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164 results on '"Nocca, Giuseppina"'

4. Human Serum Albumin Is an Essential Component of the Host Defense Mechanism Against Clostridium difficile Intoxication

Author

di Masi, Alessandra, Leboffe, Loris, Polticelli, Fabio, Tonon, Federica, Zennaro, Cristina, Caterino, Marianna, Stano, Pasquale, Fischer, Stephan, Hägele, Marlen, Müller, Martin, Kleger, Alexander, Papatheodorou, Panagiotis, Nocca, Giuseppina, Arcovito, Alessandro, Gori, Andrea, Ruoppolo, Margherita, Barth, Holger, Petrosillo, Nicola, Ascenzi, Paolo, and Di Bella, Stefano

Published
2018

5. Cinnamaldehyde Loaded Poly(lactide-co-glycolide) (PLGA) Microparticles for Antifungal Delivery Application against Resistant Candida albicans and Candida glabrata

Author

Rizzo, Silvia, primary, Di Vito, Maura, additional, Mazzinelli, Elena, additional, Favuzzi, Ilaria, additional, Torelli, Riccardo, additional, Cacaci, Margherita, additional, Arcovito, Alessandro, additional, Sanguinetti, Maurizio, additional, Garzoli, Stefania, additional, Nocca, Giuseppina, additional, and Bugli, Francesca, additional

Published
2023
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6. Oral Mucosa Models to Evaluate Drug Permeability

Author

Mazzinelli, Elena, primary, Favuzzi, Ilaria, additional, Arcovito, Alessandro, additional, Castagnola, Raffaella, additional, Fratocchi, Giorgia, additional, Mordente, Alvaro, additional, and Nocca, Giuseppina, additional

Published
2023
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10. Circulating and Salivary NGF and BDNF Levels in SARS-CoV-2 Infection: Potential Predictor Biomarkers of COVID-19 Disease—Preliminary Data

Author

Biamonte, Filippo, primary, Re, Agnese, additional, Balzamino, Bijorn Omar, additional, Ciasca, Gabriele, additional, Santucci, Daniela, additional, Napodano, Cecilia, additional, Nocca, Giuseppina, additional, Fiorita, Antonella, additional, Marino, Mariapaola, additional, Basile, Umberto, additional, Micera, Alessandra, additional, and Callà, Cinzia Anna Maria, additional

Published
2022
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12. Piezoelectric Device Versus Conventional Osteotomy Instruments in the Comparison of Three Different Bone Harvesting Methods: An Istomorphometric, Phonometric, and Chronometric Evaluation.

Author

Valente, Nicola Alberto, Cosma, Lorenzo, Nocca, Giuseppina, D'Addona, Antonio, and Lajolo, Carlo

Subjects
PIEZOSURGERY, OSTEOTOMY, SURGICAL instruments, JAW surgery, CELL proliferation, BONE grafting, CELL physiology, SCANNING electron microscopy
Abstract

Purpose: This study was conducted to compare the bone cutting performance of the piezoelectric device with traditional drills in terms of cut execution time, environmental noise produced, surface morphology characteristics, and residual cell vitality of the bone samples. Materials and Methods: Two fresh pig jaws were obtained from a local slaughterhouse, and nine bone samples with a standard size (1 cm3) were harvested from each jaw: three using piezosurgery, three with a multiblade bur mounted on a surgical handpiece, and three with a diamond bur mounted on a high-speed handpiece, for a total of 18 samples. Two samples for each harvesting method were examined by scanning electron microscope (SEM), observing four surfaces per sample. For each surface observed, a count of the intertrabecular spaces was performed, and each space was evaluated as completely, partially, or unfilled by debris. Four samples per sampling method were examined by cell culture to evaluate residual cell vitality after cutting. The execution time of each osteotomy was measured with a stopwatch. The environmental noise was measured, at two different distances, with a phonometer. Results: At SEM analysis, piezosurgery osteotomies showed 66% of totally free intertrabecular spaces on the cutting surface, statistically significantly higher than those on the multiblade bur (33%) and diamond bur (12%) cutting surfaces (P < .0005). Bone samples harvested with piezosurgery also demonstrated faster cell proliferation. Finally, piezosurgery generated less environmental noise, though it required longer cutting time. Conclusion: Piezoelectric technology is a valid alternative to rotating burs for osteotomy, demonstrating higher bone cell viability and a precise and silent, though slower, cut. [ABSTRACT FROM AUTHOR]

