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Onions (Allium cepa L.) contain various flavonols, including quercetin, kaempferol, anthocyanin, luteolin, and myricetin. Quercetin in onions is considered the primary bioactive component. To assess the impact of quercetin on hyperuricemia in healthy Wistar albino rats, this study used high‐performance liquid chromatography with ultraviolet (HPLC‐UV) to identify and measure quercetin in onion powder. Twenty‐four 160 ± 10 g, six wistar albino male rats in each group were kept: NC (control sample, no onion powder), OT1, OT2, and OT3, which contained 11.13, 14.84, and 18.61 g/100 g onion powder, respectively. The treatment lasted 28 days, during which the last 7 days were for urine, feces, and blood collection. The results showed a trend of decreasing levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, total bilirubin, total cholesterol, and low‐density lipoprotein in rats fed OT1, OT2, and OT3 diets. Improvements were observed in feed, water, and nutrient intake, feed conversion ratio, feed efficiency ratio, nutrient digestibility, nitrogen balance, body weight, blood urea nitrogen, creatinine, and uric acid levels (p ≤.05). In contrast, high‐density lipoprotein, triglycerides, serum total protein, neutrophils, and lymphocytes did not change (p ≥.05). White blood cells, red blood cell count, platelet count, hemoglobin, and monocytes showed an upward trend. Based on our calculations, we determined the optimal human dosage from the most effective amount of onion powder. By taking into account the ratio of human‐to‐rat surface area, we estimate that the equivalent human dose of onion is 181.04 grams with 204 mg of quercetin. Additionally, when factoring in the dry matter content, the recommended dose of onion is 29.19 grams with 220 mg of quercetin. [ABSTRACT FROM AUTHOR]

Antigenicity of buffalo alpha S1 (α-S1) casein and its hydrolysis by gastrointestinal enzymes were carried out in this study.α-S1 casein was purified by anion exchange chromatography and hydrolyzed for 150 min. Indirect Enzyme-linked immunosorbent assays were performed using sera of groups of patients allergic to cow milk and healthy group of subjects non-allergic to cow milk. The patients allergic to cow milk showed significant response toα-S1 casein, which reduced with higher degree of hydrolysis. Maximum reduction of antigenicity was observed for trypsin at 85%. Similarly,α- chymotrypsin at pH 7.8, pepsin at pH 2.2 and pH 5.5 reduced the antigenicity to 63%, 60% and 38%, respectively. The enzymatic hydrolysis can reduce the antigenicity of buffaloα-S1 casein in patients allergic to cow milk. However, a complete inhibition of immunogenic reaction requires supplementary treatment beyond gastrointestinal enzymes. [ABSTRACT FROM AUTHOR]

Jambo grass (Sorghum bicolour × Sorghum sudanefe) harvested 50 days after sowing was ensiled in laboratory silos using 2 additives, cane molasses and ground corn grains, each at the rate of 2%, 4%, and 6% levels for 30, 35, and 40 days at room temperature (28 °C). Data regarding incubation days were pooled. The pH and lactic acid concentration in jambo grass silage were not affected by the additive type, level, or fermentation period. Dry matter (DM) content of jambo silage was significantly (P < 0.05) affected by additive type; however, additive levels and fermentation periods did not affect its DM contents. Crude protein content of jambo grass was not affected by ensiling time, additive type, or level. The true protein contents of jambo grass silage were significantly (P < 0.05) higher with molasses than with ground corn. Neutral detergent fiber (NDF) and hemicellulose content of jambo grass were significantly (P < 0.05) affected by additives and their levels, but fermentation periods did not influence the NDF contents of jambo grass silage. Cellulose, acid detergent fiber, and acid detergent lignin contents of jambo silage were not affected by additive type, level, or fermentation period. Therefore, jambo grass ensiled with 2% molasses for 30 days was selected for in situ digestion kinetics in bulls. Dry matter and NDF degradabilities of jambo grass were significantly higher than those of its silage at 48 h of ruminal incubation. Ruminal lag time, and rate and extent of DM and NDF degradation were similar for jambo grass and its silage. The results of this study implied that jambo grass ensiled with 2% molasses for 30 days has similar nutritive value to that of its forage in bulls. [ABSTRACT FROM AUTHOR]

