Search

Your search keyword '"Oberholzer, Michael"' showing total 49 results
Start Over Author "Oberholzer, Michael"
49 results on '"Oberholzer, Michael"'

1. Frequency and evolution of sleep-wake disturbances after ischemic stroke: A 2-year prospective study of 437 patients

Author

Duss, Simone B., Bauer-Gambelli, Stefan A., Bernasconi, Corrado, Dekkers, Martijn P.J., Gorban-Peric, Corina, Kuen, Doris, Seiler, Andrea, Oberholzer, Michael, Alexiev, Filip, Lippert, Julian, Brill, Anne-Kathrin, Ott, Sebastian R., Zubler, Frédéric, Horvath, Thomas, Schmidt, Markus H., Manconi, Mauro, and Bassetti, Claudio L.A.

Published
2023
Full Text
View/download PDF

2. Insect Stage-Specific Receptor Adenylate Cyclases Are Localized to Distinct Subdomains of the Trypanosoma brucei Flagellar Membrane

Author

Saada, Edwin A, Kabututu, Z Pius, Lopez, Miguel, Shimogawa, Michelle M, Langousis, Gerasimos, Oberholzer, Michael, Riestra, Angelica, Jonsson, Zophonias O, Wohlschlegel, James A, and Hill, Kent L

Subjects
Biochemistry and Cell Biology, Biological Sciences, Vector-Borne Diseases, Infectious Diseases, 2.2 Factors relating to the physical environment, Infection, Adenylyl Cyclases, Animals, Cell Line, Flagella, Insecta, Life Cycle Stages, Protein Transport, Protozoan Proteins, RNA, Messenger, Trypanosoma brucei brucei, Immunology, Microbiology
Abstract

Increasing evidence indicates that the Trypanosoma brucei flagellum (synonymous with cilium) plays important roles in host-parasite interactions. Several studies have identified virulence factors and signaling proteins in the flagellar membrane of bloodstream-stage T. brucei, but less is known about flagellar membrane proteins in procyclic, insect-stage parasites. Here we report on the identification of several receptor-type flagellar adenylate cyclases (ACs) that are specifically upregulated in procyclic T. brucei parasites. Identification of insect stage-specific ACs is novel, as previously studied ACs were constitutively expressed or confined to bloodstream-stage parasites. We show that procyclic stage-specific ACs are glycosylated, surface-exposed proteins that dimerize and possess catalytic activity. We used gene-specific tags to examine the distribution of individual AC isoforms. All ACs examined localized to the flagellum. Notably, however, while some ACs were distributed along the length of the flagellum, others specifically localized to the flagellum tip. These are the first transmembrane domain proteins to be localized specifically at the flagellum tip in T. brucei, emphasizing that the flagellum membrane is organized into specific subdomains. Deletion analysis reveals that C-terminal sequences are critical for targeting ACs to the flagellum, and sequence comparisons suggest that differential subflagellar localization might be specified by isoform-specific C termini. Our combined results suggest insect stage-specific roles for a subset of flagellar adenylate cyclases and support a microdomain model for flagellar cyclic AMP (cAMP) signaling in T. brucei. In this model, cAMP production is compartmentalized through differential localization of individual ACs, thereby allowing diverse cellular responses to be controlled by a common signaling molecule.

Published
2014

3. Adressen

Author

Bassetti, Claudio, primary, Bopp-Kistler, Irene, additional, Bosshard, Georg, additional, Cassai, Lilian de, additional, Diener, Suzie, additional, Felbecker, Ansgar, additional, Früh, Severin, additional, Göhl, Kristina, additional, Hägele-Link, Stefan, additional, Herwig, Uwe, additional, Kägi, Georg, additional, Limmroth, Volker, additional, Meyer-Heim, Tatjana, additional, Müri, René, additional, Oberholzer, Michael, additional, Seeger, Rolf, additional, Tettenborn, Barbara, additional, Unschuld, Paul G., additional, Welz, Dieter, additional, and Zieglgänsberger, Dominik, additional

