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Your search keyword '"Nguyen, Vivian V.T."' showing total 7 results
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7 results on '"Nguyen, Vivian V.T."'

2. Inter-laboratory multiplex bead-based surface protein profiling of MSC-derived EV preparations identifies MSC-EV surface marker signatures

Author

Nguyen, Vivian V.T., Welsh, Joshua A., Tertel, Tobias, Choo, Andre, van de Wakker, Simonides I., Defourny, Kyra A.Y., Giebel, Bernd, Vader, Pieter, Padmanabhan, Jayanthi, Lim, Sai Kiang, Nolte-'t Hoen, Esther N.M., Verhaar, Marianne C., Bostancioglu, R. Beklem, Zickler, Antje M., Hong, Jia Mei, Jones, Jennifer C., EL Andaloussi, Samir, van Balkom, Bas W.M., Görgens, André, Nguyen, Vivian V.T., Welsh, Joshua A., Tertel, Tobias, Choo, Andre, van de Wakker, Simonides I., Defourny, Kyra A.Y., Giebel, Bernd, Vader, Pieter, Padmanabhan, Jayanthi, Lim, Sai Kiang, Nolte-'t Hoen, Esther N.M., Verhaar, Marianne C., Bostancioglu, R. Beklem, Zickler, Antje M., Hong, Jia Mei, Jones, Jennifer C., EL Andaloussi, Samir, van Balkom, Bas W.M., and Görgens, André

Abstract

Mesenchymal stromal cells (MSCs) are promising regenerative therapeutics that primarily exert their effects through secreted extracellular vesicles (EVs). These EVs – being small and non-living – are easier to handle and possess advantages over cellular products. Consequently, the therapeutic potential of MSC-EVs is increasingly investigated. However, due to variations in MSC-EV manufacturing strategies, MSC-EV products should be considered as highly diverse. Moreover, the diverse array of EV characterisation technologies used for MSC-EV characterisation further complicates reliable interlaboratory comparisons of published data. Consequently, this study aimed to establish a common method that can easily be used by various MSC-EV researchers to characterise MSC-EV preparations to facilitate interlaboratory comparisons. To this end, we conducted a comprehensive inter-laboratory assessment using a novel multiplex bead-based EV flow cytometry assay panel. This assessment involved 11 different MSC-EV products from five laboratories with varying MSC sources, culture conditions, and EV preparation methods. Through this assay panel covering a range of mostly MSC-related markers, we identified a set of cell surface markers consistently positive (CD44, CD73 and CD105) or negative (CD11b, CD45 and CD197) on EVs of all explored MSC-EV preparations. Hierarchical clustering analysis revealed distinct surface marker profiles associated with specific preparation processes and laboratory conditions. We propose CD73, CD105 and CD44 as robust positive markers for minimally identifying MSC-derived EVs and CD11b, CD14, CD19, CD45 and CD79 as reliable negative markers. Additionally, we highlight the influence of culture medium components, particularly human platelet lysate, on EV surface marker profiles, underscoring the influence of culture conditions on resulting EV products. This standardisable approach for MSC-EV surface marker profiling offers a tool for routine characterisation

Published
2024

3. Inter-laboratory multiplex bead-based surface protein profiling of MSC-derived EV preparations identifies MSC-EV surface marker signatures

Author

Cardiologie, CDL Staf Research, CDL Cluster Onderzoek en Onderwijs, Circulatory Health, Zorgeenheid Vaatchirurgie Medisch, UMC Utrecht Holding, Nefro Vasculaire Geneeskunde, Regenerative Medicine and Stem Cells, MS Nefrologie, Nguyen, Vivian V.T., Welsh, Joshua A., Tertel, Tobias, Choo, Andre, van de Wakker, Simonides I., Defourny, Kyra A.Y., Giebel, Bernd, Vader, Pieter, Padmanabhan, Jayanthi, Lim, Sai Kiang, Nolte-'t Hoen, Esther N.M., Verhaar, Marianne C., Bostancioglu, R. Beklem, Zickler, Antje M., Hong, Jia Mei, Jones, Jennifer C., EL Andaloussi, Samir, van Balkom, Bas W.M., Görgens, André, Cardiologie, CDL Staf Research, CDL Cluster Onderzoek en Onderwijs, Circulatory Health, Zorgeenheid Vaatchirurgie Medisch, UMC Utrecht Holding, Nefro Vasculaire Geneeskunde, Regenerative Medicine and Stem Cells, MS Nefrologie, Nguyen, Vivian V.T., Welsh, Joshua A., Tertel, Tobias, Choo, Andre, van de Wakker, Simonides I., Defourny, Kyra A.Y., Giebel, Bernd, Vader, Pieter, Padmanabhan, Jayanthi, Lim, Sai Kiang, Nolte-'t Hoen, Esther N.M., Verhaar, Marianne C., Bostancioglu, R. Beklem, Zickler, Antje M., Hong, Jia Mei, Jones, Jennifer C., EL Andaloussi, Samir, van Balkom, Bas W.M., and Görgens, André

