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The Human Anatomy & Physiology Society (HAPS) has published a set of learning outcomes to be used for curriculum design of a one-semester introductory physiology course. The outcomes are competency-based and are mapped to core concepts and skills students should develop as undergraduates. This paper is an introduction to how the physiology learning outcomes are organized and how they can be used.

Abstract Background The purpose of this study was to identify and describe common views of people with lived experience of mental health challenges – consumers and carers, families and supporters – of what they consider the most important measures to include in health economic evaluations which assess the incremental value of competing options in mental health care. Methods Participants (n = 111) were people living in the state of Victoria, Australia, who identified as consumers of mental healthcare (n = 38); carers, family members and/or supporters (n = 43); or both (n = 30). Factor analysis based on Q-Methodology was used to identify clusters of people who hold similar viewpoints. Common viewpoints were described in terms of the characteristics of the group, and a qualitative interpretation was conducted on the basis of distinguishing statements and quotes provided in participants’ own words. Results We identified four common views: (1) safety before all else, prioritizing physical, sexual and psychological safety; (2) hope and partnership in processes of care; (3) physical and emotional health and wellbeing; and (4) care access, continuity and partnership with families. Although different priorities were identified for each viewpoint, key priority areas that were common to all views were having an environment in the health service that fosters respect and dignity, and that consumers feel heard and listened to. In sub-group and qualitative analyses, differences were observed regarding the likelihood of consumers and carers holding each of the views, as well as by age group. Conclusions While some differences were noted between the views of consumers and carers and different age groups, there was also common ground regarding what outcomes are of most importance to measure. Including these measures in evaluation frameworks would provide a way of focussing mental healthcare decisions on the aspects of mental healthcare that are of most value to consumers and carers, thereby addressing an important shortcoming of current approaches to decision-making in mental healthcare.

Abstract Background Regular exercise can mitigate side effects of cancer treatment. However, only a small proportion of adults with cancer meet exercise guidelines, and older adults (> 65 years) are underrepresented in cancer rehabilitation research. Peer support facilitates health-promoting behaviours in general populations, but interventions merging exercise and peer support for older adults with cancer are not examined. The purpose of this study was to determine the feasibility and preliminary effectiveness of a virtual partner-based peer support exercise intervention for older adult female cancer survivors. Methods Older adult female cancer survivors with internet access and currently participating in

Abstract Background Genetic perturbation screens with single-cell readouts have enabled rich phenotyping of gene function and regulatory networks. These approaches have been challenging in vivo, especially in adult disease models such as cancer, which include mixtures of malignant and microenvironment cells. Glioblastoma (GBM) is a fatal cancer, and methods of systematically interrogating gene function and therapeutic targets in vivo, especially in combination with standard of care treatment such as radiotherapy, are lacking. Results Here, we iteratively develop a multiplex in vivo perturb-seq CRISPRi platform for single-cell genetic screens in cancer and tumor microenvironment cells that leverages intracranial convection enhanced delivery of sgRNA libraries into mouse models of GBM. Our platform enables potent silencing of drivers of in vivo growth and tumor maintenance as well as genes that sensitize GBM to radiotherapy. We find radiotherapy rewires transcriptional responses to genetic perturbations in an in vivo-dependent manner, revealing heterogenous patterns of treatment sensitization or resistance in GBM. Furthermore, we demonstrate targeting of genes that function in the tumor microenvironment, enabling alterations of ligand-receptor interactions between immune and stromal cells following in vivo CRISPRi perturbations that can affect tumor cell phagocytosis. Conclusion In sum, we demonstrate the utility of multiplexed perturb-seq for in vivo single-cell dissection of adult cancer and normal tissue biology across multiple cell types in the context of therapeutic intervention, a platform with potential for broad application.

A new species of the genus Chrysonotomyia Ashmead, Chrysonotomyia susbelli sp. nov., is described from the Rice University campus in Houston, Texas, USA. The species is a parasitoid emerging from Neuroterus nr. bussae galls in leaves of the southern live oak (Quercus virginiana). This represents the 6th species described from North America north of Mexico and the first in the world known to parasitize cynipid gall wasps. This discovery hints at an entire undiscovered niche between Chrysonotomyia parasitoids, cynipid gall wasps, and oaks in the Nearctic, which is a global biodiversity hotspot for oaks and cynipids. This new species description is complemented by mtDNA-COI-barcode data and information on the natural history of this species. We record host association, phenology, and report a leaf-scanning behavior performed by females, presumably to search for host galls. Modifications to the key of New World members of the genus (Hansson 2004) are included to integrate this new species.