Published
2019
Full Text
View/download PDF

4. Demenzformen

Author

Oberholzer, Michael, primary, Bassetti, Claudio L., additional, Müri, René M., additional, Göhl, Kristina, additional, Felbecker, Ansgar, additional, Kägi, Georg, additional, de Cassai, Lilian, additional, Herwig, Uwe, additional, and Zieglgänsberger, Dominik, additional

Published
2019
Full Text
View/download PDF

5. Anti‐kelchlike protein 11 antibody‐associated encephalitis: Two case reports and review of the literature

Author

León Betancourt, Alejandro, primary, Schwarzwald, Anina, additional, Millonig, Alban, additional, Oberholzer, Michael, additional, Sabater, Lidia, additional, Hammer, Helly, additional, Kamber, Nicole, additional, Diem, Lara, additional, Chan, Andrew, additional, Hoepner, Robert, additional, Salmen, Anke, additional, and Friedli, Christoph, additional

Published
2023
Full Text
View/download PDF

6. Anti-Kelch-like protein 11 (KLHL11) antibody associated encephalitis -Two case reports and review of the literature

Author

Léon Betancourt, Alejandro, Schwarzwald, Anina, Millonig, Alban, Oberholzer, Michael, Sabater, Lidia, Hammer, Helly, Kamber, Nicole, Diem, Lara, Chan, Andrew, Hoepner, Robert, Salmen, Anke, and Friedli, Christoph

Subjects
610 Medizin und Gesundheit
Abstract

INTRODUCTION Kelch-like protein 11 antibodies (KLHL11-IgG) were first described in 2019 as a marker of paraneoplastic neurological syndromes (PNS). They have mostly been associated with testicular germ cell tumours (tGCT). METHODS Report of two patients with KLHL11-IgG encephalitis and comprehensive review of literature. RESULTS Patient 1 had been in remission from a tGCT 10 years prior. He developed episodic vertigo and diplopia progressing over a few days. Treatment with corticosteroids (CS) was started few days after symptom-onset. Patient 2 had transient diplopia which resolved spontaneously. Visual problems persisted for 7 months, when he additionally developed a progressive cerebellar syndrome. 1 year after onset, CS treatment was started. Initial MRIs were unremarkable in both patients, but analysis of cerebrospinal fluid (CSF) revealed chronic inflammation. KLHL11-IgG was positive in both patients (Patient 1 only in CSF, patient 2 in serum). Neoplastic screening has so far not revealed any signs of active underlying malignancy. We found 15 publications of 112 patients in total with KLHL11-IgG encephalitis. Most patients (n=82) had a cerebellar syndrome with or without signs of rhombencephalitis. The most common symptoms were ataxia (n=82) and vertigo (n=47), followed by oculomotor disturbances (n=35) and hearing disorders (n=31). 80 of 84 patients had a GCT as an underlying tumour. CONCLUSION Our cases demonstrate classical symptoms of KLHL11-IgG encephalitis. An early diagnosis and therapy is imperative. As with other PNS, clinical awareness is needed and further studies are required especially in regard to therapeutic management.

Published
2023
Full Text
View/download PDF

7. Cerebrospinal fluid HIV-1 escape in patients with neurocognitive symptoms: pooled data from a neuro-HIV platform and the NAMACO study

Author

Filippidis, Paraskevas, Damas, Jose, Viala, Benjamin, Assal, Frederic, Tshikung, Olivier Nawej, Tarr, Philip, Derfuss, Tobias, Oberholzer, Michael, Jelcic, Ilijas, Hundsberger, Thomas, Sacco, Leonardo, Cavassini, Matthias, Du Pasquier, Renaud, and Darling, Katharine E A

