302 results on '"Nikolskaya, P. P."'
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2. Upper Pliocene–Lower Pleistocene Upper Molasse Belorechensk Formation of Western Ciscaucasia in Context of Regional Neotectonics and Paleogeography
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Trikhunkov, Ya. I., Bachmanov, D. M., Tesakov, A. S., Titov, V. V., Lomov, V. S., Sokolov, S. A., Latyshev, A. V., Simakova, A. N., Syromyatnikova, E. V., Çelik, H., Shchelinsky, V. E., Frolov, P. D., Shalaeva, E. A., and Nikolskaya, P. P.
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- 2024
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3. Blood and bone marrow cell disorders in the stages of progressive diabetes in mice
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N. A. Onishchenko, M. Yu. Karganov, I. B. Alchinova, A. B. Cherepov, O. I. Stepanova, A. A. Metelkin, A. O. Nikolskaya, R. A. Klesov, Kh. Kh. Semenov, E. A. Volkova, M. Yu. Shagidulin, and Yu. B. Basok
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diabetes mellitus ,redox processes ,blood cells ,bone marrow cells ,Surgery ,RD1-811 - Abstract
Objective: to examine how the severity of tissue metabolic disorders affects the dynamics of the state of blood cells and bone marrow (BM) cells in patients with progressive diabetes mellitus (DM).Materials and methods. The genetic model of type 2 diabetes (T2DM) in db/db mutant mice (experimental group, n = 30) was used. Healthy mice of the same line – db/+m (n = 10) and line B10 (n = 5) served as control. The dynamics of laboratory and clinical parameters (blood glucose, glycosylated hemoglobin, body weight) and oxidative metabolism indicators in tissues were monitored FOR 6–6.5 months using Lasma-ST device. The state of blood cells (red blood cells, white blood cells, platelets) and BM cells were examined during the same period. Statistical processing of the results was done with preliminary use of the Shapiro–Wilk test; the significance of differences with the control was assessed using the parametric Student’s t test, at p < 0.05.Results. In the development of T2DM, 3 stages of progressive metabolic disorders were identified: I – adaptation stage (1–2 months); II – progressive maladaptation stage (2.5–4.5 months); III – decompensation stage (from 5.0–6.5 months to death). It was found that in T2DM mice, blood content of red blood cells, Hb and leukocytes was reduced already in stages I–III; but in stage II and especially in stage III, there was increased platelet count and percentage of neutrophils, monocytes, eosinophils with a decrease in lymphocytes. A high percentage of live cells is preserved in the BM in stages I, II and early periods of stage III; in late periods of stage III, live cell percentages are frequently found to be low; in all periods of stage III, the total cell content in the BM is clearly reduced.Conclusion. Hematopoietic processes are inhibited in the BM as T2DM progresses. Individual assessment of the state of BM and its cells at the progressive stages of T2DM may be useful for prognostic purposes.
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- 2024
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4. Successful pregnancy in a woman of late reproductive age with type 1 diabetes mellitus after combined pancreas — kidney transplantation
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E. I. Prokopenko, F. F. Burumkulova, I. G. Nikolskaya, T. S. Kovalenko, and V. A. Petrukhin
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combined pancreas-kidney transplantation ,pregnancy ,chronic kidney disease ,diabetes ,immunosuppression ,complications of pregnancy ,Nutritional diseases. Deficiency diseases ,RC620-627 - Abstract
Women of reproductive age with type 1 diabetes mellitus (DM) and chronic kidney disease stage 5 after successful combined pancreas-kidney transplantation (CPKT) can experience spontaneous pregnancies with a favorable outcome. However, those pregnancies are associated with a high risk of complications: arterial hypertension, preeclampsia, premature birth, progression of diabetic retinopathy. During pregnancy, both gestational diabetes and spontaneous hypoglycemia can develop. This article presents a description of the clinical case of a 42-year-old patient with DM type 1, in whom pregnancy occurred 2 years after successful CPKT. Blood pressure was normal, the fundus condition was stable after previous treatment. The patient received tacrolimus and minimal dose of corticosteroids, at 13 weeks acetylsalicylic acid was prescribed to prevent preeclampsia. At 30 weeks nocturnal episodes of hypoglycemia appeared that were treated by additional intake of carbohydrates with an average glycemic index in combination with animal fats and proteins. At 36 gestational weeks caesarean section was performed due to premature rupture of membranes. A healthy girl was born with weight of 2140 g, height of 48 cm, Apgar score 7/8 points. The mother’s function of the kidney and pancreas grafts remained normal. Preconceptional counseling and multidisciplinary pregnancy management are important for patients after CPKT.
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- 2024
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5. Features of the impact of the COVID-19 pandemic on the prevalence of certain respiratory diseases of infections etiology
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I. A. Sorokin, M. V. Nikolskaya, and V. L. Melnikov
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respiratory diseases ,acute respiratory infections ,coronavirus infection ,morbidity rate ,Medicine - Abstract
The aim of the study was to assess the impact of the COVID-19 pandemic on the incidence of respiratory infections of the population of Penza region in different age groups. Material and methods. The analysis of statistical reporting data (Form 12) on the number of diseases in the service area of the medical organization in Penza region was carried out. Information was sampled on respiratory diseases of infectious etiology for the period from 2015 to 2022 and on COVID-19 morbidity in the period from 2020 to 2022 among patients of different age periods. The data is presented as a calculation per 100 thousand population. Results. In 2020, compared to 2019, there was a pronounced increase in the number of respiratory diseases (p = 0.000) in adults; in 2021, the incidence decreased slightly, being higher until 2022 than in the period before the pandemic (p = 0.000). Among children and adults, there was an increase in the incidence of acute respiratory infections in 2022 compared to 2015 (p < 0.001). Peak incidence of croup and epiglottitis among children and adolescents was observed in 2020. The incidence of COVID-19 among adolescents (8339.6) and adults (7678.5) was significantly higher than the incidence among children (5949.9) (p = 0.000). Discussion. Respiratory diseases of infectious etiology have an acute course, so primary morbidity predominates. In adults, the proportion of primary morbidity is decreasing, which may be related to an increase in chronic diseases of the respiratory system. The weak impact of the pandemic on pediatric respiratory diseases incidence is supported by the low pediatric incidence of COVID-19. The increased incidence of COVID-19 among adolescents is alarming; it supports the assumption that child and young adult populations have proven to be major gateways for the spread of COVID-19 to adults. The high incidence of croup and epiglottitis in 2020 among children (p = 0.024) and adolescents could be one of the symptoms of COVID-19 characteristic of younger age. Conclusions. COVID-19 had no effect on the increase in respiratory disease among children, but children contributed to the spread of infection among adults. There is a trend towards an increase in chronic respiratory disease in adults. RBD of infectious etiology is almost impossible to predict, except for acute laryngitis and tracheitis among adults. In adolescents and children against the background of COVID-19 infection, a large number of more severe symptoms may appear.
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- 2024
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6. Epidemiological and clinical features of measles on the example of an outbreak in the Penza region
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J. Yu. Kurmaeva, A. V. Afonin, S. B. Rybalkin, V. L. Melnikov, and M. V. Nikolskaya
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measles ,morbidity ,clinical features ,anti-epidemic measures ,prevention ,Medicine - Abstract
Currently, many countries around the world, including Russia, are experiencing an increase in measles morbidity. One of the reasons for this rise is a decrease in the number of vaccinated persons and violation of vaccination schemes. Aim of the study was to investigate of epidemiologic and clinical features of the disease during the measles outbreak in Penza Oblast in 2023. Material and Methods. A retrospective analysis of 73 medical records of patients during the outbreak of the disease in the village of Srednaya Elyuzan, Penza region, in 2023 was performed. The diagnosis was confirmed by detection of specific marker (IgM) in the blood by ELISA. Results and discussion. There were 67 children and 6 adults among those who fell ill. Outpatient treatment was provided to 36 persons, and 37 children were hospitalized. Among the children who contracted measles, 63 children were not immunized against measles, 59 of them due to parents’ refusal to immunize them. In all patients, measles was characterized by typical clinical manifestations. Moderate forms of the disease were detected in 43 children and 6 adults (67.1 %), mild – in 24 (32.9 %) children. Conclusions. A characteristic feature of this disease was the presence of more pronounced symptoms and a longer catarrhal period in older children. Thanks to timely preventive and anti-epidemiological measures, the outbreak was localized and further spread of the infection was prevented. Given the repeated possibility of outbreaks, it is necessary to improve vaccine prevention.
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- 2024
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7. Polarization states of ZnO-based thin films probed by magnetic circular dichroism spectroscopy
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Samoshkina, Yu., Petrov, D., Nikolskaya, A., and Dudin, Yu.