Abstract Background Intensive deployment of insecticide based malaria vector control tools resulted in the rapid evolution of phenotypes resistant to these chemicals. Understanding this process at the genomic level is important for the deployment of successful vector control interventions. Therefore, longitudinal sampling followed by whole genome sequencing (WGS) is necessary to understand how these evolutionary processes evolve over time. This study investigated the change in genetic structure and the evolution of the insecticide resistance variants in natural populations of Anopheles gambiae over time and space from 2012 to 2017 in Burkina Faso. Methods New genomic data have been generated from An. gambiae mosquitoes collected from three villages in the western part of Burkina Faso between 2012 and 2017. The samples were whole-genome sequenced and the data used in the An. gambiae 1000 genomes (Ag1000G) project as part of the Vector Observatory. Genomic data were analysed using the analysis pipeline previously designed by the Ag1000G project. Results The results showed similar and consistent nucleotide diversity and negative Tajima’s D between An. gambiae sensu stricto (s.s.) and Anopheles coluzzii. Principal component analysis (PCA) and the fixation index (F ST ) showed a clear genetic structure in the An. gambiae sensu lato (s.l.) species. Genome-wide F ST and H12 scans identified genomic regions under divergent selection that may have implications in the adaptation to ecological changes. Novel voltage-gated sodium channel pyrethroid resistance target-site alleles (V402L, I1527T) were identified at increasing frequencies alongside the established alleles (Vgsc-L995F, Vgsc-L995S and N1570Y) within the An. gambiae s.l. populations. Organophosphate metabolic resistance markers were also identified, at increasing frequencies, within the An. gambiae s.s. populations from 2012 to 2017, including the SNP Ace1-G280S and its associated duplication. Variants simultaneously identified in the same vector populations raise concerns about the long-term efficacy of new generation bed nets and the recently organophosphate pirimiphos-methyl indoor residual spraying in Burkina Faso. Conclusion These findings highlighted the benefit of genomic surveillance of malaria vectors for the detection of new insecticide resistance variants, the monitoring of the existing resistance variants, and also to get insights into the evolutionary processes driving insecticide resistance.

Abstract Background Young adults face unique vulnerabilities during major life disruptions like the COVID-19 pandemic. The pandemic contributed to increases in mental health challenges and substance use among young adults. This study explores the experiences of young adults who increased their cannabis use during the pandemic. Methods Participants were recruited from the Nicotine Dependence in Teens (NDIT) study, and qualitative data were collected through semi-structured interviews conducted via Zoom. A total of 25 participants (ages 33–34) reporting increased cannabis use during the pandemic were included. Thematic analysis and gender-based analysis was employed to extract key themes. Results Five themes emerged: (1) No disruption in cannabis use; (2) Cannabis use to manage declines in mental health; (3) Cannabis use to break up pandemic boredom; (4) Cannabis use as an expression of freedom; (5) Cannabis use as “another way to chill out.” Conclusions This research provides valuable perspectives on how major life disruptions, like the COVID-19 pandemic, influence cannabis use among young adults. The findings offer guidance for public health initiatives and highlight avenues for further investigation.

Background. Monitoring food intake and physical activity (PA) using tracking applications may support behavior change. However, few longitudinal studies identify the characteristics of young adults who track their behavior, findings that could be useful in designing tracking-related interventions. Our objective was to identify predictors of past-year food and PA tracking among young adults. Methods. Data were available for 676 young adults participating in the ongoing longitudinal Nicotine Dependence in Teens Study. Potential predictors were measured in 2017-2020 at age 31, and past-year food and PA tracking were measured in 2021-2022 at age 34. Each potential predictor was studied in a separate multivariable logistic regression model controlling for age, sex, and educational attainment. Results. One third (37%) of participants reported past-year PA tracking; 14% reported past-year food, and 10% reported both. Nine and 11 of 41 potential predictors were associated with food and PA tracking, respectively. Compensatory behaviors after overeating, trying to lose weight, self-report overweight, reporting a wide variety of exercise behaviors, and pressure to lose weight predicted both food and PA tracking. Conclusion. Food and PA tracking are relatively common among young adults. If the associations observed herein between compensatory behavior after overeating and tracking (among other observed associations) are replicated and found to be causal, caution may need to be exercised in making "blanket" recommendations to track food intake and/or PA to all young adults seeking behavior change.

