1. A dual function for Pex3p in peroxisome formation and inheritance
- Author
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Munck, Joanne M., Motley, Alison M., Nuttall, James M., and Hettema, Ewald H.
- Subjects
Endoplasmic reticulum -- Physiological aspects ,Endoplasmic reticulum -- Genetic aspects ,Peroxisomes -- Physiological aspects ,Peroxisomes -- Genetic aspects ,Brewer's yeast -- Physiological aspects ,Brewer's yeast -- Genetic aspects ,Biological sciences - Abstract
Saccharomyces cerevisiae Pex3p has been shown to act at the ER during de novo peroxisome formation. However, its steady state is at the peroxisomal membrane, where its role is debated. Here we show that Pex3p has a dual function: one in peroxisome formation and one in peroxisome segregation. We show that the peroxisome retention factor Inp 1p interacts physically with Pex3p in vitro and in vivo, and split-GFP analysis shows that the site of interaction is the peroxisomal membrane. Furthermore, we have generated PEX3 alleles that support peroxisome formation but fail to support recruitment of Inp 1p to peroxisomes, and as a consequence are affected in peroxisome segregation. We conclude that Pex3p functions as an anchor for Inp1 p at the peroxisomal membrane, and that this function is independent of its role at the ER in peroxisome biogenesis. doi 10.1083/jcb.200906161
- Published
- 2009