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15. Specific Recognition of Arginine Methylated Histone Tails by JMJD5 and JMJD7.

Author

Liu, Haolin, Wang, Chao, Lee, Schuyler, Ning, Fangkun, Wang, Yang, Zhang, Qianqian, Chen, Zhongzhou, Zang, Jianye, Nix, Jay, Dai, Shaodong, Marrack, Philippa, Hagman, James, Kappler, John, and Zhang, Gongyi

Subjects
Animals, Humans, Mice, Arginine, Histones, Crystallography, X-Ray, Protein Processing, Post-Translational, Protein Conformation, Protein Binding, Methylation, Models, Molecular, Histone Demethylases, Jumonji Domain-Containing Histone Demethylases, Proteolysis, Crystallography, X-Ray, Protein Processing, Post-Translational, Models, Molecular, Biochemistry and Cell Biology, Other Physical Sciences
Abstract

We have reported that JMJD5 and JMJD7 (JMJD5/7) are responsible for the clipping of arginine methylated histone tails to generate "tailless nucleosomes", which could release the pausing RNA polymerase II (Pol II) into productive transcription elongation. JMJD5/7 function as endopeptidases that cleave histone tails specifically adjacent to methylated arginine residues and continue to degrade N-terminal residues of histones via their aminopeptidase activity. Here, we report structural and biochemical studies on JMJD5/7 to understand the basis of substrate recognition and catalysis mechanism by this JmjC subfamily. Recognition between these enzymes and histone substrates is specific, which is reflected by the binding data between enzymes and substrates. High structural similarity between JMJD5 and JMJD7 is reflected by the shared common substrates and high binding affinity. However, JMJD5 does not bind to arginine methylated histone tails with additional lysine acetylation while JMJD7 does not bind to arginine methylated histone tails with additional lysine methylation. Furthermore, the complex structures of JMJD5 and arginine derivatives revealed a Tudor domain-like binding pocket to accommodate the methylated sidechain of arginine, but not lysine. There also exists a glutamine close to the catalytic center, which may suggest a unique imidic acid mediated catalytic mechanism for proteolysis by JMJD5/7.

Published
2018

32. Clipping of arginine-methylated histone tails by JMJD5 and JMJD7

Author

Liu, Haolin, Wang, Chao, Lee, Schuyler, Deng, Yu, Wither, Matthew, Oh, Sangphil, Ning, Fangkun, Dege, Carissa, Zhang, Qianqian, Liu, Xinjian, Johnson, Aaron M., Zang, Jianye, Chen, Zhongzhou, Janknecht, Ralf, Hansen, Kirk, Marrack, Philippa, Li, Chuan-Yuan, Kappler, John W., Hagman, James, and Zhang, Gongyi

Published
2017

43. Electromagnetic shielding property of duplex magnesium alloy with laminated structure processed by accumulative roll bonding

Author

Qichi Le, Niu Yanxia, Hou Jian, Zhang Qinglong, and Ning Fangkun

Subjects
010302 applied physics, Materials science, Polymers and Plastics, Alloy, Metals and Alloys, Oxide, 02 engineering and technology, engineering.material, 021001 nanoscience & nanotechnology, Microstructure, 01 natural sciences, Electromagnetic radiation, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Roll bonding, Biomaterials, Accumulative roll bonding, chemistry.chemical_compound, chemistry, 0103 physical sciences, Electromagnetic shielding, engineering, Magnesium alloy, Composite material, 0210 nano-technology
Abstract

To solve the gradually serious electromagnetic pollution problem, more and more electromagnetic shielding alloys are designed because of its higher conductivities and better workability. However, the increasing incident electromagnetic frequency with the more powerful energy enhance the electromagnetic wave penetrates ability and relatively weakens the alloys' shielding properties. Some researches indicate that the shielding material with laminated structure exhibit better shielding property at higher incident electromagnetic frequency. Accordingly, a novel duplex Mg-9Li alloy with laminated structure of 24-1 interfaces of strips for electromagnetic shielding was designed and fabricated by accumulative roll bonding (ARB) up to 4 cycles at ambient temperature. With the analysis of microstructure evolution and bonding mechanism, the relative conductivity and the density of the alloy are decreased from 15.38% to 12.03% and from 100% to 94%, respectively. Such changes are attributed to insulted oxide or contaminant during roll bonding. The shielding effectiveness (SE) of the sheets are increased from 56 dB to 73 dB at 1350 MHz with the increasing of phase interlayers interface through ARB process which can be ascribed to the laminated structure of Mg-Li alloy processed by ARB shielding properties according to shielding mechanism.

