2021

34. Decline in subarachnoid haemorrhage volumes associated with the first wave of the COVID-19 pandemic

Author

Nguyen, T.N. Haussen, D.C. Qureshi, M.M. Yamagami, H. Fujinaka, T. Mansour, O.Y. Abdalkader, M. Frankel, M. Qiu, Z. Taylor, A. Lylyk, P. Eker, O.F. Mechtouff, L. Piotin, M. Lima, F.O. Mont'Alverne, F. Izzath, W. Sakai, N. Mohammaden, M. Al-Bayati, A.R. Renieri, L. Mangiafico, S. Ozretic, D. Chalumeau, V. Ahmad, S. Rashid, U. Hussain, S.I. John, S. Griffin, E. Thornton, J. Fiorot, J.A. Rivera, R. Hammami, N. Cervantes-Arslanian, A.M. Dasenbrock, H.H. Vu, H.L. Nguyen, V.Q. Hetts, S. Bourcier, R. Guile, R. Walker, M. Sharma, M. Frei, D. Jabbour, P. Herial, N. Al-Mufti, F. Ozdemir, A.O. Aykac, O. Gandhi, D. Chugh, C. Matouk, C. Lavoie, P. Edgell, R. Beer-Furlan, A. Chen, M. Killer-Oberpfalzer, M. Pereira, V.M. Nicholson, P. Huded, V. Ohara, N. Watanabe, D. Shin, D.H. Magalhaes, P.S.C. Kikano, R. Ortega-Gutierrez, S. Farooqui, M. Abou-Hamden, A. Amano, T. Yamamoto, R. Weeks, A. Cora, E.A. Sivan-Hoffmann, R. Crosa, R. Möhlenbruch, M. Nagel, S. Al-Jehani, H. Sheth, S.A. Rivera, V.S.L. Siegler, J.E. Sani, A.F. Puri, A.S. Kuhn, A.L. Bernava, G. Machi, P. Abud, D.G. Pontes-Neto, O.M. Wakhloo, A.K. Voetsch, B. Raz, E. Yaghi, S. Mehta, B.P. Kimura, N. Murakami, M. Lee, J.S. Hong, J.M. Fahed, R. Walker, G. Hagashi, E. Cordina, S.M. Roh, H.G. Wong, K. Arenillas, J.F. Martinez-Galdamez, M. Blasco, J. Vasquez, A.R. Fonseca, L. Silva, M.L. Wu, T.Y. John, S. Brehm, A. Psychogios, M. Mack, W.J. Tenser, M. Todaka, T. Fujimura, M. Novakovic, R. Deguchi, J. Sugiura, Y. Tokimura, H. Khatri, R. Kelly, M. Peeling, L. Murayama, Y. Winters, H.S. Wong, J. Teleb, M. Payne, J. Fukuda, H. Miyake, K. Shimbo, J. Sugimura, Y. Uno, M. Takenobu, Y. Matsumaru, Y. Yamada, S. Kono, R. Kanamaru, T. Morimoto, M. Iida, J. Saini, V. Yavagal, D. Bushnaq, S. Huang, W. Linfante, I. Kirmani, J. Liebeskind, D.S. Szeder, V. Shah, R. Devlin, T.G. Birnbaum, L. Luo, J. Churojana, A. Masoud, H.E. Lopez, C.Y. Steinfort, B. Ma, A. Hassan, A.E. Al Hashmi, A. McDermott, M. Mokin, M. Chebl, A. Kargiotis, O. Tsivgoulis, G. Morris, J.G. Eskey, C.J. Thon, J. Rebello, L. Altschul, D. Cornett, O. Singh, V. Pandian, J. Kulkarni, A. Lavados, P.M. Olavarria, V.V. Todo, K. Yamamoto, Y. Silva, G.S. Geyik, S. Johann, J. Multani, S. Kaliaev, A. Sonoda, K. Hashimoto, H. Alhazzani, A. Chung, D.Y. Mayer, S.A. Fifi, J.T. Hill, M.D. Zhang, H. Yuan, Z. Shang, X. Castonguay, A.C. Gupta, R. Jovin, T.G. Raymond, J. Zaidat, O.O. Nogueira, R.G.