Published
2024

4. A human kidney and liver organoid-based multi-organ-on-a-chip model to study the therapeutic effects and biodistribution of mesenchymal stromal cell-derived extracellular vesicles

Author

MS Nefrologie, Nefro Vasculaire Geneeskunde, UMC Utrecht Holding, Circulatory Health, Regenerative Medicine and Stem Cells, Nguyen, Vivian V.T., Ye, Shicheng, Gkouzioti, Vasiliki, van Wolferen, Monique E., Yengej, Fjodor Yousef, Melkert, Dennis, Siti, Sofia, de Jong, Bart, Besseling, Paul J., Spee, Bart, van der Laan, Luc J.W., Horland, Reyk, Verhaar, Marianne C., van Balkom, Bas W.M., MS Nefrologie, Nefro Vasculaire Geneeskunde, UMC Utrecht Holding, Circulatory Health, Regenerative Medicine and Stem Cells, Nguyen, Vivian V.T., Ye, Shicheng, Gkouzioti, Vasiliki, van Wolferen, Monique E., Yengej, Fjodor Yousef, Melkert, Dennis, Siti, Sofia, de Jong, Bart, Besseling, Paul J., Spee, Bart, van der Laan, Luc J.W., Horland, Reyk, Verhaar, Marianne C., and van Balkom, Bas W.M.

Published
2022

5. Stem cells, organoids, and organ-on-a-chip models for personalized in vitro drug testing

Author

MS Nefrologie, Nefro Vasculaire Geneeskunde, Circulatory Health, Regenerative Medicine and Stem Cells, van Berlo, Damiën, Nguyen, Vivian V.T., Gkouzioti, Vasiliki, Leineweber, Kirsten, Verhaar, Marianne C., van Balkom, Bas W.M., MS Nefrologie, Nefro Vasculaire Geneeskunde, Circulatory Health, Regenerative Medicine and Stem Cells, van Berlo, Damiën, Nguyen, Vivian V.T., Gkouzioti, Vasiliki, Leineweber, Kirsten, Verhaar, Marianne C., and van Balkom, Bas W.M.

Published
2021

7. Stem cells, organoids, and organ-on-a-chip models for personalized in vitrodrug testing

Author

van Berlo, Damiën, Nguyen, Vivian V.T., Gkouzioti, Vasiliki, Leineweber, Kirsten, Verhaar, Marianne C., and van Balkom, Bas W.M.

Abstract

Breakthroughs in stem cell biology and microfluidics technology have opened doors to in vitroscreening platforms for personalized testing of safety (pharmaceuticals, nutrients, chemicals) and efficacy (pharmaceuticals, nutraceuticals). Major breakthrough technologies include development of induced pluripotent stem cells, the development of induced pluripotent stem cell-derived organoids and adult stem cell-derived organoids, and the generation of organ-on-a-chip and multi-organ-on-a-chip models to mimic human physiology in vitro. These technologies are highly complementary and offer tremendous potential for improved efficiency in drug development and chemical safety testing. In the current review, we will provide an overview of recent advances in in vitromodeling for personalized drug testing based on stem cell and organ-on-a-chip technologies and illustrate how these developments will eventually lead to the replacement of animal testing. Particular focus will be on multi-organ-on-chip human disease models, which have the potential to be the gold standard of the future for the investigation of safety, toxicity, and efficacy of newly developed medicines.

Published
2021
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