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27 results on '"Ntafis, Vasileios"'

1. INFRAFRONTIER quality principles in systemic phenotyping

Author

Ehlich, Hilke, Cater, Heather L., Flenniken, Ann M., Goncalves Da Cruz, Isabelle, Mura, Anne-Marie, Ntafis, Vasileios, Raess, Michael, Selloum, Mohammed, Stoeger, Claudia, Suchanova, Sarka, Vuolteenaho, Reetta, Brown, Steve D. M., Hérault, Yann, Hinttala, Reetta, Hrabě de Angelis, Martin, Kollias, George, Kontoyiannis, Dimitris L., Malissen, Bernard, McKerlie, Colin, Sedláček, Radislav, Wells, Sara E., Zarubica, Ana, Rozman, Jan, and Sorg, Tania

Published
2022
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2. PIK3C[delta] expression by fibroblasts promotes triple-negative breast cancer progression

Author

Gagliano, Teresa, Shah, Kalpit, Gargani, Sofia, Lao, Liyan, Alsaleem, Mansour, Chen, Jianing, Ntafis, Vasileios, Huang, Penghan, Ditsiou, Angeliki, Vella, Viviana, Yadav, Kritika, Bienkowska, Kamila, Bresciani, Giulia, Kang, Kai, Li, Leping, Carter, Philip, Benstead-Hume, Graeme, OHanlon, Timothy, Dean, Michael, Pearl, Frances M.G., Lee, Soo-Chin, Rakha, Emad A., Green, Andrew R., Kontoyiannis, Dimitris L., Song, Erwei, Stebbing, Justin, and Giamas, Georgios

Subjects
Genetic engineering -- Analysis, Idelalisib -- Analysis, Breast cancer -- Development and progression, Cancer metastasis -- Development and progression, RNA -- Analysis, Health care industry
Abstract

As there is growing evidence for the tumor microenvironment's role in tumorigenesis, we investigated the role of fibroblast-expressed kinases in triple-negative breast cancer (TNBC). Using a high-throughput kinome screen combined with 3D invasion assays, we identified fibroblast-expressed PIK3C[delta] (f- PIK3C[delta]) as a key regulator of cancer progression. Although PIK3C[delta] was expressed in primary fibroblasts derived from TNBC patients, it was barely detectable in breast cancer (BC) cell lines. Genetic and pharmacological gain- and loss-of-function experiments verified the contribution of f-PIK3C[delta] in TNBC cell invasion. Integrated secretomics and transcriptomics analyses revealed a paracrine mechanism via which f-PIK3C[delta] confers its protumorigenic effects. Inhibition of f-PIK3C[delta] promoted the secretion of factors, including PLGF and BDNF, that led to upregulation of NR4A1 in TNBC cells, where it acts as a tumor suppressor. Inhibition of PIK3C[delta] in an orthotopic BC mouse model reduced tumor growth only after inoculation with fibroblasts, indicating a role of f-PIK3C[delta] in cancer progression. Similar results were observed in the MMTV-PyMT transgenic BC mouse model, along with a decrease in tumor metastasis, emphasizing the potential immune-independent effects of PIK3C[delta] inhibition. Finally, analysis of BC patient cohorts and TCGA data sets identified f-PIK3C[delta] (protein and mRNA levels) as an independent prognostic factor for overall and disease-free survival, highlighting it as a therapeutic target for TNBC., Introduction Triple-negative breast cancer (TNBC; [ER.sup.-], [PR.sup.-], [HER2.sup.-]) represents a molecularly diverse and highly heterogeneous subtype of breast cancer (BC) (15%-20%) with a poor prognosis and high rates of recurrence [...]