Subjects
610 Medicine & health
Abstract

BACKGROUND Despite modern antiretroviral therapy, HIV-1 RNA escape into the cerebrospinal fluid (CSF) may occur. We examined the prevalence of and factors associated with CSF HIV-1 escape among people living with HIV (PLWH) in Switzerland. SETTING The Neurocognitive Assessment in the Metabolic and Aging Cohort (NAMACO) study is an ongoing, prospective, longitudinal, multicenter study within the Swiss HIV Cohort Study. The neuro-HIV platform is a multi-disciplinary, single-day outpatient consultation at Lausanne University Hospital. METHODS We pooled data from the NAMACO study and the neuro-HIV platform participants who underwent lumbar puncture (LP) between 2011 and 2019. Both patient groups had neurocognitive symptoms. CSF HIV-1 escape was defined as the presence of quantifiable CSF HIV-1 RNA when plasma HIV-1 RNA was suppressed or CSF HIV-1 RNA greater than plasma HIV-1 RNA when the latter was detectable. RESULTS Of 1166 PLWH assessed, 288 underwent LP. CSF HIV-1 escape was observed in 25 PLWH (8.7%) of whom 19 (76%) had supressed plasma HIV-1 RNA. Characteristics of PLWH were comparable whether they had CSF HIV-1 escape or not, including comorbidities, time since HIV diagnosis (15 vs 16 years, p=0.9), median CD4 nadir (158.5/mm3 vs 171/mm3, p=0.6), antiretroviral CSF-Penetration-Effectiveness score (7 vs 7 points, p=0.8), neurocognitive diagnosis based on Frascati criteria and radiological findings. CONCLUSIONS In this large pooled sample of PLWH with neurocognitive symptoms, CSF HIV-1 escape occurred in 8.7% of PLWH. PLWH with CSF HIV-1 escape presented no distinctive clinical or paraclinical characteristics. We conclude that LP is unavoidable in confirming CSF HIV-1 escape.

Published
2023
Full Text
View/download PDF

12. Stroke and splenic infarct in a 17-year-old patient with COVID-associated hypercoagulable state and relative ADAMTS13 deficiency

Author

Pistor, Maximilian, Hoffmann, Angelika, Horvath, Thomas, Oberholzer, Michael, Arnold, Marcel, and Pilgram-Pastor, Sara Magdalena

Subjects
Stroke, endovascular therapy, SARS-CoV2, hypercoagulable state, young stroke, COVID-19, 610 Medicine & health, Neurosciences. Biological psychiatry. Neuropsychiatry, cardiovascular diseases, ADAMTS13, COVID, RC321-571
Abstract

In this case report, we present the case of a 17-year-old stroke patient suffering from coronavirus disease (COVID)-19. He was successfully treated with intravenous and endovascular treatment. After extensive work-up, a hypercoagulable state due to the COVID-19 infection was assumed as probable cause of stroke.

Published
2021
Full Text
View/download PDF

20. Clinical Utility of Advanced Microbiology Testing Tools

Author

ASM Corporate Council, Osiecki, John, Dunn, James, ASM Clinical and Public Health Microbiology Committee, Campos, Joe, Oberholzer, Michael, Whitfield, Natalie, Atrzadeh, Faranak, Burnham, Carey-Ann D., Pedersen, David, Meduri, John, Mathews, Charles, McNult, Peggy, Jones, Stephen, Cavalieri, Stephen, Miller, Melissa B., Newton, Duane, Newhouse, Chris, and Sweeney, Nicole

