Search

Abstract The prognoses of patients who undergo open spinal endoscopy (OSE) decompression significantly differ by scoliosis type and symptom despite the use of uniform standards and procedures for the decompression surgery. These differences may be directly related to the selection and formulation of surgical strategies but their cause remains unclear. The aim of this study was to verify and evaluate the efficacy of the "Symptom, Stenosis and Segment classification (SSS classification)" in determining an appropriate surgical strategy and to analyze the differences in the outcomes of different patients after receiving the selected surgical strategy. The results of this study ultimately provide a theoretical basis for the specific optimization of surgical strategies guided by the "SSS classification". This work was a retrospective study. We reviewed 55 patients with scoliosis and spinal stenosis who underwent "pear-shaped" decompression under OSE from May 2021 to June 2023 treated by our surgical team. To classify different types of patients, we defined the "SSS classification" system. The permutation and combination of subtypes in Symptom (including three subtypes: Convex = v, Concave = c and Bilateral = b), Stenosis (including three subtypes: Convex = v, Concave = c and Bilateral = b), and Segment (including two subtypes: Edge = e and Inside = i) yields 18 possible types (details in Table 1) in this classification system. To classify different types of surgeries, we also defined the operation system. The VAS Back and VAS Leg scores after surgical treatment were significantly lower in all patients 3 months after surgery than before surgery. (**P

The prognoses of patients who undergo open spinal endoscopy (OSE) decompression significantly differ by scoliosis type and symptom despite the use of uniform standards and procedures for the decompression surgery. These differences may be directly related to the selection and formulation of surgical strategies but their cause remains unclear. The aim of this study was to verify and evaluate the efficacy of the "Symptom, Stenosis and Segment classification (SSS classification)" in determining an appropriate surgical strategy and to analyze the differences in the outcomes of different patients after receiving the selected surgical strategy. The results of this study ultimately provide a theoretical basis for the specific optimization of surgical strategies guided by the "SSS classification". This work was a retrospective study. We reviewed 55 patients with scoliosis and spinal stenosis who underwent "pear-shaped" decompression under OSE from May 2021 to June 2023 treated by our surgical team. To classify different types of patients, we defined the "SSS classification" system. The permutation and combination of subtypes in Symptom (including three subtypes: Convex = v, Concave = c and Bilateral = b), Stenosis (including three subtypes: Convex = v, Concave = c and Bilateral = b), and Segment (including two subtypes: Edge = e and Inside = i) yields 18 possible types (details in Table 1) in this classification system. To classify different types of surgeries, we also defined the operation system. The VAS Back and VAS Leg scores after surgical treatment were significantly lower in all patients 3 months after surgery than before surgery. (**P < 0.05). The Svve type accounted for the greatest proportion of patients (62.50%) in the VAS back remission group, and the Scce type accounted for the greatest proportion (57.14%) in the VAS back ineffective group. According to the VAS leg score, the percentage of patients in whom Svve was detected in the VAS leg remission group reached 60.87%, and the percentage of patients in whom Svve was detected in the VAS leg ineffective group reached 44.44%. Svve accounted for the greatest proportion of cases (61.22%) in the JOA-effective group, and Scce accounted for the greatest proportion of cases (50.00%) in the JOA-ineffective group. In the JOA-effective group, the Ovv type accounted for the greatest proportion (up to 79.59%), while in the JOA-ineffective group, Occ and Ovv accounted for 50.00% of the cases each. The proportions of Svve type were the highest in the healthy group (up to 60.00%) and the ODI-effective group (up to 50.00%). The Ovv type accounted for the greatest proportion of patients in the ODI-effective group (up to 80.00%), and the Occ type accounted for the greatest proportion of patients in the ODI-ineffective group (up to 60.00%). Most of the surgical plans formulated by the "SSS classification" method were considered appropriate, and only when the symptoms of patients were located on the concave side did the endoscopic decompression plan used in the present study have a limited ability to alleviate symptoms. [ABSTRACT FROM AUTHOR]

“Synergistic Observing Network for Ocean Prediction (SynObs)” was launched in 2022 as a project of the United Nations Decade of Ocean Science for Sustainable Development to evaluate the importance of ocean observation systems and co-design the future evolution of the ocean observing network. SynObs is currently leading the flagship OSEs/OSSEs, an internationally coordinated activity in which observing system experiments (OSEs) and observing system simulation experiments (OSSEs) are conducted using a variety of ocean and coupled atmosphere–ocean prediction systems to evaluate ocean observation impacts consistent across most prediction systems. The flagship OSEs/OSSEs comprises the ocean prediction (OP) OSEs for high-resolution ocean predictions, the subseasonal-to-seasonal (S2S) OSEs for long-term lead-time coupled ocean–atmosphere predictions, and the OP OSSEs for evaluating new and future observing systems. SynObs plans to use the results of the flagship OSEs to contribute to the reports on the ocean observing network design made by international organizations and projects. Here, we introduce this initiative, and we report on some initial results. Some observation impacts consistent across four ocean prediction systems are found by a preliminary analysis of the analysis runs for the OP OSEs. For example, impacts of the altimetry data on the assimilated sea surface height (SSH) field are generally large in the westerly boundary current regions and around Antarctic Circumpolar Currents where SSH has large variability but are small in the tropical regions, despite the relatively large SSH variability there. The analysis also indicates the possibility that there are some characteristic differences in the observation impacts between low-resolution and eddy-resolving ocean prediction systems. Although OSE outputs of only four ocean prediction systems are available now, we will make further investigation, adding OSE outputs of other prediction systems that will be submitted in the near future.

