50 results on '"Novelli, Flavia"'
Search Results
2. Apoptotic cell death in disease—Current understanding of the NCCD 2023
- Author
-
Vitale, Ilio, Pietrocola, Federico, Guilbaud, Emma, Aaronson, Stuart A., Abrams, John M., Adam, Dieter, Agostini, Massimiliano, Agostinis, Patrizia, Alnemri, Emad S., Altucci, Lucia, Amelio, Ivano, Andrews, David W., Aqeilan, Rami I., Arama, Eli, Baehrecke, Eric H., Balachandran, Siddharth, Bano, Daniele, Barlev, Nickolai A., Bartek, Jiri, Bazan, Nicolas G., Becker, Christoph, Bernassola, Francesca, Bertrand, Mathieu J. M., Bianchi, Marco E., Blagosklonny, Mikhail V., Blander, J. Magarian, Blandino, Giovanni, Blomgren, Klas, Borner, Christoph, Bortner, Carl D., Bove, Pierluigi, Boya, Patricia, Brenner, Catherine, Broz, Petr, Brunner, Thomas, Damgaard, Rune Busk, Calin, George A., Campanella, Michelangelo, Candi, Eleonora, Carbone, Michele, Carmona-Gutierrez, Didac, Cecconi, Francesco, Chan, Francis K. -M., Chen, Guo-Qiang, Chen, Quan, Chen, Youhai H., Cheng, Emily H., Chipuk, Jerry E., Cidlowski, John A., Ciechanover, Aaron, Ciliberto, Gennaro, Conrad, Marcus, Cubillos-Ruiz, Juan R., Czabotar, Peter E., D’Angiolella, Vincenzo, Daugaard, Mads, Dawson, Ted M., Dawson, Valina L., De Maria, Ruggero, De Strooper, Bart, Debatin, Klaus-Michael, Deberardinis, Ralph J., Degterev, Alexei, Del Sal, Giannino, Deshmukh, Mohanish, Di Virgilio, Francesco, Diederich, Marc, Dixon, Scott J., Dynlacht, Brian D., El-Deiry, Wafik S., Elrod, John W., Engeland, Kurt, Fimia, Gian Maria, Galassi, Claudia, Ganini, Carlo, Garcia-Saez, Ana J., Garg, Abhishek D., Garrido, Carmen, Gavathiotis, Evripidis, Gerlic, Motti, Ghosh, Sourav, Green, Douglas R., Greene, Lloyd A., Gronemeyer, Hinrich, Häcker, Georg, Hajnóczky, György, Hardwick, J. Marie, Haupt, Ygal, He, Sudan, Heery, David M., Hengartner, Michael O., Hetz, Claudio, Hildeman, David A., Ichijo, Hidenori, Inoue, Satoshi, Jäättelä, Marja, Janic, Ana, Joseph, Bertrand, Jost, Philipp J., Kanneganti, Thirumala-Devi, Karin, Michael, Kashkar, Hamid, Kaufmann, Thomas, Kelly, Gemma L., Kepp, Oliver, Kimchi, Adi, Kitsis, Richard N., Klionsky, Daniel J., Kluck, Ruth, Krysko, Dmitri V., Kulms, Dagmar, Kumar, Sharad, Lavandero, Sergio, Lavrik, Inna N., Lemasters, John J., Liccardi, Gianmaria, Linkermann, Andreas, Lipton, Stuart A., Lockshin, Richard A., López-Otín, Carlos, Luedde, Tom, MacFarlane, Marion, Madeo, Frank, Malorni, Walter, Manic, Gwenola, Mantovani, Roberto, Marchi, Saverio, Marine, Jean-Christophe, Martin, Seamus J., Martinou, Jean-Claude, Mastroberardino, Pier G., Medema, Jan Paul, Mehlen, Patrick, Meier, Pascal, Melino, Gerry, Melino, Sonia, Miao, Edward A., Moll, Ute M., Muñoz-Pinedo, Cristina, Murphy, Daniel J., Niklison-Chirou, Maria Victoria, Novelli, Flavia, Núñez, Gabriel, Oberst, Andrew, Ofengeim, Dimitry, Opferman, Joseph T., Oren, Moshe, Pagano, Michele, Panaretakis, Theocharis, Pasparakis, Manolis, Penninger, Josef M., Pentimalli, Francesca, Pereira, David M., Pervaiz, Shazib, Peter, Marcus E., Pinton, Paolo, Porta, Giovanni, Prehn, Jochen H. M., Puthalakath, Hamsa, Rabinovich, Gabriel A., Rajalingam, Krishnaraj, Ravichandran, Kodi S., Rehm, Markus, Ricci, Jean-Ehrland, Rizzuto, Rosario, Robinson, Nirmal, Rodrigues, Cecilia M. P., Rotblat, Barak, Rothlin, Carla V., Rubinsztein, David C., Rudel, Thomas, Rufini, Alessandro, Ryan, Kevin M., Sarosiek, Kristopher A., Sawa, Akira, Sayan, Emre, Schroder, Kate, Scorrano, Luca, Sesti, Federico, Shao, Feng, Shi, Yufang, Sica, Giuseppe S., Silke, John, Simon, Hans-Uwe, Sistigu, Antonella, Stephanou, Anastasis, Stockwell, Brent R., Strapazzon, Flavie, Strasser, Andreas, Sun, Liming, Sun, Erwei, Sun, Qiang, Szabadkai, Gyorgy, Tait, Stephen W. G., Tang, Daolin, Tavernarakis, Nektarios, Troy, Carol M., Turk, Boris, Urbano, Nicoletta, Vandenabeele, Peter, Vanden Berghe, Tom, Vander Heiden, Matthew G., Vanderluit, Jacqueline L., Verkhratsky, Alexei, Villunger, Andreas, von Karstedt, Silvia, Voss, Anne K., Vousden, Karen H., Vucic, Domagoj, Vuri, Daniela, Wagner, Erwin F., Walczak, Henning, Wallach, David, Wang, Ruoning, Wang, Ying, Weber, Achim, Wood, Will, Yamazaki, Takahiro, Yang, Huang-Tian, Zakeri, Zahra, Zawacka-Pankau, Joanna E., Zhang, Lin, Zhang, Haibing, Zhivotovsky, Boris, Zhou, Wenzhao, Piacentini, Mauro, Kroemer, Guido, and Galluzzi, Lorenzo
- Published
- 2023
- Full Text
- View/download PDF
3. Tumour predisposition and cancer syndromes as models to study gene-environment interactions.
- Author
-
Carbone, Michele, Arron, Sarah T, Beutler, Bruce, Bononi, Angela, Cavenee, Webster, Cleaver, James E, Croce, Carlo M, D'Andrea, Alan, Foulkes, William D, Gaudino, Giovanni, Groden, Joanna L, Henske, Elizabeth P, Hickson, Ian D, Hwang, Paul M, Kolodner, Richard D, Mak, Tak W, Malkin, David, Monnat, Raymond J, Novelli, Flavia, Pass, Harvey I, Petrini, John H, Schmidt, Laura S, and Yang, Haining
- Subjects
Animals ,Humans ,Neoplasms ,Genetic Predisposition to Disease ,Germ-Line Mutation ,Gene-Environment Interaction ,Genetics ,Cancer ,Prevention ,2.2 Factors relating to the physical environment ,2.1 Biological and endogenous factors ,Aetiology ,Medical and Health Sciences ,Oncology & Carcinogenesis - Abstract
Cell division and organismal development are exquisitely orchestrated and regulated processes. The dysregulation of the molecular mechanisms underlying these processes may cause cancer, a consequence of cell-intrinsic and/or cell-extrinsic events. Cellular DNA can be damaged by spontaneous hydrolysis, reactive oxygen species, aberrant cellular metabolism or other perturbations that cause DNA damage. Moreover, several environmental factors may damage the DNA, alter cellular metabolism or affect the ability of cells to interact with their microenvironment. While some environmental factors are well established as carcinogens, there remains a large knowledge gap of others owing to the difficulty in identifying them because of the typically long interval between carcinogen exposure and cancer diagnosis. DNA damage increases in cells harbouring mutations that impair their ability to correctly repair the DNA. Tumour predisposition syndromes in which cancers arise at an accelerated rate and in different organs - the equivalent of a sensitized background - provide a unique opportunity to examine how gene-environment interactions influence cancer risk when the initiating genetic defect responsible for malignancy is known. Understanding the molecular processes that are altered by specific germline mutations, environmental exposures and related mechanisms that promote cancer will allow the design of novel and effective preventive and therapeutic strategies.
- Published
- 2020
4. BAP1 forms a trimer with HMGB1 and HDAC1 that modulates gene × environment interaction with asbestos
- Author
-
Novelli, Flavia, Bononi, Angela, Wang, Qian, Bai, Fang, Patergnani, Simone, Kricek, Franz, Haglund, Ellinor, Suarez, Joelle S., Tanji, Mika, Xu, Ronghui, Takanishi, Yasutaka, Minaai, Michael, Pastorino, Sandra, Morris, Paul, Sakamoto, Greg, Pass, Harvey I., Barbour, Haithem, Gaudino, Giovanni, Giorgi, Carlotta, Pinton, Paolo, Onuchic, Jose N., Yang, Haining, and Carbone, Michele
- Published
- 2021
5. Heterozygous germline BLM mutations increase susceptibility to asbestos and mesothelioma
- Author
-
Bononi, Angela, Goto, Keisuke, Ak, Guntulu, Yoshikawa, Yoshie, Emi, Mitsuru, Pastorino, Sandra, Carparelli, Lorenzo, Ferro, Angelica, Nasu, Masaki, Kim, Jin-Hee, Suarez, Joelle S., Xu, Ronghui, Tanji, Mika, Takinishi, Yasutaka, Minaai, Michael, Novelli, Flavia, Pagano, Ian, Gaudino, Giovanni, Pass, Harvey I., Groden, Joanna, Grzymski, Joseph J., Metintas, Muzaffer, Akarsu, Muhittin, Morrow, Betsy, Hassan, Raffit, Yang, Haining, and Carbone, Michele
- Published
- 2020
6. Asbestos induces mesothelial cell transformation via HMGB1-driven autophagy
- Author
-
Xue, Jiaming, Patergnani, Simone, Giorgi, Carlotta, Suarez, Joelle, Goto, Keisuke, Bononi, Angela, Tanji, Mika, Novelli, Flavia, Pastorino, Sandra, Xu, Ronghui, Caroccia, Natascia, Dogan, A. Umran, Pass, Harvey I., Tognon, Mauro, Pinton, Paolo, Gaudino, Giovanni, Mak, Tak W., Carbone, Michele, and Yang, Haining