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- 2024
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8. Functional efficiency of pancreatic cell-engineered construct in an animal experimental model for type I diabetes
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N. V. Baranova, A. S. Ponomareva, L. A. Kirsanova, A. O. Nikolskaya, G. N. Bubentsova, Yu. B. Basok, and V. I. Sevastianov
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type i diabetes mellitus ,islets of langerhans ,cell-engineered construct ,pancreas ,decellularization, tissue-specific scaffold ,Surgery ,RD1-811 - Abstract
The creation of a cell-engineered pancreatic construct (CEPC) from islets of Langerhans and biocompatible matrix carrier (framework/scaffold), which imitates the native microenvironment of pancreatic tissue, is an approach to the treatment of type I diabetes mellitus (T1D).The objective of this work is to conduct a comparative analysis of the functional efficacy of CEPC and isolated rat islets of Langerhans after intraperitoneal administration into rats with experimental T1D.Materials and method. T1D was induced in rats by injecting low-dose (15 mg/ kg) streptozotocin (STZ) for 5 days. CEPC samples were created using viable and functional allogeneic isolated islets of Langerhans and tissue-specific scaffold obtained by decellularization of human pancreatic fragments. The rats received intraperitoneal injection of allogeneic islets of Langerhans (experimental group 1, n = 4) and CEPC (experimental group 2, n = 4). Control group rats received no treatment (n = 4). Blood glucose levels in the rats were measured, and the pancreas and kidneys of the experimental animals were examined histologically. The follow-up period for all animals continued for 10 weeks. Results. In experimental group 1, on day 7 after injection of Langerhans islets, glycemia decreased significantly from 28.2 ± 4.2 mmol/L to 13.4 ± 2.6 mmol/L. This fall persisted for 7 weeks, following which blood sugar increased to nearly their initial levels (prior to islets administration). In experimental group 2, on day 7 after CEPC administration, there was a more noticeable drop in blood sugar levels from 25.8 ± 5.1 mmol/L to 6.3 ± 2.7 mmol/L compared to experimental group 1. By the 10th week of the experiment, the average glucose level was two times lower than it was at the beginning. Blood glucose levels dropped more sharply in the CEPC group than in the islet group (by 75.6% and 52.5%, respectively).Conclusion. In T1D rats, CEPC has a more potent antidiabetic effect than islets of Langerhans. Thus, it has been shown that a tissue-specific scaffold may be used to create bioartificial pancreas in order to increase the functional efficiency of islets.
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- 2024
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9. Pregnancy after kidney transplantation: clinical features, complications and outcomes
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E. I. Prokopenko, I. G. Nikolskaya, A. V. Vatazin, F. F. Burumkulova, and D. V. Gubina
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kidney transplantation ,immunosuppression ,pregnancy, complications ,pregnancy outcomes ,renal graft survival ,Surgery ,RD1-811 - Abstract
Pregnancy after kidney transplantation (KT) has become more common, but the risk of complications and adverse obstetric outcomes in this group of women remains high.Objective: to study pregnancy complications and outcomes in kidney recipients and renal graft (RG) survival after childbirth.Material and methods. The study included 22 pregnancies in 20 women with RG (transplants performed in 2006–2020). The comparison group consisted of 20 healthy women who had 20 pregnancies. Frequency and nature of pregnancy complications, neonatal health indicators, and pregnancy outcomes were evaluated. Graft survival was compared in the main group and in a group of 102 women after KT who did not have pregnancies.Results. Compared with healthy women, RG recipients had a higher rate of preeclampsia (25% and 0%, p = 0.047), fetal growth restriction (30% and 0%, p = 0.020), gestational diabetes (40% and 5%, p = 0.020), asymptomatic bacteriuria (35% and 5%, p = 0.044), preterm birth (60% and 0%, p < 0.001), and cesarean section (70% and 10%, p < 0.001). Median gestational age and birth weight were significantly lower in women with RG: 36.0 [33.9; 37.4] vs. 38.9 [38.9; 39.6] weeks, p < 0.001, and 2405 [2023; 2958] vs. 3355 [3200; 3690] g, p < 0.001, respectively. The rate of favorable pregnancy outcomes after KT was 81.8%, or 90% when early pregnancy loss is excluded. Two children were found to have genetic diseases passed from the mother. Graft survival did not differ between RG recipients with and without pregnancy, p = 0.272.Conclusions. Pregnancy outcomes in patients with RG are generally favorable, pregnancy and childbirth do not affect graft survival. When planning pregnancy after KT, it is necessary to consider the risk of complications and the possibility of transmitting genetic disorders to offspring.
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- 2024
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10. Biomimetic approach to the design of artificial small‑diameter blood vessels
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E. A. Nemets, Yu. V. Belov, K. S. Kiryakov, N. V. Grudinin, V. K. Bogdanov, K. S. Filippov, A. O. Nikolskaya, I. Yu. Tyunyaeva, A. A. Vypryshko, V. M. Zaxarevich, Yu. B. Basok, and V. I. Sevastianov
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vascular prosthesis ,electrospinning ,tubular porous scaffold ,polycaprolactone ,gelatin ,heparin ,platelet lysate ,endothelial cells ,cytotoxicity ,implantation ,rat aorta ,Surgery ,RD1-811 - Abstract
Objective: To create 2-mm diameter multilayer porous tubular scaffolds (PTS) with characteristics that resemble small-diameter native blood vessels in terms of characteristics.Materials and methods. PTS made of polycaprolactone (PCL, MM 80000) with a PCL-made sealing coat/layer with gelatin addition (PCL-gelatin) with a diameter of 2 mm were created by electrospinning (NANON-01A). Bioactive coating was applied to the PTS surface by sequential incubation in solutions of bovine serum albumin, heparin (Hp), and platelet lysate (PL). Cytotoxicity was investigated under conditions of direct contact of PTS with a monolayer of NIH/3T3 mouse fibroblasts. Viability of human umbilical vein endothelial cells (EA.hy926) was evaluated using Live/Dead® Viability/Cytotoxicity Kit. Permeability and blood flow parameters of the PTS implanted in the infrarenal section of the rat aorta were recorded using Doppler imaging.Results. A three-layer PTS construct with an inner diameter of 2 mm was developed. Its inner and outer layers were formed from 0.2 mL of PCL solution, and the middle sealing coat/layer was from 0.5 mL of PCL with addition of 30% (by weight of polymer) gelatin. Introduction of the sealing coat/layer reduced surgical porosity (SP) from 56.2 ± 8.7 mL/(cm2·min) for a single-layer PTS made of pure PCL to 8.9 ± 2.6 mL/(cm2·min) for a three-layer PTS. The resulting PTS demonstrated physicomechanical characteristics similar to those of native blood vessels; it also showed no cytotoxicity. Application of a bioactive coating of Hp and PL allowed for increased in vitro adhesion and proliferation of endothelial cells. The technique of implantation of 10 mm long fragments of three-layer PTS into the infrarenal section of a rat aorta was corrected, thus minimizing blood loss and narrowing the anastomosis site. In an acute experiment, it was proven that the prostheses were patent and that blood flow parameters (systolic and diastolic velocity, resistivity index) were close to the corresponding indicators of native rat aorta.Conclusion. The developed three-layer PTS constructs have low SP and physicomechanical properties close to those of native blood vessels. Bioactive coating improves the in vitro matrix properties of PTS relative to human endothelial cells. At short-term implantation into the aorta of experimental animals, PTS showed no early thrombosis, while blood flow parameters were close to those of native rat aorta. Thus, three-layer PTS with bioactive coating can be used as a scaffold for creation of in situ tissue-engineered construct of a small-diameter blood vessel.
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- 2024
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11. ‘Congolité’: Dividing Lines in the Political Life of the Contemporary DRC
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M. V. Nikolskaya
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identity ,congolité ,democratic republic of the congo ,citizenship ,banyamulenge ,language in politics ,elections ,International relations ,JZ2-6530 - Abstract
The article is devoted to the study of political identity in the Democratic Republic of Congo. The author pays particular attention to the modern interpretation of the concept of ‘Congolité’ – Congolese identity, which is in many ways identical to the idea of national citizenship. The concept of ‘Congolité’ itself is considered in the article as a set of legal norms, as an instrument of electoral struggle and as a symbolic construct. The concept has acquired a new meaning in the context of the 2021 draft law, and its return to domestic politics is significant for understanding the mood of Congolese society. The author also tries to identify the main lines of the identity debate and concludes that one of its constants is the problem of the political-legal status of the Rwandafon minority (Banyarwanda / Banyamulenge), and another is regional-linguistic fragmentation, which is reinforced by economic-demographic specificity. Both of these factors have a significant impact on internal voting patterns. The discourse of autochthonous ‘purity’ is seen as exacerbating this fragmentation and as generating conflict in Congo’s relations with states in the region, particularly Rwanda.
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- 2024
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12. Genetic Markers of Helicobacter pylori Resistance to Clarithromycin and Levofloxacin in Moscow, Russia
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Natalia Bodunova, Larisa Tsapkova, Vera Polyakova, Irina Baratova, Konstantin Rumyantsev, Natalia Dekhnich, Karina Nikolskaya, Margarita Chebotareva, Irina Voynovan, Elena Parfenchikova, Galina Pronina, Ekaterina Chernikova, and Dmitry Bordin
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Helicobacter pylori ,antibiotic resistance ,methods for determining H. pylori resistance ,molecular genetic diagnostics ,phenotypic methods for determining antibiotic resistance ,geographical distribution of H. pylori antibiotic resistance ,Biology (General) ,QH301-705.5 - Abstract
The Maastricht VI/Florence consensus recommends, as one of the measures to enhance the efficacy of Helicobacter pylori infection eradication, a personalized treatment approach involving the selection of an antimicrobial agent based on the pre-determined resistance of H. pylori. To address the need to develop test systems for personalized drug selection, this study was designed to analyze the molecular resistance of H. pylori using a newly developed Sanger sequencing test platform. The characteristics of the test system were determined on 25 pure culture samples of H. pylori with known resistance. Sensitivity and specificity for detecting resistance to clarithromycin was 100% and those to levofloxacin were 93% and 92%, respectively. The test system has been tested in real clinical practice on 112 H. pylori-positive patients who had not previously received proton pump inhibitors (PPIs) or antibacterial drugs. Mutations indicating resistance to clarithromycin were found in 27 (24%) samples and those indicating resistance to levofloxacin were found in 26 (23%) samples. Double resistance was observed in 16 (14%) samples. The most common mutations leading to clarithromycin resistance were 2143G and 2142G and to levofloxacin resistance—261A and 271A in the gyrA gene, which account for 69% of all identified genetic determinants in levofloxacin-resistant bacteria. Thus, a personalized approach to the selection of H. pylori eradication therapy based on the detection of bacterial resistance before prescribing first-line therapy could help to avoid the prescription of ineffective H. pylori eradication therapies and, overall, contribute to the control of antibiotic resistance of H. pylori.