Background: Glatiramer acetate (GA) is a well-established treatment option for patients with clinically isolated syndrome and relapsing–remitting multiple sclerosis (MS) with few side effects. The double transgenic mouse model spontaneous opticospinal encephalomyelitis (OSE), based on recombinant myelin oligodendrocyte glycoprotein35-55 reactive T and B cells, mimicks features of chronic inflammation and degeneration in MS and related disorders. Here, we investigated the effects of prophylactic GA treatment on the clinical course, histological alterations and peripheral immune cells in OSE. Objective: To investigate the effects of prophylactic glatiramer acetate (GA) treatment in a mouse model of spontaneous opticospinal encephalomyelitis (OSE). Methods: OSE mice with a postnatal age of 21 to 28 days without signs of encephalomyelitis were treated once daily either with 150 µg GA or vehicle intraperitoneally (i. p.). The animals were scored daily regarding clinical signs and weight. The animals were sacrificed after 30 days of treatment or after having reached a score of 7.0 due to animal care guidelines. We performed immunohistochemistry of spinal cord sections and flow cytometry analysis of immune cells. Results: Preventive treatment with 150 µg GA i. p. once daily significantly reduced clinical disease progression with a mean score of 3.9 ± 1.0 compared to 6.2 ± 0.7 in control animals (p < 0.01) after 30 d in accordance with positive effects on weight (p < 0.001). The immunohistochemistry showed that general inflammation, demyelination or CD11c+ dendritic cell infiltration did not differ. There was, however, a modest reduction of the Iba1+ area (p < 0.05) and F4/80+ area upon GA treatment (p < 0.05). The immune cell composition of secondary lymphoid organs showed a trend towards an upregulation of regulatory T cells, which lacked significance. Conclusions: Preventive treatment with GA reduces disease progression in OSE in line with modest effects on microglia/macrophages. Due to the lack of established prophylactic treatment options for chronic autoimmune diseases with a high risk of disability, our study could provide valuable indications for translational medicine. [ABSTRACT FROM AUTHOR]

Ernst Papanek (1900-1973) was an Austrian pedagogue who, between 1938 and 1940, was responsible for children's homes in France he directed for the OSE (OEuvre de secours aux en-fants). In these children's homes at Montmorency, close to Paris, Papanek tried to treat the traumatized children who had lost their homes, their parents, and those who struggled with their own identities that had been called into question by ideological propaganda and personal experiences alike. Papanek decided to give their souls a new home and not only intended to keep them physically alive but also to use a new form of therapy that was supposed to treat the children as a group. In 1940, Papanek had to escape from France, via Spain and Portugal, to the United States, and he tried to rescue the children by bringing them across the Atlantic as well. However, when he advocated for the idea to continue their treatment as a group in a children's home like the ones he had run in France, he met with resistance, as such approaches were uncommon in the United States, where social workers sought to separate the children and have them adopted into different foster families nationwide. This paper describes this transatlantic "struggle of ideas" when it comes to the role of group therapy for traumatized children and the positive impact of such children living collectively in homes. [ABSTRACT FROM AUTHOR]

The physiological or dietary advantages of germinated grains have been the subject of numerous discussions over the past decade. Around 23 million tons of oats are consumed globally, making up a sizeable portion of the global grain market. Oat seedlings contain more protein, beta-glucan, free amino acids, and phenolic compounds than seeds. The progressive neurodegenerative disorder of Alzheimer's is accompanied by worsening memory and cognitive function. A key indicator of this disorder is the unusual buildup of amyloid-beta protein (or Aβ) in human brains. In this context, oat seedling extract (OSE) has been identified as a new therapeutic candidate for AD, due to its antioxidant activity and AD-specific mechanism of action. This study directly investigated how OSE affected AD and its impacts by examining the cognitive function and exploring the inflammatory response mechanism. The dried oat seedlings were grounded finely with a grinder, inserted with 50% fermented ethanol 10 times (w/v), and extracted by stirring for 10 h at 45 °C. After filtering the extract by 0.22 um filter, some of it was used for UHPLC analysis. The results indicated that the treatment with OSE protects against Aβ25–35-induced cytotoxicity in BV2 cells. Tg-5Xfad AD mice had strong deposition of Aβ throughout their brains, while WT mice did not exhibit any such deposition within their brains. A drastic reduction was observed in terms of numbers, as well as the size, of Aβ plaques within Tg-5Xfad AD mice exposed to OSE. This study indicated OSE's neuroprotective impacts against neurodegeneration, synaptic dysfunction, and neuroinflammation induced by amyloid-beta. Our results suggest that OSE acts as a neuroprotective agent to combat AD-specific apoptotic cell death, neuroinflammation, amyloid-beta accumulation, as well as synaptic dysfunction in AD mice's brains. Furthermore, the study indicated that OSE treatment affects JNK/ERK/p38 MAPK signaling, with considerable inhibition in p-JNK, p-p38, and p-ERK levels seen in the brain of OSE-treated Tg-5Xfad AD mice. [ABSTRACT FROM AUTHOR]