Published
2020
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4. Validation of a Loop-Mediated Amplification/ISO 6579-Based Method for Analysing Soya Meal for the Presence of Salmonella enterica

Author

D’Agostino, Martin, Robles, Susana, Hansen, Flemming, Ntafis, Vasileios, Ikonomopoulos, John, Kokkinos, Petros, Alvarez-Ordonez, Avelino, Jordan, Kieran, Delibato, Elisabetta, Kukier, Elżbieta, Sieradzki, Zbigniew, Kwiatek, Krzysztof, Milanov, Dubravka, Petrović, Tamaš, Gonzalez-Garcia, Patricia, Lazaro, David Rodriguez, Jackson, Emily E., Forsythe, Stephen J., O’Brien, Lorna, and Cook, Nigel

Published
2016
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5. The RNA binding protein HuR is a gatekeeper of liver homeostasis

Author

Subramanian, Pallavi, Gargani, Sofia, Palladini, Alessandra, Chatzimike, Margarita, Grzybek, Michal, Peitzsch, Mirko, Papanastasiou, Anastasios D, Pyrina, Iryna, Ntafis, Vasileios, Gercken, Bettina, Lesche, Mathias, Petzold, Andreas, Sinha, Anupam, Nati, Marina, Thangapandi, Veera Raghavan, Kourtzelis, Ioannis, Andreadou, Margarita, Witt, Anke, Dahl, Andreas, Burkhardt, Ralph, Haase, Robert, Domingues, António Miguel de Jesus, Henry, Ian, Zamboni, Nicola, Mirtschink, Peter, Chung, Kyoung-Jin, Hampe, Jochen, Coskun, Ünal, Kontoyiannis, Dimitris L, and Chavakis, Triantafyllos

Subjects
Elavl1, Fxr, Hur, Nafld, Nash, Rna-binding Protein, Bile Acid, Hepatocellular Carcinoma, Lipid Metabolism, Liver, Steatosis, Triglycerides, digestive system diseases
Abstract

BACKGROUND AND AIMS: Non-alcoholic fatty liver disease (NAFLD) is initiated by steatosis and can progress via fibrosis and cirrhosis to hepatocellular carcinoma (HCC). The RNA binding protein HuR controls RNAs at the posttranscriptional level; hepatocyte HuR has been implicated in the regulation of diet-induced hepatic steatosis. The present study aimed to understand the role of hepatocyte-HuR in NAFLD development and progression to fibrosis and HCC. APPROACH AND RESULTS: Hepatocyte-specific HuR-deficient mice and control HuR-sufficient mice were fed either a normal diet or a NAFLD-inducing diet. Hepatic lipid accumulation, inflammation, fibrosis and HCC development were studied by histology, flow cytometry, quantitative PCR and RNA sequencing. The liver lipidome was characterized by lipidomics analysis and the HuR-RNA interactions in the liver were mapped by RNA immunoprecipitation-sequencing. Hepatocyte-specific HuR-deficient mice displayed spontaneous hepatic steatosis and fibrosis predisposition, compared to control HuR-sufficient mice. On a NAFLD-inducing diet, hepatocyte-specific HuR-deficiency resulted in exacerbated inflammation, fibrosis and HCC-like tumor development. A multi-omic approach, including lipidomics, transcriptomics and RNA-immunoprecipitation sequencing revealed that HuR orchestrates a protective network of hepatic-metabolic and lipid homeostasis-maintaining pathways. Consistently, HuR-deficient livers accumulated, already at steady-state, a triglyceride signature resembling that of NAFLD livers. Moreover, upregulation of Spp1 and its product osteopontin mediated, at least partially, the fibrosis development in hepatocyte-specific HuR deficiency on a NAFLD-inducing diet, as shown by experiments utilizing antibody blockade of osteopontin. CONCLUSIONS: HuR is a gatekeeper of liver homeostasis preventing NAFLD-related fibrosis and HCC, suggesting that the HuR-dependent network could be exploited therapeutically.

Published
2022

6. Inactivation of AUF1 in Myeloid Cells Protects From Allergic Airway and Tumor Infiltration and Impairs the Adenosine-Induced Polarization of Pro-Angiogenic Macrophages

Author

Gargani, Sofia, primary, Lourou, Niki, additional, Arapatzi, Christina, additional, Tzanos, Dimitris, additional, Saridaki, Marania, additional, Dushku, Esmeralda, additional, Chatzimike, Margarita, additional, Sidiropoulos, Nikolaos D., additional, Andreadou, Margarita, additional, Ntafis, Vasileios, additional, Hatzis, Pantelis, additional, Kostourou, Vassiliki, additional, and Kontoyiannis, Dimitris L., additional

Published
2022
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7. Lung tumor MHCII immunity depends on in situ antigen presentation by fibroblasts