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- 2024
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13. Deep learning for track recognition in pixel and strip-based particle detectors
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Bakina, O., Baranov, D., Denisenko, I., Goncharov, P., Nechaevskiy, A., Nefedov, Yu., Nikolskaya, A., Ososkov, G., Rusov, D., Shchavelev, E., Sun, S. S., Wang, L. L., Zhang, Y., and Zhemchugov, A.
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High Energy Physics - Experiment ,Physics - Instrumentation and Detectors - Abstract
The reconstruction of charged particle trajectories in tracking detectors is a key problem in the analysis of experimental data for high-energy and nuclear physics. The amount of data in modern experiments is so large that classical tracking methods, such as the Kalman filter, cannot process them fast enough. To solve this problem, we developed two neural network algorithms based on deep learning architectures for track recognition in pixel and strip-based particle detectors. These are TrackNETv3 for local (track by track) and RDGraphNet for global (all tracks in an event) tracking. These algorithms were tested using the GEM tracker of the BM@N experiment at JINR (Dubna) and the cylindrical GEM inner tracker of the BESIII experiment at IHEP CAS (Beijing). The RDGraphNet model, based on a reverse directed graph, showed encouraging results: 95% recall and 74% precision for track finding. The TrackNETv3 model demonstrated a recall value of 95% and 76% precision. This result can be improved after further model optimization.
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- 2022
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14. Cladribine chemotherapy of multifocal, multisystemic form of Rosai–Dorfman disease: literature review and case report
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V. G. Potapenko, D. S. Abramov, V. V. Baykov, T. L. Grigorieva, M. S. Selinkina, L. O. Nikolskaya, and J.-F. Emile
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rosai–dorfman disease ,cladribine ,histiocytosis ,emperiopolesis ,map2k1 ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Rosai–Dorfman disease is the most frequent variant of non-Langerhans cell histiocytosis. Local forms can be resected or irradiated. If the process involves multiple organs, systemic chemotherapy can cure some patients. This article includes literature review and a case report of a 34-year-old patient with multifocal, multisystemic form of Rosai–Dorfman disease with bone and pleural involvement. The diagnosis was based on histological, immunohistochemical, and molecular studies of tumor tissue. Since November 2021, 6 courses of chemotherapy with cladribine and 8 infusions of zolendronic acid were carried out with achievement of durable remission. The tolerance was acceptable.
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- 2024
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15. FEATURES OF THE PLACEMENT OF DUMPS DURING THE DEVELOPMENT OF UPLAND DEPOSITS: QUALITY MANAGEMENT IN INDUSTRIAL AND MANUFACTURING ENGINEERING
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Zulfiia A. Asilova and Olga V. Nikolskaya
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dump ,slope angle ,base strength ,overburden ,tensile strength ,bearing capacity ,quality of the development of mineral resource deposits ,quality management in industrial and manufacturing engineering ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
The purpose of this paper was to determine the specifics of the placement of dumps during the development of upland deposits and to develop recommendations to improve quality management in industrial and manufacturing engineering during the development of mineral resource deposits. As a result of the performed research, it was proved that the placement of dumps during the development of upland deposits requires more calculations for planning and ensuring stability than for dumps located on a horizontal surface. Assessment of the stability of dumps on the slope is a key component of ensuring the safety of dumps. One of the main factors affecting the stability of dumps on an inclined base is the bearing capacity of the base. When planning the area for the dump, the bearing capacity and strength of the base rocks are calculated. The article describes a method for determining the loading of the dump depending on the bearing capacity of the base of the dump of overburden rocks. The features of the placement of overburden dumps during the development of upland deposits are revealed, taking into account the planning of the base for the dump, depending on the strength characteristics of the base and the slope angle. The theoretical significance of the results obtained is due to their rethinking of the essence of the development of mineral resource deposits from the position of quality management in industrial and manufacturing engineering. The practical significance of the authors’ developments is that the authors’ methodology of determining the load of the dump depending on the carrying capacity of the base dump of overburden rocks will allow improving quality management in industrial and manufacturing engineering of the development of mineral resource deposits.
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- 2024
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16. COMPARATIVE ANALYSIS OF INDICATORS OF ECONOMIC EF-FICIENCY OF CULTIVATION OF CHERRY SEEDLINGS WITH DRIP IRRIGATION
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Olga A. Nikolskaya, Elena V. Seminchenko, and Andrey V. Solonkin
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irrigation ,seedlings ,variety ,profitability ,economic efficiency ,Agriculture ,Science - Abstract
Background. The increase in fruit production to a deficit-free volume of consumption by the population and the needs of industrial processing is due to the need to expand the area of garden plantings. An integral part of the organization of the laying of garden plantings is the nursery industry that grows planting material. A lot depends on the quality of the planting material. The choice of a seedling affects the further growth, development, fruiting, yield and immunity of the future fruit tree. In the conditions of a nursery of stone crops of open ground, it is important to determine the irrigation regime, which, in combination with the use of fertilizers, will provide the highest yield of seedlings while saving all types of resources. Purpose. To determine the economic efficiency indicators of the recommended irrigation technology in combination with mineral fertilizing of cherry seedlings. The assessment of economic efficiency in our experiments was carried out by calculating the profit received, taking into account all costs in the nursery and the level of profitability. Materials and methods. The object of research was annual cherry seedlings of the Vasilisa variety. The scheme of two-factor experience provided for the study of three options for each factor. Factor A is the water regime of the soil and factor B is mineral fertilizing. Results. It has been proved that when growing seedlings of stone crops on light chestnut soils, the effectiveness of fertilizer application in combination with a differentiated irrigation method is high, in which the depth of soaking and, accordingly, the irrigation rate changes. Conclusion. The analysis of the data on the economic efficiency of growing seedlings showed that at the selling price of seedlings 170 (grade 1) and 120 (grade 2) rubles per seedling, when growing them against the background of the A3 water regime in combination with top dressing with Izabion and Master, the profitability was the highest and amounted to 155 and 154%. At the same time, the profitability of the control variant was 99%.
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- 2024
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17. Design of SAHA-Loaded PLGA Nanoparticles Aimed for Use in Breast Cancer Combination Therapy
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Gulyaev, I. A., Sokol, M. B., Klimenko, M. A., Mollaeva, M. R., Yabbarov, N. G., Chirkina, M. V., and Nikolskaya, E. D.
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- 2023
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18. Mineralization of Shells of Emulsion Polyelectrolyte Microcapsules by Calcium Carbonate
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Buslenko, A. V., Bukreeva, T. V., Chistyakov, A. P., Vantsian, M. A., Trushina, D. B., Nikolskaya, E. D., Mollaeva, M. R., Yabbarov, N. G., and Sokol, M. B.
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- 2023
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19. The Effect of the Positive Electrode Properties on the Activation Time of the Lead–Perchloric Acid–Lead Dioxide-Based Reserve Chemical Power Sources
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Shcheglov, P. A., Samsonov, D. A., Pavlenkov, A. B., Kulova, T. L., Rychagov, A. Yu., Nikolskaya, N. F., Shiryaev, A. A., and Skundin, A. M.
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- 2023
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20. A Tissue-Engineered Construct Based on a Decellularized Scaffold and the Islets of Langerhans: A Streptozotocin-Induced Diabetic Model
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Victor I. Sevastianov, Anna S. Ponomareva, Natalia V. Baranova, Aleksandra D. Belova, Lyudmila A. Kirsanova, Alla O. Nikolskaya, Eugenia G. Kuznetsova, Elizaveta O. Chuykova, Nikolay N. Skaletskiy, Galina N. Skaletskaya, Evgeniy A. Nemets, Yulia B. Basok, and Sergey V. Gautier
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decellularized scaffold ,streptozotocin-induced diabetes mellitus ,islets of Langerhans ,islet transplantation ,Science - Abstract
Producing a tissue-engineered pancreas based on a tissue-specific scaffold from a decellularized pancreas, imitating the natural pancreatic tissue microenvironment and the islets of Langerhans, is one of the approaches to treating patients with type 1 diabetes mellitus (T1DM). The aim of this work was to investigate the ability of a fine-dispersed tissue-specific scaffold (DP scaffold) from decellularized human pancreas fragments to support the islets’ survival and insulin-producing function when injected in a streptozotocin-induced diabetic rat model. The developed decellularization protocol allows us to obtain a scaffold with a low DNA content (33 [26; 38] ng/mg of tissue, p < 0.05) and with the preservation of GAGs (0.92 [0.84; 1.16] µg/mg, p < 0.05) and fibrillar collagen (273.7 [241.2; 303.0] µg/mg, p < 0.05). Rat islets of Langerhans were seeded in the obtained scaffolds. The rats with stable T1DM were treated by intraperitoneal injections of rat islets alone and islets seeded on the DP scaffold. The blood glucose level was determined for 10 weeks with a histological examination of experimental animals’ pancreas. A more pronounced decrease in the recipient rats’ glycemia was detected after comparing the islets seeded on the DP scaffold with the control injection (by 71.4% and 51.2%, respectively). It has been shown that the DP scaffold facilitates a longer survival and the efficient function of pancreatic islets in vivo and can be used to engineer a pancreas.