Copyright of Polskie Forum Psychologiczne is the property of Kazimierza Wielki University in Bydgoszcz and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Glatiramer acetate (GA) is a well-established treatment option for patients with clinically isolated syndrome and relapsing–remitting multiple sclerosis (MS) with few side effects. The double transgenic mouse model spontaneous opticospinal encephalomyelitis (OSE), based on recombinant myelin oligodendrocyte glycoprotein35-55 reactive T and B cells, mimicks features of chronic inflammation and degeneration in MS and related disorders. Here, we investigated the effects of prophylactic GA treatment on the clinical course, histological alterations and peripheral immune cells in OSE. Objective: To investigate the effects of prophylactic glatiramer acetate (GA) treatment in a mouse model of spontaneous opticospinal encephalomyelitis (OSE). Methods: OSE mice with a postnatal age of 21 to 28 days without signs of encephalomyelitis were treated once daily either with 150 µg GA or vehicle intraperitoneally (i. p.). The animals were scored daily regarding clinical signs and weight. The animals were sacrificed after 30 days of treatment or after having reached a score of 7.0 due to animal care guidelines. We performed immunohistochemistry of spinal cord sections and flow cytometry analysis of immune cells. Results: Preventive treatment with 150 µg GA i. p. once daily significantly reduced clinical disease progression with a mean score of 3.9 ± 1.0 compared to 6.2 ± 0.7 in control animals (p < 0.01) after 30 d in accordance with positive effects on weight (p < 0.001). The immunohistochemistry showed that general inflammation, demyelination or CD11c+ dendritic cell infiltration did not differ. There was, however, a modest reduction of the Iba1+ area (p < 0.05) and F4/80+ area upon GA treatment (p < 0.05). The immune cell composition of secondary lymphoid organs showed a trend towards an upregulation of regulatory T cells, which lacked significance. Conclusions: Preventive treatment with GA reduces disease progression in OSE in line with modest effects on microglia/macrophages. Due to the lack of established prophylactic treatment options for chronic autoimmune diseases with a high risk of disability, our study could provide valuable indications for translational medicine.

Two historical Observing System Experiment (OSE) studies using the ALADIN limited area model and its assimilation system are described. The first study, using an OSE scenario that minimizes the impacts of observations through the lateral boundary conditions, demonstrated the importance of each assimilated terrestrial radiosonde, aircraft, and wind profiler) observations on the analyses and short-range forecasts of the ALADIN/HU model and proved evidence, that the role of conventional observations cannot be even partly taken over by satellite measurements without degradation of the forecast quality. The second study demonstrated that the assimilation of radiosonde observations remains indispensable even with a progressively increasing amount of aircraft measurements. [ABSTRACT FROM AUTHOR]

Adaptive immunity, which plays an important role in the development of atherosclerosis, is mediated by major histocompatibility complex (MHC)-dependent antigen presentation. In atherosclerotic lesions, macrophages constitute an important class of antigen-presenting cells that activate adaptive immune responses to oxidized low-density lipoprotein (OxLDL). It has been reported that autophagy regulates adaptive immune responses by enhancing antigen presentation to MHC class II (MHC-II). In a previous study, we have demonstrated that SYK (spleen tyrosine kinase) regulates generation of reactive oxygen species (ROS) and activation of MAPK8/JNK1 in macrophages. Because ROS and MAPK8 are known to regulate autophagy, in this study we investigated the role of SYK in autophagy, MHC-II expression and adaptive immune response to OxLDL. We demonstrate that OxLDL induces autophagosome formation, MHC-II expression, and phosphorylation of SYK in macrophages. Gene knockout and pharmacological inhibitors of NOX2 and MAPK8 reduced OxLDL-induced autophagy. Using bone marrow-derived macrophages isolated from wild-type and myeloid-specific SYK knockout mice, we demonstrate that SYK regulates OxLDL-induced ROS generation, MAPK8 activation, BECN1-BCL2 dissociation, autophagosome formation and presentation of OxLDL-derived antigens to CD4(+) T cells. ldlr(-/-) syk(-/-) mice fed a high-fat diet produced lower levels of IgG to malondialdehyde (MDA)-LDL, malondialdehyde-acetaldehyde (MAA)-LDL, and OxLDL compared to ldlr(-/-) mice. These results provide new insights into the mechanisms by which SYK regulates MHC-II expression via autophagy in macrophages and may contribute to regulation of adaptive immune responses in atherosclerosis.

This paper describes the process, and lessons learned in a preliminary benefits study of a proposed infrared hyperspectral sounder (HSS) for NOAA’s next generation geostationary satellite program (GeoXO). The valuation of government-owned satellite systems providing a public good with a complex array of instruments is a nascent field of study. Many different sensor configurations are possible, but there are restrictions due to physical and budget constraints. Accounting for economic considerations during the design and planning phase for satellite constellations helps to ensure that the most cost effective instruments are selected. To assess whether the HSS instrument should be included on GeoXO, we applied a value of information approach and found the benefits associated with this instrument are likely to substantially outweigh the costs. Value of information studies often focus on data and information that has a direct use case. Estimating benefits for the HSS is especially challenging because data are not used directly by decision makers. Instead these data along with information from other Earth observing (EO) satellites play a key role in producing the inputs necessary for modern numerical hydrometeorological modeling. We describe strategies to assess the marginal (i.e., incremental) contribution of an instrument that is part of a complex information production process. We make several recommendations that, if implemented, would improve the quality of future studies of this kind. This includes (1) a systems approach to observing system planning, (2) improving the design of observing system experiments (OSSE and OSE), and (3) better tracking of the decisions and needs of end-users, especially those external to the agency.

