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Detection and conversion of mechanical forces into biochemical signals is known as mechanotransduction. From cells to tissues, mechanotransduction regulates migration, proliferation, and differentiation in processes such as immune responses, development, and cancer progression. Mechanosensitive structures such as integrin adhesions, the actin cortex, ion channels, caveolae, and the nucleus sense and transmit forces. In vitro approaches showed that mechanosensing is based on force-dependent protein deformations and reorganizations. However, the mechanisms in cells remained unclear since cell imaging techniques lacked molecular resolution. Thanks to recent developments in super-resolution microscopy (SRM) and molecular force sensors, it is possible to obtain molecular insight of mechanosensing in live cells. We discuss how understanding of molecular mechanotransduction was revolutionized by these innovative approaches, focusing on integrin adhesions, actin structures, and the plasma membrane. Mechanotransduction is involved in a variety of cell and tissue processes. For this, mechanosensitive structures, such as integrin adhesions, the cytoskeleton, or the plasma membrane, are key to sense, integrate, and transmit mechanical forces. Novel imaging methods and molecular sensors have unveiled how molecular mechanosensing occurs in these structures within the context of live cells. These include super-resolution imaging, single particle tracking, and molecular force sensors. Super-resolution microscopy and single protein tracking have revealed the 3D nanoscale organization and dynamics of integrin adhesion structures in various cell types, which are divided into functional nanolayers with specific paths for protein diffusion. Forces on actin regulators and F-actin trigger conformational changes controlling actin regulator function and binding, and therefore F-actin assembly and architecture. In migrating cells, mechanical plasticity emerges from global geometrical reorganization of actin networks under loads, or forces from elongating actin filaments controlling locally actin regulators dynamics and functions. Cell surface mechanics are emerging as a key parameter controlling cell motility, division, and differentiation. Variations in cortical tension and membrane-to-cortex attachment regulate the timing of differentiation while the nanoscale architecture of the actin cortex is linked to cortical tension. [ABSTRACT FROM AUTHOR]

The literature suggests that children from nuclear families tend to show a more secure attachment than children from single parent or step families. The goals of this study are as follows: investigate the quality of child's attachment behaviour across nuclear, single parent and step families, evaluate the convergence between mother and their child's perceptions on the quality of mother-child attachment behaviour. This is a cross-sectional study, whose sample consisted of 168 children 8-11 years-old (M=9.17) and their mothers. The research protocol included the Separation Anxiety Test (SAT) and the Maternal Perception Scale of Children S Attachment Behaviour (PCV-M). Results show that there is no significant convergence between the maternal perception of their child's attachment behaviour and the child's attachment representation. Nonetheless, in single parent families significant differences were found in the PCV-M secure base behaviour dimension, depending on the representation of children's attachment as secure or insecure. There were no association between type of family and children's attachment representation neither differences in the maternal perception of attachment between the different types of family. In conclusion, the mother's perception of their child's attachment behaviour doesn't vary across different types of families, even if there is a low degree of convergence between mothers and their offspring regarding the quality of children's attachment behaviour. [ABSTRACT FROM AUTHOR]