Subjects
cardiovascular diseases, nervous system diseases
Abstract

Background During the COVID-19 pandemic, decreased volumes of stroke admissions and mechanical thrombectomy were reported. The study's objective was to examine whether subarachnoid haemorrhage (SAH) hospitalisations and ruptured aneurysm coiling interventions demonstrated similar declines. Methods We conducted a cross-sectional, retrospective, observational study across 6 continents, 37 countries and 140 comprehensive stroke centres. Patients with the diagnosis of SAH, aneurysmal SAH, ruptured aneurysm coiling interventions and COVID-19 were identified by prospective aneurysm databases or by International Classification of Diseases, 10th Revision, codes. The 3-month cumulative volume, monthly volumes for SAH hospitalisations and ruptured aneurysm coiling procedures were compared for the period before (1 year and immediately before) and during the pandemic, defined as 1 March-31 May 2020. The prior 1-year control period (1 March-31 May 2019) was obtained to account for seasonal variation. Findings There was a significant decline in SAH hospitalisations, with 2044 admissions in the 3 months immediately before and 1585 admissions during the pandemic, representing a relative decline of 22.5% (95% CI -24.3% to -20.7%, p

Published
2021

35. Global Impact of COVID-19 on Stroke Care and IV Thrombolysis

Author

Nogueira, R.G. Qureshi, M.M. Abdalkader, M. Martins, S.O. Yamagami, H. Qiu, Z. Mansour, O.Y. Sathya, A. Czlonkowska, A. Tsivgoulis, G. Aguiar de Sousa, D. Demeestere, J. Mikulik, R. Vanacker, P. Siegler, J.E. Kõrv, J. Biller, J. Liang, C.W. Sangha, N.S. Zha, A.M. Czap, A.L. Holmstedt, C.A. Turan, T.N. Ntaios, G. Malhotra, K. Tayal, A. Loochtan, A. Ranta, A. Mistry, E.A. Alexandrov, A.W. Huang, D.Y. Yaghi, S. Raz, E. Sheth, S.A. Mohammaden, M.H. Frankel, M. Bila Lamou, E.G. Aref, H.M. Elbassiouny, A. Hassan, F. Menecie, T. Mustafa, W. Shokri, H.M. Roushdy, T. Sarfo, F.S. Alabi, T.O. Arabambi, B. Nwazor, E.O. Sunmonu, T.A. Wahab, K. Yaria, J. Mohammed, H.H. Adebayo, P.B. Riahi, A.D. Sassi, S.B. Gwaunza, L. Ngwende, G.W. Sahakyan, D. Rahman, A. Ai, Z. Bai, F. Duan, Z. Hao, Y. Huang, W. Li, G. Li, W. Liu, G. Luo, J. Shang, X. Sui, Y. Tian, L. Wen, H. Wu, B. Yan, Y. Yuan, Z. Zhang, H. Zhang, J. Zhao, W. Zi, W. Leung, T.W. Chugh, C. Huded, V. Menon, B. Pandian, J.D. Sylaja, P.N. Usman, F.S. Farhoudi, M. Hokmabadi, E.S. Horev, A. Reznik, A. Sivan