The physiological or dietary advantages of germinated grains have been the subject of numerous discussions over the past decade. Around 23 million tons of oats are consumed globally, making up a sizeable portion of the global grain market. Oat seedlings contain more protein, beta-glucan, free amino acids, and phenolic compounds than seeds. The progressive neurodegenerative disorder of Alzheimer’s is accompanied by worsening memory and cognitive function. A key indicator of this disorder is the unusual buildup of amyloid-beta protein (or Aβ) in human brains. In this context, oat seedling extract (OSE) has been identified as a new therapeutic candidate for AD, due to its antioxidant activity and AD-specific mechanism of action. This study directly investigated how OSE affected AD and its impacts by examining the cognitive function and exploring the inflammatory response mechanism. The dried oat seedlings were grounded finely with a grinder, inserted with 50% fermented ethanol 10 times (w/v), and extracted by stirring for 10 h at 45 °C. After filtering the extract by 0.22 um filter, some of it was used for UHPLC analysis. The results indicated that the treatment with OSE protects against Aβ25–35-induced cytotoxicity in BV2 cells. Tg-5Xfad AD mice had strong deposition of Aβ throughout their brains, while WT mice did not exhibit any such deposition within their brains. A drastic reduction was observed in terms of numbers, as well as the size, of Aβ plaques within Tg-5Xfad AD mice exposed to OSE. This study indicated OSE’s neuroprotective impacts against neurodegeneration, synaptic dysfunction, and neuroinflammation induced by amyloid-beta. Our results suggest that OSE acts as a neuroprotective agent to combat AD-specific apoptotic cell death, neuroinflammation, amyloid-beta accumulation, as well as synaptic dysfunction in AD mice’s brains. Furthermore, the study indicated that OSE treatment affects JNK/ERK/p38 MAPK signaling, with considerable inhibition in p-JNK, p-p38, and p-ERK levels seen in the brain of OSE-treated Tg-5Xfad AD mice.

Abstract Aircraft play an important role in providing in‐situ upper air observations for use in numerical weather prediction (NWP) models. Aircraft observations of wind, temperature and humidity have been routinely assimilated into NWP models for decades. More recently, the Met Office has installed a network of six receivers to collect Mode‐Select Enhanced Surveillance (Mode‐S EHS) broadcasts and derive wind and temperature information. The paper focuses on the assimilation of high‐frequency (sub‐hourly) Mode‐S winds into the Met Office's hourly cycling high‐resolution NWP model, which employs four‐dimensional‐variational data assimilation (4D‐Var). An observing system experiment (OSE) experiment was carried out to assess the impact of additionally assimilating Mode‐S wind data on the model forecasts in both summer and winter. It is shown that assimilating Mode‐S winds has a positive benefit on the forecast skill in the first 6 hr wind profiles, with an improvement of the mean bias and root mean square (RMS) errors of up to 0.5 m·s−1 in the first hour and 0.3 m·s−1 in the sixth hour. The assimilation of Mode‐S winds is also seen to be beneficial to the near‐surface‐focused UK NWP composite index, and to the hourly precipitation accumulations in fractions skill score (FSS) for forecasts up to 9 hr ahead.

Aircraft play an important role in providing in‐situ upper air observations for use in numerical weather prediction (NWP) models. Aircraft observations of wind, temperature and humidity have been routinely assimilated into NWP models for decades. More recently, the Met Office has installed a network of six receivers to collect Mode‐Select Enhanced Surveillance (Mode‐S EHS) broadcasts and derive wind and temperature information. The paper focuses on the assimilation of high‐frequency (sub‐hourly) Mode‐S winds into the Met Office's hourly cycling high‐resolution NWP model, which employs four‐dimensional‐variational data assimilation (4D‐Var). An observing system experiment (OSE) experiment was carried out to assess the impact of additionally assimilating Mode‐S wind data on the model forecasts in both summer and winter. It is shown that assimilating Mode‐S winds has a positive benefit on the forecast skill in the first 6 hr wind profiles, with an improvement of the mean bias and root mean square (RMS) errors of up to 0.5 m·s−1 in the first hour and 0.3 m·s−1 in the sixth hour. The assimilation of Mode‐S winds is also seen to be beneficial to the near‐surface‐focused UK NWP composite index, and to the hourly precipitation accumulations in fractions skill score (FSS) for forecasts up to 9 hr ahead. [ABSTRACT FROM AUTHOR]

Prunus mume is a traditional ornamental plant, which owed a unique floral scent. However, the diversity of the floral scent in P. mume cultivars with different aroma types was not identified. In this study, the floral scent of eight P. mume cultivars was studied using headspace solid-phase microextraction (HS-SPME) and organic solvent extraction (OSE), combined with gas chromatography-mass spectrometry (GC-MS). In total, 66 headspace volatiles and 74 endogenous extracts were putatively identified, of which phenylpropanoids/benzenoids were the main volatile organic compounds categories. As a result of GC-MS analysis, benzyl acetate (1.55–61.26%), eugenol (0.87–6.03%), benzaldehyde (5.34–46.46%), benzyl alcohol (5.13–57.13%), chavicol (0–5.46%), and cinnamyl alcohol (0–6.49%) were considered to be the main components in most varieties. However, the volatilization rate of these main components was different. Based on the variable importance in projection (VIP) values in the orthogonal partial least-squares discriminate analysis (OPLS-DA), differential components of four aroma types were identified as biomarkers, and 10 volatile and 12 endogenous biomarkers were screened out, respectively. The odor activity value (OAV) revealed that several biomarkers, including (Z)-2-hexen-1-ol, pentyl acetate, (E)-cinnamaldehyde, methyl salicylate, cinnamyl alcohol, and benzoyl cyanide, contributed greatly to the strong-scented, fresh-scented, sweet-scented, and light-scented types of P. mume cultivars. This study provided a theoretical basis for the floral scent evaluation and breeding of P. mume cultivars.

Our understanding of the molecular determinants of cancer is still inadequate because of cancer heterogeneity. Here, using epithelial ovarian cancer (EOC) as a model system, we analyzed a minute amount of patient-derived epithelial cells from either healthy or cancerous tissues by single-shot mass-spectrometry-based phosphoproteomics. Using a multi-disciplinary approach, we demonstrated that primary cells recapitulate tissue complexity and represent a valuable source of differentially expressed proteins and phosphorylation sites that discriminate cancer from healthy cells. Furthermore, we uncovered kinase signatures associated with EOC. In particular, CDK7 targets were characterized in both EOC primary cells and ovarian cancer cell lines. We showed that CDK7 controls cell proliferation and that pharmacological inhibition of CDK7 selectively represses EOC cell proliferation. Our approach defines the molecular landscape of EOC, paving the way for efficient therapeutic approaches for patients. Finally, we highlight the potential of phosphoproteomics to identify clinically relevant and druggable pathways in cancer.

