1. Mangiferin potentiates the activity of antifungal agents against fluconazole-resistant Candida spp.
- Author
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Ferreira TL, Leitão AC, da Silva LJ, do Amaral Valente Sá LG, de Farias Cabral VP, Rodrigues DS, Barbosa SA, de Andrade Neto JB, Barbosa AD, Almeida Moreira LE, França Rios ME, Cavalcanti BC, de Moraes MO, Nobre Júnior HV, and da Silva CR more...
- Subjects
- Humans, Apoptosis drug effects, Candida albicans drug effects, Azoles pharmacology, Antifungal Agents pharmacology, Xanthones pharmacology, Fluconazole pharmacology, Microbial Sensitivity Tests, Drug Synergism, Biofilms drug effects, Drug Resistance, Fungal drug effects, Amphotericin B pharmacology, Candida drug effects
- Abstract
Aim: To evaluate the antifungal activity of mangiferin against Candida spp. resistant to fluconazole. Materials & methods: The antifungal activity of mangiferin was assessed using broth microdilution and its interaction with azoles and amphotericin B was evaluated by checkerboard. The activity of mangiferin against Candida spp. biofilms was assessed using the MTT colorimetric assay and its possible mechanism of action was evaluated using flow cytometry. Results: Mangiferin showed activity against Candida albicans, Candida tropicalis and Candida parapsilosis resistant to fluconazole and showed synergism with azoles and amphotericin B. Mangiferin increased the activity of antifungals against Candida biofilms and caused depolarization of the mitochondrial membrane and externalization of phosphatidylserine, suggesting apoptosis. Conclusion: mangiferin combined with antifungals has potential against Candida spp. more...
- Published
- 2024
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