- Published
- 2020
7. Germline BARD1 variants predispose to mesothelioma by impairing DNA repair and calcium signaling.
- Author
-
Novelli, Flavia, Yoshikawa, Yoshie, Maria Vitto, Veronica Angela, Modesti, Lorenzo, Minaai, Michael, Pastorino, Sandra, Mitsuru Emi, Jin-Hee Kim, Kricek, Franz, Fang Bai, Onuchic, José N., Bononi, Angela, Suarez, Joelle S., Tanji, Mika, Favaron, Cristina, Zolondick, Alicia A., Ronghui Xu, Yasutaka Takanishi, Zhanwei Wang, and Sakamoto, Greg
- Subjects
- *
P53 protein , *DNA repair , *NATURE & nurture , *MISSENSE mutation , *ECOLOGICAL genetics - Abstract
We report that ~1.8% of all mesothelioma patients and 4.9% of those younger than 55, carry rare germline variants of the BRCA1 associated RING domain 1 (BARD1) gene that were predicted to be damaging by computational analyses. We conducted functional assays, essential for accurate interpretation of missense variants, in primary fibroblasts that we established in tissue culture from a patient carrying the heterozygous BARD1V523A mutation. We found that these cells had genomic instability, reduced DNA repair, and impaired apoptosis. Investigating the underlying signaling pathways, we found that BARD1 forms a trimeric protein complex with p53 and SERCA2 that regulates calcium signaling and apoptosis. We validated these findings in BARD1-silenced primary human mesothelial cells exposed to asbestos. Our study elucidated mechanisms of BARD1 activity and revealed that heterozygous germline BARD1 mutations favor the development of mesothelioma and increase the susceptibility to asbestos carcinogenesis. These mesotheliomas are significantly less aggressive compared to mesotheliomas in asbestos workers. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
8. Validation of a biomarker tool capable of measuring the absorbed dose soon after exposure to ionizing radiation
- Author
-
Giovanetti, Anna, Marconi, Raffaella, Awad, Noha, Abuzied, Hala, Agamy, Neveen, Barakat, Mohamed, Bartoleschi, Cecilia, Bossi, Gianluca, Canfora, Marco, Elsaid, Amr A., Ioannilli, Laura, Ismail, Horeya M., Issa, Yasmine Amr, Novelli, Flavia, Pardini, Maria Chiara, Pioli, Claudio, Pinnarò, Paola, Sanguineti, Giuseppe, Tahoun, Mohamed M., Turchi, Riccardo, and Strigari, Lidia
- Published
- 2021
- Full Text
- View/download PDF
9. Apoptotic cell death in disease—Current understanding of the NCCD 2023
- Author
-
Associazione Italiana per la Ricerca sul Cancro, Italian Institute for Genomic Medicine, Compagnia di San Paolo, Vitale, Ilio [0000-0002-5918-1841], Pietrocola, Federico [0000-0002-2930-234X], Guilbaud, Emma [0000-0001-5261-1944], Aaronson, Stuart A. [0000-0002-4643-0474], Dieter, Adam [0000-0002-5668-5032], Agostini, Massimiliano [0000-0003-3124-2072], Agostinis, Patrizia [0000-0003-1314-2115], Alnemri, Emad S. [0000-0002-7295-3383], Altucci, Lucia [0000-0002-7312-5387], Amelio, Ivano [0000-0002-9126-5391], Andrews, David W. [0000-0002-9266-7157], Aqeilan, Rami I. [0000-0002-6034-023X], Arama, Eli [0000-0001-5953-0629], Balachandran, Siddharth [0000-0003-2084-1803], Bano, Daniele [0000-0002-9617-5504], Bartek, Jiri [0000-0003-2013-7525], Bazan, Nicolas G. [0000-0002-9243-5444], Bernassola, Francesca [0000-0002-8883-8654], Bertrand, Mathieu J. M. [0000-0001-9000-0626], Bianchi, Marco Emilio [0000-0002-5329-6445], Blander, J. Magarian [0000-0001-9207-1700], Blandino, Giovanni [0000-0002-6970-2241], Blomgren, Klas [0000-0002-0476-7271], Bortner, Carl D. [0000-0002-5444-6628], Bove, Pierluigi [0000-0002-4788-2982], Boya, Patricia [0000-0003-3045-951X], Broz, Petr [0000-0002-2334-7790], Damgaard, Rune Busk [0000-0002-1709-6534], Calin, George A. [0000-0002-7427-0578], Campanella, Michelangelo [0000-0002-6948-4184], Candi, Eleonora [0000-0001-8332-4825], Carbone, Michele [0000-0001-8928-8474], Carmona-Gutierrez, Didac [0000-0001-7548-7771], Cecconi, Francesco [0000-0002-5614-4359], Chen, Guo‑Qiang [0000-0002-7226-1782], Cheng, Emily H. [0000-0002-3595-2648], Chipuk, Jerry E. [0000-0002-1337-842X], Cidlowski, John A. [0000-0003-1420-0516], Ciechanover, Aaron [0000-0001-9184-8944], Ciliberto, Gennaro [0000-0003-2851-8605], Conrad, Marcus [0000-0003-1140-5612], Czabotar, Peter E. [0000-0002-2594-496X], D’Angiolella, Vincenzo [0000-0001-8365-9094], Daugaard, Mads [0000-0001-8383-055X], Dawson, Valina L. [0000-0002-2915-3970], De Maria, Ruggero [0000-0003-2255-0583], Debatin, Klaus-Michael [0000-0002-8397-1886], Deberardinis, Ralph J. [0000-0002-2705-7432], Degterev, Alexei [0000-0002-8240-7132], Del Sal, Giannino [0000-0003-2185-6003], Deshmukh, Mohanish [0000-0002-2597-5862], Di Virgilio, Francesco [0000-0003-3566-1362], Diederich, Marc [0000-0003-0115-4725], Dixon, Scott J. [0000-0001-6230-8199], El-Deiry, Wafik S. [0000-0002-9577-8266], Elrod, John W. [0000-0003-3925-2224], Engeland, Kurt [0000-0003-3525-0440], Fimia, Gian María [0000-0003-4438-3325], Ganini, Carlo [0000-0002-5839-3965], García-Sáez, Ana J. [0000-0002-3894-5945], Garg, Abhishek D. [0000-0002-9976-9922], Garrido, Carmen [0000-0003-1368-1493], Gavathiotis, Evripidis [0000-0001-6319-8331], Ghosh, Sourav [0000-0001-5990-8708], Green, Douglas R. [0000-0002-7332-1417], Gronemeyer, Hinrich [0000-0001-9454-2449}, Häcker, Georg [0000-0003-1058-5746], Hajnóczky, György [0000-0003-3813-2570], Hardwick, J. Marie [0000-0002-4847-2045], Haupt, Ygal [0000-0001-5925-0096], He, Sudan [0000-0002-0846-1210], Heery, David M. [0000-0002-5035-2392], Hengartner, Michael O. [0000-0002-7584-596X], Hetz, Claudio [0000-0003-1120-7966], Hildeman, David A. [0000-0002-0421-8483], Ichijo, Hidenori [0000-0002-5005-6438], Jäättelä, Marja [0000-0001-5950-7111], Janic, Ana [0000-0002-4200-2560], Joseph, Bertrand [0000-0001-5655-9979], Jost, Philipp J. [0000-0003-2454-0362], Kanneganti, Thirumala-Devi [0000-0002-6395-6443], Karin, Michael [0000-0002-2758-6473], Kashkar, Hamid [0000-0003-2796-1429], Kaufmann, Thomas [0000-0001-9906-874X], Kelly, Gemma L. [0000-0002-6533-1201], Kepp, Oliver [0000-0002-6081-9558], Kimchi, Adi [0000-0002-8236-8989], Klionsky, Daniel J. [0000-0002-7828-8118], Kluck, Ruth [0000-0002-7101-1925], Krysko, Dmitri V. [0000-0002-9692-2047], Kulms, Dagmar [0000-0001-6874-0548], Kumar, Sharad [0000-0001-7126-9814], Lavandero, Sergio [0000-0003-4258-1483], Lavrik, Inna N. [0000-0002-9324-309X], Liccardi, Gianmaria [0000-0002-2662-1281], Linkermann, Andreas [0000-0001-6287-9725], Lipton, Stuart A. [0000-0002-3490-1259], Lockshin, Richard A. [0000-0002-4389-4898], López-Otín, Carlos [0000-0001-6964-1904], Luedde, Tom [0000-0002-6288-8821], MacFarlane, Marion [0000-0001-7886-1159], Madeo, Frank [0000-0002-5070-1329], Malorni, Walter [0000-0002-1223-7000], Manic, Gwenola [0000-0003-3759-8029], Marchi, Saverio [0000-0003-2708-1843], Marine, Jean-Christophe [0000-0003-2433-9837], Martin, Seamus J. [0000-0002-8539-3143], Martinou, Jean-Claude [0000-0002-9847-2051], Mastroberardino, Pier G. [0000-0003-2364-4258], Medema, Jan Paul [0000-0003-3045-2924], Mehlen, Patrick [0000-0003-1743-5417], Meier, Pascal [0000-0003-2760-6523], Melino, Gerry [0000-0001-9428-5972], Melino, Sonia [0000-0001-7694-5279], Miao, Edward A. [0000-0001-7295-3490], Moll, Ute M. [0000-0003-1908-7516], Muñoz-Pinedo, Cristina [0000-0002-9120-664X], Murphy, Daniel J. [0000-0002-5538-5468], Niklison-Chirou, Maria Victoria [0000-0002-2147-370X], Novelli, Flavia [0000-0002-3746-7478], Oberst, Andrew [0000-0002-9500-7912], Ofengeim, Dimitry [0000-0003-2348-3642], Opferman, Joseph T. [0000-0002-1147-5621], Oren, Moshe [0000-0003-4311-7172], Pagano, Michele [0000-0003-3210-2442], Panaretakis, Theocharis [0000-0001-5754-6950], Pasparakis, Manolis [0000-0002-9870-0966], Penninger, Josef M. [0000-0002-8194-3777], Pentimalli, Francesca [0000-0003-4740-6801], Pereira, David M. [0000-0003-0384-7592], Pervaiz, Shazib [0000-0002-4738-019X], Peter, Marcus E. [0000-0003-3216-036X], Pinton, Paolo [0000-0001-7108-6508], Porta, Giovanni [0000-0001-5260-2415], Puthalakath, Hamsa [0000-0001-5178-1175], Rabinovich, Gabriel