Abstract

Advanced microbiology technologies are rapidly changing our ability to diagnose infections, improve patient care, and enhance clinical workflow. These tools are increasing the breadth, depth, and speed of diagnostic data generated per patient, and testing is being moved closer to the patient through rapid diagnostic technologies, including point-of-care (POC) technologies. While select stakeholders have an appreciation of the value/importance of improvements in the microbial diagnostic field, there remains a disconnect between clinicians and some payers and hospital administrators in terms of understanding the potential clinical utility of these novel technologies. Therefore, a key challenge for the clinical microbiology community is to clearly articulate the value proposition of these technologies to encourage payers to cover and hospitals to adopt advanced microbiology tests. Specific guidance on how to define and demonstrate clinical utility would be valuable. Addressing this challenge will require alignment on this topic, not just by microbiologists but also by primary care and emergency room (ER) physicians, infectious disease specialists, pharmacists, hospital administrators, and government entities with an interest in public health. In this article, we discuss how to best conduct clinical studies to demonstrate and communicate clinical utility to payers and to set reasonable expectations for what diagnostic manufacturers should be required to demonstrate to support reimbursement from commercial payers and utilization by hospital systems.

Published
2019
Full Text
View/download PDF

22. Alkynamide phthalazinones as a new class of TbrPDEB1 inhibitors (Part 2)

Author

de Heuvel, Erik, primary, Singh, Abhimanyu K., additional, Boronat, Pierre, additional, Kooistra, Albert J., additional, van der Meer, Tiffany, additional, Sadek, Payman, additional, Blaazer, Antoni R., additional, Shaner, Nathan C., additional, Bindels, Daphne S., additional, Caljon, Guy, additional, Maes, Louis, additional, Sterk, Geert Jan, additional, Siderius, Marco, additional, Oberholzer, Michael, additional, de Esch, Iwan J.P., additional, Brown, David G., additional, and Leurs, Rob, additional

Published
2019
Full Text
View/download PDF

23. Clinical Utility of Advanced Microbiology Testing Tools

Author

Miller, Melissa B., primary, Atrzadeh, Faranak, additional, Burnham, Carey-Ann D., additional, Cavalieri, Stephen, additional, Dunn, James, additional, Jones, Stephen, additional, Mathews, Charles, additional, McNult, Peggy, additional, Meduri, John, additional, Newhouse, Chris, additional, Newton, Duane, additional, Oberholzer, Michael, additional, Osiecki, John, additional, Pedersen, David, additional, Sweeney, Nicole, additional, Whitfield, Natalie, additional, and Campos, Joe, additional

Published
2019
Full Text
View/download PDF

25. Sleepwalking in Parkinson's disease: a questionnaire-based survey

Author

Oberholzer, Michael, Poryazova, Rositsa, Bassetti, Claudio, Oberholzer, Michael, Poryazova, Rositsa, and Bassetti, Claudio

Abstract

Sleepwalking (SW) corresponds to a complex sleep-associated behavior that includes locomotion, mental confusion, and amnesia. SW is present in about 10% of children and 2-3% of adults. In a retrospective series of 165 patients with Parkinson's disease (PD), we found adult-onset ("de novo”) SW "de novo” in six (4%) of them. The aim of this study was to assess prospectively and systematically the frequency and characteristics of SW in PD patients. A questionnaire including items on sleep quality, sleep disorders, and specifically also SW and REM sleep behavior disorder (RBD), PD characteristics and severity, was sent to the members of the national PD patients organization in Switzerland. In the study, 36/417 patients (9%) reported SW, of which 22 (5%) had adult-onset SW. Patients with SW had significantly longer disease duration (p=0.035), they reported more often hallucinations (p=0.004) and nightmares (p=0.003), and they had higher scores, suggestive for RBD in a validated questionnaire (p=0.001). Patients with SW were also sleepier (trend to a higher Epworth Sleepiness Scale score, p=0.055). Our data suggest that SW in PD patients is (1) more common than in the general population, and (2) is associated with RBD, nightmares, and hallucinations. Further studies including polysomnographic recordings are needed to confirm the results of this questionnaire-based analysis, to understand the relationship between SW and other nighttime wandering behaviors in PD, and to clarify the underlying mechanisms

Published
2018

32. Insect Stage-Specific Receptor Adenylate Cyclases Are Localized to Distinct Subdomains of the Trypanosoma brucei Flagellar Membrane