Recent trends of migration have contributed to the growing number of Students with Limited or Interrupted Formal Education (SLIFE) in our K-12 classrooms in the United States and worldwide. Simultaneously, the visibility of SLIFE in academic publications from around the world (i.e., Custodio & O'Loughlin, 2017; DeCapua et al., 2020; Pentón Herrera, 2022) contributes to a general understanding of best practices on what this population needs and how teachers can best help them. In this article, we rely on recent and relevant available publications, as well as personal experiences, to recommend essential "social-emotional" and "academic" considerations that teachers and stakeholders should know to support SLIFE effectively. The social-emotional considerations shared include social and emotional needs, cultural dissonance, connections to programming and services, and interpersonal relationships. The academic considerations shared are programming, first language (L1) literacy instruction, appropriate scaffolding, oscillating target language (L2) literacy instruction, and authentic learning beyond the school walls.

Learning outcomes are an essential element in curriculum development because they describe what students should be able to do by the end of a course or program and they provide a roadmap for designing assessments. This article describes the development of competency-based learning outcomes for a one-semester undergraduate introductory human physiology course. Key elements in the development process included decisions about terminology, eponyms, use of the word "normal," and similar considerations for inclusivity. The outcomes are keyed to related physiology core concepts and to process skills that can be taught along with the content. The learning outcomes have been published under a Creative Commons license by the Human Anatomy and Physiology Society (HAPS) and are available free of charge on the HAPS website.

Background: The context in which school-based health-promoting interventions are implemented is key for the delivery and success of these interventions. However, little is known about whether school culture differs by school deprivation. Methods: Using data from PromeSS, a cross-sectional study of 161 elementary schools in Québec, Canada, we drew from the Health Promoting Schools theoretical framework to develop four measures of health-promoting school culture (i.e., school physical environment, school/teacher commitment to student health, parent/community engagement with the school, ease of principal leadership) using exploratory factor analysis. One-way ANOVA with post-hoc Tukey-Kramer analyses was used to examine associations between each measure and social and material deprivation in the school neighborhood. Results: Factor loadings supported the content of the school culture measures and Cronbach's alpha indicated good reliability (range: 0.68-0.77). As social deprivation in the school neighborhood increased, scores for both school/teacher commitment to student health and parent/community engagement with the school decreased. Implications for School Health Policy, Practice, and Equity: Implementation of health-promoting interventions in schools located in socially deprived neighborhoods may require adapted strategies to address challenges related to staff commitment and parental and community involvement. Conclusion: The measures developed herein can be used to investigate school culture and interventions for health equity.

Abstract Myosin motors perform many fundamental functions in eukaryotic cells by providing force generation, transport or tethering capacity. Motor activity control within the cell involves on/off switches, however, few examples are known of how myosins regulate speed or processivity and fine-tune their activity to a specific cellular task. Here, we describe a phosphorylation event for myosins of class VI (MYO6) in the motor domain, which accelerates its ATPase activity leading to a 4-fold increase in motor speed determined by actin-gliding assays, single molecule mechanics and stopped flow kinetics. We demonstrate that the serine/threonine kinase DYRK2 phosphorylates MYO6 at S267 in vitro. Single-molecule optical-tweezers studies at low load reveal that S267-phosphorylation results in faster nucleotide-exchange kinetics without change in the working stroke of the motor. The selective increase in stiffness of the acto-MYO6 complex when proceeding load-dependently into the nucleotide-free rigor state demonstrates that S267-phosphorylation turns MYO6 into a stronger motor. Finally, molecular dynamic simulations of the nucleotide-free motor reveal an alternative interaction network within insert-1 upon phosphorylation, suggesting a molecular mechanism, which regulates insert-1 positioning, turning the S267-phosphorylated MYO6 into a faster motor.