A. [0000-0002-0947-8735], Rajalingam, Krishnaraj [0000-0002-4175-9633], Ravinchandran, Kodi S. [0000-0001-9049-1410], Rehm, Markus [0000-0001-6149-9261], Ricci, Jean-Ehrland [0000-0003-1585-8117], Rizzuto, Rosario [0000-0001-7044-5097], Robinson, Nirmal [0000-0002-7361-9491], Rotblat, Barak [0000-0003-2985-7115], Rothlin, Carla V. [0000-0002-5693-5572], Rubinsztein, David C. [0000-0001-5002-5263], Rufini, Alessandro [0000-0002-5855-655X], Ryan, Kevin M. [0000-0002-1059-9681], Sarosiek, Kristopher A. [0000-0002-4618-5085], Sawa, Akira [0000-0003-1401-3008], Sayan, Emre [0000-0002-5291-1485], Schroder, Kate [0000-0001-9261-3805], Scorrano, Luca [0000-0002-8515-8928], Sesti, Federico [0000-0002-2761-9693], Shi, Yufang [0000-0001-8964-319X], Sica, Giuseppe [0000-0002-7407-0584], Silke, John [0000-0002-7611-5774], Simon, Hans-Uwe [0000-0002-9404-7736], Sistigu, Antonella [0000-0002-2528-1238], Stockwell, Brent R. [0000-0002-3532-3868], Strappazzon, Flavie [0000-0003-0285-7449], Sun, Liming [0000-0002-0136-5605], Sun, Erwei [0000-0001-5664-513X], Szabadkai, G [0000-0002-3006-3577], Tait, Stephen W. G. [0000-0001-7697-132X], Tang, Daolin [0000-0002-1903-6180], Tavernarakis, Nektarios [0000-0002-5253-1466], Turk, Boris [0000-0002-9007-5764], Urbano, Nicoletta [0000-0003-1822-155X], Vandenabeele, Peter [0000-0002-6669-8822], Vanden Berghe, Tom [0000-0002-1633-0974], Vander Heiden, Matthew G. [0000-0002-6702-4192], Vanderluit, Jacqueline L. [0000-0002-4960-920X], Verkhratsky, A. [0000-0003-2592-9898], Villunger, Andreas [0000-0001-8259-4153], Von Karstedt, Silvia [0000-0002-7816-5919], Voss, Anne K. [0000-0002-3853-9381], Vucic, Domagoj [0000-0003-3614-8093], Vuri, Daniela [0000-0001-8693-3845], Wagner, Erwin F. [0000-0001-7872-0196], Walczak, Henning [0000-0002-6312-4591], Wallach, David [0000-0003-2724-9757], Wang, Ruoning [0000-0001-9798-8032], Weber, Achim [0000-0003-0073-3637], Yamazaki, Takahiro [0000-0002-7420-4394], Zakeri, Zahra [0000-0003-4386-8072], Zawacka-Pankau, Joanna E. [0000-0002-7415-2942], Zhivotovsky, Boris [0000-0002-2238-3482], Piacentini, Mauro [0000-0003-2919-1296], Kroemer, Guido [0000-0002-9334-4405], Galluzzi, Lorenzo [0000-0003-2257-8500 ], Vitale, Ilio, Pietrocola, Federico, Guilbaud, Emma, Aaronson, Stuart A., Abrams, John M., Dieter, Adam, Agostini, Massimiliano, Agostinis, Patrizia, Alnemri, Emad S., Altucci, Lucia, Amelio, Ivano, Andrews, David W., Aqeilan, Rami I., Arama, Eli, Baehrecke, Eric H., Balachandran, Siddharth, Bano, Daniele, Barlev, Nickolai A., Bartek, Jiri, Bazan, Nicolas G., Becker, Christoph, Bernassola, Francesca, Bertrand, Mathieu J. M., Bianchi, Marco Emilio, Blagosklonny, Mikhail V., Blander, J. Magarian, Blandino, Giovanni, Blomgren, Klas, Bomer, Christoph, Bortner, Carl D., Bove, Pierluigi, Boya, Patricia, Brenner, Catherine, Broz, Petr, Brunner, T., Damgaard, Rune Busk, Calin, George A., Campanella, Michelangelo, Candi, Eleonora, Carbone, Michele, Carmona-Gutierrez, Didac, Cecconi, Francesco, Chan, Francis K.-M., Chen, Guo‑Qiang, Chen, Quan, Chen, Youhai H., Cheng, Emily H., Chipuk, Jerry E., Cidlowski, John A., Ciechanover, Aaron, Ciliberto, Gennaro, Conrad, Marcus, Cubillos-Ruiz, Juan R., Czabotar, Peter E., D’Angiolella, Vincenzo, Daugaard, Mads, Dawson, Ted M., Dawson, Valina L., De Maria, Ruggero, De Strooper, B., Debatin, Klaus-Michael, Deberardinis, Ralph J., Degterev, Alexei, Del Sal, Giannino, Deshmukh, Mohanish, Di Virgilio, Francesco, Diederich, Marc, Dixon, Scott J., Dynlacht, Brian D., El-Deiry, Wafik S., Elrod, John W., Engeland, Kurt, Fimia, Gian María, Galassi, Claudia, Ganini, Carlo, García-Sáez, Ana J., Garg, Abhishek D., Garrido, Carmen, Gavathiotis, Evripidis, Gerlic, Motti, Ghosh, Sourav, Green, Douglas R., Greene, Lloyd A., Gronemeyer, Hinrich, Häcker, Georg, Hajnóczky, György, Hardwick, J. Marie, Haupt, Ygal, He, Sudan, Heery, David M., Hengartner, Michael O., Hetz, Claudio, Hildeman, David A., Ichijo, Hidenori, Inoue, Satoshi, Jäättelä, Marja, Janic, Ana, Joseph, Bertrand, Jost, Philipp J., Kanneganti, Thirumala-Devi, Karin, Michael, Kashkar, Hamid, Kaufmann, Thomas, Kelly, Gemma L., Kepp, Oliver, Kimchi, Adi, Kitsis, Richard N., Klionsky, Daniel J., Kluck, Ruth, Krysko, Dmitri V., Kulms, Dagmar, Kumar, Sharad, Lavandero, Sergio, Lavrik, Inna N., Lemasters, John J., Liccardi, Gianmaria, Linkermann, Andreas, Lipton, Stuart A., Lockshin, Richard A., López-Otín, Carlos, Luedde, Tom, MacFarlane, Marion, Madeo, Frank, Malorni, Walter, Manic, Gwenola, Mantovani, Roberto, Marchi, Saverio, Marine, Jean-Christophe, Martin, Seamus J., Martinou, Jean-Claude, Mastroberardino, Pier G., Medema, Jan Paul, Mehlen, Patrick, Meier, Pascal, Melino, Gerry, Melino, Sonia, Miao, Edward A., Moll, Ute M., Muñoz-Pinedo, Cristina, Murphy, Daniel J., Niklison-Chirou, Maria Victoria, Novelli, Flavia, Núñez, Gabriel, Oberst, Andrew, Ofengeim, Dimitry, Opferman, Joseph T., Oren, Moshe, Pagano, Michele, Panaretakis, Theocharis, Pasparakis, Manolis, Penninger, Josef M., Pentimalli, Francesca, Pereira, David M., Pervaiz, Shazib, Peter, Marcus E., Pinton, Paolo, Porta, Giovanni, Prehn, Jochen H. M., Puthalakath, Hamsa, Rabinovich, Gabriel A., Rajalingam, Krishnaraj, Ravinchandran, Kodi S., Rehm, Markus, Ricci, Jean-Ehrland, Rizzuto, Rosario, Robinson, Nirmal, Rodrigues, Cecilia M. P., Rotblat, Barak, Rothlin, Carla V., Rubinsztein, David C., Rudel, Thomas, Rufini, Alessandro, Ryan, Kevin M., Sarosiek, Kristopher A., Sawa, Akira, Sayan, Emre, Schroder, Kate, Scorrano, Luca, Sesti, Federico, Shao, Feng, Shi, Yufang, Sica, Giuseppe, Silke, John, Simon, Hans-Uwe, Sistigu, Antonella, Stephanou, Anastasis, Stockwell, Brent R., Strappazzon, Flavie, Strasser, Andreas, Sun, Liming, Sun, Erwei, Sun, Qiang, Szabadkai, G, Tait, Stephen W. G., Tang, Daolin, Tavernarakis, Nektarios, Troy, Carol M., Turk, Boris, Urbano, Nicoletta, Vandenabeele, Peter, Vanden Berghe, Tom, Vander Heiden, Matthew G., Vanderluit, Jacqueline L., Verkhratsky, A., Villunger, Andreas, Von Karstedt, Silvia, Voss, Anne K., Vousden, Karen H., Vucic, Domagoj, Vuri, Daniela, Wagner, Erwin F., Walczak, Henning, Wallach, David, Wang, Ruoning, Wang, Ying, Weber, Achim, Wood, Will, Yamazaki, Takahiro, Yang, Zahra, Zakeri, Zahra, Zawacka-Pankau, Joanna E., Zhang, Lin, Zhang, Haibin, Zhivotovsky, Boris, Zhou, Wenzhao, Piacentini, Mauro, Kroemer, Guido, Galluzzi, Lorenzo, Associazione Italiana per la Ricerca sul Cancro, Italian Institute for Genomic Medicine, Compagnia di San Paolo, Vitale, Ilio [0000-0002-5918-1841], Pietrocola, Federico [0000-0002-2930-234X], Guilbaud, Emma [0000-0001-5261-1944], Aaronson, Stuart A. [0000-0002-4643-0474], Dieter, Adam [0000-0002-5668-5032], Agostini, Massimiliano [0000-0003-3124-2072], Agostinis, Patrizia [0000-0003-1314-2115], Alnemri, Emad S. [0000-0002-7295-3383], Altucci, Lucia [0000-0002-7312-5387], Amelio, Ivano [0000-0002-9126-5391], Andrews, David W. [0000-0002-9266-7157], Aqeilan, Rami I. [0000-0002-6034-023X], Arama, Eli [0000-0001-5953-0629], Balachandran, Siddharth [0000-0003-2084-1803], Bano, Daniele [0000-0002-9617-5504], Bartek, Jiri [0000-0003-2013-7525], Bazan, Nicolas G. [0000-0002-9243-5444], Bernassola, Francesca [0000-0002-8883-8654], Bertrand, Mathieu J. M. [0000-0001-9000-0626], Bianchi, Marco Emilio [0000-0002-5329-6445], Blander, J. Magarian [0000-0001-9207-1700], Blandino, Giovanni [0000-0002-6970-2241], Blomgren, Klas [0000-0002-0476-7271], Bortner, Carl D. [0000-0002-5444-6628], Bove, Pierluigi [0000-0002-4788-2982], Boya, Patricia [0000-0003-3045-951X], Broz, Petr [0000-0002-2334-7790], Damgaard, Rune Busk [0000-0002-1709-6534], Calin, George A. [0000-0002-7427-0578], Campanella, Michelangelo [0000-0002-6948-4184], Candi, Eleonora [0000-0001-8332-4825], Carbone, Michele [0000-0001-8928-8474], Carmona-Gutierrez, Didac [0000-0001-7548-7771], Cecconi, Francesco [0000-0002-5614-4359], Chen, Guo‑Qiang [0000-0002-7226-1782], Cheng, Emily H. [0000-0002-3595-2648], Chipuk, Jerry E. [0000-0002-1337-842X], Cidlowski, John