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- 2024
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21. Offline Software and Computing for the SPD experiment
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Andreev, V., Belova, A., Galoyan, A., Gerassimov, S., Golovanov, G., Goncharov, P., Gribowsky, A., Maletic, D., Maltsev, A., Nikolskaya, A., Oleynik, D., Ososkov, G., Petrosyan, A., Rezvaya, E., Shchavelev, E., Tkachenko, A., Uzhinsky, V., Verkheev, A., and Zhemchugov, A.
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Physics - Instrumentation and Detectors - Abstract
The SPD (Spin Physics Detector) is a planned spin physics experiment in the second interaction point of the NICA collider that is under construction at JINR. The main goal of the experiment is the test of basic of the QCD via the study of the polarized structure of the nucleon and spin-related phenomena in the collision of longitudinally and transversely polarized protons and deuterons at the center-of-mass energy up to 27 GeV and luminosity up to $10^{32}$ 1/(cm$^2$ s). The data rate at the maximum design luminosity is expected to reach 0.2 Tbit/s. Current approaches to SPD computing and offline software will be presented. The plan of the computing and software R&D in the scope of the SPD TDR preparation will be discussed., Comment: arXiv admin note: substantial text overlap with arXiv:2102.00442
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- 2021
22. Ariadne: PyTorch Library for Particle Track Reconstruction Using Deep Learning
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Goncharov, Pavel, Schavelev, Egor, Nikolskaya, Anastasia, and Ososkov, Gennady
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Physics - Data Analysis, Statistics and Probability ,High Energy Physics - Experiment ,6804, 68M10, 97R40 ,H.3 ,F.3 ,B.4 - Abstract
Particle tracking is a fundamental part of the event analysis in high energy and nuclear physics. Events multiplicity increases each year along with the drastic growth of the experimental data which modern HENP detectors produce, so the classical tracking algorithms such as the well-known Kalman filter cannot satisfy speed and scaling requirements. At the same time, breakthroughs in the study of deep learning open an opportunity for the application of high-performance deep neural networks for solving tracking problems in a dense environment of experiments with heavy ions. However, there are no well-documented software libraries for deep learning track reconstruction yet. We introduce Ariadne, the first open-source library for particle tracking based on the PyTorch deep learning framework. The goal of our library is to provide a simple interface that allows one to prepare train and test datasets and to train and evaluate one of the deep tracking models implemented in the library on the data from your specific experiment. The user experience is greatly facilitated because of the system of gin-configurations. The modular structure of the library and abstract classes let the user develop his data processing pipeline and deep tracking model easily. The proposed library is open-source to facilitate academic research in the field of particle tracking based on deep learning., Comment: 5pages, 1 figures, The XXIV International Scientific Conference of Young Scientists and Specialists (AYSS-2020)
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- 2021
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23. Political Dimension of Swahili in East Africa: Country and Regional Aspects
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M. V. Nikolskaya
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language policy ,east africa ,identity ,nation building ,integration ,kiswahili ,Political science (General) ,JA1-92 - Abstract
Kiswahili is an East African Bantu language with around 150 million speakers, including those for whom it is a mother tongue or a language of confident proficiency. Its historical geographic domain spans Tanzania, Kenya and Uganda. All three were part of British East Africa from the early 1920s up until decolonization. At present, their shared history, culture, geographical proximity and integration aspirations make them the core countries in the region. It would be logical therefore to assume that the position of Kiswahili should be growing stronger, yet in reality its status, functions and spread differ across all the three countries. This article seeks to identify the reasons for this phenomenon and analyze whether Kiswahili has the right potential to become a tool for regional political communication and integration.
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- 2024
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24. Evaluation of the Synergistic Effect of the Combination of Doxorubicin and Vorinostat on Breast Tumor Cells Using the Loewe Model
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М. R. Mollaeva, N. G. Yabbarov, М. В. Sokol, М. V. Chirkina, and E. D. Nikolskaya
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doxorubicin ,vorinostat ,cytotoxic activity ,synergy ,apoptosis ,breast cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
The combination of two or more medications is increasingly more common in the development of new treatment guidelines for common diseases. Thus, the use of combinations of histone deacetylase inhibitors with chemotherapeutic agents is a current trend in solid tumor treatment. The aim of this study was to investigate an effective vorinostat (SAHA) to doxorubicin (DOX) ratio for the treatment of different subtypes of breast cancer. The survival of HCC-1954, SKBR-3, MCF-7, MCF-7/ADR, MDA-MB-231 cell lines was assessed under incubation conditions with 64 variants of SAHA and DOX combinations using the MTT assay. This made it possible to determine the effect of interactions of SAHA/DOX combinations (antagonistic, additive, synergistic), as well as calculate the SAHA/DOX synergy index using the Loewe additivity model. The effect of SAHA/DOX ratios with the highest synergistic index for each tumor cell line was confirmed using the Chou-Talalay method. It was shown that the SAHA/DOX combination exhibited the greatest synergism in relation to HCC-1954, MCF-7/ADR and SKBR-3 cell lines belonging to the HER2-positive subtype. The average value of SAHA/DOX ratio with the highest synergy against breast cancer cells was 30:1 (SAHA to DOX, respectively). The ability of SAHA/DOX combination to effectively trigger apoptosis was confirmed in the most sensitive to SAHA/DOX therapy HCC-1954 cells. Thus, the Loewe model made it possible to identify the drug combination with the highest synergistic anticancer effect, which was confirmed using Chou-Talalay method. The data obtained demonstrates great potential of SAHA/DOX combination (30:1) for the treatment of HER2-positive breast cancer.
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- 2023
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25. The Warsaw Autumn International Music Festival — Overcoming the Boundaries between East and West
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Nikolskaya Irina I.
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warsaw autumn ,festival ,contemporary classical music ,avant-garde ,dodecaphony ,sonoristics ,neoclassicism ,aleatoric music ,experimental music ,Arts in general ,NX1-820 - Abstract
In the late 1940s — early 1950s, a huge gap appeared between the development of musical cultures of Western and Eastern Europe, and it was Poland that initiated bridging it. Therefore, the emergence of the Warsaw Autumn Festival of contemporary music, which has become the largest, was not coincidental. The article is devoted to studying this cultural phenomenon, which has a significant scientific novelty, because the Warsaw Autumn has not yet been subjected to a detailed scientific analysis in Russian musicology. According to the organizers of the new festival — young Polish composers Tadeusz Baird and Kazimierz Serocki, — Warsaw was to become a center of contemporary music, no less important than the avant-garde music festivals in Darmstadt, Donaueschingen, Cologne or Milan. However, the purpose of the Warsaw Autumn is more ambitious — to provide a complete aesthetic and stylistic picture of modern music, and not just avant-garde music, as was the case in Western European countries. The repertoire policy of the festival covered several areas: avantgarde music, more traditional music, the classics of the 20th century, and promotion of Polish music. The festival laid claim to being a reliable display of contemporary music in the world, reacted to the changes in global music, and soon became the largest music arena of the 20th century. It was attended by the most prominent composers of the East and West. For the socialist countries, it became a true “window on Europe” and a platform for mastering new techniques of composition. Without the Warsaw Autumn festival and its profound influence on composers of socialist countries, it would be utterly impossible to imagine the development of musical art of the entire region. This article suggests focusing on the early period of the festival, from 1956 (when it was established) to the early 1980s.
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- 2023
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26. Photoluminescence of ion-synthesized 9R-Si inclusions in SiO2/Si structure: effect of irradiation dose and oxide film thickness
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Nikolskaya, Alena, Belov, Alexey, Mikhaylov, Alexey, Konakov, Anton, Tetelbaum, David, and Korolev, Dmitry
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Condensed Matter - Materials Science - Abstract
The study of hexagonal silicon polytypes attracts special attention due to their unique physical properties compared to the traditional cubic phase of Si. Thus, for some hexagonal phases, a significant improvement in the emission properties has been demonstrated. In this work, the luminescent properties of SiO2/Si structures irradiated with Kr+ ions at different doses and annealed at 800 {\deg}C have been systematically investigated. For such structures, a photoluminescence line at ~ 1240 nm is observed and associated with the formation of hexagonal 9R-Si phase inclusions. It is found that the variation in the thickness of oxide film and the relative position of ion distribution profile and film / substrate interface leads to a regular change in the luminescence intensity. The nature of the observed dependencies is discussed as related to the interplay between the desirable 3C-Si -> 9R-Si structural transition and generation of nonradiative defects. The revealed regularities suggest optimal ion irradiation conditions for synthesis of optically active 9R-Si phase in diamond-like silicon.
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- 2021
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27. Ion-Beam Modification of Metastable Gallium Oxide Polymorphs
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Tetelbaum, D. I., Nikolskaya, A. A., Korolev, D. S., Belov, A. I., Trushin, V. N., Dudin, Yu. A., Mikhaylov, A. N., Pechnikov, A. I., Scheglov, M. P., Nikolaev, V. I., and Gogova, D.
