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27 results on '"Obata, Yuji"'

1. Edge-Colorings of Weighted Graphs : (Extended Abstract)

Author

Obata, Yuji, Nishizeki, Takao, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Kobsa, Alfred, Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Weikum, Gerhard, Series editor, Rahman, M. Sohel, editor, and Tomita, Etsuji, editor

Published
2015
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2. Approximation Algorithms for Bandwidth Consecutive Multicolorings : (Extended Abstract)

Author

Obata, Yuji, Nishizeki, Takao, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Kobsa, Alfred, editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Pandu Rangan, C., editor, Steffen, Bernhard, editor, Terzopoulos, Demetri, editor, Tygar, Doug, editor, Weikum, Gerhard, editor, Chen, Jianer, editor, Hopcroft, John E., editor, and Wang, Jianxin, editor

Published
2014
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9. Mechanistic Insights into the Activation of Soluble Guanylate Cyclase by Carbon Monoxide: A Multistep Mechanism Proposed for the BAY 41-2272 Induced Formation of 5-Coordinate CO–Heme

Author

Makino, Ryu, Obata, Yuji, Tsubaki, Motonari, Iizuka, Tetsutaro, Hamajima, Yuki, Kato-Yamada, Yasuyuki, Mashima, Keisuke, and Shiro, Yoshitsugu

Abstract

Soluble guanylate cyclase (sGC) is a heme-containing enzyme that catalyzes cGMP production upon sensing NO. While the CO adduct, sGC–CO, is much less active, the allosteric regulator BAY 41-2272 stimulates the cGMP productivity to the same extent as that of sGC–NO. The stimulatory effect has been thought to be likely associated with Fe-His bond cleavage leading to 5-coordinate CO–heme, but the detailed mechanism remains unresolved. In this study, we examined the mechanism under the condition including BAY 41-2272, 2′-deoxy-3′-GMP and foscarnet. The addition of these effectors caused the original 6-coordinate CO–heme to convert to an end product that was an equimolar mixture of a 5- and a new 6-coordinate CO–heme, as assessed by IR spectral measurements. The two types of CO–hemes in the end product were further confirmed by CO dissociation kinetics. Stopped-flow measurements under the condition indicated that the ferrous sGC bound CO as two reversible steps, where the primary step was assigned to the full conversion of the ferrous enzyme to the 6-coordinate CO–heme, and subsequently followed by the slower second step leading a partial conversion of the 6-coordinate CO–heme to the 5-coordinate CO–heme. The observed rates for both steps linearly depended on CO concentrations. The unexpected CO dependence of the rates in the second step supports a multistep mechanism, in which the 5-coordinate CO–heme is led by CO release from a putative bis-carbonyl intermediate that is likely provided by the binding of a second CO to the 6-coordinate CO–heme. This mechanism provides a new aspect on the activation of sGC by CO.

Published
2024
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27. Expression of N19S-SOD1, an SOD1 mutant found in sporadic amyotrophic lateral sclerosis patients, induces low-grade motoneuronal toxicity.

Author

Obata Y, Niikura T, Kanekura K, Hashimoto Y, Kawasumi M, Kita Y, Aiso S, Matsuoka M, and Nishimoto I

Subjects
Amyotrophic Lateral Sclerosis pathology, Animals, CHO Cells, Cell Death physiology, Cricetinae, Humans, Immunoblotting, Mice, Mutation, Oxidative Stress, Superoxide Dismutase genetics, Superoxide Dismutase-1, Transfection, Amyotrophic Lateral Sclerosis genetics, Motor Neurons pathology, Superoxide Dismutase metabolism
Abstract

Amyotrophic lateral sclerosis (ALS) is the most common fatal motor neuron disease. It has been generally accepted that the proapoptotic property of the familial ALS (FALS)-linked mutant SOD1 genes plays an important role in the pathogenesis of some FALS cases. We found here that expression of N19S-SOD1, a novel SOD1 mutant originally found in a sporadic ALS patient, induces lower grade death in NSC34 cells than FALS-linked mutant SOD1. In agreement, intracytoplasmic aggregate formation and SOD1 polymerization are less prominently induced by ectopic expression of N19S-SOD1 than FALS-linked mutant SOD1. We further found that additional cell stresses, such as inhibition of proteasomal activity or up-regulation of intracellular oxidative stress, enhance N19S-SOD1-induced aggregate formation and polymerization of N19S-SOD1. Such analysis of the intracellular polymerization and the ubiquitination of N19S-SOD1 have further suggested that it is recognized as a misfolded protein, like FALS-linked mutant SOD1, whereas wild-type SOD1 is not. Altogether, it is speculated that the N19S mutation of SOD1 in cooperation with associated cell stresses contributes to the onset of ALS as a risk factor.

Published
2005
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