A. [0000-0003-1420-0516], Ciechanover, Aaron [0000-0001-9184-8944], Ciliberto, Gennaro [0000-0003-2851-8605], Conrad, Marcus [0000-0003-1140-5612], Czabotar, Peter E. [0000-0002-2594-496X], D’Angiolella, Vincenzo [0000-0001-8365-9094], Daugaard, Mads [0000-0001-8383-055X], Dawson, Valina L. [0000-0002-2915-3970], De Maria, Ruggero [0000-0003-2255-0583], Debatin, Klaus-Michael [0000-0002-8397-1886], Deberardinis, Ralph J. [0000-0002-2705-7432], Degterev, Alexei [0000-0002-8240-7132], Del Sal, Giannino [0000-0003-2185-6003], Deshmukh, Mohanish [0000-0002-2597-5862], Di Virgilio, Francesco [0000-0003-3566-1362], Diederich, Marc [0000-0003-0115-4725], Dixon, Scott J. [0000-0001-6230-8199], El-Deiry, Wafik S. [0000-0002-9577-8266], Elrod, John W. [0000-0003-3925-2224], Engeland, Kurt [0000-0003-3525-0440], Fimia, Gian María [0000-0003-4438-3325], Ganini, Carlo [0000-0002-5839-3965], García-Sáez, Ana J. [0000-0002-3894-5945], Garg, Abhishek D. [0000-0002-9976-9922], Garrido, Carmen [0000-0003-1368-1493], Gavathiotis, Evripidis [0000-0001-6319-8331], Ghosh, Sourav [0000-0001-5990-8708], Green, Douglas R. [0000-0002-7332-1417], Gronemeyer, Hinrich [0000-0001-9454-2449}, Häcker, Georg [0000-0003-1058-5746], Hajnóczky, György [0000-0003-3813-2570], Hardwick, J. Marie [0000-0002-4847-2045], Haupt, Ygal [0000-0001-5925-0096], He, Sudan [0000-0002-0846-1210], Heery, David M. [0000-0002-5035-2392], Hengartner, Michael O. [0000-0002-7584-596X], Hetz, Claudio [0000-0003-1120-7966], Hildeman, David A. [0000-0002-0421-8483], Ichijo, Hidenori [0000-0002-5005-6438], Jäättelä, Marja [0000-0001-5950-7111], Janic, Ana [0000-0002-4200-2560], Joseph, Bertrand [0000-0001-5655-9979], Jost, Philipp J. [0000-0003-2454-0362], Kanneganti, Thirumala-Devi [0000-0002-6395-6443], Karin, Michael [0000-0002-2758-6473], Kashkar, Hamid [0000-0003-2796-1429], Kaufmann, Thomas [0000-0001-9906-874X], Kelly, Gemma L. [0000-0002-6533-1201], Kepp, Oliver [0000-0002-6081-9558], Kimchi, Adi [0000-0002-8236-8989], Klionsky, Daniel J. [0000-0002-7828-8118], Kluck, Ruth [0000-0002-7101-1925], Krysko, Dmitri V. [0000-0002-9692-2047], Kulms, Dagmar [0000-0001-6874-0548], Kumar, Sharad [0000-0001-7126-9814], Lavandero, Sergio [0000-0003-4258-1483], Lavrik, Inna N. [0000-0002-9324-309X], Liccardi, Gianmaria [0000-0002-2662-1281], Linkermann, Andreas [0000-0001-6287-9725], Lipton, Stuart A. [0000-0002-3490-1259], Lockshin, Richard A. [0000-0002-4389-4898], López-Otín, Carlos [0000-0001-6964-1904], Luedde, Tom [0000-0002-6288-8821], MacFarlane, Marion [0000-0001-7886-1159], Madeo, Frank [0000-0002-5070-1329], Malorni, Walter [0000-0002-1223-7000], Manic, Gwenola [0000-0003-3759-8029], Marchi, Saverio [0000-0003-2708-1843], Marine, Jean-Christophe [0000-0003-2433-9837], Martin, Seamus J. [0000-0002-8539-3143], Martinou, Jean-Claude [0000-0002-9847-2051], Mastroberardino, Pier G. [0000-0003-2364-4258], Medema, Jan Paul [0000-0003-3045-2924], Mehlen, Patrick [0000-0003-1743-5417], Meier, Pascal [0000-0003-2760-6523], Melino, Gerry [0000-0001-9428-5972], Melino, Sonia [0000-0001-7694-5279], Miao, Edward A. [0000-0001-7295-3490], Moll, Ute M. [0000-0003-1908-7516], Muñoz-Pinedo, Cristina [0000-0002-9120-664X], Murphy, Daniel J. [0000-0002-5538-5468], Niklison-Chirou, Maria Victoria [0000-0002-2147-370X], Novelli, Flavia [0000-0002-3746-7478], Oberst, Andrew [0000-0002-9500-7912], Ofengeim, Dimitry [0000-0003-2348-3642], Opferman, Joseph T. [0000-0002-1147-5621], Oren, Moshe [0000-0003-4311-7172], Pagano, Michele [0000-0003-3210-2442], Panaretakis, Theocharis [0000-0001-5754-6950], Pasparakis, Manolis [0000-0002-9870-0966], Penninger, Josef M. [0000-0002-8194-3777], Pentimalli, Francesca [0000-0003-4740-6801], Pereira, David M. [0000-0003-0384-7592], Pervaiz, Shazib [0000-0002-4738-019X], Peter, Marcus E. [0000-0003-3216-036X], Pinton, Paolo [0000-0001-7108-6508], Porta, Giovanni [0000-0001-5260-2415], Puthalakath, Hamsa [0000-0001-5178-1175], Rabinovich, Gabriel A. [0000-0002-0947-8735], Rajalingam, Krishnaraj [0000-0002-4175-9633], Ravinchandran, Kodi S. [0000-0001-9049-1410], Rehm, Markus [0000-0001-6149-9261], Ricci, Jean-Ehrland [0000-0003-1585-8117], Rizzuto, Rosario [0000-0001-7044-5097], Robinson, Nirmal [0000-0002-7361-9491], Rotblat, Barak [0000-0003-2985-7115], Rothlin, Carla V. [0000-0002-5693-5572], Rubinsztein, David C. [0000-0001-5002-5263], Rufini, Alessandro [0000-0002-5855-655X], Ryan, Kevin M. [0000-0002-1059-9681], Sarosiek, Kristopher A. [0000-0002-4618-5085], Sawa, Akira [0000-0003-1401-3008], Sayan, Emre [0000-0002-5291-1485], Schroder, Kate [0000-0001-9261-3805], Scorrano, Luca [0000-0002-8515-8928], Sesti, Federico [0000-0002-2761-9693], Shi, Yufang [0000-0001-8964-319X], Sica, Giuseppe [0000-0002-7407-0584], Silke, John [0000-0002-7611-5774], Simon, Hans-Uwe [0000-0002-9404-7736], Sistigu, Antonella [0000-0002-2528-1238], Stockwell, Brent R. [0000-0002-3532-3868], Strappazzon, Flavie [0000-0003-0285-7449], Sun, Liming [0000-0002-0136-5605], Sun, Erwei [0000-0001-5664-513X], Szabadkai, G [0000-0002-3006-3577], Tait, Stephen W. G. [0000-0001-7697-132X], Tang, Daolin [0000-0002-1903-6180], Tavernarakis, Nektarios [0000-0002-5253-1466], Turk, Boris [0000-0002-9007-5764], Urbano, Nicoletta [0000-0003-1822-155X], Vandenabeele, Peter [0000-0002-6669-8822], Vanden Berghe, Tom [0000-0002-1633-0974], Vander Heiden, Matthew G. [0000-0002-6702-4192], Vanderluit, Jacqueline L. [0000-0002-4960-920X], Verkhratsky, A. [0000-0003-2592-9898], Villunger, Andreas [0000-0001-8259-4153], Von Karstedt, Silvia [0000-0002-7816-5919], Voss, Anne K. [0000-0002-3853-9381], Vucic, Domagoj [0000-0003-3614-8093], Vuri, Daniela [0000-0001-8693-3845], Wagner, Erwin F. [0000-0001-7872-0196], Walczak, Henning [0000-0002-6312-4591], Wallach, David [0000-0003-2724-9757], Wang, Ruoning [0000-0001-9798-8032], Weber, Achim [0000-0003-0073-3637], Yamazaki, Takahiro [0000-0002-7420-4394], Zakeri, Zahra [0000-0003-4386-8072], Zawacka-Pankau, Joanna E. [0000-0002-7415-2942], Zhivotovsky, Boris [0000-0002-2238-3482], Piacentini, Mauro [0000-0003-2919-1296], Kroemer, Guido [0000-0002-9334-4405], Galluzzi, Lorenzo [0000-0003-2257-8500 ], Vitale, Ilio, Pietrocola, Federico, Guilbaud, Emma, Aaronson, Stuart A., Abrams, John M., Dieter, Adam, Agostini, Massimiliano, Agostinis, Patrizia, Alnemri, Emad S., Altucci, Lucia, Amelio, Ivano, Andrews, David W., Aqeilan, Rami I., Arama, Eli, Baehrecke, Eric H., Balachandran, Siddharth, Bano, Daniele, Barlev, Nickolai A., Bartek, Jiri, Bazan, Nicolas G., Becker, Christoph, Bernassola, Francesca, Bertrand, Mathieu J. M., Bianchi, Marco Emilio, Blagosklonny, Mikhail V., Blander, J. Magarian, Blandino, Giovanni, Blomgren, Klas, Bomer, Christoph, Bortner, Carl D., Bove, Pierluigi, Boya, Patricia, Brenner, Catherine, Broz, Petr, Brunner, T., Damgaard, Rune Busk, Calin, George A., Campanella, Michelangelo, Candi, Eleonora, Carbone, Michele, Carmona-Gutierrez, Didac, Cecconi, Francesco, Chan, Francis K.