Author

Kerdidani, Dimitra, primary, Aerakis, Emmanouil, additional, Verrou, Kleio-Maria, additional, Angelidis, Ilias, additional, Douka, Katerina, additional, Maniou, Maria-Anna, additional, Stamoulis, Petros, additional, Goudevenou, Katerina, additional, Prados, Alejandro, additional, Tzaferis, Christos, additional, Ntafis, Vasileios, additional, Vamvakaris, Ioannis, additional, Kaniaris, Evangelos, additional, Vachlas, Konstantinos, additional, Sepsas, Evangelos, additional, Koutsopoulos, Anastasios, additional, Potaris, Konstantinos, additional, and Tsoumakidou, Maria, additional

Published
2022
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8. The RNA binding protein human antigen R is a gatekeeper of liver homeostasis

Author

Subramanian, Pallavi, primary, Gargani, Sofia, additional, Palladini, Alessandra, additional, Chatzimike, Margarita, additional, Grzybek, Michal, additional, Peitzsch, Mirko, additional, Papanastasiou, Anastasios D., additional, Pyrina, Iryna, additional, Ntafis, Vasileios, additional, Gercken, Bettina, additional, Lesche, Mathias, additional, Petzold, Andreas, additional, Sinha, Anupam, additional, Nati, Marina, additional, Thangapandi, Veera Raghavan, additional, Kourtzelis, Ioannis, additional, Andreadou, Margarita, additional, Witt, Anke, additional, Dahl, Andreas, additional, Burkhardt, Ralph, additional, Haase, Robert, additional, Domingues, António Miguel de Jesus, additional, Henry, Ian, additional, Zamboni, Nicola, additional, Mirtschink, Peter, additional, Chung, Kyoung‐Jin, additional, Hampe, Jochen, additional, Coskun, Ünal, additional, Kontoyiannis, Dimitris L., additional, and Chavakis, Triantafyllos, additional

Published
2021
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10. INFRAFRONTIER quality principles in systemic phenotyping

Author

Ehlich, Hilke, primary, Cater, Heather L., additional, Flenniken, Ann M., additional, Goncalves Da Cruz, Isabelle, additional, Mura, Anne-Marie, additional, Ntafis, Vasileios, additional, Raess, Michael, additional, Selloum, Mohammed, additional, Stoeger, Claudia, additional, Suchanova, Sarka, additional, Vuolteenaho, Reetta, additional, Brown, Steve D. M., additional, Hérault, Yann, additional, Hinttala, Reetta, additional, Hrabě de Angelis, Martin, additional, Kollias, George, additional, Kontoyiannis, Dimitris L., additional, Malissen, Bernard, additional, McKerlie, Colin, additional, Sedláček, Radislav, additional, Wells, Sara E., additional, Zarubica, Ana, additional, Rozman, Jan, additional, and Sorg, Tania, additional

Published
2021
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14. The RNA binding protein human antigen R is a gatekeeper of liver homeostasis.

Author

Subramanian, Pallavi, Gargani, Sofia, Palladini, Alessandra, Chatzimike, Margarita, Grzybek, Michal, Peitzsch, Mirko, Papanastasiou, Anastasios D., Pyrina, Iryna, Ntafis, Vasileios, Gercken, Bettina, Lesche, Mathias, Petzold, Andreas, Sinha, Anupam, Nati, Marina, Thangapandi, Veera Raghavan, Kourtzelis, Ioannis, Andreadou, Margarita, Witt, Anke, Dahl, Andreas, and Burkhardt, Ralph

Published
2022
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16. Global kinome silencing combined with 3D invasion screening of the tumor microenvironment identifies fibroblast-expressed PIK3Cδ involvement in triple-negative breast cancer progression

Author

Gagliano, Teresa, primary, Shah, Kalpit, additional, Gargani, Sofia, additional, Lao, Liyan, additional, Alsaleem, Mansour, additional, Chen, Jianing, additional, Ntafis, Vasileios, additional, Huang, Penghan, additional, Ditsiou, Angeliki, additional, Vella, Viviana, additional, Yadav, Kritika, additional, Bienkowska, Kamila, additional, Bresciani, Giulia, additional, Kang, Kai, additional, Li, Leping, additional, Carter, Philip, additional, Benstead-Hum, Graeme, additional, O’Hanlon, Timothy, additional, Dean, Michael, additional, Pearl, Frances M.G., additional, Lee, Soo-Chin, additional, Rakha, Emad A, additional, Green, Andrew R, additional, Kontoyiannis, Dimitris L., additional, Song, Erwei, additional, Stebbing, Justin, additional, and Giamas, Georgios, additional