Highlights • Multiple anode configurations allows for the spatial separation of luminescence and UV-fluorescence. • Modulation of the high-voltage during measurements is able to separate the UV-fluorescence from luminescence. • UV-fluorescence may provide a more sensitive measurement of exo-electrons from quartz and feldspars. Abstract The measurement of OSL/TL relies on the initial trapping and subsequent release and recombination of charge in a suitable crystal structure. These measurements allow us to estimate the dose which the crystal has been subjected to and its age. During such processes, however, electrons can be emitted from the sample surface; the ability to measure such exo-electrons can provide information regarding the trapping process, as well as an alternative method of measuring trapped charge. Ideally, TL/OSL can be measured alongside exo-electrons. We will present a new design for an exo-electron detector compatible with the classic Risø reader stimulation head featuring multiple anode configurations and the option for high-voltage modulation to reduce the UV component during TL/OSL measurements. [ABSTRACT FROM AUTHOR]

In this study, we select 18 SG (superconducting gravimeter) records from 15 GGP stations and 99 vertical, 69 horizontal components of IRIS broad-band seismograms during 2004 Sumatra Earthquake to detect the splitting of higher-degree Earth's free oscillations modes (0S4, 0S7~0S10, 2S4, 1S5, 2S5, 1S6) and 12 inner-core sensitive modes (25S2, 27S2, 6S3, 9S3, 13S3, 15S3, 11S4, 18S4, 8S5, 11S5, 23S5, 16S6) by using OSE (optimal sequence estimation) method which only considers self-coupling. Results indicate that OSE can completely isolate singlets of high-degree modes in time-domain, effectively resolve the coupled multiplets independently, and reduce the possibility of mode mixing and end effect, showing that OSE could improve some signals' signal-to-noise ratio. Comparing the results of SG records with seismic data sets suggests that the number of SG records is inadequate to detect all singlets of higher modes. Hence we mainly select plentiful seismograms of IRIS to observe the multiplets of higher modes. We estimate frequencies of the singlets using AR method and evaluate the measurement error using bootstrap method. Besides, we compared the observations with the predictions of PREM-tidal model. This study demonstrates that OSE is effective in isolating singlets of Earth's free oscillations with higher modes. The experimental results may provide constraints to the construction of 3D Earth model. [ABSTRACT FROM AUTHOR]

The splitting and coupling observations for the normal modes may reveal details about large-scale structure of the 3D Earth. In the frequency domain, especially for frequency >1.5 mHz, the mode-coupling should be taken into account as the modes become denser and closer. There are many S-S type clusters below 3 mHz which need to be resolved, such as 0 S 7 - 2 S 3 , 1 S 5 - 2 S 4 , 4 S 1 - 0 S 8 - 3 S 3 , 0 S 9 - 1 S 0 , 1 S 7 - 2 S 6 and other similar clusters. This paper explores whether the obtained splitting singlets of normal modes in high frequency band have higher SNRs and precision under the condition of considering coupling effects into the optimum sequence estimate (OSE). On the basis of the OSE method, we started experiments by extending the equations for vertical and transverse components to the group-coupling scheme that the splitting coefficient matrix B is not only determined by a target multiplet alone but also includes several cross-coupling multiplets. Then the results of self-coupling and group-coupling of the singlets are compared to determine whether the across-multiplet coupling plays an important role in the stripping of the modes. The results showed that the singlets of higher modes could be observed more clearly and resolved more completely by considering group-coupled modes for the narrow-band frequency. After comparing observations with predictions of the PREM−tidal model, the differences may provide valuable information about the deep Earth and further constraints on the 3D structure of the Earth. [ABSTRACT FROM AUTHOR]

Our understanding of the molecular determinants of cancer is still inadequate because of cancer heterogeneity. Here, using epithelial ovarian cancer (EOC) as a model system, we analyzed a minute amount of patient-derived epithelial cells from either healthy or cancerous tissues by single-shot mass-spectrometry-based phosphoproteomics. Using a multi-disciplinary approach, we demonstrated that primary cells recapitulate tissue complexity and represent a valuable source of differentially expressed proteins and phosphorylation sites that discriminate cancer from healthy cells. Furthermore, we uncovered kinase signatures associated with EOC. In particular, CDK7 targets were characterized in both EOC primary cells and ovarian cancer cell lines. We showed that CDK7 controls cell proliferation and that pharmacological inhibition of CDK7 selectively represses EOC cell proliferation. Our approach defines the molecular landscape of EOC, paving the way for efficient therapeutic approaches for patients. Finally, we highlight the potential of phosphoproteomics to identify clinically relevant and druggable pathways in cancer. [ABSTRACT FROM AUTHOR]

The ovarian surface epithelium (OSE) plays an important role in normal ovarian physiology. During each reproductive cycle, the OSE takes part in the cyclical ovulatory ruptures and repair. The aim of this study was to investigate the immunolocalization of nerve growth factor (NGF) and its receptors, tyrosine kinase A (TrkA) and p75, in the OSE cells of the wild ground squirrels during the breeding and nonbreeding seasons. There were marked variations in ovarian weight and size between the breeding and the nonbreeding seasons. Histologically, cuboidal cells and squamous cells were identified in the OSE of both seasons. Yet, stronger immunostaining of NGF, TrkA and p75 were observed in cuboidal cells and squamous cells in the breeding season as compared to the nonbreeding season. In addition, plasma gonadotropin concentrations were higher in the breeding season than in the nonbreeding season, suggesting that the expression patterns of NGF, TrkA and p75 in the OSE were correlated with changes in plasma gonadotropins. These findings suggested that NGF and its receptor TrkA and p75 may be involved in the regulation of seasonal changes in the OSE of wild ground squirrel.in the OSE of wild ground squirrel.