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Condensed Matter - Materials Science ,Physics - Applied Physics - Abstract
Gallium oxide with a corundum structure ({\alpha}-Ga2O3) has recently attracted great attention in view of electronic and photonic applications due to its unique properties including a wide band gap exceeding that of the most stable beta phase (\b{eta}-Ga2O3). However, the lower thermal stability of the {\alpha}-phase at ambient conditions in comparison with the \b{eta}-phase requires careful investigation of its resistance to other external influences such as ion irradiation, ion doping, etc. In this work, the structural changes under the action of Al+ ion irradiation have been investigated for a polymorphic gallium oxide layers grown by hydride vapor phase epitaxy on c-plane sapphire and consisting predominantly of {\alpha}-phase with inclusions of {\alpha}(\k{appa})-phase. It is established by the X-ray diffraction technique that inclusions of {\alpha}(\k{appa})-phase in the irradiated layer undergo the expansion along the normal to the substrate surface, while there is no a noticeable deformation for the {\alpha}-phase. This speaks in favor of the different radiation tolerance of various Ga2O3 polymorphs, especially the higher radiation tolerance of the {\alpha}-phase. This fact should be taken into account when utilizing ion implantation to modify gallium oxide properties in terms of development of efficient doping strategies.
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- 2021
28. Ion implantation in \b{eta}-Ga2O3: physics and technology
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Nikolskaya, Alena, Okulich, Evgenia, Korolev, Dmitry, Stepanov, Anton, Nikolichev, Dmitry, Mikhaylov, Alexey, Tetelbaum, David, Almaev, Aleksei, Bolzan, Charles Airton, Buaczik, Antônio Jr, Giulian, Raquel, Grande, Pedro Luis, Kumar, Ashok, Kumar, Mahesh, and Gogova, Daniela
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Condensed Matter - Materials Science - Abstract
Gallium oxide and in particular its thermodynamically stable \b{eta}-Ga2O3 phase is within the most exciting materials in research and technology nowadays due to its unique properties, such as an ultra-wide band gap and a very high breakdown electric field, finding a number of applications in electronics and optoelectronics. Ion implantation is a traditional technological method used in these fields, and its well-known advantages can contribute greatly to the rapid development of physics and technology of Ga2O3-based materials and devices. Here, the current status of ion beam implantation in \b{eta}-Ga2O3 is reviewed. The main attention is paid to the results of experimental study of damage under ion irradiation and the properties of Ga2O3 layers doped by ion implantation. The results of ab initio theoretical calculations of the impurities and defects parameters are briefly presented, and the physical principles of a number of analytical methods used to study implanted gallium oxide layers are highlighted. The use of ion implantation in the development of such Ga2O3-based devices as metal oxide field effect transistors, Schottky barrier diodes, and solar-blind UV detectors, is described together with systematical analysis of the achieved values of their characteristics. Finally, the most important challenges to be overcome in this field of science and technology are discussed.
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- 2021
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29. A device for exposure to a permanent magnetic field and a system multifactorial analysis in a magnetobiological experiment
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Igor L. Slobodyanyuk, Yulia V. Nikolskaya, Sergey E. Kislyaev, Polina G. Pichugina, Tatyana A. Antipova, Lyubov V. Matveeva, Vyacheslav A. Machikhin, Viktor A. Polyakov, Eric S. Gasparov, and Andrey G. Mayorov
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effects of magnetic fields ,multifactorial analysis ,magnetobiological experiment ,Physics ,QC1-999 ,Electronics ,TK7800-8360 - Abstract
Background. Interest in the biological effects of electromagnetic radiation on biological objects is associated with the possibility of their use in solving a number of fundamental and applied problems: from the consideration of biological feedbacks in real time, which allows the development of electronic medical complexes, to the creation of neuro-computer interfaces, including components of living cells. Aim. The paper shows the development of a device for influencing biological objects with a permanent magnetic field, which allows modeling the basic parameters of the physical factor, for conducting a magnetobiological experiment with normalization of the effects of non-ionizing radiation of the specified parameters. Methods. The device for influencing biological objects of constant magnetic field allows to obtain, in a large volume, the most homogeneous magnetic field. Results. Mathematical modeling of changes in the morphofunctional state of the adrenal cortex according to eleven biological parameters at different periods of exposure to normalized constant magnetic field when exposed to the maximum permissible level was evaluated by the deviation of the interval indicator and its normalized value. The effect of this factor led to a deviation of the integral index in the glomerular zone of the adrenal glands on the 30th day was – 6,53 ± 1,30*10-2, later on the 60th day the deviation increased by 8,28 ± 1,55*10-2, on the 90th 4,84 ± 1,50*10-2, then on the 120th day, it did not differ from normalized. Conclusion. After the termination of the constant magnetic field in a series of 15–30 mT experiments, the morphofunctional state of the thymus returned to normal. Thus, the device is recommended for use in experimental activities.
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- 2023
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30. Solving Black-Box Optimization Challenge via Learning Search Space Partition for Local Bayesian Optimization
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Sazanovich, Mikita, Nikolskaya, Anastasiya, Belousov, Yury, and Shpilman, Aleksei
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Computer Science - Machine Learning - Abstract
Black-box optimization is one of the vital tasks in machine learning, since it approximates real-world conditions, in that we do not always know all the properties of a given system, up to knowing almost nothing but the results. This paper describes our approach to solving the black-box optimization challenge at NeurIPS 2020 through learning search space partition for local Bayesian optimization. We describe the task of the challenge as well as our algorithm for low budget optimization that we named \texttt{SPBOpt}. We optimize the hyper-parameters of our algorithm for the competition finals using multi-task Bayesian optimization on results from the first two evaluation settings. Our approach has ranked third in the competition finals., Comment: Accepted to Proceedings of Machine Learning Research: NeurIPS 2020 Competition and Demonstration Track
- Published
- 2020
31. Comparative analysis of the pharmacokinetic parameters of transdermal and injectable forms of nicotinamide
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V. A. Ryzhikova, E. G. Kuznetsova, O. M. Kuryleva, L. A. Salomatina, S. V. Kursakov, A. O. Nikolskaya, and V. I. Sevastianov
- Subjects
transdermal therapeutic system ,transdermal patch ,antioxidant ,nicotinamide ,pharmacokinetics ,Surgery ,RD1-811 - Abstract
In recent years, oxidative stress, characterized by excess free radicals in the body, has been called the cause of many diseases. There is an active search for drugs with antioxidant properties that are suitable for long-term maintenance therapy. Nicotinamide (NAM), an antioxidant, is used to treat a variety of diseases, usually in oral or injectable form. Given the peculiarities of the drug regimen (dose, prolonged administration), a new dosage form of NAM, a microemulsion-based transdermal patch (TP), containing 20 mg/10 cm2 of NAM, has been proposed.The objective of this work is to compare the pharmacokinetic parameters of intramuscular and transdermal NAM administration in animal experiments for 24 hours.Materials and methods. We used laboratory samples of nicotinamide TP based on a microemulsion-based transdermal delivery emulsion (TDS) with different content of sodium docusate transfer activator. The pharmacokinetics of transdermal and intramuscular injections were studied in male Chinchilla rabbits weighing 3.5–4.0 kg. Plasma NAM levels of the experimental animals were determined by high-performance liquid chromatography using a specially designed method on NUCLEODUR PFP columns (5 μm, 250 × 4.6 mm) using the mobile phase acetonitrile: deionized water. The samples were preliminarily purified by solid-phase extraction using Chromabond C18 Hydra cartridges.Results. When administered intramuscularly, the maximum blood NAM level was 13.3±1 μg/mL; when NAM transdermal forms were applied in the same dosage with different contents of the transfer activator, the levels did not differ significantly – 3.1 and 3.2 μg/mL. It was shown that in transdermal administration of NAM, concentration of the active substance remained at a constant level for ~6 hours. The bioavailability of NAM with transdermal administration was calculated relative to intramuscular administration: 1.43 for TP with 9.8% docusate sodium and 1.84 with 3.3% docusate sodium.Conclusion. NAM has a higher bioavailability when administered transdermally at 20 mg than when administered intramuscularly in the same dose. With transdermal administration, NAM concentration can be maintained at a constant level for a long time, without the jumps that are typical of intramuscular administration.
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- 2023
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32. Intraperitoneal injection of cell-engineered pancreas in rats with experimental type i diabetes (preliminary results)
- Author
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A. S. Ponomareva, N. V. Baranova, A. O. Nikolskaya, L. A. Kirsanova, N. A. Onishchenko, Z. Z. Gonikova, G. N. Bubentsova, Е. A. Volkova, Yu. B. Basok, and V. I. Sevastianov
- Subjects
pancreas ,islets of langerhans ,cellengineered construct ,tissuespecific scaffold ,diabetes model ,Surgery ,RD1-811 - Abstract
Creation of a bioartificial pancreas, including a cell-engineered construct (CEC) formed from pancreatic islets (islets of Langerhans) and a biocompatible matrix mimicking the native microenvironment of pancreatic tissue, is one of the approaches to the treatment of type 1 diabetes mellitus (T1D).Objective: to conduct preliminary in vivo studies of the functional efficacy of intraperitoneal injection of a cell-engineered pancreatic endocrine construct and a suspension of rat pancreatic islets in an experimental T1D model.Materials and methods. Tissue-specific scaffold was obtained by decellularization of human pancreatic fragments. The viability and functional activity of rat islets isolated with collagenase were determined. Experimental T1D was modeled by intraperitoneal injection of low-dose streptozotocin and incomplete Freund’s adjuvant into rats. The rats were intraperitoneally injected twice with pancreatic CEC (n = 2) or islet suspension (n = 1). Glucose levels in the blood and urine of the rats were assessed. Histological examination of organs (pancreas and kidneys) of the experimental animals was carried out.Results. After the first injection, blood glucose levels gradually decreased in all animals by more than 47% of the initial values; by follow-up day 24, the glucose level rose to the initial hyperglycemic values. After repeated administration, a 63.4% decrease in glycemic level was observed in the rats with pancreatic CEC and a 47.5% decrease in the one with islet suspension. At week 5 of the experiment, blood glucose levels gradually increased in all animals. At the same time, the glycemic index of the rat with injected pancreatic CEC was 62% lower than the glycemic index of the rat with injected islets.Conclusion. Allogeneic pancreatic islets in pancreatic CEC increase the duration of stable glycemic level in T1D rats.
