Search

Your search keyword '"Nishiyama, Kumiko"' showing total 27 results
Start Over Author "Nishiyama, Kumiko"
27 results on '"Nishiyama, Kumiko"'

5. Modulation of Ca2+ oscillation following ischemia and nicotinic acetylcholine receptors in primary cortical neurons by high-throughput analysis.

Author

Sasaki, Tsutomu, Hisada, Sunao, Kanki, Hideaki, Nunomura, Kazuto, Lin, Bangzhong, Nishiyama, Kumiko, Kawano, Tomohito, Matsumura, Shigenobu, and Mochizuki, Hideki

Subjects
NICOTINIC acetylcholine receptors, FREQUENCIES of oscillating systems, NEURON analysis, CEREBRAL ischemia, METHYL aspartate receptors, NICOTINIC receptors
Abstract

Calcium oscillations in primary neuronal cultures and iPSCs have been employed to investigate arrhythmogenicity and epileptogenicity in drug development. Previous studies have demonstrated that Ca2+ influx via NMDA and nicotinic acetylcholine receptors (nAChRs) modulates Ca2+ oscillations. Nevertheless, there has been no comprehensive investigation into the impact of ischemia or nAChR-positive allosteric modulators (PAM) drugs on Ca2+ oscillations at a level that would facilitate high-throughput screening. We investigated the effects of ischemia and nAChR subtypes or nAChR PAM agonists on Ca2+ oscillations in high-density 2D and 3D-sphere primary neuronal cultures using 384-well plates with FDSS-7000. Ischemia for 1 and 2 h resulted in an increase in the frequency of Ca2+ oscillations and a decrease in their amplitude in a time-dependent manner. The NMDA and AMPA receptor inhibition significantly suppressed Ca2+ oscillation. Inhibition of NR2A or NR2B had the opposite effect on Ca oscillations. The potentiation of ischemia-induced Ca2+ oscillations was significantly inhibited by the NMDA receptor antagonist, MK-801, and the frequency of these oscillations was suppressed by the NR2B inhibitor, Ro-256981. In the 3D-neurosphere, the application of an α7nAChR agonist increased the frequency of Ca2+ oscillations, whereas the activation of α4β2 had no effect. The combination of nicotine and PNU-120596 (type II PAM) affected the frequency and amplitude of Ca2+ oscillations in a manner distinct from that of type I PAM. These systems may be useful not only for detecting epileptogenicity but also in the search for neuroprotective agents against cerebral ischemia. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

9. The tight junction protein occludin modulates blood-brain barrier integrity and neurological function after ischemic stroke in mice

Author

Sugiyama, Shintaro, primary, Sasaki, Tsutomu, additional, Tanaka, Hiroo, additional, Yan, Haomin, additional, Ikegami, Takeshi, additional, Kanki, Hideaki, additional, Nishiyama, Kumiko, additional, Beck, Goichi, additional, Gon, Yasufumi, additional, Okazaki, Shuhei, additional, Todo, Kenichi, additional, Tamura, Atsushi, additional, Tsukita, Sachiko, additional, and Mochizuki, Hideki, additional

Published
2022
Full Text
View/download PDF

14. New 5-Aryl-Substituted 2-Aminobenzamide-Type HDAC Inhibitors with a Diketopiperazine Group and Their Ameliorating Effects on Ischemia-Induced Neuronal Cell Death

Author

Hirata, Yoshiyuki, primary, Sasaki, Tsutomu, additional, Kanki, Hideaki, additional, Choong, Chi-Jing, additional, Nishiyama, Kumiko, additional, Kubo, Genki, additional, Hotei, Ayana, additional, Taniguchi, Masahiko, additional, Mochizuki, Hideki, additional, and Uesato, Shinichi, additional

Published
2018
Full Text
View/download PDF

20. 成人男女健常者における体脂肪率(%Fat)とWaist/Hip比(WHR)の関係の性差

Author

Tahara, Yasuaki, Tsunawake, Noriaki, Yukawa, Kouichi, Yamasaki, Masahiro, Nishiyama, Kumiko, Urata, Hideko, Katsuno, Kumiko, and Fukuyama, Yumiko

Subjects
BMI, Waist-to-stature ratio (WAS), Waist-to-hip ratio (WHR), Percent body fat (%Fat), population characteristics, nutritional and metabolic diseases, Body composition
Abstract

The purpose of the present study was to investigate sexual differences in relationships among percent body fat (%Fat), waist-to-hip ratio (WHR), waist-to-stature ratio (WSR), abdominal circumference to stature ratio (ASR), body mass Index (BMI) and skinfold thicknesses in healthy male and female adults. Subjects were 64 males and 65 females, aged 22-60. Body density was measured by under \vater weighing and by skinfold anthropometry. Mean %Fat was 15.6% in males and 23.9% in females. Mean WHR was 0.83 in males and 0.72 in females. The correlation between %Fat and WHR was not significant in females (r= -0.l04) but was significant in males (r= 0.631, p 0.001). Highly significant correlations were obtained among %Fat, WSR, ASR, BMI, and sum of eight skinfolds in both sexes.