In this study we examined the role of fumaric acid esters (FAE) in a spontaneous and chronic animal model, the opticospinal EAE (OSE). Preventive treatment of dimethylfumarate (DMF) promotes onset of disease in animals treated with high dose DMF. This group also exhibited a significantly exacerbated disease course in a therapeutic treatment as compared to the low dose DMF approach, where less demyelination, macrophage infiltration, and increased Nrf2 expression in the spinal cord were observed. We conclude that low dose DMF treatment is effective in the therapy of the spontaneous opticospinal EAE model and mediates neuroprotective effects via the oxidative stress response pathway. [ABSTRACT FROM AUTHOR]

Reverse time migration has drawn great attention in exploration geophysics because it can be used successfully in areas with large structural and velocity complexity. But its computational cost is considerably high. This paper concerns the fast implementation of the optimized separable approximation of the two-way propagator, which is the most computational expensive step in reverse time migration. On the basis of the low-rank property of the propagator and the idea of randomized algorithm, a randomized method is introduced for optimized separable approximation-based one-step extrapolation. Numerical results of approximating the propagator show that the randomized method is more efficient than the conventional interpolation method. At the same time, numerical experiments of wavefield extrapolation show that the proposed method is much more accurate than the conventional finite-difference plus pseudo-spectrum scheme. Copyright © 2014 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Recent developments in the study of epithelial ovarian cancer have called into question the traditional views regarding the site of tumor initiation. Histopathologic studies and genomic analyses suggest that extra-ovarian sites, like the fallopian tube, may harbor the coveted cell of origin and could therefore contribute significantly to the development of high-grade serous ovarian carcinoma (HG-SOC). Our ability to validate these emerging genomic and pathologic observations and characterize the early transformation events of HG-SOC hinges on the development of novel model systems. Currently, there are only a handful of new model systems that are addressing these concerns. This review will chronicle the convergent evolution of these ovarian cancer model systems in the context of the changing pathologic and genomic understanding of HG-SOC.

We have performed a series of near-real-time observing system experiments with FOAM, the Met Office operational ocean forecasting system. These were conducted in parallel to the operational suite and identical to it except that certain observation types were excluded. At the start of each month the parallel system was reset to the operational restart and a run started with a different observation type excluded: in February -XBT; March -TAO/TRITON; April -Jason-2 altimeter; May -all altimeter; June -AVHRR sea-surface temperature (SST) data; and July -Argo data. We show that the existing ocean observing systems offer a good deal of complementary information. All components of the observing system offer unique, independent information that help initialise, and improve, operational ocean forecasts. Withholding XBTs causes little impact on globally averaged metrics, for example RMS innovations. However, locally we see long-lasting temperature impacts (∼1 °C) near XBT transects. Withholding TAO/TRITON data has a regional impact increasing the tropical Pacific RMS temperature and salinity innovations by 39 and 60%, respectively. Withholding Jason-2 data results in a global 4% increase in the RMS sea-surface height (SSH) innovations, and small-scale changes in temperature and salinity of about 2 °C at 100 m depth, and ∼0.2 psu near the surface. Withholding all altimeter data leads to a 16% increase of the RMS SSH innovations. We also see changes in other model variables similar in magnitude to those from withholding Jason-2, but more widespread. Withholding AVHRR SST data produces significant changes around 1 °C in model temperature at the surface and within the surface mixed layer, but there is little or no effect below this. Withholding Argo data for 1 month leads to a 5% increase in the temperature and salinity innovations, and changes in SSH of up to 5 cm. [ABSTRACT FROM AUTHOR]

Highlights: [•] Observation System Experiments are run with increasing altimeter data streams. [•] Results show increasing mesoscale accuracy as additional altimeter data are added. [•] Frontogenesis is deterministically driven by the mesoscale circulation. [•] Frontogenesis predictability increases as altimeter data streams are added. [•] Frontogenesis is deterministic conditioned on the accurate placement of the mesoscale. [ABSTRACT FROM AUTHOR]

This short paper is concerned with a map of central Japan that the later University of Chicago Professor of Geography, Chauncy Harris, drew in September 1944, when he was assigned as an US Army Private to work at the headquarters of the Research and Analysis Branch, Office of Strategic Services. The map provided a functional classification of Central Japanese cities, highlighting those that specialized in manufacturing. It was precisely these set of cities that were targeted for firebombing by the US Airforce from March 1945. [ABSTRACT FROM AUTHOR]

In this study, the impact of various types of observations on the track forecast of Tropical Cyclone (TC) Jangmi (200815) is examined by using the Weather Research and Forecasting (WRF) model and the corresponding three-dimensional variational (3DVAR) data assimilation system. TC Jangmi is a recurving typhoon that is observed as part of the THORPEX Pacific Asian Regional Campaign (T-PARC). Conventional observations from the Korea Meteorological Administration (KMA) and targeted dropsonde observations from the Dropwindsonde Observations for Typhoon Surveillance near the Taiwan Region (DOTSTAR) were used for a series of observation system experiments (OSEs). We found that the assimilation of observations in oceanic areas is important to analyze environmental flows (such as the North Pacific high) and to predict the recurvature of TC Jangmi. The assimilation of targeted dropsonde observations (DROP) results in a significant impact on the track forecast. Observations of ocean surface winds (QSCAT) and satellite temperature soundings (SATEM) also contribute positively to the track forecast, especially two- to three-day forecasts. The impact of sensitivity guidance such as real-time singular vectors (SVs) was evaluated in additional experiments. [ABSTRACT FROM AUTHOR]

