1. Occurrence of Arthroderma benhamiae genotype in Japan.
- Author
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Hiruma J, Kano R, Harada K, Monod M, Hiruma M, Hasegawa A, and Tsuboi R
- Subjects
- Animals, Arthrodermataceae classification, Dermatomycoses epidemiology, Genotype, Guinea Pigs microbiology, Humans, Japan epidemiology, Molecular Sequence Data, Octodon microbiology, Phylogeny, Polymerase Chain Reaction, Rabbits microbiology, Arthrodermataceae genetics, Arthrodermataceae isolation & purification, Dermatomycoses microbiology, Dermatomycoses veterinary
- Abstract
In this study, we epidemiologically investigated on clinical isolates of Arthroderma benhamiae from humans and animals in Japan by internal transcribed spacer (ITS) region sequence analysis and mating type (MAT)-specific PCR. Seven of 8 A. benhamiae isolates from a human, rabbits and guinea pigs were identified as group I (white phenotype) by morphological characters and ITS region sequence analysis. One strain isolated from a degus (Octodon degus) produced colonies with few irregular folds and yellow velvety mycelium without macro- and microconidia. This strain resembled to group II (yellow phenotype) strain. ITS sequence analysis was also 100% identical to that of group II. MAT-specific PCR indicated that 6 of these 7 isolates of group I contained an alpha-box gene and that one strain contained high-mobility-group (HMG) gene. One strain of group II was revealed to have an alpha-box gene and no HMG gene. To our knowledge, it is the first A. benhamiae isolate of group II found in Japan. The A. benhamiae may be more widespread in worldwide than our surpassing what is common or usual or expected.
- Published
- 2015
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