Published
1994

22. ADRs for sale!

Author

De Verneuil, Vanina, Nishiyama, Kumiko, Seem, Alan, Ikeda, Masahisa, and Ozawa, Joji

Subjects
DISPUTE resolution, FOREIGN corporations, SECURITIES industry, LISTING of securities, INVESTORS, FINANCIAL disclosure, FINANCIAL markets
Abstract

The article focuses on how a foreign private issuer can protect itself from becoming a target of alternative dispute resolution (ADR) due to the changes in the U.S. Rule 12g3-2(b) securities regulation. The U.S. Securities and Exchange Commission (SEC) adopted amendments to Rule 12g3-2(b), promulgated under the U.S. Securities Exchange Act of 1934, with the intention to improve the trading liquidity of non-U.S. companies and make it easier for U.S. investors to gain access to a foreign private issuer's non-U.S. disclosure documents. It informs that under the foreign listing condition, the foreign private issuer must maintain a listing of equity securities on one or more non-U.S. exchanges constituting the primary trading market for the securities.

Published
2009

23. This title is unavailable for guests, please login to see more information.

Author

Tahara, Yasuaki, Tsunawake, Noriaki, Yukawa, Kouichi, Yamasaki, Masahiro, Nishiyama, Kumiko, Urata, Hideko, Katsuno, Kumiko, Fukuyama, Yumiko, Tahara, Yasuaki, Tsunawake, Noriaki, Yukawa, Kouichi, Yamasaki, Masahiro, Nishiyama, Kumiko, Urata, Hideko, Katsuno, Kumiko, and Fukuyama, Yumiko

Abstract

The purpose of the present study was to investigate sexual differences in relationships among percent body fat (%Fat), waist-to-hip ratio (WHR), waist-to-stature ratio (WSR), abdominal circumference to stature ratio (ASR), body mass Index (BMI) and skinfold thicknesses in healthy male and female adults. Subjects were 64 males and 65 females, aged 22-60. Body density was measured by under \vater weighing and by skinfold anthropometry. Mean %Fat was 15.6% in males and 23.9% in females. Mean WHR was 0.83 in males and 0.72 in females. The correlation between %Fat and WHR was not significant in females (r= -0.l04) but was significant in males (r= 0.631, p 0.001). Highly significant correlations were obtained among %Fat, WSR, ASR, BMI, and sum of eight skinfolds in both sexes.

24. Driver gene KRAS aggravates cancer-associated stroke outcomes

Author

Yan, Haomin, Sasaki, Tsutomu, Gon, Yasufumi, Nishiyama, Kumiko, Kanki, Hideaki, Mochizuki, Hideki, Yan, Haomin, Sasaki, Tsutomu, Gon, Yasufumi, Nishiyama, Kumiko, Kanki, Hideaki, and Mochizuki, Hideki

Abstract

Yan Haomin, Sasaki Tsutomu, Gon Yasufumi, et al. Driver gene KRAS aggravates cancer-associated stroke outcomes. Thrombosis Research 233, 55 (2023); https://doi.org/10.1016/j.thromres.2023.11.015., The incidence of cancer-associated stroke has increased with the prolonged survival times of cancer patients. Recent genetic studies have led to progress in cancer therapeutics, but relationships between oncogenic mutations and stroke remain elusive. Here, we focused on the driver gene KRAS, which is the predominant RAS isoform mutated in multiple cancer types, in cancer associated stroke study. KRASG13D/− and parental human colorectal carcinoma HCT116 cells were inoculated into mice that were then subjected to a photochemically-induced thrombosis model to establish ischemic stroke. We found that cancer inoculation exacerbated neurological deficits after stroke. Moreover, mice inoculated with KRASG13D/− cells showed worse neurological deficits after stroke compared with mice inoculated with parental cells. Stroke promoted tumor growth, and the KRASG13D/− allele enhanced this growth. Brain RNA sequencing analysis and serum ELISA showed that chemokines and cytokines mediating pro-inflammatory responses were upregulated in mice inoculated with KRASG13D/− cells compared with those inoculated with parental cells. STAT3 phosphorylation was promoted following ischemic stroke in the KRASG13D/− group compared with in the parental group, and STAT3 inhibition significantly ameliorated stroke outcomes by mitigating microglia/macrophage polarization. Finally, we compared the prognosis and mortality of colorectal cancer patients with or without stroke onset between 1 January 2007 and 31 December 2020 using a hospital-based cancer registry and found that colorectal cancer patients with stroke onset within 3 months after cancer diagnosis had a worse prognosis. Our work suggests an interplay between KRAS and ischemic stroke that may offer insight into future treatments for cancer-associated stroke.