Hoffmann, R. Ohara, N. Sakai, N. Watanabe, D. Yamamoto, R. Doijiri, R. Tokuda, N. Yamada, T. Terasaki, T. Yazawa, Y. Uwatoko, T. Dembo, T. Shimizu, H. Sugiura, Y. Miyashita, F. Fukuda, H. Miyake, K. Shimbo, J. Sugimura, Y. Yagita, Y. Takenobu, Y. Matsumaru, Y. Yamada, S. Kono, R. Kanamaru, T. Yamazaki, H. Sakaguchi, M. Todo, K. Yamamoto, N. Sonoda, K. Yoshida, T. Hashimoto, H. Nakahara, I. Kondybayeva, A. Faizullina, K. Kamenova, S. Zhanuzakov, M. Baek, J.-H. Hwang, Y. Lee, J.S. Lee, S.B. Moon, J. Park, H. Seo, J.H. Seo, K.-D. Sohn, S.I. Young, C.J. Ahdab, R. Wan Zaidi, W.A. Aziz, Z.A. Basri, H.B. Chung, L.W. Ibrahim, A.B. Ibrahim, K.A. Looi, I. Tan, W.Y. Yahya, N.W. Groppa, S. Leahu, P. Al Hashmi, A.M. Imam, Y.Z. Akhtar, N. Pineda-Franks, M.C. Co, C.O. Kandyba, D. Alhazzani, A. Al-Jehani, H. Tham, C.H. Mamauag, M.J. Venketasubramanian, N. Chen, C.-H. Tang, S.-C. Churojana, A. Akil, E. Aykaç, Ö. Ozdemir, A.O. Giray, S. Hussain, S.I. John, S. Le Vu, H. Tran, A.D. Nguyen, H.H. Nhu Pham, T. Nguyen, T.H. Nguyen, T.Q. Gattringer, T. Enzinger, C. Killer-Oberpfalzer, M. Bellante, F. De Blauwe, S. Vanhooren, G. De Raedt, S. Dusart, A. Lemmens, R. Ligot, N. Pierre Rutgers, M. Yperzeele, L. Alexiev, F. Sakelarova, T. Bedeković, M.R. Budincevic, H. Cindric, I. Hucika, Z. Ozretic, D. Saric, M.S. Pfeifer, F. Karpowic, I. Cernik, D. Sramek, M. Skoda, M. Hlavacova, H. Klecka, L. Koutny, M. Vaclavik, D. Skoda, O. Fiksa, J. Hanelova, K. Nevsimalova, M. Rezek, R. Prochazka, P. Krejstova, G. Neumann, J. Vachova, M. Brzezanski, H. Hlinovsky, D. Tenora, D. Jura, R. Jurák, L. Novak, J. Novak, A. Topinka, Z. Fibrich, P. Sobolova, H. Volny, O. Krarup Christensen, H. Drenck, N. Klingenberg Iversen, H. Simonsen, C.Z. Truelsen, T.C. Wienecke, T. Vibo, R. Gross-Paju, K. Toomsoo, T. Antsov, K. Caparros, F. Cordonnier, C. Dan, M. Faucheux, J.-M. Mechtouff, L. Eker, O. Lesaine, E. Ondze, B. Peres, R. Pico, F. Piotin, M. Pop, R. Rouanet, F. Gubeladze, T. Khinikadze, M. Lobjanidze, N. Tsiskaridze, A. Nagel, S. Ringleb, P.A. Rosenkranz, M. Schmidt, H. Sedghi, A. Siepmann, T. Szabo, K. Thomalla, G. Palaiodimou, L. Sagris, D. Kargiotis, O. Klivenyi, P. Szapary, L. Tarkanyi, G. Adami, A. Bandini, F. Calabresi, P. Frisullo, G. Renieri, L. Sangalli, D. Pirson, A. Uyttenboogaart, M. van den Wijngaard, I. Kristoffersen, E.S. Brola, W. Fudala, M. Horoch-Lyszczarek, E. Karlinski, M. Kazmierski, R. Kram, P. Rogoziewicz, M. Kaczorowski, R. Luchowski, P. Sienkiewicz-Jarosz, H. Sobolewski, P. Fryze, W. Wisniewska, A. Wiszniewska, M. Ferreira, P. Ferreira, P. Fonseca, L. Marto, J.P. Pinho