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Caijun Dai,1,* Huihua Qiu,2,* Qiqi Huang,2,* Pinglang Hu,2 Xianchai Hong,2 Junwei Tu,1 Qiangli Xie,2 Haiyan Li,2 Wenwei Ren,2 Shuhong Ni,1 Fujian Chen3 1Jinhua Municipal Central Hospital, Jinhua 321000, China; 2The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China; 3Anji County People’s Hospital, Huzhou 313300, China *These authors contributed equally to this work Purpose: Night shift is associated with adverse physical and psychological health outcomes such as poor sleep quality and depressive symptoms. We aimed to compare sleep quality as well as depressive symptoms in nurses working night shifts to those working day shifts only and explore the association between sleep quality and depressive symptoms among nurses. Patients and methods: Eight hundred sixty-five nurses were enrolled in the current study. Sleep quality and depressive symptoms among nurses were evaluated by the Pittsburgh Sleep Quality Index (PSQI) and Hospital Anxiety and Depressive Disorders Rating Scale (HADS), respectively. Results: PSQI and HADS scores were both significantly higher in the nurses working night shifts (P