25. Driver gene KRAS aggravates cancer-associated stroke outcomes

Author

Yan, Haomin, Sasaki, Tsutomu, Gon, Yasufumi, Nishiyama, Kumiko, Kanki, Hideaki, Mochizuki, Hideki, Yan, Haomin, Sasaki, Tsutomu, Gon, Yasufumi, Nishiyama, Kumiko, Kanki, Hideaki, and Mochizuki, Hideki

Abstract

Yan Haomin, Sasaki Tsutomu, Gon Yasufumi, et al. Driver gene KRAS aggravates cancer-associated stroke outcomes. Thrombosis Research 233, 55 (2023); https://doi.org/10.1016/j.thromres.2023.11.015., The incidence of cancer-associated stroke has increased with the prolonged survival times of cancer patients. Recent genetic studies have led to progress in cancer therapeutics, but relationships between oncogenic mutations and stroke remain elusive. Here, we focused on the driver gene KRAS, which is the predominant RAS isoform mutated in multiple cancer types, in cancer associated stroke study. KRASG13D/− and parental human colorectal carcinoma HCT116 cells were inoculated into mice that were then subjected to a photochemically-induced thrombosis model to establish ischemic stroke. We found that cancer inoculation exacerbated neurological deficits after stroke. Moreover, mice inoculated with KRASG13D/− cells showed worse neurological deficits after stroke compared with mice inoculated with parental cells. Stroke promoted tumor growth, and the KRASG13D/− allele enhanced this growth. Brain RNA sequencing analysis and serum ELISA showed that chemokines and cytokines mediating pro-inflammatory responses were upregulated in mice inoculated with KRASG13D/− cells compared with those inoculated with parental cells. STAT3 phosphorylation was promoted following ischemic stroke in the KRASG13D/− group compared with in the parental group, and STAT3 inhibition significantly ameliorated stroke outcomes by mitigating microglia/macrophage polarization. Finally, we compared the prognosis and mortality of colorectal cancer patients with or without stroke onset between 1 January 2007 and 31 December 2020 using a hospital-based cancer registry and found that colorectal cancer patients with stroke onset within 3 months after cancer diagnosis had a worse prognosis. Our work suggests an interplay between KRAS and ischemic stroke that may offer insight into future treatments for cancer-associated stroke.

26. REGULATORY CAPITAL IS BROKEN.

Author

Fournier, Elizabeth, De Cordova, Pamela Gonzales, James, Benedict, Miles, Allegra, Crompton, Simon, Faith, Jo, Gleeson, Simon, Blackmore, Vanessa, Evans, Rachel, Siskey, Kyle, De Verneuil, Vanina, Nishiyama, Kumiko, Seem, Alan, Ikeda, Masahisa, Ozawa, Joji, Stender, Neal, Zhou, Lin Lawrence, Zeng, Yan, Elliott, Giles, and Irani, Vica

Subjects
BASEL II (2004), BANKING industry, FINANCIAL crises, FINANCIAL leverage, RISK management in business
Abstract

The author comments on the Basel II accord of the Basel Committee for Banking Practice. She expresses concern on Basel II and states that, it exacerbates market cycles and encourages economic boom and bust. She states that the committee should regulate the risk assessment operations of rating agencies. The committee needs to impose a leverage ratio restriction alongside risk-sensitive capital adequacy requirements to get a better picture of bank operations.

Published
2008

27. Two-dimensional site frequency spectrum for detecting, classifying and dating incomplete selective sweeps.

Author

Satta Y, Zheng W, Nishiyama KV, Iwasaki RL, Hayakawa T, Fujito NT, and Takahata N

Subjects
Asian People, Data Interpretation, Statistical, Genetic Drift, Genome, Human, Humans, Linkage Disequilibrium, Mutation, Polymorphism, Single Nucleotide, Recombination, Genetic, Alleles
Abstract

The two-dimensional site frequency spectrum (2D SFS) was investigated to describe the intra-allelic variability (IAV) maintained within a derived allele (D) group that has undergone an incomplete selective sweep against an ancestral allele group. We observed that recombination certainly muddles the ancestral relationships of allelic lineages between the two allele groups; however, the 2D SFS reveals intriguing signatures of recombination as well as the genealogical structure of the D group, particularly the size of a mutation and the time to the most recent common ancestor (TMRCA). Coalescent simulations were performed to achieve powerful and robust 2D SFS-based statistics with special reference to accurate evaluation of IAV, significance of recombination effects, and distinction between hard and soft selective sweeps. These studies were extended to a case wherein an incomplete selective sweep is no longer in progress and ceased in the recent past. The 2D SFS-based method was applied to 100 intronic linkage disequilibrium regions randomly chosen from the East Asian population of modern humans to examine the P value distributions of the summary statistics under the null hypothesis of neutrality in a nonequilibrium demographic model. We argue that about 96% of intronic variants are non-adaptive with a 10% false discovery rate. Furthermore, this method was applied to six genomic regions in Eurasian populations that were claimed to have experienced recent selective sweeps. We found that two of these genomic regions did not have significant signals of selective sweeps, but the remaining four had undergone hard and soft sweeps and were dated, in terms of TMRCA, after the major out-of-Africa dispersal of modern humans.

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results