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Fine‐grained authigenic magnetite has been recognized increasingly in iron‐rich marine environments affected by methane seepage and is a major sedimentary magnetization source. However, it is unknown whether this magnetite forms via microbial or abiotic processes. We report here abundant fine magnetite crystals, in close association with goethite, within coarse‐grained sediments from two adjacent methane seepage sites in the South China Sea. The magnetite‐ and goethite‐rich horizons have sharply increased Zr/Ti, Zr/Rb, Ti/Al, and Fe/Al ratios, probably reflecting deposition by turbidity currents. Deeper intervals have elevated pyrite content, positive δ34S excursions of chromium reducible sulfur, and low magnetic susceptibilities, which is consistent with past sulfate‐driven anaerobic oxidation of methane in environments with dynamically variable seepage intensity. In magnetically extracted aggregates (>63 μm), magnetite particles are mainly clustered euhedral crystals with 0.2–0.8 μm sizes, which will likely impact sedimentary magnetic signals. The fine, euhedral crystalline nature of the magnetite suggests formation in sulfide‐free, ferrous iron‐rich sedimentary environments. Based on 16S rRNA gene sequences, anaerobic methanotrophic archaea coincide with pyrite rich horizons. In contrast, two co‐occurring methanogenic archaea groups of the Methanomicrobia class (mainly Methanosarcina and Methanocella) are particularly abundant in turbidites but have low abundance in all other horizons. Increased Methanomicrobia abundances suggest that this class of archaea may be involved in microbial iron reduction in turbidites with abundant goethite as a reactive iron source, and that they apparently trigger magnetite formation. Our findings provide new clues to microbial magnetite formation in iron‐rich marine sediments. Plain Language Summary: Magnetite is a key source of sedimentary magnetic signals. Understanding the origin of magnetite in sediments is critical for proper interpretation of paleomagnetic records. Fine‐grained authigenic magnetite has been recognized increasingly in iron‐rich continental margin environments with high methane flux and represents a further important magnetization source in marine sediments. However, it is unknown whether this magnetite forms via microbial or abiotic processes. Here, we identify abundant fine magnetite crystals within iron‐rich subseafloor horizons from two adjacent methane seepage sites in the South China Sea. From magnetic, mineralogical, geochemical, and genomic analyses, we provide new constraints for understanding magnetite formation in iron‐rich sediments, where methanogenic archaea apparently perform microbial iron reduction and trigger magnetite formation. Key Points: We identify abundant fine magnetite crystals within subseafloor iron‐rich layers from methane seepage impacted sitesMethanogenic archaea coincide with magnetite‐rich horizons and are considered potential drivers of microbial iron reduction and magnetite formationOur findings have profound implications for the nature of paleomagnetic signals recorded in iron‐rich sediments, and for iron cycling in marine sediments [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of music on heart rate recovery (HRR) and heart rate variability (HRV) after intense exertion. Methods: Five hundred male students enrolled at Yongin University, Korea, underwent a cycling test to assess aerobic capacity; 180 students with equal scores were selected for a music intervention, which was conducted after vigorous exercise. The 180 participants were randomized into three music groups and a control group; the participants in each music group listened to music at three different tempos: slow (lento) (n = 45), moderate (moderato) (n = 45), and fast (allegretto) (n = 45). The control group did not listen to music (n = 45). After the test, data on cardiac recovery and HRV were gathered and modeled. Results: The results revealed no significant variation in HRR and HRV indexes between the four cohorts (p >.05), and no significant differences were observed in the anaerobic power cycling indexes during strenuous exercise (p >.05). The music intervention had a significant impact on HR, low‐frequency power (LF), high‐frequency power (HF), normalized LF (LFnorm), normalized HF (HFnorm), and the LF/HF ratio during recovery (p <.05). Conclusion: After rigorous activity, listening to allegretto music improved HRR and restored HRV equilibrium, which is critical to preventing and minimizing arrhythmias and sudden cardiac death. [ABSTRACT FROM AUTHOR]

Marine cold seeps are unique chemosynthetic habitats fuelled by deeply sourced hydrocarbon‐rich fluids discharged at the seafloor. Through oxidizing methane and other hydrocarbons, microorganisms inhabiting cold seeps supply subsurface‐derived energy to higher trophic levels, sustaining highly productive oases of life in the deep sea. Despite the central role of microbiota in mediating biogeochemical cycles, the factors that govern the assembly and network of prokaryotic communities in cold seeps remain poorly understood. Here we analysed the geochemical and microbiological profiles of 11 different sediment cores from two spatially distant cold seeps of the South China Sea. We show that prokaryotic communities belonging to the same methane‐supply regimes (high‐methane‐supply, low‐methane‐supply and non‐seep control sediments) had a highly similar community structure, regardless of geographical location, seep‐associated biota (mussel, clam, microbial mat) and sediment depth. Methane supply appeared to drive the niche partitioning of anaerobic methanotrophic archaea (ANME) at the regional scale, with ANME‐1 accounting for >60% sequence abundance of ANME in the high‐methane‐supply sediments, while ANME‐2 dominated (>90%) the low‐methane‐supply sediments. Increasing methane supply enhanced the contribution of environmental selection but lessened the contributions of dispersal limitation and drift to overall community assembly. High methane supply, moreover, promoted a more tightly connected, less stable prokaryotic network dominated by positive correlations. Together, these results provide a potentially new framework for understanding the niches and network interplay of prokaryotic communities across different methane seepage regimes in cold‐seep sediments. [ABSTRACT FROM AUTHOR]