Author

Saada, Edwin A., primary, Kabututu, Z. Pius, additional, Lopez, Miguel, additional, Shimogawa, Michelle M., additional, Langousis, Gerasimos, additional, Oberholzer, Michael, additional, Riestra, Angelica, additional, Jonsson, Zophonias O., additional, Wohlschlegel, James A., additional, and Hill, Kent L., additional

Published
2014
Full Text
View/download PDF

35. Social parasites

Author

Lopez, Miguel A, primary, Nguyen, HoangKim T, additional, Oberholzer, Michael, additional, and Hill, Kent L, additional

Published
2011
Full Text
View/download PDF

36. Independent Analysis of the Flagellum Surface and Matrix Proteomes Provides Insight into Flagellum Signaling in Mammalian-infectious Trypanosoma brucei

Author

Oberholzer, Michael, primary, Langousis, Gerasimos, additional, Nguyen, HoangKim T., additional, Saada, Edwin A., additional, Shimogawa, Michelle M., additional, Jonsson, Zophonias O., additional, Nguyen, Steven M., additional, Wohlschlegel, James A., additional, and Hill, Kent L., additional

Published
2011
Full Text
View/download PDF

43. Cyclic nucleotide specific phosphodiesterases of the kinetoplastida: A unified nomenclature

Author

Kunz, Stefan, primary, Beavo, Joseph A., additional, D’Angelo, Maximiliano A., additional, Flawia, Mirtha M., additional, Francis, Sharron H., additional, Johner, Andrea, additional, Laxman, Sunil, additional, Oberholzer, Michael, additional, Rascon, Ana, additional, Shakur, Yasmin, additional, Wentzinger, Laurent, additional, Zoraghi, Roya, additional, and Seebeck, Thomas, additional

Published
2006
Full Text
View/download PDF

45. The Trypanosoma brucei cAMP phosphodiesterases TbrPDEB1 and TbrPDEB2: flagellar enzymes that are essential for parasite virulence.

Author

Oberholzer, Michael, Marti, Gabriela, Baresic, Mario, Kunz, Stefan, Hemphill, Andrew, and Seebeck, Thomas

Subjects
*NUCLEOTIDES, *PHOSPHODIESTERASES, *CELLULAR signal transduction, *TARGETED drug delivery, *GENOMICS, *FLAGELLA (Microbiology), *CYTOPLASM
Abstract

Cyclic nucleotide specific phosphodiesterases (PDEs) are pivotal regulators of cellular signaling. They are also important drug targets. Besides catalytic activity and substrate specificity, their subcellular localization and interaction with other cell components are also functionally important. In contrast to the mammalian PDEs, the significance of PDEs in protozoal pathogens remains mostly unknown. The genome of Trypanosoma brucei, the causative agent of human sleeping sickness, codes for five different PDEs. Two of these, TbrPDEB1 and TbrPDEB2, are closely similar, cAMP-specific PDEs containing two GAF-domains in their N-terminal regions. Despite their similarity, these two PDEs exhibit different subcellular localizations. TbrPDEB1 is located in the flagellum, whereas TbrPDEB2 is distributed between flagellum and cytoplasm. RNAi against the two mRNAs revealed that the two enzymes can complement each other but that a simultaneous ablation of both leads to cell death in bloodstream form trypanosomes. RNAi against TbrPDEB1 and TbrPDEB2 also functions in vivo where it completely prevents infection and eliminates ongoing infections. Our data demonstrate that TbrPDEB1 and TbrPDEB2 are essential for virulence, making them valuable potential targets for new PDE-inhibitor based trypanocidal drugs. Furthermore, they are compatible with the notion that the flagellum of T. brucei is an important site of cAMP signaling. [ABSTRACT FROM AUTHOR]

Published
2007
Full Text
View/download PDF

46. Insect Stage-Specific Receptor Adenylate Cyclases Are Localized to Distinct Subdomains of the Trypanosoma bruceiFlagellar Membrane

Author

Saada, Edwin A., Kabututu, Z. Pius, Lopez, Miguel, Shimogawa, Michelle M., Langousis, Gerasimos, Oberholzer, Michael, Riestra, Angelica, Jonsson, Zophonias O., Wohlschlegel, James A., and Hill, Kent L.