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- 2023
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33. Personalized Medicine for IBD Patients
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Fadeeva, Nina, Khatkov, Igor, Bodunova, Natalia, Knyazev, Oleg, Bordin, Dmitry, Parfenov, Asfold, Nikolskaya, Karina, Nikolaev, Sergey, Rumyantsev, Konstantin, Polyakova, Vera, and Yanova, Tatiana
- Published
- 2023
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34. CLARIFICATION TO 'COMPARATIVE ANALYSIS OF INDICATORS OF ECONOMIC EFFICIENCY OF CULTIVATION OF CHERRY SEEDLINGS WITH DRIP IRRIGATION' DOI: 10.12731/2658-6649-2024-16-1-722
- Author
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Olga A. Nikolskaya, Elena V. Seminchenko, and Andrey V. Solonkin
- Subjects
clarification ,Agriculture ,Science - Abstract
Nikolskaya O.A., Semenchenko E.V., Solonkin A.V. Comparative Analysis of Indicators of Economic Efficiency of Cultivation of Cherry Seedlings with Drip Irrigation. Siberian Journal of Life Sciences and Agriculture, 2024, vol. 16, no. 1, pp. 102-118. DOI: 10.12731/2658-6649-2024-16-1-722 The authors clarify the source of funding: The research was carried out within the framework of the state assignment of research work of the Federal Research Center of Agroecology of the Russian Academy of Sciences № 122020100448-6 “Creation of new competitive forms, varieties and hybrids of cultivated, woody and shrub plants with high productivity, quality and increased resistance to adverse environmental factors, new innovative technologies in seed and nursery production taking into account varietal characteristics and soil and climatic conditions of arid territories of the Russian Federation” The original article can be found under DOI: https://doi.org/10.12731/2658-6649-2024-16-1-722
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- 2024
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35. Effect of Post-Implantation Heat Treatment Conditions on Photoluminescent Properties of Ion-Synthesized Gallium Oxide Nanocrystals
- Author
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Dmitry S. Korolev, Kristina S. Matyunina, Alena A. Nikolskaya, Alexey I. Belov, Alexey N. Mikhaylov, Artem A. Sushkov, Dmitry A. Pavlov, and David I. Tetelbaum
- Subjects
gallium oxide ,nanocrystals ,ion synthesis ,ion implantation ,thermal annealing ,photoluminescence ,Chemistry ,QD1-999 - Abstract
A novel and promising way for creating nanomaterials based on gallium oxide is the ion synthesis of Ga2O3 nanocrystals in a SiO2/Si dielectric matrix. The properties of nanocrystals are determined by the conditions of ion synthesis—the parameters of ion irradiation and post-implantation heat treatment. In this work, the light-emitting properties of Ga2O3 nanocrystals were studied depending on the temperature and annealing atmosphere. It was found that annealing at a temperature of 900 °C leads to the appearance of intense luminescence with a maximum at ~480 nm caused by the recombination of donor–acceptor pairs. An increase in luminescence intensity upon annealing in an oxidizing atmosphere is shown. Based on data from photoluminescence excitation spectroscopy and high-resolution transmission electron microscopy, a hypothesis about the possibility of the participation of a quantum-size effect during radiative recombination is proposed. A mechanism for the formation of Ga2O3 nanocrystals during ion synthesis is suggested, which makes it possible to describe the change in the luminescent properties of the synthesized samples with varying conditions of post-implantation heat treatment.
- Published
- 2024
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36. Obtaining and Purifying the Recombinant Domain III of Human Alpha-Fetoprotein
- Author
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Yabbarov, N. G., Mollaev, M. D., Zabolotskii, A. I., Mazalev, D. A., Gorokhovets, N. V., Sokol, M. B., Mollaeva, M. R., Fomicheva, M. V., Pshenichnikova, A. B., and Nikolskaya, E. D.
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- 2022
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37. CHARACTERISTICS OF OLOKIZUMAB PHARMACOKINETICS IN PATIENTS WITH NOVEL CORONAVIRUS INFECTION COVID-19
- Author
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E. V. Tavlueva, E. V. Zernova, M. P. Kutepova, N. E. Kostina, V. S. Lesina, D. R. Mould, K. Ito, A. V. Zinchenko, A. N. Dolgorukova, M. V. Nikolskaya, M. S. Lemak, O. V. Filon, and M. Yu. Samsonov
- Subjects
covid-19 ,olokizumab ,clearance ,pharmacokinetic model ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The aim of the article is to study pharmacokinetic characteristics of intravenous olokizumab in patients with moderate COVID-19 to relieve a hyperinflammation syndrome.Materials and methods. The pharmacokinetic study was conducted as a part of a phase III clinical study (RESET, NCT05187793) on the efficacy and safety of a new olokizumab regimen (intravenous, at the doses of 128 mg or 256 mg) in COVID-19 patients. Plasma concentrations of olokizumab were determined by the enzyme immunoassay. The population analysis was performed using a previously developed pharmacokinetic model based on a linear two compartment.Results. The pharmacokinetic analysis included the data from 8 moderate COVID-19 patients who had been administrated with olokizumab intravenously at the dose of 128 mg. According to the analysis results in this population, there was an increase in the drug clearance, compared with the data obtained in healthy volunteers and the patients with rheumatoid arthritis: 0.435, 0.178 and 0.147 l/day, respectively. The parameters analysis within the framework of a population pharmacokinetic model showed that the main factors for the increased olokizumab clearance are a high body mass index. In addition, the presence of COVID-19 itself is an independent factor in increasing the drug clearance.Conclusion. After the intravenous olokizumab administration, an increase in the drug clearance is observed in moderate COVID-19 patients against the background of the disease course. The main contribution to the increased clearance is made by the characteristics of the population of COVID-19 patients associated with the risk of a severe disease and inflammation. When administered intravenously at the dose of 128 mg, a therapeutically significant olokizumab level was maintained throughout the acute disease phase for 28 days.
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- 2022
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38. Apoptotic bone marrow-derived mononuclear cells accelerate liver regeneration after extended resection
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N. A. Onishchenko, A. O. Nikolskaya, Z. Z. Gonikova, L. A. Kirsanova, M. Yu. Shagidulin, and V. I. Sevastianov
- Subjects
apoptotic bone marrow cells ,regeneration ,liver resection ,Surgery ,RD1-811 - Abstract
Objective: to compare the efficiency of regenerative processes in the liver using apoptotic bone marrow-derived mononuclear cells (BMMCs) and intact BMMCs from healthy animals on an extended liver resection (ELR) model.Materials and methods. Male Wistar rats (n = 77) with an ELR model (70–75%) were divided into 3 groups: group 1 (control with a single intraperitoneal injection of saline), group 2 (single intraperitoneal injection of unsorted intact BMMCs at a dose of 30–35 × 106, and group 3 (single intraperitoneal injection of apoptotic BMMCs at the same dose). Restoration of biochemical parameters of liver function and mass, as well as the emerging microstructural changes in hepatocytes in histological preparations, were monitored by assessing hepatocyte mitotic activity (MA) during the first 7–10 days after ELR.Results. It was found that in groups 2 and 3, as compared with group 1, there was no death after ELR modeling, and that the biochemical parameters of liver function normalized more rapidly (at days 10–14). Hepatocyte MA in group 3 sharply increased as early as on day 1, and mitotic index (MI) averaged 14‰, reaching 20.9‰ in some experiments; MI in the control group remained at the baseline by this time, while in group 2, MI was only 3.2‰. In group 3, liver mass recovered more rapidly after ELR to baseline values already at days 8–10, whereas the recovery was at day 12–14 and day 17–20 in group 2 and group 1, respectively. It was suggested that the more pronounced increase in the efficiency of regenerative processes in the liver after ELR in group 3 after using apoptotic BMMCs was due to the release from these cells of a large spectrum of formed paracrine factors, including various classes of RNA molecules involved in the regeneration process.Conclusion. Apoptotic BMMNCs have a more effective adaptive and regulatory potential than intact BMMCs because reorganizations are rapidly formed in the damaged liver cells, providing an early and more powerful activation of the targeted regenerative program.