In marine sediments, microorganisms are known to play important roles in nitrogen cycling; however, the composition and quantity of microbes taking part in each process of nitrogen cycling are currently unclear. In this study, two different types of marine sediment samples (shallow bay and deep-sea sediments) in the South China Sea (SCS) were selected to investigate the microbial community involved in nitrogen cycling. The abundance and composition of prokaryotes and seven key functional genes involved in five processes of the nitrogen cycle [nitrogen fixation, nitrification, denitrification, dissimilatory nitrate reduction to ammonium (DNRA), and anaerobic ammonia oxidation (anammox)] were presented. The results showed that a higher abundance of denitrifiers was detected in shallow bay sediments, while a higher abundance of microbes involved in ammonia oxidation, anammox, and DNRA was found in the deep-sea sediments. Moreover, phylogenetic differentiation of bacterial amoA, nirS, nosZ, and nrfA sequences between the two types of sediments was also presented, suggesting environmental selection of microbes with the same geochemical functions but varying physiological properties. [ABSTRACT FROM AUTHOR]

Denitrification is a crucial process in the global nitrogen cycle, in which two functionally equivalent genes, nirS and nirK, catalyse the critical reaction and are usually used as marker genes. The nirK gene can function independently, whereas nirS requires additional genes to encode nitrite reductase and is more sensitive to environmental factors than nirK. However, the ecological differentiation mechanisms of those denitrifying microbial communities and their adaptation strategies to environmental stresses remain unclear. Here, we conducted metagenomic analysis for sediments and bioreactor samples from Lake Donghu, China. We found that nirS‐type denitrifying communities had a significantly lower horizontal gene transfer frequency than that of nirK‐type denitrifying communities, and nirS gene phylogeny was more congruent with taxonomy than that of nirK gene. Metabolic reconstruction of metagenome‐assembled genomes further revealed that nirS‐type denitrifying communities have robust metabolic systems for energy conservation, enabling them to survive under environmental stresses. Nevertheless, nirK‐type denitrifying communities seemed to adapt to oxygen‐limited environments with the ability to utilize various carbon and nitrogen compounds. Thus, this study provides novel insights into the ecological differentiation mechanism of nirS and nirK‐type denitrifying communities, as well as the regulation of the global nitrogen cycle and greenhouse gas emissions. [ABSTRACT FROM AUTHOR]

Expression of high mobility group protein box 1 (HMGB1) in children with respiratory syncytial virus bronchiolitis and its effect on the inflammatory function of monocytes were investigated. A total of 30 cases of respiratory syncytial viral bronchitis and 30 cases of healthy persons from physical examination were collected from January 2017 to September 2019 in the pediatric department of Xuzhou Children's Hospital, Xuzhou Medical University. HMGB1 expression level in plasma was detected by ELISA. All participants in the study were followed up for 18 months. Human recombinant respiratory syncytial virus (RSV)-A2 virus was used to infect human bronchial epithelial cell line 16HBE, and cell culture supernatant was collected to detect HMGB1. Transwell plate was used to co-culture infected or no-infection groups of epithelial cells and monocytes THP-1. Western blot was used to detect the level of Toll-like receptor (TLR)4 and TLR7 in monocytes. HMGB1 expression level in peripheral blood of children with bronchiolitis was significantly increased compared with that in healthy controls (P<0.0001), and was significantly correlated with the severity of the children's condition (P<0.01). The expression level of HMGB1 was significantly correlated with the number of monocytes, lymphocytes and CRP expression level. HMGB1 was also significantly increased in cell culture supernatant compared with no-infection group (P<0.0001). TLR4 expression in monocytes could be activated by the virus infected cell lines. Follow-up results showed that children with bronchiolitis had a higher incidence of asthma within 18 months (P<0.05). The independent risk factors for children to develop asthma were age, number of monocytes and HMGB1 level. HMGB1 is highly expressed in peripheral blood of children with respiratory syncytial virus bronchitis, and RSV epithelial cells can activate TLR4 expression in monocytes, suggesting that HMGB1 plays an important role in monocyte mediated immune inflammation. HMGB1 expression level is related to the development of asthma in children, which is of great significance for understanding the pathogenesis of bronchiolitis and suggesting the prognosis of children., (Copyright: © Niu et al.)