Published
2019
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18. Recent Advances in the Label-Free Characterization of Exosomes for Cancer Liquid Biopsy: From Scattering and Spectroscopy to Nanoindentation and Nanodevices

Author

Di Santo, Riccardo, primary, Romanò, Sabrina, additional, Mazzini, Alberto, additional, Jovanović, Svetlana, additional, Nocca, Giuseppina, additional, Campi, Gaetano, additional, Papi, Massimiliano, additional, De Spirito, Marco, additional, Di Giacinto, Flavio, additional, and Ciasca, Gabriele, additional

Published
2021
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21. Fourier Transform Infrared Spectroscopy as a useful tool for the automated classification of cancer cell-derived exosomes obtained under different culture conditions

Author

Romanò, Sabrina, primary, Di Giacinto, Flavio, additional, Primiano, Aniello, additional, Mazzini, Alberto, additional, Panzetta, Claudia, additional, Papi, Massimiliano, additional, Di Gaspare, Alessandra, additional, Ortolani, Michele, additional, Gervasoni, Jacopo, additional, De Spirito, Marco, additional, Nocca, Giuseppina, additional, and Ciasca, Gabriele, additional

Published
2020
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23. Biocompatibility of Orthodontic Resins: In vitro Evaluation of Monomer Leaching and Cytotoxic Effects

Author

UOMO, ROBERTO, SPAGNUOLO, GIANRICO, Nocca, Giuseppina, GALEOTTI, Angela, MICHELOTTI, AMBROSINA, VALLETTA, ROSA, D'ANTO', VINCENZO, Uomo, Roberto, Spagnuolo, Gianrico, Nocca, Giuseppina, Galeotti, Angela, Michelotti, Ambrosina, Valletta, Rosa, and D'Anto', Vincenzo

Abstract

The aim of this study was to investigate the effect of orthodontic resins on cell survival and to evaluate monomer leaching both before and after resin polymerization. Materials and methods: 3T3 mouse fibroblasts were exposed to three cured and uncured orthodontic resins. Cellular viability was assessed by Alamar Blue assay after 24, 48 and 72 h. High Performance Liquid Chromatography was used to measure the amount of monomers released by the tested samples. Data were analyzed by means of ANOVA and Tukey’s test (p

Published
2017

25. A protein chimera self‐assembling unit for drug delivery

Author

Amalfitano, Adriana, primary, Martini, Cecilia, additional, Nocca, Giuseppina, additional, Papi, Massimiliano, additional, De Spirito, Marco, additional, Sanguinetti, Maurizio, additional, Vitali, Alberto, additional, Bugli, Francesca, additional, and Arcovito, Alessandro, additional

Published
2018
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27. Biosynthesis of innovative calcium phosphate/hydrogel composites: physicochemical and biological characterisation.

Author

Chronopoulou, Laura, Cacciotti, Ilaria, Amalfitano, Adriana, Nitto, Antonio Di, D'Arienzo, Veronica, Nocca, Giuseppina, and Palocci, Cleofe

Subjects
NUCLEAR magnetic resonance spectroscopy, CALCIUM phosphate, HYDROGELS, FOURIER transform infrared spectroscopy, FIELD emission electron microscopy, BIOMIMETIC materials, DIFFERENTIAL scanning calorimetry
Abstract

The goal of supporting and directing tissue regeneration requires the design of new, advanced materials, with features like biocompatibility, biodegradability and adequate mechanical properties. Our work was focused on developing a new injectable biomimetic composite material, based on a peptidic hydrogel and calcium phosphates with the aim of mimicking the chemical composition of natural bone tissue. Arg-Gly-Asp-grafted chitosan was used to promote cell adhesion. The obtained composite hydrogel was characterized with differential scanning calorimetry measurements, rheological analysis, field emission scanning electron microscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance measurements. The biological responsiveness was assessed using the MG-63 human osteoblast cell line. [ABSTRACT FROM AUTHOR]