-M., Chen, Guo‑Qiang, Chen, Quan, Chen, Youhai H., Cheng, Emily H., Chipuk, Jerry E., Cidlowski, John A., Ciechanover, Aaron, Ciliberto, Gennaro, Conrad, Marcus, Cubillos-Ruiz, Juan R., Czabotar, Peter E., D’Angiolella, Vincenzo, Daugaard, Mads, Dawson, Ted M., Dawson, Valina L., De Maria, Ruggero, De Strooper, B., Debatin, Klaus-Michael, Deberardinis, Ralph J., Degterev, Alexei, Del Sal, Giannino, Deshmukh, Mohanish, Di Virgilio, Francesco, Diederich, Marc, Dixon, Scott J., Dynlacht, Brian D., El-Deiry, Wafik S., Elrod, John W., Engeland, Kurt, Fimia, Gian María, Galassi, Claudia, Ganini, Carlo, García-Sáez, Ana J., Garg, Abhishek D., Garrido, Carmen, Gavathiotis, Evripidis, Gerlic, Motti, Ghosh, Sourav, Green, Douglas R., Greene, Lloyd A., Gronemeyer, Hinrich, Häcker, Georg, Hajnóczky, György, Hardwick, J. Marie, Haupt, Ygal, He, Sudan, Heery, David M., Hengartner, Michael O., Hetz, Claudio, Hildeman, David A., Ichijo, Hidenori, Inoue, Satoshi, Jäättelä, Marja, Janic, Ana, Joseph, Bertrand, Jost, Philipp J., Kanneganti, Thirumala-Devi, Karin, Michael, Kashkar, Hamid, Kaufmann, Thomas, Kelly, Gemma L., Kepp, Oliver, Kimchi, Adi, Kitsis, Richard N., Klionsky, Daniel J., Kluck, Ruth, Krysko, Dmitri V., Kulms, Dagmar, Kumar, Sharad, Lavandero, Sergio, Lavrik, Inna N., Lemasters, John J., Liccardi, Gianmaria, Linkermann, Andreas, Lipton, Stuart A., Lockshin, Richard A., López-Otín, Carlos, Luedde, Tom, MacFarlane, Marion, Madeo, Frank, Malorni, Walter, Manic, Gwenola, Mantovani, Roberto, Marchi, Saverio, Marine, Jean-Christophe, Martin, Seamus J., Martinou, Jean-Claude, Mastroberardino, Pier G., Medema, Jan Paul, Mehlen, Patrick, Meier, Pascal, Melino, Gerry, Melino, Sonia, Miao, Edward A., Moll, Ute M., Muñoz-Pinedo, Cristina, Murphy, Daniel J., Niklison-Chirou, Maria Victoria, Novelli, Flavia, Núñez, Gabriel, Oberst, Andrew, Ofengeim, Dimitry, Opferman, Joseph T., Oren, Moshe, Pagano, Michele, Panaretakis, Theocharis, Pasparakis, Manolis, Penninger, Josef M., Pentimalli, Francesca, Pereira, David M., Pervaiz, Shazib, Peter, Marcus E., Pinton, Paolo, Porta, Giovanni, Prehn, Jochen H. M., Puthalakath, Hamsa, Rabinovich, Gabriel A., Rajalingam, Krishnaraj, Ravinchandran, Kodi S., Rehm, Markus, Ricci, Jean-Ehrland, Rizzuto, Rosario, Robinson, Nirmal, Rodrigues, Cecilia M. P., Rotblat, Barak, Rothlin, Carla V., Rubinsztein, David C., Rudel, Thomas, Rufini, Alessandro, Ryan, Kevin M., Sarosiek, Kristopher A., Sawa, Akira, Sayan, Emre, Schroder, Kate, Scorrano, Luca, Sesti, Federico, Shao, Feng, Shi, Yufang, Sica, Giuseppe, Silke, John, Simon, Hans-Uwe, Sistigu, Antonella, Stephanou, Anastasis, Stockwell, Brent R., Strappazzon, Flavie, Strasser, Andreas, Sun, Liming, Sun, Erwei, Sun, Qiang, Szabadkai, G, Tait, Stephen W. G., Tang, Daolin, Tavernarakis, Nektarios, Troy, Carol M., Turk, Boris, Urbano, Nicoletta, Vandenabeele, Peter, Vanden Berghe, Tom, Vander Heiden, Matthew G., Vanderluit, Jacqueline L., Verkhratsky, A., Villunger, Andreas, Von Karstedt, Silvia, Voss, Anne K., Vousden, Karen H., Vucic, Domagoj, Vuri, Daniela, Wagner, Erwin F., Walczak, Henning, Wallach, David, Wang, Ruoning, Wang, Ying, Weber, Achim, Wood, Will, Yamazaki, Takahiro, Yang, Zahra, Zakeri, Zahra, Zawacka-Pankau, Joanna E., Zhang, Lin, Zhang, Haibin, Zhivotovsky, Boris, Zhou, Wenzhao, Piacentini, Mauro, Kroemer, Guido, and Galluzzi, Lorenzo
- Abstract
Apoptosis is a form of regulated cell death (RCD) that involves proteases of the caspase family. Pharmacological and genetic strategies that experimentally inhibit or delay apoptosis in mammalian systems have elucidated the key contribution of this process not only to (post-)embryonic development and adult tissue homeostasis, but also to the etiology of multiple human disorders. Consistent with this notion, while defects in the molecular machinery for apoptotic cell death impair organismal development and promote oncogenesis, the unwarranted activation of apoptosis promotes cell loss and tissue damage in the context of various neurological, cardiovascular, renal, hepatic, infectious, neoplastic and inflammatory conditions. Here, the Nomenclature Committee on Cell Death (NCCD) gathered to critically summarize an abundant pre-clinical literature mechanistically linking the core apoptotic apparatus to organismal homeostasis in the context of disease.
- Published
- 2023
10. HMGB1 released by mesothelial cells drives the development of asbestos-induced mesothelioma
- Author
-
Suarez, Joelle S., primary, Novelli, Flavia, additional, Goto, Keisuke, additional, Ehara, Michiko, additional, Steele, Mika, additional, Kim, Jin-Hee, additional, Zolondick, Alicia A., additional, Xue, Jiaming, additional, Xu, Ronghui, additional, Saito, Mai, additional, Pastorino, Sandra, additional, Minaai, Michael, additional, Takanishi, Yasutaka, additional, Emi, Mitsuru, additional, Pagano, Ian, additional, Wakeham, Andrew, additional, Berger, Thorsten, additional, Pass, Harvey I., additional, Gaudino, Giovanni, additional, Mak, Tak W., additional, Carbone, Michele, additional, and Yang, Haining, additional
- Published
- 2023
- Full Text
- View/download PDF
11. Peptide-Functionalized and Drug-Loaded Tomato Bushy Stunt Virus Nanoparticles Counteract Tumor Growth in a Mouse Model of Shh-Dependent Medulloblastoma
- Author
-
Marchetti, Luca, primary, Novelli, Flavia, additional, Tanno, Barbara, additional, Leonardi, Simona, additional, Hizam, Veronica Mohamed, additional, Arcangeli, Caterina, additional, Santi, Luca, additional, Baschieri, Selene, additional, Lico, Chiara, additional, and Mancuso, Mariateresa, additional
- Published
- 2023
- Full Text
- View/download PDF
12. Characterization of Early and Late Damage in a Mouse Model of Pelvic Radiation Disease
- Author
-
Vitali, Roberta, primary, Palone, Francesca, additional, De Stefano, Ilaria, additional, Fiorente, Chiara, additional, Novelli, Flavia, additional, Pasquali, Emanuela, additional, Fratini, Emiliano, additional, Tanori, Mirella, additional, Leonardi, Simona, additional, Tanno, Barbara, additional, Colantoni, Eleonora, additional, Soldi, Sara, additional, Galletti, Serena, additional, Grimaldi, Maria, additional, Morganti, Alessio Giuseppe, additional, Fuccio, Lorenzo, additional, Pazzaglia, Simonetta, additional, Pioli, Claudio, additional, Mancuso, Mariateresa, additional, and Vesci, Loredana, additional
- Published
- 2023
- Full Text
- View/download PDF
13. Supplementary Figure 1 from The Cytoskeleton Regulatory Protein hMena (ENAH) Is Overexpressed in Human Benign Breast Lesions with High Risk of Transformation and Human Epidermal Growth Factor Receptor-2–Positive/Hormonal Receptor–Negative Tumors
- Author
-
Di Modugno, Francesca, primary, Mottolese, Marcella, primary, Di Benedetto, Anna, primary, Conidi, Andrea, primary, Novelli, Flavia, primary, Perracchio, Letizia, primary, Venturo, Irene, primary, Botti, Claudio, primary, Jager, Elke, primary, Santoni, Angela, primary, Natali, Pier Giorgio, primary, and Nisticò, Paola, primary
- Published
- 2023
- Full Text
- View/download PDF
14. Supplementary Figure Legend from The Cytoskeleton Regulatory Protein hMena (ENAH) Is Overexpressed in Human Benign Breast Lesions with High Risk of Transformation and Human Epidermal Growth Factor Receptor-2–Positive/Hormonal Receptor–Negative Tumors
- Author
-
Di Modugno, Francesca, primary, Mottolese, Marcella, primary, Di Benedetto, Anna, primary, Conidi, Andrea, primary, Novelli, Flavia, primary, Perracchio, Letizia, primary, Venturo, Irene, primary, Botti, Claudio, primary, Jager, Elke, primary, Santoni, Angela, primary, Natali, Pier Giorgio, primary, and Nisticò, Paola, primary
- Published
- 2023
- Full Text
- View/download PDF
15. BAP1 is a novel regulator of HIF-1α
- Author
-
Bononi, Angela, primary, Wang, Qian, additional, Zolondick, Alicia A., additional, Bai, Fang, additional, Steele-Tanji, Mika, additional, Suarez, Joelle S., additional, Pastorino, Sandra, additional, Sipes, Abigail, additional, Signorato, Valentina, additional, Ferro, Angelica, additional, Novelli, Flavia, additional, Kim, Jin-Hee, additional, Minaai, Michael, additional, Takinishi, Yasutaka, additional, Pellegrini, Laura, additional, Napolitano, Andrea, additional, Xu, Ronghui, additional, Farrar, Christine, additional, Goparaju, Chandra, additional, Bassi, Cristian, additional, Negrini, Massimo, additional, Pagano, Ian, additional, Sakamoto, Greg, additional, Gaudino, Giovanni, additional, Pass, Harvey I., additional, Onuchic, José N., additional, Yang, Haining, additional, and Carbone, Michele, additional
- Published
- 2023
- Full Text
- View/download PDF
16. MiRNA-Mediated Fibrosis in the Out-of-Target Heart following Partial-Body Irradiation
- Author
-
Tanno, Barbara, primary, Novelli, Flavia, additional, Leonardi, Simona, additional, Merla, Caterina, additional, Babini, Gabriele, additional, Giardullo, Paola, additional, Kadhim, Munira, additional, Traynor, Damien, additional, Medipally, Dinesh, additional, Meade, Aidan, additional, Lyng, Fiona, additional, Tapio, Soile, additional, Marchetti, Luca, additional, Saran, Anna, additional, Pazzaglia, Simonetta, additional, and Mancuso, Mariateresa, additional