Published
2018
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44. 1,25D3 prevents CD8+Tc2 skewing and asthma development through VDR binding changes to the Cyp11a1 promoter

Author

Schedel, Michaela, primary, Jia, Yi, additional, Michel, Sven, additional, Takeda, Katsuyuki, additional, Domenico, Joanne, additional, Joetham, Anthony, additional, Ning, Fangkun, additional, Strand, Matthew, additional, Han, Junyan, additional, Wang, Meiqin, additional, Lucas, Joseph J., additional, Vogelberg, Christian, additional, Kabesch, Michael, additional, O’Connor, Brian P., additional, and Gelfand, Erwin W., additional

Published
2016
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45. The structural basis of urea-induced protein unfolding in β-catenin

Author

Wang, Chao, primary, Chen, Zhongzhou, additional, Hong, Xia, additional, Ning, Fangkun, additional, Liu, Haolin, additional, Zang, Jianye, additional, Yan, Xiaoxue, additional, Kemp, Jennifer, additional, Musselman, Catherine A., additional, Kutateladze, Tatinna G., additional, Zhao, Rui, additional, Jiang, Chengyu, additional, and Zhang, Gongyi, additional

Published
2014
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46. Structural characterization of the pulmonary innate immune protein SPLUNC1 and identification of lipid ligands

Author

Ning, Fangkun, primary, Wang, Chao, additional, Berry, Karin Zemski, additional, Kandasamy, Pitchaimani, additional, Liu, Haolin, additional, Murphy, Robert C., additional, Voelker, Dennis R., additional, Nho, Chu Won, additional, Pan, Choel‐Ho, additional, Dai, Shaodong, additional, Niu, Liwen, additional, Chu, Hong‐Wei, additional, and Zhang, Gongyi, additional

Published
2014
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50. Mesenchymal Stem Cells Recruit CCR2 + Monocytes To Suppress Allergic Airway Inflammation.

Author

Takeda K, Webb TL, Ning F, Shiraishi Y, Regan DP, Chow L, Smith MJ, Ashino S, Guth AM, Hopkins S, Gelfand EW, and Dow S

Subjects
Animals, Cell Movement immunology, Female, Inflammation immunology, Inflammation metabolism, Mesenchymal Stem Cells metabolism, Mice, Mice, Inbred C57BL, Receptors, CCR2 biosynthesis, Respiratory Hypersensitivity metabolism, Mesenchymal Stem Cells immunology, Monocytes immunology, Receptors, CCR2 immunology, Respiratory Hypersensitivity immunology
Abstract

Mesenchymal stem cells (MSC) exert immune modulatory properties and previous studies demonstrated suppressive effects of MSC treatment in animal models of allergic airway inflammation. However, the underlying mechanisms have not been fully elucidated. We studied the role of MSC in immune activation and subsequent recruitment of monocytes in suppressing airway hyperresponsiveness and airway inflammation using a mouse model of allergic airway inflammation. MSC administration prior to or after allergen challenge inhibited the development of airway inflammation in allergen-sensitized mice. This was accompanied by an influx of CCR2-positive monocytes, which were localized around injected MSC in the lungs. Notably, IL-10-producing monocytes and/or macrophages were also increased in the lungs. Systemic administration of liposomal clodronate or a CCR2 antagonist significantly prevented the suppressive effects of MSC. Activation of MSC by IFN-γ leading to the upregulation of CCL2 expression was essential for the suppressive effects, as administration of wild-type MSC into IFN-γ-deficient recipients, or IFN-γ receptor-deficient or CCL2-deficient MSC into wild-type mice failed to suppress airway inflammation. These results suggest that MSC activation by IFN-γ, followed by increased expression of CCL2 and recruitment of monocytes to the lungs, is essential for suppression by MSC in allergen-induced airway hyperresponsiveness and airway inflammation., (Copyright © 2018 by The American Association of Immunologists, Inc.)

Published
2018
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