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Additional file 1: Figure S1. Comparison of the anti-coagulant impact on NC incorporation to the growing thrombi. Figure S2. Instant NC velocities and averaged velocities. Figure S3. Comparison between heparin and hirudin impact on NCs. Figure S4. Classification of the NC cells among the thrombi. Figure S5. Analysis of the CD11b/CD18 distribution in granulocytes and MNCs. Figure S6. The number of NCs per FOV increased gradually up to the 20th minute of the experiment. Figure S7. Blood cell composition changes after perfusion through flow chamber with fibrillar collagen or BSA. Figure S8. Impact of platelet depletion on granulocyte crawling velocity. Figure S9. Fucoidan impact on platelet P-selectin exposure in growing thrombi. Figure S10. Granulocyte movement around thrombi. Figure S11. Thrombus area and percentage of highly activated granulocytes in the presence of leukocyte activators and in patients with WAS. Figure S12. Crawling granulocytes bear Annexin-V positive platelets. Figure S13. Annexin-V and CD66b staining. Figure S14. Assay of the granulocyte crawling in the blood of WAS patients in the presence of healthy donor platelets. Table S1. LAL-test results for endotoxin characterization in the protein samples. Table S2. Calibration of the LAL-test system.