The present work was conceptualized to determine the potential protective effects of curcumin on arsenic-induced kidney damage in male albino rat model. Thirty six male albino rats were selected, weighed about 175±10g and classified into four groups (9 rats in each group) such as C group (control with basal diet), Cur group (curcumin 200mg/kg body weight), AI group (arsenic-induced 5mg/kg body weight) and AI + Cur group (arsenic 5mg/kg+curcumin 200mg/kg body weight), respectively. Arsenic and curcumin were offered through the gavage method once daily with basal diet. The different analyzed parameters showed that arsenic-induced elevation of aspartate amino transferase, alkaline phosphatase, bilirubin urea, alanine aminotransferase and creatinine significantly decreased with curcumin application in AI + Cur group. Similarly, the statistically significant decline of low-density lipoprotein (LDL), cholesterol, triglyceride and increased in high-density lipoprotein (HDL) was observed in rats of AI + Cur group with curcumin treatment as compared to the rats of AI group. The level of different enzymes of the liver as well as kidney was noted depleted on arsenic exposure whereas increased in level was observed with curcumin application in AI + Cur group. Moreover, pathological histology changes were also recorded. The outcomes suggest that curcumin has a potential effect against arsenic-induced toxicity in biological model.

The objective of this study to evaluate the effect of Resistant Starch (RS) on lipid profile, blood glucose, hormones and TNF-α in Type 2 Diabetes (T2DM). 45 Wister male rats were divided into 5 groups each having 9 rats: without T2DM negative control (NC) with T2DM positive control (PC), resistant starch: 0.20g/kg body weight (HM 0.20 ), resistant starch: 0.30g/kg body weight (HM 0.30 ) and resistant starch: 0.40g/kg body weight (HM 0.40 ). Different levels were added in basal diets while negative and positive control received basal diet. Animals fed on different diets were induced type 2 diabetes 60mg/kg streptozotocin solution injected intraperitoneally in rats. Results of blood glucose showed significantly (P<0.05) lower in all rats fed HM 0.20 , HM 0.30 and HM 0.40 . In case of lipid profile results indicated that HM0.04 showed significant (P<0.05) increase in HDL level while cholesterol, LDL, triglycerides were reduced. After treatment hormonal profile, leptin, insulin and TNF-α level were significantly (P<0.05) reduced. It may be concluded that RS is the new approach to treat the T2DM.

Background: Cardiovascular diseases and diabetes are responsible for large number of deaths throughout the globe. Bitter gourd has the potential to become a component of the diet or a dietary supplement for diabetic and pre-diabetic patients owing to the presence of insulin like molecules. Recent investigations have suggested that bitter gourd extracts may ameliorate high fat diet induced obesity and hyperlipidemia in animal models. Moreover, its supplements in food result in lowering weight gain and visceral fat mass., Methods: The current study was designed to investigate the nutraceutical potential of skin, flesh and whole fruit of bitter gourd cultivars against hyperglycemia and hyperlipidemia. For the purpose, various bitter gourd cultivars were procured from local market. Bio-evaluation studies were carried out on biochemical parameters using rodent experiment model., Results: From results, it was revealed that maximum reduction in blood glucose skin 1.06%, flesh 2.65%, whole fruit 4.29%, total cholesterol skin 6.60%, flesh 6.04%, whole fruit 6.70%, low density lipoprotein skin 5.55%, flesh 6.81%, whole fruit 6.60%, and triglycerides skin 0.04%, flesh 3.38%, whole fruit 2.02%, were observed. Moreover, insulin skin 2.14%, flesh 3.52%, whole fruit 2.73%, production was slightly enhanced with improved levels of high density lipoprotein in whole fruit of bitter gourd., Conclusion: Overwhelmingly, it may be inferred here that bitter gourd holds the potential to significantly improve diabetic conditions and associated late complications with no ill effects on body organs.