E Melo, T. Nunes, A.P. Rodrigues, M. Tedim Cruz, V. Falup-Pecurariu, C. Krastev, G. Mako, M. de Leciñana, M.A. Arenillas, J.F. Ayo-Martin, O. Cruz Culebras, A. Tejedor, E.D. Montaner, J. Pérez-Sánchez, S. Tola Arribas, M.A. Rodriguez Vasquez, A. Mayza, M. Bernava, G. Brehm, A. Machi, P. Fischer, U. Gralla, J. Michel, P.L. Psychogios, M.-N. Strambo, D. Banerjee, S. Krishnan, K. Kwan, J. Butt, A. Catanese, L. Demchuk, A.M. Field, T. Haynes, J. Hill, M.D. Khosravani, H. Mackey, A. Pikula, A. Saposnik, G. Scott, C.A. Shoamanesh, A. Shuaib, A. Yip, S. Barboza, M.A. Barrientos, J.D. Portillo Rivera, L.I. Gongora-Rivera, F. Novarro-Escudero, N. Blanco, A. Abraham, M. Alsbrook, D. Altschul, D. Alvarado-Ortiz, A.J. Bach, I. Badruddin, A. Barazangi, N. Brereton, C. Castonguay, A. Chaturvedi, S. Chaudry, S.A. Choe, H. Choi, J.H. Dharmadhikari, S. Desai, K. Devlin, T.G. Doss, V.T. Edgell, R. Etherton, M. Farooqui, M. Frei, D. Gandhi, D. Grigoryan, M. Gupta, R. Hassan, A.E. Helenius, J. Kaliaev, A. Kaushal, R. Khandelwal, P. Khawaja, A.M. Khoury, N.N. Kim, B.S. Kleindorfer, D.O. Koyfman, F. Lee, V.H. Leung, L.Y. Linares, G. Linfante, I. Lutsep, H.L. Macdougall, L. Male, S. Malik, A.M. Masoud, H. McDermott, M. Mehta, B.P. Min, J. Mittal, M. Morris, J.G. Multani, S.S. Nahab, F. Nalleballe, K. Nguyen, C.B. Novakovic-White, R. Ortega-Gutierrez, S. Rahangdale, R.H. Ramakrishnan, P. Romero, J.R. Rost, N. Rothstein, A. Ruland, S. Shah, R. Sharma, M. Silver, B. Simmons, M. Singh, A. Starosciak, A.K. Strasser, S.L. Szeder, V. Teleb, M. Tsai, J.P. Voetsch, B. Balaguera, O. Pujol Lereis, V.A. Luraschi, A. Almeida, M.S. Cardoso, F.B. Conforto, A. De Deus Silva, L. Varrone Giacomini, L. Oliveira Lima, F. Longo, A.L. Magalhães, P.S.C. Martins, R.T. Mont'alverne, F. Mora Cuervo, D.L. Costa Rebello, L. Valler, L. Zetola, V.F. Lavados, P.M. Navia, V. Olavarría, V.V. Almeida Toro, J.M. Amaya, P.F.R. Bayona, H. Corredor, A. Rivera Ordonez, C.E. Mantilla Barbosa, D.K. Lara, O. Patiño, M.R. Diaz Escobar, L.F. Dejesus Melgarejo Fariña, D.E. Cardozo Villamayor, A. Zelaya Zarza, A.J. Barrientos Iman, D.M. Rodriguez Kadota, L. Campbell, B. Hankey, G.J. Hair, C. Kleinig, T. Ma, A. Tomazini Martins, R. Sahathevan, R. Thijs, V. Salazar, D. Yuan-Hao Wu, T. Haussen, D.C. Liebeskind, D. Yavagal, D.R. Jovin, T.G. Zaidat, O.O. Nguyen, T.N. SVIN COVID-19 Global Stroke Registry SVIN COVID-19 Global Stroke Registry