IntroductionLong-term availability of health-promoting interventions (HPIs) in school settings can translate into health benefits for children. However, little is known about factors associated with HPI institutionalization in schools. In this study, we identified correlates of the institutionalization of HPIs offered in elementary schools in Quebec, Canada. MethodsIn two-part, structured telephone interviews over three academic years (2016–2019), elementary school principals (or their designees) throughout Quebec identified an index HPI offered at least once in their school during the previous three years, and were asked whether it was institutionalized (i.e. explicitly written in the school’s educational project, e.g. in the form of educational objectives and means of achieving them). We examined associations between institutionalization and 10 school-related and 16 HPI-related characteristics in univariable and multivariable logistic regression analyses. ResultsSchool key informants (n = 163) reported on 147 different HPIs that had been available in their schools in the past three years, 56% of which were institutionalized. Three aspects of school culture—parent/community engagement with the school, school/teacher commitment to student health and school physical environment—were positively associated with HPI institutionalization. HPI-related characteristics positively associated with HPI institutionalization included number of competencies addressed by the HPI, number of teaching strategies employed, modifications made to the HPI prior to or during implementation and perceived success of the HPI. Inviting families or community groups to participate in the HPI was inversely associated with institutionalization. ConclusionBetter understanding of factors associated with HPI institutionalization may inform the development of school-based HPIs that have the potential for sustainability.

IntroductionL’offre à long terme d’interventions de promotion de la santé (IPS) en milieu scolaire peut se révéler bénéfique pour la santé des enfants. Toutefois, on en sait peu sur les facteurs associés à cette institutionnalisation dans les écoles. Dans cette étude, nous avons dégagé les corrélats de l’institutionnalisation des IPS offertes dans les écoles primaires du Québec (Canada). MéthodologieDans le cadre d’entrevues téléphoniques structurées en deux parties réalisées sur trois années scolaires (2016-2019), des directeurs et directrices d’écoles primaires (ou leurs représentants désignés) du Québec devaient choisir une IPS de référence ayant été offerte au moins une fois dans leur école dans les trois années précédentes. Ils devaient ensuite indiquer si l’IPS avait été institutionnalisée (c.-à-d. si elle figurait explicitement dans le projet éducatif de l’école, par exemple sous la forme d’objectifs éducatifs accompagnés des mesures permettant leur atteinte). Nous avons analysé les associations entre, d’une part, l’institutionnalisation des IPS et, d’autre part, dix caractéristiques liées aux écoles et seize caractéristiques liées aux IPS à l’aide d’analyses de régression logistique univariées et multivariées. RésultatsLes informateurs clés des écoles (n = 163) ont fait état de 147 IPS différentes ayant été offertes dans leur école dans les trois années précédentes et 56 % de ces IPS avaient été institutionnalisées. Trois dimensions de la culture de l’école – soit 1) la participation des parents et de la collectivité à la vie scolaire, 2) l’engagement de l’école et du personnel enseignant à l’égard de la santé des élèves et 3) l’environnement physique de l’école – étaient associées positivement à l’institutionnalisation des IPS. En ce qui a trait aux caractéristiques liées aux IPS, ce sont le nombre de compétences visées par celles-ci, le nombre de stratégies d’enseignement utilisées, les modifications apportées à l’IPS avant ou pendant sa mise en oeuvre et le succès perçu de l’IPS qui ont présenté une association positive avec l’institutionnalisation des IPS. Le fait d’inviter les familles ou les groupes communautaires à participer à l’IPS était inversement associé à l’institutionnalisation. ConclusionUne meilleure compréhension des facteurs liés à l’institutionnalisation des IPS pourrait éclairer l’élaboration d’IPS présentant un potentiel de durabilité en milieu scolaire.

Background: We evaluated a transition to retirement intervention that adapted strategies used to support employment of people with intellectual disability in mainstream workplaces. The intervention facilitated their independent participation in mainstream community groups and volunteering. Methods: We randomised 29 older Australians who currently/previously worked in mainstream employment into an intervention group or wait-list control group. Hours of independent participation in target activities were collected before and after the 13-week initial intervention/waiting period, and at 52 weeks for intervention participants. Results: Six of the 12 intervention participants selected an activity and increased hours of independent participation following 13 weeks in the Keeping Active (KA) program and eight were independently accessing their selected activity at 52-week follow-up. None of the 19 wait-list participants increased their independent participation after 13 weeks. Conclusions: The intervention was effective and highlighted the need to account for factors such as participant choice in evaluation of individualised programs.

