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Your search keyword '"Nikalje, Ganesh Chandrakant"' showing total 11 results
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11 results on '"Nikalje, Ganesh Chandrakant"'

9. Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in Monoraphidium sp. for Pharmacological and Nutraceutical Applications

Author

Yadav, Kushi, Kumar, Shashi, Nikalje, Ganesh Chandrakant, and Rai, Monika Prakash

Subjects
plant_sciences
Abstract

Carotenoids are essential pigments that play a vital role in the photosynthetic process of algae and are important for human health and nutrition. Despite their potential value, algal carotenoids are being neglected by biofuel industries. Recent research is aimed at producing microalgal lipids and carotenoids simultaneously under abiotic stresses. In this study, the effect of different abiotic stress factors such as Nitrogen (N), Potassium (K), Phosphorous (P), Sulphur (S), light intensity, and photoperiod on biomass, lipid, and carotenoids synthesis in Monoraphidium sp. (MP) were explored utilizing a statistical method. In addition, 2, 7-dichlorofluorescein diacetate (DCF-DA) mediated spectrofluorimetry was employed to detect the ele-vation in ROS level. The results revealed that variations in nutrients and light con-ditions are the key factors in the up-regulation of biomolecules lipids and carote-noids in the algae. The carotenoids profiling was done by Ultra Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectroscopy (UPLC-Q-ToF-MS). Among consisting carotenoids violaxanthin, astaxanthin, and beta-carotene were enhanced by 1.3 folds, 1.19 folds, 1.08 folds, while lutein was declined by 1.32 folds under stressed condition compared to control. The identified carotenoids are rec-ognized for their importance in the pharmaceutical and nutraceutical industries, highlighting the significant role of MP in these sectors.

Published
2023

10. Screening of the Most Effective Media for Bioprospecting Three Indigenous Freshwater Microalgae Species.

Author

Yadav, Kushi, Nikalje, Ganesh Chandrakant, Pramanik, Dibyajyoti, Suprasanna, Penna, and Rai, Monika Prakash

Subjects
*CHLORELLA sorokiniana, *MICROALGAE, *ASTAXANTHIN, *CHLORELLA vulgaris, *CAROTENOIDS, *BIOPROSPECTING, *SCENEDESMUS obliquus, *FRESH water, *SPECIES
Abstract

Microalgae are a natural source of lipids, carotenoids, and other value-added compounds. The combination of nutrients and their precise ratio have a significant impact on the productivity of microalgae-mediated compounds. The biomass, lipid, chlorophyll, and carotenoid production of three microalgae species, namely, Monoraphidium sp., Chlorella sorokiniana, and Scenedesmus obliquus, were investigated by employing standard photoautotrophic media, namely, BG-11, BBM, and HS CHU-10. BBM was found as the most effective medium since it obtained higher biomass, lipids, and carotenoids from microalgae. The lipids and carotenoids were identified using chromatographic and microscopy techniques. The findings showed that although the Monoraphidium sp. biomass productivity was the lowest, it emerged as a substantial producer of astaxanthin, whereas the Chlorella sorokiniana culture could grow in a variety of media and produced β-carotene as a major carotenoid. On the other hand, Scenedesmus obliquus was found to be a considerable source of lipids and β-carotene. This study provided a comprehensive understanding of the appropriate medium selection in order to extract an assortment of value-added compounds from freshwater microalgae species. [ABSTRACT FROM AUTHOR]

Published
2023
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