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- 2022
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39. Treatment of chronic liver disease using cell‑engineered constructs: morphofunctional characteristics
- Author
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M. Yu. Shagidulin, N. A. Onishchenko, A. V. Grechina, M. E. Krasheninnikov, A. O. Nikolskaya, E. A. Volkova, N. P. Mogeiko, N. A. Boiarinova, A. V. Lyundup, G. A. Piavchenko, L. I. Davydova, A. Yu. Arhipova, V. G. Bogush, and S. V. Gautier
- Subjects
regenerative medicine ,chronic liver disease treatment ,matrix ,microgel ,liver cells ,bone marrow-derived mesenchymal stem cells ,cell-engineered constructs ,Surgery ,RD1-811 - Abstract
Objective: to study the effectiveness of correcting the morphofunctional characteristics of the liver in an experimental model of chronic liver disease (CLD), using implanted cell-engineered constructs (CECs).Materials and methods. Experiments were carried out on male Wistar rats (n = 80) aged 6–8 months with an initial weight of 230–250 g. CLD was modeled by inoculating the rats with 60% CCl4 oil solution for 42 days based on a modified scheme. Microgel based on recombinant spidroin rS1/9 was used as a matrix for CECs fabrication. Allogeneic liver cells (LCs) and multipotent bone marrow-derived mesenchymal stem cells (BM-MSCs) from a healthy donor were used as the cellular component of the CECs. The effectiveness of the corrective effect of the implanted CECs was assessed in an experimental CLD model (n = 60) in two groups of rats: Group 1 (control, n = 20, 1 mL of saline solution was injected into the damaged liver parenchyma) and Group 2 (experimental, n = 40, CECs containing allogenic LCs and BM-MSCs in a 5 : 1 ratio in a volume of 1 mL were implanted into the damaged liver parenchyma). For long-term monitoring of the CEC state, the CECs were labeled by additional inclusion in Cytodex-3. The effectiveness of the regulatory effect of CECs on regenerative processes in the liver was evaluated using biochemical, morphological and morphometric techniques, as well as by flow cytometry at 90 days after implantation.Results. In the control group, the mortality rate in CLD was 25%. There was no death in the experimental group with CLD after CEC implantation. The CECs were found to have a corrective effect on the biochemical and morphological parameters of the liver in CLD during 90 days of follow-up, with concomitant preservation of structural cellular homeostasis in the implanted CECs. Conclusion. Implantation of CECs in the liver facilitates effective correction of CLD by activating regenerative processes in the damaged liver, which is due to long-term preservation of structural cellular homeostasis in the CECs.
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- 2022
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40. The Advantages of Combining Drip Irrigation with Growth-Stimulating Fertilizing When Growing Annual Cherry Seedlings
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Kruzhilin, I. P. and Nikolskaya, O. A.
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- 2022
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41. Analysis of the marginal adaptation of temporary filling materials to tooth tissues
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A. V. Makonin, I. S. Kopetskiy, I. A. Nikolskaya, L. V. Pobozhieva, J. V. Shevelyuk, A. A. Khritova, and I. A. Shalaev
- Subjects
проницаемость временных пломбировочных материалов ,временные пломбировочные материалы ,герметичные свойства стоматологических материалов ,Dentistry ,RK1-715 - Abstract
Aim. To evaluate the marginal fit of temporary filling materials used in practice of a dentist-therapist.Materials and methods. The analysis of the degree of marginal fit of temporary sealing materials was carried out: light polymerization-«Tempofot liquid-flowing» (VladMiVa), «Restorin liquid-flowing (Technodent)», «Tempofot packable» (VladMiVa) and chemical curing – «Parasept» (VladMiVa), «Dentin paste» (Vladmiva), «Tempodent» (Technodent). The study was conducted on 120 removed teeth.Results. After 1 day of exposure, the materials «Dentin paste», «Tempodent» and «Tempofot packable» were penetrated by the hyposalix solution along the filling-tooth border. It can be stated that these materials have no hermetic properties. Thematerial «Parasept» and the materials «Tempofot liquid-flowing», «Restorin liquid-flowing» retained their hermetic properties, there was no staining of the filling-wall border of the tooth. After 3 days, the materials «Parasept» and «Tempofot liquid-flowing» staining was not detected, the material «Restorin liquid-flowing», the penetration of the dye to the mouth of the root canals was noted.Conclusions. The study found that temporary sealing materials of chemical and light polymerization have various hermetic properties.
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- 2022
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42. Pharmaceutical Approach to Develop Novel Photosensitizer Nanoformulation: An Example of Design and Characterization Rationale of Chlorophyll α Derivative
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Maria B. Sokol, Veronika A. Beganovskaya, Mariia R. Mollaeva, Nikita G. Yabbarov, Margarita V. Chirkina, Dmitry V. Belykh, Olga M. Startseva, Anton E. Egorov, Alexey A. Kostyukov, Vladimir A. Kuzmin, Sergei M. Lomakin, Natalia G. Shilkina, Alexey V. Krivandin, Olga V. Shatalova, Margarita A. Gradova, Maxim A. Abakumov, Aleksey A. Nikitin, Varvara P. Maksimova, Kirill I. Kirsanov, and Elena D. Nikolskaya
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photodynamic therapy ,photosensitizer ,nanoparticles ,PLGA ,chlorophyll derivative ,antitumor activity ,Pharmacy and materia medica ,RS1-441 - Abstract
In this study, we described physico-chemical properties of novel nanoformulation of photosensitizer-pyropheophorbide α 17-diethylene glycol ester (XL) (chlorophyll α derivative), revealing insights into antitumor activity and maintaining quality, meeting the pharmaceutical approach of new nanoformulation design. Our formulation, based on poly(lactic-co-glycolic acid) (PLGA) nanoparticles, increased XL solubility and selective tumor-targeted accumulation. In our research, we revealed, for the first time, that XL binding to polyvinyl alcohol (PVA) enhances XL photophysical activity, providing the rationale for PVA application as a stabilizer for nanoformulations. Results of FTIR, DSC, and XRD revealed the physical interactions between XL and excipients, including PVA, indicating that the encapsulation maintained XL binding to PVA. The encapsulated XL exhibited higher photophysical activity compared to non-encapsulated substance, which can be attributed to the influence of residual PVA. Gamma-irradiation led to degradation of XL; however, successful sterilization of the samples was achieved through the filtration. Importantly, the encapsulated and sterilized XL retained cytotoxicity against both 2D and 3D tumor cell models, demonstrating the potential of the formulated NP–XL for photodynamic therapy applications, but lacked the ability to reactivate epigenetically silenced genes. These findings provide valuable insights into the design and characterization of PLGA-based nanoparticles for the encapsulation of photosensitizers.
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- 2024
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43. Programmed cell death and liver diseases
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N. A. Onishchenko, Z. Z. Gonikova, A. O. Nikolskaya, L. A. Kirsanova, and V. I. Sevastianov
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programmed cell death ,autophagy ,apoptosis ,necrosis ,liver diseases ,Surgery ,RD1-811 - Abstract
Cell death represents the most critical pathologic entity in liver disease, which dictates pathologic consequences such as inflammation, fibrosis, and cell transformation. We analyzed the conclusions of studies on the involvement of different types of programmed cell death (PCD) in the pathogenesis of liver diseases. Three main forms of PCD (autophagy, apoptosis, necrosis) and five additional, still insufficiently studied PCD – necroptosis, ferroptosis, pyroptosis, partanatosis and entosis – observed in the liver in various acute and chronic diseases are considered. The involvement of several PCD at once in the development of any one pathology and one type of PCD in different pathologies was established. This indicates the existence of cross-regulation of metabolism in the liver cells with different levels of damage in the formation of the main dominant type of PCD. Available results indicate the possibility of attenuation (correction) of functional and morphological manifestations of PCD in the organ by controlled blocking of effector-mediated PCD pathways, as well as targeted induction of autophagy, anti-apoptotic and anti-necrotic mechanisms in liver cells.
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- 2022
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44. Effect of transdermal immunomodulation on liver regeneration
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E. G. Kuznetsova, O. M. Kuryleva, L. A. Salomatina, L. A. Kirsanova, Z. Z. Gonikova, A. O. Nikolskaya, N. P. Shmerko, and V. I Sevastianov
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transdermal therapeutic system ,sodium aminodihydrophthalazinedione ,immunomodulator ,extended liver resection ,mitotic index ,Surgery ,RD1-811 - Abstract
Introduction. The use of immunomodulators to regulate reparative processes in affected organs and tissues remains a pressing issue. Of greatest interest is liver regeneration after extended hepatic resection (EHR) in donors in right lobe living related donor liver transplantation. We propose a transdermal therapeutic system (TTS) with an immunomodulator to enhance the natural process of liver tissue regeneration. Objective: to study the effect of transdermal administration of immunomodulator sodium aminodihydrophthalazinedione on early recovery processes in the liver after EHR in in vivo experiments.Materials and methods. Sodium aminodihydrophthalazinedione was used as an active substance in TTS in the form of powder for preparation of intramuscular injection solution (Galavit®, SELVIM LLC). An experimental EHR model was performed on 22 male Wistar rats weighing 350–380 g. After HER, all animals were divided into two groups. Group 1 (n = 10) consisted of untreated animals. In group 2 (n = 12), TTS was applied immediately after liver resection. The experiment lasted for 48 hours; the TTS was changed once after 24 hours from the beginning of application.Results. In either group, there was no significant difference in the weight of liver remnant gain and in biochemical blood parameters at 48 hours after EHR. Assessment of the mitotic index (MI) of hepatocytes 48 hours after EHR revealed a significant increase in MI in both groups in comparison with the baseline (before liver resection) equal to 0.14 ± 0.07‰. The MI in group 1 and group 2 animals was 12.70 ± 4.9‰ and 17.43 ± 4.90‰, respectively (p ≤ 0.05).Conclusion. Studies on the regenerative activity of sodium aminodihydrophthalazinedione TTS on an experimental EHR model in rats showed that this drug form had a pronounced stimulating effect on the mitotic activity of liver cells.