- Published
- 2022
- Full Text
- View/download PDF
17. p63 in corneal and epidermal differentiation
- Author
-
Novelli, Flavia, primary, Ganini, Carlo, additional, Melino, Gerry, additional, Nucci, Carlo, additional, Han, Yuyi, additional, Shi, Yufang, additional, Wang, Ying, additional, and Candi, Eleonora, additional
- Published
- 2022
- Full Text
- View/download PDF
18. Tomato Bushy Stunt Virus Nanoparticles as a Platform for Drug Delivery to Shh-Dependent Medulloblastoma
- Author
-
Lico, Chiara, primary, Tanno, Barbara, additional, Marchetti, Luca, additional, Novelli, Flavia, additional, Giardullo, Paola, additional, Arcangeli, Caterina, additional, Pazzaglia, Simonetta, additional, Podda, Maurizio S., additional, Santi, Luca, additional, Bernini, Roberta, additional, Baschieri, Selene, additional, and Mancuso, Mariateresa, additional
- Published
- 2021
- Full Text
- View/download PDF
19. Global mapping of cancers: The Cancer Genome Atlas and beyond
- Author
-
Ganini, Carlo, primary, Amelio, Ivano, additional, Bertolo, Riccardo, additional, Bove, Pierluigi, additional, Buonomo, Oreste Claudio, additional, Candi, Eleonora, additional, Cipriani, Chiara, additional, Di Daniele, Nicola, additional, Juhl, Hartmut, additional, Mauriello, Alessandro, additional, Marani, Carla, additional, Marshall, John, additional, Melino, Sonia, additional, Marchetti, Paolo, additional, Montanaro, Manuela, additional, Natale, Maria Emanuela, additional, Novelli, Flavia, additional, Palmieri, Giampiero, additional, Piacentini, Mauro, additional, Rendina, Erino Angelo, additional, Roselli, Mario, additional, Sica, Giuseppe, additional, Tesauro, Manfredi, additional, Rovella, Valentina, additional, Tisone, Giuseppe, additional, Shi, Yufang, additional, Wang, Ying, additional, and Melino, Gerry, additional
- Published
- 2021
- Full Text
- View/download PDF
20. Interaction between glutathione-S-transferase polymorphisms, smoking habit, and HPV infection in cervical cancer risk
- Author
-
Palma, Selena, Novelli, Flavia, Padua, Luca, Venuti, Aldo, Prignano, Grazia, Mariani, Luciano, Cozzi, Renata, Tirindelli, Donatella, and Testa, Antonella
- Published
- 2010
- Full Text
- View/download PDF
21. Single-nucleotide polymorphisms in BER and HRR genes, XRCC1 haplotypes and breast cancer risk in Caucasian women
- Author
-
Sterpone, Silvia, Mastellone, Valeria, Padua, Luca, Novelli, Flavia, Patrono, Clarice, Cornetta, Tommaso, Giammarino, Daniela, Donato, Vittorio, Testa, Antonella, and Cozzi, Renata
- Published
- 2010
- Full Text
- View/download PDF
22. Beyond DNA repair, the immunological role of PARP-1 and its siblings
- Author
-
Rosado, Maria Manuela, Bennici, Elisabetta, Novelli, Flavia, and Pioli, Claudio
- Published
- 2013
- Full Text
- View/download PDF
23. p63 Is a Promising Marker in the Diagnosis of Unusual Skin Cancer
- Author
-
Smirnov, Artem, primary, Anemona, Lucia, additional, Novelli, Flavia, additional, Piro, Cristina M., additional, Annicchiarico-Petruzzelli, Margherita, additional, Melino, Gerry, additional, and Candi, Eleonora, additional
- Published
- 2019
- Full Text
- View/download PDF
24. Chromogenic in situ hybridization to detect EGFR gene copy number in cell blocks from fine-needle aspirates of non small cell lung carcinomas and lung metastases from colo-rectal cancer
- Author
-
Terrenato Irene, Di Benedetto Anna, Siciliano Michele, Rubini Vincenza, Malfettone Andrea, Mangia Anita, Simone Giovanni, Novelli Flavia, and Mottolese Marcella
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Several studies demonstrated that epidermal growth factor receptor (EGFR) gene copy number (GCN) correlates to the response to tyrosine kinase inhibitors in non small cell lung cancer (NSCLC) and to anti-EGFR monoclonal antibodies (MoAbs) in metastatic colorectal cancer (CRC). In the presence of lung nodules, cytology is often the only possible diagnostic approach. Chromogenic in situ hybridization (CISH) is an alternative technique to fluorescence in situ hybridization (FISH), but its feasibility in detecting EGFR GCN in cell blocks from fine-needle aspiration cytology (FNAC) of lung nodules has not yet been established. Methods We evaluated the feasibility of CISH on 33 FNAC from 20 primary NSCLC (5 squamous carcinomas, 8 large cell carcinomas and 7 adenocarcinomas) and 13 lung metastases from CRC. Results Of the 33 FNAC analyzed by CISH, 27 (82%) presented a balanced increase in EGFR gene and chromosome 7 number: 10 cases (30%) showed a low polysomy, 15 (45%) a high polysomy and 2 (6%) NSCLC were amplified. No significant differences between NSCLC and CRC lung metastases were found in relation to disomic or polysomic status. In addition, no correlation between EGFR GCN and EGFR immunohistochemical overexpression was found. Furthermore, we compared CISH results with those obtained by FISH on the same samples and we found 97% overall agreement between the two assays (k = 0.78, p < 0.0001). Two cases were amplified with both assays, whereas 1 case of NSCLC was amplified by FISH only. CISH sensitivity was 67%, the specificity and positive predictive value (PPV) was 100%, and the negative predictive value (NPV) was 97%. Conclusions Our study shows that CISH is a valid method to detect EGFR GCN in cell blocks from FNAC of primary NSCLC or metastatic CRC to the lung.
- Published
- 2010
- Full Text
- View/download PDF
25. Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems
- Author
-
Benevolo Maria, Natali Pier, Martayan Aline, Fraioli Rocco, Tornambé Andrea, Sperandei Maria, Di Donato Monica, Novelli Flavia, Pietraforte Immacolata, Lombardi Alessio, Galeffi Patrizia, Mottolese Marcella, Ylera Francisco, Cantale Cristina, and Giacomini Patrizio
- Subjects
Medicine - Abstract
Abstract Background Aberrant signaling by ErbB-2 (HER 2, Neu), a member of the human Epidermal Growth Factor (EGF) receptor family, is associated with an aggressive clinical behaviour of carcinomas, particularly breast tumors. Antibodies targeting the ErbB-2 pathway are a preferred therapeutic option for patients with advanced breast cancer, but a worldwide deficit in the manufacturing capacities of mammalian cell bioreactors is foreseen. Methods Herein, we describe a multi-platform approach for the production of recombinant Single chain Fragments of antibody variable regions (ScFvs) to ErbB-2 that involves their functional expression in (a) bacteria, (b) transient as well as stable transgenic tobacco plants, and (c) a newly developed cell-free transcription-translation system. Results An ScFv (ScFv800E6) was selected by cloning immunoglobulin sequences from murine hybridomas, and was expressed and fully functional in all the expression platforms, thereby representing the first ScFv to ErbB-2 produced in hosts other than bacteria and yeast. ScFv800E6 was optimized with respect to redox synthesis conditions. Different tags were introduced flanking the ScFv800E6 backbone, with and without spacer arms, including a novel Strep II tag that outperforms conventional streptavidin-based detection systems. ScFv800E6 was resistant to standard chemical radiolabeling procedures (i.e. Chloramine T), displayed a binding ability extremely similar to that of the parental monovalent Fab' fragment, as well as a flow cytometry performance and an equilibrium binding affinity (Ka approximately 2 × 108 M-1) only slightly lower than those of the parental bivalent antibody, suggesting that its binding site is conserved as compared to that of the parental antibody molecule. ScFv800E6 was found to be compatible with routine reagents for immunohistochemical staining. Conclusion ScFv800E6 is a useful reagent for in vitro biochemical and immunodiagnostic applications in oncology, and a candidate for future in vivo studies.