Published
2019
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17. Phylogenetic and molecular characterization of equine H3N8 influenza viruses from Greece (2003 and 2007): Evidence for reassortment between evolutionary lineages

Author

Kanellos Theo, Ntafis Vasileios, Fragkiadaki Eirini, Bountouri Maria, and Xylouri Eftychia

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background For first time in Greece equine influenza virus infection was confirmed, by isolation and molecular analysis, as the cause of clinical respiratory disease among unvaccinated horses during 2003 and 2007 outbreaks. Methods Equine influenza virus (EIV) H3N8 was isolated in MDCK cells from 30 nasal swabs from horses with acute respiratory disease, which were tested positive by Directigen Flu A. Isolation was confirmed by haemagglutination assay and RT-PCR assay of the M, HA and NA gene. Results HA sequences of the Greek isolates appeared to be more closely related to viruses isolated in early 1990s in Europe. These results suggested that viruses with fewer changes than those on the main evolutionary lineage may continue to circulate. On the other hand, analysis of deduced NA amino acid sequences were more closely related to viruses isolated in outbreaks in Europe and Asia during 2003-2007. Phylogenetic analysis characterized the Greek isolates as a member of the Eurasian lineage by the haemagglutinin (HA) protein alignment, but appeared to be a member of the Florida sublineage clade 2 by the neuraminidase (NA) protein sequence suggesting that reassortment might be a possible explanation. Conclusion Our findings suggest that the Greek strains represent an example of "frozen evolution" and probably reassortment between genetically distinct co-circulated strains. Therefore expanding current equine influenza surveillance efforts is a necessity.

Published
2011
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19. Validation of a Loop-Mediated Amplification/ISO 6579-Based Method for Analysing Soya Meal for the Presence of Salmonella enterica.

Author

D'Agostino, Martin, Robles, Susana, Hansen, Flemming, Ntafis, Vasileios, Ikonomopoulos, John, Kokkinos, Petros, Alvarez-Ordonez, Avelino, Jordan, Kieran, Delibato, Elisabetta, Kukier, Elżbieta, Sieradzki, Zbigniew, Kwiatek, Krzysztof, Milanov, Dubravka, Petrović, Tamaš, Gonzalez-Garcia, Patricia, Lazaro, David, Jackson, Emily, Forsythe, Stephen, O'Brien, Lorna, and Cook, Nigel

Abstract

An alternative method for detection of Salmonella spp. in animal feed, based on the use of loop-mediated amplification (LAMP) in conjunction with a standard culturing procedure, was compared with the standard ISO 6579 as reference method, using soya meal as the test matrix. In the method comparison study, the sensitivities for both the alternative and reference methods were 100 %. The relative level of detection was 1.000. Tested against 100 Salmonella and 30 non- Salmonella strains, the LAMP-based method was 99 % inclusive and 100 % exclusive. The interlaboratory trial involved ten laboratories from eight European countries, testing eight samples at three contamination levels: 0 cfu/100 g, 1-5 cfu/100 g and 14-68 cfu/100 g. The trial specificity, or percentage correct identification of uncontaminated samples, was 96.3 % for both the reference methods and the LAMP/ISO 6579 alternative method, thus demonstrating its suitability for adoption as a procedure for rapid identification of Salmonella uncontaminated soya meal. [ABSTRACT FROM AUTHOR]

Published
2016
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21. European Surveillance for Pantropic Canine Coronavirus

Author

Decaro, Nicola, primary, Cordonnier, Nathalie, additional, Demeter, Zoltan, additional, Egberink, Herman, additional, Elia, Gabriella, additional, Grellet, Aurélien, additional, Le Poder, Sophie, additional, Mari, Viviana, additional, Martella, Vito, additional, Ntafis, Vasileios, additional, von Reitzenstein, Marcela, additional, Rottier, Peter J., additional, Rusvai, Miklos, additional, Shields, Shelly, additional, Xylouri, Eftychia, additional, Xu, Zach, additional, and Buonavoglia, Canio, additional

Published
2013
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