Published
2021
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29. Controlled release of 18-β-glycyrrhetic acid by nanodelivery systems increases cytotoxicity on oral carcinoma cell line

Author

Cacciotti, Ilaria, primary, Chronopoulou, Laura, additional, Palocci, Cleofe, additional, Amalfitano, Adriana, additional, Cantiani, Monica, additional, Cordaro, Massimo, additional, Lajolo, Carlo, additional, Callà, Cinzia, additional, Boninsegna, Alma, additional, Lucchetti, Donatella, additional, Gallenzi, Patrizia, additional, Sgambato, Alessandro, additional, Nocca, Giuseppina, additional, and Arcovito, Alessandro, additional

Published
2018
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39. Human Serum Albumin Is an Essential Component of the Host Defense Mechanism Against Clostridium difficile Intoxication.

Author

Masi, Alessandra di, Leboffe, Loris, Polticelli, Fabio, Tonon, Federica, Zennaro, Cristina, Caterino, Marianna, Stano, Pasquale, Fischer, Stephan, Hägele, Marlen, Müller, Martin, di Masi, Alessandra, Kleger, Alexander, Papatheodorou, Panagiotis, Nocca, Giuseppina, Arcovito, Alessandro, Gori, Andrea, Ruoppolo, Margherita, Barth, Holger, Petrosillo, Nicola, and Ascenzi, Paolo

Subjects
CLOSTRIDIOIDES difficile, PROTEINS, TOXINS, CYTOSKELETON, SERUM albumin, ADENOCARCINOMA, ORGANOIDS
Abstract

Background: The pathogenic effects of Clostridium difficile are primarily attributable to the production of the large protein toxins (C difficile toxins [Tcd]) A (TcdA) and B (TcdB). These toxins monoglucosylate Rho GTPases in the cytosol of host cells, causing destruction of the actin cytoskeleton with cytotoxic effects. Low human serum albumin (HSA) levels indicate a higher risk of acquiring and developing a severe C difficile infection (CDI) and are associated with recurrent and fatal disease.Methods: We used a combined approach based on docking simulation and biochemical analyses that were performed in vitro on purified proteins and in human epithelial colorectal adenocarcinoma cells (Caco-2), and in vivo on stem cell-derived human intestinal organoids and zebrafish embryos.Results: Our results show that HSA specifically binds via its domain II to TcdA and TcdB and thereby induces their autoproteolytic cleavage at physiological concentrations. This process impairs toxin internalization into the host cells and reduces the toxin-dependent glucosylation of Rho proteins.Conclusions: Our data provide evidence for a specific HSA-dependent self-defense mechanism against C difficile toxins and provide an explanation for the clinical correlation between CDI severity and hypoalbuminemia. [ABSTRACT FROM AUTHOR]

Published
2018
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41. Characterization of the cell penetrating properties of a human salivary proline-rich peptide

Author

Radicioni, Giorgia, primary, Stringaro, Annarita, additional, Molinari, Agnese, additional, Nocca, Giuseppina, additional, Longhi, Renato, additional, Pirolli, Davide, additional, Scarano, Emanuele, additional, Iavarone, Federica, additional, Manconi, Barbara, additional, Cabras, Tiziana, additional, Messana, Irene, additional, Castagnola, Massimo, additional, and Vitali, Alberto, additional

Published
2015
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43. NAC direct detoxification of TEGDMA cytotoxicity

Author

Spagnuolo, G, Virginia Rivieccio, V, Schweikl, H, Desiderio, Claudia, Lupi, Alessandro, Rengo, S, and Nocca, Giuseppina