- Published
- 2006
- Full Text
- View/download PDF
26. Asbestos induces mesothelial cell transformation via HMGB1-driven autophagy.
- Author
-
Jiaming Xue, Patergnani, Simone, Giorgi, Carlotta, Suarez, Joelle, Keisuke Goto, Bononi, Angela, Tanji, Mika, Novelli, Flavia, Pastorino, Sandra, Ronghui Xu, Caroccia, Natascia, Dogan, A. Umran, Pass, Harvey I., Tognon, Mauro, Pinton, Paolo, Gaudino, Giovanni, Tak W. Mak, Carbone, Michele, and Haining Yang
- Subjects
CELL transformation ,ASBESTOS ,CELL death ,DNA damage - Abstract
Asbestos causes malignant transformation of primary human mesothelial cells (HM), leading to mesothelioma. The mechanisms of asbestos carcinogenesis remain enigmatic, as exposure to asbestos induces HM death. However, some asbestos-exposed HM escape cell death, accumulate DNA damage, and may become transformed. We previously demonstrated that, upon asbestos exposure, HM and reactive macrophages releases the high mobility group box 1 (HMGB1) protein that becomes detectable in the tissues near asbestos deposits where HMGB1 triggers chronic inflammation. HMGB1 is also detectable in the sera of asbestos-exposed individuals and mice. Searching for additional biomarkers, we found higher levels of the autophagy marker ATG5 in sera from asbestos-exposed individuals compared to unexposed controls. As we investigated the mechanisms underlying this finding, we discovered that the release of HMGB1 upon asbestos exposure promoted autophagy, allowing a higher fraction of HM to survive asbestos exposure. HMGB1 silencing inhibited autophagy and increased asbestos-induced HM death, thereby decreasing asbestos-induced HM transformation. We demonstrate that autophagy was induced by the cytoplasmic and extracellular fractions of HMGB1 via the engagement of the RAGE receptor and Beclin 1 pathway, while nuclear HMGB1 did not participate in this process. We validated our findings in a novel unique mesothelial conditional HMGB1-knockout (HMGB1-cKO) mouse model. Compared to HMGB1 wild-type mice, mesothelial cells from HMGB1-cKO mice showed significantly reduced autophagy and increased cell death. Autophagy inhibitors chloroquine and desmethylclomipramine increased cell death and reduced asbestos-driven foci formation. In summary, HMGB1 released upon asbestos exposure induces autophagy, promoting HM survival and malignant transformation. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
27. Enhancing the Secretion of a Glyco-Engineered Anti-CD20 scFv-Fc Antibody in Hairy Root Cultures
- Author
-
Lonoce, Chiara, primary, Marusic, Carla, additional, Morrocchi, Elena, additional, Salzano, Anna Maria, additional, Scaloni, Andrea, additional, Novelli, Flavia, additional, Pioli, Claudio, additional, Feeney, Mistianne, additional, Frigerio, Lorenzo, additional, and Donini, Marcello, additional
- Published
- 2018
- Full Text
- View/download PDF
28. N-glycan engineering of a plant-produced anti-CD20-hIL-2 immunocytokine significantly enhances its effector functions
- Author
-
Marusic, Carla, primary, Pioli, Claudio, additional, Stelter, Szymon, additional, Novelli, Flavia, additional, Lonoce, Chiara, additional, Morrocchi, Elena, additional, Benvenuto, Eugenio, additional, Salzano, Anna Maria, additional, Scaloni, Andrea, additional, and Donini, Marcello, additional
- Published
- 2017
- Full Text
- View/download PDF
29. Zinc-finger proteins in health and disease
- Author
-
Cassandri, Matteo, primary, Smirnov, Artem, additional, Novelli, Flavia, additional, Pitolli, Consuelo, additional, Agostini, Massimiliano, additional, Malewicz, Michal, additional, Melino, Gerry, additional, and Raschellà, Giuseppe, additional
- Published
- 2017
- Full Text
- View/download PDF
30. EFFECTS OF IN VIVO PROTON IRRADIATION ON MOUSE T AND B LYMPHOCYTES
- Author
-
Novelli, Flavia, primary, Vadrucci, Monia, additional, Rosado, Maria Manuela, additional, Picardi, Luigi, additional, Benvenuto, Eugenio, additional, and Pioli, Claudio, additional
- Published
- 2017
- Full Text
- View/download PDF
31. Metabolic pathways regulated by p63
- Author
-
Candi, Eleonora, primary, Smirnov, Artem, additional, Panatta, Emanuele, additional, Lena, Anna Maria, additional, Novelli, Flavia, additional, Mancini, Mara, additional, Viticchiè, Giuditta, additional, Piro, Maria Cristina, additional, Di Daniele, Nicola, additional, Annicchiarico-Petruzzelli, Margherita, additional, and Melino, Gerry, additional
- Published
- 2017
- Full Text
- View/download PDF
32. Molecular genetic alterations in egfr CA-SSR-1 microsatellite and egfr copy number changes are associated with aggressiveness in thymoma
- Author
-
Conti, Salvatore, Gallo, Enzo, Sioletic, Stefano, Facciolo, Francesco, Palmieri, Giovannella, Lauriola, Libero, Evoli Stampanoni-B, Amelia, Martucci, Robert, Di Benedetto, Anna, Novelli, Flavia, Giannarelli, Diana, Deriu, Gloria, Granone, Pierluigi, Ottaviano, Margaret, Muti, Paola, Pescarmona, Edoardo, Marino, Mirella, Lauriola, Libero (ORCID:0000-0003-0481-5138), Evoli, Amelia (ORCID:0000-0003-0282-8787), Granone, Pierluigi (ORCID:0000-0002-8826-3045), Conti, Salvatore, Gallo, Enzo, Sioletic, Stefano, Facciolo, Francesco, Palmieri, Giovannella, Lauriola, Libero, Evoli Stampanoni-B, Amelia, Martucci, Robert, Di Benedetto, Anna, Novelli, Flavia, Giannarelli, Diana, Deriu, Gloria, Granone, Pierluigi, Ottaviano, Margaret, Muti, Paola, Pescarmona, Edoardo, Marino, Mirella, Lauriola, Libero (ORCID:0000-0003-0481-5138), Evoli, Amelia (ORCID:0000-0003-0282-8787), and Granone, Pierluigi (ORCID:0000-0002-8826-3045)
- Abstract
Background: The key role of egfr in thymoma pathogenesis has been questioned following the failure in identifying recurrent genetic alterations of egfr coding sequences and relevant egfr amplification rate. We investigated the role of the non-coding egfr CA simple sequence repeat 1 (CA-SSR-1) in a thymoma case series. Methods: We used sequencing and egfr-fluorescence in situ hybridization (FISH) to genotype 43 thymomas; (I) for polymorphisms and somatic loss of heterozygosity of the non-coding egfr CA-SSR-1 microsatellite and (II) for egfr gene copy number changes. Results: We found two prevalent CA-SSR-1 genotypes: A homozygous 16 CA repeat and a heterozygous genotype, bearing alleles with 16 and 20 CA repeats. The average combined allele length was correlated with tumor subtype: Shorter sequences were significantly associated with the more aggressive WHO thymoma subtype group including B2/B3, B3 and B3/C histotypes. Four out of 29 informative cases analysed for somatic CA-SSR-1 loss of heterozygosity showed allelic imbalance (AI), 3/4 with loss of the longer allele. By egfr-FISH analysis, 9 out of 33 cases were FISH positive. Moreover, the two integrated techniques demonstrated that 3 out of 4 CA-SSR-1-AI positive cases with short allele relative prevalence showed significantly low or high chromosome 7 "polysomy"/increased gene copy number by egfr-FISH. Conclusions: Our molecular and genetic and follow up data indicated that CA-SSR-1-allelic imbalance with short allele relative prevalence significantly correlated with EGFR 3+ immunohistochemical score, increased egfr Gene Copy Number, advanced stage and with relapsing/metastatic behaviour in thymomas.
- Published
- 2016
33. Collaborative project on the evaluation of radiation-induced DNA damage and DNA repair genes polymorphisms in relation to radiotherapy adverse effects
- Author
-
CORNETTA, TOMMASO, STERPONE, Silvia, COZZI, Renata, PALMA SELENA, POGGIOLI TOMMASO, NOVELLI FLAVIA, MASTELLONE VALERIA, GIAMMARINO DANIELA, DONATO VITTORIO, TESTA ANTONELLA, Cornetta, Tommaso, Sterpone, Silvia, Palma, Selena, Poggioli, Tommaso, Novelli, Flavia, Mastellone, Valeria, Giammarino, Daniela, Donato, Vittorio, Testa, Antonella, and Cozzi, Renata
- Published
- 2008
34. Molecular genetic alterations in egfr CA-SSR-1 microsatellite and egfr copy number changes are associated with aggressiveness in thymoma
- Author
-
Conti, Salvatore, primary, Gallo, Enzo, additional, Sioletic, Stefano, additional, Facciolo, Francesco, additional, Palmieri, Giovannella, additional, Lauriola, Libero, additional, Evoli, Amelia, additional, Martucci, Robert, additional, Di Benedetto, Anna, additional, Novelli, Flavia, additional, Giannarelli, Diana, additional, Deriu, Gloria, additional, Granone, Pierluigi, additional, Ottaviano, Margaret, additional, Muti, Paola, additional, Pescarmona, Edoardo, additional, and Marino, Mirella, additional
- Published
- 2016
- Full Text
- View/download PDF
35. Amino-terminal residues of ΔNp63, mutated in ectodermal dysplasia, are required for its transcriptional activity
- Author
-
Lena, Anna Maria, primary, Duca, Sara, additional, Novelli, Flavia, additional, Melino, Sonia, additional, Annicchiarico-Petruzzelli, Margherita, additional, Melino, Gerry, additional, and Candi, Eleonora, additional
- Published
- 2015
- Full Text
- View/download PDF
36. Poly(ADP-ribose)polymerase-1 (PARP-1) negatively regulates differentiation and stability of regulatory T cells
- Author
-
Pioli Claudio, Sambucci Manolo, Novelli Flavia, Laudisi Federica, and Bennici Elisabetta
- Subjects
Chemistry ,Poly ADP ribose polymerase ,Immunology ,Immunology and Allergy ,Molecular biology - Published
- 2013
37. N‐glycan engineering of a plant‐produced anti‐CD20‐hIL‐2 immunocytokine significantly enhances its effector functions.
- Author
-
Marusic, Carla, Pioli, Claudio, Stelter, Szymon, Novelli, Flavia, Lonoce, Chiara, Morrocchi, Elena, Benvenuto, Eugenio, Salzano, Anna Maria, Scaloni, Andrea, and Donini, Marcello
- Abstract
Abstract: Anti‐CD20 recombinant antibodies are among the most promising therapeutics for the treatment of B‐cell malignancies such as non‐Hodgkin lymphomas. We recently demonstrated that an immunocytokine (2B8‐Fc‐hIL2), obtained by fusing an anti‐CD20 scFv‐Fc antibody derived from C2B8 mAb (rituximab) to the human interleukin 2 (hIL‐2), can be efficiently produced in
Nicotiana benthamiana plants. The purified immunocytokine (IC) bearing a typical plant protein N‐glycosylation profile showed a CD20 binding activity comparable to that of rituximab and was efficient in eliciting antibody‐dependent cell‐mediated cytotoxicity (ADCC) of human PBMC against Daudi cells, indicating its fuctional integrity. In this work, the immunocytokine devoid of the typical xylose/fucose N‐glycosylation plant signature (IC‐ΔXF) and the corresponding scFv‐Fc‐ΔXF antibody not fused to the cytokine, were obtained in a glyco‐engineered ΔXylT/FucTN. benthamiana line. Purification yields from agroinfiltrated plants amounted to 20–35 mg/kg of leaf fresh weight. When assayed for interaction with FcγRI and FcγRIIIa, IC‐ΔXF exhibited significantly enhanced binding affinities if compared to the counterpart bearing the typical plant protein N‐glycosylation profile (IC) and to rituximab. The glyco‐engineered recombinant molecules also exhibited a strongly improved ADCC and complement‐dependent cytotoxicity (CDC). Notably, our results demonstrate a reduced C1q binding of xylose/fucose carrying IC and scFv‐Fc compared to versions that lack these sugar moieties. These results demonstrate that specific N‐glycosylation alterations in recombinant products can dramatically affect the effector functions of the immunocytokine, resulting in an overall improvement of the biological functions and consequently of the therapeutic potential. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
38. Production of an active anti‐CD20‐hIL‐2 immunocytokine in Nicotiana benthamiana
- Author
-
Marusic, Carla, primary, Novelli, Flavia, additional, Salzano, Anna M., additional, Scaloni, Andrea, additional, Benvenuto, Eugenio, additional, Pioli, Claudio, additional, and Donini, Marcello, additional
