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1. Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene

Author

Nozawa-Inoue, Mamie, Jien, Mercy, Hamilton, Nicholas S., Stewart, Valley, Scow, Kate M., and Hristova, Krassimira R.

Subjects
Ammonium perchlorate -- Chemical properties, Ammonium perchlorate -- Environmental aspects, Polymerase chain reaction -- Usage, Soil microbiology -- Genetic aspects, Soil microbiology -- Environmental aspects, Biological sciences
Abstract

The designing of pcrA gene based real-time quantitative PCR assay for detection of perchlorate-reducing bacteria in soil microbial communities is described. Partial sequencing of pcrA gene showed differences in the composition of perchlorate-reducing bacterial communities when exposed to various electron donors.

Published
2008

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