ABSTRACTBackground Chronic pain and higher body weight frequently co-occur. This common comorbidity is thought to be mediated by the use of comfort eating as a strategy for managing both the physical and psychological pain and discomfort associated with flare-ups of chronic pain. Valid and reliable assessment tools are needed to inform the development of effective treatments.Aims This study aimed to assess the psychometric properties of a new brief measure of pain-induced comfort eating in chronic pain, the Pain-Induced Comfort Eating Scale (PICES).Methods A sample of 166 patients with chronic pain completed an online test battery including the PICES along with measures of chronic pain and pain-related symptoms, disordered eating, and related psychological factors.Results Results of exploratory factor analysis revealed a single-factor model for the four-item PICES. Further, the PICES demonstrated evidence of good internal consistency as well as convergent validity with demonstrated correlations with related measures. The results of this study also revealed that comfort eating in chronic pain appears to be related to psychological distress; the PICES correlated more strongly with measures assessing mood and psychological distress compared to interference/intensity of physical pain itself. Scores on the PICES also correlated strongly with measures of uncontrolled and emotional eating.Conclusions Overall, our results indicate that the PICES provides a valid and useful brief measure of comfort eating in chronic pain that might be useful to inform treatments targeting the comorbid disordered eating practices that can lead to higher body weights in patients with chronic pain.

The search for cell-permeable drugs has conventionally focused on low-molecular weight (MW), nonpolar, rigid chemical structures. However, emerging therapeutic strategies break traditional drug design rules by employing flexibly linked chemical entities composed of more than one ligand. Using complementary genome-scale chemical-genetic approaches we identified an endogenous chemical uptake pathway involving interferon-induced transmembrane proteins (IFITMs) that modulates the cell permeability of a prototypical biopic inhibitor of MTOR (RapaLink-1, MW: 1784 g/mol). We devised additional linked inhibitors targeting BCR-ABL1 (DasatiLink-1, MW: 1518 g/mol) and EIF4A1 (BisRoc-1, MW: 1466 g/mol), uptake of which was facilitated by IFITMs. We also found that IFITMs moderately assisted some proteolysis-targeting chimeras and examined the physicochemical requirements for involvement of this uptake pathway.

Abstract Background Mental health conditions represent one of the major groups of non-transmissible diseases. Physical activity (PA) and sedentary time (ST) have been shown to affect mental health outcomes in opposite directions. In this study, we use accelerometery-derived measures of PA and ST from the UK Biobank (UKB) and depression, anxiety and well-being data from the UKB mental health questionnaire as well as published summary statistics to explore the causal associations between these phenotypes. Methods We used MRlap to test if objectively measured PA and ST associate with mental health outcomes using UKB data and summary statistics from published genome-wide association studies. We also tested for bidirectional associations. We performed sex stratified as well as sensitivity analyses. Results Genetically instrumented higher PA was associated with lower odds of depression (OR = 0.92; 95% CI: 0.88, 0.97) and depression severity (beta = − 0.11; 95% CI: − 0.18, − 0.04), Genetically instrumented higher ST was associated higher odds of anxiety (OR = 2.59; 95% CI: 1.10, 4.60). PA was associated with higher well-being (beta = 0.11, 95% CI: 0.04; 0.18) and ST with lower well-being (beta = − 0.18; 95% CI: − 0.32, − 0.03). Similar findings were observed when stratifying by sex. There was evidence for a bidirectional relationship, with higher genetic liability to depression associated with lower PA (beta = − 0.25, 95% CI: − 0.42; − 0.08) and higher well-being associated with higher PA (beta = 0.15; 95% CI: 0.05, 0.25). Conclusions We have demonstrated the bidirectional effects of both PA and ST on a range of mental health outcomes using objectively measured predictors and MR methods for causal inference. Our findings support a causal role for PA and ST in the development of mental health problems and in affecting well-being.