Subjects
NAC, Methacrylates, Settore BIO/10 - BIOCHIMICA
Published
2013

46. Effects of ethanol and DMSO on TEGDMA solubility and cytotoxicity

Author

Rivieccio, V, Spagnuolo, G, Rengo, S, Lupi, Alessandro, and Nocca, Giuseppina

Subjects
solubility, TEGDMA, Settore BIO/10 - BIOCHIMICA
Abstract

Objective: Several in vitro studies have been carried out to investigate the triethylenglycol-dimethacrylate (TEGDMA) cytotoxicity. However, these studies have never focused on how the solvents used to dissolve TEGDMA in the experimental conditions might influence monomer effective concentrations and in turn its cytotoxicity. Hence, the aim of this study was to evaluate the active concentrations of the TEGDMA over the routinely experimental conditions used in biocompatibility in vitro tests and to determine any changes in cytotoxicity depending on the TEGDMA solution composition. Methods: TEGDMA dilutions were prepared directly in DMEM (in absence of cells) or were dissolved previously in DMSO or ethanol and then in medium. Monomer concentrations were quantified by an HPLC system. The cytotoxicity effects of TEGDMA dilutions (1 and 2 mmol/L, with and w/o solvents) were evaluated on 3T3-fibroblasts by MTT assay. ROS production (by FACScan flowcytometer) and intracellular and extracellular TEGDMA concentration (by HPLC) were also determined. Data were analyzed by ANOVA followed by Turkey's test for multiple comparisons. Results: Maximum solubility of TEGDMA in DMEM (in absence of cells) was 0.5 mmol/L both in the presence and absence of solvents. 2 mmol/L TEGDMA - solubilized in DMSO or ethanol and then dissolved in medium - caused a significant decrease in cell viability and an induction of ROS production compared to the same TEGDMA concentration dissolved in medium directly. Moreover when 2 mmol/L TEGDMA was added to the cells in presence of DMSO and ETOH, after 2h of incubation, TEGDMA concentration was reduced respectively 10% and 20%, while, TEGDMA added without vehicles remains constant. Conclusions: Our results showed that TEGDMA solubilization in DMEM was not complete and that the cytotoxic effects of the monomer was influenced by the method of solubilization.

Published
2011

47. In vitro evaluation of the cytotoxicity of FotoSan (TM) light-activated disinfection on human fibroblasts

Author

Gambarini, Gianluca, Plotino, Gianluca, Grande, Nicola M., Nocca, Giuseppina, Lupi, Alessandro, Giardina, Bruno, De Luca, Massimo, and Testarelli, Luca

Subjects
cytotoxicity, light-activated disinfection, root canal, Settore BIO/10 - BIOCHIMICA
Abstract

Background: Root canal disinfection needs to be improved because actual techniques are not able to eliminate all microorganisms present in the root canal system. The aim of the present study was to investigate the in vitro cytotoxicity of FotoSan (CMS Dental APS, Copenhagen Denmark), 17% EDTA and 2% chlorhexidine.

Published
2011

48. In vitro Evaluation of the Cytotoxicity of Different Root Canal Filling Materials

Author

Gambarini, G, Testarelli, L, Al Sudani, D, Plotino, Gianluca, Grande, Nicola Maria, Lupi, Alessandro, Giardina, Bruno, Nocca, Giuseppina, and de Luca, M.

Subjects
biocompatibility, filling materials, cytotoxicity, root canal, Settore BIO/10 - BIOCHIMICA, Article
Abstract

OBJECTIVE: Aim of the present study was to evaluate the cytotoxicity of Real Seal 1 compared to other commercially available endodontic filling materials: Real Seal (SybronEndo, Orange, CA, USA) and Thermafil (Tulsa Dental, Tulsa, OK, USA).,MATERIAL AND METHODS: Periodontal ligament cells from healthy patients were cultured. The eluate of Real Seal 1(TM) (RS1), Real Seal (RS) and Thermafil (TF) samples was used for the cells viability tests, both diluted (50%) or undiluted (100%). Incubation of the specimens was performed in culture medium for 24 h, 48 h and 72 h at 37 °C under sterile conditions. The cellular mortality was evaluated by MTT test. Results were statistically analysed and the statistical significance was set at p< 0.05.,RESULTS: None of the studied materials showed toxic effects during the period of observation (0 -72 h) when compared to the control group. Only RS induced a very modest increase in cell mortality (about 3% at both concentrations used, during the first 24 hours), when increasing the incubation time, however, only the lower concentration continued to show modest toxicity.,CONCLUSIONS: Results of the present study showed that all tested materials did not exhibit cytotoxic effects when compared to the control group.

Published
2011

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