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17 results on '"Nogueira, Paula M."'

1. Leishmania amazonensis from distinct clinical forms/hosts has polymorphisms in Lipophosphoglycans, displays variations in immunomodulatory properties and, susceptibility to antileishmanial drugs

Author

Rêgo, Felipe D., primary, Cardoso, Camila d. A., additional, Moreira, Paulo Otávio L., additional, Nogueira, Paula M., additional, Araújo, Márcio S., additional, Borges, Valéria Matos, additional, Laurenti, Márcia D., additional, Bartholomeu, Daniella C., additional, Reis, Alexandre B., additional, Monte‐Neto, Rubens L. d., additional, and Soares, Rodrigo P., additional

Published
2022
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2. Next-Generation Leishmanization: Revisiting Molecular Targets for Selecting Genetically Engineered Live-Attenuated Leishmania.

Author

Moreira, Paulo O. L., Nogueira, Paula M., and Monte-Neto, Rubens L.

Subjects
LEISHMANIA, DRUG target, GENOME editing, LEISHMANIASIS, VACCINE effectiveness, CRISPRS
Abstract

Despite decades of research devoted to finding a vaccine against leishmaniasis, we are still lacking a safe and effective vaccine for humans. Given this scenario, the search for a new prophylaxis alternative for controlling leishmaniasis should be a global priority. Inspired by leishmanization—a first generation vaccine strategy where live L. major parasites are inoculated in the skin to protect against reinfection—live-attenuated Leishmania vaccine candidates are promising alternatives due to their robust elicited protective immune response. In addition, they do not cause disease and could provide long-term protection upon challenge with a virulent strain. The discovery of a precise and easy way to perform CRISPR/Cas-based gene editing allowed the selection of safer null mutant live-attenuated Leishmania parasites obtained by gene disruption. Here, we revisited molecular targets associated with the selection of live-attenuated vaccinal strains, discussing their function, their limiting factors and the ideal candidate for the next generation of genetically engineered live-attenuated Leishmania vaccines to control leishmaniasis. [ABSTRACT FROM AUTHOR]

Published
2023
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8. Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome

Author

de Carvalho, Renan V.H., primary, Andrade, Warrison A., additional, Lima-Junior, Djalma S., additional, Dilucca, Marisa, additional, de Oliveira, Caroline V., additional, Wang, Kun, additional, Nogueira, Paula M., additional, Rugani, Jeronimo N., additional, Soares, Rodrigo P., additional, Beverley, Stephen M., additional, Shao, Feng, additional, and Zamboni, Dario S., additional

Published
2019
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10. Lipophosphoglycan polymorphisms do not affect Leishmania amazonensis development in the permissive vectors Lutzomyia migonei and Lutzomyia longipalpis

Author

Nogueira, Paula M., primary, Guimarães, Agna C., additional, Assis, Rafael R., additional, Sadlova, Jovana, additional, Myskova, Jitka, additional, Pruzinova, Katerina, additional, Hlavackova, Jana, additional, Turco, Salvatore J., additional, Torrecilhas, Ana C., additional, Volf, Petr, additional, and Soares, Rodrigo P., additional

Published
2017
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11. Lipophosphoglycans from Leishmania amazonensis Strains Display Immunomodulatory Properties via TLR4 and Do Not Affect Sand Fly Infection

Author

Nogueira, Paula M., primary, Assis, Rafael R., additional, Torrecilhas, Ana C., additional, Saraiva, Elvira M., additional, Pessoa, Natália L., additional, Campos, Marco A., additional, Marialva, Eric F., additional, Ríos-Velasquez, Cláudia M., additional, Pessoa, Felipe A., additional, Secundino, Nágila F., additional, Rugani, Jerônimo N., additional, Nieves, Elsa, additional, Turco, Salvatore J., additional, Melo, Maria N., additional, and Soares, Rodrigo P., additional

Published
2016
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14. Vesicles from differentTrypanosoma cruzistrains trigger differential innate and chronic immune responses

Author

Nogueira, Paula M., primary, Ribeiro, Kleber, additional, Silveira, Amanda C. O., additional, Campos, João H., additional, Martins-Filho, Olindo A., additional, Bela, Samantha R., additional, Campos, Marco A., additional, Pessoa, Natalia L., additional, Colli, Walter, additional, Alves, Maria J. M., additional, Soares, Rodrigo P., additional, and Torrecilhas, Ana Claudia, additional

Published
2015
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17. Vesicles from different Trypanosoma cruzi strains trigger differential innate and chronic immune responses.

Author

Nogueira, Paula M., Ribeiro, Kleber, Silveira, Amanda C. O., Campos, João H., Martins-Filho, Olindo A., Bela, Samantha R., Campos, Marco A., Pessoa, Natalia L., Colli, Walter, Alves, Maria J. M., Soares, Rodrigo P., and Torrecilhas, Ana Claudia

Abstract

Trypomastigote forms of Trypanosoma cruzi, the causative agent of Chagas Disease, shed extracellular vesicles (EVs) enriched with glycoproteins of the gp85/trans-sialidase (TS) superfamily and other α-galactosyl (α-Gal)-containing glycoconjugates, such as mucins. Here, purified vesicles from T. cruzi strains (Y, Colombiana, CL-14 and YuYu) were quantified according to size, intensity and concentration. Qualitative analysis revealed differences in their protein and α-galactosyl contents. Later, those polymorphisms were evaluated in the modulation of immune responses (innate and in the chronic phase) in C57BL/6 mice. EVs isolated from YuYu and CL-14 strains induced in macrophages higher levels of proinflammatory cytokines (TNF-α and IL-6) and nitric oxide via TLR2. In general, no differences were observed in MAPKs activation (p38, JNK and ERK 1/2) after EVs stimulation. In splenic cells derived from chronically infected mice, a different modulation pattern was observed, where Colombiana (followed by Y strain) EVs were more proinflammatory. This modulation was independent of the T. cruzi strain used in the mice infection. To test the functional importance of this modulation, the expression of intracellular cytokines after in vitro exposure was evaluated using EVs from YuYu and Colombiana strains. Both EVs induced cytokine production with the appearance of IL-10 in the chronically infected mice. A high frequency of IL-10 in CD4+ and CD8+ T lymphocytes was observed. A mixed profile of cytokine induction was observed in B cells with the production of TNF-α and IL-10. Finally, dendritic cells produced TNF-α after stimulation with EVs. Polymorphisms in the vesicles surface may be determinant in the immunopathologic events not only in the early steps of infection but also in the chronic phase. [ABSTRACT FROM AUTHOR]

Published
2015
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