Due to the changing climate and human activity, more and more researchers started to focus on non-stationarity in hydrology. In the Wei River Basin, which is the largest tributary of the Yellow River, there is a significant reduction in the total amount of water resources which has been found in past decades. Additionally, the distribution of water resources within the basin is unbalanced, with the lower reaches and southern regions having relatively abundant water resources and other regions lacking these resources. Within this situation, it is important to consider the spatial aspect of water resource management. Four non-stationarity detection methods have been applied to investigate variation in seasonal discharge series. Two meteorological factors have also been analyzed. Based on test results and Köppen Geiger Climate classification, the water resource management has been investigated spatially. As for results, the Baojixia Channel has significant impact on the abrupt change of discharge, while the precipitation and temperature may have an impact on the discharge trend change. In addition, there was no clear evidence to prove that the climate zones impact spatially on the non-stationarity of discharge.

IntroductionAlthough body weight has been positioned as a strong predictor of physical and mental health, positive and negative body-related psychosocial factors may also be important. Further, both theoretical tenets and empirical evidence suggest that these associations may differ by gender. Our objectives were to examine the associations between body-related self-conscious emotions (body shame, body authentic pride) and physical and mental health in young adults, and to identify potential differences in these associations by gender. MethodsData for this cross-sectional study were drawn from the Nicotine Dependence in Teens (NDIT) study for 799 young adults (M [SD] age = 33.6 y [0.5]; 43.9% male). We estimated the associations between each of body shame and body authentic pride (i.e. the exposures) and both self-rated physical and self-rated mental health (i.e. the outcomes) in linear regression models that controlled for age, education and body mass index, and we examined potential gender differences in these associations by conducting gender-stratified analyses. ResultsIn females, self-rated health and mental health decreased by 0.37 and 0.38, respectively, with each unit increase in body shame. Self-rated health and mental health increased by 0.25 and 0.23, respectively, for each unit increase in body authentic pride. In males, self-rated health and mental health decreased by 0.35 and 0.45, respectively, with each unit increase in body shame, and increased by 0.32 and 0.21, respectively, with each unit increase in body authentic pride. ConclusionInterventions that focus on body weight to the exclusion of body-related self-conscious emotions may side-step a key contributor to self-rated health.

IntroductionS’il est vrai que le poids constitue un fort prédicteur de la santé physique et mentale, il n’en demeure pas moins que les facteurs psychosociaux positifs et négatifs en lien avec le corps sont également susceptibles de jouer un rôle important. En outre, tant des fondements théoriques que des données empiriques tendent à indiquer que ces associations peuvent varier en fonction du genre. Nos objectifs étaient, d’une part, d’analyser les associations entre les émotions liées à la conscience de son corps (honte corporelle, fierté corporelle authentique) et la santé physique et mentale chez les jeunes adultes et, d’autre part, de cerner les différences potentielles dans ces associations en fonction du genre. MéthodologieLes données utilisées dans cette étude transversale ont été tirées de l’étude sur la dépendance à la nicotine chez les adolescents (étude NICO) et portaient sur 799 jeunes adultes (âge moyen = 33,6 ans [écart-type = 0,5]; 43,9 % d’hommes). Nous avons estimé les associations entre, d’une part, la honte corporelle et la fierté corporelle authentique (variables d’exposition) et, d’autre part, l’état de santé physique autoévalué et l’état de santé mentale autoévalué (variables de résultat) dans des modèles de régression linéaire tenant compte de l’âge, du niveau de scolarité et de l’indice de masse corporelle, et nous avons étudié les différences potentielles dans ces associations en fonction du genre en effectuant des analyses stratifiées selon le genre. RésultatsChez les femmes, l’état de santé physique autoévalué et l’état de santé mentale autoévalué diminuaient respectivement de 0,37 et de 0,38 pour chaque augmentation d’une unité de la honte corporelle. L’état de santé physique autoévalué et l’état de santé mentale autoévalué augmentaient de respectivement 0,25 et de 0,23 pour chaque augmentation d’une unité de fierté corporelle authentique. Chez les hommes, l’état de santé physique autoévalué et l’état de santé mentale autoévalué diminuaient de respectivement 0,35 et de 0,45 pour chaque augmentation d’une unité de la honte corporelle. L’état de santé physique autoévalué et l’état de santé mentale autoévalué augmentaient de respectivement 0,32 et de 0,21 pour chaque augmentation d’une unité de la fierté corporelle authentique. ConclusionLes interventions qui ciblent le poids sans tenir compte des émotions liées à la conscience du corps sont susceptibles d’omettre un facteur clé de l’état de santé autoévalué.