Abstract

OBJECTIVE: To measure the global impact of COVID-19 pandemic on volumes of IV thrombolysis (IVT), IVT transfers, and stroke hospitalizations over 4 months at the height of the pandemic (March 1 to June 30, 2020) compared with 2 control 4-month periods. METHODS: We conducted a cross-sectional, observational, retrospective study across 6 continents, 70 countries, and 457 stroke centers. Diagnoses were identified by their ICD-10 codes or classifications in stroke databases. RESULTS: There were 91,373 stroke admissions in the 4 months immediately before compared to 80,894 admissions during the pandemic months, representing an 11.5% (95% confidence interval [CI] -11.7 to -11.3, p < 0.0001) decline. There were 13,334 IVT therapies in the 4 months preceding compared to 11,570 procedures during the pandemic, representing a 13.2% (95% CI -13.8 to -12.7, p < 0.0001) drop. Interfacility IVT transfers decreased from 1,337 to 1,178, or an 11.9% decrease (95% CI -13.7 to -10.3, p = 0.001). Recovery of stroke hospitalization volume (9.5%, 95% CI 9.2-9.8, p < 0.0001) was noted over the 2 later (May, June) vs the 2 earlier (March, April) pandemic months. There was a 1.48% stroke rate across 119,967 COVID-19 hospitalizations. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was noted in 3.3% (1,722/52,026) of all stroke admissions. CONCLUSIONS: The COVID-19 pandemic was associated with a global decline in the volume of stroke hospitalizations, IVT, and interfacility IVT transfers. Primary stroke centers and centers with higher COVID-19 inpatient volumes experienced steeper declines. Recovery of stroke hospitalization was noted in the later pandemic months. © 2021 American Academy of Neurology.

Published
2021

39. The Mycobacterium tuberculosis Beijing genotype does not affect tuberculosis treatment failure in Vietnam

Author

Buu, Tran N., Huyen, Mai N.T., van Soolingen, Dick, Lan, Nguyen T.N., Quy, Hoang T., Tiemersma, Edine W., Borgdorff, Martien W., and Cobelens, Frank G.J.

Subjects
Mycobacterium tuberculosis -- Identification and classification, Mycobacterium tuberculosis -- Genetic aspects, Mycobacterium tuberculosis -- Research, Tuberculosis -- Care and treatment, Tuberculosis -- Genetic aspects, Tuberculosis -- Patient outcomes, Tuberculosis -- Research, Drug resistance in microorganisms -- Risk factors, Drug resistance in microorganisms -- Research, Genotype -- Research, Health, Health care industry
Published
2010

40. Bloodstream infections among HIV-infected outpatients, Southeast Asia

Author

Varma, Jay K., McCarthy, Kimberly D., Tasaneeyapan, Theerawit, Monkongdee, Patama, Kimerling, Michael E., Buntheoun, Eng, Sculier, Delphine, Keo, Chantary, Phanuphak, Praphan, Teeratakulpisarn, Nipat, Udomsantisuk, Nibondh, Dung, Nguyen H., Lan, Nguyen T.N., Yen, Nguyen T.B., and Cain, Kevin P.

Subjects
Blood -- Medical examination, Antiviral agents -- Analysis, Tuberculosis -- Care and treatment -- Risk factors -- Analysis, Infection -- Care and treatment -- Risk factors -- Analysis, HIV patients -- Care and treatment -- Analysis, HIV infection -- Care and treatment -- Risk factors -- Analysis, Health
Abstract

Bloodstream infections (BSIs) are a major cause of illness in HIV-infected persons. A series of studies, most of which were conducted in sub-Saharan Africa during the 1990s, demonstrated a high [...]

Published
2010
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41. The use of fluorescence correlation spectroscopy to monitor cell surface β2‐adrenoceptors at low expression levels in human embryonic stem cell‐derived cardiomyocytes and fibroblasts

Author

Goulding, Joëlle, primary, Kondrashov, Alexander, additional, Mistry, Sarah J., additional, Melarangi, Tony, additional, Vo, Nguyen T.N., additional, Hoang, Duc M., additional, White, Carl W., additional, Denning, Chris, additional, Briddon, Stephen J., additional, and Hill, Stephen J., additional

Published
2021
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42. CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes

Author

Kondrashov, Alexander, primary, Mohd Yusof, Nurul A.N., additional, Hasan, Alveera, additional, Goulding, Joëlle, additional, Kodagoda, Thusharika, additional, Hoang, Duc M., additional, Vo, Nguyen T.N., additional, Melarangi, Tony, additional, Dolatshad, Nazanin, additional, Gorelik, Julia, additional, Hill, Stephen J., additional, Harding, Sian E., additional, and Denning, Chris, additional

Published
2021
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43. Reliability of the Diagnosis of Cerebral Vasospasm Using Catheter Cerebral Angiography: A Systematic Review and Inter- and Intraobserver Study

Author

Darsaut, T.E., primary, Derksen, C., additional, Farzin, B., additional, Keough, M.B., additional, Fahed, R., additional, Boisseau, W., additional, Letourneau-Guillon, L., additional, Januel, A.-C., additional, Weill, A., additional, Roy, D., additional, Nguyen, T.N., additional, Finitsis, S., additional, Gentric, J.-C., additional, Volders, D., additional, Carlson, A., additional, Chow, M.M., additional, O’Kelly, C., additional, Rempel, J.L., additional, Ashforth, R.A., additional, Chagnon, M., additional, Zehr, J., additional, Findlay, J.M., additional, Gevry, G., additional, and Raymond, J., additional

Published
2021
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46. Anion/Cation Layers at Electrified Interfaces: A Comprehensive STM, XRD and XPS Case Study

Author

Duc T. Pham, Hubert Keller, Stephan Breuer, Sascha Huemann, Nguyen T.N. Hai, Caroline Zoerlein, Klaus Wandelt, and Peter Broekmann

Subjects
In situ stm, Sxps, Sxrd, Copper electrochemistry, Electron transfer reaction, Surface phase transition, Chemistry, QD1-999
Abstract

Charged organic adsorbates play an important role in a number of electrochemical reactions, e.g. as additives for metal plating relevant for device fabrication in the semiconductor industry. Fundamental investigations are mandatory in order to acquire profound knowledge of the structural and electronic properties of these layers parallel and perpendicular to the surface, and to finally achieve a deeper mechanistic understanding of the kinetics of involved charge transfer reactions taking place at these complex metal/organic/electrolyte interfaces. A key structural motif of these interfaces consists in 'paired' (inorganic)anion/(organic)cation layers that can have an enormous stability even during an ongoing charge transfer reaction. In this contribution we present and discuss a selected case study on the co-adsorption of halide anions and cationic organic molecules that exhibit a pronounced redox activity. It will be demonstrated that their phase behavior at the interface crucially depends on both their particular redox-state and the surface concentration of the halide counter ions. The subtle balance between adsorbate–adsorbate and adsorbate–substrate interaction of the poly-cationic organic layer can be carefully controlled by potential dependent anion adsorption and desorption processes through the organic layer. This process can be followed by in situ high-resolution scanning tunnelling microscopy, while additional information about the structural and chemical state of the respective phase is obtained from in situ X-ray diffraction and ex situ photoelectron spectroscopy.

Published
2009
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47. Public-private mix for DOTS implementation: what makes it work?

Author

Lonnroth, Knut, Uplekar, Mukund, Arora, Vijay K., Juvekar, Sanjay, Lan, Nguyen T.N., Mwaniki, David, and Pathania, Vikram

Subjects
Developing countries -- Social aspects, Medical care -- Evaluation, Medical care -- United Kingdom
Abstract