Abstract

ABSTRACTIncreasing evidence indicates that the Trypanosoma bruceiflagellum (synonymous with cilium) plays important roles in host-parasite interactions. Several studies have identified virulence factors and signaling proteins in the flagellar membrane of bloodstream-stage T. brucei, but less is known about flagellar membrane proteins in procyclic, insect-stage parasites. Here we report on the identification of several receptor-type flagellar adenylate cyclases (ACs) that are specifically upregulated in procyclic T. bruceiparasites. Identification of insect stage-specific ACs is novel, as previously studied ACs were constitutively expressed or confined to bloodstream-stage parasites. We show that procyclic stage-specific ACs are glycosylated, surface-exposed proteins that dimerize and possess catalytic activity. We used gene-specific tags to examine the distribution of individual AC isoforms. All ACs examined localized to the flagellum. Notably, however, while some ACs were distributed along the length of the flagellum, others specifically localized to the flagellum tip. These are the first transmembrane domain proteins to be localized specifically at the flagellum tip in T. brucei, emphasizing that the flagellum membrane is organized into specific subdomains. Deletion analysis reveals that C-terminal sequences are critical for targeting ACs to the flagellum, and sequence comparisons suggest that differential subflagellar localization might be specified by isoform-specific C termini. Our combined results suggest insect stage-specific roles for a subset of flagellar adenylate cyclases and support a microdomain model for flagellar cyclic AMP (cAMP) signaling in T. brucei. In this model, cAMP production is compartmentalized through differential localization of individual ACs, thereby allowing diverse cellular responses to be controlled by a common signaling molecule.

Published
2014
Full Text
View/download PDF

47. Dictyostelium Dock180-related RacGEFs Regulate the Actin Cytoskeleton during Cell Motility

Author

Para, Alessia, Krischke, Miriam, Merlot, Sylvain, Shen, Zhouxin, Oberholzer, Michael, Lee, Susan, Briggs, Steven, and Firtel, Richard A.

Abstract

Cell motility of amoeboid cells is mediated by localized F-actin polymerization that drives the extension of membrane protrusions to promote forward movements. We show that deletion of either of two members of the Dictyostelium Dock180 family of RacGEFs, DockA and DockD, causes decreased speed of chemotaxing cells. The phenotype is enhanced in the double mutant and expression of DockA or DockD complements the reduced speed of randomly moving DockD null cells' phenotype, suggesting that DockA and DockD are likely to act redundantly and to have similar functions in regulating cell movement. In this regard, we find that overexpressing DockD causes increased cell speed by enhancing F-actin polymerization at the sites of pseudopod extension. DockD localizes to the cell cortex upon chemoattractant stimulation and at the leading edge of migrating cells and this localization is dependent on PI3K activity, suggesting that DockD might be part of the pathway that links PtdIns(3,4,5)P3production to F-actin polymerization. Using a proteomic approach, we found that DdELMO1 is associated with DockD and that Rac1A and RacC are possible in vivo DockD substrates. In conclusion, our work provides a further understanding of how cell motility is controlled and provides evidence that the molecular mechanism underlying Dock180-related protein function is evolutionarily conserved.