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- 2022
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45. Torque change of anterior teeth based on CBCT using a universal table
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I. S. Kopetskiy, N. G. Meskhiya, A. I. Kopetskaya, I. A. Nikolskaya, and G. S. Kabisova
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ортодонтическое лечение ,толщина костной ткани ,клкт ,центр сопротивления ,торк зуба ,Dentistry ,RK1-715 - Abstract
Aim. The thickness of bone tissue in patients with pathological dental inclinations planning orthodontic treatment with fixed equipment.Materials and methods. The CBCT scans of patients with pathological dental inclinations was studied, bone tissue deficiency was described, a universal table was given that helps to calculate the necessary thickness of bone tissue at various levels of root length at the stage of treatment planning when the torc of the frontal teeth changes.Results. Bone tissue deficiency is described in two study groups, however, in patients with retrusion, the deficiency is most pronounced in the cervical third from the vestibular side, in the group with protrusion, the deficiency is described in the cervical third from the oral surface in the cervical third.Conclusions. The article describes a clinical case of orthodontic treatment using a universal table. The principle of operation of the universal table is shown, as well as its own clinical results proving the effectiveness of the universal table are described.
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- 2022
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46. Phylogenomic analysis of the diversity of graspetides and proteins involved in their biosynthesis
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Kira S. Makarova, Brittney Blackburne, Yuri I. Wolf, Anastasia Nikolskaya, Svetlana Karamycheva, Marlene Espinoza, Clifton E. Barry, Carole A. Bewley, and Eugene V. Koonin
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RiPPs ,ATP-grasp ligase ,Graspetides ,Biosynthetic gene clusters ,Lactones ,Lactams ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Bacteria and archaea produce an enormous diversity of modified peptides that are involved in various forms of inter-microbial conflicts or communication. A vast class of such peptides are Ribosomally synthesized, Postranslationally modified Peptides (RiPPs), and a major group of RiPPs are graspetides, so named after ATP-grasp ligases that catalyze the formation of lactam and lactone linkages in these peptides. The diversity of graspetides, the multiple proteins encoded in the respective Biosynthetic Gene Clusters (BGCs) and their evolution have not been studied in full detail. In this work, we attempt a comprehensive analysis of the graspetide-encoding BGCs and report a variety of novel graspetide groups as well as ancillary proteins implicated in graspetide biosynthesis and expression. Results We compiled a comprehensive, manually curated set of graspetides that includes 174 families including 115 new families with distinct patterns of amino acids implicated in macrocyclization and further modification, roughly tripling the known graspetide diversity. We derived signature motifs for the leader regions of graspetide precursors that could be used to facilitate graspetide prediction. Graspetide biosynthetic gene clusters and specific precursors were identified in bacterial divisions not previously known to encode RiPPs, in particular, the parasitic and symbiotic bacteria of the Candidate phyla radiation. We identified Bacteroides-specific biosynthetic gene clusters (BGC) that include remarkable diversity of graspetides encoded in the same loci which predicted to be modified by the same ATP-grasp ligase. We studied in details evolution of recently characterized chryseoviridin BGCs and showed that duplication and horizonal gene exchange both contribute to the diversification of the graspetides during evolution. Conclusions We demonstrate previously unsuspected diversity of graspetide sequences, even those associated with closely related ATP-grasp enzymes. Several previously unnoticed families of proteins associated with graspetide biosynthetic gene clusters are identified. The results of this work substantially expand the known diversity of RiPPs and can be harnessed to further advance approaches for their identification.
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- 2022
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47. Phylogenomic analysis of the diversity of graspetides and proteins involved in their biosynthesis
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Makarova, Kira S., Blackburne, Brittney, Wolf, Yuri I., Nikolskaya, Anastasia, Karamycheva, Svetlana, Espinoza, Marlene, Barry, III, Clifton E., Bewley, Carole A., and Koonin, Eugene V.
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- 2022
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48. The role of apoptotic bone marrow cells in activation of liver regeneration
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N. A. Onishchenko, A. O. Nikolskaya, Z. Z. Gonikova, L. A. Kirsanova, M. Yu. Shagidulin, and V. I. Sevastianov
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apoptosis ,autophagy ,bone marrow cells ,liver ,resection ,regeneration ,Surgery ,RD1-811 - Abstract
Objective: using an adoptive transfer model to study the cellular mechanisms involved in the formation of the initial stage of liver regeneration during intraperitoneal injection of a healthy recipient with apoptotic bone marrowderived mononuclear cells (BM-MNCs) from a donor after extended liver resection.Materials and methods. Male Wistar rats (n = 40) were used to create a model of adoptive transfer of apoptotic BM-MNCs (a-BM-MNCs) taken from the donor after extended liver resection to a healthy recipient. During the experiments, the animals were divided into five groups. Four experimental groups with intraperitoneal injection of the same doses to the recipient: freshly isolated BM-MNCs (group 1); BM-MNCs subjected to apoptosis for 48 hours by storage at t = 4–6 °C in phosphate-buffered saline (PBS) (group 2) or in a Custodiol HTK solution (group 3). In group 4, the animals were injected with PBS after storing BM-MNCs in it. The control animals were animals injected with saline (group 5). For selection of effective modes of apoptosis induction, BM-MNCs stained with 7AAD after incubation in solutions were analyzed by flow cytometry. Targeted transfer of regenerative signals to the recipient was assessed by the mitotic activity of hepatocytes in the liver and tubular epithelium in the kidneys, as well as by the intensity of microstructural changes in the liver 24, 48 and 72 hours after injection of the studied material.Results. BMC incubation in PBS and HTK for 48 hours at t = 4–6 °C provides the most effective accumulation of a-BM-MNCs in early apoptosis. It was shown that a-BM-MNCs retain the ability to target-focused transmission of regulatory signals to the liver supported by autophagy process during adoptive transfer. It was established that a-BM-MNCs (groups 2 and 3) in comparison to native BM-MNCs (group 1) at adoptive transfer increased the regenerative potential of the liver due to pronounced increase in the activity of autophagy processes and directed infiltration of immunomodulatory mononuclear cells in the liver.Conclusion. a-BM-MNCs create a stronger basis for development and implementation of a targeted and effective regeneration program by enhancing autophagy processes and immunomodulatory effect on mononuclear cells, which are regenerative signal carriers.
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- 2022
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49. The Main Strategic Directions of the Education System Development (on the Example of Higher Education Institutions on Personnel Education for the Hospitality Industry)
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Zaitseva, Natalia A., Ilina, Elena L., Nikolskaya, Elena Y., Romanova, Marianna M., and Larionova, Anna A.
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The importance and relevance of research of questions is determined by the intensive development of the tourism and hospitality industry, the consequence of which is the increase of business needs for skilled personnel. The purpose of this paper is to examine and assess the main strategic trends in educational technologies and methods used for education of qualified personnel for the hospitality industry. For analysis of the modern educational processes in the hospitality industry the authors of the article have used theoretical and empirical research methods, economic-statistical and factor analysis, expert evaluations. In the article the conclusions about the quality of Russian education are made, the main trends in change of system of personnel education by the Russian higher education institutions are proved, including those in which personnel education for the hospitality industry is conducted. The main strategic directions of Russian education development are determined, the European experience of education for the hospitality industry is summarized, the main tendencies of the development of education system for the hospitality industry in Europe are formulated. Moreover, the application of professional standards in the Russian system of education for the hospitality industry is proved, and a draft of "road map" of the development of higher education system (on the example of personnel education for the hospitality industry) until 2025 is developed.
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- 2016
50. Development of the Composition and Technology for Obtaining Paclitaxel Nanoscale Formulation Consisting of a Conjugate of Polymer Particles with a Protein Vector Molecule
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M. B. Sokol, N. G. Yabbarov, M. R. Mollaeva, M. V. Chirkina (Fomicheva), V. Yu. Balaban'yan, and E. D. Nikolskaya
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nanoparticles ,plga ,paclitaxel ,controlled release ,alpha-fetoprotein ,Pharmaceutical industry ,HD9665-9675 - Abstract
Introduction. The use of the anticancer drug paclitaxel is limited due to its high toxicity and lipophilicity. A new polymer composition of paclitaxel has been proposed, which provides targeted transport of the drug into tumor cells and improves its safety.Aim. Method development for preparation of a novel paclitaxel formulation consisting of a conjugate of PLGA nanoparticles with the third domain of alpha-fetoprotein.Materials and methods. The object of this study is paclitaxel-loaded nanoparticles based on a copolymer of lactic and glycolic acids, the surface of which is modified with a vector molecule - the recombinant third domain of alpha-fetoprotein. Nanoparticles were obtained by single emulsification method and precipitation. Conjugation with a protein molecule was performed by the carbodiimide method. The analysis of the obtained nanoparticles was carried out using dynamic and electrophoretic light scattering, high performance liquid chromatography, dialysis membrane method.Results and discussion. Synthesis of paclitaxel-loaded nanoparticles based on a copolymer of lactic and glycolic acids and its conjugation optimization under varying a wide range of conditions have been carried out. The resulting conjugate had an average diameter of 280 ± 12 nm. The conjugation efficiency was 95 %. The release of paclitaxel from the polymer matrix in the release medium was 65 % in 220 h.Conclusions. A method of obtaining and substantiating the composition of the original nanosized form of paclitaxel is proposed. The possibility of prolonged release of paclitaxel from the polymer matrix has been shown.
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- 2021
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