Abstract: Recent experiments have demonstrated that it is possible to measure optically stimulated exoelectron emission (OSE) signals simultaneously with optically stimulated luminescence (OSL) from quartz samples. These experiments provide valuable information on the charge movement in quartz grains. Two specific experiments measured the temperature dependence of the OSL and OSE signals on preheat and stimulation temperature. This paper provides a quantitative description of these experiments by using a previously published theoretical model for photostimulated exoelectron emission (PSEE). The experimental data yield a value of χ∼1.2eV for the work function of quartz. The experimental temperature dependence of the OSE signals is interpreted on the basis of a photo-thermostimulated (PTSEE) process involving the main OSL trap at ∼320°C; this process takes place with a thermal assistance energy estimated at W∼(0.29±0.02)eV. Good quantitative agreement is obtained between theory and experiment by assuming a thermal broadening of the thermal depletion factor for the OSL traps, described by a Gaussian distribution of energies. [Copyright &y& Elsevier]

Abstract: A flow-through Geiger-Müller pancake electron detector attachment has been fitted to a standard Risø TL/OSL reader enabling optically stimulated electrons (OSE) to be measured simultaneously with optically stimulated luminescence (OSL). Using this detector, OSE and OSL measurements from natural quartz samples are studied to examine the possible use of OSE as a chronometer. First the relative variability in OSE and OSL growth curve shapes and the effect of preheat on these are presented, and from these curves, conclusions are drawn concerning the charge movement in natural quartz. Secondly, a dose recovery test shows that OSE can successfully recover a laboratory dose of 300Gy given before any laboratory thermal treatment, for preheating temperatures between 160 and 260°C. Furthermore, for the first time natural OSE decay curves are detected and these signals are used to estimate a burial dose using the single-aliquot regenerative-dose (SAR) procedure. Finally, a comparative study of the equivalent doses estimated using both OSE and OSL from 10 quartz samples are presented, and it is shown that OSE has a significant potential in retrospective dosimetry. [Copyright &y& Elsevier]

Prunus mume is a traditional ornamental plant, which owed a unique floral scent. However, the diversity of the floral scent in P. mume cultivars with different aroma types was not identified. In this study, the floral scent of eight P. mume cultivars was studied using headspace solid-phase microextraction (HS-SPME) and organic solvent extraction (OSE), combined with gas chromatography-mass spectrometry (GC-MS). In total, 66 headspace volatiles and 74 endogenous extracts were putatively identified, of which phenylpropanoids/benzenoids were the main volatile organic compounds categories. As a result of GC-MS analysis, benzyl acetate (1.55–61.26%), eugenol (0.87–6.03%), benzaldehyde (5.34–46.46%), benzyl alcohol (5.13–57.13%), chavicol (0–5.46%), and cinnamyl alcohol (0–6.49%) were considered to be the main components in most varieties. However, the volatilization rate of these main components was different. Based on the variable importance in projection (VIP) values in the orthogonal partial least-squares discriminate analysis (OPLS-DA), differential components of four aroma types were identified as biomarkers, and 10 volatile and 12 endogenous biomarkers were screened out, respectively. The odor activity value (OAV) revealed that several biomarkers, including (Z)-2-hexen-1-ol, pentyl acetate, (E)-cinnamaldehyde, methyl salicylate, cinnamyl alcohol, and benzoyl cyanide, contributed greatly to the strong-scented, fresh-scented, sweet-scented, and light-scented types of P. mume cultivars. This study provided a theoretical basis for the floral scent evaluation and breeding of P. mume cultivars. [ABSTRACT FROM AUTHOR]

Abstract: A flow-through Geiger-Müller pancake electron detector attachment has been fitted to the Risø TL/OSL reader, enabling optically stimulated electrons (OSE) to be measured simultaneously with optically stimulated luminescence (OSL). This detector has been used to provide new insights into charge movement in luminescence phosphors. Here we show that OSE from natural quartz grains gives an easily detectable, reproducible and light sensitive signal, although it is not as intense as OSL. A single sample of natural quartz grains extracted from a sediment is used to investigate the thermal stability and dependence on stimulation temperature of the OSE and OSL signals. The response to dose of these signals is also investigated, and conclusions drawn on the movement of electrons in this natural phosphor. [Copyright &y& Elsevier]

Introduction: The main objectives of the study were to compare the valid index of physical activities according to sex and age, to prove whether the intense level of participation corresponds to the recommendations referring to health of young people, and to identify the group of young people at risk of becoming sedentary. Materials and Methods: The data collection was carried out by means of a questionnaire referred as "Physical sports habits and lifestyles," in the provinces of Almeria, Granada and Murcia, in Spain. The representative samples were composed of OSE 3,249 students and OSPE 2,921 students. Results: The differences of intense participation were significantly favorable to men. The index of physical activity reduced mainly in women. Discussion: Young women are deemed as a pretty sedentary group and at strong risk of becoming sedentary in the future. [ABSTRACT FROM AUTHOR]

To evaluate the effect of HPV16 E6/E7 on drug sensitivity, primary human OSE cells were infected with HPV16 E6/E7 expressing retrovirus and then exposed to chemotherapeutic agents. Apoptosis induced by mitomycin C was dose-dependent in both primary OSE and E6E7/OSE cells. E6E7/OSE cells were more sensitive to mitomycin C than parental OSE cells. HPV16 E6/E7 also sensitized OSE cells to 5-FU and its derivative 5-FUdR, but only at low doses. This phenomenon was also observed in cervical cancer cells and was independent of thymidylate synthase, a target of thymine and thymidine analogues. We conclude that HPV16 E6/E7 specifically modulates the activity of 5-FU and 5-FUdR, and confers OSE cells hypersensitivity to low-dose but not high-dose 5-FU and 5-FUdR. Molecular analysis indicates that induction of p53 and p21, and suppression of pRB are associated with apoptosis induced by 5-FUdR and may partly explain the hypersensitivity of E6E7/OSE cells to low-dose 5-FUdR. [Copyright &y& Elsevier]