Published
2009

48. Sleepwalking in Parkinson's disease: a questionnaire-based survey

Author

Oberholzer, Michael, Poryazova, Rositsa, Bassetti, Claudio, Oberholzer, Michael, Poryazova, Rositsa, and Bassetti, Claudio

Abstract

Sleepwalking (SW) corresponds to a complex sleep-associated behavior that includes locomotion, mental confusion, and amnesia. SW is present in about 10% of children and 2-3% of adults. In a retrospective series of 165 patients with Parkinson's disease (PD), we found adult-onset ("de novo”) SW "de novo” in six (4%) of them. The aim of this study was to assess prospectively and systematically the frequency and characteristics of SW in PD patients. A questionnaire including items on sleep quality, sleep disorders, and specifically also SW and REM sleep behavior disorder (RBD), PD characteristics and severity, was sent to the members of the national PD patients organization in Switzerland. In the study, 36/417 patients (9%) reported SW, of which 22 (5%) had adult-onset SW. Patients with SW had significantly longer disease duration (p=0.035), they reported more often hallucinations (p=0.004) and nightmares (p=0.003), and they had higher scores, suggestive for RBD in a validated questionnaire (p=0.001). Patients with SW were also sleepier (trend to a higher Epworth Sleepiness Scale score, p=0.055). Our data suggest that SW in PD patients is (1) more common than in the general population, and (2) is associated with RBD, nightmares, and hallucinations. Further studies including polysomnographic recordings are needed to confirm the results of this questionnaire-based analysis, to understand the relationship between SW and other nighttime wandering behaviors in PD, and to clarify the underlying mechanisms

49. Cerebrospinal Fluid HIV-1 Escape in Patients With Neurocognitive Symptoms: Pooled Data From a Neuro-HIV Platform and the NAMACO Study.

Author

Filippidis P, Damas J, Viala B, Assal F, Nawej Tshikung O, Tarr P, Derfuss T, Oberholzer M, Jelcic I, Hundsberger T, Sacco L, Cavassini M, Du Pasquier R, and Darling KEA

Subjects
Humans, Cohort Studies, Prospective Studies, RNA, Viral, Cerebrospinal Fluid, Viral Load, HIV-1 genetics, HIV Infections drug therapy
Abstract

Background: Despite modern antiretroviral therapy, human immunodeficiency virus-1 (HIV-1) ribonucleic acid (RNA) escape into the cerebrospinal fluid (CSF) may occur. We examined the prevalence of and factors associated with CSF HIV-1 escape among people living with HIV (PLWH) in Switzerland., Setting: The Neurocognitive Assessment in the Metabolic and Aging Cohort study is an ongoing, prospective, longitudinal, multicenter study within the Swiss HIV Cohort Study. The neuro-HIV platform is a multidisciplinary, single-day outpatient consultation at Lausanne University Hospital., Methods: We pooled data from the Neurocognitive Assessment in the Metabolic and Aging Cohort study and the neuro-HIV platform participants who underwent lumbar puncture between 2011 and 2019. Both patient groups had neurocognitive symptoms. Cerebrospinal fluid HIV-1 escape was defined as the presence of quantifiable CSF HIV-1 RNA when plasma HIV-1 RNA was suppressed or CSF HIV-1 RNA greater than plasma HIV-1 RNA when the latter was detectable., Results: Of 1166 PLWH assessed, 288 underwent lumbar puncture. Cerebrospinal fluid HIV-1 escape was observed in 25 PLWH (8.7%) of whom 19 (76%) had suppressed plasma HIV-1 RNA. Characteristics of PLWH were comparable whether they had CSF HIV-1 escape or not, including comorbidities, time since HIV diagnosis (15 vs 16 years, P = 0.9), median CD4 nadir (158.5/mm 3 vs 171/mm 3 , P = 0.6), antiretroviral CSF penetration-effectiveness score (7 vs 7 points, P = 0.8), and neurocognitive diagnosis based on Frascati criteria and radiological findings., Conclusions: In this large pooled sample of PLWH with neurocognitive symptoms, CSF HIV-1 escape occurred in 8.7% of PLWH. People living with HIV with CSF HIV-1 escape presented no distinctive clinical or paraclinical characteristics. We conclude that lumbar puncture is unavoidable in confirming CSF HIV-1 escape., Competing Interests: P.F., J.D., B.V., F.A., O.N.T., P.T., T.D., M.O., I.J., T.H., and R.D.P. report no conflict of interest related to this work., (Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.)

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results