Search

Your search keyword '"Oceguera-Yanez, Fabian"' showing total 22 results
Start Over Author "Oceguera-Yanez, Fabian"
22 results on '"Oceguera-Yanez, Fabian"'

7. Cdc42 and mDia3 regulate microtubule attachment to kinetochores

Author

Yasuda, Shingo, Oceguera-Yanez, Fabian, Kato, Takayuki, Okamoto, Muneo, Yonemura, Shigenobu, Terada, Yasuhiko, Ishizaki, Toshimasa, and Narumiya, Shuh

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Shingo Yasuda [1, 2]; Fabian Oceguera-Yanez [1]; Takayuki Kato [1, 5]; Muneo Okamoto [1]; Shigenobu Yonemura [3]; Yasuhiko Terada [4]; Toshimasa Ishizaki [1]; Shuh Narumiya (corresponding author) [1] During [...]

Published
2004
Full Text
View/download PDF

9. Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives

Author

Oceguera-Yanez, Fabian, primary, Kim, Shin-Il, additional, Matsumoto, Tomoko, additional, Tan, Ghee Wan, additional, Xiang, Long, additional, Hatani, Takeshi, additional, Kondo, Takayuki, additional, Ikeya, Makoto, additional, Yoshida, Yoshinori, additional, Inoue, Haruhisa, additional, and Woltjen, Knut, additional

Published
2016
Full Text
View/download PDF

17. Inactivation of Rho GTPases with Clostridium difficileToxin B Impairs Centrosomal Activation of Aurora-A in G2/M Transition of HeLa Cells

Author

Ando, Yoshikazu, Yasuda, Shingo, Oceguera-Yanez, Fabian, and Narumiya, Shuh

Abstract

During G2 phase of cell cycle, centrosomes function as a scaffold for activation of mitotic kinases. Aurora-A is first activated at late G2 phase at the centrosome, facilitates centrosome maturation, and induces activation of cyclin B-Cdk1 at the centrosome for mitotic entry. Although several molecules including HEF1 and PAK are implicated in centrosomal activation of Aurora-A, signaling pathways leading to Aurora-A activation at the centrosome, and hence mitotic commitment in vertebrate cells remains largely unknown. Here, we have used Clostridium difficiletoxin B and examined the role of Rho GTPases in G2/M transition of HeLa cells. Inactivation of Rho GTPases by the toxin B treatment delayed by 2 h histone H3 phosphorylation, Cdk1/cyclin B activation, and Aurora-A activation. Furthermore, PAK activation at the centrosome that was already present before the toxin addition was significantly attenuated for 2 h by the addition of toxin B, and HEF1 accumulation at the centrosome that occurred in late G2 phase was also delayed. These results suggest that Rho GTPases function in G2/M transition of mammalian cells by mediating multiple signaling pathways converging to centrosomal activation of Aurora-A.

Published
2007
Full Text
View/download PDF

18. Inactivation of Rho GTPases with Clostridium difficile Toxin B Impairs Centrosomal Activation of Aurora-A in G2/M Transition of HeLa Cells

Author

Ando, Yoshikazu, Yasuda, Shingo, Oceguera-Yanez, Fabian, and Narumiya, Shuh

Abstract

During G2 phase of cell cycle, centrosomes function as a scaffold for activation of mitotic kinases. Aurora-A is first activated at late G2 phase at the centrosome, facilitates centrosome maturation, and induces activation of cyclin B-Cdk1 at the centrosome for mitotic entry. Although several molecules including HEF1 and PAK are implicated in centrosomal activation of Aurora-A, signaling pathways leading to Aurora-A activation at the centrosome, and hence mitotic commitment in vertebrate cells remains largely unknown. Here, we have used Clostridium difficile toxin B and examined the role of Rho GTPases in G2/M transition of HeLa cells. Inactivation of Rho GTPases by the toxin B treatment delayed by 2 h histone H3 phosphorylation, Cdk1/cyclin B activation, and Aurora-A activation. Furthermore, PAK activation at the centrosome that was already present before the toxin addition was significantly attenuated for 2 h by the addition of toxin B, and HEF1 accumulation at the centrosome that occurred in late G2 phase was also delayed. These results suggest that Rho GTPases function in G2/M transition of mammalian cells by mediating multiple signaling pathways converging to centrosomal activation of Aurora-A.

Published
2007

21. Actin Polymerization -- Driven Molecular Movement of mDia1in Living Cells.

Author

Higashida, Chiharu, Miyoshi, Takushi, Fujita, Akiko, Oceguera-Yanez, Fabian, Monypenny, James, Andou, Yoshikazu, Narumiya, Shuh, and Watanabe, Naoki

Subjects
*ACTIN, *POLYMERIZATION, *MOLECULAR structure, *PROTEINS, *NUCLEATION, *PHYSICAL & theoretical chemistry
Abstract

mDia1, a Rho effector, belongs to the Formin family of proteins, which shares the conserved tandem FH1-FH2 unit structure. Formins including mDia1 accelerate actin nucleation while interacting with actin filament fast-growing ends. Here our single-molecule imaging revealed fast directional movement of mDia1 FH1-FH2 for tens of microns in living cells. The movement of mDial FH1-FH2 was blocked by actin-perturbing drugs, and the speed of mDial FH1-FH2 movement appeared to correlate with actin elongation rates. In vitro, mDial FH1-FH2 associated persistently with the growing actin barbed end. mDial probably moves processively along the growing end of actin filaments in cells, and Formins may be a molecular motility machinery that is independent from motor proteins. [ABSTRACT FROM AUTHOR]

Published
2004
Full Text
View/download PDF

22. Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.

Author

Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, and Woltjen K

Subjects
Cell Separation methods, Cell Tracking methods, Cells, Cultured, Clone Cells cytology, Doxycycline pharmacology, Fibroblasts cytology, Gene Expression drug effects, Genes, Reporter, Green Fluorescent Proteins genetics, Humans, Induced Pluripotent Stem Cells metabolism, Luciferases genetics, Luminescent Proteins genetics, Neomycin pharmacology, Puromycin pharmacology, Red Fluorescent Protein, Cell Culture Techniques methods, Cellular Reprogramming, Cellular Reprogramming Techniques methods, DNA Transposable Elements genetics, Gene Expression genetics, Genetic Vectors genetics, Induced Pluripotent Stem Cells cytology, Transgenes genetics
Abstract

Transgenics is a mainstay of functional genomics. Conditionally overexpressing genes of interest (GOIs) helps to reveal their roles in the control of complex biological processes. Complemented by findings in classic animal model systems, recent advances in human embryonic stem cell (hESC) and patient-specific induced pluripotent stem cell (hiPSC) differentiation have led to sophisticated in vitro models of human development and disease. Yet, as transgenic elements encoding inducible systems must be introduced de novo into each genetically unique human stem cell line, robust and straightforward solutions to gene delivery are required. Transposons are a family of mobile DNA elements that have been adapted as experimental tools for stable genomic integration of transgenes. The piggyBac (PB) transposon from Trichoplusia ni presents a number of benefits over classic viral or BAC transgenesis: ease of application, simple integration-site mapping, and the unique capacity for traceless excision. Moreover, their large capacity permits the consolidation of multiple transgene components in a single vector system. In this chapter, we outline the features of a panel of "All-in-One" PB transposons designed for drug-inducible gene expression and provide guidelines to establish and validate populations or clones of transgenic hiPSCs.

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results