Aim. Conflicting evidence exists on whether in vivo morphological characteristics can distinguish Ovarian Surface Epithelium (OSE) of ovaries obtained from women with and without a predisposition to develop female adnexal (ovarian and fallopian tube) carcinoma. This study aims to detect differences in growth potential and morphology that are maintained or specifically expressed in vitro.Study design. Ovarian surfaces were scraped to retrieve OSE cells from 56 women at hereditary high risk for female adnexal carcinoma, of whom 33 are BRCA1 and four are BRCA2 mutation carriers (Predisposed OSE, POSE) and from 26 women without such risk (Non Predisposed OSE, NPOSE). Number of passages and total cell yield until last passage, as well as morphology was compared between both groups. To confirm morphology, the expression of epithelial, mesothelial, and fibroblast markers was assessed.Results. Both POSE and NPOSE cultures displayed similar growth potential and morphology. The expression of epithelial markers cyto-keratins 7 and 8 was similar between both groups. Only in cultures in which cells did not uniformly exhibit these markers, the percentage of cells expressing these markers was significantly lower at last passage when compared to the initial culture. In these latter cultures, cells that were morphologically indistinguishable from fibroblasts were observed. Mesothelial marker calretinin was expressed in 75% of cells of both POSE and NPOSE cultures and correlates with cyto-keratins 7 and 8 expression. CA 125 expression was equally low in POSE and NPOSE cultures (4.3%). Fibroblast markers FSM and vimentin were expressed in 100% and collagen IV was expressed in 16% of cells in all cultures.Conclusion. OSE cells derived from women with a hereditary predisposition to develop female adnexal cancer possess similar in vitro characteristics as OSE from women without this predisposition. On basis of our results, it seems advisable to study only 100% cyto-keratins 7 and 8 positive OSE cultures, since contamination of fibroblasts in some primary OSE cultures cannot be ruled out. [Copyright &y& Elsevier]

Estrogen promotes the growth of some ovarian cancer cells at nanomolar concentrations, but has been shown to inhibit growth of normal ovarian surface epithelial (OSE) cells at micromolar concentrations (1 μg/ml). OSE cells express the estrogen receptor (ER)-α, and are the source of 90% of ovarian cancers. The potential sensitivity of OSE cells to estrogen stresses the importance of understanding the estrogen-dependent mechanisms at play in OSE proliferation and transformation, as well as in anticancer treatment. We investigated the effects of estradiol on cell proliferation in vitro, and demonstrate an intracellular locus of action of estradiol in cultured rhesus ovarian surface epithelial (RhOSE) cells. We show that ovarian and breast cells are growth-inhibited by micromolar concentrations of estradiol, and that this inhibition correlates with estrogen receptor expression. We further show that normal rhesus OSE cells do not activate ERK or Akt in response to estradiol, nor does estradiol block the ability of serum to stimulate ERK or induce cyclin D expression. Contrarily, estradiol inhibits serum-dependent retinoblastoma protein (Rb) phosphorylation and blocks DNA synthesis. This inhibition does not formally arrest cells, and is reversible within hours of estrogen withdrawal. Our data are consistent with growth inhibition by activation of Rb and indicate that sensitivity to hormone therapy in anticancer treatment can be modulated by cell cycle regulators downstream of the estrogen receptor. [Copyright &y& Elsevier]

In this paper we explore some recent trends in the financial market and also report some studies of the Singapore futures markets. A characterization of trends shows that national securities markets are much closer than before. This means the linkages between securities and their derivatives and amongst themselves have be come much stronger. Secondly, the advent of sophisticated risk products and instruments and the knowledge to use them effectively would become a common theme together with the idea of value enhancements. Thirdly, computerizations and the internet will play an increasingly important role. So will empirical financial research become increasingly microscopic. The discussion will be supported by the experiences of the Singapore futures markets and various empirical research evidences. The paper also provides a detailed study of causality-in-variance test of information transmission between SIMEX and Osaka Stock Exchange on the Nikkei 225 stock index futures trading prior to, during, and immediately after the announcement of the collapse of Barings. The results are indicative of very strong international market linkages and a portent of things to come. [ABSTRACT FROM AUTHOR]

Ovarian surface epithelium (OSE) is a cell monolayer surrounding the ovary; it is involved in the regulation of the ovulatory process and the genesis of ovarian carcinoma. However, intercellular messengers regulating signaling events, like proliferation in the OSE, have not been completely described. Purines have emerged as novel intercellular messengers in the ovary, in which expression of purinergic receptors has been reported in different cell types. In the present work, we described the functional expression of P2Y2 receptor (P2Y2R), a purinergic receptor widely associated with cell proliferation, in the OSE. The expression of P2Y2R by immunofluorescence and RT-PCR, and its functionality by Ca 2+ recording was demonstrated in primary cultured OSE. Functional expression of P2Y2R was also exhibited in situ, by recording of intracellular Ca 2+ release and detection of ERK phosphorylation after injection of a selective agonist into the ovarian bursa. Furthermore, P2Y2R activation with UTPγS, in situ, induced cell proliferation at 24 h, whereas continuous stimulation of P2Y2R during a complete estrous cycle significantly modified the size distribution of the follicular population. This is the first evidence of the functional expression of purinergic P2Y2R in the OSE and opens new perspectives on the roles played by purines in ovarian physiology., (© 2021 Wiley Periodicals LLC.)