- Published
- 2015
- Full Text
- View/download PDF
39. Production of an active anti-CD20-hIL-2 immunocytokine in Nicotiana benthamiana.
- Author
-
Marusic, Carla, Novelli, Flavia, Salzano, Anna M., Scaloni, Andrea, Benvenuto, Eugenio, Pioli, Claudio, and Donini, Marcello
- Subjects
- *
NICOTIANA benthamiana , *CD20 antigen , *CYTOKINES , *LYMPHOMA treatment , *CANCER relapse , *LYMPHOMAS , *PATIENTS - Abstract
Anti-CD20 murine or chimeric antibodies (Abs) have been used to treat non-Hodgkin lymphomas (NHLs) and other diseases characterized by overactive or dysfunctional B cells. Anti-CD20 Abs demonstrated to be effective in inducing regression of B-cell lymphomas, although in many cases patients relapse following treatment. A promising approach to improve the outcome of mAb therapy is the use of anti-CD20 antibodies to deliver cytokines to the tumour microenvironment. In particular, IL-2-based immunocytokines have shown enhanced antitumour activity in several preclinical studies. Here, we report on the engineering of an anti-CD20-human interleukin-2 (hIL- 2) immunocytokine (2B8-Fc-hIL2) based on the C2B8 mAb (Rituximab) and the resulting ectopic expression in Nicotiana benthamiana. The scFv-Fc-engineered immunocytokine is fully assembled in plants with minor degradation products as assessed by SDS-PAGE and gel filtration. Purification yields using protein-A affinity chromatography were in the range of 15-20 mg/kg of fresh leaf weight (FW). Glycopeptide analysis confirmed the presence of a highly homogeneous plant-type glycosylation. 2B8-Fc-hIL2 and the cognate 2B8-Fc antibody, devoid of hIL-2, were assayed by flow cytometry on Daudi cells revealing a CD20 binding activity comparable to that of Rituximab and were effective in eliciting antibody-dependent cell-mediated cytotoxicity of human PBMC versus Daudi cells, demonstrating their functional integrity. In 2B8-Fc-hIL2, IL-2 accessibility and biological activity were verified by flow cytometry and cell proliferation assay. To our knowledge, this is the first example of a recombinant immunocytokine based on the therapeutic Rituximab antibody scaffold, whose expression in plants may be a valuable tool for NHLs treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
40. Chromogenic in situ hybridization to detect EGFR gene copy number in cell blocks from fine-needle aspirates of non small cell lung carcinomas and lung metastases from colo-rectal cancer
- Author
-
Simone, Giovanni, primary, Mangia, Anita, additional, Malfettone, Andrea, additional, Rubini, Vincenza, additional, Siciliano, Michele, additional, Di Benedetto, Anna, additional, Terrenato, Irene, additional, Novelli, Flavia, additional, and Mottolese, Marcella, additional
- Published
- 2010
- Full Text
- View/download PDF
41. A divergent role for estrogen receptor-beta in node-positive and node-negative breast cancer classified according to molecular subtypes: an observational prospective study
- Author
-
Novelli, Flavia, primary, Milella, Michele, additional, Melucci, Elisa, additional, Di Benedetto, Anna, additional, Sperduti, Isabella, additional, Perrone-Donnorso, Raffaele, additional, Perracchio, Letizia, additional, Venturo, Irene, additional, Nisticò, Cecilia, additional, Fabi, Alessandra, additional, Buglioni, Simonetta, additional, Natali, Pier Giorgio, additional, and Mottolese, Marcella, additional
- Published
- 2008
- Full Text
- View/download PDF
42. P1-161: Nuclear and cytoplasmic cellular distribution of survivin as survival predictor in resected non-small-cell lung cancer
- Author
-
Bria, Emilio, primary, Visca, Paolo, additional, Novelli, Flavia, additional, Antoniani, Barbara, additional, Perrone-Donnorso, Raffaele, additional, Sperduti, Isabella, additional, Facciolo, Francesco, additional, Milella, Michele, additional, Cognetti, Francesco, additional, and Mottolese, Marcella, additional
- Published
- 2007
- Full Text
- View/download PDF
43. Ten years of experience with weekly chemotherapy in metastatic breast cancer patients: multivariate analysis of prognostic factors
- Author
-
Nisticò, Cecilia, primary, Cuppone, Federica, additional, Bria, Emilio, additional, Fornier, Monica, additional, Giannarelli, Diana, additional, Mottolese, Marcella, additional, Novelli, Flavia, additional, Natoli, Guido, additional, Cognetti, Francesco, additional, and Terzoli, Edmondo, additional
- Published
- 2006
- Full Text
- View/download PDF
44. Chromogenic In Situ Hybridization to Detect HER-2/neu Gene Amplification in Histological and ThinPrep®-Processed Breast Cancer Fine-Needle Aspirates: A Sensitive and Practical Method in the Trastuzumab Era
- Author
-
Vocaturo, Amina, primary, Novelli, Flavia, additional, Benevolo, Maria, additional, Piperno, Giulia, additional, Marandino, Ferdinando, additional, Cianciulli, Anna Maria, additional, Merola, Roberta, additional, Donnorso, Raffaele Perrone, additional, Sperduti, Isabella, additional, Buglioni, Simonetta, additional, and Mottolese, Marcella, additional
- Published
- 2006
- Full Text
- View/download PDF
45. Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems
- Author
-
Galeffi, Patrizia, primary, Lombardi, Alessio, additional, Pietraforte, Immacolata, additional, Novelli, Flavia, additional, Di Donato, Monica, additional, Sperandei, Maria, additional, Tornambé, Andrea, additional, Fraioli, Rocco, additional, Martayan, Aline, additional, Natali, Pier Giorgio, additional, Benevolo, Maria, additional, Mottolese, Marcella, additional, Ylera, Francisco, additional, Cantale, Cristina, additional, and Giacomini, Patrizio, additional
- Published
- 2006
- Full Text
- View/download PDF
46. The Cytoskeleton Regulatory Protein hMena (ENAH) Is Overexpressed in Human Benign Breast Lesions with High Risk of Transformation and Human Epidermal Growth Factor Receptor-2–Positive/Hormonal Receptor–Negative Tumors
- Author
-
Di Modugno, Francesca, primary, Mottolese, Marcella, additional, Di Benedetto, Anna, additional, Conidi, Andrea, additional, Novelli, Flavia, additional, Perracchio, Letizia, additional, Venturo, Irene, additional, Botti, Claudio, additional, Jager, Elke, additional, Santoni, Angela, additional, Natali, Pier Giorgio, additional, and Nisticò, Paola, additional
- Published
- 2006
- Full Text
- View/download PDF
47. Enhancing the Secretion of a Glyco‐Engineered Anti‐CD20 scFv‐Fc Antibody in Hairy Root Cultures.
- Author
-
Lonoce, Chiara, Marusic, Carla, Morrocchi, Elena, Salzano, Anna Maria, Scaloni, Andrea, Novelli, Flavia, Pioli, Claudio, Feeney, Mistianne, Frigerio, Lorenzo, and Donini, Marcello
- Published
- 2019
- Full Text
- View/download PDF
48. Prognostic value of HER2 and progesterone receptor expression in endometrial carcinoma with positive peritoneal washing
- Author
-
Benevolo, Maria, Vocaturo, Amina, Novelli, Flavia, Mariani, Luciano, Vocaturo, Giuseppe, Cianciulli, Anna Maria, Marandino, Ferdinando, Perrone-Donnorso, Raffaele, Diana Giannarelli, Natali, Pier Giorgio, and Mottolese, Marcella
49. Nuclear and cytoplasmic cellular distribution of survivin as survival predictor in resected non-small-cell lung cancer.
- Author
-
Bria, Emilio, Visca, Paolo, Novelli, Flavia, Antoniani, Barbara, Perrone-Donnorso, Raffaele, Sperduti, Isabella, Facciolo, Francesco, Milella, Michele, Cognetti, Francesco, and Mottolese, Marcella
- Published
- 2007
- Full Text
- View/download PDF
50. Prognostic value of HER2 and progesterone receptor expression in endometrial carcinoma with positive peritoneal washing.
- Author
-
Benevolo M, Vocaturo A, Novelli F, Mariani L, Vocaturo G, Cianciulli AM, Marandino F, Perrone-Donnorso R, Giannarelli D, Natali PG, and Mottolese M
- Subjects
- Adult, Aged, Aged, 80 and over, Endometrial Neoplasms enzymology, Female, Follow-Up Studies, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Middle Aged, Peritoneal Cavity pathology, Receptors, Estrogen biosynthesis, Survival Rate, Endometrial Neoplasms metabolism, Endometrial Neoplasms pathology, Receptor, ErbB-2 biosynthesis, Receptors, Progesterone biosynthesis
- Abstract
Background: Although the majority of endometrial cancer (EC) patients can be cured by surgery, unexpected recurrent disease may also occur in early stage patients. In the present study, whether or not the analysis of multiple biopathological parameters might lead to more accurate predictions of the clinical outcome of EC patients with long-term follow-up (FU) was investigated., Patients and Methods: Estrogen and progesterone receptor (ER and PgR) positivity and HER2 overexpression by immunohistochemistry were evaluated. The peritoneal washings (PWs) were analyzed by cytology and immunocytochemistry employing AR-3 and B72.3 monoclonal antibodies., Results: The patients with positive PW and HER2 positive tumors showed shorter overall survival compared to those bearing HER2 negative tumors (p =0.004). HER2 overexpression also influenced the patient outcome in the group with tumors lacking PgR (p = 0.004). At multivariate analysis PgR and HER2 overexpression emerged as independent prognostic factors., Conclusion: The combined analysis of these biopathological markers could provide useful information for the selection of patients to be enrolled in innovative therapeutic strategies.
- Published
- 2007
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.