Objective To compare processes and outcomes of four public-private mix (PPM) projects on DOTS implementation for tuberculosis (TB) control in New Delhi, India; Ho Chi Minh City, Viet Nam; Nairobi, Kenya; and Pune, India. Methods Cross-project analysis of secondary data from separate project evaluations was used. Differences among PPM project sites in impact on TB control (change in case detection, treatment outcomes and equity in access) were correlated with differences in chosen intervention strategies and structural conditions. Findings The analysis suggests that an effective intervention package should include the following provider-side components: (1) orienting private providers (PPs) and the staff of the national TB programme (NTP); (2) improving the referral and information system through simple practical tools; (3) the NTP adequately supervising and monitoring PPs; and (4) the NTP providing free anti-TB drugs to patients treated in the private sector. Conclusion Getting such an intervention package to work requires that the NTP be strongly committed to supporting, supervising and evaluating PPM projects. Further, using a local nongovernmental organization or a medical association as an intermediary may facilitate collaboration. Investing time and effort to ensure that sufficient dialogue takes place among all stakeholders is important to help build trust and achieve a high level of agreement. Keywords Tuberculosis Pulmonary/drug therapy; Antitubercular agents/supply and distribution; Private sector/utilization; Public sector; Directly observed therapy/utilization; National health programs/organization and administration; Health plan implementation; Intersectoral cooperation; Outcome and process assessment (Health care); Comparative study; India; Kenya; Viet Nam (source: MESH, NLM). Mots cles Tuberculose pulmonaire/chimiotherapie; Antituberculeux/ressources et distribution; Secteur prive/utilisation; Secteur public; Therapie sous observation directe/utilisation; Programme national sante/organisation et administration; Mise en oeuvre plan sanitaire; Cooperation intersectorielle; Evaluation resultats et methodes (Soins); Etude comparative; Inde; Kenya; Viet Nam (source: MESH, INSERM). Palabras clave Tuberculosis pulmonar/quimioterapia; Agentes antituberculosos/provision y distribucion; Sector privado/utilizacion; Sector publico; Terapia por observacion directa/utilizacion; Programas nacionales de salud/organizacion y administracion; Implementacion de plan de salud; Cooperacion intersectorial; Evaluacion de procesos y resultados (Atencion de salud); Estudio comparativo; India; Kenya; Viet Nam (fuente: DeCS, BIREME). [TEXT NOT REPRODUCIBLE IN ASCII.], Resume Partenariat public-prive pour la mise en ouvre de la strategie DOTS : quels sont les facteurs de succes? Objectif Comparer les processus de developpement et de realisation et les [...]

Published
2004

48. Does Increasing Packing Density Using Larger Caliber Coils Improve Angiographic Results of Embolization of Intracranial Aneurysms at 1 Year: A Randomized Trial

Author

Raymond, J., primary, Ghostine, J., additional, van Adel, B.A., additional, Shankar, J.J.S., additional, Iancu, D., additional, Mitha, A.P., additional, Kvamme, P., additional, Turner, R.D., additional, Turk, A., additional, Mendes-Pereira, V., additional, Carpenter, J.S., additional, Boo, S., additional, Evans, A., additional, Woo, H.H., additional, Fiorella, D., additional, Alaraj, A., additional, Roy, D., additional, Weill, A., additional, Lavoie, P., additional, Chagnon, M., additional, Nguyen, T.N., additional, Rempel, J.L., additional, and Darsaut, T.E., additional

Published
2020
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49. Challenges and Imaging Applications of Lanthanide(III)-Based Metallacrowns Emitting in the Near-Infrared Range

Author

Eliseeva, S.V., Lutter, J.C., Nguyen, T.N., Martinic, I., Collet, G., Sobilo, J., Lerondel, S., Pecoraro, V.L., Petoud, S., Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Centre d'imagerie du Petit Animal, Centre National de la Recherche Scientifique (CNRS), Cente d'imagerie du Petit Animal, and Frapart, Isabelle

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

50. Near-infrared emitting lanthanide probes for optical imaging agents with different sizes: small molecules, metallacrowns and MOF nanomaterials

Author

Petoud, S., Eliseeva, S.V., Collet, G., Martinic, I., Lutter, J.C., Nguyen, T.N., Delatouche, R., Chopin, N., Suzenet, Franck, Pecoraro, V.L., Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Synthèse Organique (E5), Institut de Chimie des Milieux et Matériaux de Poitiers (IC2MP), Université de Poitiers-Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre Léon Bérard [Lyon], Institut de Chimie Organique et Analytique (ICOA), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

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